Consider single experiment per temperature approach

[bjartelund]

On the estimation errors of KM and V from time-course experiments using the Michaelis–Menten equation
Wylie Stroberg, Santiago Schnell
bioRxiv 068015; doi: https://doi.org/10.1101/068015
Now published in Biophysical Chemistry doi: 10.1016/j.bpc.2016.09.004

[bjartelund]

Some clear drawbacks with the approach:
You would have to run the individual experiments much longer
In my case, you reach a saturation-point where the detector is no longer capable of measuring the signal.

[bjartelund]

thermofit-gradient does use the absorbance to calculate concentration, could be extended to the other script as well