data.txt

Sample	Replicate	Total	SubA	SubB
001	rep 1	84795	10003	258
001	rep 2	85302	10008	258
001	rep 3	84507	10006	252
002	rep 1	75061	10984	779
002	rep 2	74208	11007	760
002	rep 3	73866	11033	740
003	rep 1	34590	9720	169
003	rep 2	30936	9705	150
003	rep 3	31364	9697	142
005	rep 1	24872	10282	976
005	rep 2	24386	10290	1086
005	rep 3	24328	10253	1057
007	rep 1	47286	8127	327
007	rep 2	52703	8928	377
007	rep 3	53619	9530	401
008	rep 1	29578	10529	4001
008	rep 2	29352	7556	3368
008	rep 3	28819	10479	4041
009	rep 1	139774	9875	463
009	rep 2	115865	8174	431
009	rep 3	128105	9107	435
010	rep 1	94601	10000	1057
010	rep 2	92189	10000	991
010	rep 3	89044	10000	983
013	rep 1	62864	12673	355
013	rep 2	63236	12593	325
013	rep 3	60920	12123	329
014	rep 1	38746	9730	1775
014	rep 2	39418	9926	1898
014	rep 3	39583	9824	1750