diff --git a/crates/uv-python/src/lib.rs b/crates/uv-python/src/lib.rs
index 982878ff3d56b..cca022a1afbd0 100644
--- a/crates/uv-python/src/lib.rs
+++ b/crates/uv-python/src/lib.rs
@@ -20,7 +20,10 @@ pub use crate::version_files::{
     DiscoveryOptions as VersionFileDiscoveryOptions, FilePreference as VersionFilePreference,
     PythonVersionFile, PYTHON_VERSIONS_FILENAME, PYTHON_VERSION_FILENAME,
 };
-pub use crate::virtualenv::{Error as VirtualEnvError, PyVenvConfiguration, VirtualEnvironment};
+pub use crate::virtualenv::{
+    virtualenv_from_env, virtualenv_from_working_dir, Error as VirtualEnvError,
+    PyVenvConfiguration, VirtualEnvironment,
+};
 
 mod cpuinfo;
 mod discovery;
diff --git a/crates/uv-python/src/virtualenv.rs b/crates/uv-python/src/virtualenv.rs
index 5fdfc094f0291..fe48b1b858b8b 100644
--- a/crates/uv-python/src/virtualenv.rs
+++ b/crates/uv-python/src/virtualenv.rs
@@ -49,7 +49,7 @@ pub enum Error {
 /// Locate an active virtual environment by inspecting environment variables.
 ///
 /// Supports `VIRTUAL_ENV`.
-pub(crate) fn virtualenv_from_env() -> Option<PathBuf> {
+pub fn virtualenv_from_env() -> Option<PathBuf> {
     if let Some(dir) = env::var_os(EnvVars::VIRTUAL_ENV).filter(|value| !value.is_empty()) {
         return Some(PathBuf::from(dir));
     }
@@ -114,7 +114,7 @@ pub(crate) fn conda_environment_from_env(kind: CondaEnvironmentKind) -> Option<P
 /// Searches for a `.venv` directory in the current or any parent directory. If the current
 /// directory is itself a virtual environment (or a subdirectory of a virtual environment), the
 /// containing virtual environment is returned.
-pub(crate) fn virtualenv_from_working_dir() -> Result<Option<PathBuf>, Error> {
+pub fn virtualenv_from_working_dir() -> Result<Option<PathBuf>, Error> {
     let current_dir = crate::current_dir()?;
 
     for dir in current_dir.ancestors() {
diff --git a/crates/uv-settings/src/lib.rs b/crates/uv-settings/src/lib.rs
index 7d655bda3cf79..39c952431acd7 100644
--- a/crates/uv-settings/src/lib.rs
+++ b/crates/uv-settings/src/lib.rs
@@ -5,6 +5,7 @@ use std::path::{Path, PathBuf};
 use etcetera::BaseStrategy;
 
 use uv_fs::Simplified;
+use uv_python::{virtualenv_from_env, virtualenv_from_working_dir};
 use uv_static::EnvVars;
 use uv_warnings::warn_user;
 
@@ -55,6 +56,14 @@ impl FilesystemOptions {
         }
     }
 
+    pub fn venv() -> Result<Option<Self>, Error> {
+        let Some(file) = venv_config_file() else {
+            return Ok(None);
+        };
+        tracing::debug!("Found venv configuration in: `{}`", file.display());
+        Ok(Some(Self(read_file(&file)?)))
+    }
+
     pub fn system() -> Result<Option<Self>, Error> {
         let Some(file) = system_config_file() else {
             return Ok(None);
@@ -241,6 +250,26 @@ fn system_config_file() -> Option<PathBuf> {
     }
 }
 
+/// Returns the path to a venv-specific configuration file if one exists.
+fn venv_config_file() -> Option<PathBuf> {
+    // First check VIRTUAL_ENV environment variable
+    if let Some(venv_path) = virtualenv_from_env() {
+        let venv_config = PathBuf::from(venv_path).join("uv.toml");
+        if venv_config.is_file() {
+            return Some(venv_config);
+        }
+    }
+
+    // Then look for .venv in current or parent directories
+    if let Ok(Some(venv_path)) = virtualenv_from_working_dir() {
+        let venv_config = venv_path.join("uv.toml");
+        if venv_config.is_file() {
+            return Some(venv_config);
+        }
+    }
+
+    None
+}
 /// Load [`Options`] from a `uv.toml` file.
 fn read_file(path: &Path) -> Result<Options, Error> {
     let content = fs_err::read_to_string(path)?;
diff --git a/crates/uv/src/lib.rs b/crates/uv/src/lib.rs
index 0ac73dc4f3018..786075ab4c8e6 100644
--- a/crates/uv/src/lib.rs
+++ b/crates/uv/src/lib.rs
@@ -124,15 +124,19 @@ async fn run(mut cli: Cli) -> Result<ExitStatus> {
     } else if let Ok(workspace) =
         Workspace::discover(&project_dir, &DiscoveryOptions::default()).await
     {
+        // Combine in order of precedence: project -> venv -> user -> system
         let project = FilesystemOptions::find(workspace.install_path())?;
         let system = FilesystemOptions::system()?;
+        let venv = FilesystemOptions::venv()?;
         let user = FilesystemOptions::user()?;
-        project.combine(user).combine(system)
+        project.combine(venv).combine(user).combine(system)
     } else {
+        // Same precedence for non-workspace projects
         let project = FilesystemOptions::find(&project_dir)?;
         let system = FilesystemOptions::system()?;
+        let venv = FilesystemOptions::venv()?;
         let user = FilesystemOptions::user()?;
-        project.combine(user).combine(system)
+        project.combine(venv).combine(user).combine(system)
     };
 
     // Parse the external command, if necessary.
diff --git a/docs/configuration/files.md b/docs/configuration/files.md
index 05dcbdb35f094..b5bac200f0602 100644
--- a/docs/configuration/files.md
+++ b/docs/configuration/files.md
@@ -43,21 +43,23 @@ default = true
     `[tool.uv]` section in the accompanying `pyproject.toml` will be ignored.
 
 uv will also discover user-level configuration at `~/.config/uv/uv.toml` (or
-`$XDG_CONFIG_HOME/uv/uv.toml`) on macOS and Linux, or `%APPDATA%\uv\uv.toml` on Windows; and
-system-level configuration at `/etc/uv/uv.toml` (or `$XDG_CONFIG_DIRS/uv/uv.toml`) on macOS and
-Linux, or `%SYSTEMDRIVE%\ProgramData\uv\uv.toml` on Windows.
-
-User-and system-level configuration must use the `uv.toml` format, rather than the `pyproject.toml`
-format, as a `pyproject.toml` is intended to define a Python _project_.
-
-If project-, user-, and system-level configuration files are found, the settings will be merged,
-with project-level configuration taking precedence over the user-level configuration, and user-level
-configuration taking precedence over the system-level configuration. (If multiple system-level
+`$XDG_CONFIG_HOME/uv/uv.toml`) on macOS and Linux, or `%APPDATA%\uv\uv.toml` on Windows;
+venv-level configuration at within the virtual environment directory; and system-level
+configuration at `/etc/uv/uv.toml` (or `$XDG_CONFIG_DIRS/uv/uv.toml`) on macOS and Linux, or
+`%SYSTEMDRIVE%\ProgramData\uv\uv.toml` on Windows.
+
+User-, venv-, and system-level configuration must use the `uv.toml` format, rather than the
+`pyproject.toml` format, as a `pyproject.toml` is intended to define a Python _project_.
+
+If project-, venv-, user-, and system-level configuration files are found, the settings will be
+merged, with project-level configuration taking precedence over venv-level configuration,
+venv-level configuration taking precedence over user-level configuration, and user-level
+configuration taking precedence over system-level configuration. (If multiple system-level
 configuration files are found, e.g., at both `/etc/uv/uv.toml` and `$XDG_CONFIG_DIRS/uv/uv.toml`,
 only the first-discovered file will be used, with XDG taking priority.)
 
-For example, if a string, number, or boolean is present in both the project- and user-level
-configuration tables, the project-level value will be used, and the user-level value will be
+For example, if a string, number, or boolean is present in both the project- and venv-level
+configuration tables, the project-level value will be used, and the venv-level value will be
 ignored. If an array is present in both tables, the arrays will be concatenated, with the
 project-level settings appearing earlier in the merged array.