diff --git a/DataModel/Project/Core/Project-core-definition.yaml b/DataModel/Project/Core/Project-core-definition.yaml
index e69de29..d82ff36 100644
--- a/DataModel/Project/Core/Project-core-definition.yaml
+++ b/DataModel/Project/Core/Project-core-definition.yaml
@@ -0,0 +1,97 @@
+Project-core:
+  description: Describes the core details of the project like project ID, summary,
+    overall design, publication related details and any other supplementary links
+    associated with the project
+  properties:
+    properties:
+      project_id:
+        description: Primary key or unique identified for a project
+        type: string
+        pattern: "^DO-[0-9]{4}$"
+        example: "P034"
+      summary:
+        description: Summary of the experiment from which the project has been derived
+        type: string
+        example: "The identification of novel therapeutic strategies to overcome
+            the intrinsic or acquired resistance to trametinib in mutant KRAS lung
+            adenocarcinoma (LUAD) is a major challenge. This study analyzes the effects
+            of trametinib in Id1, a key factor involved in the oncogenic KRAS pathway,
+            and investigates the Id1 role in acquire resistance and synergy with immunotherapy
+            in KRAS-driven LUAD. Restoring the antitumor immune response by blocking
+            programmed-cell death protein 1 (PD-1) and programmed-cell death-ligand
+            1 (PD-L1) pathway represents a major breakthrough in non-small-cell lung
+            cancer (NSCLC) treatment. Nevertheless, a high proportion of LUAD patients
+            with KRAS alterations remain refractory to this therapy. Material and
+            Methods: To explore whether MEK1/2 inhibition reduces Id1 expression,
+            in vitro and in vivo experiments were conducted in KRAS-mutant NSCLC cells
+            and murine models. RNAseq analysis was performed to elucidate the pathways
+            involved in Id1 inhibition. Apoptosis and PD-L1 expression was measured
+            by flow cytometry. Synergy of trametinib combined with anti-PD1 was investigated
+            in KRAS-mutant LUAD mouse models. Results: Using preclinical syngeneic
+            KRAS-mutant lung cancer mouse models, we demonstrate that trametinib synergizes
+            with PD-1 blockade to reduce lung cancer progression and increase mice
+            overall survival. This antitumor activity was linked to the degradation
+            of Id1 via proteasome, and an enhanced INF-Y-mediated PD-L1 tumor cell
+            expression in KRAS-mutant tumor cells. This effect required CD8+ T cells,
+            boosted the intratumoral CD8+/Treg ratio, reducing the intratumoral Treg/CD4+
+            ratio. Conclusions: Our data may support the role of Id1 in the trametinib
+            antitumoral effect, sustaining the mitogen-activated protein kinases (MAPK)
+            signaling pathway involved in the trametinib acquired resistance cells
+            and sensitizing KRAS-mutant lung tumors to PD-1 inhibitors, through PD-L1
+            overexpression."
+      overall_design:
+        description: Overall design of the experiment as given by the author
+        type: string
+        example: "In order to investigate the molecular mechanisms involved in the
+            trametinib-mediated Id1 downregulation, a global transcriptomic profiling
+            using RNA sequencing analysis was performed on murine CMT167 and KLA lung
+            cancer cells treated or not with trametinib"
+      donor_derived_dataset:
+        description: Whether the dataset has been derived from a donor or not
+        type: boolean
+        enum:
+        - True
+        - False
+        - NA
+        ontology_link:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/HRAVS?p=classes&conceptid=https%3A%2F%2Fpurl.humanatlas.io%2Fvocab%2Fhravs%23HRAVS_1002101
+      organism:
+        description: The organism from which the samples were derived
+        type: string
+        example: "Homo sapiens"
+        ontology_link:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/ORTH?p=classes&conceptid=http%3A%2F%2Fpurl.org%2Fnet%2Forth%23Organism
+      cell_line:
+        description: Identifier of the cell lines from which the samples were derived for this experiment
+        type: string
+        pattern: "^CVCL_[0-9]{4}$"
+        example: "CVCL_0030"
+        ontology_link:
+          type: string
+          description: Link to the ontology file
+          url: https://www.cellosaurus.org/index.html
+      author_cell_type:
+        description: Cell types as curated from the publication/source for this experiment
+        type: string
+        example: "CMT167 cell"
+        ontology_link:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/CCF?p=classes&conceptid=http%3A%2F%2Fpurl.org%2Fccf%2FCellType
+      source_link:
+        description: Link to source from where the data was fetched given as a url
+        type: string
+        format: alphanumeric
+        example: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE236258
+      supplementary_links:
+        description: External link(s) pointing to code, supplementary data files,
+          or analysis files associated with the project which will not be uploaded
+        type: array
+        format: alphanumeric
+        example: https://github.com/czbiohub/tabula-muris, http://celltag.org/
+  type: object
+  version: 1.0.0
\ No newline at end of file
diff --git a/DataModel/Project/Protocol/Protocol-definition.yaml b/DataModel/Project/Protocol/Protocol-definition.yaml
index e69de29..b763f8d 100644
--- a/DataModel/Project/Protocol/Protocol-definition.yaml
+++ b/DataModel/Project/Protocol/Protocol-definition.yaml
@@ -0,0 +1,437 @@
+Protocol:
+  description: Describes the protocols followed in a study, including details such
+    as the cell capture system, library protocol, the chemistry kit and sampling technique
+    used, as well as sequencing related information.
+  properties:
+    core:
+      description: Core properties of the protocol
+      properties:
+      donor_id:
+        description: Foreign key or unique identifier to map to donor ID
+        type: string
+        pattern: "^DO-[0-9]{4}$"
+        example: "DO-31724"
+      project_id:
+        description: Foreign key or unique identifier to map a project
+        type: string
+        pattern: "^P[0-9]{3}$"
+        example: "P024"
+      protocol_id:
+        description: Primary key or unique identifier for the protocol ID
+        type: string
+        pattern: "^PRT[0-9]{3}$"
+        example: "PRT451"
+      protocol_name:
+        description: Describes the experiment procedure or methods organized in a standardized format that can be executed in a laboratory environment.
+        type: string
+        example: "Single cell sequencing protocol"
+      protocol_description:
+        description: Protocols define step-by-step procedures and include a list of the materials needed to perform the experiment.
+        type: string
+        example: "Prepare Media from stocks, Add 485 µl Clone Medium to ionomycin vial; add 490 µl Clone Medium to PMA vial, mix and transfer 5µl of diluted PMA to ionomycin vial yielding the final solution. Collect spleens from mice and place each in a small petri dish containing 5ml Clone Medium. Isolate lymphocytes using the syringe puncture method. Wash and lyse rbcs with ACT.
+          Wash with Clone Medium and resuspend in 2ml Clone Medium. Place exactly
+          1ml in one well of a 12 well plate. Add an additional 1ml of Clone Medium
+          to the well. Can store spleen suspensions in 12 well plate overnight in
+          refrigerator. Use remaining spleen cells for phenotype FACS if desired -
+          wash with Staining Buffer to remove Clone Medium."
+      protocol_core:
+        description: Core protocol-level information
+        type: string
+        example: "Prepare Media from stocks, Add 485 µl Clone Medium to ionomycin vial;
+          add 490 µl Clone Medium to PMA vial, mix and transfer 5µl of diluted PMA
+          to ionomycin vial yielding the final solution. Collect spleens from mice
+          and place each in a small petri dish containing 5ml Clone Medium. Isolate
+          lymphocytes using the syringe puncture method. Wash and lyse rbcs with ACT.
+          Wash with Clone Medium and resuspend in 2ml Clone Medium. Place exactly
+          1ml in one well of a 12 well plate. Add an additional 1ml of Clone Medium
+          to the well. Can store spleen suspensions in 12 well plate overnight in
+          refrigerator. Use remaining spleen cells for phenotype FACS if desired -
+          wash with Staining Buffer to remove Clone Medium."
+    analysis:
+      description: Analysis related information of the protocol
+      properties:
+      computational_method:
+        description: A URL to a versioned workflow and versioned execution environment
+        type: string
+        format: alphanumeric
+      matrix_transformation:
+        description: Information related to protocols that transforms a matrix
+        type: string
+        example: "r2"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/ATOM?p=classes&conceptid=http%3A%2F%2Furi.interlex.org%2Ftgbugs%2Furis%2Freadable%2Fatlas%2FTransformationMatrix
+      bioinformatics_software:
+        description: A wide array of computer programs and tools designed to manage, analyze, and interpret biological data
+        type: string
+        example: "Cellranger"
+        Ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/NGBO?p=classes&conceptid=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FNGBO_6000410
+      bioinformatics_software_version:
+        description: Version of software used
+        type: string
+        example: "v2.0.1"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/NCIT?p=classes&conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C111093
+      gene_annotation_version:
+        description: Ensembl release version accession number or NCBI RefSeq assembly
+          version used for gene annotation
+        type: string
+        example: "GCF_000001405.40"
+    sequencing:
+      description: Sequence related information of the protocol
+      properties:
+      chemistry_kit:
+        description: Chemistry/Reagent kit used in the protocol
+        type: string
+        example: "Ion Total RNASeq Kit v2"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/NCIT?p=classes&conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C42793
+      library_protocol:
+        description: Specifies the protocol for preparing sequencing libraries from
+          single-cell RNA or DNA, outlining the steps for library construction and
+          amplification.
+        type: string
+        example: "Stage I - Preparation of single-cell lysates"
+        enum:
+        - Stage I - Preparation of single-cell lysates
+        - Stage II - Lysate cleanup and reverse transcription of mRNA species
+        - Stage III - Performing whole transcriptome amplification) and post-PCR cleanup
+        - Stage IV - Nextera XT sequencing-library construction
+        - Stage V - Pooling and DNA SPRI bead cleanup
+        - Stage VI - Sequencing
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/NCIT?p=classes&conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C41111
+      sampling_technique:
+        description: The method/procedure used for collecting samples
+        type: string
+        example: "Biopsy"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/NCIT?p=classes&conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C71492
+      sequencer:
+        description: The sequencing platform used to generate single-cell sequencing
+          data.
+        type: string
+        example: "Illumina NexSeq 2000"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/BIRNLEX?p=classes&conceptid=http%3A%2F%2Fbioontology.org%2Fprojects%2Fontologies%2Fbirnlex%23birnlex_2408
+      sequencing_technology:
+        description: Describes the methodology employed for sequencing, such as single
+          cell RNA-seq, ATAC-seq, or DNA sequencing.
+        type: string
+        example: "scRNA-Seq"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/GFVO?p=classes&conceptid=https%3A%2F%2Fwww.codamono.com%2Fbiointerchange%2Fgfvo%23SequencingTechnologyPlatform
+    sample_collection:
+      description: Sample collection related information of the protocol
+      properties:
+      formation_method:
+        description: Method used to form cell aggregates
+        type: string
+        example: "suspension cultures"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/ENM?p=classes&conceptid=http%3A%2F%2Fpurl.enanomapper.org%2Fonto%2FENM_9000002
+      cell_uniformity:
+        description: Description of the cell aggregates uniformity after formation
+        type: string
+        example: Mostly homogenous embryoid bodies of variable cell numbers; Low-homogeneity
+          in morphology and size
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/IOBC?p=classes&conceptid=http%3A%2F%2Fpurl.jp%2Fbio%2F4%2Fid%2F201106089792994038
+      method_collection:
+        description: Method used to collect the biomaterial
+        type: string
+        example: "Biopsy"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/NCIT?p=classes&conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C70700
+      reagents:
+        description: A list of purchased reagents used in this protocol
+        type: array
+        example: "SMART CDS Primer II A, SMARTer II A Oligo, SMARTScribe Reverse Transcriptase"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/SNOMEDCT?p=classes&conceptid=http%3A%2F%2Fpurl.bioontology.org%2Fontology%2FSNOMEDCT%2F337340002
+      method_differentiation:
+        description: Method applied to cell culture to induce a specific differentiation
+          response
+        type: string
+        example: "Inductive Co-Culture"
+      media_differentiation:
+        description: Culture media used to induce a specific differentiation response
+        type: string
+        example: "StemPro-34 Serum-Free Medium"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/EFO?p=classes&conceptid=http%3A%2F%2Fwww.ebi.ac.uk%2Fefo%2FEFO_0000579
+      small_molecules:
+        description: Small molecules added to stem cell medium to induce a specific
+          differentiation response
+        type: string
+        example: "Retinoic Acid"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/EDAM?p=classes&conceptid=http%3A%2F%2Fedamontology.org%2Ftopic_0154
+      target_cell_yield:
+        description: Percent of target cells obtained after directed differentiation
+          of origin cell
+        type: float
+        format: numeric
+        example: 95
+        lower_value: 0.0
+      target_pathway:
+        description: Targeted pathway for specific differentiation response
+        type: string
+        example: "Hedgehog signaling pathway"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/NCIT/?p=classes&conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C20633
+      validation_method:
+        description: Method used to validate origin cell successfully differentiated
+          to target cell
+        type: string
+        example: "Pancreatic Cell DTZ Detection Assay"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/NCIT?p=classes&conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C115542
+      validation_result:
+        description: Result confirming successful differentiation to target cell type
+        type: string
+        example: "CD103 Positive"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/NCIT?p=classes&conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C16237
+      markers:
+        description: A list of markers used to enrich for or against certain cells
+        type: string
+        example: "CD4+ CD8-"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/LOINC?p=classes&conceptid=http%3A%2F%2Fpurl.bioontology.org%2Fontology%2FLNC%2FLP7783-6
+      minimum_size:
+        description: Minimum cell or organelle size passing selection, in microns
+        type: float
+        format: numeric
+        example: 70
+        upper_value: 10000.0
+        lower_value: 0.01
+        units:
+          description: Unit is a quantity of constant magnitude which is used to measure
+            the magnitudes of other quantities of the same manner
+          uom: µm
+          encoding: UTF-8
+      maximum_size:
+        description: Maximum cell or organelle size passing selection, in microns
+        type: float
+        format: numeric
+        example: 90
+        upper_value: 10000.0
+        lower_value: 0.01
+        units:
+          description: Unit is a quantity of constant magnitude which is used to measure
+            the magnitudes of other quantities of the same manner
+          uom: µm
+          encoding: UTF-8
+      method_induction:
+        description: Induction method applied to primary cell culture to induce pluripotent
+          stem cell generation
+        type: string
+        example: "lentivirus"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/NCIT?p=classes&conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C61367
+      reprogramming_factors:
+        description: Reprogramming factors added to primary cell culture to induce
+          pluripotency
+        type: string
+        example: "POU5F1"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/MESH?p=classes&conceptid=http%3A%2F%2Fpurl.bioontology.org%2Fontology%2FMESH%2FD065150
+      ipsc_induction_kit:
+        description: Kit used to induce pluripotent stem cell generation
+        type: string
+        example: "Human iPS Cell Reprogramming Episomal Kit"
+      pluripotency_test:
+        description: Description of how pluripotency was tested in induced pluripotent
+          stem cells
+        type: string
+        example: "Teratoma formation"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/PLOSTHES?p=classes&conceptid=http%3A%2F%2Flocalhost%2Fplosthes.2017-1%236180
+      percent_pluripotency:
+        description: Percent of iPSCs that passed the pluripotency test
+        type: float
+        format: numeric
+        example: 97.2
+        units:
+          description: Unit is a quantity of constant magnitude which is used to measure
+            the magnitudes of other quantities of the same manner
+          uom: '%'
+          encoding: UTF-8
+      pluripotency_vector_removed:
+        description: Whether a viral vector was removed after induction
+        type: boolean
+        enum:
+        - 'True'
+        - 'False'
+    imaging_preparation_protocol:
+      description: Image preperation related information of protocol
+      properties:
+      slice_thickness:
+        description: Thickness of the imaged slice in micrometres
+        type: integer
+        format: numeric
+        example: 14
+        units:
+          description: Unit is a quantity of constant magnitude which is used to measure
+            the magnitudes of other quantities of the same manner
+          uom: µm
+          encoding: UTF-8
+      internal_anatomical_structures:
+        description: Internal (landmark) structures visible in the overview image
+          that are informative about the broader anatomical context/location of the
+          sample
+        type: string
+        example: "M0"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/OCHV?p=classes&conceptid=http%3A%2F%2Fsbmi.uth.tmc.edu%2Fontology%2Fochv%2325102
+      fiducial_marker:
+        description: Fiducial markers for the alignment of images taken across multiple
+          rounds of imaging
+        type: string
+        example: "beads"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/NCIT?p=classes&conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C82602
+      expansion_factor:
+        description: Factor by which the imaged tissue was expanded in one dimension
+        type: integer
+        format: numeric
+        example: 3
+      microscope_setup_description:
+        description: Description of the microscope setup
+        type: string
+        example: "Motorized stage (SCAN IM 112 x 74, Marzhauser)"
+      microscopy_technique:
+        description: The type of microscopy
+        type: string
+        example: "Fluroscence"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/SNOMEDCT?p=classes&conceptid=http%3A%2F%2Fpurl.bioontology.org%2Fontology%2FSNOMEDCT%2F278289002
+      magnification:
+        description: Magnification of the objective used for imaging
+        type: string
+        example: "60x"
+      numerical_aperture:
+        description: Numerical aperture of the objective
+        type: float
+        format: numeric
+        example: 1.3
+      immersion_medium_type:
+        description: Immersion medium used for imaging
+        type: string
+        example: "oil"
+        ontology:
+          type: string
+          description: Link to the ontology file
+          url: https://bioportal.bioontology.org/ontologies/NCIT?p=classes&conceptid=http%3A%2F%2Fncicb.nci.nih.gov%2Fxml%2Fowl%2FEVS%2FThesaurus.owl%23C182013
+      immersion_medium_refractive_index:
+        description: Refractive index of the immersion medium used for imaging
+        type: float
+        format: numeric
+        example: 1.5
+      pixel_size:
+        description: Pixel size in nanometers
+        type: integer
+        format: numeric
+        example: 109
+        units:
+          description: Unit is a quantity of constant magnitude which is used to measure
+            the magnitudes of other quantities of the same manner
+          uom: nm
+          encoding: UTF-8
+      number_of_tiles:
+        description: Number of XY tiles in the experiment
+        type: integer
+        format: numeric
+        example: 2000
+      tile_size_y:
+        description: Y size of the tile in micrometers
+        type: integer
+        format: numeric
+        example: 100
+        units:
+          description: Unit is a quantity of constant magnitude which is used to measure
+            the magnitudes of other quantities of the same manner
+          uom: µm
+          encoding: UTF-8
+      tile_size_x:
+        description: X size of the tile in micrometers
+        type: integer
+        format: numeric
+        example: 100
+        units:
+          description: Unit is a quantity of constant magnitude which is used to measure
+            the magnitudes of other quantities of the same manner
+          uom: µm
+          encoding: UTF-8
+      z_stack_step_size:
+        description: Z-stack step size in nanometers
+        type: integer
+        format: numeric
+        example: 200
+        units:
+          description: Unit is a quantity of constant magnitude which is used to measure
+            the magnitudes of other quantities of the same manner
+          uom: nm
+          encoding: UTF-8
+      overlapping_tiles:
+        description: Whether tiles were collected with overlap
+        type: boolean
+      channel:
+        description: Information about each channel used in the imaging protocol
+        type: array
+        example: "red"
+      probe:
+        description: Information about each probe in the imaging experiment
+        type: array
+        example: "CNA35-OG488"
+  type: object
+  version: 1.0.0
\ No newline at end of file
diff --git a/DataModel/Project/Publication/Publication-definition.yaml b/DataModel/Project/Publication/Publication-definition.yaml
index e69de29..ede0741 100644
--- a/DataModel/Project/Publication/Publication-definition.yaml
+++ b/DataModel/Project/Publication/Publication-definition.yaml
@@ -0,0 +1,88 @@
+Publication:
+  description: Describes the details of the publication from which the experiment has been taken. Details include abtract, author information, pubication year and link
+  properties:
+    project_id:
+      description: Primary key or unique identified for a project
+      type: string
+      pattern: "^DO-[0-9]{4}$"
+      example: "P034"
+    abstract:
+      description: Abstract of the publication associated with the dataset.
+      type: string
+      example: "Background: The identification of novel therapeutic strategies to overcome resistance
+        to overcome resistance to the MEK inhibitor trametinib in mutant KRAS
+        lung adenocarcinoma (LUAD) is a challenge. This study analyzes the effects
+        of trametinib on Id1 protein, a key factor involved in the KRAS oncogenic
+        pathway, and investigates the role of Id1 in the acquired resistance to
+        trametinib as well as the synergistic anticancer effect of trametinib 
+        combined with immunotherapy in KRAS-mutant LUAD. Methods: We evaluated
+        the effects of trametinib on KRAS-mutant LUAD by Western blot, RNA-seq
+        and different syngeneic mouse models. Genetic modulation of Id1 expression
+        was performed in KRAS-mutant LUAD cells by lentiviral or retroviral transductions
+        of specific vectors. Cell viability was assessed by cell proliferation
+        and colony formation assays. PD-L1 expression and apoptosis were measured
+        by flow cytometry. The anti-tumor efficacy of the combined treatment with
+        trametinib and PD-1 blockade was investigated in KRAS-mutant LUAD mouse
+        models, and the effects on the tumor immune infiltrate were analyzed by
+        flow cytometry and immunohistochemistry. Results: We found that trametinib
+        activates the proteasome-ubiquitin system to downregulate Id1 in KRAS-mutant
+        LUAD tumors. Moreover, we found that Id1 plays a major role in the acquired
+        resistance to trametinib treatment in KRAS-mutant LUAD cells. Using two
+        preclinical syngeneic KRAS-mutant LUAD mouse models, we found that trametinib
+        synergizes with PD-1/PD-L1 blockade to hamper lung cancer progression
+        and increase survival. This anti-tumor activity depended on trametinib-mediated
+        Id1 reduction and was associated with a less immunosuppressive tumor microenvironment
+        and increased PD-L1 expression on tumor cells.Conclusions: Our data demonstrate
+        that Id1 expression is involved in the resistance to trametinib and in
+        the synergistic effect of trametinib with anti-PD-1 therapy in KRAS-mutant
+        LUAD tumors. These findings suggest a potential therapeutic approach for
+        immunotherapy-refractory KRAS-mutant lung cancers."
+    authors:
+      description: List of authors for the associated publication
+      type: string
+      example: "Puyalto A, Rodríguez-Remírez M, López I, Macaya I"
+    journal:
+      description: Journal in which the associated study was published
+      type: string
+      example: "Molecular Cancer"
+    publication_link:
+      description: Link to the publication associated with the dataset. If the
+        associated publication information is not available, then this field provides
+        the link to the data source providing more information on the dataset.
+      type: string
+      format: alphanumeric
+      example: https://pubmed.ncbi.nlm.nih.gov/38643157/
+    publication_title:
+      description: Title of the publication associated with this dataset
+      type: string
+      example: "Trametinib sensitizes KRAS-mutant lung adenocarcinoma tumors to
+       PD-1/PD-L1 axis blockade via Id1 downregulation"
+    publication_year:
+      description: Year in which the dataset was published
+      type: integer
+      format: numeric
+      example: 2023
+      lower_value: 1900
+      units:
+        description: Unit is a quantity of constant magnitude which is used to
+          measure the magnitudes of other quantities of the same manner
+        uom: year
+        encoding: UTF-8
+    pubmed_id:
+      description: Unique PUBMED identifier of the publication associated with
+        the dataset
+      type: integer
+      format: numeric
+      example: 38643157
+      ontology_link:
+        type: string
+        description: Link to the ontology file
+        url: https://bioportal.bioontology.org/ontologies/OBI?p=classes&conceptid=http%3A%2F%2Fpurl.obolibrary.org%2Fobo%2FOBI_0001617
+    total_number_of_cells:
+      description: Total number of cells in a dataset
+      type: integer
+      format: numeric
+      example: 150000
+      lower_value: 0.0
+  type: object
+  version: 1.0.0