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Error in lsiProjection #23

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pbrazda opened this issue Aug 24, 2020 · 0 comments
Open

Error in lsiProjection #23

pbrazda opened this issue Aug 24, 2020 · 0 comments

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@pbrazda
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pbrazda commented Aug 24, 2020

Hi ,

I'm having some trouble with the umapProjection from
'Update scRNA_02_Cluster_Disease_w_Reference_v1.R'
at line 366:
umapProjection <- uwot::umap_transform(as.matrix(lsiProjection$matSVD)[,1:25], umapManifold, verbose = TRUE)

The error is
Error in lsiProjection$matSVD : $ operator not defined for this S4 class
but I cannot see the matSVD slot here, not even within lsiProjection@ .

As I understand, it should be coming from
lsiProjection <- projectLSI(matProjectLSI, lsiReference)

I would like to use it to project a second scRNAseq dataset on a previously determined scRNAseq umap.

Thank you for any comments and tips!

Best,
Peter

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