Changes:
| version | modifications |
|---|---|
| v1.1.0 | + added strandedness information for single exon genes |
| + fixed GUI rules for CRISPRa/i | |
| + added option so TSS are only counted if belonging to the gene of interest | |
| + changed bowtie off-target mode to -k ( offtargets allowed +1 ) so that bowtie always searches if designs have less or at least on off-target more than allowed. This way, sgRNAs are excluded which do have more than the allowed off-targets and included if less. Earlier one could allow more off-targets then bowtie was searching for. | |
| v1.2.0 | + fixed overlapping criterion to beeing 3 bp downstream of the PAM (sgRNAend - 5bp) |
| v1.3.0 | + fixed error in version 1.2 where filter criteria like exons were not interpreted |
| + enabled automated deletion of single gene files in the server appliation so only the usmmary is kept | |
| + fixed all binary builds | |
| v1.3.1 | + fixed error in version 1.3.0 where gene names with underscores caused major trouble |