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I have assembled a metatranscriptome and wonder now if gene calling for downstream analysis is necessary or not. The contigs should be ORFs anyways as they are transcribed right? However, when I do run prodigal on the contigs I get slighly lower number of ORFs than the contig number. Does that makes sense to you?
Many thanks for any thought and advice!
The text was updated successfully, but these errors were encountered:
Hi,
I have assembled a metatranscriptome and wonder now if gene calling for downstream analysis is necessary or not. The contigs should be ORFs anyways as they are transcribed right? However, when I do run prodigal on the contigs I get slighly lower number of ORFs than the contig number. Does that makes sense to you?
Many thanks for any thought and advice!
The text was updated successfully, but these errors were encountered: