Error in stage5

[LiJIAJUN-G]

In the docker image, I managed to run most of the first, but there was an error in stage5.
Start stage 5: neoantigen filtering using Pre&RecNeo model and refined immunogenicity score scheme.
Process Process-11:
Traceback (most recent call last):
File "/usr/lib/python2.7/multiprocessing/process.py", line 258, in _bootstrap
self.run()
File "/usr/lib/python2.7/multiprocessing/process.py", line 114, in run
self._target(*self._args, **self._kwargs)
File "/root/pTuneos/src/core/pairendMDNA/pairendMDNAprocessor.py", line 471, in InVivoModelAndScore
homolog_s=cal_similarity_per(M_P,H_P)
File "/root/pTuneos/src/core/pairendMDNA/pairendMDNAprocessor.py", line 356, in cal_similarity_per
score_pair=aligner(mut_seq,normal_seq)[0][2]
File "/root/pTuneos/src/core/pairendMDNA/pairendMDNAprocessor.py", line 329, in aligner
aln = pairwise2.align.localds(seq1.upper(), seq2.upper(), matrix,gap_open,gap_extend)
File "/usr/local/lib/python2.7/dist-packages/Bio/pairwise2.py", line 408, in call
return _align(**keywds)
File "/usr/local/lib/python2.7/dist-packages/Bio/pairwise2.py", line 462, in _align
align_globally, score_only)
File "/usr/local/lib/python2.7/dist-packages/Bio/pairwise2.py", line 1031, in call
return self.score_dict[(charA, charB)]
KeyError: ('-', 'E')