Figure 2 Discussion

[gwaybio]

Figure 2. Comparing Cell Painting and L1000 readouts of compound perturbations by mechanism of action (MOA). (a) Median replicate correlation of compounds measured in both assays compared across doses. The color of the point represents how often the compound perturbation is reproducible across doses and assays. The maximum count is 12 (6 doses x 2 assays). (b) Median correlation between compounds annotated to the same MOA. We derived the null threshold through a nonparametric permutation test of randomly sampled compounds (see methods). The size of the points represent how many compounds belong to the MOA class. (c) All 31 MOAs that are consistently measured by either Cell Painting or L1000 in three or more doses. The larger the text, the more reproducible doses per MOA.