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incorrect value for phred_offset in qiime #2215

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hallerliran opened this issue Jul 29, 2018 · 1 comment
Open

incorrect value for phred_offset in qiime #2215

hallerliran opened this issue Jul 29, 2018 · 1 comment

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@hallerliran
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hallerliran commented Jul 29, 2018

During a quality filtering step of joined reads I keep getting an error message about phred_offset.

I am running the multiple_split_libraries_fastq.py command.

error:

skbio.parse.sequences._exception.FastqParseError: Failed qual conversion for seq id: M04464:129:000000000-BTGC6:1:1101:13468:1587 1:N:0:TCATGCGCGA. This may be because you passed an incorrect value for phred_offset.

I tried manually specifying phred_offsert values (either 33 or 64) in the info.txt file but it didn't work. Have someone encountered this issue before?

Thanks!

@songzhang-master
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Hello, I have encountered the same problem. Can you solve it?

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