GPR curation for Arachidonic acid metabolism (2)

[JHL-452b]

Current behavior:

29 genes are discovered to be uncorrelated with their corresponding reactions in Arachidonic acid metabolism

Gene	UniProtID	Reaction	GPR	Correlation	Manually check
ENSG00000108242	P33260	MAR00936: arachidonate + H+ + NADPH + O2 --> 13-HETE + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000108242 encodes a cytochrome P450 monooxygenase involved in retinoid metabolism, specifically hydroxylating all-trans-retinoic acid (atRA) to 4-hydroxyretinoate using molecular oxygen and electrons from NADPH. The provided reaction describes the conversion of arachidonate to 13-HETE, which does not involve retinoid metabolism or the specific enzymatic activity of the cytochrome P450 encoded by ENSG00000108242, indicating no relevance.	No Yes
ENSG00000186377	Q8N118	MAR00939: arachidonate + H+ + NADPH + O2 --> 18-HETE + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000186377 encodes a cytochrome P450 monooxygenase that primarily catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, and does not have hydroxylase activity toward various fatty acids, steroids, and prostaglandins. The provided reaction describes the conversion of arachidonate to 18-HETE, which involves hydroxylation rather than epoxidation. Hence, the gene's encoded enzyme does not match the described catalytic activity in the reaction.	No
ENSG00000160870	P24462	MAR00942: arachidonate + H+ + NADPH + O2 --> 5,6-EET + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000160870 encodes a cytochrome P450 monooxygenase which is involved in the metabolism of steroid hormones and vitamins. It catalyzes various hydroxylation reactions on substrates such as dehydroepiandrosterone (DHEA), estrone, testosterone, 17beta-estradiol, and all-trans-retinoate using molecular oxygen and NADPH via cytochrome P450 reductase. However, the provided reaction involves arachidonate, NADPH, and O2 to produce 5,6-EET, H2O, and NADP+. This reaction is not mentioned among the catalytic activities associated with the gene, indicating that the gene is not involved in this reaction.	No
ENSG00000137869	P11511	MAR00942: arachidonate + H+ + NADPH + O2 --> 5,6-EET + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000137869 encodes a cytochrome P450 monooxygenase, which catalyzes the conversion of C19 androgens such as androstenedione and testosterone into C18 estrogens like estrone and estradiol through successive oxidation steps. It also has activity toward estrone and dihydrotestosterone. In contrast, the reaction provided involves the conversion of arachidonate, H+, NADPH, and O2 to 5,6-EET, H2O, and NADP+, which is unrelated to the activities of the cytochrome P450 monooxygenase described.	No
ENSG00000106258	P20815	MAR00947: arachidonate + FADH2 + O2 --> 8,9-EET + FAD + H2O	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000106258 encodes a cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins, catalyzing reactions that involve the hydroxylation of carbon-hydrogen bonds and the oxidative metabolism of xenobiotics. The listed catalytic activities include the conversion of 17beta-estradiol, estrone, testosterone, progesterone, and all-trans-retinol, none of which correspond to the reaction provided: arachidonate + FADH2 + O2 --> 8,9-EET + FAD + H2O. The provided reaction involves the epoxidation of arachidonic acid to form 8,9-epoxyeicosatrienoic acid (EET), a process not associated with the function or catalytic activity of cytochrome P450 described for this gene.	No
ENSG00000160870	P24462	MAR00950: arachidonate + H+ + NADPH + O2 --> 11,12-EET + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000160870 encodes a cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins during embryogenesis, as well as the oxidative metabolism of xenobiotics. Its documented catalytic activities include hydroxylation of various organic substrates such as dehydroepiandrosterone, estrone, 17¦Â-estradiol, testosterone, and all-trans-retinoate. However, the provided reaction involves the conversion of arachidonate to 11,12-EET, which is not mentioned among the gene's known catalytic activities. Therefore, the gene ENSG00000160870 is not related to this particular reaction.	No
ENSG00000160870	P24462	MAR00951: arachidonate + FADH2 + O2 --> 11,12-EET + FAD + H2O	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000160870 encodes a cytochrome P450 monooxygenase that is involved primarily in the metabolism of steroid hormones and vitamins. It catalyzes the hydroxylation of carbon-hydrogen bonds in various substrates, including steroid hormones, retinoic acid, and xenobiotics. The specific reactions it catalyzes involve substrates such as 3beta-hydroxyandrost-5-en-17-one, dehydroepiandrosterone sulfate, estrone, testosterone, and all-trans-retinoate. The reaction provided, which converts arachidonate to 11,12-EET using FADH2 and O2, does not match the substrates or reaction mechanisms described for this gene. Therefore, the gene is not related to the provided reaction.	No
ENSG00000117592	P30041	MAR01045: 12(S)-HPETE <=> hepoxilin A3	ENSG00000116157 or ENSG00000117592 or ENSG00000164294 or ENSG00000167468 or ENSG00000176153 or ENSG00000198704 or ENSG00000211445 or ENSG00000224586 or ENSG00000233276	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000117592 encodes a thiol-specific peroxidase enzyme that primarily catalyzes the reduction of hydrogen peroxide and organic hydroperoxides, and exhibits phospholipase as well as lysophospholipid acyltransferase activities. These catalytic activities involve reactions with various substrates such as hydrogen peroxide, glutathione, diacylglycerol phosphocholine, and lysophosphatidylcholine. However, the reaction provided (12(S)-HPETE <=> hepoxilin A3) does not align with any of these functions or catalytic activities described for this gene. This reaction is not related to the described peroxidase, phospholipase, or acyltransferase activities of the encoded enzyme, indicating no relevance between the gene and the reaction.	No
ENSG00000224586	O75715	MAR01045: 12(S)-HPETE <=> hepoxilin A3	ENSG00000116157 or ENSG00000117592 or ENSG00000164294 or ENSG00000167468 or ENSG00000176153 or ENSG00000198704 or ENSG00000211445 or ENSG00000224586 or ENSG00000233276	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000224586 encodes a protein that protects cells and enzymes from oxidative damage by catalyzing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using glutathione. The catalytic activity mentioned involves converting hydrogen peroxide and glutathione into water and glutathione disulfide. However, the reaction provided (12(S)-HPETE <=> hepoxilin A3) is unrelated to this function as it does not involve hydrogen peroxide or glutathione, thus the gene is not relevant to the reaction.	No
ENSG00000176153	P18283	MAR01048: 12(S)-HPETE <=> hepoxilin A3	ENSG00000116157 or ENSG00000117592 or ENSG00000164294 or ENSG00000167468 or ENSG00000176153 or ENSG00000198704 or ENSG00000211445 or ENSG00000224586 or ENSG00000233276	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000176153 encodes an enzyme that catalyzes the reduction of various hydroperoxides in the presence of glutathione but does not catalyze the conversion of 12(S)-HPETE to hepoxilin A3. This specific reaction is not listed among the catalytic activities associated with the gene, as it typically involves reduction reactions involving 2 glutathione and various hydroperoxides, not the conversion of 12(S)-HPETE.	No
ENSG00000119673	P49753	MAR01065: 11,12-EET + ATP + CoA + 2.0 H2O + NAD+ + O2 --> 9,10-epoxy-(6Z,12Z)-octadecadienoic acid + acetyl-CoA + AMP + H2O2 + NADH + PPi	ENSG00000060971 or ENSG00000072506 or ENSG00000084754 or ENSG00000087008 or ENSG00000097021 or ENSG00000101473 or ENSG00000119673 or ENSG00000127884 or ENSG00000130304 or ENSG00000133835 or ENSG00000136881 or ENSG00000138029 or ENSG00000138796 or ENSG00000140284 or ENSG00000143554 or ENSG00000157426 or ENSG00000161533 or ENSG00000167107 or ENSG00000167114 or ENSG00000167315 or ENSG00000176715 or ENSG00000177465 or ENSG00000184227 or ENSG00000242110	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000119673 encodes an enzyme that catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), with higher activity toward long-chain acyl CoAs (C14-C20). It enhances hepatic fatty acid oxidation in mitochondria. The gene's catalytic activities involve reactions converting various acyl-CoAs, such as hexadecanoyl-CoA, tetradecanoyl-CoA, and octadecanoyl-CoA, into their respective fatty acids and CoA. In contrast, the provided reaction involves the transformation of 11,12-EET, ATP, CoA, H2O, NAD+, and O2 into 9,10-epoxy-(6Z,12Z)-octadecadienoic acid, acetyl-CoA, AMP, H2O2, NADH, and PPi, which is significantly different from the enzymatic activities documented for the gene. Therefore, the gene does not relate to this reaction.	No
ENSG00000072506	Q99714	MAR01069: 12,13-epoxy-(6Z,9Z)-octadecadienoic acid + ATP + CoA + 2.0 H2O + NAD+ + O2 --> 10,11-epoxy-(4Z,7Z)-hexadecadienoic acid + acetyl-CoA + AMP + 2.0 H+ + H2O2 + NADH + PPi	ENSG00000060971 or ENSG00000072506 or ENSG00000084754 or ENSG00000087008 or ENSG00000097021 or ENSG00000101473 or ENSG00000119673 or ENSG00000127884 or ENSG00000130304 or ENSG00000133835 or ENSG00000136881 or ENSG00000138029 or ENSG00000138796 or ENSG00000140284 or ENSG00000143554 or ENSG00000157426 or ENSG00000161533 or ENSG00000167107 or ENSG00000167114 or ENSG00000167315 or ENSG00000176715 or ENSG00000177465 or ENSG00000184227 or ENSG00000242110	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000072506 encodes a mitochondrial dehydrogenase involved in various metabolic pathways such as fatty acid beta-oxidation, branched-chain amino acid catabolism, and steroid metabolism. The enzyme catalyzes the oxidation of specific substrates like (S)-3-hydroxyacyl-CoA, 3-hydroxy-2-methylbutanoyl-CoA, and hydroxysteroids. In contrast, the provided reaction involves substrates such as 12,13-epoxy-(6Z,9Z)-octadecadienoic acid, ATP, and CoA, which are not substrates recognized or catalyzed by the enzyme encoded by this gene. Therefore, the gene's functions do not align with the specific reaction described.	No
ENSG00000186377	Q8N118	MAR00931: arachidonate + H+ + NADPH + O2 --> 7-HETE + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000186377 encodes a cytochrome P450 monooxygenase that specifically catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide. However, the reaction in question involves the conversion of arachidonate, H+, NADPH, and O2 to 7-HETE, H2O, and NADP+, which does not match the described catalytic activity of the gene. The gene does not exhibit hydroxylase activity toward various fatty acids, including the formation of 7-HETE from arachidonate, thus it is not relevant to the provided reaction.	No
ENSG00000108242	P33260	MAR00931: arachidonate + H+ + NADPH + O2 --> 7-HETE + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000108242 encodes a cytochrome P450 monooxygenase involved in retinoid metabolism, specifically hydroxylating all-trans-retinoic acid to 4-hydroxyretinoate. The catalytic activities mentioned involve the transformation of retinoic acid and the use of molecular oxygen, NADPH, and cytochrome P450 reductase. However, the provided reaction involves the conversion of arachidonate to 7-HETE, which is related to arachidonic acid metabolism, not retinoid metabolism. Therefore, the gene's function and the provided reaction do not coincide.	No Yes
ENSG00000106258	P20815	MAR00935: arachidonate + H+ + NADPH + O2 --> 11-HETE + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000106258 encodes a cytochrome P450 monooxygenase that primarily catalyzes the hydroxylation of steroid hormones and the oxidative metabolism of vitamins and various xenobiotics. However, it does not catalyze the conversion of arachidonate to 11-HETE as described in the provided reaction. The described gene's activities involve different substrates and reactions, including the formation of catechol estrogens, hydroxylation of steroid hormones like testosterone and progesterone, and metabolism of all-trans-retinol and all-trans-retinoic acid, but not the synthesis of 11-HETE from arachidonate.	No Yes
ENSG00000160870	P24462	MAR00936: arachidonate + H+ + NADPH + O2 --> 13-HETE + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The provided gene ENSG00000160870 encodes a cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins. It catalyzes the hydroxylation of various substrates such as dehydroepiandrosterone, testosterone, estrone, and all-trans-retinoic acid, but there is no mention of its involvement in the metabolism of arachidonate or the production of 13-HETE. Therefore, the gene is not related to the reaction where arachidonate is converted to 13-HETE.	No Yes
ENSG00000108242	P33260	MAR00939: arachidonate + H+ + NADPH + O2 --> 18-HETE + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000108242 encodes a cytochrome P450 monooxygenase involved in the hydroxylation of all-trans-retinoic acid to 4-hydroxyretinoate, primarily modulating retinoid metabolism using molecular oxygen and NADPH. However, the reaction provided involves the conversion of arachidonate to 18-HETE, which is a different pathway and substrate not involved with the activities of the gene ENSG00000108242. Therefore, the gene and the reaction are not related.	No
ENSG00000197408	P20813	MAR00940: arachidonate + H+ + NADPH + O2 --> 19(S)-HETE + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000197408 encodes a cytochrome P450 monooxygenase that is involved in the metabolism of endocannabinoids, steroids, and other xenobiotics. The reactions catalyzed by this enzyme predominantly involve the epoxidation of double bonds in arachidonoylethanolamide and hydroxylation of steroids such as testosterone and estrogens. However, the provided reaction involves the conversion of arachidonate to 19(S)-HETE, which is not among the activities listed for the gene's product. Therefore, the gene ENSG00000197408 is not related to the specified reaction.	No
ENSG00000137869	P11511	MAR00943: arachidonate + FADH2 + O2 --> 5,6-EET + FAD + H2O	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000137869 encodes a cytochrome P450 monooxygenase involved in the oxidation of C19 androgens to C18 estrogens. It catalyzes transformations involving androstenedione, testosterone, and other steroids through successive oxidations. However, the given reaction involves the conversion of arachidonate using FADH2 and oxygen to produce 5,6-EET, FAD, and water, which does not involve steroid hydroxylation or oxidation by cytochrome P450 and is therefore unrelated to the gene's annotated functions.	No
ENSG00000255974	P11509	MAR00944: arachidonate + FADH2 + O2 --> 5,6-EET + FAD + H2O	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The annotation of the gene ENSG00000255974 describes its function as involving coumarin 7-hydroxylase activity, hydroxylation of anti-cancer drugs such as cyclophosphamide and ifosphamide, metabolic activation of aflatoxin B1, nicotine C-oxidase activity, and 1,4-cineole 2-exo-monooxygenase activity. The provided reaction involves the conversion of arachidonate to 5,6-EET, which falls outside the described functions and activities of the gene. Therefore, the gene ENSG00000255974 is not related to the reaction provided.	No
ENSG00000108242	P33260	MAR00946: arachidonate + H+ + NADPH + O2 --> 8,9-EET + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000108242 encodes for a cytochrome P450 monooxygenase involved in retinoid metabolism, specifically hydroxylating all-trans-retinoic acid (atRA) to 4-hydroxyretinoate and modulating atRA signaling and clearance. The described catalytic activities involve atRA and molecular oxygen, producing retinoid metabolites with the help of NADPH via cytochrome P450 reductase. In contrast, the provided reaction converts arachidonate into 8,9-EET using NADPH and O2, indicating a different substrate and product, highlighting no functional connection to retinoid metabolism.	No
ENSG00000137869	P11511	MAR00948: arachidonate + FADH2 + O2 --> 8,9-EET + FAD + H2O	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000137869 encodes a cytochrome P450 monooxygenase involved in the oxidation of androgens such as androstenedione and testosterone to estrogens like estrone and estradiol. It catalyzes several specific reactions, including the formation of 19-hydroxy, 19-oxo derivatives, and oxidative aromatization. In contrast, the provided reaction involves the conversion of arachidonate to 8,9-EET using FADH2 and oxygen, which is distinctly different in both substrate and product, involving an epoxidation rather than androgen metabolism. Thus, the gene does not participate in the reaction described.	No
ENSG00000106258	P20815	MAR00951: arachidonate + FADH2 + O2 --> 11,12-EET + FAD + H2O	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000106258 encodes a cytochrome P450 monooxygenase involved in the metabolism of steroid hormones and vitamins, catalyzing various hydroxylation reactions using NADPH and molecular oxygen. The described catalytic activities of the gene product include hydroxylation of steroids, metabolism of retinoids, and oxidative metabolism of xenobiotics. However, the provided reaction involves the conversion of arachidonate to 11,12-EET using FADH2 and does not align with the gene's known functions and catalysis mechanisms, which predominantly involve NADPH and molecular oxygen.	No
ENSG00000116157	Q96SL4	MAR01043: 12(S)-HPETE <=> hepoxilin A3	ENSG00000116157 or ENSG00000117592 or ENSG00000164294 or ENSG00000167468 or ENSG00000176153 or ENSG00000198704 or ENSG00000211445 or ENSG00000224586 or ENSG00000233276	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000116157 has a function related to protecting esophageal epithelia from oxidative stress and catalyzing the reduction of hydrogen peroxide using glutathione. However, the reaction mentioned (12(S)-HPETE <=> hepoxilin A3) involves a different biochemical process that is not related to the gene's described catalytic activity or function.	No
ENSG00000116157	Q96SL4	MAR01049: 12(S)-HPETE <=> hepoxilin A3	ENSG00000116157 or ENSG00000117592 or ENSG00000164294 or ENSG00000167468 or ENSG00000176153 or ENSG00000198704 or ENSG00000211445 or ENSG00000224586 or ENSG00000233276	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000116157 is involved in protecting esophageal epithelia from oxidative stress and managing reactive oxygen species through a specific catalytic activity involving glutathione and hydrogen peroxide. However, the provided reaction, which involves the interconversion of 12(S)-HPETE to hepoxilin A3, does not align with the described functions or catalytic activity of the gene. Therefore, they are not related based on the given annotations.	No
ENSG00000211445	P22352	MAR01049: 12(S)-HPETE <=> hepoxilin A3	ENSG00000116157 or ENSG00000117592 or ENSG00000164294 or ENSG00000167468 or ENSG00000176153 or ENSG00000198704 or ENSG00000211445 or ENSG00000224586 or ENSG00000233276	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000211445 encodes for an enzyme that protects cells from oxidative damage by catalyzing the reduction of peroxide compounds, such as hydrogen peroxide and lipid peroxides, using glutathione. The described catalytic activities involve reactions with hydrogen peroxide and tert-butyl hydroperoxide, transforming them into non-toxic products. However, the reaction "12(S)-HPETE <=> hepoxilin A3" does not involve these substrates or products and does not fall under the catalytic activities associated with the gene. Therefore, the gene is not relevant to the reaction provided.	No
ENSG00000242110	Q9UHK6	MAR01067: 7,8-epoxy-(4Z,10Z)-hexadecadienoic acid + ATP + CoA + 2.0 H2O + NAD+ + O2 --> 5,6-epoxy-(8Z)-tetradecenoic acid + acetyl-CoA + AMP + H2O2 + NADH + PPi	ENSG00000060971 or ENSG00000072506 or ENSG00000084754 or ENSG00000087008 or ENSG00000097021 or ENSG00000101473 or ENSG00000119673 or ENSG00000127884 or ENSG00000130304 or ENSG00000133835 or ENSG00000136881 or ENSG00000138029 or ENSG00000138796 or ENSG00000140284 or ENSG00000143554 or ENSG00000157426 or ENSG00000161533 or ENSG00000167107 or ENSG00000167114 or ENSG00000167315 or ENSG00000176715 or ENSG00000177465 or ENSG00000184227 or ENSG00000242110	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000242110 encodes an enzyme that catalyzes the interconversion of stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters and acts specifically on coenzyme A thioesters, such as pristanoyl-CoA and ibuprofen derivatives. The provided reaction involves epoxide metabolism with substrates like epoxy fatty acids, ATP, CoA, H2O, NAD+, and O2, leading to products including acetyl-CoA, AMP, H2O2, NADH, and PPi. This reaction does not involve the stereoisomers or specific CoA thioesters that the encoded enzyme targets, indicating no relevance between the gene and the provided reaction.	No
ENSG00000127884	P30084	MAR01068: 14,15-EET + ATP + CoA + 2.0 H2O + NAD+ + O2 --> 12,13-epoxy-(6Z,9Z)-octadecadienoic acid + acetyl-CoA + AMP + H2O2 + NADH + PPi	ENSG00000060971 or ENSG00000072506 or ENSG00000084754 or ENSG00000087008 or ENSG00000097021 or ENSG00000101473 or ENSG00000119673 or ENSG00000127884 or ENSG00000130304 or ENSG00000133835 or ENSG00000136881 or ENSG00000138029 or ENSG00000138796 or ENSG00000140284 or ENSG00000143554 or ENSG00000157426 or ENSG00000161533 or ENSG00000167107 or ENSG00000167114 or ENSG00000167315 or ENSG00000176715 or ENSG00000177465 or ENSG00000184227 or ENSG00000242110	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000127884 encodes an enzyme that primarily catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters and exhibits high specificity for substrates like crotonyl-CoA, acryloyl-CoA, 3-methylcrotonyl-CoA, and methacrylyl-CoA. It is involved in the beta-oxidation pathway of fatty acids. The provided reaction, however, involves the metabolism of 14,15-EET and includes steps such as ATP consumption and production of acetyl-CoA, AMP, H2O2, NADH, and PPi, which are not related to the known catalytic activities of the gene's enzyme product. Therefore, the reaction does not align with the enzymatic functions described for this gene.	No
ENSG00000149084	Q53GQ0	MAR01074: 12(S)-HETE + H+ + NADH <=> 12-hetre + NAD+	ENSG00000106853 or ENSG00000149084 or ENSG00000205678	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000149084 encodes for an enzyme that primarily functions in the elongation cycle of long-chain and very long-chain fatty acids. It catalyzes multiple reactions involving the reduction of 3-ketoacyl-CoA to 3-hydroxyacyl-CoA and also plays a role in estrogen formation by converting estrone to estradiol. However, the provided reaction involves the interconversion of 12(S)-HETE and 12-hetre in the presence of NADH and NAD+. This reaction is related to a different pathway, likely involving hydroxyeicosatetraenoic acids (HETEs), which is not associated with the fatty acid elongation cycle or estrogen formation catalyzed by the enzyme encoded by the gene ENSG00000149084. Therefore, based on the annotations provided, the gene and the reaction are not related.	No

[JHL-452b]

For Arachidonic acid metabolism, I continued to add 3 unrelated gene-reaction pairs discoverd by GPT.

Gene	UniProtID	Reaction	GPR	Correlation	Check
ENSG00000137869	P11511	MAR00938: arachidonate + H+ + NADPH + O2 --> 17-HETE + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000137869 encodes a cytochrome P450 monooxygenase involved in the conversion of C19 androgens like androstenedione and testosterone into C18 estrogens such as estrone and estradiol. It catalyzes these transformations through oxidations involving hydrogen abstraction and bond cleavage. However, the provided reaction involves the conversion of arachidonate to 17-HETE, which is not related to the enzymatic pathways described for this cytochrome P450. Therefore, the gene's function and the specified reaction are not related.	No
ENSG00000137869	P11511	MAR00939: arachidonate + H+ + NADPH + O2 --> 18-HETE + H2O + NADP+	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. The gene ENSG00000137869 encodes a cytochrome P450 monooxygenase involved in the conversion of androgens to estrogens and other related oxidative processes. The catalytic activities listed involve substrates such as testosterone, androst-4-ene-3,17-dione, and various steroid derivatives, utilizing molecular oxygen and NADPH. In contrast, the provided reaction involves the conversion of arachidonate to 18-HETE. This is not related to the steroid metabolism pathways catalyzed by the cytochrome P450 monooxygenase and involves different substrates and products, indicating no relevance between the gene function and the reaction described.	No
ENSG00000106258	P20815	MAR00948: arachidonate + FADH2 + O2 --> 8,9-EET + FAD + H2O	ENSG00000021461 or ENSG00000100197 or ENSG00000106258 or ENSG00000108242 or ENSG00000130649 or ENSG00000134716 or ENSG00000137869 or ENSG00000138061 or ENSG00000138109 or ENSG00000138115 or ENSG00000140465 or ENSG00000140505 or ENSG00000142973 or ENSG00000155016 or ENSG00000160868 or ENSG00000160870 or ENSG00000165841 or ENSG00000167600 or ENSG00000171903 or ENSG00000186160 or ENSG00000186204 or ENSG00000186377 or ENSG00000186526 or ENSG00000197408 or ENSG00000197446 or ENSG00000197838 or ENSG00000198077 or ENSG00000255974	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000106258 encodes a cytochrome P450 monooxygenase that is involved in the metabolism of steroid hormones and vitamins, including the hydroxylation of carbon-hydrogen bonds and the oxidative metabolism of diverse substrates such as 17beta-estradiol, estrone, testosterone, progesterone, and all-trans-retinol. These activities involve inserting one oxygen atom into a substrate and reducing another into a water molecule with electrons provided by NADPH. However, the described reaction involves conversion of arachidonate to 8,9-EET with FADH2 and O2, which does not match the metabolic functions or catalytic activities of the gene product. Thus, based on the annotation, the gene and the reaction are not related.	No

[hhl-24]

keep ENSG00000108242（an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H+） for MAR00936: arachidonate + H+ + NADPH + O2 --> 13-HETE + H2O + NADP+，MAR00931: arachidonate + H+ + NADPH + O2 --> 7-HETE + H2O + NADP+，MAR00939: arachidonate + H+ + NADPH + O2 --> 18-HETE + H2O + NADP+
keep ENSG00000106258（an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H+）for MAR00935: arachidonate + H+ + NADPH + O2 --> 11-HETE + H2O + NADP+
keep ENSG00000160870（an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H+）MAR00936: arachidonate + H+ + NADPH + O2 --> 13-HETE + H2O + NADP+

[JHL-452b]

See #853

[hhl-24]

See #853

Refer to this issue, I suggest that remove ENSG00000186377(Has no hydroxylase activity toward various fatty acids) from MAR00939: arachidonate + H+ + NADPH + O2 --> 18-HETE + H2O + NADP+ and MAR00931: arachidonate + H+ + NADPH + O2 --> 7-HETE + H2O + NADP+. Remove ENSG00000242110(Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs but neither 3-methyl-branched nor linear-chain acyl-CoAs) from MAR01067: 7,8-epoxy-(4Z,10Z)-hexadecadienoic acid + ATP + CoA + 2.0 H2O + NAD+ + O2 --> 5,6-epoxy-(8Z)-tetradecenoic acid + acetyl-CoA + AMP + H2O2 + NADH + PPi. Others can be retained.

[JHL-452b]

Fixed by #962