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Trouble running example 2 - Tree of Life #120

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sierra-smith opened this issue Dec 1, 2023 · 0 comments
Open

Trouble running example 2 - Tree of Life #120

sierra-smith opened this issue Dec 1, 2023 · 0 comments

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@sierra-smith
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Hello!

I am attempting to run example 2 in order to recreate the microbial tree of life and I am having trouble downloading the reference genomes.

First, I was having trouble downloading the assembly_summary_genbank.txt file remotely so I manually downloaded it and placed it in my environment.

Then, I tried to rerun phylophlan_get_reference
-g all
-o input_genomes/
-n 1
--verbose 2>&1 | tee logs/phylophlan_get_reference.log

And received the error message:

File "taxa2genomes.txt" present
File "taxa2genomes_cpa201901_up201901.txt.bz2" present
Output folder "input_genomes/" present
File "assembly_summary_genbank.txt" present
Downloading 1 reference genomes for k__Bacteria|p__Actinobacteria|c__Actinobacteria|o__Actinomycetales|f__Actinomycetaceae|g__Actinomyces|s__Actinomyces_radingae
Downloading "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/106/055/GCA_900106055.1_IMG-taxon_2630968294_annotated_assembly/GCA_900106055.1_IMG-taxon_2630968294_annotated_assembly_genomic.fna.gz" to "input_genomes/GCA_900106055.fna.gz"
[e] unable to download "https://ftp.ncbi.nlm.nih.gov/genomes/all/GCA/900/106/055/GCA_900106055.1_IMG-taxon_2630968294_annotated_assembly/GCA_900106055.1_IMG-taxon_2630968294_annotated_assembly_genomic.fna.gz"

Can you please let me know what may be causing the problem? I am running this on MacOS Ventura 13.4.

Thank you!
Sierra

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