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dandiset.yaml
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# DO NOT EDIT THIS FILE LOCALLY. ALL LOCAL UPDATES WILL BE LOST.
# It can be edited online at https://dandiarchive.org/dandiset/000026
# and obtained from the dandiarchive.
'@context': https://raw.githubusercontent.com/dandi/schema/master/releases/0.6.0/context.json
about:
- name: Broca’s Area
schemaKey: GenericType
- name: Motor Cortex
schemaKey: GenericType
access:
- contactPoint:
schemaKey: ContactPoint
schemaKey: AccessRequirements
status: dandi:OpenAccess
assetsSummary:
approach: []
dataStandard:
- identifier: RRID:SCR_016124
name: Brain Imaging Data Structure (BIDS)
schemaKey: StandardsType
measurementTechnique: []
numberOfBytes: 38278696320795
numberOfFiles: 40982
numberOfSamples: 62
numberOfSubjects: 20
schemaKey: AssetsSummary
species: []
variableMeasured: []
citation: Mazzamuto, Giacomo; Costantini, Irene; Danila Di Meo; Castelli, Filippo
Maria; Pesce, Luca; Scardigli, Marina; Pavone, Francesco Saverio; Roffilli, Matteo;
Silvestri, Ludovico; Brady, Niamh; Ramazzotti, Josephine; Hof, Patrick R.; Boas,
David A.; Fischl, Bruce; Morgan, Leah; Yang, Jiarui; Chang, Shuaibin; Laffey, Jessie;
Magnain, Caroline; Varadarajan, Divya; Wang, Hui; Frost, Robert; Kouwe, Andre van
der; Player, Allison Stevens; Atzeni, Alessia; Gonzalez, Juan Eugenio Iglesias;
Balbastre, Yael; Vera, Matthew; Cordero, Devani; Nestor, Kimberly; Ammon, William;
Nolan, Jackson; Mora, Jocelyn; Pallares, Erendira Garcia; Augustinack, Jean; Diamond,
Bram; Fogarty, Morgan; Boyd, Emma; Varghese, Merina; Dalca, Adrian V.; Edlow, Brian;
Frosche, Matthew; Wicinski, Bridget; Chen, I-Chun Anderson; Gavryusev, Vladislav
(2024) Human brain cell census for BA 44/45 (Version draft) [Data set]. DANDI archive.
https://dandiarchive.org/dandiset/000026/draft
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dateCreated: '2020-06-24T18:34:50.022000+00:00'
description: Magnetic resonance imaging (MRI) is used to establish a macroscopic reference
coordinate system of laminar and cytoarchitectural boundaries. Cell counting is
obtained with both traditional immunohistochemistry, to provide a stereological
gold standard, and with a custom-made inverted confocal light-sheet fluorescence
microscope (LSM) for 3D imaging at cellular resolution. Finally, polarization-sensitive
optical coherence tomography (PSOCT) enables registration of the distorted histological
cell typing obtained with LSM to the MRI-based atlas coordinate system.
ethicsApproval: []
id: DANDI:000026/draft
identifier: DANDI:000026
keywords:
- multi-modal imaging
- MRI
- OCT
- SPIM
- human cortex
- Broca's area
- Motor cortex
- Stereology
license:
- spdx:CC-BY-4.0
manifestLocation:
- https://api.dandiarchive.org/api/dandisets/000026/versions/draft/assets/
name: Human brain cell census for BA 44/45
protocol:
- https://www.protocols.io/view/high-resolution-ex-vivo-structural-mri-imaging-amp-byt7pwrn
- https://www.protocols.io/view/serial-sectioning-optical-histology-cell-census-ne-bu7mnzk6
- https://www.biorxiv.org/content/10.1101/2021.07.18.452829v1
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name: 'Autofluorescence enhancement for label-free imaging of myelinated fibers
in mammalian brains '
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repository: Scientific Reports
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url: https://www.biorxiv.org/content/10.1101/2020.07.28.225011v1
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name: Dual-beam confocal light-sheet microscopy via flexible acousto-optic deflector
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repository: J. of Biomedical Optics
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name: Improving the characterization of ex vivo human brain optical properties using
high numerical aperture optical coherence tomography by spatially constraining
the confocal parameters
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repository: Neurophotonics
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url: https://pubmed.ncbi.nlm.nih.gov/33094126/
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name: 'as-PSOCT: Volumetric microscopic imaging of human brain architecture and
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name: Measuring myelin content and cell density in human brain with Optical Coherence
Tomography
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name: Fast volumetric mapping of human brain slices
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name: A novel algorithm to optimize generalized gamma distributed multiplicative
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of human brain cytoarchitecture (MAGIC: A label-free fluorescence method for 3D
high-resolution reconstruction of myelinated fibers in large volumes)'
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repository: Biomedical Optics Express
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7T ex vivo MRI of the human cerebral cortex
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studyTarget:
- 'Our consortium will develop and utilize an imaging infrastructure to create a human
brain cell census and instantiate it in a coordinate system that will enable an
immediate impact of all in vivo MRI studies of the human brain. Our imaging pipeline
begins with 150 μm isotropic ex vivo structural MRIs and 500 μm 90 direction diffusion
MRI. 4x4x2 cm tissue blocks will then be imaged with serial sectioning polarization
sensitive optical coherence tomography (PS-OCT) to obtain 20 μm isotropic resolution
images of cyto- and myelo-architectural features and fiber tractography. Registration
with the MRI is preserved because imaging is performed before sectioning. Serial
sections with 150 μm thickness are cut from the block face, cleared, and subsequently
imaged by selective plane imaging microscopy (SPIM) with 1 μm isotropic resolution,
and with multiplexed histological staining of multiple molecular markers. Segmentation
of common features amongst the SPIM, OCT, and MRI, permits registration of the cellular
information from SPIM back to the MRI coordinate system with OCT serving as an important
intermediary permitting registration of SPIM with MRI with micrometer resolution.
Our aims are designed to establish our imaging workflow and build a cell census
atlas for language regions in human frontal cortex, resulting in a clear path forward
for obtaining the cell census for the entire human brain. '
url: https://dandiarchive.org/dandiset/000026/draft
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