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0.1.0 branch questions #67
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Hi, Thank you for using tailfindr. Here are answers to your questions:
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Hi Adnan, Thanks for the detailed reply!
yes I do have a non-polyA sequence attached to a polyA tail (that is supposed to be A only), and I get a 'polya_tail_fasta_seq' output that is only ~70% A. Is the nanopore basecalling this noisy/error-prone? For clarity, I am trying to compare a tailing reaction with ATP vs mixed nucleotides and trying to assess tail content. Thank you for all your help!! |
Hi Adnan,
Thank you so much for the great software! I have been trying to use it to look at the content of the polyA tails in my reaction.
I have a few questions:
Thank you so much.
Yours,
Ha An
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