Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Please increase the window size. Coverage is too low! error #351

Open
rezenman opened this issue Aug 23, 2023 · 0 comments
Open

Please increase the window size. Coverage is too low! error #351

rezenman opened this issue Aug 23, 2023 · 0 comments

Comments

@rezenman
Copy link

Hey all,
I am working with a genome of the yeast Saccharomyces cerevisiae, it is a specific strain so I need to build my own mappability map.
I followed the pipeline in the faq and everything looks well but when running the following command:
delly cnv -a -y 1 -g $ref -m $map $bam_file > cnv.vcf
I get the error:
Please increase the window size. Coverage is too low!
I tried modifying the parameters: -ijw but it didn't work for any number up to 1000000
I know that the coverage in my bam file is good, and I think the mappability map also make sense, just to make sure I counted A,C and N bases in my contigs:

NC_001133: Size=230225 A=10255 N=21551 C=198419 G=0 T=0
NC_001134: Size=813166 A=6527 N=29442 C=777197 G=0 T=0
NC_001135: Size=316615 A=4805 N=18115 C=293695 G=0 T=0
NC_001136: Size=1532214 A=18763 N=84345 C=1429106 G=0 T=0
NC_001137: Size=575776 A=3781 N=26610 C=545385 G=0 T=0
NC_001138: Size=270165 A=3542 N=13827 C=252796 G=0 T=0
NC_001139: Size=1090945 A=6778 N=44766 C=1039401 G=0 T=0
NC_001140: Size=562642 A=12664 N=33545 C=516433 G=0 T=0
NC_001141: Size=439890 A=9366 N=20179 C=410345 G=0 T=0
NC_001142: Size=745757 A=15890 N=42467 C=687400 G=0 T=0
NC_001143: Size=666817 A=2487 N=5650 C=658680 G=0 T=0
NC_001144: Size=1075784 A=19062 N=80427 C=976295 G=0 T=0
NC_001145: Size=924434 A=4877 N=36268 C=883289 G=0 T=0
NC_001146: Size=784503 A=8410 N=33207 C=742886 G=0 T=0
NC_001147: Size=1091098 A=9315 N=40339 C=1041444 G=0 T=0
NC_001148: Size=948056 A=9286 N=49074 C=889696 G=0 T=0
NC_001224: Size=85779 A=83 N=710 C=84986 G=0 T=0

Any help will be appreciated!
Shahar

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant