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Bin refinement: empty binsO #554

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ereyred opened this issue Sep 10, 2024 · 0 comments
Open

Bin refinement: empty binsO #554

ereyred opened this issue Sep 10, 2024 · 0 comments

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@ereyred
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ereyred commented Sep 10, 2024

Hello,
I've run metawrap with the three default binners and -l 1500. Now trying to do bin_refinement (-c 50 -x 10) but it tells me:


----- Scanning to find duplicate contigs between bins and only keep them in the best bin... -----

Loading in bin completion and contamination scores...
Loading in contigs in each bin...
Making a new dereplicated version of each bin file


----- You will find the best non-reassembled versions of the bins in binsO -----

########################################################################################################################

FINALIZING THE REFINED BINS

########################################################################################################################


----- Re-running CheckM on binsO bins -----


[CheckM - tree] Placing bins in reference genome tree.


[Error] No bins found. Check the extension (-x) used to identify bins.

Controlled exit resulting from an unrecoverable error or warning.


***** Something went wrong with running CheckM. Exiting... *****


I've seen in other people's Issues that when they got this error their binsAB/binsBC/binsAC were empty. Mine are not empty. My binsO (and binsO.tmp is still there and empty as well) is empty though, even though that step gave no error. Why did "Making a new dereplicated version of each bin file" remove everything??

Thanks for your help!
E

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