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Homology arm mapping #11

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fheigwer opened this issue Feb 20, 2017 · 0 comments
Open

Homology arm mapping #11

fheigwer opened this issue Feb 20, 2017 · 0 comments

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@fheigwer
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retrieve 3’ and 5’ homology sequence retrieves sequence up and downstream of the spCAS9 break point (17th spacer base pair). This is not compliant with other PAMs, especially for Cpf1. It should be changed to: left sequence, PAM+spacer, right sequence
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@fheigwer