diff --git a/Formula/p/pybind11.rb b/Formula/p/pybind11.rb
index dcfd513e5dd33..88946863c057b 100644
--- a/Formula/p/pybind11.rb
+++ b/Formula/p/pybind11.rb
@@ -1,8 +1,8 @@
 class Pybind11 < Formula
   desc "Seamless operability between C++11 and Python"
   homepage "https://github.com/pybind/pybind11"
-  url "https://github.com/pybind/pybind11/archive/refs/tags/v3.0.0.tar.gz"
-  sha256 "453b1a3e2b266c3ae9da872411cadb6d693ac18063bd73226d96cfb7015a200c"
+  url "https://github.com/pybind/pybind11/archive/refs/tags/v3.0.1.tar.gz"
+  sha256 "741633da746b7c738bb71f1854f957b9da660bcd2dce68d71949037f0969d0ca"
   license "BSD-3-Clause"
 
   livecheck do
@@ -11,7 +11,7 @@ class Pybind11 < Formula
   end
 
   bottle do
-    sha256 cellar: :any_skip_relocation, all: "66d50f1e916c452bfaa6e6212ab2f35777de43cbf68e9d7b6b299f1a503479bd"
+    sha256 cellar: :any_skip_relocation, all: "b166d6278d9e471fdd2d420fd33a790b764b69c3c1ff6098754517cd500a4d54"
   end
 
   depends_on "cmake" => :build
@@ -29,7 +29,6 @@ def install
     system "cmake", "--install", "build"
 
     # build an `:all` bottle.
-    inreplace share/"cmake/pybind11/FindPythonLibsNew.cmake", "/usr/local", HOMEBREW_PREFIX
     inreplace share/"pkgconfig/pybind11.pc", /^prefix=$/, "\\0#{opt_prefix}"
 
     pythons.each do |python|