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C elegans subject metadata #171
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for c elegans: see supplemental file 7 here: https://www.biorxiv.org/content/10.1101/2020.04.30.066209v3.supplementary-material also i think it would be good to rethink the nwb core schemas around subject. if you are going to redo pieces of the score schema, let's do a better job of bringing many of the elements in dandischema and from the aind work into the core schema. @saskiad and i also looked at a bunch of related things. finally, more work is coming from cell lines and organoids. we are also planning on modeling all of this in linkml relatively soon (a lot has already happened connected to this), which may offer a good route for many downstream tasks. |
From follow-up emails with @dysprague et al, they will be creating a
This captures the stages described in supp file 7, except that the paper authors describe estimated hours since birth/hatch rather than time since the start of a growth stage. I am not a C elegans expert, so do not know which is more common or useful... Growth stages that @dysprague et al identified for neurophysiology are:
|
The NWB team is also looking at this. Let's discuss at the next sync. |
@rly - your proposal looks reasonable to me. the only change i would make is allowing for a range of durations in addition to duration (200 - 400 mins). we crafted a patch to ISO8601 to encode such a range in dandi, as is often needed in certain experiments where a more precise time is not available. one consideration is whether there should be a specific class for a specific species, or a more general concept. finally, the concept of age here is related to a concept of environment (which presently seems to be temperature, but one may easily consider other parameters). thus the model could separate those considerations. it also seems that the stage identifier does specify temperature. |
We are trying to help a group get C. elegans data into NWB and DANDI and they are coming up against some incompatibilities in the subject metadata.
From a conversation with @rly and @dysprague:
Subject.sex
is currently limited to "M", "F", "U", or "O". For C. elegans, would it be possible to also accept "XO" (male) and "XX" (hermaphrodite)? Would it be possible to accept such data in the NWB schema?Subject.age
. For C. elegans, they are not sure if they can recover the age of the worm. It would be more informative to store the growth stage. We could extendSubject
to a new ndtypeCElegansSubject
that has an additional fieldgrowth_stage
, and make the necessary changes in NWB Inspector. Would it be possible to accept such data in the NWB schema?We have started a Draft PR to make these changes for (1) in the NWB Inspector: NeurodataWithoutBorders/nwbinspector#353
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