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v5.4.1
Resolved bug in saving QC scores to csv
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analyze_run.py

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@@ -121,7 +121,7 @@
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## Set up structure of the output folder - simplify into RESUTLS, QUALITY CONTROL, R&D
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# make an output folder in your path's wd if it hasn't been made already
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output_folder = f'output_{barcode_assignment}_[{CLI_arg[1]}]_v{software_version}'
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output_folder = f'output_{barcode_assignment}_{CLI_arg[1]}_v{software_version}'
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if not os.path.exists(output_folder):
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os.makedirs(output_folder)
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threshold.py

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@@ -87,7 +87,6 @@ def raw_thresholder(self, unique_crRNA_assays, assigned_only, t13_df, CLI_thresh
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norm_thresholds_df = raw_thresholds_df.loc['NTC Threshold'] / ntc_mean_df
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raw_thresholds_df = pd.concat([raw_thresholds_df, norm_thresholds_df], ignore_index=True, axis=0)
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raw_thresholds_df.index = ['NTC Mean', 'NTC Standard Deviation', 'NTC 3*SD', 'NTC Threshold', 'Normalized NTC Threshold']
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else:
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print("Consult ReadME and input appropriate command-line arguments to specify thresholding method.")

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