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usage.txt
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usage.txt
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__ __ _____ _____ ______ _ _ ______
| \\/ | | __ \\ / ____| ____| \\ | | ____|
| \\ / | ___| |__) |__ _| | __| |__ | \\| | |__
| |\\/| |/ _ \\ _ // _` | | |_ | __| | . ` | __|
| | | | __/ | \\ \\ (_| | |__| | |____| |\\ | |____
|_| |_|\\___|_| \\_\\__,_|\\_____|______|_| \\_|______|
USAGE
nextflow run metagenomics/MeRaGENE [OPTIONAL_ARGUMENTS] (--genome --ncbi --input --output --cov )
DESCRIPTION
MeRaGENE 0.1.0
--genome="/vol/genomeDat/test.faa" Your faa to search in.
--ncbi="/vol/blastDat/blast.db" Your blast-database to search in.
--input="/vol/project/hmmModels" A folder containing hmm models. All hmm models in this folder are used for searching.
--output="/vol/project/output" A folder path that the pipeline should produce.
--cov="/vol/cov1.txt,/vol/cov2.txt" List your coverage files, link them here.
OPTIONAL ARGUMENTS
--blast_cpu=8 Numbers of cores to be used executing blast.
--blastp="blastp"
--hmm_search="hmmsearch"
--hmm_scan="hmmscan"
--hmm_press="hmmpress" If you want to use a special version, change the name with its absolute path.
E.g. blastp="blastp" -> blastp="/vol/tools/blast/blastp"
--hmm_cpu=16 Numbers of cores to be used executing hmmsearch.
--hmm_evalue="1e-15" E-value threshold to be used executing hmmsearch.
--search="/vol/project/search.yaml" If you want your results grouped, group them using a first level .yaml file.
If you have downloaded MeRaGENE, you can look at the example file features/data/search.yaml
--keywords="/vol/project/keywords.txt" A text file, filled with one word per line.
Publications associated with your blast hits will be scanned for these keywords.