| Column name | description |
|---|---|
| Name | target site ID |
| Length | target length |
| Start | target start with respect to the input sequence (gene -500 if a gene name was the input) |
| End | target start with respect to the input sequence (gene -500 if a gene name was the input) |
| Strand | strand it will target |
| Nucleotide sequence | target site nucleotide composition of the form target_PAM |
| Gene Name | ID::GENE |
| Transcripts | ENSEMBL transcript Ids overlapping with the target site |
| Transcript:: Exon | ENSEMBL transcript::exon Ids overlapping with the target site |
| Number of Cpg Islands hit | Number of Cpg Islands overlapping with the target site |
| Sequence around the cutside | if it is chosen to save a recombination matrix ist sequence is here |
| %A %C %T %G | nucleotide compositions in per cent |
| S-Score | specificity score |
| A-Score | annotation score |
| Custom-Score | by default the score from Doench et al. 2014 else the score deviated from the custom Perl scoring script |
| percent of total transcripts hit | per cent of transcripts of the targeted gene being hit by that putative sgRNA |
| Target | target genes by remapping the target site |
| Match-start | alignment start with respect to the estimate target gene |
| Match-end | alignment end with respect to the estimate target gene |
| Matchstring | alignment representation "M" for match "X" for mismatch "I" for insertion "D" for deletion |
| Editdistance | estimated edit distance of the alignment (X+I+D) |
| Number of Hits | estimate number of target sites in the respective genome with the off-target parameters specified |
| Direction | strandedness of the target alignment |
| Doench-Score | Efficacy score as introduced by Doench et al. 2014 Nat. Biotech. |
| Xu-Score | Efficacy score as introduced by Xu et al. 2015 Gen.Res. |