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---
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title : " Day 1 Recap"
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- author : " "
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- date : " 21/09/2022 "
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+ author : ' '
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+ date : " 05/12/2024 "
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output :
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+ beamer_presentation : default
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ioslides_presentation :
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widescreen : true
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smaller : true
@@ -24,7 +25,7 @@ knitr::include_graphics('Images/workflow2.png')
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* cells: Bone Marrow Mononuclear cells (BMMCs)
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* 12 samples
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* 4 Sample groups
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- * HHD: The high hyper diploid cases.
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+ * HHD: The high hyper diploid cases (51–67 chromosomes) .
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* Two replicates.
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* PBMMC: healthy pediatric BMMC.
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* Four replicates.
@@ -47,16 +48,20 @@ for each "read".
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* ** UMI** - identifies the transcript molecule within a cell and gene
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* ** insert** - the transcript molecule
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+
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``` {r echo=FALSE, out.width='60%', fig.align='center'}
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knitr::include_graphics('Images/tenxLibStructureV3dual2.png')
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```
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+
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+
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## Cell Ranger
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* 10x Cell Ranger - This not only carries out the alignment and feature
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counting, but will also:
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* Call cells
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+ * Generates counts matrix
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* Generate a summary report in html format
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* Generate a "cloupe" file
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@@ -82,7 +87,7 @@ cellranger count --id={OUTPUT_SAMPLE_NAME} \
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The contents of the ` outs ` directory are:
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- ``` {r outputDirOuts, fig.align='center', out.width="50 %"}
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+ ``` {r outputDirOuts, echo=FALSE, fig.align='center', out.width="75 %"}
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knitr::include_graphics("Images/CellRangerOutputOuts.png")
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```
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