From 7b1e2c8f09ddc1c84e0c4168bf93949558f66749 Mon Sep 17 00:00:00 2001 From: albeez Date: Fri, 10 Jan 2025 12:46:01 -0500 Subject: [PATCH] Update README.md --- README.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/README.md b/README.md index 1706cf3..1a7fe5f 100644 --- a/README.md +++ b/README.md @@ -158,6 +158,9 @@ Each plate of samples that you run in CARMEN must include the following 3 contro 3. Positive control must contain the phrase ``"CPC"``. * The ``"Combined Positive Control"`` contains a combined assay of synthetic targets corresponding to all viral assays tested in your CARMEN run. * A positive signal for the CPC per viral assay is integral to validating the viral assay in your CARMEN run. + 4. Negative Viral Assay Control must contain the phrase ``no_crRNA``. + * The ``no_crRNA`` control does not contain any viral crRNA assay and each sample should test negative against this assay. + * A negative signal for the ``no_crRNA`` control is integral to validating the sample tested in your CARMEN run. The assumption for the remainder of the CARMEN analysis is that any sample that does contain the phrases ``NTC``, ``NDC``, and ``CPC`` is considered a “patient or clinical sample”.