non-targeting controls?

[AmandaKedaigle]

Thanks - this looks nice and is so refreshingly easy to install and use! I was wondering if there was a way in CB2 to account for non-targeting control sgRNAs, or if this is not needed? Thanks

[hyunhwan-jeong]

Hello @AmandaKedaigle,

The current version CB² does not have any data correction function using non-targeting control in the latest version of You can look up the p-values of each individual non-targeting sgRNA for the data quality control and I would suggest getting rid of them in your gene-level analysis.

Please share your thought if you want to add some functions in CB² regarding the matters!

Best Regards,

Hyun-Hwan Jeong