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Limit scale of adjustments for microfluidic_chip button/chamber finding. #18

@palmhjell

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@palmhjell

For a MITOMI device, chambers spacing is near-perfectly regular, with the main deviations coming from (1) imperfect staging of the slide (e.g., giving rotation) and (2) stitching errors. These are generally very slight issues with only very small local adjustments needed from chamber to chamber, and mostly handled by pipeline arguments.

Right now, the protocol allows larger-than-reasonable adjustments to chambers (often thinking that part of the neck or sandwich is part of the chamber), see:

Image

where the chambers would have been found almost perfectly (yay!) if no (or far smaller) adjustments were made (boo).

It would be nice if (1) there were an argument to limit the scale of the adjustments made during button/chamber finding, and (2) if these adjustments could be limited locally (i.e., only i pixels from the center of the surrounding chambers), since chamber-to-chamber deviations are far smaller than global deviations across a chip.

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