From 784acbbda20fca0c9f4647bd8dad21c8a11e631e Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 27 Sep 2023 06:26:51 +0000 Subject: [PATCH 01/89] Added target capture simulation and modified amplicon simulation --- CHANGELOG.md | 1 + assets/schema_input.json | 27 +++------ conf/modules.config | 56 +++++++++++++++++-- main.nf | 4 +- modules.json | 21 +++++++ nextflow.config | 16 +++++- nextflow_schema.json | 39 ++++++++++++- workflows/readsimulator.nf | 109 ++++++++++++++++++++++++++----------- 8 files changed, 213 insertions(+), 60 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ec9fbd7..0e7b4cb 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,6 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 Initial release of nf-core/readsimulator, created with the [nf-core](https://nf-co.re/) template. ### `Added` +Subworkflow to simulate illumina amplicon data ### `Fixed` diff --git a/assets/schema_input.json b/assets/schema_input.json index 51606d1..90d0551 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,27 +10,16 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "unique": true, + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] }, - "fastq_1": { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$", - "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" - }, - "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] + "seed": { + "errorMessage": "Seed must be provided", + "unique": true, + "meta": ["seed"] } }, - "required": ["sample", "fastq_1"] + "required": ["sample", "seed"] } } diff --git a/conf/modules.config b/conf/modules.config index da58a5d..8aaac49 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,14 +18,62 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: SAMPLESHEET_CHECK { + withName: CRABS_DBIMPORT { + ext.args = "--seq_header accession --delim ' '" publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + path: { "${params.outdir}/crabs_dbimport" }, + mode: params.publish_dir_mode + ] + } + + withName: CRABS_INSILICOPCR { + ext.args = "--error 4.5 -t 12 --fwd ${params.fw_primer} --rev ${params.rv_primer}" + publishDir = [ + path: { "${params.outdir}/crabs_insilicopcr" }, + mode: params.publish_dir_mode ] } + withName: ART_ILLUMINA { + ext.args = "-amp -p -na -c 500" + publishDir = [ + path: { "${params.outdir}/art_illumina" }, + mode: params.publish_dir_mode + ] + } + + withName: BOWTIE2_ALIGN { + ext.args = "--local --very-sensitive-local --mp 32 --rdg 10,8 --rfg 10,8 -k 10000 -f" + ext.args2 = "-b | samtools sort" + publishDir = [ + path: { "${params.outdir}/bowtie2" }, + mode: params.publish_dir_mode + ] + } + + withName: BOWTIE2_BUILD { + publishDir = [ + path: { "${params.outdir}/bowtie2" }, + mode: params.publish_dir_mode + ] + } + + withName: PBSIM { + ext.args = "--model_qc" + publishDir = [ + path: { "${params.outdir}/pbsim" }, + mode: params.publish_dir_mode + ] + } + + //withName: SAMPLESHEET_CHECK { + // publishDir = [ + // path: { "${params.outdir}/pipeline_info" }, + // mode: params.publish_dir_mode, + // saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + // ] + //} + withName: FASTQC { ext.args = '--quiet' } diff --git a/main.nf b/main.nf index f050787..74c418b 100644 --- a/main.nf +++ b/main.nf @@ -17,7 +17,9 @@ nextflow.enable.dsl = 2 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +if (!params.fasta) { + params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ diff --git a/modules.json b/modules.json index 039aea8..330aba5 100644 --- a/modules.json +++ b/modules.json @@ -5,6 +5,22 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { + "art/illumina": { + "branch": "master", + "git_sha": "240937a2a9c30298110753292be041188891f2cb", + "installed_by": ["modules"], + "patch": "modules/nf-core/art/illumina/art-illumina.diff" + }, + "bowtie2/align": { + "branch": "master", + "git_sha": "fe54581f8bed20e4c4a51c616c93fd3379d89820", + "installed_by": ["modules"] + }, + "bowtie2/build": { + "branch": "master", + "git_sha": "6a24fbe314bb2e6fe6306c29a63076ea87e8eb3c", + "installed_by": ["modules"] + }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "76cc4938c1f6ea5c7d83fed1eeffc146787f9543", @@ -19,6 +35,11 @@ "branch": "master", "git_sha": "f2d63bd5b68925f98f572eed70993d205cc694b7", "installed_by": ["modules"] + }, + "samtools/index": { + "branch": "master", + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": ["modules"] } } } diff --git a/nextflow.config b/nextflow.config index 8f89683..1834db1 100644 --- a/nextflow.config +++ b/nextflow.config @@ -14,9 +14,19 @@ params { input = null // References genome = null - igenomes_base = 's3://ngi-igenomes/igenomes' + //igenomes_base = 's3://ngi-igenomes/igenomes' + igenomes_base = null igenomes_ignore = false - + + fasta = null + //seeds = null + //prefix = null + fw_primer = 'GTCGGTAAAACTCGTGCCAGC' + rv_primer = 'CATAGTGGGGTATCTAATCCCAGTTTG' + + // Simulation options + //illumina_amplicon = null + //illumina_uce = null // MultiQC options multiqc_config = null @@ -43,7 +53,7 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - + // Max resource options // Defaults only, expecting to be overwritten diff --git a/nextflow_schema.json b/nextflow_schema.json index e2112bb..39502b2 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -20,7 +20,8 @@ "pattern": "^\\S+\\.csv$", "description": "Path to comma-separated file containing information about the samples in the experiment.", "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/readsimulator/usage#samplesheet-input).", - "fa_icon": "fas fa-file-csv" + "fa_icon": "fas fa-file-csv", + "schema": "assets/schema_input.json" }, "outdir": { "type": "string", @@ -42,6 +43,42 @@ } } }, + "simulation_options": { + "title": "Simulation options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Choose the data types that should be simulated by the pipeline.", + "properties": { + "illumina_amplicon": { + "type": "boolean", + "description": "Option to simulate Illumina amplicon data.", + "fa_icon": "fas fa-question-circle" + }, + "illumina_uce": { + "type": "boolean", + "description": "Option to simulate Illumina UCE data.", + "fa_icon": "fas fa-question-circle" + } + } + }, + "amplicon_options": { + "title": "Amplicon options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Options required when simulating amplicon data.", + "properties": { + "fw_primer": { + "type": "string", + "description": "Forward primer to use with crabs_insilicopcr. E.g., GTCGGTAAAACTCGTGCCAGC", + "default": "GTCGGTAAAACTCGTGCCAGC" + }, + "rv_primer": { + "type": "string", + "description": "Reverse primer to use with crabs_insilicopcr. E.g., CATAGTGGGGTATCTAATCCCAGTTTG", + "default": "CATAGTGGGGTATCTAATCCCAGTTTG" + } + } + }, "reference_genome_options": { "title": "Reference genome options", "type": "object", diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 8a2b8b3..540c29f 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -4,7 +4,7 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryLog; paramsSummaryMap; fromSamplesheet } from 'plugin/nf-validation' def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) def citation = '\n' + WorkflowMain.citation(workflow) + '\n' @@ -35,7 +35,8 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { INPUT_CHECK } from '../subworkflows/local/input_check' +include { ILLUMINA_AMPLICON } from '../subworkflows/local/illumina_amplicon' +include { ILLUMINA_UCE } from '../subworkflows/local/illumina_uce' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -62,25 +63,69 @@ def multiqc_report = [] workflow READSIMULATOR { ch_versions = Channel.empty() + ch_fasta = Channel.fromPath(params.fasta) + //ch_fasta = ch_fasta.map { + // fasta -> + // def meta = [:] + // meta.id = params.prefix + // [ meta, fasta ] + //} + //ls_seeds = params.seeds?.tokenize(',') + //ch_seed = Channel.fromList(ls_seeds) // // SUBWORKFLOW: Read in samplesheet, validate and stage input files // - INPUT_CHECK ( - file(params.input) - ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema + //INPUT_CHECK ( + // file(params.input) + //) + //ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) + + ch_input = Channel.fromSamplesheet("input") + // .map { meta -> + // def fasta = file(params.fasta) + // return [ meta[0], fasta ] + // } + + // + // SUBWORKFLOW: Simulate illumina amplicon reads + // + if ( params.illumina_amplicon ) { + ILLUMINA_AMPLICON ( + ch_fasta, + ch_input + ) + ch_versions = ch_versions.mix(ILLUMINA_AMPLICON.out.versions.first()) + } + + // + // SUBWORKFLOW: Simulate illumina UCE reads + // + if ( params.illumina_uce ) { + ch_probes = Channel.fromPath(params.probes) + ch_probes = ch_probes.map { + fasta -> + def meta = [:] + meta.id = "probes" + [ meta, fasta ] + } + ILLUMINA_UCE ( + ch_fasta, + ch_probes + ) + ch_versions = ch_versions.mix(ILLUMINA_UCE.out.versions.first()) + } + + // MODULE: Create sample sheet + // I plan on having a module here that creates a samplesheet that can be used with other nf-core pipelines // // MODULE: Run FastQC // - FASTQC ( - INPUT_CHECK.out.reads - ) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + //FASTQC ( + // ILLUMINA_AMPLICON.out.reads + //) + //ch_versions = ch_versions.mix(FASTQC.out.versions.first()) CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') @@ -89,25 +134,25 @@ workflow READSIMULATOR { // // MODULE: MultiQC // - workflow_summary = WorkflowReadsimulator.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowReadsimulator.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - - MULTIQC ( - ch_multiqc_files.collect(), - ch_multiqc_config.toList(), - ch_multiqc_custom_config.toList(), - ch_multiqc_logo.toList() - ) - multiqc_report = MULTIQC.out.report.toList() + //workflow_summary = WorkflowReadsimulator.paramsSummaryMultiqc(workflow, summary_params) + //ch_workflow_summary = Channel.value(workflow_summary) + + //methods_description = WorkflowReadsimulator.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) + //ch_methods_description = Channel.value(methods_description) + + //ch_multiqc_files = Channel.empty() + //ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + //ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) + //ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) + //ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + + //MULTIQC ( + // ch_multiqc_files.collect(), + // ch_multiqc_config.toList(), + // ch_multiqc_custom_config.toList(), + // ch_multiqc_logo.toList() + //) + //multiqc_report = MULTIQC.out.report.toList() } /* From 9c1ccafce281ac82a13b725009a7533a27a52cf7 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 27 Sep 2023 10:10:44 +0000 Subject: [PATCH 02/89] added amplicon illumina + target capture illumina and pacbio --- conf/modules.config | 18 +++ modules/local/capsim.nf | 42 +++++++ modules/local/crabs_dbimport.nf | 37 ++++++ modules/local/crabs_insilicopcr.nf | 37 ++++++ modules/local/pbsim.nf | 41 +++++++ modules/local/simlord.nf | 38 +++++++ .../nf-core/art/illumina/art-illumina.diff | 37 ++++++ modules/nf-core/art/illumina/main.nf | 71 ++++++++++++ modules/nf-core/art/illumina/meta.yml | 59 ++++++++++ modules/nf-core/bowtie2/align/main.nf | 94 ++++++++++++++++ modules/nf-core/bowtie2/align/meta.yml | 67 +++++++++++ modules/nf-core/bowtie2/build/main.nf | 42 +++++++ modules/nf-core/bowtie2/build/meta.yml | 43 +++++++ modules/nf-core/samtools/index/main.nf | 48 ++++++++ modules/nf-core/samtools/index/meta.yml | 53 +++++++++ nextflow.config | 15 ++- nextflow_schema.json | 31 +++++- subworkflows/local/amplicon.nf | 90 +++++++++++++++ subworkflows/local/target_capture.nf | 105 ++++++++++++++++++ workflows/readsimulator.nf | 104 ++++++++--------- 20 files changed, 1002 insertions(+), 70 deletions(-) create mode 100644 modules/local/capsim.nf create mode 100644 modules/local/crabs_dbimport.nf create mode 100644 modules/local/crabs_insilicopcr.nf create mode 100644 modules/local/pbsim.nf create mode 100644 modules/local/simlord.nf create mode 100644 modules/nf-core/art/illumina/art-illumina.diff create mode 100644 modules/nf-core/art/illumina/main.nf create mode 100644 modules/nf-core/art/illumina/meta.yml create mode 100644 modules/nf-core/bowtie2/align/main.nf create mode 100644 modules/nf-core/bowtie2/align/meta.yml create mode 100644 modules/nf-core/bowtie2/build/main.nf create mode 100644 modules/nf-core/bowtie2/build/meta.yml create mode 100644 modules/nf-core/samtools/index/main.nf create mode 100644 modules/nf-core/samtools/index/meta.yml create mode 100644 subworkflows/local/amplicon.nf create mode 100644 subworkflows/local/target_capture.nf diff --git a/conf/modules.config b/conf/modules.config index 8aaac49..556d29b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,6 +18,24 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] + withName: CAPSIM_ILLUMINA { + ext.args = "--fmedian 500 --num 500000 --illen 150" + ext.args2 = "--miseq" + publishDir = [ + path: { "${params.outdir}/capsim_illumina" }, + mode: params.publish_dir_mode + ] + } + + withName: CAPSIM_PACBIO { + ext.args = "--fmedian 500 --num 500000" + ext.args2 = "--pacbio" + publishDir = [ + path: { "${params.outdir}/capsim_pacbio" }, + mode: params.publish_dir_mode + ] + } + withName: CRABS_DBIMPORT { ext.args = "--seq_header accession --delim ' '" publishDir = [ diff --git a/modules/local/capsim.nf b/modules/local/capsim.nf new file mode 100644 index 0000000..90afbc8 --- /dev/null +++ b/modules/local/capsim.nf @@ -0,0 +1,42 @@ +process CAPSIM { + + tag "$meta.id" + label 'process_single' + + // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. + //conda "bioconda::crabs=0.1.1-0" + //container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + // 'https://depot.galaxyproject.org/singularity/crabs:0.1.1--pyhb7b1952_0': + // 'docker.io/vmurigneux/japsa' }" + container "docker.io/vmurigneux/japsa" + + input: + tuple val(meta), path(fasta), path(probes), path(index) + + output: + tuple val(meta), path("*.fastq.gz*"), emit: fastq + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def seed = task.ext.seed ?: "${meta.seed}" + def VERSION = '0.9-01a' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + jsa.sim.capsim \\ + --reference ${fasta} \\ + --probe ${probes} \\ + --ID ${prefix} \\ + --seed ${seed} \\ + ${args2} ${prefix} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + capsim: $VERSION + END_VERSIONS + """ +} diff --git a/modules/local/crabs_dbimport.nf b/modules/local/crabs_dbimport.nf new file mode 100644 index 0000000..12992b5 --- /dev/null +++ b/modules/local/crabs_dbimport.nf @@ -0,0 +1,37 @@ +process CRABS_DBIMPORT { + + tag "$meta.id" + label 'process_single' + + // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. + conda "bioconda::crabs=0.1.1-0" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/crabs:0.1.1--pyhb7b1952_0': + 'biocontainers/crabs:0.1.1--pyhb7b1952_0' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.fa"), emit: fasta + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def VERSION = '0.1.1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + crabs db_import \\ + --input $fasta \\ + --output ${prefix}.crabsdb.fa \\ + $args + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + crabs: $VERSION + END_VERSIONS + """ +} diff --git a/modules/local/crabs_insilicopcr.nf b/modules/local/crabs_insilicopcr.nf new file mode 100644 index 0000000..62b33f6 --- /dev/null +++ b/modules/local/crabs_insilicopcr.nf @@ -0,0 +1,37 @@ +process CRABS_INSILICOPCR { + + tag "$meta.id" + label 'process_single' + + // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. + conda "bioconda::crabs=0.1.1-0" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/crabs:0.1.1--pyhb7b1952_0': + 'biocontainers/crabs:0.1.1--pyhb7b1952_0' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.fa"), emit: fasta + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def VERSION = '0.1.1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + crabs insilico_pcr \\ + --input $fasta \\ + --output ${prefix}.crabs.fa \\ + $args + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + crabs: $VERSION + END_VERSIONS + """ +} diff --git a/modules/local/pbsim.nf b/modules/local/pbsim.nf new file mode 100644 index 0000000..17d378a --- /dev/null +++ b/modules/local/pbsim.nf @@ -0,0 +1,41 @@ +process PBSIM { + + tag "$meta.id" + label 'process_single' + + // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. + conda "bioconda::pbsim=1.0.3" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/pbsim:1.0.3--h4ac6f70_7': + 'biocontainers/pbsim:1.0.3--h4ac6f70_7' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.fastq.gz"), emit: fastq + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def seed = task.ext.seed ?: "${meta.seed}" + def VERSION = '1.0.3' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + pbsim \\ + --prefix $prefix \\ + --seed $seed \\ + $args \\ + $fasta + + gzip ${prefix}.fastq + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + pbsim: $VERSION + END_VERSIONS + """ +} diff --git a/modules/local/simlord.nf b/modules/local/simlord.nf new file mode 100644 index 0000000..71c291f --- /dev/null +++ b/modules/local/simlord.nf @@ -0,0 +1,38 @@ +process SIMLORD { + + tag "$meta.id" + label 'process_single' + + conda "bioconda::simlord=1.0.4" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/simlord:1.0.4--py310h4b81fae_3': + 'biocontainers/simlord:1.0.4--py310h4b81fae_3' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.fastq.gz"), emit: fastq + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def seed = task.ext.seed ?: "${meta.seed}" + """ + simlord \\ + --read-reference $fasta \\ + $args \\ + $prefix + + gzip ${prefix}.fastq + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + pbsim: \$(simlord --version) + END_VERSIONS + """ +} diff --git a/modules/nf-core/art/illumina/art-illumina.diff b/modules/nf-core/art/illumina/art-illumina.diff new file mode 100644 index 0000000..0f8e263 --- /dev/null +++ b/modules/nf-core/art/illumina/art-illumina.diff @@ -0,0 +1,37 @@ +Changes in module 'nf-core/art/illumina' +--- modules/nf-core/art/illumina/main.nf ++++ modules/nf-core/art/illumina/main.nf +@@ -12,7 +12,6 @@ + input: + tuple val(meta), path(fasta) + val(sequencing_system) +- val(fold_coverage) + val(read_length) + + output: +@@ -28,13 +27,14 @@ + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" ++ def seed = task.ext.prefix ?: "${meta.seed}" + def VERSION = '2016.06.05' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + art_illumina \\ + -ss $sequencing_system \\ + -i $fasta \\ + -l $read_length \\ +- -f $fold_coverage \\ ++ -rs $seed \\ + -o $prefix \\ + $args + + +--- modules/nf-core/art/illumina/meta.yml ++++ modules/nf-core/art/illumina/meta.yml +@@ -56,4 +56,4 @@ + pattern: "*.sam" + + authors: +- - "@MarieLataretu" ++ - "@MarieLataretu" +************************************************************ diff --git a/modules/nf-core/art/illumina/main.nf b/modules/nf-core/art/illumina/main.nf new file mode 100644 index 0000000..0d7978b --- /dev/null +++ b/modules/nf-core/art/illumina/main.nf @@ -0,0 +1,71 @@ +process ART_ILLUMINA { + + tag "$meta.id" + label 'process_single' + + // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. + conda "bioconda::art=2016.06.05" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/art:2016.06.05--h589041f_9': + 'biocontainers/art:2016.06.05--h589041f_9' }" + + input: + tuple val(meta), path(fasta) + val(sequencing_system) + val(read_length) + + output: + tuple val(meta), path("*.fq.gz"), emit: fastq + tuple val(meta), path("*.aln"), optional:true , emit: aln + tuple val(meta), path("*.sam"), optional:true , emit: sam + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def seed = task.ext.prefix ?: "${meta.seed}" + def VERSION = '2016.06.05' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + art_illumina \\ + -ss $sequencing_system \\ + -i $fasta \\ + -l $read_length \\ + -rs $seed \\ + -o $prefix \\ + $args + + gzip \\ + --no-name \\ + $args2 \\ + $prefix*.fq + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + art: $VERSION + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def VERSION = '2016.06.05' + """ + touch ${prefix}.fq.gz + touch ${prefix}1.fq.gz + touch ${prefix}2.fq.gz + touch ${prefix}.aln + touch ${prefix}1.aln + touch ${prefix}2.aln + touch ${prefix}.sam + touch ${prefix}1.sam + touch ${prefix}2.sam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + art: $VERSION + END_VERSIONS + """ +} diff --git a/modules/nf-core/art/illumina/meta.yml b/modules/nf-core/art/illumina/meta.yml new file mode 100644 index 0000000..dad0bcd --- /dev/null +++ b/modules/nf-core/art/illumina/meta.yml @@ -0,0 +1,59 @@ +name: "art_illumina" +description: Simulation tool to generate synthetic Illumina next-generation sequencing reads +keywords: + - fastq + - fasta + - illumina + - simulate +tools: + - "art": + description: "ART is a set of simulation tools to generate synthetic next-generation sequencing reads. ART simulates sequencing reads by mimicking real sequencing process with empirical error models or quality profiles summarized from large recalibrated sequencing data. ART can also simulate reads using user own read error model or quality profiles. " + homepage: "https://www.niehs.nih.gov/research/resources/software/biostatistics/art/index.cfm" + doi: "10.1093/bioinformatics/btr708" + licence: "GPL version 3 license" + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test' ] + - fasta: + type: file + description: FASTA file of input DNA/RNA reference + pattern: "*.{fasta,fa}" + - sequencing_system: + type: string + description: The name of Illumina sequencing system of the built-in profile used for simulation + - fold_coverage: + type: integer + description: The fold of read coverage to be simulated or number of reads/read pairs generated for each amplicon + - read_length: + type: integer + description: The length of reads to be simulated + +output: + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - fastq: + type: file + description: Simulated reads + pattern: "*.fq.gz" + - aln: + type: file + description: OPTIONAL Alignment file of the simulated reads. Enabled by default, to disable, use -na/--noALN. + pattern: "*.aln" + - sam: + type: file + description: OPTIONAL Alignment file in SAM format of the simulated reads. Enabled with -sam/--samout. + pattern: "*.sam" + +authors: + - "@MarieLataretu" \ No newline at end of file diff --git a/modules/nf-core/bowtie2/align/main.nf b/modules/nf-core/bowtie2/align/main.nf new file mode 100644 index 0000000..a77114d --- /dev/null +++ b/modules/nf-core/bowtie2/align/main.nf @@ -0,0 +1,94 @@ +process BOWTIE2_ALIGN { + tag "$meta.id" + label "process_high" + + conda "bioconda::bowtie2=2.4.4 bioconda::samtools=1.16.1 conda-forge::pigz=2.6" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' : + 'biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' }" + + input: + tuple val(meta) , path(reads) + tuple val(meta2), path(index) + val save_unaligned + val sort_bam + + output: + tuple val(meta), path("*.{bam,sam}"), emit: aligned + tuple val(meta), path("*.log") , emit: log + tuple val(meta), path("*fastq.gz") , emit: fastq, optional:true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: "" + def args2 = task.ext.args2 ?: "" + def prefix = task.ext.prefix ?: "${meta.id}" + + def unaligned = "" + def reads_args = "" + if (meta.single_end) { + unaligned = save_unaligned ? "--un-gz ${prefix}.unmapped.fastq.gz" : "" + reads_args = "-U ${reads}" + } else { + unaligned = save_unaligned ? "--un-conc-gz ${prefix}.unmapped.fastq.gz" : "" + reads_args = "-1 ${reads[0]} -2 ${reads[1]}" + } + + def samtools_command = sort_bam ? 'sort' : 'view' + def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/ + def extension = (args2 ==~ extension_pattern) ? (args2 =~ extension_pattern)[0][2].toLowerCase() : "bam" + + """ + INDEX=`find -L ./ -name "*.rev.1.bt2" | sed "s/\\.rev.1.bt2\$//"` + [ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed "s/\\.rev.1.bt2l\$//"` + [ -z "\$INDEX" ] && echo "Bowtie2 index files not found" 1>&2 && exit 1 + + bowtie2 \\ + -x \$INDEX \\ + $reads_args \\ + --threads $task.cpus \\ + $unaligned \\ + $args \\ + 2> ${prefix}.bowtie2.log \\ + | samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.${extension} - + + if [ -f ${prefix}.unmapped.fastq.1.gz ]; then + mv ${prefix}.unmapped.fastq.1.gz ${prefix}.unmapped_1.fastq.gz + fi + + if [ -f ${prefix}.unmapped.fastq.2.gz ]; then + mv ${prefix}.unmapped.fastq.2.gz ${prefix}.unmapped_2.fastq.gz + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bowtie2: \$(echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//') + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) + END_VERSIONS + """ + + stub: + def args2 = task.ext.args2 ?: "" + def prefix = task.ext.prefix ?: "${meta.id}" + def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/ + def extension = (args2 ==~ extension_pattern) ? (args2 =~ extension_pattern)[0][2].toLowerCase() : "bam" + + """ + touch ${prefix}.${extension} + touch ${prefix}.bowtie2.log + touch ${prefix}.unmapped_1.fastq.gz + touch ${prefix}.unmapped_2.fastq.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bowtie2: \$(echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//') + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) + END_VERSIONS + """ + +} diff --git a/modules/nf-core/bowtie2/align/meta.yml b/modules/nf-core/bowtie2/align/meta.yml new file mode 100644 index 0000000..60d04c1 --- /dev/null +++ b/modules/nf-core/bowtie2/align/meta.yml @@ -0,0 +1,67 @@ +name: bowtie2_align +description: Align reads to a reference genome using bowtie2 +keywords: + - align + - map + - fasta + - fastq + - genome + - reference +tools: + - bowtie2: + description: | + Bowtie 2 is an ultrafast and memory-efficient tool for aligning + sequencing reads to long reference sequences. + homepage: http://bowtie-bio.sourceforge.net/bowtie2/index.shtml + documentation: http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml + doi: 10.1038/nmeth.1923 + licence: ["GPL-3.0-or-later"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test', single_end:false ] + - index: + type: file + description: Bowtie2 genome index files + pattern: "*.ebwt" + - save_unaligned: + type: boolean + description: | + Save reads that do not map to the reference (true) or discard them (false) + (default: false) + - sort_bam: + type: boolean + description: use samtools sort (true) or samtools view (false) + pattern: "true or false" +output: + - aligned: + type: file + description: Output BAM/SAM file containing read alignments + pattern: "*.{bam,sam}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - fastq: + type: file + description: Unaligned FastQ files + pattern: "*.fastq.gz" + - log: + type: file + description: Aligment log + pattern: "*.log" +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/bowtie2/build/main.nf b/modules/nf-core/bowtie2/build/main.nf new file mode 100644 index 0000000..069d9c1 --- /dev/null +++ b/modules/nf-core/bowtie2/build/main.nf @@ -0,0 +1,42 @@ +process BOWTIE2_BUILD { + tag "$fasta" + label 'process_high' + + conda "bioconda::bowtie2=2.4.4" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bowtie2:2.4.4--py39hbb4e92a_0' : + 'biocontainers/bowtie2:2.4.4--py39hbb4e92a_0' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path('bowtie2') , emit: index + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + """ + mkdir bowtie2 + bowtie2-build $args --threads $task.cpus $fasta bowtie2/${fasta.baseName} + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bowtie2: \$(echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//') + END_VERSIONS + """ + + stub: + """ + mkdir bowtie2 + touch bowtie2/${fasta.baseName}.{1..4}.bt2 + touch bowtie2/${fasta.baseName}.rev.{1,2}.bt2 + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bowtie2: \$(echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bowtie2/build/meta.yml b/modules/nf-core/bowtie2/build/meta.yml new file mode 100644 index 0000000..0240224 --- /dev/null +++ b/modules/nf-core/bowtie2/build/meta.yml @@ -0,0 +1,43 @@ +name: bowtie2_build +description: Builds bowtie index for reference genome +keywords: + - build + - index + - fasta + - genome + - reference +tools: + - bowtie2: + description: | + Bowtie 2 is an ultrafast and memory-efficient tool for aligning + sequencing reads to long reference sequences. + homepage: http://bowtie-bio.sourceforge.net/bowtie2/index.shtml + documentation: http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml + doi: 10.1038/nmeth.1923 + licence: ["GPL-3.0-or-later"] +input: + - meta: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test', single_end:false ] + - fasta: + type: file + description: Input genome fasta file +output: + - meta: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test', single_end:false ] + - index: + type: file + description: Bowtie2 genome index files + pattern: "*.bt2" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf new file mode 100644 index 0000000..0b20aa4 --- /dev/null +++ b/modules/nf-core/samtools/index/main.nf @@ -0,0 +1,48 @@ +process SAMTOOLS_INDEX { + tag "$meta.id" + label 'process_low' + + conda "bioconda::samtools=1.17" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : + 'biocontainers/samtools:1.17--h00cdaf9_0' }" + + input: + tuple val(meta), path(input) + + output: + tuple val(meta), path("*.bai") , optional:true, emit: bai + tuple val(meta), path("*.csi") , optional:true, emit: csi + tuple val(meta), path("*.crai"), optional:true, emit: crai + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + """ + samtools \\ + index \\ + -@ ${task.cpus-1} \\ + $args \\ + $input + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + """ + touch ${input}.bai + touch ${input}.crai + touch ${input}.csi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml new file mode 100644 index 0000000..8bd2fa6 --- /dev/null +++ b/modules/nf-core/samtools/index/meta.yml @@ -0,0 +1,53 @@ +name: samtools_index +description: Index SAM/BAM/CRAM file +keywords: + - index + - bam + - sam + - cram +tools: + - samtools: + description: | + SAMtools is a set of utilities for interacting with and post-processing + short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. + These files are generated as output by short read aligners like BWA. + homepage: http://www.htslib.org/ + documentation: http://www.htslib.org/doc/samtools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bai: + type: file + description: BAM/CRAM/SAM index file + pattern: "*.{bai,crai,sai}" + - crai: + type: file + description: BAM/CRAM/SAM index file + pattern: "*.{bai,crai,sai}" + - csi: + type: file + description: CSI index file + pattern: "*.{csi}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@ewels" + - "@maxulysse" diff --git a/nextflow.config b/nextflow.config index 1834db1..b8f36e8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -19,14 +19,17 @@ params { igenomes_ignore = false fasta = null - //seeds = null - //prefix = null - fw_primer = 'GTCGGTAAAACTCGTGCCAGC' - rv_primer = 'CATAGTGGGGTATCTAATCCCAGTTTG' + fw_primer = 'GTCGGTAAAACTCGTGCCAGC' + rv_primer = 'CATAGTGGGGTATCTAATCCCAGTTTG' // Simulation options - //illumina_amplicon = null - //illumina_uce = null + amplicon = null + target_capture = null + + // Provider options + illumina = null + pacbio = null + nanopore = null // MultiQC options multiqc_config = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 39502b2..573f894 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -49,14 +49,37 @@ "fa_icon": "fas fa-terminal", "description": "Choose the data types that should be simulated by the pipeline.", "properties": { - "illumina_amplicon": { + "amplicon": { "type": "boolean", - "description": "Option to simulate Illumina amplicon data.", + "description": "Option to simulate amplicon data.", "fa_icon": "fas fa-question-circle" }, - "illumina_uce": { + "target_capturee": { "type": "boolean", - "description": "Option to simulate Illumina UCE data.", + "description": "Option to simulate UCE target capture data.", + "fa_icon": "fas fa-question-circle" + } + } + }, + "provider_options": { + "title": "Provider options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Choose the sequencing provider of the simulated reads.", + "properties": { + "illumina": { + "type": "boolean", + "description": "Option to simulate illumina data.", + "fa_icon": "fas fa-question-circle" + }, + "pacbio": { + "type": "boolean", + "description": "Option to simulate pacbio data.", + "fa_icon": "fas fa-question-circle" + }, + "nanopore": { + "type": "boolean", + "description": "Option to simulate nanopore data.", "fa_icon": "fas fa-question-circle" } } diff --git a/subworkflows/local/amplicon.nf b/subworkflows/local/amplicon.nf new file mode 100644 index 0000000..72a324a --- /dev/null +++ b/subworkflows/local/amplicon.nf @@ -0,0 +1,90 @@ +// +// Simulate amplicon reads +// + +include { CRABS_DBIMPORT } from '../../modules/local/crabs_dbimport' +include { CRABS_INSILICOPCR } from '../../modules/local/crabs_insilicopcr' +include { ART_ILLUMINA } from '../../modules/nf-core/art/illumina/main' +include { PBSIM } from '../../modules/local/pbsim' +include { SIMLORD } from '../../modules/local/simlord' + +workflow AMPLICON { + ch_versions = Channel.empty() + + take: + ch_fasta // file: /path/to/reference.fasta + ch_input // channel: [ meta ] + + main: + // Add a meta map to fasta channel for compatibility with modules + ch_meta_fasta = ch_fasta + .map { + fasta -> + def meta = [:] + meta.id = "illumina_amplicon" + return [ meta, fasta ] + } + + // + // MODULE: Run Crabs db_import + // + CRABS_DBIMPORT ( + ch_meta_fasta + ) + ch_versions = ch_versions.mix(CRABS_DBIMPORT.out.versions.first()) + + // + // MODULE: Run Crabs insilico_pcr + // + CRABS_INSILICOPCR ( + CRABS_DBIMPORT.out.fasta + ) + ch_versions = ch_versions.mix(CRABS_INSILICOPCR.out.versions.first()) + + // Now that we have processed our fasta file, + // we need to map it to our sample data + ch_art_input = CRABS_INSILICOPCR.out.fasta + .combine ( ch_input ) + .map { + it = [ it[2], it[1] ] + } + //.map { + // meta, fasta -> + // meta.id = "illumina_amplicon_" + meta.id + // return [ meta, fasta ] + //} + + // + // MODULE: Simulate Illumina reads + // + ch_illumina_reads = Channel.empty() + if ( params.illumina ) { + ART_ILLUMINA ( + ch_art_input, + "HS25", + 130 + ) + ch_versions = ch_versions.mix(ART_ILLUMINA.out.versions.first()) + ch_illumina_reads = ART_ILLUMINA.out.fastq + } + + // Pacbio simulators, + // PBSIM is expecting a file that's not in the container, maybe it can be pulled? + // SIMLORD works, but doesn't have a seed parameter, maybe it's using the internal clock for the seed? + //PBSIM ( + // ch_art_input + //) + //SIMLORD ( + // ch_art_input + //) + + // Nanopore simulator + //NANOSIM () + + // BAD READ simulator + //BADREAD () + + emit: + illumina_reads = ch_illumina_reads // channel: [ meta, fastq ] + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/subworkflows/local/target_capture.nf b/subworkflows/local/target_capture.nf new file mode 100644 index 0000000..d34aefd --- /dev/null +++ b/subworkflows/local/target_capture.nf @@ -0,0 +1,105 @@ +// +// Simulate UCE target capture reads +// + +include { BOWTIE2_BUILD } from '../../modules/nf-core/bowtie2/build/main' +include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' +include { CAPSIM as CAPSIM_ILLUMINA } from '../../modules/local/capsim' +include { CAPSIM as CAPSIM_PACBIO } from '../../modules/local/capsim' + +workflow TARGET_CAPTURE { + take: + ch_fasta // file: /path/to/reference.fasta + ch_input // channel: [ meta ] + ch_probes // file: /path/to/probes.fasta + + main: + ch_versions = Channel.empty() + + // Add a meta map to fasta channel for compatibility with modules + ch_meta_fasta = ch_fasta + .map { + fasta -> + def meta = [:] + meta.id = "illumina_uce" + return [ meta, fasta ] + } + + // + // MODULE: Create Bowtie index + // + BOWTIE2_BUILD ( + ch_meta_fasta + ) + ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions.first()) + + ch_probes = ch_probes + .map { + meta, fasta -> + meta.single_end = true + return [ meta, fasta ] + } + // + // MODULE: Align probes to genome + // + BOWTIE2_ALIGN ( + ch_probes, + BOWTIE2_BUILD.out.index, + false, + false + ) + ch_versions = ch_versions.mix(BOWTIE2_ALIGN.out.versions.first()) + + // + // MODULES: Get SAM index + // + SAMTOOLS_INDEX ( + BOWTIE2_ALIGN.out.aligned + ) + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) + + // Now that we have our fasta file + BAM file + index, + // we need to map them to our sample data + ch_capsim_input = ch_meta_fasta.map { it = it[1] } + .combine ( BOWTIE2_ALIGN.out.aligned.map { it = it[1] } ) + .combine ( SAMTOOLS_INDEX.out.bai.map { it = it[1] } ) + .combine ( ch_input ) + .map { + it = [ it[3], it[0], it[1], it[2] ] + } + //.map { + // meta, fasta, bam, index -> + // meta.id = "illumina_taget_capture_" + meta.id + // return [ meta, fasta, bam, index ] + //} + + // + // MODULE: Simulate target capture illumina reads + // + ch_illumina_reads = Channel.empty() + if ( params.illumina ) { + CAPSIM_ILLUMINA ( + ch_capsim_input + ) + ch_versions = ch_versions.mix(CAPSIM_ILLUMINA.out.versions.first()) + ch_illumina_reads = CAPSIM_ILLUMINA.out.fastq + } + + // + // MODULE: Simulate target capture pacbio reads + // + ch_pacbio_reads = Channel.empty() + if ( params.pacbio ) { + CAPSIM_PACBIO ( + ch_capsim_input + ) + ch_versions = ch_versions.mix(CAPSIM_PACBIO.out.versions.first()) + ch_pacbio_reads = CAPSIM_PACBIO.out.fastq + } + + emit: + illumina_reads = ch_illumina_reads // channel: [ meta, fastq ] + pacbio_reads = ch_pacbio_reads // channel: [ meta, fastq ] + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 540c29f..63cf4aa 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -35,8 +35,8 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { ILLUMINA_AMPLICON } from '../subworkflows/local/illumina_amplicon' -include { ILLUMINA_UCE } from '../subworkflows/local/illumina_uce' +include { AMPLICON } from '../subworkflows/local/amplicon' +include { TARGET_CAPTURE } from '../subworkflows/local/target_capture' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -64,56 +64,40 @@ workflow READSIMULATOR { ch_versions = Channel.empty() ch_fasta = Channel.fromPath(params.fasta) - //ch_fasta = ch_fasta.map { - // fasta -> - // def meta = [:] - // meta.id = params.prefix - // [ meta, fasta ] - //} - //ls_seeds = params.seeds?.tokenize(',') - //ch_seed = Channel.fromList(ls_seeds) - - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files - // - //INPUT_CHECK ( - // file(params.input) - //) - //ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - ch_input = Channel.fromSamplesheet("input") - // .map { meta -> - // def fasta = file(params.fasta) - // return [ meta[0], fasta ] - // } + ch_fastq_input = Channel.empty() // - // SUBWORKFLOW: Simulate illumina amplicon reads + // SUBWORKFLOW: Simulate amplicon reads // - if ( params.illumina_amplicon ) { - ILLUMINA_AMPLICON ( + if ( params.amplicon ) { + AMPLICON ( ch_fasta, ch_input ) - ch_versions = ch_versions.mix(ILLUMINA_AMPLICON.out.versions.first()) + ch_versions = ch_versions.mix(AMPLICON.out.versions.first()) + ch_fastq_input = ch_fastq_input.mix(AMPLICON.out.illumina_reads) } // - // SUBWORKFLOW: Simulate illumina UCE reads + // SUBWORKFLOW: Simulate UCE taget capture reads // - if ( params.illumina_uce ) { + if ( params.target_capture ) { ch_probes = Channel.fromPath(params.probes) - ch_probes = ch_probes.map { - fasta -> - def meta = [:] - meta.id = "probes" - [ meta, fasta ] - } - ILLUMINA_UCE ( + ch_probes = ch_probes.map { + fasta -> + def meta = [:] + meta.id = "probes" + [ meta, fasta ] + } + TARGET_CAPTURE ( ch_fasta, + ch_input, ch_probes ) - ch_versions = ch_versions.mix(ILLUMINA_UCE.out.versions.first()) + ch_versions = ch_versions.mix(TARGET_CAPTURE.out.versions.first()) + ch_fastq_input = ch_fastq_input.mix(TARGET_CAPTURE.out.illumina_reads) + ch_fastq_input = ch_fastq_input.mix(TARGET_CAPTURE.out.pacbio_reads) } // MODULE: Create sample sheet @@ -122,10 +106,10 @@ workflow READSIMULATOR { // // MODULE: Run FastQC // - //FASTQC ( - // ILLUMINA_AMPLICON.out.reads - //) - //ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + FASTQC ( + ch_fastq_input + ) + ch_versions = ch_versions.mix(FASTQC.out.versions.first()) CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') @@ -134,25 +118,25 @@ workflow READSIMULATOR { // // MODULE: MultiQC // - //workflow_summary = WorkflowReadsimulator.paramsSummaryMultiqc(workflow, summary_params) - //ch_workflow_summary = Channel.value(workflow_summary) - - //methods_description = WorkflowReadsimulator.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - //ch_methods_description = Channel.value(methods_description) - - //ch_multiqc_files = Channel.empty() - //ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - //ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - //ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - //ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) - - //MULTIQC ( - // ch_multiqc_files.collect(), - // ch_multiqc_config.toList(), - // ch_multiqc_custom_config.toList(), - // ch_multiqc_logo.toList() - //) - //multiqc_report = MULTIQC.out.report.toList() + workflow_summary = WorkflowReadsimulator.paramsSummaryMultiqc(workflow, summary_params) + ch_workflow_summary = Channel.value(workflow_summary) + + methods_description = WorkflowReadsimulator.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) + ch_methods_description = Channel.value(methods_description) + + ch_multiqc_files = Channel.empty() + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + + MULTIQC ( + ch_multiqc_files.collect(), + ch_multiqc_config.toList(), + ch_multiqc_custom_config.toList(), + ch_multiqc_logo.toList() + ) + multiqc_report = MULTIQC.out.report.toList() } /* From 8af7d0539dc79070be4c6a3f14b7d43e03acf9bb Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 28 Sep 2023 02:34:37 +0000 Subject: [PATCH 03/89] testing other pacbio simulators --- conf/modules.config | 4 +- modules/local/badread.nf | 37 +++++++++++++++ modules/local/nanosim.nf | 39 ++++++++++++++++ modules/local/pbsim.nf | 5 ++- nextflow.config | 1 + nextflow_schema.json | 9 +++- .../{amplicon.nf => amplicon_workflow.nf} | 45 ++++++++++++------- ..._capture.nf => target_capture_workflow.nf} | 2 +- workflows/readsimulator.nf | 18 ++++---- 9 files changed, 130 insertions(+), 30 deletions(-) create mode 100644 modules/local/badread.nf create mode 100644 modules/local/nanosim.nf rename subworkflows/local/{amplicon.nf => amplicon_workflow.nf} (69%) rename subworkflows/local/{target_capture.nf => target_capture_workflow.nf} (98%) diff --git a/conf/modules.config b/conf/modules.config index 556d29b..9837191 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -77,7 +77,9 @@ process { } withName: PBSIM { - ext.args = "--model_qc" + ext.args = "--data-type CLR --model_qc " + //ext.args = "--data-type CLR --model_qc /usr/share/pbsim/models/model_qc_clr" + //"--data-type CCS --model_qc /usr/share/pbsim/models/model_qc_ccs" publishDir = [ path: { "${params.outdir}/pbsim" }, mode: params.publish_dir_mode diff --git a/modules/local/badread.nf b/modules/local/badread.nf new file mode 100644 index 0000000..7e8f9bd --- /dev/null +++ b/modules/local/badread.nf @@ -0,0 +1,37 @@ +process BADREAD { + + tag "$meta.id" + label 'process_single' + + conda "bioconda::badread=0.4.0" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/badread:0.4.0--pyhdfd78af_1': + 'biocontainers/badread:0.4.0--pyhdfd78af_1' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.fastq.gz*"), emit: fastq + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def seed = task.ext.seed ?: "${meta.seed}" + """ + badread simulate \\ + --reference ${fasta} \\ + --seed ${seed} \\ + --quantity 50x + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + badread: \$(badread simulate --version) + END_VERSIONS + """ +} diff --git a/modules/local/nanosim.nf b/modules/local/nanosim.nf new file mode 100644 index 0000000..c0344fc --- /dev/null +++ b/modules/local/nanosim.nf @@ -0,0 +1,39 @@ +process NANOSIM { + + tag "$meta.id" + label 'process_single' + + conda "bioconda::nanosim=3.1.0" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/nanosim:3.1.0--hdfd78af_0': + 'biocontainers/nanosim:3.1.0--hdfd78af_0' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.fastq.gz*"), emit: fastq + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def seed = task.ext.seed ?: "${meta.seed}" + """ + read_analysis.py metagenome \\ + --read ${fasta} \\ + --probe ${probes} \\ + --ID ${prefix} \\ + --seed ${seed} \\ + ${args2} ${prefix} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + nanosim: \$(read_analysis.py --version) + END_VERSIONS + """ +} diff --git a/modules/local/pbsim.nf b/modules/local/pbsim.nf index 17d378a..c8a941a 100644 --- a/modules/local/pbsim.nf +++ b/modules/local/pbsim.nf @@ -11,6 +11,7 @@ process PBSIM { input: tuple val(meta), path(fasta) + path(model) output: tuple val(meta), path("*.fastq.gz"), emit: fastq @@ -28,10 +29,10 @@ process PBSIM { pbsim \\ --prefix $prefix \\ --seed $seed \\ - $args \\ + $args $model \\ $fasta - gzip ${prefix}.fastq + gzip ${prefix}*.fastq cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/nextflow.config b/nextflow.config index b8f36e8..0ac2c82 100644 --- a/nextflow.config +++ b/nextflow.config @@ -17,6 +17,7 @@ params { //igenomes_base = 's3://ngi-igenomes/igenomes' igenomes_base = null igenomes_ignore = false + pbsim_model = 'https://raw.githubusercontent.com/a4000/test_data/main/models/model_qc_clr' fasta = null fw_primer = 'GTCGGTAAAACTCGTGCCAGC' diff --git a/nextflow_schema.json b/nextflow_schema.json index 573f894..15f6278 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -40,6 +40,13 @@ "type": "string", "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", "fa_icon": "fas fa-file-signature" + }, + "pbsim_model": { + "type": "string", + "format": "file-path", + "exists": true, + "description": "Path to pbsim model.", + "fa_icon": "fas fa-file-signature" } } }, @@ -54,7 +61,7 @@ "description": "Option to simulate amplicon data.", "fa_icon": "fas fa-question-circle" }, - "target_capturee": { + "target_capture": { "type": "boolean", "description": "Option to simulate UCE target capture data.", "fa_icon": "fas fa-question-circle" diff --git a/subworkflows/local/amplicon.nf b/subworkflows/local/amplicon_workflow.nf similarity index 69% rename from subworkflows/local/amplicon.nf rename to subworkflows/local/amplicon_workflow.nf index 72a324a..407b137 100644 --- a/subworkflows/local/amplicon.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -7,8 +7,10 @@ include { CRABS_INSILICOPCR } from '../../modules/local/crabs_insilicopcr' include { ART_ILLUMINA } from '../../modules/nf-core/art/illumina/main' include { PBSIM } from '../../modules/local/pbsim' include { SIMLORD } from '../../modules/local/simlord' +include { BADREAD } from '../../modules/local/badread' +include { NANOSIM } from '../../modules/local/nanosim' -workflow AMPLICON { +workflow AMPLICON_WORKFLOW { ch_versions = Channel.empty() take: @@ -43,7 +45,7 @@ workflow AMPLICON { // Now that we have processed our fasta file, // we need to map it to our sample data - ch_art_input = CRABS_INSILICOPCR.out.fasta + ch_meta_fasta = CRABS_INSILICOPCR.out.fasta .combine ( ch_input ) .map { it = [ it[2], it[1] ] @@ -60,7 +62,7 @@ workflow AMPLICON { ch_illumina_reads = Channel.empty() if ( params.illumina ) { ART_ILLUMINA ( - ch_art_input, + ch_meta_fasta, "HS25", 130 ) @@ -68,23 +70,34 @@ workflow AMPLICON { ch_illumina_reads = ART_ILLUMINA.out.fastq } - // Pacbio simulators, - // PBSIM is expecting a file that's not in the container, maybe it can be pulled? - // SIMLORD works, but doesn't have a seed parameter, maybe it's using the internal clock for the seed? - //PBSIM ( - // ch_art_input - //) - //SIMLORD ( - // ch_art_input - //) + // + // MODULE: Simulate pacbio reads + // + ch_pacbio_reads = Channel.empty() + if ( params.pacbio ) { + ch_model = Channel.fromPath(params.pbsim_model) + PBSIM ( + ch_meta_fasta, + ch_model.first() + ) + ch_versions = ch_versions.mix(PBSIM.out.versions.first()) + ch_pacbio_reads = PBSIM.out.fastq + //SIMLORD ( + // ch_meta_fasta + //) + //ch_pacbio_reads = SIMLORD.out.fastq + } - // Nanopore simulator - //NANOSIM () + //BADREAD ( + // ch_meta_fasta + //) - // BAD READ simulator - //BADREAD () + //NANOSIM ( + // ch_meta_fasta + //) emit: illumina_reads = ch_illumina_reads // channel: [ meta, fastq ] + pacbio_reads = ch_pacbio_reads // channel: [ meta, fastq ] versions = ch_versions // channel: [ versions.yml ] } diff --git a/subworkflows/local/target_capture.nf b/subworkflows/local/target_capture_workflow.nf similarity index 98% rename from subworkflows/local/target_capture.nf rename to subworkflows/local/target_capture_workflow.nf index d34aefd..3c993cb 100644 --- a/subworkflows/local/target_capture.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -8,7 +8,7 @@ include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index include { CAPSIM as CAPSIM_ILLUMINA } from '../../modules/local/capsim' include { CAPSIM as CAPSIM_PACBIO } from '../../modules/local/capsim' -workflow TARGET_CAPTURE { +workflow TARGET_CAPTURE_WORKFLOW { take: ch_fasta // file: /path/to/reference.fasta ch_input // channel: [ meta ] diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 63cf4aa..4ddf8ec 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -35,8 +35,8 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { AMPLICON } from '../subworkflows/local/amplicon' -include { TARGET_CAPTURE } from '../subworkflows/local/target_capture' +include { AMPLICON_WORKFLOW } from '../subworkflows/local/amplicon_workflow' +include { TARGET_CAPTURE_WORKFLOW } from '../subworkflows/local/target_capture_workflow' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -71,12 +71,12 @@ workflow READSIMULATOR { // SUBWORKFLOW: Simulate amplicon reads // if ( params.amplicon ) { - AMPLICON ( + AMPLICON_WORKFLOW ( ch_fasta, ch_input ) - ch_versions = ch_versions.mix(AMPLICON.out.versions.first()) - ch_fastq_input = ch_fastq_input.mix(AMPLICON.out.illumina_reads) + ch_versions = ch_versions.mix(AMPLICON_WORKFLOW.out.versions.first()) + ch_fastq_input = ch_fastq_input.mix(AMPLICON_WORKFLOW.out.illumina_reads) } // @@ -90,14 +90,14 @@ workflow READSIMULATOR { meta.id = "probes" [ meta, fasta ] } - TARGET_CAPTURE ( + TARGET_CAPTURE_WORKFLOW ( ch_fasta, ch_input, ch_probes ) - ch_versions = ch_versions.mix(TARGET_CAPTURE.out.versions.first()) - ch_fastq_input = ch_fastq_input.mix(TARGET_CAPTURE.out.illumina_reads) - ch_fastq_input = ch_fastq_input.mix(TARGET_CAPTURE.out.pacbio_reads) + ch_versions = ch_versions.mix(TARGET_CAPTURE_WORKFLOW.out.versions.first()) + ch_fastq_input = ch_fastq_input.mix(TARGET_CAPTURE_WORKFLOW.out.illumina_reads) + ch_fastq_input = ch_fastq_input.mix(TARGET_CAPTURE_WORKFLOW.out.pacbio_reads) } // MODULE: Create sample sheet From 23241e84ebbb75527782dec1b3313f56044867c2 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Fri, 29 Sep 2023 04:55:07 +0000 Subject: [PATCH 04/89] added crabs_dbdownload module --- conf/modules.config | 34 +++--- lib/WorkflowReadsimulator.groovy | 1 - modules/local/badread.nf | 37 ------ modules/local/crabs_dbdownload.nf | 34 ++++++ modules/local/nanosim.nf | 39 ------- modules/local/pbsim.nf | 42 ------- modules/local/simlord.nf | 38 ------- nextflow.config | 23 ++-- nextflow_schema.json | 86 ++++++++------ subworkflows/local/amplicon_workflow.nf | 105 +++++++----------- subworkflows/local/target_capture_workflow.nf | 23 ++-- workflows/readsimulator.nf | 2 +- 12 files changed, 167 insertions(+), 297 deletions(-) delete mode 100644 modules/local/badread.nf create mode 100644 modules/local/crabs_dbdownload.nf delete mode 100644 modules/local/nanosim.nf delete mode 100644 modules/local/pbsim.nf delete mode 100644 modules/local/simlord.nf diff --git a/conf/modules.config b/conf/modules.config index 9837191..857cada 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -36,6 +36,22 @@ process { ] } + withName: CRABS_DBDOWNLOAD { + ext.args = + params.amplicon_ref_source == "ncbi" ? + "--source ncbi --database ${params.amplicon_ncbi_db} --query ${params.amplicon_ncbi_query} --species ${params.amplicon_ncbi_species} --email ${params.amplicon_ncbi_email} --batchsize ${params.amplicon_ncbi_batchsize} --keep_original no --output ncbi.fasta" : + params.amplicon_ref_source == "embl" ? + "--source embl --database ${params.amplicon_embl_db} --output embl.fasta --keep_original no" : + params.amplicon_ref_source == "bold" ? + "--source bold --database '${params.amplicon_bold_db}' --keep_original no --output bold.fasta" : + params.amplicon_ref_source == "mitofish" ? + "--source mitofish --keep_original no --output mitofish.fasta" : "" + publishDir = [ + path: { "${params.outdir}/crabs_dbdownload" }, + mode: params.publish_dir_mode + ] + } + withName: CRABS_DBIMPORT { ext.args = "--seq_header accession --delim ' '" publishDir = [ @@ -76,24 +92,6 @@ process { ] } - withName: PBSIM { - ext.args = "--data-type CLR --model_qc " - //ext.args = "--data-type CLR --model_qc /usr/share/pbsim/models/model_qc_clr" - //"--data-type CCS --model_qc /usr/share/pbsim/models/model_qc_ccs" - publishDir = [ - path: { "${params.outdir}/pbsim" }, - mode: params.publish_dir_mode - ] - } - - //withName: SAMPLESHEET_CHECK { - // publishDir = [ - // path: { "${params.outdir}/pipeline_info" }, - // mode: params.publish_dir_mode, - // saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - // ] - //} - withName: FASTQC { ext.args = '--quiet' } diff --git a/lib/WorkflowReadsimulator.groovy b/lib/WorkflowReadsimulator.groovy index c7cc20d..3c3a765 100755 --- a/lib/WorkflowReadsimulator.groovy +++ b/lib/WorkflowReadsimulator.groovy @@ -14,7 +14,6 @@ class WorkflowReadsimulator { genomeExistsError(params, log) - if (!params.fasta) { Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." } diff --git a/modules/local/badread.nf b/modules/local/badread.nf deleted file mode 100644 index 7e8f9bd..0000000 --- a/modules/local/badread.nf +++ /dev/null @@ -1,37 +0,0 @@ -process BADREAD { - - tag "$meta.id" - label 'process_single' - - conda "bioconda::badread=0.4.0" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/badread:0.4.0--pyhdfd78af_1': - 'biocontainers/badread:0.4.0--pyhdfd78af_1' }" - - input: - tuple val(meta), path(fasta) - - output: - tuple val(meta), path("*.fastq.gz*"), emit: fastq - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def seed = task.ext.seed ?: "${meta.seed}" - """ - badread simulate \\ - --reference ${fasta} \\ - --seed ${seed} \\ - --quantity 50x - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - badread: \$(badread simulate --version) - END_VERSIONS - """ -} diff --git a/modules/local/crabs_dbdownload.nf b/modules/local/crabs_dbdownload.nf new file mode 100644 index 0000000..9796e43 --- /dev/null +++ b/modules/local/crabs_dbdownload.nf @@ -0,0 +1,34 @@ +process CRABS_DBDOWNLOAD { + + tag "" + label 'process_single' + + // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. + conda "bioconda::crabs=0.1.1-0" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/crabs:0.1.1--pyhb7b1952_0': + 'swordfish/crabs:0.1.4' }" // this container works better than biocontainers/crabs:0.1.1--pyhb7b1952_0 + // no it doesn't, it just gives me a different error message + + input: + + output: + path("*.fasta") , emit: fasta + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def VERSION = '0.1.1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + crabs db_download \\ + $args + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + crabs: $VERSION + END_VERSIONS + """ +} diff --git a/modules/local/nanosim.nf b/modules/local/nanosim.nf deleted file mode 100644 index c0344fc..0000000 --- a/modules/local/nanosim.nf +++ /dev/null @@ -1,39 +0,0 @@ -process NANOSIM { - - tag "$meta.id" - label 'process_single' - - conda "bioconda::nanosim=3.1.0" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/nanosim:3.1.0--hdfd78af_0': - 'biocontainers/nanosim:3.1.0--hdfd78af_0' }" - - input: - tuple val(meta), path(fasta) - - output: - tuple val(meta), path("*.fastq.gz*"), emit: fastq - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def seed = task.ext.seed ?: "${meta.seed}" - """ - read_analysis.py metagenome \\ - --read ${fasta} \\ - --probe ${probes} \\ - --ID ${prefix} \\ - --seed ${seed} \\ - ${args2} ${prefix} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - nanosim: \$(read_analysis.py --version) - END_VERSIONS - """ -} diff --git a/modules/local/pbsim.nf b/modules/local/pbsim.nf deleted file mode 100644 index c8a941a..0000000 --- a/modules/local/pbsim.nf +++ /dev/null @@ -1,42 +0,0 @@ -process PBSIM { - - tag "$meta.id" - label 'process_single' - - // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. - conda "bioconda::pbsim=1.0.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/pbsim:1.0.3--h4ac6f70_7': - 'biocontainers/pbsim:1.0.3--h4ac6f70_7' }" - - input: - tuple val(meta), path(fasta) - path(model) - - output: - tuple val(meta), path("*.fastq.gz"), emit: fastq - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def seed = task.ext.seed ?: "${meta.seed}" - def VERSION = '1.0.3' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. - """ - pbsim \\ - --prefix $prefix \\ - --seed $seed \\ - $args $model \\ - $fasta - - gzip ${prefix}*.fastq - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - pbsim: $VERSION - END_VERSIONS - """ -} diff --git a/modules/local/simlord.nf b/modules/local/simlord.nf deleted file mode 100644 index 71c291f..0000000 --- a/modules/local/simlord.nf +++ /dev/null @@ -1,38 +0,0 @@ -process SIMLORD { - - tag "$meta.id" - label 'process_single' - - conda "bioconda::simlord=1.0.4" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/simlord:1.0.4--py310h4b81fae_3': - 'biocontainers/simlord:1.0.4--py310h4b81fae_3' }" - - input: - tuple val(meta), path(fasta) - - output: - tuple val(meta), path("*.fastq.gz"), emit: fastq - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def seed = task.ext.seed ?: "${meta.seed}" - """ - simlord \\ - --read-reference $fasta \\ - $args \\ - $prefix - - gzip ${prefix}.fastq - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - pbsim: \$(simlord --version) - END_VERSIONS - """ -} diff --git a/nextflow.config b/nextflow.config index 0ac2c82..a5fc9ba 100644 --- a/nextflow.config +++ b/nextflow.config @@ -9,7 +9,6 @@ // Global default params, used in configs params { - // TODO nf-core: Specify your pipeline's command line flags // Input options input = null // References @@ -17,20 +16,26 @@ params { //igenomes_base = 's3://ngi-igenomes/igenomes' igenomes_base = null igenomes_ignore = false - pbsim_model = 'https://raw.githubusercontent.com/a4000/test_data/main/models/model_qc_clr' - fasta = null - fw_primer = 'GTCGGTAAAACTCGTGCCAGC' - rv_primer = 'CATAGTGGGGTATCTAATCCCAGTTTG' // Simulation options amplicon = null target_capture = null - // Provider options - illumina = null - pacbio = null - nanopore = null + // Amplicon options + fw_primer = 'GTCGGTAAAACTCGTGCCAGC' + rv_primer = 'CATAGTGGGGTATCTAATCCCAGTTTG' + amplicon_ref_source = 'mitofish' + amplicon_ncbi_db = 'nucleotide' + amplicon_ncbi_query = '16S[All Fields] AND ("1"[SLEN] : "50000"[SLEN])' + amplicon_ncbi_species = 'Carcharinus leucas + Carcharinus acronotus' + amplicon_ncbi_email = 'johndoe@gmail.com' + amplicon_ncbi_batchsize = 5000 + amplicon_embl_db = 'vrt*' + amplicon_bold_db = 'Actinopterygii|Aves' + + // Target capture options + target_capture_pacbio = null // MultiQC options multiqc_config = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 15f6278..73e5535 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -40,13 +40,6 @@ "type": "string", "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", "fa_icon": "fas fa-file-signature" - }, - "pbsim_model": { - "type": "string", - "format": "file-path", - "exists": true, - "description": "Path to pbsim model.", - "fa_icon": "fas fa-file-signature" } } }, @@ -68,34 +61,11 @@ } } }, - "provider_options": { - "title": "Provider options", - "type": "object", - "fa_icon": "fas fa-terminal", - "description": "Choose the sequencing provider of the simulated reads.", - "properties": { - "illumina": { - "type": "boolean", - "description": "Option to simulate illumina data.", - "fa_icon": "fas fa-question-circle" - }, - "pacbio": { - "type": "boolean", - "description": "Option to simulate pacbio data.", - "fa_icon": "fas fa-question-circle" - }, - "nanopore": { - "type": "boolean", - "description": "Option to simulate nanopore data.", - "fa_icon": "fas fa-question-circle" - } - } - }, "amplicon_options": { "title": "Amplicon options", "type": "object", "fa_icon": "fas fa-dna", - "description": "Options required when simulating amplicon data.", + "description": "Options for simulating amplicon data.", "properties": { "fw_primer": { "type": "string", @@ -106,6 +76,58 @@ "type": "string", "description": "Reverse primer to use with crabs_insilicopcr. E.g., CATAGTGGGGTATCTAATCCCAGTTTG", "default": "CATAGTGGGGTATCTAATCCCAGTTTG" + }, + "amplicon_ref_source": { + "type": "string", + "description": "Source for downloading reference database when not using `--fasta` parameter. Can be 'ncbi', 'embl', 'bold', or 'mitofish'. Currently only ncbi and embl works", + "default": "mitofish" + }, + "amplicon_ncbi_db": { + "type": "string", + "description": "NCBI database to use when `--amplicon_ref_source` is set to 'ncbi'", + "default": "nucleotide" + }, + "amplicon_ncbi_query": { + "type": "string", + "description": "NCBI query to use when `--amplicon_ref_source` is set to 'ncbi'", + "default": "16S[All Fields] AND (\"1\"[SLEN] : \"50000\"[SLEN])" + }, + "amplicon_ncbi_species": { + "type": "string", + "description": "Species to use when `--amplicon_ref_source` is set to 'ncbi', seperate taxa with ' + '", + "default": "Carcharinus leucas + Carcharinus acronotus" + }, + "amplicon_ncbi_email": { + "type": "string", + "description": "email to conect to ncbi when `--amplicon_ref_source` is set to 'ncbi'", + "default": "johndoe@gmail.com" + }, + "amplicon_ncbi_batchsize": { + "type": "integer", + "description": "Batchsize to use when `--amplicon_ref_source` is set to 'ncbi'", + "default": 5000 + }, + "amplicon_embl_db": { + "type": "string", + "description": "EMBL database to use when `--amplicon_ref_source` is set to 'embl'", + "default": "vrt*" + }, + "amplicon_bold_db": { + "type": "string", + "description": "BOLD database to use when `--amplicon_ref_source` is set to 'bold', seperate databases with '|'", + "default": "Actinopterygii|Aves" + } + } + }, + "target_capture_options": { + "title": "Target capture options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Options for simulating target capture data.", + "properties": { + "target_capture_pacbio": { + "type": "boolean", + "description": "Use this option to simulate Pacbio long-read target capture reads. By default, only Illumina short-reads are simulated for target capture." } } }, @@ -128,7 +150,7 @@ "mimetype": "text/plain", "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", "description": "Path to FASTA genome file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "help_text": "This parameter is *mandatory* if `--genome` is not specified, unless simulating amplicon data. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", "fa_icon": "far fa-file-code" }, "igenomes_base": { diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index 407b137..c6e8716 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -2,13 +2,10 @@ // Simulate amplicon reads // +include { CRABS_DBDOWNLOAD } from '../../modules/local/crabs_dbdownload' include { CRABS_DBIMPORT } from '../../modules/local/crabs_dbimport' include { CRABS_INSILICOPCR } from '../../modules/local/crabs_insilicopcr' include { ART_ILLUMINA } from '../../modules/nf-core/art/illumina/main' -include { PBSIM } from '../../modules/local/pbsim' -include { SIMLORD } from '../../modules/local/simlord' -include { BADREAD } from '../../modules/local/badread' -include { NANOSIM } from '../../modules/local/nanosim' workflow AMPLICON_WORKFLOW { ch_versions = Channel.empty() @@ -18,86 +15,64 @@ workflow AMPLICON_WORKFLOW { ch_input // channel: [ meta ] main: - // Add a meta map to fasta channel for compatibility with modules - ch_meta_fasta = ch_fasta - .map { - fasta -> - def meta = [:] - meta.id = "illumina_amplicon" - return [ meta, fasta ] - } + ch_ref_fasta = Channel.empty() + if ( params.fasta ) { + CRABS_DBDOWNLOAD () + ch_versions = ch_versions.mix(CRABS_DBDOWNLOAD.out.versions.first()) + ch_ref_fasta = CRABS_DBDOWNLOAD.out.fasta + .map { + fasta -> + def meta = [:] + meta.id = "amplicon" + return [ meta, fasta ] + } - // - // MODULE: Run Crabs db_import - // - CRABS_DBIMPORT ( - ch_meta_fasta - ) - ch_versions = ch_versions.mix(CRABS_DBIMPORT.out.versions.first()) + } else { + ch_meta_fasta = Channel.fromPath(params.fasta) + .map { + fasta -> + def meta = [:] + meta.id = "amplicon" + return [ meta, fasta ] + } + + // + // MODULE: Run Crabs db_import + // + CRABS_DBIMPORT ( + ch_meta_fasta + ) + ch_versions = ch_versions.mix(CRABS_DBIMPORT.out.versions.first()) + ch_ref_fasta = CRABS_DBIMPORT.out.fasta + } // // MODULE: Run Crabs insilico_pcr // CRABS_INSILICOPCR ( - CRABS_DBIMPORT.out.fasta + ch_ref_fasta ) ch_versions = ch_versions.mix(CRABS_INSILICOPCR.out.versions.first()) // Now that we have processed our fasta file, // we need to map it to our sample data - ch_meta_fasta = CRABS_INSILICOPCR.out.fasta + ch_art_input = CRABS_INSILICOPCR.out.fasta .combine ( ch_input ) .map { it = [ it[2], it[1] ] } - //.map { - // meta, fasta -> - // meta.id = "illumina_amplicon_" + meta.id - // return [ meta, fasta ] - //} // // MODULE: Simulate Illumina reads // - ch_illumina_reads = Channel.empty() - if ( params.illumina ) { - ART_ILLUMINA ( - ch_meta_fasta, - "HS25", - 130 - ) - ch_versions = ch_versions.mix(ART_ILLUMINA.out.versions.first()) - ch_illumina_reads = ART_ILLUMINA.out.fastq - } - - // - // MODULE: Simulate pacbio reads - // - ch_pacbio_reads = Channel.empty() - if ( params.pacbio ) { - ch_model = Channel.fromPath(params.pbsim_model) - PBSIM ( - ch_meta_fasta, - ch_model.first() - ) - ch_versions = ch_versions.mix(PBSIM.out.versions.first()) - ch_pacbio_reads = PBSIM.out.fastq - //SIMLORD ( - // ch_meta_fasta - //) - //ch_pacbio_reads = SIMLORD.out.fastq - } - - //BADREAD ( - // ch_meta_fasta - //) - - //NANOSIM ( - // ch_meta_fasta - //) + ART_ILLUMINA ( + ch_art_input, + "HS25", + 130 + ) + ch_versions = ch_versions.mix(ART_ILLUMINA.out.versions.first()) emit: - illumina_reads = ch_illumina_reads // channel: [ meta, fastq ] - pacbio_reads = ch_pacbio_reads // channel: [ meta, fastq ] - versions = ch_versions // channel: [ versions.yml ] + reads = ART_ILLUMINA.out.fastq // channel: [ meta, fastq ] + versions = ch_versions // channel: [ versions.yml ] } diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index 3c993cb..5f79b12 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -22,7 +22,7 @@ workflow TARGET_CAPTURE_WORKFLOW { .map { fasta -> def meta = [:] - meta.id = "illumina_uce" + meta.id = "target_capture" return [ meta, fasta ] } @@ -68,29 +68,22 @@ workflow TARGET_CAPTURE_WORKFLOW { .map { it = [ it[3], it[0], it[1], it[2] ] } - //.map { - // meta, fasta, bam, index -> - // meta.id = "illumina_taget_capture_" + meta.id - // return [ meta, fasta, bam, index ] - //} // // MODULE: Simulate target capture illumina reads // ch_illumina_reads = Channel.empty() - if ( params.illumina ) { - CAPSIM_ILLUMINA ( - ch_capsim_input - ) - ch_versions = ch_versions.mix(CAPSIM_ILLUMINA.out.versions.first()) - ch_illumina_reads = CAPSIM_ILLUMINA.out.fastq - } + CAPSIM_ILLUMINA ( + ch_capsim_input + ) + ch_versions = ch_versions.mix(CAPSIM_ILLUMINA.out.versions.first()) + ch_illumina_reads = CAPSIM_ILLUMINA.out.fastq // // MODULE: Simulate target capture pacbio reads // ch_pacbio_reads = Channel.empty() - if ( params.pacbio ) { + if ( params.taget_capture_pacbio ) { CAPSIM_PACBIO ( ch_capsim_input ) @@ -100,6 +93,6 @@ workflow TARGET_CAPTURE_WORKFLOW { emit: illumina_reads = ch_illumina_reads // channel: [ meta, fastq ] - pacbio_reads = ch_pacbio_reads // channel: [ meta, fastq ] + pacbio_reads = ch_pacbio_reads // channel: [ meta, fastq ] versions = ch_versions // channel: [ versions.yml ] } diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 4ddf8ec..6937fac 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -76,7 +76,7 @@ workflow READSIMULATOR { ch_input ) ch_versions = ch_versions.mix(AMPLICON_WORKFLOW.out.versions.first()) - ch_fastq_input = ch_fastq_input.mix(AMPLICON_WORKFLOW.out.illumina_reads) + ch_fastq_input = ch_fastq_input.mix(AMPLICON_WORKFLOW.out.reads) } // From b8d41965c527d7e9502e192427bb8e2c34d366f6 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 4 Oct 2023 07:50:56 +0000 Subject: [PATCH 05/89] Added reference probes and improved documentation --- CITATIONS.md | 20 +++++ README.md | 16 ++-- assets/samplesheet.csv | 6 +- docs/output.md | 84 ++++++++++++++++++- docs/usage.md | 40 ++++----- lib/WorkflowReadsimulator.groovy | 2 +- nextflow.config | 8 +- nextflow_schema.json | 37 +++++++- subworkflows/local/amplicon_workflow.nf | 8 +- subworkflows/local/target_capture_workflow.nf | 16 +++- workflows/readsimulator.nf | 42 +++++----- 11 files changed, 206 insertions(+), 73 deletions(-) diff --git a/CITATIONS.md b/CITATIONS.md index 6b7717f..1250e1b 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -10,6 +10,22 @@ ## Pipeline tools +- [ART](https://www.niehs.nih.gov/research/resources/software/biostatistics/art/index.cfm) + + > Weichun Huang, Leping Li, Jason R. Myers, Gabor T. Marth, ART: a next-generation sequencing read simulator, Bioinformatics, Volume 28, Issue 4, February 2012, Pages 593–594, https://doi.org/10.1093/bioinformatics/btr708 + +- [Bowtie2](https://www.nature.com/articles/nmeth.1923) + + > Langmead, B., Salzberg, S. Fast gapped-read alignment with Bowtie 2. Nat Methods 9, 357–359 (2012). https://doi.org/10.1038/nmeth.1923 + +- [CapSim](https://academic.oup.com/bioinformatics/article/34/5/873/4575140) + + > Minh Duc Cao, Devika Ganesamoorthy, Chenxi Zhou, Lachlan J M Coin, Simulating the dynamics of targeted capture sequencing with CapSim, Bioinformatics, Volume 34, Issue 5, March 2018, Pages 873–874, https://doi.org/10.1093/bioinformatics/btx691 + +- [CRABS](https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13741) + + > Jeunen, G.-J., Dowle, E., Edgecombe, J., von Ammon, U., Gemmell, N. J., & Cross, H. (2022). crabs—A software program to generate curated reference databases for metabarcoding sequencing data. Molecular Ecology Resources, 00, 1– 14. https://doi.org/10.1111/1755-0998.13741 + - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. Available online https://www.bioinformatics.babraham.ac.uk/projects/fastqc/. @@ -18,6 +34,10 @@ > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. +- [Samtools](https://academic.oup.com/gigascience/article/10/2/giab008/6137722?login=false) + + > Twelve years of SAMtools and BCFtools. Petr Danecek, James K Bonfield, Jennifer Liddle, John Marshall, Valeriu Ohan, Martin O Pollard, Andrew Whitwham, Thomas Keane, Shane A McCarthy, Robert M Davies, Heng Li. GigaScience, Volume 10, Issue 2, February 2021, giab008, https://doi.org/10.1093/gigascience/giab008 + ## Software packaging/containerisation tools - [Anaconda](https://anaconda.com) diff --git a/README.md b/README.md index 8019e4d..201d37d 100644 --- a/README.md +++ b/README.md @@ -24,8 +24,8 @@ workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples. --> -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) +1. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +2. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) ## Usage @@ -34,30 +34,26 @@ > to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) > with `-profile test` before running the workflow on actual data. - Now, you can run the pipeline using: - - ```bash nextflow run nf-core/readsimulator \ -profile \ --input samplesheet.csv \ + --amplicon \ --outdir ``` diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 5f653ab..a359015 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,3 +1,3 @@ -sample,fastq_1,fastq_2 -SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz -SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz, +sample,seed +SAMPLE_1,41 +SAMPLE_2,42 diff --git a/docs/output.md b/docs/output.md index 31a4766..152621e 100644 --- a/docs/output.md +++ b/docs/output.md @@ -12,9 +12,75 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -- [FastQC](#fastqc) - Raw read QC -- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline -- [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution + - [ART](#art) - ART Illumina simulated reads + - [Bowtie2](#bowtie2) - Bowtie2 alignment + - [CapSim](#capsim) - CapSim simulated reads (Illumina or Pacbio) + - [CRABS](#crabs) - Formats reference databases for simulating amplicon reads + - [FastQC](#fastqc) - Raw read QC + - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline + - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution + - [SAMtools](#samtools) - SAMtools index + +### ART + +
+Output files + +- `art_illumina/` + - `*1.fq.gz`: Read 1 files simulating Illumina reads. The prefix will be the sample name found in the samplesheet. + - `*2.fq.gz`: Read 2 files simulating Illumina reads. The prefix will be the sample name found in the samplesheet. + +
+ +[ART](https://www.niehs.nih.gov/research/resources/software/biostatistics/art/index.cfm) is a tool for simulating Illumina sequencing reads. For further reading and documentation see the [ART Illumina manual](https://manpages.debian.org/testing/art-nextgen-simulation-tools/art_illumina.1.en.html). + +### Bowtie2 + +
+Output files + +- `bowtie2/` + - `bowtie2/` + - `*.bt2`: Bowtie2 index files. + - `*.bam`: BAM file produced from aligning with Bowtie2. + - `*.bowtie2.log`: Log file containing alignment information. + +
+ +[Bowtie2](https://www.nature.com/articles/nmeth.1923) is a popular tool for aligning sequences to reference reads. For further reading and documentation see the [Bowtie2 manual](https://bowtie-bio.sourceforge.net/bowtie2/manual.shtml). + +### CapSim + +
+Output files + +- `capsim_illumina/` + - `*_1.fastq.gz`: Read 1 files simulating Illumina reads. The prefix will be the sample name found in the samplesheet. + - `*_2.fastq.gz`: Read 2 files simulating Illumina reads. The prefix will be the sample name found in the samplesheet. +- `capsim_pacbio/` + - `*_1.fastq.gz`: Read 1 files simulating Pacbio reads. The prefix will be the sample name found in the samplesheet. + - `*_1.fastq.gz`: Read 2 files simulating Pacbio reads. The prefix will be the sample name found in the samplesheet. + + +
+ +[CapSim](https://academic.oup.com/bioinformatics/article/34/5/873/4575140) is a tool to simulate capture sequencing reads. It's part of the Japsa package. For further reading and documentation see the [CapSim documentation](https://japsa.readthedocs.io/en/latest/tools/jsa.sim.capsim.html). + +### CRABS + +
+Output files + +- `crabs_dbdownload/` + - `*.fasta`: Reference fasta file. +- `crabs_dbimport/` + - `*.fa`: Reference fasta file. +- `crabs_insilicopcr/` + - `*.fa`: Reference fasta file for simulating amplicon data. + +
+ +[CRABS](https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.13741) is a toolfor reformating reference databases for simulating amplicon sequencing data. For further reading and documentation see the [CRABS repo](https://github.com/gjeunen/reference_database_creator). ### FastQC @@ -66,3 +132,15 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ [Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. + +### SAMtools + +
+Output files + +- `samtools/` + - `*.bai`: Index file produced with SAMtools. + +
+ +[SAMtools](https://academic.oup.com/gigascience/article/10/2/giab008/6137722?login=false) is a popular set of tools for working with sequencing data. For further reading and documentation see the [SAMtools documentation](http://www.htslib.org/doc/). diff --git a/docs/usage.md b/docs/usage.md index 8596ff1..084efc8 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -10,45 +10,32 @@ ## Samplesheet input -You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. +You will need to create a samplesheet with information about the samples you would like to simulate before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 2 columns, and a header row as shown in the examples below. ```bash --input '[path to samplesheet file]' ``` -### Multiple runs of the same sample - -The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: - -```console -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz -``` - ### Full samplesheet -The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. +The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 2 columns to match those defined in the table below. -A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. +A final samplesheet file may look something like the one below. This is for 6 samples. ```console -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz -CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz -TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, -TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, +sample,seed +sample_1,1 +sample_2,2 +sample_3,3 +sample_4,4 +sample_5,5 +sample_6,6 ``` | Column | Description | | --------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `sample` | Custom sample name. This entry will be used as the prefix for many output files. Spaces in sample names are automatically converted to underscores (`_`). | +| `seed` | Seed used with tools that have a seed parameter for Random Number Generation. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. @@ -57,7 +44,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/readsimulator --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker +nextflow run nf-core/readsimulator --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker <--amplicon | --target_capture> ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -89,6 +76,7 @@ with `params.yaml` containing: input: './samplesheet.csv' outdir: './results/' genome: 'GRCh37' +target_capture: true <...> ``` diff --git a/lib/WorkflowReadsimulator.groovy b/lib/WorkflowReadsimulator.groovy index 3c3a765..b822c3d 100755 --- a/lib/WorkflowReadsimulator.groovy +++ b/lib/WorkflowReadsimulator.groovy @@ -14,7 +14,7 @@ class WorkflowReadsimulator { genomeExistsError(params, log) - if (!params.fasta) { + if (!params.fasta && !params.amplicon) { Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." } } diff --git a/nextflow.config b/nextflow.config index a5fc9ba..f2636ac 100644 --- a/nextflow.config +++ b/nextflow.config @@ -25,7 +25,7 @@ params { // Amplicon options fw_primer = 'GTCGGTAAAACTCGTGCCAGC' rv_primer = 'CATAGTGGGGTATCTAATCCCAGTTTG' - amplicon_ref_source = 'mitofish' + amplicon_ref_source = 'bold' amplicon_ncbi_db = 'nucleotide' amplicon_ncbi_query = '16S[All Fields] AND ("1"[SLEN] : "50000"[SLEN])' amplicon_ncbi_species = 'Carcharinus leucas + Carcharinus acronotus' @@ -36,6 +36,8 @@ params { // Target capture options target_capture_pacbio = null + probe_fasta = null + probe_ref_name = 'Tetrapods-UCE-5Kv1' // MultiQC options multiqc_config = null @@ -54,6 +56,7 @@ params { hook_url = null help = false version = false + schema_ignore_params = 'probe_ref_db' // Config options config_profile_name = null @@ -82,6 +85,9 @@ params { // Load base.config by default for all pipelines includeConfig 'conf/base.config' +// Load ref_databases.config for reference taxonomy +includeConfig 'conf/ref_databases.config' + // Load nf-core custom profiles from different Institutions try { includeConfig "${params.custom_config_base}/nfcore_custom.config" diff --git a/nextflow_schema.json b/nextflow_schema.json index 73e5535..cc38d36 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -79,8 +79,9 @@ }, "amplicon_ref_source": { "type": "string", - "description": "Source for downloading reference database when not using `--fasta` parameter. Can be 'ncbi', 'embl', 'bold', or 'mitofish'. Currently only ncbi and embl works", - "default": "mitofish" + "description": "Source for downloading reference database when using `--amplicon` parameter, but not using `--fasta` parameter.", + "help_text": "Can be 'ncbi', 'embl', 'bold', or 'mitofish'. Currently only ncbi and bold works.", + "default": "bold" }, "amplicon_ncbi_db": { "type": "string", @@ -127,7 +128,37 @@ "properties": { "target_capture_pacbio": { "type": "boolean", - "description": "Use this option to simulate Pacbio long-read target capture reads. By default, only Illumina short-reads are simulated for target capture." + "description": "Use this option to simulate Pacbio long-read target capture reads.", + "help_text": "By default, only Illumina short-reads are simulated for target capture data." + }, + "probe_fasta": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/plain", + "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", + "description": "Path to FASTA probe file.", + "help_text": "This parameter is *mandatory* if `--probe_ref_name` is not specified but `--target_capture` is specified.", + "fa_icon": "far fa-file-code" + }, + "probe_ref_name": { + "type": "string", + "default": "Tetrapods-UCE-5Kv1", + "description": "Name of supported probe. Mandatory if not using `--probes` parameter.", + "help_text": "Supported probes are 'Tetrapods-UCE-2.5Kv1', 'Tetrapods-UCE-5Kv1', 'Actinopterygians-0.5Kv1', 'Acanthomorphs-1Kv1', 'Arachnida-1.1Kv1', 'Coleoptera-1.1Kv1', 'Diptera-2.7Kv1', 'Hemiptera-2.7Kv1', 'Hymenoptera-1.5Kv1', 'Hymenoptera-2.5Kv2', and 'Anthozoa-1.7Kv1'", + "enum": [ + "Tetrapods-UCE-2.5Kv1", + "Tetrapods-UCE-5Kv1", + "Actinopterygians-0.5Kv1", + "Acanthomorphs-1Kv1", + "Arachnida-1.1Kv1", + "Coleoptera-1.1Kv1", + "Diptera-2.7Kv1", + "Hemiptera-2.7Kv1", + "Hymenoptera-1.5Kv1", + "Hymenoptera-2.5Kv2", + "Anthozoa-1.7Kv1" + ] } } }, diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index c6e8716..75c3353 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -16,9 +16,9 @@ workflow AMPLICON_WORKFLOW { main: ch_ref_fasta = Channel.empty() - if ( params.fasta ) { + if ( !params.fasta ) { CRABS_DBDOWNLOAD () - ch_versions = ch_versions.mix(CRABS_DBDOWNLOAD.out.versions.first()) + ch_versions = ch_versions.mix(CRABS_DBDOWNLOAD.out.versions) ch_ref_fasta = CRABS_DBDOWNLOAD.out.fasta .map { fasta -> @@ -42,7 +42,7 @@ workflow AMPLICON_WORKFLOW { CRABS_DBIMPORT ( ch_meta_fasta ) - ch_versions = ch_versions.mix(CRABS_DBIMPORT.out.versions.first()) + ch_versions = ch_versions.mix(CRABS_DBIMPORT.out.versions) ch_ref_fasta = CRABS_DBIMPORT.out.fasta } @@ -52,7 +52,7 @@ workflow AMPLICON_WORKFLOW { CRABS_INSILICOPCR ( ch_ref_fasta ) - ch_versions = ch_versions.mix(CRABS_INSILICOPCR.out.versions.first()) + ch_versions = ch_versions.mix(CRABS_INSILICOPCR.out.versions) // Now that we have processed our fasta file, // we need to map it to our sample data diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index 5f79b12..6250e8b 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -7,6 +7,7 @@ include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/ include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' include { CAPSIM as CAPSIM_ILLUMINA } from '../../modules/local/capsim' include { CAPSIM as CAPSIM_PACBIO } from '../../modules/local/capsim' +include { UNZIP } from '../../modules/local/unzip' workflow TARGET_CAPTURE_WORKFLOW { take: @@ -34,12 +35,21 @@ workflow TARGET_CAPTURE_WORKFLOW { ) ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions.first()) + if ( !params.probe_fasta ) { + ch_zip_file = Channel.fromPath(params.probe_ref_db[params.probe_ref_name]["url"]) + ch_probes = UNZIP ( + ch_zip_file + ).file + } ch_probes = ch_probes .map { - meta, fasta -> + fasta -> + def meta = [:] + meta.id = "probes" meta.single_end = true - return [ meta, fasta ] + [ meta, fasta ] } + // // MODULE: Align probes to genome // @@ -83,7 +93,7 @@ workflow TARGET_CAPTURE_WORKFLOW { // MODULE: Simulate target capture pacbio reads // ch_pacbio_reads = Channel.empty() - if ( params.taget_capture_pacbio ) { + if ( params.target_capture_pacbio ) { CAPSIM_PACBIO ( ch_capsim_input ) diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 6937fac..50a9168 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -62,42 +62,46 @@ def multiqc_report = [] workflow READSIMULATOR { - ch_versions = Channel.empty() - ch_fasta = Channel.fromPath(params.fasta) - ch_input = Channel.fromSamplesheet("input") - ch_fastq_input = Channel.empty() + ch_versions = Channel.empty() + ch_input = Channel.fromSamplesheet("input") + ch_fastqc_input = Channel.empty() + + if ( params.fasta ) { + ch_fasta = Channel.fromPath(params.fasta) + } else { + ch_fasta = Channel.empty() + } + + if ( params.probe_fasta ) { + ch_probes = Channel.fromPath(params.probe_fasta) + } else { + ch_probes = Channel.empty() + } // // SUBWORKFLOW: Simulate amplicon reads // if ( params.amplicon ) { AMPLICON_WORKFLOW ( - ch_fasta, + ch_fasta.ifEmpty([]), ch_input ) - ch_versions = ch_versions.mix(AMPLICON_WORKFLOW.out.versions.first()) - ch_fastq_input = ch_fastq_input.mix(AMPLICON_WORKFLOW.out.reads) + ch_versions = ch_versions.mix(AMPLICON_WORKFLOW.out.versions.first()) + ch_fastqc_input = ch_fastqc_input.mix(AMPLICON_WORKFLOW.out.reads) } // // SUBWORKFLOW: Simulate UCE taget capture reads // if ( params.target_capture ) { - ch_probes = Channel.fromPath(params.probes) - ch_probes = ch_probes.map { - fasta -> - def meta = [:] - meta.id = "probes" - [ meta, fasta ] - } TARGET_CAPTURE_WORKFLOW ( ch_fasta, ch_input, - ch_probes + ch_probes.ifEmpty([]) ) - ch_versions = ch_versions.mix(TARGET_CAPTURE_WORKFLOW.out.versions.first()) - ch_fastq_input = ch_fastq_input.mix(TARGET_CAPTURE_WORKFLOW.out.illumina_reads) - ch_fastq_input = ch_fastq_input.mix(TARGET_CAPTURE_WORKFLOW.out.pacbio_reads) + ch_versions = ch_versions.mix(TARGET_CAPTURE_WORKFLOW.out.versions.first()) + ch_fastqc_input = ch_fastqc_input.mix(TARGET_CAPTURE_WORKFLOW.out.illumina_reads) + ch_fastqc_input = ch_fastqc_input.mix(TARGET_CAPTURE_WORKFLOW.out.pacbio_reads) } // MODULE: Create sample sheet @@ -107,7 +111,7 @@ workflow READSIMULATOR { // MODULE: Run FastQC // FASTQC ( - ch_fastq_input + ch_fastqc_input ) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) From fee6188d1574cb9fe578fd665df3fc3f174996bc Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Fri, 6 Oct 2023 06:39:02 +0000 Subject: [PATCH 06/89] added samplesheet creation, metagenome read simulation and improvements to documentation --- CHANGELOG.md | 5 +- CITATIONS.md | 4 + README.md | 13 +-- conf/base.config | 1 - conf/modules.config | 76 +++++++++---- conf/ref_databases.config | 68 +++++++++++ docs/output.md | 45 +++++++- docs/usage.md | 4 +- lib/WorkflowReadsimulator.groovy | 41 ++++--- modules/local/capsim.nf | 42 ------- modules/local/crabs_dbdownload.nf | 5 +- modules/local/crabs_dbimport.nf | 2 +- modules/local/crabs_insilicopcr.nf | 2 +- modules/local/create_samplesheet.nf | 35 ++++++ modules/local/insilicoseq_generate.nf | 64 +++++++++++ modules/local/japsa_capsim.nf | 40 +++++++ modules/local/merge_samplesheets.nf | 30 +++++ modules/local/unzip.nf | 31 +++++ nextflow.config | 21 +++- nextflow_schema.json | 107 +++++++++++++++++- subworkflows/local/amplicon_workflow.nf | 22 +++- subworkflows/local/target_capture_workflow.nf | 28 +++-- workflows/readsimulator.nf | 71 ++++++++++-- 23 files changed, 630 insertions(+), 127 deletions(-) create mode 100644 conf/ref_databases.config delete mode 100644 modules/local/capsim.nf create mode 100644 modules/local/create_samplesheet.nf create mode 100644 modules/local/insilicoseq_generate.nf create mode 100644 modules/local/japsa_capsim.nf create mode 100644 modules/local/merge_samplesheets.nf create mode 100644 modules/local/unzip.nf diff --git a/CHANGELOG.md b/CHANGELOG.md index 0e7b4cb..df450a9 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,7 +8,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 Initial release of nf-core/readsimulator, created with the [nf-core](https://nf-co.re/) template. ### `Added` -Subworkflow to simulate illumina amplicon data +Option to simulate amplicon sequencing reads +Option to simulate metagenomic sequencing reads +Option to simulate target capture sequencing reads +Samplesheet creation after reads have been simulated ### `Fixed` diff --git a/CITATIONS.md b/CITATIONS.md index 1250e1b..4fad81b 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -30,6 +30,10 @@ > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. Available online https://www.bioinformatics.babraham.ac.uk/projects/fastqc/. +- [InSilicoSeq](https://academic.oup.com/bioinformatics/article/35/3/521/5055123) + + > Gourlé H, Karlsson-Lindsjö O, Hayer J and Bongcam+Rudloff E, Simulating Illumina data with InSilicoSeq. Bioinformatics (2018) doi:10.1093/bioinformatics/bty630 + - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. diff --git a/README.md b/README.md index 201d37d..593350f 100644 --- a/README.md +++ b/README.md @@ -14,11 +14,7 @@ **nf-core/readsimulator** is a bioinformatics pipeline that ... - +nf-core/readsimulator is a pipeline to simulate sequencing reads. The pipeline currently supports simulating Amplicon, Metagenomic, and Target Capture data. It takes a samplesheet with sample names and seeds for random generation to produce simulated fastq files and a samplesheet that contains the paths to the fastq files. @@ -72,11 +68,12 @@ For more details about the output files and reports, please refer to the ## Credits -nf-core/readsimulator was originally written by Adam Bennett. +nf-core/readsimulator was originally written by Adam Bennett ([@a4000](https://github.com/a4000)) for use at the [MinderooFoundation](https://www.minderoo.org/). -We thank the following people for their extensive assistance in the development of this pipeline: +We thank the following people for their extensive assistance in the development of this pipeline (in alphabetical order): - +- [Lauren Huet](https://github.com/LaurenHuet/) +- [Philipp Bayer](https://github.com/philippbayer) ## Contributions and Support diff --git a/conf/base.config b/conf/base.config index 43abe99..081c2eb 100644 --- a/conf/base.config +++ b/conf/base.config @@ -24,7 +24,6 @@ process { // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. // If possible, it would be nice to keep the same label naming convention when // adding in your local modules too. - // TODO nf-core: Customise requirements for specific processes. // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_single { cpus = { check_max( 1 , 'cpus' ) } diff --git a/conf/modules.config b/conf/modules.config index 857cada..05c7945 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,9 +18,32 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] + withName: ART_ILLUMINA { + ext.args = "-amp -p -na -c 500" + publishDir = [ + path: { "${params.outdir}/art_illumina" }, + mode: params.publish_dir_mode + ] + } + + withName: BOWTIE2_ALIGN { + ext.args = "--local --very-sensitive-local --mp 32 --rdg 10,8 --rfg 10,8 -k 10000 -f" + ext.args2 = "-b | samtools sort" + publishDir = [ + path: { "${params.outdir}/bowtie2" }, + mode: params.publish_dir_mode + ] + } + + withName: BOWTIE2_BUILD { + publishDir = [ + path: { "${params.outdir}/bowtie2" }, + mode: params.publish_dir_mode + ] + } + withName: CAPSIM_ILLUMINA { - ext.args = "--fmedian 500 --num 500000 --illen 150" - ext.args2 = "--miseq" + ext.args = "--fmedian 500 --num 500000 --illen 150 --miseq" publishDir = [ path: { "${params.outdir}/capsim_illumina" }, mode: params.publish_dir_mode @@ -28,8 +51,7 @@ process { } withName: CAPSIM_PACBIO { - ext.args = "--fmedian 500 --num 500000" - ext.args2 = "--pacbio" + ext.args = "--fmedian 500 --num 500000 --pacbio" publishDir = [ path: { "${params.outdir}/capsim_pacbio" }, mode: params.publish_dir_mode @@ -68,40 +90,52 @@ process { ] } - withName: ART_ILLUMINA { - ext.args = "-amp -p -na -c 500" + withName: CREATE_SAMPLESHEET { publishDir = [ - path: { "${params.outdir}/art_illumina" }, + path: { "${params.outdir}/samplesheet_individual_samples" }, mode: params.publish_dir_mode ] } - withName: BOWTIE2_ALIGN { - ext.args = "--local --very-sensitive-local --mp 32 --rdg 10,8 --rfg 10,8 -k 10000 -f" - ext.args2 = "-b | samtools sort" + withName: CUSTOM_DUMPSOFTWAREVERSIONS { publishDir = [ - path: { "${params.outdir}/bowtie2" }, - mode: params.publish_dir_mode + path: { "${params.outdir}/pipeline_info" }, + mode: params.publish_dir_mode, + pattern: '*_versions.yml' ] } - withName: BOWTIE2_BUILD { + withName: FASTQC { + ext.args = '--quiet' + } + + withName: INSILICOSEQ_GENERATE { + ext.args = [ + "--abundance ${params.metagenome_abundance}", + "--n_reads ${params.metagenome_n_reads}", + "--mode ${params.metagenome_mode}", + params.metagenome_mode == "basic" ? "" : "--model ${params.metagenome_model}", + params.metagenome_coverage ? "--coverage ${params.metagenome_coverage}" : "", + params.metagenome_gc_bias ? "--gc_bias ${params.metagenome_gc_bias}" : "" + ].join(' ').trim() + ext.args2 = "${params.metagenome_ncbi_db} --n_genomes_ncbi ${params.metagenome_n_genomes}" publishDir = [ - path: { "${params.outdir}/bowtie2" }, + path: { "${params.outdir}/insilicoseq" }, mode: params.publish_dir_mode ] } - withName: FASTQC { - ext.args = '--quiet' + withName: MERGE_SAMPLESHEETS { + publishDir = [ + path: { "${params.outdir}/samplesheet" }, + mode: params.publish_dir_mode + ] } - withName: CUSTOM_DUMPSOFTWAREVERSIONS { + withName: UNZIP { publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - pattern: '*_versions.yml' + path: { "${params.outdir}/probes" }, + mode: params.publish_dir_mode ] } - } diff --git a/conf/ref_databases.config b/conf/ref_databases.config new file mode 100644 index 0000000..f7d368d --- /dev/null +++ b/conf/ref_databases.config @@ -0,0 +1,68 @@ +/* + * ----------------------------------------------------------- + * Nextflow config file for reference databases + * ----------------------------------------------------------- + * Defines sources and files for reference databases + * Please also reflect all changes in 'nextflow_schema.json' + * Each entry requires as a minimum: title, url, citation + */ + + params { + probe_ref_db { + 'Tetrapods-UCE-2.5Kv1' { + title = 'Tetrapods; 2,560 baits for 2,386 UCEs; version 1' + url = 'https://ndownloader.figshare.com/files/10513876' + citation = 'Brant C. Faircloth, John E. McCormack, Nicholas G. Crawford, Michael G. Harvey, Robb T. Brumfield, Travis C. Glenn, Ultraconserved Elements Anchor Thousands of Genetic Markers Spanning Multiple Evolutionary Timescales, Systematic Biology, Volume 61, Issue 5, October 2012, Pages 717–726, https://doi.org/10.1093/sysbio/sys004' + } + 'Tetrapods-UCE-5Kv1' { + title = 'Tetrapods; 5,472 baits for 5,060 UCEs; version 1' + url = 'https://ndownloader.figshare.com/files/10513882' + citation = 'Sun Keping, Meiklejohn Kelly A., Faircloth Brant C., Glenn Travis C., Braun Edward L. and Kimball Rebecca T., 2014 The evolution of peafowl and other taxa with ocelli (eyespots): a phylogenomic approach. Proc. R. Soc. B. 281: 20140823. 20140823. http://doi.org/10.1098/rspb.2014.0823' + } + 'Actinopterygians-0.5Kv1' { + title = 'Actinopterygians; 2,001 baits for 500 UCEs; version 1' + url = 'https://ndownloader.figshare.com/files/10513885' + citation = 'Faircloth BC, Sorenson L, Santini F, Alfaro ME (2013) A Phylogenomic Perspective on the Radiation of Ray-Finned Fishes Based upon Targeted Sequencing of Ultraconserved Elements (UCEs). PLOS ONE 8(6): e65923. https://doi.org/10.1371/journal.pone.0065923' + } + 'Acanthomorphs-1Kv1' { + title = 'Acanthomorphs; 2,628 baits for 1,314 UCEs; version 1' + url = 'https://ndownloader.figshare.com/files/11188235' + citation = 'McGee Matthew D., Faircloth Brant C., Borstein Samuel R., Zheng Jimmy, Darrin Hulsey C., Wainwright Peter C. and Alfaro Michael E.. 2016 Replicated divergence in cichlid radiations mirrors a major vertebrate innovation. Proc. R. Soc. B. 283: 20151413. 20151413. http://doi.org/10.1098/rspb.2015.1413' + } + 'Arachnida-1.1Kv1' { + title = 'Arachnida; 14,799 baits for 1,120 UCEs; version 1' + url = 'https://ndownloader.figshare.com/files/6042078' + citation = 'Starrett, J., Derkarabetian, S., Hedin, M., Bryson Jr. R. W., McCormack. J. E., & Faircloth. B. C. (2016). High phylogenetic utility of an Ultraconserved element probe set designed for Arachnida. Molecular Ecology Resources. 17(4), 812-823. https://doi.org/10.1111/1755-0998.12621' + } + 'Coleoptera-1.1Kv1' { + title = 'Coleoptera; 13,674 baits for 1,172 UCEs; version 1' + url = 'https://ndownloader.figshare.com/files/6042081' + citation = 'Baca. S. M., Alexander. A., Gustafson. G. T., & Short. A. E. Z. (2017). Ultraconserved elements show utility in phylogenetic inference of Adephaga (Coleoptera) and suggest paraphyly of Hydradephaga. 42(4), 786-795. https://doi.org/10.1111/syen.12244' + } + 'Diptera-2.7Kv1' { + title = 'Diptera; 31,328 baits for 2,711 UCEs; version 1' + url = 'https://ndownloader.figshare.com/files/6042084' + citation = 'Faircloth. B. C. (2017). Identifying conserved genomic elements and designing universal bait sets to enrich them. Methods in Ecology and Evolution. 8(9), 1103-1112. https://doi.org/10.1111/2041-210X.12754' + } + 'Hemiptera-2.7Kv1' { + title = 'Hemiptera; 40,207 baits for 2,731 UCEs; version 1' + url = 'https://ndownloader.figshare.com/files/6042087' + citation = 'Faircloth. B. C. (2017). Identifying conserved genomic elements and designing universal bait sets to enrich them. Methods in Ecology and Evolution. 8(9), 1103-1112. https://doi.org/10.1111/2041-210X.12754' + } + 'Hymenoptera-1.5Kv1' { + title = 'Hymenoptera; 2,749 baits for 1,510 UCEs; version 1' + url = 'https://ndownloader.figshare.com/files/10513873' + citation = 'Faircloth. B. C., Branstetter. M. G., White. N. D., & Brady. S. G. (2014). Target enrichment of ultraconserved elements from anthropods provides a genomic perspective on elationships among Hymenoptera. Molecular Ecology Resources. 15(3), 489-501. https://doi.org/10.1111/1755-0998.12328' + } + 'Hymenoptera-2.5Kv2' { + title = 'Hymenoptera; 31,829 baits for 2,590 UCEs; version 2' + url = 'https://ndownloader.figshare.com/files/7539658' + citation = 'Branstetter. M. G., Longino. J. T., Ward. P. S., & Faircloth. B. C. (2017). Enriching the ant tree of life: enhanced UCE bait set for genome-scale phylogenetics of ants and other Hymenoptera. 8(6), 768-776. https://doi.org/10.1111.2041-210X.12742' + } + 'Anthozoa-1.7Kv1' { + title = 'Anthozoa; 16,306 baits for 720 UCEs and 1,071 exons; version 1' + url = 'https://ndownloader.figshare.com/files/10513894' + citation = 'Quattrini. A. M., Faircloth. B. C., Duenas. L. F., Bridge. T. C. L., Brugler. M. R., Calixto-Botia. I. F., DeLeo. D. M., Foret. S., Herrera. S., Lee. S. M. Y., Miller. D. J., Prada. C., Radis-Baptista. G., Ramirez-Portilla. C., Sanchez. J. A., Rodriguez. E., & McFadden. C. S. (2017). Universal target-enrichment baits for anthozoan (Cnidaria) phylogenomics: New approaches to long-standing problems. Molecular Ecology Resources. 18(2), 281-295. https://doi.org/10.1111/1755-0998.12736' + } + } + } diff --git a/docs/output.md b/docs/output.md index 152621e..b338943 100644 --- a/docs/output.md +++ b/docs/output.md @@ -6,20 +6,21 @@ This document describes the output produced by the pipeline. Most of the plots a The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. - - ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: - [ART](#art) - ART Illumina simulated reads - - [Bowtie2](#bowtie2) - Bowtie2 alignment + - [Bowtie2](#bowtie2) - Bowtie2 alignments - [CapSim](#capsim) - CapSim simulated reads (Illumina or Pacbio) - - [CRABS](#crabs) - Formats reference databases for simulating amplicon reads + - [CRABS](#crabs) - Formated reference database for simulating amplicon reads - [FastQC](#fastqc) - Raw read QC + - [InSilicoSeq](#insilicoseq) - InSilicoSeq simulated metagenomic reads - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution + - [Samplesheet](#samplesheet) - Samplesheets produced during the running of the pipeline - [SAMtools](#samtools) - SAMtools index + - [Unzip](#unzip) - Unziped probe file ### ART @@ -103,6 +104,19 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d > **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. +### InSilicoSeq + +
+Output files + +- `insilicoseq/` + - `*R1.fastq.gz`: Read 1 files simulating Illumina metagenomic reads. The prefix will be the sample name found in the samplesheet. + - `*R2.fastq.gz`: Read 2 files simulating Illumina metagenomic reads. The prefix will be the sample name found in the samplesheet. + +
+ +[InSilicoSeq](https://academic.oup.com/bioinformatics/article/35/3/521/5055123) is a tool for simulating Illumina metgenomic sequencing reads. For further reading and documentation see the [InSilicoSeq documentation](https://insilicoseq.readthedocs.io/en/latest/). + ### MultiQC
@@ -133,6 +147,18 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ [Nextflow](https://www.nextflow.io/docs/latest/tracing.html) provides excellent functionality for generating various reports relevant to the running and execution of the pipeline. This will allow you to troubleshoot errors with the running of the pipeline, and also provide you with other information such as launch commands, run times and resource usage. +### Samplesheet + +
+Output files + +- `samplesheet/` + - `*.csv`: Samplesheets with all samples. +- `samplesheet_individual_samples/` + - `*.csv`: Samplesheets for each individual sample. + +
+ ### SAMtools
@@ -144,3 +170,14 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ
[SAMtools](https://academic.oup.com/gigascience/article/10/2/giab008/6137722?login=false) is a popular set of tools for working with sequencing data. For further reading and documentation see the [SAMtools documentation](http://www.htslib.org/doc/). + +### Unzip + +
+Output files + +- `probes/` + - `unziped/` + - `*.fasta`: Probe file downloaded if custom probe hasn't been provided with `--probe_fasta` parameter. + +
diff --git a/docs/usage.md b/docs/usage.md index 084efc8..a78b82c 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -6,7 +6,7 @@ ## Introduction - +The pipeline curently support simulating amplicon, metagenomic, and target capture sequencing reads. The different modes can be selected with `--amplicon`, `--metagenome`, or `--target_capture` respectively. ## Samplesheet input @@ -44,7 +44,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/readsimulator --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker <--amplicon | --target_capture> +nextflow run nf-core/readsimulator --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker <--amplicon/--target_capture/--metgenome> ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. diff --git a/lib/WorkflowReadsimulator.groovy b/lib/WorkflowReadsimulator.groovy index b822c3d..b27d813 100755 --- a/lib/WorkflowReadsimulator.groovy +++ b/lib/WorkflowReadsimulator.groovy @@ -14,9 +14,10 @@ class WorkflowReadsimulator { genomeExistsError(params, log) - if (!params.fasta && !params.amplicon) { + if (!params.fasta && params.target_capture) { Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." } + } // @@ -51,12 +52,19 @@ class WorkflowReadsimulator { // public static String toolCitationText(params) { - - // TODO Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report def citation_text = [ "Tools used in the workflow included:", + params["amplicon"] ? [ + "CRABS (Jeunen et al. 2022),", + "ART (Huang et al. 2012)," + ].join(' ').trim() : "", + params["target_capture"] ? [ + "Bowtie2 (Langmead et al. 2012),", + "Samtools (Danecek et al. 2021),", + "CapSim (Cao et al. 2018)," + ].join(' ').trim() : "", + params["metagenome"] ? + "InSilicoSeq (Gourlé et al. 2018)," : "", "FastQC (Andrews 2010),", "MultiQC (Ewels et al. 2016)", "." @@ -66,11 +74,18 @@ class WorkflowReadsimulator { } public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report def reference_text = [ + params["amplicon"] ? [ + "
  • Jeunen, G.-J., Dowle, E., Edgecombe, J., von Ammon, U., Gemmell, N. J., & Cross, H. (2022). crabs—A software program to generate curated reference databases for metabarcoding sequencing data. Molecular Ecology Resources, 00, 1– 14. https://doi.org/10.1111/1755-0998.13741
    • ", + "
  • Weichun Huang, Leping Li, Jason R. Myers, Gabor T. Marth, ART: a next-generation sequencing read simulator, Bioinformatics, Volume 28, Issue 4, February 2012, Pages 593–594, https://doi.org/10.1093/bioinformatics/btr708
    • " + ].join(' ').trim() : "", + params["target_capture"] ? [ + "
  • Langmead, B., Salzberg, S. Fast gapped-read alignment with Bowtie 2. Nat Methods 9, 357–359 (2012). https://doi.org/10.1038/nmeth.1923
    • ", + "
  • Twelve years of SAMtools and BCFtools. Petr Danecek, James K Bonfield, Jennifer Liddle, John Marshall, Valeriu Ohan, Martin O Pollard, Andrew Whitwham, Thomas Keane, Shane A McCarthy, Robert M Davies, Heng Li. GigaScience, Volume 10, Issue 2, February 2021, giab008, https://doi.org/10.1093/gigascience/giab008
    • ", + "
  • Minh Duc Cao, Devika Ganesamoorthy, Chenxi Zhou, Lachlan J M Coin, Simulating the dynamics of targeted capture sequencing with CapSim, Bioinformatics, Volume 34, Issue 5, March 2018, Pages 873–874, https://doi.org/10.1093/bioinformatics/btx691
    • " + ].join(' ').trim() : "", + params["metagenome"] ? + "
  • Gourlé H, Karlsson-Lindsjö O, Hayer J and Bongcam+Rudloff E, Simulating Illumina data with InSilicoSeq. Bioinformatics (2018) doi:10.1093/bioinformatics/bty630
    • " : "", "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " ].join(' ').trim() @@ -89,12 +104,8 @@ class WorkflowReadsimulator { meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) + meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + meta["tool_bibliography"] = toolBibliographyText(params) def methods_text = mqc_methods_yaml.text diff --git a/modules/local/capsim.nf b/modules/local/capsim.nf deleted file mode 100644 index 90afbc8..0000000 --- a/modules/local/capsim.nf +++ /dev/null @@ -1,42 +0,0 @@ -process CAPSIM { - - tag "$meta.id" - label 'process_single' - - // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. - //conda "bioconda::crabs=0.1.1-0" - //container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - // 'https://depot.galaxyproject.org/singularity/crabs:0.1.1--pyhb7b1952_0': - // 'docker.io/vmurigneux/japsa' }" - container "docker.io/vmurigneux/japsa" - - input: - tuple val(meta), path(fasta), path(probes), path(index) - - output: - tuple val(meta), path("*.fastq.gz*"), emit: fastq - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def seed = task.ext.seed ?: "${meta.seed}" - def VERSION = '0.9-01a' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. - """ - jsa.sim.capsim \\ - --reference ${fasta} \\ - --probe ${probes} \\ - --ID ${prefix} \\ - --seed ${seed} \\ - ${args2} ${prefix} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - capsim: $VERSION - END_VERSIONS - """ -} diff --git a/modules/local/crabs_dbdownload.nf b/modules/local/crabs_dbdownload.nf index 9796e43..4025182 100644 --- a/modules/local/crabs_dbdownload.nf +++ b/modules/local/crabs_dbdownload.nf @@ -1,14 +1,13 @@ process CRABS_DBDOWNLOAD { tag "" - label 'process_single' + label 'process_medium' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. conda "bioconda::crabs=0.1.1-0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/crabs:0.1.1--pyhb7b1952_0': - 'swordfish/crabs:0.1.4' }" // this container works better than biocontainers/crabs:0.1.1--pyhb7b1952_0 - // no it doesn't, it just gives me a different error message + 'biocontainers/crabs:0.1.1--pyhb7b1952_0' }" input: diff --git a/modules/local/crabs_dbimport.nf b/modules/local/crabs_dbimport.nf index 12992b5..1775e05 100644 --- a/modules/local/crabs_dbimport.nf +++ b/modules/local/crabs_dbimport.nf @@ -1,7 +1,7 @@ process CRABS_DBIMPORT { tag "$meta.id" - label 'process_single' + label 'process_medium' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. conda "bioconda::crabs=0.1.1-0" diff --git a/modules/local/crabs_insilicopcr.nf b/modules/local/crabs_insilicopcr.nf index 62b33f6..1f38b52 100644 --- a/modules/local/crabs_insilicopcr.nf +++ b/modules/local/crabs_insilicopcr.nf @@ -1,7 +1,7 @@ process CRABS_INSILICOPCR { tag "$meta.id" - label 'process_single' + label 'process_medium' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. conda "bioconda::crabs=0.1.1-0" diff --git a/modules/local/create_samplesheet.nf b/modules/local/create_samplesheet.nf new file mode 100644 index 0000000..bce99f3 --- /dev/null +++ b/modules/local/create_samplesheet.nf @@ -0,0 +1,35 @@ +process CREATE_SAMPLESHEET { + tag "$meta.id" + + executor 'local' + memory 100.MB + + input: + tuple val(meta), path(fastq) + + output: + tuple val(meta), path("*.csv"), emit: samplesheet + + exec: + def fastq_1 = "${params.outdir}/${meta.outdir}/${fastq}" + def fastq_2 = '' + if (fastq instanceof List && fastq.size() == 2) { + fastq_1 = "${params.outdir}/${meta.outdir}/${fastq[0]}" + fastq_2 = "${params.outdir}/${meta.outdir}/${fastq[1]}" + } + + // Add relevant fields to the beginning of the map + pipeline_map = [ + sample : "${meta.id}", + fastq_1 : fastq_1, + fastq_2 : fastq_2 + ] + + // Create a samplesheet + samplesheet = pipeline_map.keySet().collect{ '"' + it + '"'}.join(",") + '\n' + samplesheet += pipeline_map.values().collect{ '"' + it + '"'}.join(",") + + // Write samplesheet to file + def samplesheet_file = task.workDir.resolve("${meta.datatype}_${meta.id}.samplesheet.csv") + samplesheet_file.text = samplesheet +} diff --git a/modules/local/insilicoseq_generate.nf b/modules/local/insilicoseq_generate.nf new file mode 100644 index 0000000..14205a7 --- /dev/null +++ b/modules/local/insilicoseq_generate.nf @@ -0,0 +1,64 @@ +process INSILICOSEQ_GENERATE { + + tag "$meta.id" + label 'process_single' + + conda "bioconda::insilicoseq=1.6.0" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/insilicoseq:1.6.0--pyh7cba7a3_0': + 'biocontainers/insilicoseq:1.6.0--pyh7cba7a3_0' }" + + input: + path(fasta) + val(meta) + + output: + tuple val(meta), path("*.fastq.gz*"), emit: fastq + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def seed = task.ext.seed ?: "${meta.seed}" + if (fasta) { + """ + seed=\$(echo $seed | sed 's/\\[//g' | sed 's/\\]//g') + prefix=\$(echo $prefix | sed 's/\\[//g' | sed 's/\\]//g') + + iss generate \\ + --genomes $fasta \\ + --seed \$seed \\ + --output \$prefix \\ + --compress \\ + --cpus $task.cpus \\ + $args + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + insilicoseq: \$(iss --version | sed 's/iss version //g') + END_VERSIONS + """ + } else { + """ + seed=\$(echo $seed | sed 's/\\[//g' | sed 's/\\]//g') + prefix=\$(echo $prefix | sed 's/\\[//g' | sed 's/\\]//g') + + iss generate \\ + --ncbi $args2 \\ + --seed \$seed \\ + --output \$prefix \\ + --compress \\ + --cpus $task.cpus \\ + $args + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + insilicoseq: \$(iss --version | sed 's/iss version //g') + END_VERSIONS + """ + } +} diff --git a/modules/local/japsa_capsim.nf b/modules/local/japsa_capsim.nf new file mode 100644 index 0000000..72a49c6 --- /dev/null +++ b/modules/local/japsa_capsim.nf @@ -0,0 +1,40 @@ +process JAPSA_CAPSIM { + + tag "$meta.id" + label 'process_single' + + container "docker.io/vmurigneux/japsa@sha256:ba74e9c844d115f390be62cde1272cfa5e10492512674ea342a4eeec47840f98" + + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + exit 1, "CAPSIM does not support Conda. Please use Docker / Singularity / Podman instead." + } + + input: + tuple val(meta), path(fasta), path(probes), path(index) + + output: + tuple val(meta), path("*.fastq.gz*"), emit: fastq + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def seed = task.ext.seed ?: "${meta.seed}" + """ + jsa.sim.capsim \\ + --reference ${fasta} \\ + --probe ${probes} \\ + --ID ${prefix} \\ + --seed ${seed} \\ + ${args} ${prefix} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + capsim: \$(jsa | tail -n +2 | head -n 1 | awk -F, '{print \$1}') | sed 's/Version //g' + END_VERSIONS + """ +} diff --git a/modules/local/merge_samplesheets.nf b/modules/local/merge_samplesheets.nf new file mode 100644 index 0000000..1bd3d98 --- /dev/null +++ b/modules/local/merge_samplesheets.nf @@ -0,0 +1,30 @@ +process MERGE_SAMPLESHEETS { + + tag "$meta.id" + label 'process_low' + + conda "conda-forge::sed=4.7" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'nf-core/ubuntu:20.04' }" + + input: + tuple val(meta), path(samplesheet) + + output: + tuple val(meta), path("*.csv"), emit: samplesheet + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + echo \\"sample\\",\\"fastq_1\\",\\"fastq_2\\" > "${prefix}_samplesheet.csv" + for curr_sheet in $samplesheet; do + tail -n +2 "\$curr_sheet" >> "${prefix}_samplesheet.csv" + echo >> "${prefix}_samplesheet.csv" + done + """ +} diff --git a/modules/local/unzip.nf b/modules/local/unzip.nf new file mode 100644 index 0000000..e69fe9f --- /dev/null +++ b/modules/local/unzip.nf @@ -0,0 +1,31 @@ +process UNZIP { + + tag "$file" + label 'process_single' + + conda "conda-forge::unzip=6.0" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/unzip:6.0': + 'biocontainers/unzip:6.0' }" + + input: + path(file) + + output: + path "unziped/*" , emit: file + path "versions.yml", emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + """ + mkdir unziped + unzip ${file} -d unziped + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + unzip: \$(unzip -v | head -n 1 | sed 's/UnZip //g' | cut -d ' ' -f1) + END_VERSIONS + """ +} diff --git a/nextflow.config b/nextflow.config index f2636ac..8badbf4 100644 --- a/nextflow.config +++ b/nextflow.config @@ -13,14 +13,15 @@ params { input = null // References genome = null - //igenomes_base = 's3://ngi-igenomes/igenomes' - igenomes_base = null + igenomes_base = 's3://ngi-igenomes/igenomes' + //igenomes_base = null igenomes_ignore = false fasta = null // Simulation options amplicon = null target_capture = null + metagenome = null // Amplicon options fw_primer = 'GTCGGTAAAACTCGTGCCAGC' @@ -39,6 +40,18 @@ params { probe_fasta = null probe_ref_name = 'Tetrapods-UCE-5Kv1' + // Metagenome options + metagenome_ncbi_db = 'viruses' + metagenome_n_genomes = '10' + metagenome_abundance = 'lognormal' + metagenome_abundance_file = null + metagenome_coverage = null + metagenome_coverage_file = null + metagenome_n_reads = '1M' + metagenome_mode = 'kde' + metagenome_model = 'MiSeq' + metagenome_gc_bias = null + // MultiQC options multiqc_config = null multiqc_title = null @@ -79,7 +92,11 @@ params { validationSchemaIgnoreParams = 'genomes' validationShowHiddenParams = false validate_params = true +} +// Set igenomes base to null to avoid unnecessary permission error +if (params.fasta || !params.target_capture) { + params.igenomes_base = null } // Load base.config by default for all pipelines diff --git a/nextflow_schema.json b/nextflow_schema.json index cc38d36..ad8084d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -58,6 +58,11 @@ "type": "boolean", "description": "Option to simulate UCE target capture data.", "fa_icon": "fas fa-question-circle" + }, + "metagenome": { + "type": "boolean", + "description": "Option to simulate metagenomic data.", + "fa_icon": "fas fa-question-circle" } } }, @@ -80,8 +85,13 @@ "amplicon_ref_source": { "type": "string", "description": "Source for downloading reference database when using `--amplicon` parameter, but not using `--fasta` parameter.", - "help_text": "Can be 'ncbi', 'embl', 'bold', or 'mitofish'. Currently only ncbi and bold works.", - "default": "bold" + "help_text": "Can be 'ncbi', 'embl', or 'bold'.", + "default": "bold", + "enum": [ + "ncbi", + "embl", + "bold" + ] }, "amplicon_ncbi_db": { "type": "string", @@ -162,6 +172,99 @@ } } }, + "metagenome_options": { + "title": "Metagenome options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Options for simulating metagenomic data.", + "properties": { + "metagenome_ncbi_db": { + "type": "string", + "default": "viruses", + "description": "NCBI databases supported by InSilicoSeq. This option gets used when not using the `--fasta` or `--genome` parameters.", + "help_text": "Supported databases are 'bacteria', 'viruses', and 'archaea'. Multiple databases can be used by providing a space seperated string." + }, + "metagenome_n_genomes": { + "type": "string", + "default": "10", + "description": "Number of genomes to download form NCBI. This option gets used when not using the `--fasta` or `--genome` parameters.", + "help_text": "Use multiple values (space seperated) if using multiple NCBI databases" + }, + "metagenome_abundance": { + "type": "string", + "default": "lognormal", + "description": "Abundance distribution.", + "help_text": "Can be 'uniform', 'halfnormal', 'exponential', 'lognormal', or 'zero_inflated_lognormal'", + "enum": [ + "uniform", + "halfnormal", + "exponential", + "lognormal", + "zero_inflated_lognormal" + ] + }, + "metagenome_abundance_file": { + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.tsv$", + "mimetype": "text/plain", + "description": "Path to tab-separated file containing abundance distribution.", + "help_text": "The first column should contain the genome and the second column should contain abundance proportion. It's recommended that the total abundace in your file equals 1." + }, + "metagenome_coverage": { + "type": "string", + "description": "Coverage distribution.", + "help_text": "Can be 'uniform', 'halfnormal', 'exponential', 'lognormal', or 'zero_inflated_lognormal'", + "enum": [ + "uniform", + "halfnormal", + "exponential", + "lognormal", + "zero_inflated_lognormal" + ] + }, + "metagenome_coverage_file": { + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.tsv$", + "mimetype": "text/plain", + "description": "Path to tab-separated file containing coverage information.", + "help_text": "The first column should contain the genome and the second column should contain the coverage (e.g., use the value 20 for a coverage of 20X)." + }, + "metagenome_n_reads": { + "type": "string", + "default": "1M", + "description": "Number of reads to generate.", + "help_text": "Supported suffixes are 'k', 'K', 'm', 'M', 'g', and 'G'." + }, + "metagenome_mode": { + "type": "string", + "default": "kde", + "description": "Can be 'kde', or 'basic'.", + "help_text": "Set this to basic if you don't want to use a model with `--metagenome_model`.", + "enum": [ + "kde", + "basic" + ] + }, + "metagenome_model": { + "type": "string", + "default": "MiSeq", + "description": "Can be 'HiSeq', 'NovaSeq', or 'MiSeq'.", + "enum": [ + "HiSeq", + "NovaSeq", + "MiSeq" + ] + }, + "metagenome_gc_bias": { + "type": "boolean", + "description": "Use this option to prevent simulating reads that have abnormal GC content." + } + } + }, "reference_genome_options": { "title": "Reference genome options", "type": "object", diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index 75c3353..76dbb81 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -16,6 +16,10 @@ workflow AMPLICON_WORKFLOW { main: ch_ref_fasta = Channel.empty() + + // + // MODULE: Run Crabs db_download if user doesn't have a reference database + // if ( !params.fasta ) { CRABS_DBDOWNLOAD () ch_versions = ch_versions.mix(CRABS_DBDOWNLOAD.out.versions) @@ -27,6 +31,9 @@ workflow AMPLICON_WORKFLOW { return [ meta, fasta ] } + // + // MODULE: Run Crabs db_import if user does have a reference database + // } else { ch_meta_fasta = Channel.fromPath(params.fasta) .map { @@ -36,9 +43,6 @@ workflow AMPLICON_WORKFLOW { return [ meta, fasta ] } - // - // MODULE: Run Crabs db_import - // CRABS_DBIMPORT ( ch_meta_fasta ) @@ -72,7 +76,15 @@ workflow AMPLICON_WORKFLOW { ) ch_versions = ch_versions.mix(ART_ILLUMINA.out.versions.first()) + ch_illumina_reads = ART_ILLUMINA.out.fastq + .map { + meta, fastqs -> + meta.outdir = "art_illumina" + meta.datatype = "amplicon_illumina" + return [ meta, fastqs ] + } + emit: - reads = ART_ILLUMINA.out.fastq // channel: [ meta, fastq ] - versions = ch_versions // channel: [ versions.yml ] + reads = ch_illumina_reads // channel: [ meta, fastq ] + versions = ch_versions // channel: [ versions.yml ] } diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index 6250e8b..ed1a1bb 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -2,12 +2,12 @@ // Simulate UCE target capture reads // -include { BOWTIE2_BUILD } from '../../modules/nf-core/bowtie2/build/main' -include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/main' -include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' -include { CAPSIM as CAPSIM_ILLUMINA } from '../../modules/local/capsim' -include { CAPSIM as CAPSIM_PACBIO } from '../../modules/local/capsim' -include { UNZIP } from '../../modules/local/unzip' +include { BOWTIE2_BUILD } from '../../modules/nf-core/bowtie2/build/main' +include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' +include { JAPSA_CAPSIM as CAPSIM_ILLUMINA } from '../../modules/local/japsa_capsim' +include { JAPSA_CAPSIM as CAPSIM_PACBIO } from '../../modules/local/japsa_capsim' +include { UNZIP } from '../../modules/local/unzip' workflow TARGET_CAPTURE_WORKFLOW { take: @@ -18,7 +18,6 @@ workflow TARGET_CAPTURE_WORKFLOW { main: ch_versions = Channel.empty() - // Add a meta map to fasta channel for compatibility with modules ch_meta_fasta = ch_fasta .map { fasta -> @@ -35,6 +34,9 @@ workflow TARGET_CAPTURE_WORKFLOW { ) ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions.first()) + // + // MODULE: Unzip probes file if user is downloading them + // if ( !params.probe_fasta ) { ch_zip_file = Channel.fromPath(params.probe_ref_db[params.probe_ref_name]["url"]) ch_probes = UNZIP ( @@ -88,6 +90,12 @@ workflow TARGET_CAPTURE_WORKFLOW { ) ch_versions = ch_versions.mix(CAPSIM_ILLUMINA.out.versions.first()) ch_illumina_reads = CAPSIM_ILLUMINA.out.fastq + .map { + meta, fastqs -> + meta.outdir = "capsim_illumina" + meta.datatype = "target_capture_illumina" + return [ meta, fastqs ] + } // // MODULE: Simulate target capture pacbio reads @@ -99,6 +107,12 @@ workflow TARGET_CAPTURE_WORKFLOW { ) ch_versions = ch_versions.mix(CAPSIM_PACBIO.out.versions.first()) ch_pacbio_reads = CAPSIM_PACBIO.out.fastq + .map { + meta, fastqs -> + meta.outdir = "capsim_pacbio" + meta.datatype = "target_capture_pacbio" + return [ meta, fastqs ] + } } emit: diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 50a9168..911dafc 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -32,6 +32,13 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ +// +// MODULE: Local modules +// +include { INSILICOSEQ_GENERATE } from '../modules/local/insilicoseq_generate' +include { CREATE_SAMPLESHEET } from '../modules/local/create_samplesheet' +include { MERGE_SAMPLESHEETS } from '../modules/local/merge_samplesheets' + // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // @@ -62,9 +69,9 @@ def multiqc_report = [] workflow READSIMULATOR { - ch_versions = Channel.empty() - ch_input = Channel.fromSamplesheet("input") - ch_fastqc_input = Channel.empty() + ch_versions = Channel.empty() + ch_input = Channel.fromSamplesheet("input") + ch_simulated_reads = Channel.empty() if ( params.fasta ) { ch_fasta = Channel.fromPath(params.fasta) @@ -86,12 +93,12 @@ workflow READSIMULATOR { ch_fasta.ifEmpty([]), ch_input ) - ch_versions = ch_versions.mix(AMPLICON_WORKFLOW.out.versions.first()) - ch_fastqc_input = ch_fastqc_input.mix(AMPLICON_WORKFLOW.out.reads) + ch_versions = ch_versions.mix(AMPLICON_WORKFLOW.out.versions.first()) + ch_simulated_reads = ch_simulated_reads.mix(AMPLICON_WORKFLOW.out.reads) } // - // SUBWORKFLOW: Simulate UCE taget capture reads + // SUBWORKFLOW: Simulate UCE target capture reads // if ( params.target_capture ) { TARGET_CAPTURE_WORKFLOW ( @@ -99,19 +106,59 @@ workflow READSIMULATOR { ch_input, ch_probes.ifEmpty([]) ) - ch_versions = ch_versions.mix(TARGET_CAPTURE_WORKFLOW.out.versions.first()) - ch_fastqc_input = ch_fastqc_input.mix(TARGET_CAPTURE_WORKFLOW.out.illumina_reads) - ch_fastqc_input = ch_fastqc_input.mix(TARGET_CAPTURE_WORKFLOW.out.pacbio_reads) + ch_versions = ch_versions.mix(TARGET_CAPTURE_WORKFLOW.out.versions.first()) + ch_simulated_reads = ch_simulated_reads.mix(TARGET_CAPTURE_WORKFLOW.out.illumina_reads) + ch_simulated_reads = ch_simulated_reads.mix(TARGET_CAPTURE_WORKFLOW.out.pacbio_reads) + } + + // + // MODULE: Simulate metagenomic reads + // + if ( params.metagenome ) { + INSILICOSEQ_GENERATE ( + ch_fasta.ifEmpty([]).first(), + ch_input + ) + ch_versions = ch_versions.mix(INSILICOSEQ_GENERATE.out.versions.first()) + ch_metagenome_reads = INSILICOSEQ_GENERATE.out.fastq + .map { + meta, fastqs -> + meta.outdir = "insilicoseq" + meta.datatype = "metagenomic_illumina" + return [ meta, fastqs ] + } + ch_simulated_reads = ch_simulated_reads.mix(ch_metagenome_reads) } - // MODULE: Create sample sheet - // I plan on having a module here that creates a samplesheet that can be used with other nf-core pipelines + // MODULE: Create sample sheet (just the header and one row) + CREATE_SAMPLESHEET ( + ch_simulated_reads + ) + + // Group the samplesheets by datatype so that we can merge them + ch_samplesheets = CREATE_SAMPLESHEET.out.samplesheet + .map { + meta, samplesheet -> + tuple( meta.datatype, meta, samplesheet ) + } + .groupTuple() + .map { + datatype, old_meta, samplesheet -> + def meta = [:] + meta.id = datatype + return [ meta, samplesheet ] + } + + // MODULE: Merge the samplesheets by data type + MERGE_SAMPLESHEETS ( + ch_samplesheets + ) // // MODULE: Run FastQC // FASTQC ( - ch_fastqc_input + ch_simulated_reads ) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) From 085444fc8139cb26745f4dbbc2da0028954bd4ee Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Mon, 9 Oct 2023 23:52:32 +0000 Subject: [PATCH 07/89] Improved documentation --- README.md | 2 +- lib/WorkflowReadsimulator.groovy | 1 - nextflow.config | 1 - nextflow_schema.json | 17 +++++++++++++++++ 4 files changed, 18 insertions(+), 3 deletions(-) diff --git a/README.md b/README.md index 593350f..9805c57 100644 --- a/README.md +++ b/README.md @@ -68,7 +68,7 @@ For more details about the output files and reports, please refer to the ## Credits -nf-core/readsimulator was originally written by Adam Bennett ([@a4000](https://github.com/a4000)) for use at the [MinderooFoundation](https://www.minderoo.org/). +nf-core/readsimulator was originally written by [Adam Bennett](https://github.com/a4000) for use at the [MinderooFoundation](https://www.minderoo.org/). We thank the following people for their extensive assistance in the development of this pipeline (in alphabetical order): diff --git a/lib/WorkflowReadsimulator.groovy b/lib/WorkflowReadsimulator.groovy index b27d813..cfe8b1b 100755 --- a/lib/WorkflowReadsimulator.groovy +++ b/lib/WorkflowReadsimulator.groovy @@ -17,7 +17,6 @@ class WorkflowReadsimulator { if (!params.fasta && params.target_capture) { Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." } - } // diff --git a/nextflow.config b/nextflow.config index 8badbf4..49c72bd 100644 --- a/nextflow.config +++ b/nextflow.config @@ -69,7 +69,6 @@ params { hook_url = null help = false version = false - schema_ignore_params = 'probe_ref_db' // Config options config_profile_name = null diff --git a/nextflow_schema.json b/nextflow_schema.json index ad8084d..ac19198 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -169,6 +169,11 @@ "Hymenoptera-2.5Kv2", "Anthozoa-1.7Kv1" ] + }, + "probe_ref_db": { + "type": "string", + "description": "This parameter can be ignored if you want to download probes from https://www.ultraconserved.org/", + "help_text": "If you want to download the probes from an alternative source, this can be achieved by using `--probe_ref_db \"'$probe_ref_name' {url='$url'}\"`" } } }, @@ -510,6 +515,18 @@ { "$ref": "#/definitions/input_output_options" }, + { + "$ref": "#/definitions/simulation_options" + }, + { + "$ref": "#/definitions/amplicon_options" + }, + { + "$ref": "#/definitions/target_capture_options" + }, + { + "$ref": "#/definitions/metagenome_options" + }, { "$ref": "#/definitions/reference_genome_options" }, From ae82218c750160964012388efc9ea1519d97e326 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 12 Oct 2023 04:33:46 +0000 Subject: [PATCH 08/89] fixed bug with meta map after insilicoseq generate --- conf/test.config | 4 ++-- modules/local/create_samplesheet.nf | 2 +- subworkflows/local/amplicon_workflow.nf | 8 ++++---- subworkflows/local/target_capture_workflow.nf | 8 ++++---- workflows/readsimulator.nf | 8 ++++---- 5 files changed, 15 insertions(+), 15 deletions(-) diff --git a/conf/test.config b/conf/test.config index d7b277f..947cb4d 100644 --- a/conf/test.config +++ b/conf/test.config @@ -23,7 +23,7 @@ params { // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + //fasta = '' - // Genome references - genome = 'R64-1-1' + metagenome = true } diff --git a/modules/local/create_samplesheet.nf b/modules/local/create_samplesheet.nf index bce99f3..c48c0f3 100644 --- a/modules/local/create_samplesheet.nf +++ b/modules/local/create_samplesheet.nf @@ -30,6 +30,6 @@ process CREATE_SAMPLESHEET { samplesheet += pipeline_map.values().collect{ '"' + it + '"'}.join(",") // Write samplesheet to file - def samplesheet_file = task.workDir.resolve("${meta.datatype}_${meta.id}.samplesheet.csv") + def samplesheet_file = task.workDir.resolve("${meta.datatype}_${meta.id}.samplesheet.csv") samplesheet_file.text = samplesheet } diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index 76dbb81..40694ab 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -27,7 +27,7 @@ workflow AMPLICON_WORKFLOW { .map { fasta -> def meta = [:] - meta.id = "amplicon" + meta.id = "amplicon" return [ meta, fasta ] } @@ -39,14 +39,14 @@ workflow AMPLICON_WORKFLOW { .map { fasta -> def meta = [:] - meta.id = "amplicon" + meta.id = "amplicon" return [ meta, fasta ] } CRABS_DBIMPORT ( ch_meta_fasta ) - ch_versions = ch_versions.mix(CRABS_DBIMPORT.out.versions) + ch_versions = ch_versions.mix(CRABS_DBIMPORT.out.versions) ch_ref_fasta = CRABS_DBIMPORT.out.fasta } @@ -79,7 +79,7 @@ workflow AMPLICON_WORKFLOW { ch_illumina_reads = ART_ILLUMINA.out.fastq .map { meta, fastqs -> - meta.outdir = "art_illumina" + meta.outdir = "art_illumina" meta.datatype = "amplicon_illumina" return [ meta, fastqs ] } diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index ed1a1bb..f3c8a42 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -22,7 +22,7 @@ workflow TARGET_CAPTURE_WORKFLOW { .map { fasta -> def meta = [:] - meta.id = "target_capture" + meta.id = "target_capture" return [ meta, fasta ] } @@ -88,11 +88,11 @@ workflow TARGET_CAPTURE_WORKFLOW { CAPSIM_ILLUMINA ( ch_capsim_input ) - ch_versions = ch_versions.mix(CAPSIM_ILLUMINA.out.versions.first()) + ch_versions = ch_versions.mix(CAPSIM_ILLUMINA.out.versions.first()) ch_illumina_reads = CAPSIM_ILLUMINA.out.fastq .map { meta, fastqs -> - meta.outdir = "capsim_illumina" + meta.outdir = "capsim_illumina" meta.datatype = "target_capture_illumina" return [ meta, fastqs ] } @@ -105,7 +105,7 @@ workflow TARGET_CAPTURE_WORKFLOW { CAPSIM_PACBIO ( ch_capsim_input ) - ch_versions = ch_versions.mix(CAPSIM_PACBIO.out.versions.first()) + ch_versions = ch_versions.mix(CAPSIM_PACBIO.out.versions.first()) ch_pacbio_reads = CAPSIM_PACBIO.out.fastq .map { meta, fastqs -> diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 911dafc..b2d2eff 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -106,7 +106,7 @@ workflow READSIMULATOR { ch_input, ch_probes.ifEmpty([]) ) - ch_versions = ch_versions.mix(TARGET_CAPTURE_WORKFLOW.out.versions.first()) + ch_versions = ch_versions.mix(TARGET_CAPTURE_WORKFLOW.out.versions.first()) ch_simulated_reads = ch_simulated_reads.mix(TARGET_CAPTURE_WORKFLOW.out.illumina_reads) ch_simulated_reads = ch_simulated_reads.mix(TARGET_CAPTURE_WORKFLOW.out.pacbio_reads) } @@ -123,8 +123,8 @@ workflow READSIMULATOR { ch_metagenome_reads = INSILICOSEQ_GENERATE.out.fastq .map { meta, fastqs -> - meta.outdir = "insilicoseq" - meta.datatype = "metagenomic_illumina" + meta[0].outdir = "insilicoseq" + meta[0].datatype = "metagenomic_illumina" return [ meta, fastqs ] } ch_simulated_reads = ch_simulated_reads.mix(ch_metagenome_reads) @@ -145,7 +145,7 @@ workflow READSIMULATOR { .map { datatype, old_meta, samplesheet -> def meta = [:] - meta.id = datatype + meta.id = datatype return [ meta, samplesheet ] } From d2368e1eb8182a632698fc2a2110e707d3a68aa9 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 25 Oct 2023 02:31:11 +0000 Subject: [PATCH 09/89] Added test profiles --- README.md | 2 +- conf/test.config | 9 ++++----- conf/test_full.config | 11 ++++++----- nextflow.config | 3 +-- nextflow_schema.json | 5 ----- workflows/readsimulator.nf | 2 +- 6 files changed, 13 insertions(+), 19 deletions(-) diff --git a/README.md b/README.md index 9805c57..9c0718a 100644 --- a/README.md +++ b/README.md @@ -68,7 +68,7 @@ For more details about the output files and reports, please refer to the ## Credits -nf-core/readsimulator was originally written by [Adam Bennett](https://github.com/a4000) for use at the [MinderooFoundation](https://www.minderoo.org/). +nf-core/readsimulator was originally written by [Adam Bennett](https://github.com/a4000) for use at the [Minderoo Foundation's OceanOmics project](https://www.minderoo.org/oceanomics). We thank the following people for their extensive assistance in the development of this pipeline (in alphabetical order): diff --git a/conf/test.config b/conf/test.config index 947cb4d..0793c09 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,10 +20,9 @@ params { max_time = '6.h' // Input data - // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' - //fasta = '' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/samplesheet/test_samplesheet.csv' + fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/testdata/GCF_024334085.1_ASM2433408v1_genomic.fna.gz' - metagenome = true + target_capture = true + probe_ref_name = 'Diptera-2.7Kv1' } diff --git a/conf/test_full.config b/conf/test_full.config index 730c542..5bc8140 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -15,10 +15,11 @@ params { config_profile_description = 'Full test dataset to check pipeline function' // Input data for full size test - // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/samplesheet/test_samplesheet.csv' + fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/Sarek/testdata/hg38.chrM.fa' - // Genome references - genome = 'R64-1-1' + metagenome = true + metagenome_n_genomes = '1' + metagenome_n_reads = '10' + amplicon = true } diff --git a/nextflow.config b/nextflow.config index 49c72bd..c3fcc49 100644 --- a/nextflow.config +++ b/nextflow.config @@ -14,7 +14,6 @@ params { // References genome = null igenomes_base = 's3://ngi-igenomes/igenomes' - //igenomes_base = null igenomes_ignore = false fasta = null @@ -88,7 +87,7 @@ params { // Schema validation default options validationFailUnrecognisedParams = false validationLenientMode = false - validationSchemaIgnoreParams = 'genomes' + validationSchemaIgnoreParams = 'genomes,probe_ref_db' validationShowHiddenParams = false validate_params = true } diff --git a/nextflow_schema.json b/nextflow_schema.json index ac19198..5853bab 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -169,11 +169,6 @@ "Hymenoptera-2.5Kv2", "Anthozoa-1.7Kv1" ] - }, - "probe_ref_db": { - "type": "string", - "description": "This parameter can be ignored if you want to download probes from https://www.ultraconserved.org/", - "help_text": "If you want to download the probes from an alternative source, this can be achieved by using `--probe_ref_db \"'$probe_ref_name' {url='$url'}\"`" } } }, diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index b2d2eff..5615630 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -125,7 +125,7 @@ workflow READSIMULATOR { meta, fastqs -> meta[0].outdir = "insilicoseq" meta[0].datatype = "metagenomic_illumina" - return [ meta, fastqs ] + return [ meta[0], fastqs ] } ch_simulated_reads = ch_simulated_reads.mix(ch_metagenome_reads) } From 73f6b88d623eb540bc1428485c67a5b39eb9c1d2 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 25 Oct 2023 02:59:16 +0000 Subject: [PATCH 10/89] removed dev from version --- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 28d3fd1..4ff4b58 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/readsimulator + This report has been generated by the nf-core/readsimulator analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-readsimulator-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index c3fcc49..213331e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -268,7 +268,7 @@ manifest { description = """A workflow to simulate reads""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.0dev' + version = '1.0' doi = '' } From 4b437bef1d2370279720a607e26f8dcb0bb20bd6 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 25 Oct 2023 03:38:00 +0000 Subject: [PATCH 11/89] made changes based on linting comment --- conf/ref_databases.config | 4 ++-- modules/local/japsa_capsim.nf | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/conf/ref_databases.config b/conf/ref_databases.config index f7d368d..9d38a64 100644 --- a/conf/ref_databases.config +++ b/conf/ref_databases.config @@ -7,7 +7,7 @@ * Each entry requires as a minimum: title, url, citation */ - params { +params { probe_ref_db { 'Tetrapods-UCE-2.5Kv1' { title = 'Tetrapods; 2,560 baits for 2,386 UCEs; version 1' @@ -65,4 +65,4 @@ citation = 'Quattrini. A. M., Faircloth. B. C., Duenas. L. F., Bridge. T. C. L., Brugler. M. R., Calixto-Botia. I. F., DeLeo. D. M., Foret. S., Herrera. S., Lee. S. M. Y., Miller. D. J., Prada. C., Radis-Baptista. G., Ramirez-Portilla. C., Sanchez. J. A., Rodriguez. E., & McFadden. C. S. (2017). Universal target-enrichment baits for anthozoan (Cnidaria) phylogenomics: New approaches to long-standing problems. Molecular Ecology Resources. 18(2), 281-295. https://doi.org/10.1111/1755-0998.12736' } } - } +} diff --git a/modules/local/japsa_capsim.nf b/modules/local/japsa_capsim.nf index 72a49c6..00a7bbb 100644 --- a/modules/local/japsa_capsim.nf +++ b/modules/local/japsa_capsim.nf @@ -5,7 +5,7 @@ process JAPSA_CAPSIM { container "docker.io/vmurigneux/japsa@sha256:ba74e9c844d115f390be62cde1272cfa5e10492512674ea342a4eeec47840f98" - // Exit if running this module with -profile conda / -profile mamba + // Exit if running this module with -profile conda / -profile mamba if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { exit 1, "CAPSIM does not support Conda. Please use Docker / Singularity / Podman instead." } From 53ec3cf725b4da7dcf5ac6f4e861e579d600e351 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 25 Oct 2023 04:42:38 +0000 Subject: [PATCH 12/89] updated files to match template form nf-core tools 2.10 --- .github/CONTRIBUTING.md | 4 +- .github/workflows/linting.yml | 2 +- CHANGELOG.md | 11 ++- CODE_OF_CONDUCT.md | 133 ++++++++++++++++++++------ assets/multiqc_config.yml | 4 +- docs/output.md | 22 ++--- lib/NfcoreTemplate.groovy | 16 ++++ modules/nf-core/art/illumina/meta.yml | 5 +- 8 files changed, 142 insertions(+), 55 deletions(-) diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 6d9461b..da7566d 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,7 +9,9 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -> If you need help using or modifying nf-core/readsimulator then the best place to ask is on the nf-core Slack [#readsimulator](https://nfcore.slack.com/channels/readsimulator) channel ([join our Slack here](https://nf-co.re/join/slack)). +:::info +If you need help using or modifying nf-core/readsimulator then the best place to ask is on the nf-core Slack [#readsimulator](https://nfcore.slack.com/channels/readsimulator) channel ([join our Slack here](https://nf-co.re/join/slack)). +::: ## Contribution workflow diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 888cb4b..b8bdd21 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -78,7 +78,7 @@ jobs: - uses: actions/setup-python@v4 with: - python-version: "3.8" + python-version: "3.11" architecture: "x64" - name: Install dependencies diff --git a/CHANGELOG.md b/CHANGELOG.md index df450a9..85346ed 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,15 +3,16 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.0dev - [date] +## v1.0 - [date] Initial release of nf-core/readsimulator, created with the [nf-core](https://nf-co.re/) template. ### `Added` -Option to simulate amplicon sequencing reads -Option to simulate metagenomic sequencing reads -Option to simulate target capture sequencing reads -Samplesheet creation after reads have been simulated + +- Option to simulate amplicon sequencing reads +- Option to simulate metagenomic sequencing reads +- Option to simulate target capture sequencing reads +- Samplesheet creation after reads have been simulated ### `Fixed` diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index f4fd052..c089ec7 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -1,18 +1,20 @@ -# Code of Conduct at nf-core (v1.0) +# Code of Conduct at nf-core (v1.4) ## Our Pledge -In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: - Age +- Ability - Body size +- Caste - Familial status - Gender identity and expression - Geographical location - Level of experience - Nationality and national origins - Native language -- Physical and neurological ability +- Neurodiversity - Race or ethnicity - Religion - Sexual identity and orientation @@ -22,80 +24,133 @@ Please note that the list above is alphabetised and is therefore not ranked in a ## Preamble -> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. +:::note +This Code of Conduct (CoC) has been drafted by Renuka Kudva, Cris Tuñí, and Michael Heuer, with input from the nf-core Core Team and Susanna Marquez from the nf-core community. "We", in this document, refers to the Safety Officers and members of the nf-core Core Team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will be amended periodically to keep it up-to-date. In case of any dispute, the most current version will apply. +::: -An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). + +Our Safety Officers are Saba Nafees, Cris Tuñí, and Michael Heuer. nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. -We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. +We have therefore adopted this CoC, which we require all members of our community and attendees of nf-core events to adhere to in all our workspaces at all times. Workspaces include, but are not limited to, Slack, meetings on Zoom, gather.town, YouTube live etc. -Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. +Our CoC will be strictly enforced and the nf-core team reserves the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. -We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. +We ask all members of our community to help maintain supportive and productive workspaces and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. -Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re +Questions, concerns, or ideas on what we can include? Contact members of the Safety Team on Slack or email safety [at] nf-co [dot] re. ## Our Responsibilities -The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. +Members of the Safety Team (the Safety Officers) are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. -The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. +The Safety Team, in consultation with the nf-core core team, have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this CoC, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. -Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. +Members of the core team or the Safety Team who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and will be subject to the same actions as others in violation of the CoC. -## When are where does this Code of Conduct apply? +## When and where does this Code of Conduct apply? -Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events, such as hackathons, workshops, bytesize, and collaborative workspaces on gather.town. These guidelines include, but are not limited to, the following (listed alphabetically and therefore in no order of preference): - Communicating with an official project email address. - Communicating with community members within the nf-core Slack channel. - Participating in hackathons organised by nf-core (both online and in-person events). -- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. -- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence, and on the nf-core gather.town workspace. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, gather.town, Jitsi, YouTube live etc. - Representing nf-core on social media. This includes both official and personal accounts. ## nf-core cares 😊 -nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include, but are not limited to, the following (listed in alphabetical order): - Ask for consent before sharing another community member’s personal information (including photographs) on social media. - Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. -- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Celebrate your accomplishments! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) - Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) - Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) - Focus on what is best for the team and the community. (When in doubt, ask) -- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. +- Accept feedback, yet be unafraid to question, deliberate, and learn. - Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) -- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communication to be kind.**) - Take breaks when you feel like you need them. -- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) +- Use welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack) ## nf-core frowns on 😕 -The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this CoC. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces: - Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. - “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. - Spamming or trolling of individuals on social media. -- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. -- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. +- Use of sexual or discriminatory imagery, comments, jokes, or unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion, or work experience. ### Online Trolling -The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the risk of online trolling. This is unacceptable — reports of such behaviour will be taken very seriously and perpetrators will be excluded from activities immediately. -All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. +All community members are **required** to ask members of the group they are working with for explicit consent prior to taking screenshots of individuals during video calls. -## Procedures for Reporting CoC violations +## Procedures for reporting CoC violations If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. -You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). +You can reach out to members of the Safety Team (Saba Nafees, Cris Tuñí, and Michael Heuer) on Slack. Alternatively, contact a member of the nf-core core team [nf-core core team](https://nf-co.re/about), and they will forward your concerns to the Safety Team. + +Issues directly concerning members of the Core Team or the Safety Team will be dealt with by other members of the core team and the safety manager — possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson and details will be shared in due course. + +All reports will be handled with the utmost discretion and confidentiality. + +You can also report any CoC violations to safety [at] nf-co [dot] re. In your email report, please do your best to include: + +- Your contact information. +- Identifying information (e.g. names, nicknames, pseudonyms) of the participant who has violated the Code of Conduct. +- The behaviour that was in violation and the circumstances surrounding the incident. +- The approximate time of the behaviour (if different than the time the report was made). +- Other people involved in the incident, if applicable. +- If you believe the incident is ongoing. +- If there is a publicly available record (e.g. mailing list record, a screenshot). +- Any additional information. + +After you file a report, one or more members of our Safety Team will contact you to follow up on your report. + +## Who will read and handle reports + +All reports will be read and handled by the members of the Safety Team at nf-core. + +If members of the Safety Team are deemed to have a conflict of interest with a report, they will be required to recuse themselves as per our Code of Conduct and will not have access to any follow-ups. + +To keep this first report confidential from any of the Safety Team members, please submit your first report by direct messaging on Slack/direct email to any of the nf-core members you are comfortable disclosing the information to, and be explicit about which member(s) you do not consent to sharing the information with. + +## Reviewing reports + +After receiving the report, members of the Safety Team will review the incident report to determine whether immediate action is required, for example, whether there is immediate threat to participants’ safety. + +The Safety Team, in consultation with members of the nf-core core team, will assess the information to determine whether the report constitutes a Code of Conduct violation, for them to decide on a course of action. + +In the case of insufficient information, one or more members of the Safety Team may contact the reporter, the reportee, or any other attendees to obtain more information. -Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. +Once additional information is gathered, the Safety Team will collectively review and decide on the best course of action to take, if any. The Safety Team reserves the right to not act on a report. -All reports will be handled with utmost discretion and confidentially. +## Confidentiality + +All reports, and any additional information included, are only shared with the team of safety officers (and possibly members of the core team, in case the safety officer is in violation of the CoC). We will respect confidentiality requests for the purpose of protecting victims of abuse. + +We will not name harassment victims, beyond discussions between the safety officer and members of the nf-core team, without the explicit consent of the individuals involved. + +## Enforcement + +Actions taken by the nf-core’s Safety Team may include, but are not limited to: + +- Asking anyone to stop a behaviour. +- Asking anyone to leave the event and online spaces either temporarily, for the remainder of the event, or permanently. +- Removing access to the gather.town and Slack, either temporarily or permanently. +- Communicating to all participants to reinforce our expectations for conduct and remind what is unacceptable behaviour; this may be public for practical reasons. +- Communicating to all participants that an incident has taken place and how we will act or have acted — this may be for the purpose of letting event participants know we are aware of and dealing with the incident. +- Banning anyone from participating in nf-core-managed spaces, future events, and activities, either temporarily or permanently. +- No action. ## Attribution and Acknowledgements @@ -106,6 +161,22 @@ All reports will be handled with utmost discretion and confidentially. ## Changelog -### v1.0 - March 12th, 2021 +### v1.4 - February 8th, 2022 + +- Included a new member of the Safety Team. Corrected a typographical error in the text. + +### v1.3 - December 10th, 2021 + +- Added a statement that the CoC applies to nf-core gather.town workspaces. Corrected typographical errors in the text. + +### v1.2 - November 12th, 2021 + +- Removed information specific to reporting CoC violations at the Hackathon in October 2021. + +### v1.1 - October 14th, 2021 + +- Updated with names of new Safety Officers and specific information for the hackathon in October 2021. + +### v1.0 - March 15th, 2021 - Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 4ff4b58..567d0f9 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/readsimulator + This report has been generated by the nf-core/readsimulator analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-readsimulator-methods-description": order: -1000 diff --git a/docs/output.md b/docs/output.md index b338943..0574a49 100644 --- a/docs/output.md +++ b/docs/output.md @@ -10,17 +10,17 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: - - [ART](#art) - ART Illumina simulated reads - - [Bowtie2](#bowtie2) - Bowtie2 alignments - - [CapSim](#capsim) - CapSim simulated reads (Illumina or Pacbio) - - [CRABS](#crabs) - Formated reference database for simulating amplicon reads - - [FastQC](#fastqc) - Raw read QC - - [InSilicoSeq](#insilicoseq) - InSilicoSeq simulated metagenomic reads - - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution - - [Samplesheet](#samplesheet) - Samplesheets produced during the running of the pipeline - - [SAMtools](#samtools) - SAMtools index - - [Unzip](#unzip) - Unziped probe file +- [ART](#art) - ART Illumina simulated reads +- [Bowtie2](#bowtie2) - Bowtie2 alignments +- [CapSim](#capsim) - CapSim simulated reads (Illumina or Pacbio) +- [CRABS](#crabs) - Formated reference database for simulating amplicon reads +- [FastQC](#fastqc) - Raw read QC +- [InSilicoSeq](#insilicoseq) - InSilicoSeq simulated metagenomic reads +- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline +- [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution +- [Samplesheet](#samplesheet) - Samplesheets produced during the running of the pipeline +- [SAMtools](#samtools) - SAMtools index +- [Unzip](#unzip) - Unziped probe file ### ART diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 408951a..01b8653 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -3,6 +3,7 @@ // import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput class NfcoreTemplate { @@ -222,6 +223,21 @@ class NfcoreTemplate { } } + // + // Dump pipeline parameters in a json file + // + public static void dump_parameters(workflow, params) { + def output_d = new File("${params.outdir}/pipeline_info/") + if (!output_d.exists()) { + output_d.mkdirs() + } + + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def output_pf = new File(output_d, "params_${timestamp}.json") + def jsonStr = JsonOutput.toJson(params) + output_pf.text = JsonOutput.prettyPrint(jsonStr) + } + // // Print pipeline summary on completion // diff --git a/modules/nf-core/art/illumina/meta.yml b/modules/nf-core/art/illumina/meta.yml index dad0bcd..ed9960b 100644 --- a/modules/nf-core/art/illumina/meta.yml +++ b/modules/nf-core/art/illumina/meta.yml @@ -11,7 +11,6 @@ tools: homepage: "https://www.niehs.nih.gov/research/resources/software/biostatistics/art/index.cfm" doi: "10.1093/bioinformatics/btr708" licence: "GPL version 3 license" - input: - meta: type: map @@ -31,7 +30,6 @@ input: - read_length: type: integer description: The length of reads to be simulated - output: - versions: type: file @@ -54,6 +52,5 @@ output: type: file description: OPTIONAL Alignment file in SAM format of the simulated reads. Enabled with -sam/--samout. pattern: "*.sam" - authors: - - "@MarieLataretu" \ No newline at end of file + - "@MarieLataretu" From 6d07cc4e820b1cf5db86cd831d763a8563955013 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 25 Oct 2023 04:53:14 +0000 Subject: [PATCH 13/89] fixed prettier issue --- docs/output.md | 1 - docs/usage.md | 8 ++++---- nextflow_schema.json | 33 +++++---------------------------- 3 files changed, 9 insertions(+), 33 deletions(-) diff --git a/docs/output.md b/docs/output.md index 0574a49..a8763cc 100644 --- a/docs/output.md +++ b/docs/output.md @@ -62,7 +62,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - `*_1.fastq.gz`: Read 1 files simulating Pacbio reads. The prefix will be the sample name found in the samplesheet. - `*_1.fastq.gz`: Read 2 files simulating Pacbio reads. The prefix will be the sample name found in the samplesheet. -
    [CapSim](https://academic.oup.com/bioinformatics/article/34/5/873/4575140) is a tool to simulate capture sequencing reads. It's part of the Japsa package. For further reading and documentation see the [CapSim documentation](https://japsa.readthedocs.io/en/latest/tools/jsa.sim.capsim.html). diff --git a/docs/usage.md b/docs/usage.md index a78b82c..30e9fc6 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -32,10 +32,10 @@ sample_5,5 sample_6,6 ``` -| Column | Description | -| --------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. This entry will be used as the prefix for many output files. Spaces in sample names are automatically converted to underscores (`_`). | -| `seed` | Seed used with tools that have a seed parameter for Random Number Generation. | +| Column | Description | +| -------- | --------------------------------------------------------------------------------------------------------------------------------------------------------- | +| `sample` | Custom sample name. This entry will be used as the prefix for many output files. Spaces in sample names are automatically converted to underscores (`_`). | +| `seed` | Seed used with tools that have a seed parameter for Random Number Generation. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. diff --git a/nextflow_schema.json b/nextflow_schema.json index 5853bab..a524295 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -87,11 +87,7 @@ "description": "Source for downloading reference database when using `--amplicon` parameter, but not using `--fasta` parameter.", "help_text": "Can be 'ncbi', 'embl', or 'bold'.", "default": "bold", - "enum": [ - "ncbi", - "embl", - "bold" - ] + "enum": ["ncbi", "embl", "bold"] }, "amplicon_ncbi_db": { "type": "string", @@ -195,13 +191,7 @@ "default": "lognormal", "description": "Abundance distribution.", "help_text": "Can be 'uniform', 'halfnormal', 'exponential', 'lognormal', or 'zero_inflated_lognormal'", - "enum": [ - "uniform", - "halfnormal", - "exponential", - "lognormal", - "zero_inflated_lognormal" - ] + "enum": ["uniform", "halfnormal", "exponential", "lognormal", "zero_inflated_lognormal"] }, "metagenome_abundance_file": { "type": "string", @@ -216,13 +206,7 @@ "type": "string", "description": "Coverage distribution.", "help_text": "Can be 'uniform', 'halfnormal', 'exponential', 'lognormal', or 'zero_inflated_lognormal'", - "enum": [ - "uniform", - "halfnormal", - "exponential", - "lognormal", - "zero_inflated_lognormal" - ] + "enum": ["uniform", "halfnormal", "exponential", "lognormal", "zero_inflated_lognormal"] }, "metagenome_coverage_file": { "type": "string", @@ -244,20 +228,13 @@ "default": "kde", "description": "Can be 'kde', or 'basic'.", "help_text": "Set this to basic if you don't want to use a model with `--metagenome_model`.", - "enum": [ - "kde", - "basic" - ] + "enum": ["kde", "basic"] }, "metagenome_model": { "type": "string", "default": "MiSeq", "description": "Can be 'HiSeq', 'NovaSeq', or 'MiSeq'.", - "enum": [ - "HiSeq", - "NovaSeq", - "MiSeq" - ] + "enum": ["HiSeq", "NovaSeq", "MiSeq"] }, "metagenome_gc_bias": { "type": "boolean", From 0eae4bc84ecab7e48046f43faab0032f033a7d80 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 26 Oct 2023 06:47:52 +0000 Subject: [PATCH 14/89] fixed small mistakes in README --- README.md | 6 +----- 1 file changed, 1 insertion(+), 5 deletions(-) diff --git a/README.md b/README.md index 9c0718a..402afae 100644 --- a/README.md +++ b/README.md @@ -12,9 +12,7 @@ ## Introduction -**nf-core/readsimulator** is a bioinformatics pipeline that ... - -nf-core/readsimulator is a pipeline to simulate sequencing reads. The pipeline currently supports simulating Amplicon, Metagenomic, and Target Capture data. It takes a samplesheet with sample names and seeds for random generation to produce simulated fastq files and a samplesheet that contains the paths to the fastq files. +**nf-core/readsimulator** is a pipeline to simulate sequencing reads. The pipeline currently supports simulating Amplicon, Metagenomic, and Target Capture data. It takes a samplesheet with sample names and seeds for random generation to produce simulated fastq files and a samplesheet that contains the paths to the fastq files. @@ -41,8 +39,6 @@ sample_1,1 Each row represents an output sample. ---> - Now, you can run the pipeline using: ```bash From c3c53de90a19d5945c71326829f4facda90967a3 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:28:57 +0800 Subject: [PATCH 15/89] Update docs/output.md Co-authored-by: Friederike Hanssen --- docs/output.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/output.md b/docs/output.md index a8763cc..c8bae09 100644 --- a/docs/output.md +++ b/docs/output.md @@ -114,7 +114,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d -[InSilicoSeq](https://academic.oup.com/bioinformatics/article/35/3/521/5055123) is a tool for simulating Illumina metgenomic sequencing reads. For further reading and documentation see the [InSilicoSeq documentation](https://insilicoseq.readthedocs.io/en/latest/). +[InSilicoSeq](https://academic.oup.com/bioinformatics/article/35/3/521/5055123) is a tool for simulating Illumina metagenomic sequencing reads. For further reading and documentation see the [InSilicoSeq documentation](https://insilicoseq.readthedocs.io/en/latest/). ### MultiQC From 9b5d7755c91bbb3a7a213041290da92f89be1e70 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:30:49 +0800 Subject: [PATCH 16/89] Update docs/usage.md Co-authored-by: Friederike Hanssen --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index 30e9fc6..a519d7b 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -6,7 +6,7 @@ ## Introduction -The pipeline curently support simulating amplicon, metagenomic, and target capture sequencing reads. The different modes can be selected with `--amplicon`, `--metagenome`, or `--target_capture` respectively. +The pipeline currently supporst simulating amplicon, metagenomic, and target capture sequencing reads. The different modes can be selected with `--amplicon`, `--metagenome`, or `--target_capture` respectively. ## Samplesheet input From 29956270d528a18c80f8e96256934c471e5f165c Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:31:27 +0800 Subject: [PATCH 17/89] Update docs/usage.md Co-authored-by: Friederike Hanssen --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index a519d7b..3ca37ee 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -44,7 +44,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/readsimulator --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker <--amplicon/--target_capture/--metgenome> +nextflow run nf-core/readsimulator --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker <--amplicon/--target_capture/--metagenome> ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. From ae35f42e21eac67c0e24c2f0211997b76145dc73 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:32:27 +0800 Subject: [PATCH 18/89] Update modules/local/crabs_dbimport.nf Co-authored-by: Friederike Hanssen --- modules/local/crabs_dbimport.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/modules/local/crabs_dbimport.nf b/modules/local/crabs_dbimport.nf index 1775e05..77fa653 100644 --- a/modules/local/crabs_dbimport.nf +++ b/modules/local/crabs_dbimport.nf @@ -1,5 +1,4 @@ process CRABS_DBIMPORT { - tag "$meta.id" label 'process_medium' From 846f9f8ce987dababcf27e22d130a4b9da43ad73 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:32:46 +0800 Subject: [PATCH 19/89] Update modules/local/crabs_insilicopcr.nf Co-authored-by: Friederike Hanssen --- modules/local/crabs_insilicopcr.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/modules/local/crabs_insilicopcr.nf b/modules/local/crabs_insilicopcr.nf index 1f38b52..3f1efe9 100644 --- a/modules/local/crabs_insilicopcr.nf +++ b/modules/local/crabs_insilicopcr.nf @@ -1,5 +1,4 @@ process CRABS_INSILICOPCR { - tag "$meta.id" label 'process_medium' From 3ddb4c0b438b47db3dba9d54c6b2e6e0c6cef703 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:34:31 +0800 Subject: [PATCH 20/89] Update modules/local/insilicoseq_generate.nf Co-authored-by: Friederike Hanssen --- modules/local/insilicoseq_generate.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/modules/local/insilicoseq_generate.nf b/modules/local/insilicoseq_generate.nf index 14205a7..633c5a0 100644 --- a/modules/local/insilicoseq_generate.nf +++ b/modules/local/insilicoseq_generate.nf @@ -1,5 +1,4 @@ process INSILICOSEQ_GENERATE { - tag "$meta.id" label 'process_single' From 2a2946d785616645ca7c3bacae9019b5ef56fc43 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:36:55 +0800 Subject: [PATCH 21/89] Update modules/local/japsa_capsim.nf Co-authored-by: Friederike Hanssen --- modules/local/japsa_capsim.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/modules/local/japsa_capsim.nf b/modules/local/japsa_capsim.nf index 00a7bbb..26d277e 100644 --- a/modules/local/japsa_capsim.nf +++ b/modules/local/japsa_capsim.nf @@ -1,5 +1,4 @@ process JAPSA_CAPSIM { - tag "$meta.id" label 'process_single' From 7f691ce1c20104bf0392e58e658809a89ebba6f3 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:37:20 +0800 Subject: [PATCH 22/89] Update modules/local/merge_samplesheets.nf Co-authored-by: Friederike Hanssen --- modules/local/merge_samplesheets.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/modules/local/merge_samplesheets.nf b/modules/local/merge_samplesheets.nf index 1bd3d98..5572930 100644 --- a/modules/local/merge_samplesheets.nf +++ b/modules/local/merge_samplesheets.nf @@ -1,5 +1,4 @@ process MERGE_SAMPLESHEETS { - tag "$meta.id" label 'process_low' From 7f442d364e97f526e9c7d11f06f476aa8f50367c Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:37:48 +0800 Subject: [PATCH 23/89] Update modules/local/merge_samplesheets.nf Co-authored-by: Friederike Hanssen --- modules/local/merge_samplesheets.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/merge_samplesheets.nf b/modules/local/merge_samplesheets.nf index 5572930..af29c32 100644 --- a/modules/local/merge_samplesheets.nf +++ b/modules/local/merge_samplesheets.nf @@ -1,6 +1,6 @@ process MERGE_SAMPLESHEETS { tag "$meta.id" - label 'process_low' + label 'process_single' conda "conda-forge::sed=4.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? From 050d8573d1e4863e23eadb98fc7b3832b1609a69 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:38:10 +0800 Subject: [PATCH 24/89] Update modules/local/unzip.nf Co-authored-by: Friederike Hanssen --- modules/local/unzip.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/modules/local/unzip.nf b/modules/local/unzip.nf index e69fe9f..a571323 100644 --- a/modules/local/unzip.nf +++ b/modules/local/unzip.nf @@ -1,5 +1,4 @@ process UNZIP { - tag "$file" label 'process_single' From da1f2ece08dde2d1b8a01798516eea907a539024 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:39:22 +0800 Subject: [PATCH 25/89] Update subworkflows/local/amplicon_workflow.nf Co-authored-by: Friederike Hanssen --- subworkflows/local/amplicon_workflow.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index 40694ab..ca9aa47 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -8,7 +8,6 @@ include { CRABS_INSILICOPCR } from '../../modules/local/crabs_insilicopcr' include { ART_ILLUMINA } from '../../modules/nf-core/art/illumina/main' workflow AMPLICON_WORKFLOW { - ch_versions = Channel.empty() take: ch_fasta // file: /path/to/reference.fasta From f0a0143580ae5ff487bb19cac3d7ce40a57ac502 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:39:36 +0800 Subject: [PATCH 26/89] Update subworkflows/local/amplicon_workflow.nf Co-authored-by: Friederike Hanssen --- subworkflows/local/amplicon_workflow.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index ca9aa47..2038207 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -15,7 +15,7 @@ workflow AMPLICON_WORKFLOW { main: ch_ref_fasta = Channel.empty() - +ch_versions = Channel.empty() // // MODULE: Run Crabs db_download if user doesn't have a reference database // From 67d9940d43fc89ac0fb513bdf7aa0d926dfa7225 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:40:00 +0800 Subject: [PATCH 27/89] Update subworkflows/local/amplicon_workflow.nf Co-authored-by: Friederike Hanssen --- subworkflows/local/amplicon_workflow.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index 2038207..dbccf39 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -20,7 +20,7 @@ ch_versions = Channel.empty() // MODULE: Run Crabs db_download if user doesn't have a reference database // if ( !params.fasta ) { - CRABS_DBDOWNLOAD () + CRABS_DBDOWNLOAD() ch_versions = ch_versions.mix(CRABS_DBDOWNLOAD.out.versions) ch_ref_fasta = CRABS_DBDOWNLOAD.out.fasta .map { From 405687795ae725b71c60d94ca5e6f6bc1e138559 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Wed, 1 Nov 2023 10:40:40 +0800 Subject: [PATCH 28/89] Update subworkflows/local/amplicon_workflow.nf Co-authored-by: Friederike Hanssen --- subworkflows/local/amplicon_workflow.nf | 4 +--- 1 file changed, 1 insertion(+), 3 deletions(-) diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index dbccf39..2bec56c 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -25,9 +25,7 @@ ch_versions = Channel.empty() ch_ref_fasta = CRABS_DBDOWNLOAD.out.fasta .map { fasta -> - def meta = [:] - meta.id = "amplicon" - return [ meta, fasta ] + return [ [id:"amplicon"], fasta ] } // From 26d407f1a997581ef7adff1638ced3589c3b449c Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Tue, 7 Nov 2023 01:55:24 +0000 Subject: [PATCH 29/89] Made changes based on pseudo pr comments and added wholegenome option --- CHANGELOG.md | 3 +- CITATIONS.md | 50 +++++++++++++ README.md | 34 ++++++++- conf/modules.config | 39 +++++++++- conf/test.config | 3 +- conf/test_full.config | 8 +- docs/output.md | 25 ++++--- docs/usage.md | 2 +- modules/local/crabs_dbimport.nf | 16 +++- modules/local/create_samplesheet.nf | 3 - modules/local/insilicoseq_generate.nf | 18 +++-- modules/local/unzip.nf | 2 + modules/local/wgsim.nf | 45 +++++++++++ nextflow.config | 15 ++++ nextflow_schema.json | 75 +++++++++++++++++++ subworkflows/local/amplicon_workflow.nf | 11 ++- subworkflows/local/target_capture_workflow.nf | 4 +- workflows/readsimulator.nf | 20 +++++ 18 files changed, 330 insertions(+), 43 deletions(-) create mode 100644 modules/local/wgsim.nf diff --git a/CHANGELOG.md b/CHANGELOG.md index 85346ed..a4de2b7 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,8 +10,9 @@ Initial release of nf-core/readsimulator, created with the [nf-core](https://nf- ### `Added` - Option to simulate amplicon sequencing reads -- Option to simulate metagenomic sequencing reads - Option to simulate target capture sequencing reads +- Option to simulate metagenomic sequencing reads +- Option to simulate wholegenome sequencing reads - Samplesheet creation after reads have been simulated ### `Fixed` diff --git a/CITATIONS.md b/CITATIONS.md index 4fad81b..f59d49d 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -42,6 +42,56 @@ > Twelve years of SAMtools and BCFtools. Petr Danecek, James K Bonfield, Jennifer Liddle, John Marshall, Valeriu Ohan, Martin O Pollard, Andrew Whitwham, Thomas Keane, Shane A McCarthy, Robert M Davies, Heng Li. GigaScience, Volume 10, Issue 2, February 2021, giab008, https://doi.org/10.1093/gigascience/giab008 +- [Wgsim](https://github.com/lh3/wgsim) + +## Reference probe/baitset databases + +All reference probe databases are sourced from [ultraconserved](https://www.ultraconserved.org/) + +- [Tetrapods; 2,560 baits for 2,386 UCEs; version 1](https://academic.oup.com/sysbio/article/61/5/717/1735316) + + > Brant C. Faircloth, John E. McCormack, Nicholas G. Crawford, Michael G. Harvey, Robb T. Brumfield, Travis C. Glenn, Ultraconserved Elements Anchor Thousands of Genetic Markers Spanning Multiple Evolutionary Timescales, Systematic Biology, Volume 61, Issue 5, October 2012, Pages 717–726, https://doi.org/10.1093/sysbio/sys004 + +- [Tetrapods; 5,472 baits for 5,060 UCEs; version 1](https://royalsocietypublishing.org/doi/10.1098/rspb.2014.0823) + + > Sun Keping, Meiklejohn Kelly A., Faircloth Brant C., Glenn Travis C., Braun Edward L. and Kimball Rebecca T., 2014 The evolution of peafowl and other taxa with ocelli (eyespots): a phylogenomic approach. Proc. R. Soc. B. 281: 20140823. 20140823. http://doi.org/10.1098/rspb.2014.0823 + +- [Actinopterygians; 2,001 baits for 500 UCEs; version 1](https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0065923) + + > Faircloth BC, Sorenson L, Santini F, Alfaro ME (2013) A Phylogenomic Perspective on the Radiation of Ray-Finned Fishes Based upon Targeted Sequencing of Ultraconserved Elements (UCEs). PLOS ONE 8(6): e65923. https://doi.org/10.1371/journal.pone.0065923 + +- [Acanthomorphs; 2,628 baits for 1,314 UCEs; version 1](https://royalsocietypublishing.org/doi/10.1098/rspb.2015.1413) + + > McGee Matthew D., Faircloth Brant C., Borstein Samuel R., Zheng Jimmy, Darrin Hulsey C., Wainwright Peter C. and Alfaro Michael E.. 2016 Replicated divergence in cichlid radiations mirrors a major vertebrate innovation. Proc. R. Soc. B. 283: 20151413. 20151413. http://doi.org/10.1098/rspb.2015.1413 + +- [Arachnida; 14,799 baits for 1,120 UCEs; version 1](https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12621) + + > Starrett, J., Derkarabetian, S., Hedin, M., Bryson Jr. R. W., McCormack. J. E., & Faircloth. B. C. (2016). High phylogenetic utility of an Ultraconserved element probe set designed for Arachnida. Molecular Ecology Resources. 17(4), 812-823. https://doi.org/10.1111/1755-0998.12621 + +- [Coleoptera; 13,674 baits for 1,172 UCEs; version 1](https://resjournals.onlinelibrary.wiley.com/doi/10.1111/syen.12244) + + > Baca. S. M., Alexander. A., Gustafson. G. T., & Short. A. E. Z. (2017). Ultraconserved elements show utility in phylogenetic inference of Adephaga (Coleoptera) and suggest paraphyly of Hydradephaga. 42(4), 786-795. https://doi.org/10.1111/syen.12244 + +- [Diptera; 31,328 baits for 2,711 UCEs; version 1](https://besjournals.onlinelibrary.wiley.com/doi/10.1111/2041-210X.12754) + + > Faircloth. B. C. (2017). Identifying conserved genomic elements and designing universal bait sets to enrich them. Methods in Ecology and Evolution. 8(9), 1103-1112. https://doi.org/10.1111/2041-210X.12754 + +- [Hemiptera; 40,207 baits for 2,731 UCEs; version 1](https://besjournals.onlinelibrary.wiley.com/doi/10.1111/2041-210X.12754) + + > Faircloth. B. C. (2017). Identifying conserved genomic elements and designing universal bait sets to enrich them. Methods in Ecology and Evolution. 8(9), 1103-1112. https://doi.org/10.1111/2041-210X.12754 + +- [Hymenoptera; 2,749 baits for 1,510 UCEs; version 1](https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12328) + + > Faircloth. B. C., Branstetter. M. G., White. N. D., & Brady. S. G. (2014). Target enrichment of ultraconserved elements from anthropods provides a genomic perspective on elationships among Hymenoptera. Molecular Ecology Resources. 15(3), 489-501. https://doi.org/10.1111/1755-0998.12328 + +- [Hymenoptera; 31,829 baits for 2,590 UCEs; version 2](https://besjournals.onlinelibrary.wiley.com/doi/10.1111/2041-210X.12742) + + > Branstetter. M. G., Longino. J. T., Ward. P. S., & Faircloth. B. C. (2017). Enriching the ant tree of life: enhanced UCE bait set for genome-scale phylogenetics of ants and other Hymenoptera. 8(6), 768-776. https://doi.org/10.1111.2041-210X.12742 + +- [Anthozoa; 16,306 baits for 720 UCEs and 1,071 exons; version 1](https://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12736) + + > Quattrini. A. M., Faircloth. B. C., Duenas. L. F., Bridge. T. C. L., Brugler. M. R., Calixto-Botia. I. F., DeLeo. D. M., Foret. S., Herrera. S., Lee. S. M. Y., Miller. D. J., Prada. C., Radis-Baptista. G., Ramirez-Portilla. C., Sanchez. J. A., Rodriguez. E., & McFadden. C. S. (2017). Universal target-enrichment baits for anthozoan (Cnidaria) phylogenomics: New approaches to long-standing problems. Molecular Ecology Resources. 18(2), 281-295. https://doi.org/10.1111/1755-0998.12736 + ## Software packaging/containerisation tools - [Anaconda](https://anaconda.com) diff --git a/README.md b/README.md index 402afae..29fe0b0 100644 --- a/README.md +++ b/README.md @@ -12,14 +12,42 @@ ## Introduction -**nf-core/readsimulator** is a pipeline to simulate sequencing reads. The pipeline currently supports simulating Amplicon, Metagenomic, and Target Capture data. It takes a samplesheet with sample names and seeds for random generation to produce simulated fastq files and a samplesheet that contains the paths to the fastq files. +**nf-core/readsimulator** is a pipeline to simulate sequencing reads. The pipeline currently supports simulating Amplicon, Target Capture, Metagenome, and Wholegenome data. It takes a samplesheet with sample names and seeds for random generation to produce simulated fastq files and a samplesheet that contains the paths to the fastq files. -1. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -2. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) +### Amplicon simulation steps + +1. Create Reference database for Amplicon-Based Sequencing ([`CRABS`](https://github.com/gjeunen/reference_database_creator)) +2. Simulate amplicon Illumina reads ([`art_illumina`](https://manpages.debian.org/testing/art-nextgen-simulation-tools/art_illumina.1.en.html)) +3. Create samplesheet with sample names and paths to simluated read files +4. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +5. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) + +### Target capture simulation steps + +1. Align probes to genome ([`Bowtie2`](https://bowtie-bio.sourceforge.net/bowtie2/index.shtml)) +2. Get SAM index ([`SAMtools`](https://www.htslib.org/)) +3. Simulate target capture reads (Illumina or Pacbio; default=Illumina) ([`Japsa capsim`](https://japsa.readthedocs.io/en/latest/tools/jsa.sim.capsim.html)) +4. Create samplesheet with sample names and paths to simluated read files +5. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +6. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) + +### Metagenome simulation steps + +1. Simulate metagenome Illumina reads ([`InsilicoSeq Generate`](https://insilicoseq.readthedocs.io/en/latest/)) +2. Create samplesheet with sample names and paths to simluated read files +3. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +4. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) + +### Wholegenome simulation steps + +1. Simulate wholegenome reads ([`wgsim`](https://github.com/lh3/wgsim)) +2. Create samplesheet with sample names and paths to simluated read files +3. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) +4. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) ## Usage diff --git a/conf/modules.config b/conf/modules.config index 05c7945..6b07b1c 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -19,7 +19,7 @@ process { ] withName: ART_ILLUMINA { - ext.args = "-amp -p -na -c 500" + ext.args = "-amp -p -na -c ${params.amplicon_read_count}" publishDir = [ path: { "${params.outdir}/art_illumina" }, mode: params.publish_dir_mode @@ -93,7 +93,8 @@ process { withName: CREATE_SAMPLESHEET { publishDir = [ path: { "${params.outdir}/samplesheet_individual_samples" }, - mode: params.publish_dir_mode + mode: params.publish_dir_mode, + enabled: false ] } @@ -132,10 +133,44 @@ process { ] } + withName: SAMTOOLS_INDEX { + publishDir = [ + path: { "${params.outdir}/bowtie2" }, + mode: params.publish_dir_mode + ] + } + withName: UNZIP { publishDir = [ path: { "${params.outdir}/probes" }, mode: params.publish_dir_mode ] } + + withName: GUNZIP { + publishDir = [ + path: { "${params.outdir}/gunzip" }, + mode: params.publish_dir_mode, + enabled: false + ] + } + + withName: WGSIM { + ext.args = [ + "-e ${params.wholegenome_error_rate}", + "-d ${params.wholegenome_outer_dist}", + "-s ${params.wholegenome_standard_dev}", + "-N ${params.wholegenome_n_reads}", + "-1 ${params.wholegenome_r1_length}", + "-2 ${params.wholegenome_r2_length}", + "-r ${params.wholegenome_mutation_rate}", + "-R ${params.wholegenome_indel_fraction}", + "-X ${params.wholegenome_indel_extended}" + ].join(' ').trim() + + publishDir = [ + path: { "${params.outdir}/wgsim" }, + mode: params.publish_dir_mode + ] + } } diff --git a/conf/test.config b/conf/test.config index 0793c09..dcb01e8 100644 --- a/conf/test.config +++ b/conf/test.config @@ -23,6 +23,5 @@ params { input = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/samplesheet/test_samplesheet.csv' fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/testdata/GCF_024334085.1_ASM2433408v1_genomic.fna.gz' - target_capture = true - probe_ref_name = 'Diptera-2.7Kv1' + wholegenome = true } diff --git a/conf/test_full.config b/conf/test_full.config index 5bc8140..3e6af03 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -16,10 +16,14 @@ params { // Input data for full size test input = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/samplesheet/test_samplesheet.csv' - fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/Sarek/testdata/hg38.chrM.fa' + //fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/taxprofiler/data/fasta/ERX5474930_ERR5766174_1.fa.gz' + fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/testdata/GCF_024334085.1_ASM2433408v1_genomic.fna.gz' metagenome = true metagenome_n_genomes = '1' - metagenome_n_reads = '10' + metagenome_n_reads = '100' amplicon = true + target_capture = true + probe_ref_name = 'Diptera-2.7Kv1' + wholegenome = true } diff --git a/docs/output.md b/docs/output.md index c8bae09..b8efcc5 100644 --- a/docs/output.md +++ b/docs/output.md @@ -11,7 +11,7 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: - [ART](#art) - ART Illumina simulated reads -- [Bowtie2](#bowtie2) - Bowtie2 alignments +- [Bowtie2](#bowtie2) - Bowtie2 alignments and SAMtools index - [CapSim](#capsim) - CapSim simulated reads (Illumina or Pacbio) - [CRABS](#crabs) - Formated reference database for simulating amplicon reads - [FastQC](#fastqc) - Raw read QC @@ -19,8 +19,8 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution - [Samplesheet](#samplesheet) - Samplesheets produced during the running of the pipeline -- [SAMtools](#samtools) - SAMtools index - [Unzip](#unzip) - Unziped probe file +- [Wgsim](#wgsim) - Wgsim simulated wholegenome reads ### ART @@ -45,10 +45,12 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - `*.bt2`: Bowtie2 index files. - `*.bam`: BAM file produced from aligning with Bowtie2. - `*.bowtie2.log`: Log file containing alignment information. + - `*.bai`: Index file produced with SAMtools. [Bowtie2](https://www.nature.com/articles/nmeth.1923) is a popular tool for aligning sequences to reference reads. For further reading and documentation see the [Bowtie2 manual](https://bowtie-bio.sourceforge.net/bowtie2/manual.shtml). +[SAMtools](https://academic.oup.com/gigascience/article/10/2/giab008/6137722?login=false) is a popular set of tools for working with sequencing data. For further reading and documentation see the [SAMtools documentation](http://www.htslib.org/doc/). ### CapSim @@ -158,25 +160,26 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ -### SAMtools +### Unzip
    Output files -- `samtools/` - - `*.bai`: Index file produced with SAMtools. +- `probes/` + - `unziped/` + - `*.fasta`: Probe file downloaded if custom probe hasn't been provided with `--probe_fasta` parameter.
    -[SAMtools](https://academic.oup.com/gigascience/article/10/2/giab008/6137722?login=false) is a popular set of tools for working with sequencing data. For further reading and documentation see the [SAMtools documentation](http://www.htslib.org/doc/). - -### Unzip +### Wgsim
    Output files -- `probes/` - - `unziped/` - - `*.fasta`: Probe file downloaded if custom probe hasn't been provided with `--probe_fasta` parameter. +- `wgsim/` + - `*R1.fq.gz`: Read 1 files simulating wholegenome reads. The prefix will be the sample name found in the samplesheet. + - `*R2.fq.gz`: Read 2 files simulating wholegenome reads. The prefix will be the sample name found in the samplesheet.
    + +[Wgsim](https://github.com/lh3/wgsim) is a tool for simulating wholegenome sequencing reads. For further reading and documentation see the [Wgsim manual](https://www.venea.net/man/wgsim(1)). diff --git a/docs/usage.md b/docs/usage.md index 3ca37ee..f7345bc 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -6,7 +6,7 @@ ## Introduction -The pipeline currently supporst simulating amplicon, metagenomic, and target capture sequencing reads. The different modes can be selected with `--amplicon`, `--metagenome`, or `--target_capture` respectively. +The pipeline currently supports simulating amplicon, target capture, metagenome, and wholegenome sequencing reads. The different modes can be selected with `--amplicon`, `--target_capture`, `--metagenome`, or `--wholegenome` respectively. ## Samplesheet input diff --git a/modules/local/crabs_dbimport.nf b/modules/local/crabs_dbimport.nf index 77fa653..67f6b97 100644 --- a/modules/local/crabs_dbimport.nf +++ b/modules/local/crabs_dbimport.nf @@ -19,15 +19,23 @@ process CRABS_DBIMPORT { task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def VERSION = '0.1.1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def VERSION = '0.1.1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + def is_compressed = fasta.name.endsWith(".gz") + def fasta_name = fasta.name.replace(".gz", "") """ + if [ "${is_compressed}" == "true" ]; then + gzip -c -d ${fasta} > ${fasta_name} + fi + crabs db_import \\ - --input $fasta \\ + --input ${fasta_name} \\ --output ${prefix}.crabsdb.fa \\ $args + rm ${fasta_name} + cat <<-END_VERSIONS > versions.yml "${task.process}": crabs: $VERSION diff --git a/modules/local/create_samplesheet.nf b/modules/local/create_samplesheet.nf index c48c0f3..efbb002 100644 --- a/modules/local/create_samplesheet.nf +++ b/modules/local/create_samplesheet.nf @@ -1,9 +1,6 @@ process CREATE_SAMPLESHEET { tag "$meta.id" - executor 'local' - memory 100.MB - input: tuple val(meta), path(fastq) diff --git a/modules/local/insilicoseq_generate.nf b/modules/local/insilicoseq_generate.nf index 633c5a0..4847ed8 100644 --- a/modules/local/insilicoseq_generate.nf +++ b/modules/local/insilicoseq_generate.nf @@ -19,23 +19,31 @@ process INSILICOSEQ_GENERATE { task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def seed = task.ext.seed ?: "${meta.seed}" + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def seed = task.ext.seed ?: "${meta.seed}" if (fasta) { + def is_compressed = fasta.name.endsWith(".gz") + def fasta_name = fasta.name.replace(".gz", "") """ seed=\$(echo $seed | sed 's/\\[//g' | sed 's/\\]//g') prefix=\$(echo $prefix | sed 's/\\[//g' | sed 's/\\]//g') + if [ "${is_compressed}" == "true" ]; then + gzip -c -d ${fasta} > ${fasta_name} + fi + iss generate \\ - --genomes $fasta \\ + --genomes ${fasta_name} \\ --seed \$seed \\ --output \$prefix \\ --compress \\ --cpus $task.cpus \\ $args + rm ${fasta_name} + cat <<-END_VERSIONS > versions.yml "${task.process}": insilicoseq: \$(iss --version | sed 's/iss version //g') diff --git a/modules/local/unzip.nf b/modules/local/unzip.nf index a571323..554d8f6 100644 --- a/modules/local/unzip.nf +++ b/modules/local/unzip.nf @@ -9,8 +9,10 @@ process UNZIP { input: path(file) + //tuple val(meta), path(file) output: + //tuple val(meta), path "unziped/*", emit: file path "unziped/*" , emit: file path "versions.yml", emit: versions diff --git a/modules/local/wgsim.nf b/modules/local/wgsim.nf new file mode 100644 index 0000000..607818b --- /dev/null +++ b/modules/local/wgsim.nf @@ -0,0 +1,45 @@ +process WGSIM { + tag "$meta.id" + label 'process_single' + + // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. + conda "bioconda::wgsim=1.0" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/wgsim:1.0--he4a0461_7': + 'biocontainers/wgsim:1.0--he4a0461_7' }" + + input: + path(fasta) + val(meta) + + output: + tuple val(meta), path("*.fq.gz*"), emit: fastq + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def seed = task.ext.seed ?: "${meta.seed}" + def VERSION = '1.0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + seed=\$(echo $seed | sed 's/\\[//g' | sed 's/\\]//g') + prefix=\$(echo $prefix | sed 's/\\[//g' | sed 's/\\]//g') + + wgsim \\ + $args \\ + -S \$seed \\ + $fasta \\ + \${prefix}_R1.fq \\ + \${prefix}_R2.fq + + gzip \${prefix}_R1.fq \${prefix}_R2.fq + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + wgsim: $VERSION + END_VERSIONS + """ +} diff --git a/nextflow.config b/nextflow.config index 213331e..42a6b72 100644 --- a/nextflow.config +++ b/nextflow.config @@ -21,6 +21,7 @@ params { amplicon = null target_capture = null metagenome = null + wholegenome = null // Amplicon options fw_primer = 'GTCGGTAAAACTCGTGCCAGC' @@ -33,6 +34,9 @@ params { amplicon_ncbi_batchsize = 5000 amplicon_embl_db = 'vrt*' amplicon_bold_db = 'Actinopterygii|Aves' + amplicon_read_count = 500 + amplicon_read_length = 130 + amplicon_seq_system = 'HS25' // Target capture options target_capture_pacbio = null @@ -51,6 +55,17 @@ params { metagenome_model = 'MiSeq' metagenome_gc_bias = null + // Whole Genome options + wholegenome_error_rate = 0.020 + wholegenome_outer_dist = 500 + wholegenome_standard_dev = 50 + wholegenome_n_reads = 1000000 + wholegenome_r1_length = 70 + wholegenome_r2_length = 70 + wholegenome_mutation_rate = 0.0010 + wholegenome_indel_fraction = 0.15 + wholegenome_indel_extended = 0.30 + // MultiQC options multiqc_config = null multiqc_title = null diff --git a/nextflow_schema.json b/nextflow_schema.json index a524295..27bd6e0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -63,6 +63,11 @@ "type": "boolean", "description": "Option to simulate metagenomic data.", "fa_icon": "fas fa-question-circle" + }, + "wholegenome": { + "type": "boolean", + "description": "Option to simulate wholegenomic data.", + "fa_icon": "fas fa-question-circle" } } }, @@ -123,6 +128,23 @@ "type": "string", "description": "BOLD database to use when `--amplicon_ref_source` is set to 'bold', seperate databases with '|'", "default": "Actinopterygii|Aves" + }, + "amplicon_read_count": { + "type": "integer", + "description": "Number of reads to be simulated.", + "default": 500 + }, + "amplicon_read_length": { + "type": "integer", + "description": "Length of reads to be simulated.", + "default": 130 + }, + "amplicon_seq_system": { + "type": "string", + "description": "Sequencing system of reads to be simulated.", + "default": "HS25", + "help_text": "Can be 'GA1' for Genome Analyser I, 'GA2' for Genome Analyser II, 'HS10' for HiSeq 1000, 'HS20' for HiSeq 2000, 'HS25' for HiSeq 2500, or 'MS' for MiSeq.", + "enum": ["GA1", "GA2", "HS10", "HS20", "HS25", "MS"] } } }, @@ -242,6 +264,59 @@ } } }, + "wholegenome_options": { + "title": "Wholegenome options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Options for simulating wholegenome data.", + "properties": { + "wholegenome_error_rate": { + "type": "number", + "default": 0.020, + "description": "The base error rate." + }, + "wholegenome_outer_dist": { + "type": "integer", + "default": 500, + "description": "The outer distance between the two ends." + }, + "wholegenome_standard_dev": { + "type": "integer", + "default": 50, + "description": "The standard deviation." + }, + "wholegenome_n_reads": { + "type": "integer", + "default": 1000000, + "description": "The number of read pairs." + }, + "wholegenome_r1_length": { + "type": "integer", + "default": 70, + "description": "The length of the first reads." + }, + "wholegenome_r2_length": { + "type": "integer", + "default": 70, + "description": "The length of the second reads." + }, + "wholegenome_mutation_rate": { + "type": "number", + "default": 0.0010, + "description": "The rate of mutations." + }, + "wholegenome_indel_fraction": { + "type": "number", + "default": 0.15, + "description": "The fraction of indels." + }, + "wholegenome_indel_extended": { + "type": "number", + "default": 0.30, + "description": "The probability that an indel is extended." + } + } + }, "reference_genome_options": { "title": "Reference genome options", "type": "object", diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index 2bec56c..b109c28 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -15,7 +15,8 @@ workflow AMPLICON_WORKFLOW { main: ch_ref_fasta = Channel.empty() -ch_versions = Channel.empty() + ch_versions = Channel.empty() + // // MODULE: Run Crabs db_download if user doesn't have a reference database // @@ -35,9 +36,7 @@ ch_versions = Channel.empty() ch_meta_fasta = Channel.fromPath(params.fasta) .map { fasta -> - def meta = [:] - meta.id = "amplicon" - return [ meta, fasta ] + return [ [id:"amplicon"], fasta ] } CRABS_DBIMPORT ( @@ -68,8 +67,8 @@ ch_versions = Channel.empty() // ART_ILLUMINA ( ch_art_input, - "HS25", - 130 + params.amplicon_seq_system, + params.amplicon_read_length ) ch_versions = ch_versions.mix(ART_ILLUMINA.out.versions.first()) diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index f3c8a42..8172800 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -21,9 +21,7 @@ workflow TARGET_CAPTURE_WORKFLOW { ch_meta_fasta = ch_fasta .map { fasta -> - def meta = [:] - meta.id = "target_capture" - return [ meta, fasta ] + return [ [id:"target_capture"], fasta ] } // diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 5615630..4d74ec2 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -38,6 +38,7 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil include { INSILICOSEQ_GENERATE } from '../modules/local/insilicoseq_generate' include { CREATE_SAMPLESHEET } from '../modules/local/create_samplesheet' include { MERGE_SAMPLESHEETS } from '../modules/local/merge_samplesheets' +include { WGSIM } from '../modules/local/wgsim' // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules @@ -130,6 +131,25 @@ workflow READSIMULATOR { ch_simulated_reads = ch_simulated_reads.mix(ch_metagenome_reads) } + // + // MODULE: Simulate wholegenomic reads + // + if ( params.wholegenome ) { + WGSIM ( + ch_fasta.first(), + ch_input + ) + ch_versions = ch_versions.mix(WGSIM.out.versions.first()) + ch_wholegenome_reads = WGSIM.out.fastq + .map { + meta, fastqs -> + meta[0].outdir = "wgsim" + meta[0].datatype = "wholegenome" + return [ meta[0], fastqs ] + } + ch_simulated_reads = ch_simulated_reads.mix(ch_wholegenome_reads) + } + // MODULE: Create sample sheet (just the header and one row) CREATE_SAMPLESHEET ( ch_simulated_reads From 53e598b3dc7e5afefada9b15b5695584c5f83d9c Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Tue, 7 Nov 2023 02:28:22 +0000 Subject: [PATCH 30/89] fixed linting issues --- conf/modules.config | 2 +- docs/output.md | 2 +- nextflow_schema.json | 9 ++++++--- 3 files changed, 8 insertions(+), 5 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 6b07b1c..872c667 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -156,7 +156,7 @@ process { } withName: WGSIM { - ext.args = [ + ext.args = [ "-e ${params.wholegenome_error_rate}", "-d ${params.wholegenome_outer_dist}", "-s ${params.wholegenome_standard_dev}", diff --git a/docs/output.md b/docs/output.md index b8efcc5..66f8adb 100644 --- a/docs/output.md +++ b/docs/output.md @@ -182,4 +182,4 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ -[Wgsim](https://github.com/lh3/wgsim) is a tool for simulating wholegenome sequencing reads. For further reading and documentation see the [Wgsim manual](https://www.venea.net/man/wgsim(1)). +[Wgsim](https://github.com/lh3/wgsim) is a tool for simulating wholegenome sequencing reads. For further reading and documentation see the [Wgsim manual](). diff --git a/nextflow_schema.json b/nextflow_schema.json index 27bd6e0..3fcd97c 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -272,7 +272,7 @@ "properties": { "wholegenome_error_rate": { "type": "number", - "default": 0.020, + "default": 0.02, "description": "The base error rate." }, "wholegenome_outer_dist": { @@ -302,7 +302,7 @@ }, "wholegenome_mutation_rate": { "type": "number", - "default": 0.0010, + "default": 0.001, "description": "The rate of mutations." }, "wholegenome_indel_fraction": { @@ -312,7 +312,7 @@ }, "wholegenome_indel_extended": { "type": "number", - "default": 0.30, + "default": 0.3, "description": "The probability that an indel is extended." } } @@ -574,6 +574,9 @@ { "$ref": "#/definitions/metagenome_options" }, + { + "$ref": "#/definitions/wholegenome_options" + }, { "$ref": "#/definitions/reference_genome_options" }, From 95c20bac2cb9e1d63588a9a4b3b80149c89cdb96 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 8 Nov 2023 00:26:03 +0000 Subject: [PATCH 31/89] Moved conda exit code to script declaration --- modules/local/japsa_capsim.nf | 9 ++++----- 1 file changed, 4 insertions(+), 5 deletions(-) diff --git a/modules/local/japsa_capsim.nf b/modules/local/japsa_capsim.nf index 26d277e..009c144 100644 --- a/modules/local/japsa_capsim.nf +++ b/modules/local/japsa_capsim.nf @@ -4,11 +4,6 @@ process JAPSA_CAPSIM { container "docker.io/vmurigneux/japsa@sha256:ba74e9c844d115f390be62cde1272cfa5e10492512674ea342a4eeec47840f98" - // Exit if running this module with -profile conda / -profile mamba - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - exit 1, "CAPSIM does not support Conda. Please use Docker / Singularity / Podman instead." - } - input: tuple val(meta), path(fasta), path(probes), path(index) @@ -20,6 +15,10 @@ process JAPSA_CAPSIM { task.ext.when == null || task.ext.when script: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "CAPSIM does not support Conda. Please use Docker / Singularity / Podman instead." + } def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def seed = task.ext.seed ?: "${meta.seed}" From 742b33082279bf5419dd4ff6fe800f82cb843028 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 8 Nov 2023 00:48:59 +0000 Subject: [PATCH 32/89] remove commented out code --- conf/test_full.config | 1 - 1 file changed, 1 deletion(-) diff --git a/conf/test_full.config b/conf/test_full.config index 3e6af03..07a9085 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -16,7 +16,6 @@ params { // Input data for full size test input = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/samplesheet/test_samplesheet.csv' - //fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/taxprofiler/data/fasta/ERX5474930_ERR5766174_1.fa.gz' fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/testdata/GCF_024334085.1_ASM2433408v1_genomic.fna.gz' metagenome = true From 0dbf49c034661e96841d6708f83dd1e92b9ec477 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 8 Nov 2023 11:23:31 +0000 Subject: [PATCH 33/89] Fixed description of amplicon_read_count option --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 3fcd97c..0fcfbc6 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -131,7 +131,7 @@ }, "amplicon_read_count": { "type": "integer", - "description": "Number of reads to be simulated.", + "description": "Number of reads to be simulated per amplicon.", "default": 500 }, "amplicon_read_length": { From f866d3cb4aa42ae0dd432dc042d81f4a2d69869a Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 9 Nov 2023 00:48:16 +0000 Subject: [PATCH 34/89] removed config for module that was already removed --- conf/modules.config | 8 -------- 1 file changed, 8 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 872c667..042960c 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -147,14 +147,6 @@ process { ] } - withName: GUNZIP { - publishDir = [ - path: { "${params.outdir}/gunzip" }, - mode: params.publish_dir_mode, - enabled: false - ] - } - withName: WGSIM { ext.args = [ "-e ${params.wholegenome_error_rate}", From c3256bb8aae5375a45cbb0fbb5bd402d40389ead Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 9 Nov 2023 01:05:54 +0000 Subject: [PATCH 35/89] small fixes the comments/documentation --- README.md | 8 ++++---- conf/base.config | 1 - subworkflows/local/target_capture_workflow.nf | 2 +- 3 files changed, 5 insertions(+), 6 deletions(-) diff --git a/README.md b/README.md index 29fe0b0..f1fa47a 100644 --- a/README.md +++ b/README.md @@ -22,7 +22,7 @@ 1. Create Reference database for Amplicon-Based Sequencing ([`CRABS`](https://github.com/gjeunen/reference_database_creator)) 2. Simulate amplicon Illumina reads ([`art_illumina`](https://manpages.debian.org/testing/art-nextgen-simulation-tools/art_illumina.1.en.html)) -3. Create samplesheet with sample names and paths to simluated read files +3. Create samplesheet with sample names and paths to simulated read files 4. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 5. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) @@ -31,21 +31,21 @@ 1. Align probes to genome ([`Bowtie2`](https://bowtie-bio.sourceforge.net/bowtie2/index.shtml)) 2. Get SAM index ([`SAMtools`](https://www.htslib.org/)) 3. Simulate target capture reads (Illumina or Pacbio; default=Illumina) ([`Japsa capsim`](https://japsa.readthedocs.io/en/latest/tools/jsa.sim.capsim.html)) -4. Create samplesheet with sample names and paths to simluated read files +4. Create samplesheet with sample names and paths to simulated read files 5. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 6. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) ### Metagenome simulation steps 1. Simulate metagenome Illumina reads ([`InsilicoSeq Generate`](https://insilicoseq.readthedocs.io/en/latest/)) -2. Create samplesheet with sample names and paths to simluated read files +2. Create samplesheet with sample names and paths to simulated read files 3. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 4. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) ### Wholegenome simulation steps 1. Simulate wholegenome reads ([`wgsim`](https://github.com/lh3/wgsim)) -2. Create samplesheet with sample names and paths to simluated read files +2. Create samplesheet with sample names and paths to simulated read files 3. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 4. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) diff --git a/conf/base.config b/conf/base.config index 081c2eb..661772d 100644 --- a/conf/base.config +++ b/conf/base.config @@ -10,7 +10,6 @@ process { - // TODO nf-core: Check the defaults for all processes cpus = { check_max( 1 * task.attempt, 'cpus' ) } memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 4.h * task.attempt, 'time' ) } diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index 8172800..187b6f8 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -33,7 +33,7 @@ workflow TARGET_CAPTURE_WORKFLOW { ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions.first()) // - // MODULE: Unzip probes file if user is downloading them + // MODULE: Unzip probes file if user is downloading a reference probe file // if ( !params.probe_fasta ) { ch_zip_file = Channel.fromPath(params.probe_ref_db[params.probe_ref_name]["url"]) From d5f875264289ac0481b0e10adf5f03371b97710e Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Fri, 10 Nov 2023 01:42:58 +0000 Subject: [PATCH 36/89] removed parameter that was buggy with a container and made small changes --- conf/modules.config | 8 +++----- nextflow.config | 8 +------- nextflow_schema.json | 10 ---------- subworkflows/local/target_capture_workflow.nf | 4 ++-- 4 files changed, 6 insertions(+), 24 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 042960c..cf0b07e 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -61,13 +61,11 @@ process { withName: CRABS_DBDOWNLOAD { ext.args = params.amplicon_ref_source == "ncbi" ? - "--source ncbi --database ${params.amplicon_ncbi_db} --query ${params.amplicon_ncbi_query} --species ${params.amplicon_ncbi_species} --email ${params.amplicon_ncbi_email} --batchsize ${params.amplicon_ncbi_batchsize} --keep_original no --output ncbi.fasta" : + "--source ncbi --database ${params.amplicon_ncbi_db} --query '${params.amplicon_ncbi_query}' --email johndoe@gmail.com --batchsize ${params.amplicon_ncbi_batchsize} --keep_original no --output ncbi.fasta" : params.amplicon_ref_source == "embl" ? "--source embl --database ${params.amplicon_embl_db} --output embl.fasta --keep_original no" : params.amplicon_ref_source == "bold" ? - "--source bold --database '${params.amplicon_bold_db}' --keep_original no --output bold.fasta" : - params.amplicon_ref_source == "mitofish" ? - "--source mitofish --keep_original no --output mitofish.fasta" : "" + "--source bold --database '${params.amplicon_bold_db}' --keep_original no --output bold.fasta" : "" publishDir = [ path: { "${params.outdir}/crabs_dbdownload" }, mode: params.publish_dir_mode @@ -140,7 +138,7 @@ process { ] } - withName: UNZIP { + withName: UNZIP_PROBE { publishDir = [ path: { "${params.outdir}/probes" }, mode: params.publish_dir_mode diff --git a/nextflow.config b/nextflow.config index 42a6b72..96238f6 100644 --- a/nextflow.config +++ b/nextflow.config @@ -29,8 +29,6 @@ params { amplicon_ref_source = 'bold' amplicon_ncbi_db = 'nucleotide' amplicon_ncbi_query = '16S[All Fields] AND ("1"[SLEN] : "50000"[SLEN])' - amplicon_ncbi_species = 'Carcharinus leucas + Carcharinus acronotus' - amplicon_ncbi_email = 'johndoe@gmail.com' amplicon_ncbi_batchsize = 5000 amplicon_embl_db = 'vrt*' amplicon_bold_db = 'Actinopterygii|Aves' @@ -107,11 +105,6 @@ params { validate_params = true } -// Set igenomes base to null to avoid unnecessary permission error -if (params.fasta || !params.target_capture) { - params.igenomes_base = null -} - // Load base.config by default for all pipelines includeConfig 'conf/base.config' @@ -243,6 +236,7 @@ if (!params.igenomes_ignore) { includeConfig 'conf/igenomes.config' } else { params.genomes = [:] + params.igenomes_base = null } // Export these variables to prevent local Python/R libraries from conflicting with those in the container // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. diff --git a/nextflow_schema.json b/nextflow_schema.json index 0fcfbc6..1373a06 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -104,16 +104,6 @@ "description": "NCBI query to use when `--amplicon_ref_source` is set to 'ncbi'", "default": "16S[All Fields] AND (\"1\"[SLEN] : \"50000\"[SLEN])" }, - "amplicon_ncbi_species": { - "type": "string", - "description": "Species to use when `--amplicon_ref_source` is set to 'ncbi', seperate taxa with ' + '", - "default": "Carcharinus leucas + Carcharinus acronotus" - }, - "amplicon_ncbi_email": { - "type": "string", - "description": "email to conect to ncbi when `--amplicon_ref_source` is set to 'ncbi'", - "default": "johndoe@gmail.com" - }, "amplicon_ncbi_batchsize": { "type": "integer", "description": "Batchsize to use when `--amplicon_ref_source` is set to 'ncbi'", diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index 187b6f8..e64af1f 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -7,7 +7,7 @@ include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/ include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' include { JAPSA_CAPSIM as CAPSIM_ILLUMINA } from '../../modules/local/japsa_capsim' include { JAPSA_CAPSIM as CAPSIM_PACBIO } from '../../modules/local/japsa_capsim' -include { UNZIP } from '../../modules/local/unzip' +include { UNZIP as UNZIP_PROBE } from '../../modules/local/unzip' workflow TARGET_CAPTURE_WORKFLOW { take: @@ -37,7 +37,7 @@ workflow TARGET_CAPTURE_WORKFLOW { // if ( !params.probe_fasta ) { ch_zip_file = Channel.fromPath(params.probe_ref_db[params.probe_ref_name]["url"]) - ch_probes = UNZIP ( + ch_probes = UNZIP_PROBE ( ch_zip_file ).file } From 033da0a9ca551912b1aff0a8632b43a4292f83d1 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 16 Nov 2023 00:45:17 +0000 Subject: [PATCH 37/89] fixed bug, improved documentation, and improved test_full --- conf/modules.config | 2 +- conf/test_full.config | 17 ++++++++++------- modules/local/insilicoseq_generate.nf | 2 +- nextflow_schema.json | 23 ++++++++++++----------- 4 files changed, 24 insertions(+), 20 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index cf0b07e..bbc7805 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -73,7 +73,7 @@ process { } withName: CRABS_DBIMPORT { - ext.args = "--seq_header accession --delim ' '" + ext.args = "--seq_header species --delim ' '" publishDir = [ path: { "${params.outdir}/crabs_dbimport" }, mode: params.publish_dir_mode diff --git a/conf/test_full.config b/conf/test_full.config index 07a9085..63a0240 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -18,11 +18,14 @@ params { input = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/samplesheet/test_samplesheet.csv' fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/testdata/GCF_024334085.1_ASM2433408v1_genomic.fna.gz' - metagenome = true - metagenome_n_genomes = '1' - metagenome_n_reads = '100' - amplicon = true - target_capture = true - probe_ref_name = 'Diptera-2.7Kv1' - wholegenome = true + amplicon = true + metagenome = true + target_capture = true + wholegenome = true + + fw_primer = 'AAAATAAT' + rv_primer = 'GATTACTTT' + amplicon_read_count = 1000 + metagenome_n_reads = '100K' + probe_ref_name = 'Diptera-2.7Kv1' } diff --git a/modules/local/insilicoseq_generate.nf b/modules/local/insilicoseq_generate.nf index 4847ed8..dfc65be 100644 --- a/modules/local/insilicoseq_generate.nf +++ b/modules/local/insilicoseq_generate.nf @@ -1,6 +1,6 @@ process INSILICOSEQ_GENERATE { tag "$meta.id" - label 'process_single' + label 'process_low' conda "bioconda::insilicoseq=1.6.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? diff --git a/nextflow_schema.json b/nextflow_schema.json index 1373a06..95710e8 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -51,22 +51,22 @@ "properties": { "amplicon": { "type": "boolean", - "description": "Option to simulate amplicon data.", + "description": "Option to simulate amplicon sequencing reads.", "fa_icon": "fas fa-question-circle" }, "target_capture": { "type": "boolean", - "description": "Option to simulate UCE target capture data.", + "description": "Option to simulate target capture sequencing reads.", "fa_icon": "fas fa-question-circle" }, "metagenome": { "type": "boolean", - "description": "Option to simulate metagenomic data.", + "description": "Option to simulate metagenomic sequencing reads.", "fa_icon": "fas fa-question-circle" }, "wholegenome": { "type": "boolean", - "description": "Option to simulate wholegenomic data.", + "description": "Option to simulate wholegenomic sequencing reads.", "fa_icon": "fas fa-question-circle" } } @@ -75,7 +75,7 @@ "title": "Amplicon options", "type": "object", "fa_icon": "fas fa-dna", - "description": "Options for simulating amplicon data.", + "description": "Options for simulating amplicon sequencing reads.", "properties": { "fw_primer": { "type": "string", @@ -102,7 +102,8 @@ "amplicon_ncbi_query": { "type": "string", "description": "NCBI query to use when `--amplicon_ref_source` is set to 'ncbi'", - "default": "16S[All Fields] AND (\"1\"[SLEN] : \"50000\"[SLEN])" + "default": "16S[All Fields] AND (\"1\"[SLEN] : \"50000\"[SLEN])", + "help_text": "Multiple species can be queried like this '(\"Mus musculus\"[Organism] OR \"Salmo salar\"[Organism]) AND 16S[All Fields]'" }, "amplicon_ncbi_batchsize": { "type": "integer", @@ -142,7 +143,7 @@ "title": "Target capture options", "type": "object", "fa_icon": "fas fa-dna", - "description": "Options for simulating target capture data.", + "description": "Options for simulating target capture sequencing reads.", "properties": { "target_capture_pacbio": { "type": "boolean", @@ -184,7 +185,7 @@ "title": "Metagenome options", "type": "object", "fa_icon": "fas fa-dna", - "description": "Options for simulating metagenomic data.", + "description": "Options for simulating metagenomic sequencing reads.", "properties": { "metagenome_ncbi_db": { "type": "string", @@ -258,7 +259,7 @@ "title": "Wholegenome options", "type": "object", "fa_icon": "fas fa-dna", - "description": "Options for simulating wholegenome data.", + "description": "Options for simulating wholegenome sequencing reads.", "properties": { "wholegenome_error_rate": { "type": "number", @@ -325,8 +326,8 @@ "exists": true, "mimetype": "text/plain", "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", - "description": "Path to FASTA genome file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified, unless simulating amplicon data. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "description": "Path to reference FASTA file.", + "help_text": "If this parameter is not used, the pipeline will download a fasta file, either using the `--genome` parameter or by using other parameters specific to individual modes (e.g., the `--amplicon` mode has parameters specific to just that mode.).", "fa_icon": "far fa-file-code" }, "igenomes_base": { From 8b4b86984e99f31d96ef7766e1f21d26889ba1db Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Fri, 17 Nov 2023 10:58:38 +0000 Subject: [PATCH 38/89] changed capsim to use quay.io container --- modules/local/japsa_capsim.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/japsa_capsim.nf b/modules/local/japsa_capsim.nf index 009c144..4d1deea 100644 --- a/modules/local/japsa_capsim.nf +++ b/modules/local/japsa_capsim.nf @@ -2,7 +2,7 @@ process JAPSA_CAPSIM { tag "$meta.id" label 'process_single' - container "docker.io/vmurigneux/japsa@sha256:ba74e9c844d115f390be62cde1272cfa5e10492512674ea342a4eeec47840f98" + container "nf-core/japsa:0" input: tuple val(meta), path(fasta), path(probes), path(index) From 7d399da857c33af34a12d66676b5a27bbbf6717d Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Mon, 20 Nov 2023 02:08:26 +0000 Subject: [PATCH 39/89] Added date and used semantic versioning --- CHANGELOG.md | 2 +- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 3 files changed, 4 insertions(+), 4 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index a4de2b7..2253928 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.0 - [date] +## 1.0.0 - [20/11/2023] Initial release of nf-core/readsimulator, created with the [nf-core](https://nf-co.re/) template. diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 567d0f9..a1a48f5 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/readsimulator + This report has been generated by the nf-core/readsimulator analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-readsimulator-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index 96238f6..a9a08fc 100644 --- a/nextflow.config +++ b/nextflow.config @@ -277,7 +277,7 @@ manifest { description = """A workflow to simulate reads""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.0' + version = '1.0.0' doi = '' } From d65ab7957085ffd744dd25c19dd9217e4689fa2f Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Mon, 20 Nov 2023 02:10:15 +0000 Subject: [PATCH 40/89] Fixed version whitespace --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index a9a08fc..f83b1c3 100644 --- a/nextflow.config +++ b/nextflow.config @@ -277,7 +277,7 @@ manifest { description = """A workflow to simulate reads""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.0.0' + version = '1.0.0' doi = '' } From 659c8e98c97271cecac3d7de7ebfeac37207d9ce Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Mon, 20 Nov 2023 03:21:16 +0000 Subject: [PATCH 41/89] Added workflow diagram --- README.md | 6 +++--- docs/images/readsimulator_workflow.png | Bin 0 -> 63919 bytes 2 files changed, 3 insertions(+), 3 deletions(-) create mode 100755 docs/images/readsimulator_workflow.png diff --git a/README.md b/README.md index f1fa47a..6082ca5 100644 --- a/README.md +++ b/README.md @@ -14,9 +14,9 @@ **nf-core/readsimulator** is a pipeline to simulate sequencing reads. The pipeline currently supports simulating Amplicon, Target Capture, Metagenome, and Wholegenome data. It takes a samplesheet with sample names and seeds for random generation to produce simulated fastq files and a samplesheet that contains the paths to the fastq files. - - +

    + nf-core/readsimulator workflow overview +

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From b739c9939b551a5e6310aec8829c85e8301450f7 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Tue, 21 Nov 2023 00:38:09 +0000 Subject: [PATCH 45/89] Updated nf-core modules --- modules.json | 12 +-- modules/nf-core/bowtie2/align/environment.yml | 9 ++ modules/nf-core/bowtie2/align/main.nf | 2 +- modules/nf-core/bowtie2/align/meta.yml | 3 + modules/nf-core/bowtie2/build/environment.yml | 7 ++ modules/nf-core/bowtie2/build/main.nf | 6 +- modules/nf-core/bowtie2/build/meta.yml | 3 + .../dumpsoftwareversions/environment.yml | 7 ++ .../custom/dumpsoftwareversions/main.nf | 6 +- .../custom/dumpsoftwareversions/meta.yml | 7 +- .../templates/dumpsoftwareversions.py | 3 +- .../dumpsoftwareversions/tests/main.nf.test | 38 ++++++++ .../tests/main.nf.test.snap | 27 ++++++ .../dumpsoftwareversions/tests/tags.yml | 2 + modules/nf-core/fastqc/environment.yml | 7 ++ modules/nf-core/fastqc/main.nf | 12 ++- modules/nf-core/fastqc/meta.yml | 5 ++ modules/nf-core/fastqc/tests/main.nf.test | 41 +++++++++ .../nf-core/fastqc/tests/main.nf.test.snap | 10 +++ modules/nf-core/fastqc/tests/tags.yml | 2 + modules/nf-core/multiqc/environment.yml | 7 ++ modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/multiqc/meta.yml | 11 ++- .../nf-core/samtools/index/environment.yml | 7 ++ modules/nf-core/samtools/index/main.nf | 2 +- modules/nf-core/samtools/index/meta.yml | 4 + .../samtools/index/tests/csi.nextflow.config | 7 ++ .../nf-core/samtools/index/tests/main.nf.test | 87 +++++++++++++++++++ .../samtools/index/tests/main.nf.test.snap | 28 ++++++ modules/nf-core/samtools/index/tests/tags.yml | 2 + 30 files changed, 340 insertions(+), 30 deletions(-) create mode 100644 modules/nf-core/bowtie2/align/environment.yml create mode 100644 modules/nf-core/bowtie2/build/environment.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml create mode 100644 modules/nf-core/fastqc/environment.yml create mode 100644 modules/nf-core/fastqc/tests/main.nf.test create mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 modules/nf-core/multiqc/environment.yml create mode 100644 modules/nf-core/samtools/index/environment.yml create mode 100644 modules/nf-core/samtools/index/tests/csi.nextflow.config create mode 100644 modules/nf-core/samtools/index/tests/main.nf.test create mode 100644 modules/nf-core/samtools/index/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/index/tests/tags.yml diff --git a/modules.json b/modules.json index 330aba5..51e567e 100644 --- a/modules.json +++ b/modules.json @@ -13,32 +13,32 @@ }, "bowtie2/align": { "branch": "master", - "git_sha": "fe54581f8bed20e4c4a51c616c93fd3379d89820", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "bowtie2/build": { "branch": "master", - "git_sha": "6a24fbe314bb2e6fe6306c29a63076ea87e8eb3c", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "76cc4938c1f6ea5c7d83fed1eeffc146787f9543", + "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "f2d63bd5b68925f98f572eed70993d205cc694b7", + "git_sha": "214d575774c172062924ad3564b4f66655600730", "installed_by": ["modules"] }, "samtools/index": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8", "installed_by": ["modules"] } } diff --git a/modules/nf-core/bowtie2/align/environment.yml b/modules/nf-core/bowtie2/align/environment.yml new file mode 100644 index 0000000..afc3ea8 --- /dev/null +++ b/modules/nf-core/bowtie2/align/environment.yml @@ -0,0 +1,9 @@ +name: bowtie2_align +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bowtie2=2.4.4 + - bioconda::samtools=1.16.1 + - conda-forge::pigz=2.6 diff --git a/modules/nf-core/bowtie2/align/main.nf b/modules/nf-core/bowtie2/align/main.nf index a77114d..e67f6a0 100644 --- a/modules/nf-core/bowtie2/align/main.nf +++ b/modules/nf-core/bowtie2/align/main.nf @@ -2,7 +2,7 @@ process BOWTIE2_ALIGN { tag "$meta.id" label "process_high" - conda "bioconda::bowtie2=2.4.4 bioconda::samtools=1.16.1 conda-forge::pigz=2.6" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' : 'biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' }" diff --git a/modules/nf-core/bowtie2/align/meta.yml b/modules/nf-core/bowtie2/align/meta.yml index 60d04c1..e66811d 100644 --- a/modules/nf-core/bowtie2/align/meta.yml +++ b/modules/nf-core/bowtie2/align/meta.yml @@ -65,3 +65,6 @@ output: authors: - "@joseespinosa" - "@drpatelh" +maintainers: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/bowtie2/build/environment.yml b/modules/nf-core/bowtie2/build/environment.yml new file mode 100644 index 0000000..17af3e5 --- /dev/null +++ b/modules/nf-core/bowtie2/build/environment.yml @@ -0,0 +1,7 @@ +name: bowtie2_build +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bowtie2=2.4.5 diff --git a/modules/nf-core/bowtie2/build/main.nf b/modules/nf-core/bowtie2/build/main.nf index 069d9c1..3a90971 100644 --- a/modules/nf-core/bowtie2/build/main.nf +++ b/modules/nf-core/bowtie2/build/main.nf @@ -2,10 +2,10 @@ process BOWTIE2_BUILD { tag "$fasta" label 'process_high' - conda "bioconda::bowtie2=2.4.4" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bowtie2:2.4.4--py39hbb4e92a_0' : - 'biocontainers/bowtie2:2.4.4--py39hbb4e92a_0' }" + 'https://depot.galaxyproject.org/singularity/bowtie2:2.4.5--py39hbb4e92a_0' : + 'biocontainers/bowtie2:2.4.5--py39hbb4e92a_0' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/bowtie2/build/meta.yml b/modules/nf-core/bowtie2/build/meta.yml index 0240224..2d68799 100644 --- a/modules/nf-core/bowtie2/build/meta.yml +++ b/modules/nf-core/bowtie2/build/meta.yml @@ -41,3 +41,6 @@ output: authors: - "@joseespinosa" - "@drpatelh" +maintainers: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml new file mode 100644 index 0000000..f0c63f6 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -0,0 +1,7 @@ +name: custom_dumpsoftwareversions +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.17 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 800a609..7685b33 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : + 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index c32657d..5f15a5f 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,4 +1,4 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: @@ -16,7 +16,6 @@ input: type: file description: YML file containing software versions pattern: "*.yml" - output: - yml: type: file @@ -30,7 +29,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@drpatelh" - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py index e55b8d4..da03340 100755 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -4,11 +4,10 @@ """Provide functions to merge multiple versions.yml files.""" +import yaml import platform from textwrap import dedent -import yaml - def _make_versions_html(versions): """Generate a tabular HTML output of all versions for MultiQC.""" diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test new file mode 100644 index 0000000..eec1db1 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -0,0 +1,38 @@ +nextflow_process { + + name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" + script "../main.nf" + process "CUSTOM_DUMPSOFTWAREVERSIONS" + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "dumpsoftwareversions" + tag "custom/dumpsoftwareversions" + + test("Should run without failures") { + when { + process { + """ + def tool1_version = ''' + TOOL1: + tool1: 0.11.9 + '''.stripIndent() + + def tool2_version = ''' + TOOL2: + tool2: 1.9 + '''.stripIndent() + + input[0] = Channel.of(tool1_version, tool2_version).collectFile() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap new file mode 100644 index 0000000..4274ed5 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -0,0 +1,27 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ], + "1": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "2": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "mqc_yml": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "versions": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "yml": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ] + } + ], + "timestamp": "2023-11-03T14:43:22.157011" + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml new file mode 100644 index 0000000..405aa24 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml @@ -0,0 +1,2 @@ +custom/dumpsoftwareversions: + - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml new file mode 100644 index 0000000..1787b38 --- /dev/null +++ b/modules/nf-core/fastqc/environment.yml @@ -0,0 +1,7 @@ +name: fastqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 9ae5838..50e59f2 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -2,10 +2,10 @@ process FASTQC { tag "$meta.id" label 'process_medium' - conda "bioconda::fastqc=0.11.9" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' : - 'quay.io/biocontainers/fastqc:0.11.9--0' }" + 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : + 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" input: tuple val(meta), path(reads) @@ -29,7 +29,11 @@ process FASTQC { printf "%s %s\\n" $rename_to | while read old_name new_name; do [ -f "\${new_name}" ] || ln -s \$old_name \$new_name done - fastqc $args --threads $task.cpus $renamed_files + + fastqc \\ + $args \\ + --threads $task.cpus \\ + $renamed_files cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml index 4da5bb5..ee5507e 100644 --- a/modules/nf-core/fastqc/meta.yml +++ b/modules/nf-core/fastqc/meta.yml @@ -50,3 +50,8 @@ authors: - "@grst" - "@ewels" - "@FelixKrueger" +maintainers: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test new file mode 100644 index 0000000..6437a14 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -0,0 +1,41 @@ +nextflow_process { + + name "Test Process FASTQC" + script "../main.nf" + process "FASTQC" + tag "modules" + tag "modules_nfcore" + tag "fastqc" + + test("Single-Read") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id: 'test', single_end:true ], + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:

    + // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, + { assert path(process.out.html.get(0).get(1)).getText().contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + ) + } + } +} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap new file mode 100644 index 0000000..636a32c --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -0,0 +1,10 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2023-10-09T23:40:54+0000" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml new file mode 100644 index 0000000..7834294 --- /dev/null +++ b/modules/nf-core/fastqc/tests/tags.yml @@ -0,0 +1,2 @@ +fastqc: + - modules/nf-core/fastqc/** diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml new file mode 100644 index 0000000..d2a9f21 --- /dev/null +++ b/modules/nf-core/multiqc/environment.yml @@ -0,0 +1,7 @@ +name: multiqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.17 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 4b60474..2bbc398 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,10 +1,10 @@ process MULTIQC { label 'process_single' - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : + 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f93b5ee..f1aa660 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,5 +1,5 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json -name: MultiQC +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: - QC @@ -13,7 +13,6 @@ tools: homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ licence: ["GPL-3.0-or-later"] - input: - multiqc_files: type: file @@ -31,7 +30,6 @@ input: type: file description: Optional logo file for MultiQC pattern: "*.{png}" - output: - report: type: file @@ -54,3 +52,8 @@ authors: - "@bunop" - "@drpatelh" - "@jfy133" +maintainers: + - "@abhi18av" + - "@bunop" + - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml new file mode 100644 index 0000000..3c6f95b --- /dev/null +++ b/modules/nf-core/samtools/index/environment.yml @@ -0,0 +1,7 @@ +name: samtools_index +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.17 diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf index 0b20aa4..256bd7c 100644 --- a/modules/nf-core/samtools/index/main.nf +++ b/modules/nf-core/samtools/index/main.nf @@ -2,7 +2,7 @@ process SAMTOOLS_INDEX { tag "$meta.id" label 'process_low' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : 'biocontainers/samtools:1.17--h00cdaf9_0' }" diff --git a/modules/nf-core/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml index 8bd2fa6..01a4ee0 100644 --- a/modules/nf-core/samtools/index/meta.yml +++ b/modules/nf-core/samtools/index/meta.yml @@ -51,3 +51,7 @@ authors: - "@drpatelh" - "@ewels" - "@maxulysse" +maintainers: + - "@drpatelh" + - "@ewels" + - "@maxulysse" diff --git a/modules/nf-core/samtools/index/tests/csi.nextflow.config b/modules/nf-core/samtools/index/tests/csi.nextflow.config new file mode 100644 index 0000000..0ed260e --- /dev/null +++ b/modules/nf-core/samtools/index/tests/csi.nextflow.config @@ -0,0 +1,7 @@ +process { + + withName: SAMTOOLS_INDEX { + ext.args = '-c' + } + +} diff --git a/modules/nf-core/samtools/index/tests/main.nf.test b/modules/nf-core/samtools/index/tests/main.nf.test new file mode 100644 index 0000000..c76a916 --- /dev/null +++ b/modules/nf-core/samtools/index/tests/main.nf.test @@ -0,0 +1,87 @@ +nextflow_process { + + name "Test Process SAMTOOLS_INDEX" + script "../main.nf" + process "SAMTOOLS_INDEX" + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/index" + + test("sarscov2 [BAI]") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.bai).match("bai") }, + { assert path(process.out.versions.get(0)).getText().contains("samtools") } + ) + } + } + + test("homo_sapiens [CRAI]") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.crai).match("crai") }, + { assert path(process.out.versions.get(0)).getText().contains("samtools") } + ) + } + } + + test("homo_sapiens [CSI]") { + + config "./csi.nextflow.config" + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert path(process.out.csi.get(0).get(1)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("samtools") } + ) + } + } +} diff --git a/modules/nf-core/samtools/index/tests/main.nf.test.snap b/modules/nf-core/samtools/index/tests/main.nf.test.snap new file mode 100644 index 0000000..b3baee7 --- /dev/null +++ b/modules/nf-core/samtools/index/tests/main.nf.test.snap @@ -0,0 +1,28 @@ +{ + "crai": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029" + ] + ] + ], + "timestamp": "2023-11-15T15:17:37.30801" + }, + "bai": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4" + ] + ] + ], + "timestamp": "2023-11-15T15:17:30.869234" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/index/tests/tags.yml b/modules/nf-core/samtools/index/tests/tags.yml new file mode 100644 index 0000000..e0f58a7 --- /dev/null +++ b/modules/nf-core/samtools/index/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/index: + - modules/nf-core/samtools/index/** From 2af7dd6b82a13825ddea00e857a0fb3ac0f20677 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Tue, 21 Nov 2023 04:10:29 +0000 Subject: [PATCH 46/89] Made more parameters visible to user --- conf/modules.config | 26 ++++--- modules/local/insilicoseq_generate.nf | 3 +- modules/local/wgsim.nf | 3 +- nextflow.config | 12 +++- nextflow_schema.json | 70 +++++++++++++++++-- subworkflows/local/target_capture_workflow.nf | 40 +++-------- workflows/readsimulator.nf | 21 +++--- 7 files changed, 112 insertions(+), 63 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index bbc7805..db0521b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -42,18 +42,22 @@ process { ] } - withName: CAPSIM_ILLUMINA { - ext.args = "--fmedian 500 --num 500000 --illen 150 --miseq" - publishDir = [ - path: { "${params.outdir}/capsim_illumina" }, - mode: params.publish_dir_mode - ] - } - - withName: CAPSIM_PACBIO { - ext.args = "--fmedian 500 --num 500000 --pacbio" + withName: JAPSA_CAPSIM { + ext.args = [ + "--fmedian ${params.target_capture_fmedian}", + "--fshape ${params.target_capture_fshape}", + "--smedian ${params.target_capture_smedian}", + "--sshape ${params.target_capture_sshape}", + params.target_capture_tmedian ? "--tmedian ${params.target_capture_tmedian}" : "", + params.target_capture_tshape ? "--tshape ${params.target_capture_tshape}" : "", + "--num ${params.target_capture_num}", + params.target_capture_mode == "illumina" ? + "--illen ${params.target_capture_illen}" : "--pblen ${params.target_capture_pblen}", + params.target_capture_mode == "illumina" ? + "--ilmode ${params.target_capture_ilmode} --miseq" : "--pacbio" + ].join(' ').trim() publishDir = [ - path: { "${params.outdir}/capsim_pacbio" }, + path: { "${params.outdir}/capsim" }, mode: params.publish_dir_mode ] } diff --git a/modules/local/insilicoseq_generate.nf b/modules/local/insilicoseq_generate.nf index dfc65be..f37e387 100644 --- a/modules/local/insilicoseq_generate.nf +++ b/modules/local/insilicoseq_generate.nf @@ -8,8 +8,7 @@ process INSILICOSEQ_GENERATE { 'biocontainers/insilicoseq:1.6.0--pyh7cba7a3_0' }" input: - path(fasta) - val(meta) + tuple val(meta), path(fasta) output: tuple val(meta), path("*.fastq.gz*"), emit: fastq diff --git a/modules/local/wgsim.nf b/modules/local/wgsim.nf index 607818b..8a3790e 100644 --- a/modules/local/wgsim.nf +++ b/modules/local/wgsim.nf @@ -9,8 +9,7 @@ process WGSIM { 'biocontainers/wgsim:1.0--he4a0461_7' }" input: - path(fasta) - val(meta) + tuple val(meta), path(fasta) output: tuple val(meta), path("*.fq.gz*"), emit: fastq diff --git a/nextflow.config b/nextflow.config index f83b1c3..231ce3a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -37,9 +37,19 @@ params { amplicon_seq_system = 'HS25' // Target capture options - target_capture_pacbio = null probe_fasta = null probe_ref_name = 'Tetrapods-UCE-5Kv1' + target_capture_mode = 'illumina' + target_capture_fmedian = 500 + target_capture_fshape = 6.0 + target_capture_smedian = 1300 + target_capture_sshape = 6.0 + target_capture_tmedian = null + target_capture_tshape = null + target_capture_num = 500000 + target_capture_illen = 150 + target_capture_pblen = 30000 + target_capture_ilmode = 'pe' // Metagenome options metagenome_ncbi_db = 'viruses' diff --git a/nextflow_schema.json b/nextflow_schema.json index 95710e8..13bc6f7 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -145,11 +145,6 @@ "fa_icon": "fas fa-dna", "description": "Options for simulating target capture sequencing reads.", "properties": { - "target_capture_pacbio": { - "type": "boolean", - "description": "Use this option to simulate Pacbio long-read target capture reads.", - "help_text": "By default, only Illumina short-reads are simulated for target capture data." - }, "probe_fasta": { "type": "string", "format": "file-path", @@ -178,6 +173,71 @@ "Hymenoptera-2.5Kv2", "Anthozoa-1.7Kv1" ] + }, + "target_capture_mode": { + "type": "string", + "default": "illumina", + "description": "Simulate 'illumina' or 'pacbio' reads.", + "enum": [ + "illumina", + "pacbio" + ] + }, + "target_capture_fmedian": { + "type": "integer", + "default": 2000, + "description": "Median of fragment size at shearing." + }, + "target_capture_fshape": { + "type": "number", + "default": 6.0, + "description": "Shape parameter of the fragment size distribution." + }, + "target_capture_smedian": { + "type": "integer", + "default": 1300, + "description": "Median of fragment size distribution." + }, + "target_capture_sshape": { + "type": "number", + "default": 6.0, + "description": "Shape parameter of the fragment size distribution." + }, + "target_capture_tmedian": { + "type": "integer", + "default": null, + "description": "Median of target fragment size (the fragment size of the data). If specified, will override '--fmedian' and '--smedian'. Othersise will be estimated." + }, + "target_capture_tshape": { + "type": "number", + "default": null, + "description": "Shape parameter of the effective fragment size distribution." + }, + "target_capture_num": { + "type": "integer", + "default": 1000000, + "description": "Number of fragments." + }, + "target_capture_illen": { + "type": "integer", + "default": 300, + "description": "Illumina: read length." + }, + "target_capture_pblen": { + "type": "integer", + "default": 30000, + "description": "PacBio: Average (polymerase) read length." + }, + "target_capture_ilmode": { + "type": "string", + "default": "pe", + "description": "Illumina: Sequencing mode.", + "help_text": "'pe' = paired-end, 'mp' = mate-paired and 'se' = singled-end", + "enum": [ + "pe", + "mp", + "se" + ] } } }, diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index e64af1f..448484b 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -5,8 +5,7 @@ include { BOWTIE2_BUILD } from '../../modules/nf-core/bowtie2/build/main' include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/main' include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' -include { JAPSA_CAPSIM as CAPSIM_ILLUMINA } from '../../modules/local/japsa_capsim' -include { JAPSA_CAPSIM as CAPSIM_PACBIO } from '../../modules/local/japsa_capsim' +include { JAPSA_CAPSIM } from '../../modules/local/japsa_capsim' include { UNZIP as UNZIP_PROBE } from '../../modules/local/unzip' workflow TARGET_CAPTURE_WORKFLOW { @@ -80,41 +79,22 @@ workflow TARGET_CAPTURE_WORKFLOW { } // - // MODULE: Simulate target capture illumina reads + // MODULE: Simulate target capture reads // - ch_illumina_reads = Channel.empty() - CAPSIM_ILLUMINA ( + ch_reads = Channel.empty() + JAPSA_CAPSIM ( ch_capsim_input ) - ch_versions = ch_versions.mix(CAPSIM_ILLUMINA.out.versions.first()) - ch_illumina_reads = CAPSIM_ILLUMINA.out.fastq + ch_versions = ch_versions.mix(JAPSA_CAPSIM.out.versions.first()) + ch_reads = JAPSA_CAPSIM.out.fastq .map { meta, fastqs -> - meta.outdir = "capsim_illumina" - meta.datatype = "target_capture_illumina" + meta.outdir = "capsim" + meta.datatype = "target_capture" return [ meta, fastqs ] } - // - // MODULE: Simulate target capture pacbio reads - // - ch_pacbio_reads = Channel.empty() - if ( params.target_capture_pacbio ) { - CAPSIM_PACBIO ( - ch_capsim_input - ) - ch_versions = ch_versions.mix(CAPSIM_PACBIO.out.versions.first()) - ch_pacbio_reads = CAPSIM_PACBIO.out.fastq - .map { - meta, fastqs -> - meta.outdir = "capsim_pacbio" - meta.datatype = "target_capture_pacbio" - return [ meta, fastqs ] - } - } - emit: - illumina_reads = ch_illumina_reads // channel: [ meta, fastq ] - pacbio_reads = ch_pacbio_reads // channel: [ meta, fastq ] - versions = ch_versions // channel: [ versions.yml ] + reads = ch_reads // channel: [ meta, fastq ] + versions = ch_versions // channel: [ versions.yml ] } diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 4d74ec2..133d6d4 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -108,8 +108,7 @@ workflow READSIMULATOR { ch_probes.ifEmpty([]) ) ch_versions = ch_versions.mix(TARGET_CAPTURE_WORKFLOW.out.versions.first()) - ch_simulated_reads = ch_simulated_reads.mix(TARGET_CAPTURE_WORKFLOW.out.illumina_reads) - ch_simulated_reads = ch_simulated_reads.mix(TARGET_CAPTURE_WORKFLOW.out.pacbio_reads) + ch_simulated_reads = ch_simulated_reads.mix(TARGET_CAPTURE_WORKFLOW.out.reads) } // @@ -117,16 +116,15 @@ workflow READSIMULATOR { // if ( params.metagenome ) { INSILICOSEQ_GENERATE ( - ch_fasta.ifEmpty([]).first(), - ch_input + ch_input.combine(ch_fasta.ifEmpty([[]])) ) ch_versions = ch_versions.mix(INSILICOSEQ_GENERATE.out.versions.first()) ch_metagenome_reads = INSILICOSEQ_GENERATE.out.fastq .map { meta, fastqs -> - meta[0].outdir = "insilicoseq" - meta[0].datatype = "metagenomic_illumina" - return [ meta[0], fastqs ] + meta.outdir = "insilicoseq" + meta.datatype = "metagenomic_illumina" + return [ meta, fastqs ] } ch_simulated_reads = ch_simulated_reads.mix(ch_metagenome_reads) } @@ -136,16 +134,15 @@ workflow READSIMULATOR { // if ( params.wholegenome ) { WGSIM ( - ch_fasta.first(), - ch_input + ch_input.combine(ch_fasta) ) ch_versions = ch_versions.mix(WGSIM.out.versions.first()) ch_wholegenome_reads = WGSIM.out.fastq .map { meta, fastqs -> - meta[0].outdir = "wgsim" - meta[0].datatype = "wholegenome" - return [ meta[0], fastqs ] + meta.outdir = "wgsim" + meta.datatype = "wholegenome" + return [ meta, fastqs ] } ch_simulated_reads = ch_simulated_reads.mix(ch_wholegenome_reads) } From 96f38ea8f19491ca8ad9699942b223fc28195cf8 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Tue, 21 Nov 2023 04:18:56 +0000 Subject: [PATCH 47/89] Addressed lint error --- nextflow_schema.json | 13 ++----------- 1 file changed, 2 insertions(+), 11 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 13bc6f7..f0cffee 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -178,10 +178,7 @@ "type": "string", "default": "illumina", "description": "Simulate 'illumina' or 'pacbio' reads.", - "enum": [ - "illumina", - "pacbio" - ] + "enum": ["illumina", "pacbio"] }, "target_capture_fmedian": { "type": "integer", @@ -205,12 +202,10 @@ }, "target_capture_tmedian": { "type": "integer", - "default": null, "description": "Median of target fragment size (the fragment size of the data). If specified, will override '--fmedian' and '--smedian'. Othersise will be estimated." }, "target_capture_tshape": { "type": "number", - "default": null, "description": "Shape parameter of the effective fragment size distribution." }, "target_capture_num": { @@ -233,11 +228,7 @@ "default": "pe", "description": "Illumina: Sequencing mode.", "help_text": "'pe' = paired-end, 'mp' = mate-paired and 'se' = singled-end", - "enum": [ - "pe", - "mp", - "se" - ] + "enum": ["pe", "mp", "se"] } } }, From 928815afa889526c8600e48791898e0811b8daab Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 23 Nov 2023 00:02:05 +0000 Subject: [PATCH 48/89] Added svg file and added background to workflow diagram --- docs/images/readsimulator_workflow.png | Bin 90010 -> 98778 bytes docs/images/readsimulator_workflow.svg | 4 ++++ 2 files changed, 4 insertions(+) create mode 100755 docs/images/readsimulator_workflow.svg diff --git a/docs/images/readsimulator_workflow.png b/docs/images/readsimulator_workflow.png index 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    nf-core/readsimulator workflow overview @@ -20,7 +20,7 @@ ### Amplicon simulation steps -1. Create Reference database for Amplicon-Based Sequencing ([`CRABS`](https://github.com/gjeunen/reference_database_creator)) +1. Create reference database for amplicon-based sequencing ([`CRABS`](https://github.com/gjeunen/reference_database_creator)) 2. Simulate amplicon Illumina reads ([`art_illumina`](https://manpages.debian.org/testing/art-nextgen-simulation-tools/art_illumina.1.en.html)) 3. Create samplesheet with sample names and paths to simulated read files 4. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) diff --git a/modules/local/crabs/dbdownload/main.nf b/modules/local/crabs/dbdownload/main.nf index f3a9331..6db93f0 100644 --- a/modules/local/crabs/dbdownload/main.nf +++ b/modules/local/crabs/dbdownload/main.nf @@ -10,6 +10,9 @@ process CRABS_DBDOWNLOAD { 'biocontainers/crabs:0.1.1--pyhb7b1952_0' }" input: + val amplicon_ncbi_db + val amplicon_embl_db + val amplicon_bold_db output: path("*.fasta") , emit: fasta @@ -20,10 +23,17 @@ process CRABS_DBDOWNLOAD { script: def args = task.ext.args ?: '' + def ncbi_input = amplicon_ncbi_db ? "--source ncbi --database ${amplicon_ncbi_db} --output ncbi.fasta" : "" + def embl_input = amplicon_embl_db ? "--source embl --database ${params.amplicon_embl_db} --output embl.fasta " : "" + def bold_input = amplicon_bold_db ? "--source bold --database '${params.amplicon_bold_db} --output bold.fasta" : "" + // Check for clashing inputs here + + def input_args = ncbi_input + embl_input + bold_input def VERSION = '0.1.1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. """ crabs db_download \\ - $args + $args \\ + $input_args cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index c3d1c0d..8dbd736 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -75,7 +75,7 @@ workflow TARGET_CAPTURE_WORKFLOW { .combine ( SAMTOOLS_INDEX.out.bai.map { it = it[1] } ) .combine ( ch_input ) .map { - it = [ it[3], it[0], it[1], it[2] ] + fasta, bam, index, meta -> [ meta, fasta, bam, index ] } // From 1ac54cd8f3c685106c9482f30260ddcb0e9bd0c9 Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Thu, 30 Nov 2023 23:45:50 -0500 Subject: [PATCH 52/89] Apply suggestions from code review MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- README.md | 3 ++- assets/schema_input.json | 1 + docs/output.md | 6 +++--- docs/usage.md | 2 +- 4 files changed, 7 insertions(+), 5 deletions(-) diff --git a/README.md b/README.md index 51d2439..c7a3047 100644 --- a/README.md +++ b/README.md @@ -30,7 +30,7 @@ 1. Align probes to genome ([`Bowtie2`](https://bowtie-bio.sourceforge.net/bowtie2/index.shtml)) 2. Get SAM index ([`SAMtools`](https://www.htslib.org/)) -3. Simulate target capture reads (Illumina or Pacbio; default=Illumina) ([`Japsa capsim`](https://japsa.readthedocs.io/en/latest/tools/jsa.sim.capsim.html)) +3. Simulate target capture reads (Illumina (default) or Pacbio) ([`Japsa capsim`](https://japsa.readthedocs.io/en/latest/tools/jsa.sim.capsim.html)) 4. Create samplesheet with sample names and paths to simulated read files 5. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 6. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) @@ -63,6 +63,7 @@ First, prepare a samplesheet with your input data that looks as follows: ```csv sample,seed sample_1,1 +sample_2,4 ``` Each row represents an output sample. diff --git a/assets/schema_input.json b/assets/schema_input.json index 90d0551..bb76772 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -15,6 +15,7 @@ "meta": ["id"] }, "seed": { + "type": "integer", "errorMessage": "Seed must be provided", "unique": true, "meta": ["seed"] diff --git a/docs/output.md b/docs/output.md index 66f8adb..a9f0e49 100644 --- a/docs/output.md +++ b/docs/output.md @@ -61,12 +61,12 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - `*_1.fastq.gz`: Read 1 files simulating Illumina reads. The prefix will be the sample name found in the samplesheet. - `*_2.fastq.gz`: Read 2 files simulating Illumina reads. The prefix will be the sample name found in the samplesheet. - `capsim_pacbio/` - - `*_1.fastq.gz`: Read 1 files simulating Pacbio reads. The prefix will be the sample name found in the samplesheet. - - `*_1.fastq.gz`: Read 2 files simulating Pacbio reads. The prefix will be the sample name found in the samplesheet. + - `*_1.fastq.gz`: Read 1 files simulating PacBio reads. The prefix will be the sample name found in the samplesheet. + - `*_1.fastq.gz`: Read 2 files simulating PacBio reads. The prefix will be the sample name found in the samplesheet. -[CapSim](https://academic.oup.com/bioinformatics/article/34/5/873/4575140) is a tool to simulate capture sequencing reads. It's part of the Japsa package. For further reading and documentation see the [CapSim documentation](https://japsa.readthedocs.io/en/latest/tools/jsa.sim.capsim.html). +[CapSim](https://academic.oup.com/bioinformatics/article/34/5/873/4575140) is a tool to simulate capture sequencing reads. It's part of the [Japsa package](https://japsa.readthedocs.io/en/latest/). For further reading and documentation see the [CapSim documentation](https://japsa.readthedocs.io/en/latest/tools/jsa.sim.capsim.html). ### CRABS diff --git a/docs/usage.md b/docs/usage.md index f7345bc..4836a93 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -22,7 +22,7 @@ The samplesheet can have as many columns as you desire, however, there is a stri A final samplesheet file may look something like the one below. This is for 6 samples. -```console +```csv sample,seed sample_1,1 sample_2,2 From 66b4131f2a193fad0409c0441039a0ae416c7e73 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 19 Dec 2023 19:46:57 +0000 Subject: [PATCH 53/89] Template update for nf-core/tools version 2.11 --- .devcontainer/devcontainer.json | 1 + .github/CONTRIBUTING.md | 7 +- .github/PULL_REQUEST_TEMPLATE.md | 1 + .github/workflows/ci.yml | 2 +- .github/workflows/fix-linting.yml | 4 +- .github/workflows/linting.yml | 14 +- .github/workflows/release-announcments.yml | 68 +++++++++ .gitpod.yml | 4 +- CHANGELOG.md | 2 +- CITATIONS.md | 2 +- CODE_OF_CONDUCT.md | 133 ++++++++++++++---- README.md | 16 +-- assets/multiqc_config.yml | 6 +- assets/slackreport.json | 2 +- conf/modules.config | 9 ++ docs/output.md | 5 +- docs/usage.md | 20 ++- lib/NfcoreTemplate.groovy | 34 ++++- lib/WorkflowReadsimulator.groovy | 2 +- main.nf | 3 + modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 7 + .../custom/dumpsoftwareversions/main.nf | 6 +- .../custom/dumpsoftwareversions/meta.yml | 7 +- .../dumpsoftwareversions/tests/main.nf.test | 38 +++++ .../tests/main.nf.test.snap | 27 ++++ .../dumpsoftwareversions/tests/tags.yml | 2 + modules/nf-core/fastqc/environment.yml | 7 + modules/nf-core/fastqc/main.nf | 16 ++- modules/nf-core/fastqc/meta.yml | 5 + modules/nf-core/fastqc/tests/main.nf.test | 109 ++++++++++++++ .../nf-core/fastqc/tests/main.nf.test.snap | 10 ++ modules/nf-core/fastqc/tests/tags.yml | 2 + modules/nf-core/multiqc/environment.yml | 7 + modules/nf-core/multiqc/main.nf | 8 +- modules/nf-core/multiqc/meta.yml | 11 +- modules/nf-core/multiqc/tests/main.nf.test | 63 +++++++++ modules/nf-core/multiqc/tests/tags.yml | 2 + nextflow.config | 19 ++- nextflow_schema.json | 15 -- workflows/readsimulator.nf | 1 + 41 files changed, 587 insertions(+), 116 deletions(-) create mode 100644 .github/workflows/release-announcments.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap create mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml create mode 100644 modules/nf-core/fastqc/environment.yml create mode 100644 modules/nf-core/fastqc/tests/main.nf.test create mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap create mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 modules/nf-core/multiqc/environment.yml create mode 100644 modules/nf-core/multiqc/tests/main.nf.test create mode 100644 modules/nf-core/multiqc/tests/tags.yml diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index ea27a58..4ecfbfe 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -2,6 +2,7 @@ "name": "nfcore", "image": "nfcore/gitpod:latest", "remoteUser": "gitpod", + "runArgs": ["--privileged"], // Configure tool-specific properties. "customizations": { diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 6d9461b..25e2fd5 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,7 +9,9 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -> If you need help using or modifying nf-core/readsimulator then the best place to ask is on the nf-core Slack [#readsimulator](https://nfcore.slack.com/channels/readsimulator) channel ([join our Slack here](https://nf-co.re/join/slack)). +:::info +If you need help using or modifying nf-core/readsimulator then the best place to ask is on the nf-core Slack [#readsimulator](https://nfcore.slack.com/channels/readsimulator) channel ([join our Slack here](https://nf-co.re/join/slack)). +::: ## Contribution workflow @@ -25,6 +27,9 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests +You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to +receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. + When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 3ce6b23..440b520 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -19,6 +19,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/read - [ ] If necessary, also make a PR on the nf-core/readsimulator _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. - [ ] `CHANGELOG.md` is updated. diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 9d9993b..3590023 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,7 +28,7 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 4c48270..4d6d495 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -13,7 +13,7 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 with: token: ${{ secrets.nf_core_bot_auth_token }} @@ -24,7 +24,7 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install Prettier run: npm install -g prettier @prettier/plugin-php diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 888cb4b..905c58e 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,9 +14,9 @@ jobs: EditorConfig: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install editorconfig-checker run: npm install -g editorconfig-checker @@ -27,9 +27,9 @@ jobs: Prettier: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - - uses: actions/setup-node@v3 + - uses: actions/setup-node@v4 - name: Install Prettier run: npm install -g prettier @@ -40,7 +40,7 @@ jobs: PythonBlack: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v3 + - uses: actions/checkout@v4 - name: Check code lints with Black uses: psf/black@stable @@ -71,14 +71,14 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v3 + uses: actions/checkout@v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@v4 with: - python-version: "3.8" + python-version: "3.11" architecture: "x64" - name: Install dependencies diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcments.yml new file mode 100644 index 0000000..6ad3392 --- /dev/null +++ b/.github/workflows/release-announcments.yml @@ -0,0 +1,68 @@ +name: release-announcements +# Automatic release toot and tweet anouncements +on: + release: + types: [published] + workflow_dispatch: + +jobs: + toot: + runs-on: ubuntu-latest + steps: + - uses: rzr/fediverse-action@master + with: + access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} + host: "mstdn.science" # custom host if not "mastodon.social" (default) + # GitHub event payload + # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release + message: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + + send-tweet: + runs-on: ubuntu-latest + + steps: + - uses: actions/setup-python@v4 + with: + python-version: "3.10" + - name: Install dependencies + run: pip install tweepy==4.14.0 + - name: Send tweet + shell: python + run: | + import os + import tweepy + + client = tweepy.Client( + access_token=os.getenv("TWITTER_ACCESS_TOKEN"), + access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"), + consumer_key=os.getenv("TWITTER_CONSUMER_KEY"), + consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"), + ) + tweet = os.getenv("TWEET") + client.create_tweet(text=tweet) + env: + TWEET: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }} + TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }} + TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }} + TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }} + + bsky-post: + runs-on: ubuntu-latest + steps: + - uses: zentered/bluesky-post-action@v0.0.2 + with: + post: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + env: + BSKY_IDENTIFIER: ${{ secrets.BSKY_IDENTIFIER }} + BSKY_PASSWORD: ${{ secrets.BSKY_PASSWORD }} + # diff --git a/.gitpod.yml b/.gitpod.yml index 25488dc..acf7269 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -4,7 +4,9 @@ tasks: command: | pre-commit install --install-hooks nextflow self-update - + - name: unset JAVA_TOOL_OPTIONS + command: | + unset JAVA_TOOL_OPTIONS vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/CHANGELOG.md b/CHANGELOG.md index ec9fbd7..7fdc7e3 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v1.0dev - [date] +## v1.0.0 - [date] Initial release of nf-core/readsimulator, created with the [nf-core](https://nf-co.re/) template. diff --git a/CITATIONS.md b/CITATIONS.md index 6b7717f..c2f8281 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -12,7 +12,7 @@ - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) - > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. Available online https://www.bioinformatics.babraham.ac.uk/projects/fastqc/. + > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index f4fd052..c089ec7 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -1,18 +1,20 @@ -# Code of Conduct at nf-core (v1.0) +# Code of Conduct at nf-core (v1.4) ## Our Pledge -In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: - Age +- Ability - Body size +- Caste - Familial status - Gender identity and expression - Geographical location - Level of experience - Nationality and national origins - Native language -- Physical and neurological ability +- Neurodiversity - Race or ethnicity - Religion - Sexual identity and orientation @@ -22,80 +24,133 @@ Please note that the list above is alphabetised and is therefore not ranked in a ## Preamble -> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. +:::note +This Code of Conduct (CoC) has been drafted by Renuka Kudva, Cris Tuñí, and Michael Heuer, with input from the nf-core Core Team and Susanna Marquez from the nf-core community. "We", in this document, refers to the Safety Officers and members of the nf-core Core Team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will be amended periodically to keep it up-to-date. In case of any dispute, the most current version will apply. +::: -An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). + +Our Safety Officers are Saba Nafees, Cris Tuñí, and Michael Heuer. nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. -We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. +We have therefore adopted this CoC, which we require all members of our community and attendees of nf-core events to adhere to in all our workspaces at all times. Workspaces include, but are not limited to, Slack, meetings on Zoom, gather.town, YouTube live etc. -Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. +Our CoC will be strictly enforced and the nf-core team reserves the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. -We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. +We ask all members of our community to help maintain supportive and productive workspaces and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. -Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re +Questions, concerns, or ideas on what we can include? Contact members of the Safety Team on Slack or email safety [at] nf-co [dot] re. ## Our Responsibilities -The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. +Members of the Safety Team (the Safety Officers) are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. -The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. +The Safety Team, in consultation with the nf-core core team, have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this CoC, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. -Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. +Members of the core team or the Safety Team who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and will be subject to the same actions as others in violation of the CoC. -## When are where does this Code of Conduct apply? +## When and where does this Code of Conduct apply? -Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events, such as hackathons, workshops, bytesize, and collaborative workspaces on gather.town. These guidelines include, but are not limited to, the following (listed alphabetically and therefore in no order of preference): - Communicating with an official project email address. - Communicating with community members within the nf-core Slack channel. - Participating in hackathons organised by nf-core (both online and in-person events). -- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. -- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence, and on the nf-core gather.town workspace. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, gather.town, Jitsi, YouTube live etc. - Representing nf-core on social media. This includes both official and personal accounts. ## nf-core cares 😊 -nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include, but are not limited to, the following (listed in alphabetical order): - Ask for consent before sharing another community member’s personal information (including photographs) on social media. - Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. -- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Celebrate your accomplishments! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) - Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) - Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) - Focus on what is best for the team and the community. (When in doubt, ask) -- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. +- Accept feedback, yet be unafraid to question, deliberate, and learn. - Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) -- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communication to be kind.**) - Take breaks when you feel like you need them. -- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) +- Use welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack) ## nf-core frowns on 😕 -The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this CoC. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces: - Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. - “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. - Spamming or trolling of individuals on social media. -- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. -- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. +- Use of sexual or discriminatory imagery, comments, jokes, or unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion, or work experience. ### Online Trolling -The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the risk of online trolling. This is unacceptable — reports of such behaviour will be taken very seriously and perpetrators will be excluded from activities immediately. -All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. +All community members are **required** to ask members of the group they are working with for explicit consent prior to taking screenshots of individuals during video calls. -## Procedures for Reporting CoC violations +## Procedures for reporting CoC violations If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. -You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). +You can reach out to members of the Safety Team (Saba Nafees, Cris Tuñí, and Michael Heuer) on Slack. Alternatively, contact a member of the nf-core core team [nf-core core team](https://nf-co.re/about), and they will forward your concerns to the Safety Team. + +Issues directly concerning members of the Core Team or the Safety Team will be dealt with by other members of the core team and the safety manager — possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson and details will be shared in due course. + +All reports will be handled with the utmost discretion and confidentiality. + +You can also report any CoC violations to safety [at] nf-co [dot] re. In your email report, please do your best to include: + +- Your contact information. +- Identifying information (e.g. names, nicknames, pseudonyms) of the participant who has violated the Code of Conduct. +- The behaviour that was in violation and the circumstances surrounding the incident. +- The approximate time of the behaviour (if different than the time the report was made). +- Other people involved in the incident, if applicable. +- If you believe the incident is ongoing. +- If there is a publicly available record (e.g. mailing list record, a screenshot). +- Any additional information. + +After you file a report, one or more members of our Safety Team will contact you to follow up on your report. + +## Who will read and handle reports + +All reports will be read and handled by the members of the Safety Team at nf-core. + +If members of the Safety Team are deemed to have a conflict of interest with a report, they will be required to recuse themselves as per our Code of Conduct and will not have access to any follow-ups. + +To keep this first report confidential from any of the Safety Team members, please submit your first report by direct messaging on Slack/direct email to any of the nf-core members you are comfortable disclosing the information to, and be explicit about which member(s) you do not consent to sharing the information with. + +## Reviewing reports + +After receiving the report, members of the Safety Team will review the incident report to determine whether immediate action is required, for example, whether there is immediate threat to participants’ safety. + +The Safety Team, in consultation with members of the nf-core core team, will assess the information to determine whether the report constitutes a Code of Conduct violation, for them to decide on a course of action. + +In the case of insufficient information, one or more members of the Safety Team may contact the reporter, the reportee, or any other attendees to obtain more information. -Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. +Once additional information is gathered, the Safety Team will collectively review and decide on the best course of action to take, if any. The Safety Team reserves the right to not act on a report. -All reports will be handled with utmost discretion and confidentially. +## Confidentiality + +All reports, and any additional information included, are only shared with the team of safety officers (and possibly members of the core team, in case the safety officer is in violation of the CoC). We will respect confidentiality requests for the purpose of protecting victims of abuse. + +We will not name harassment victims, beyond discussions between the safety officer and members of the nf-core team, without the explicit consent of the individuals involved. + +## Enforcement + +Actions taken by the nf-core’s Safety Team may include, but are not limited to: + +- Asking anyone to stop a behaviour. +- Asking anyone to leave the event and online spaces either temporarily, for the remainder of the event, or permanently. +- Removing access to the gather.town and Slack, either temporarily or permanently. +- Communicating to all participants to reinforce our expectations for conduct and remind what is unacceptable behaviour; this may be public for practical reasons. +- Communicating to all participants that an incident has taken place and how we will act or have acted — this may be for the purpose of letting event participants know we are aware of and dealing with the incident. +- Banning anyone from participating in nf-core-managed spaces, future events, and activities, either temporarily or permanently. +- No action. ## Attribution and Acknowledgements @@ -106,6 +161,22 @@ All reports will be handled with utmost discretion and confidentially. ## Changelog -### v1.0 - March 12th, 2021 +### v1.4 - February 8th, 2022 + +- Included a new member of the Safety Team. Corrected a typographical error in the text. + +### v1.3 - December 10th, 2021 + +- Added a statement that the CoC applies to nf-core gather.town workspaces. Corrected typographical errors in the text. + +### v1.2 - November 12th, 2021 + +- Removed information specific to reporting CoC violations at the Hackathon in October 2021. + +### v1.1 - October 14th, 2021 + +- Updated with names of new Safety Officers and specific information for the hackathon in October 2021. + +### v1.0 - March 15th, 2021 - Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/README.md b/README.md index 8019e4d..77990b6 100644 --- a/README.md +++ b/README.md @@ -1,6 +1,7 @@ # ![nf-core/readsimulator](docs/images/nf-core-readsimulator_logo_light.png#gh-light-mode-only) ![nf-core/readsimulator](docs/images/nf-core-readsimulator_logo_dark.png#gh-dark-mode-only) -[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/readsimulator/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![GitHub Actions CI Status](https://github.com/nf-core/readsimulator/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/readsimulator/actions?query=workflow%3A%22nf-core+CI%22) +[![GitHub Actions Linting Status](https://github.com/nf-core/readsimulator/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/readsimulator/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/readsimulator/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) @@ -29,10 +30,8 @@ ## Usage -> **Note** -> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how -> to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) -> with `-profile test` before running the workflow on actual data. +> [!NOTE] +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. - + diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 28d3fd1..9491aa7 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,9 @@ report_comment: > - This report has been generated by the nf-core/readsimulator + + This report has been generated by the nf-core/readsimulator analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. + report_section_order: "nf-core-readsimulator-methods-description": order: -1000 diff --git a/assets/slackreport.json b/assets/slackreport.json index cfdb0eb..e9d5f8a 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "nf-core/readsimulator v${version} - ${runName}", + "author_name": "nf-core/readsimulator ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/conf/modules.config b/conf/modules.config index da58a5d..d91c6ab 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -38,4 +38,13 @@ process { ] } + withName: 'MULTIQC' { + ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } + publishDir = [ + path: { "${params.outdir}/multiqc" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + } diff --git a/docs/output.md b/docs/output.md index 31a4766..fa78237 100644 --- a/docs/output.md +++ b/docs/output.md @@ -35,7 +35,9 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d ![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) -> **NB:** The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. +:::note +The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. +::: ### MultiQC @@ -62,6 +64,7 @@ Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQ - Reports generated by Nextflow: `execution_report.html`, `execution_timeline.html`, `execution_trace.txt` and `pipeline_dag.dot`/`pipeline_dag.svg`. - Reports generated by the pipeline: `pipeline_report.html`, `pipeline_report.txt` and `software_versions.yml`. The `pipeline_report*` files will only be present if the `--email` / `--email_on_fail` parameter's are used when running the pipeline. - Reformatted samplesheet files used as input to the pipeline: `samplesheet.valid.csv`. + - Parameters used by the pipeline run: `params.json`. diff --git a/docs/usage.md b/docs/usage.md index 8596ff1..8c5db75 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -20,7 +20,7 @@ You will need to create a samplesheet with information about the samples you wou The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz @@ -33,7 +33,7 @@ The pipeline will auto-detect whether a sample is single- or paired-end using th A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz @@ -75,7 +75,9 @@ If you wish to repeatedly use the same parameters for multiple runs, rather than Pipeline settings can be provided in a `yaml` or `json` file via `-params-file `. -> ⚠️ Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). +:::warning +Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). +::: The above pipeline run specified with a params file in yaml format: @@ -112,11 +114,15 @@ This version number will be logged in reports when you run the pipeline, so that To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter. -> 💡 If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. +:::tip +If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. +::: ## Core Nextflow arguments -> **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). +:::note +These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). +::: ### `-profile` @@ -124,7 +130,9 @@ Use this parameter to choose a configuration profile. Profiles can give configur Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below. -> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. +:::info +We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. +::: The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 408951a..e248e4c 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -3,6 +3,8 @@ // import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx class NfcoreTemplate { @@ -140,12 +142,14 @@ class NfcoreTemplate { try { if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } [ 'sendmail', '-t' ].execute() << sendmail_html log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" } catch (all) { // Catch failures and try with plaintext def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { mail_cmd += [ '-A', mqc_report ] } mail_cmd.execute() << email_html @@ -154,14 +158,16 @@ class NfcoreTemplate { } // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() } // @@ -222,6 +228,20 @@ class NfcoreTemplate { } } + // + // Dump pipeline parameters in a json file + // + public static void dump_parameters(workflow, params) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() + } + // // Print pipeline summary on completion // diff --git a/lib/WorkflowReadsimulator.groovy b/lib/WorkflowReadsimulator.groovy index c7cc20d..9dd5e20 100755 --- a/lib/WorkflowReadsimulator.groovy +++ b/lib/WorkflowReadsimulator.groovy @@ -53,7 +53,7 @@ class WorkflowReadsimulator { public static String toolCitationText(params) { - // TODO Optionally add in-text citation tools to this list. + // TODO nf-core: Optionally add in-text citation tools to this list. // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", // Uncomment function in methodsDescriptionText to render in MultiQC report def citation_text = [ diff --git a/main.nf b/main.nf index f050787..e148bf9 100644 --- a/main.nf +++ b/main.nf @@ -17,6 +17,9 @@ nextflow.enable.dsl = 2 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ +// TODO nf-core: Remove this line if you don't need a FASTA file +// This is an example of how to use getGenomeAttribute() to fetch parameters +// from igenomes.config using `--genome` params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') /* diff --git a/modules.json b/modules.json index 039aea8..7ed6f2c 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "76cc4938c1f6ea5c7d83fed1eeffc146787f9543", + "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "f2d63bd5b68925f98f572eed70993d205cc694b7", + "git_sha": "4ab13872435962dadc239979554d13709e20bf29", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml new file mode 100644 index 0000000..f0c63f6 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -0,0 +1,7 @@ +name: custom_dumpsoftwareversions +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.17 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 800a609..7685b33 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : + 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index c32657d..5f15a5f 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,4 +1,4 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: @@ -16,7 +16,6 @@ input: type: file description: YML file containing software versions pattern: "*.yml" - output: - yml: type: file @@ -30,7 +29,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@drpatelh" - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test new file mode 100644 index 0000000..eec1db1 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -0,0 +1,38 @@ +nextflow_process { + + name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" + script "../main.nf" + process "CUSTOM_DUMPSOFTWAREVERSIONS" + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "dumpsoftwareversions" + tag "custom/dumpsoftwareversions" + + test("Should run without failures") { + when { + process { + """ + def tool1_version = ''' + TOOL1: + tool1: 0.11.9 + '''.stripIndent() + + def tool2_version = ''' + TOOL2: + tool2: 1.9 + '''.stripIndent() + + input[0] = Channel.of(tool1_version, tool2_version).collectFile() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap new file mode 100644 index 0000000..4274ed5 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -0,0 +1,27 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ], + "1": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "2": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "mqc_yml": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "versions": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "yml": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ] + } + ], + "timestamp": "2023-11-03T14:43:22.157011" + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml new file mode 100644 index 0000000..405aa24 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml @@ -0,0 +1,2 @@ +custom/dumpsoftwareversions: + - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml new file mode 100644 index 0000000..1787b38 --- /dev/null +++ b/modules/nf-core/fastqc/environment.yml @@ -0,0 +1,7 @@ +name: fastqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 9ae5838..9e19a74 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -2,10 +2,10 @@ process FASTQC { tag "$meta.id" label 'process_medium' - conda "bioconda::fastqc=0.11.9" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' : - 'quay.io/biocontainers/fastqc:0.11.9--0' }" + 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : + 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" input: tuple val(meta), path(reads) @@ -29,11 +29,15 @@ process FASTQC { printf "%s %s\\n" $rename_to | while read old_name new_name; do [ -f "\${new_name}" ] || ln -s \$old_name \$new_name done - fastqc $args --threads $task.cpus $renamed_files + + fastqc \\ + $args \\ + --threads $task.cpus \\ + $renamed_files cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ @@ -45,7 +49,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ } diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml index 4da5bb5..ee5507e 100644 --- a/modules/nf-core/fastqc/meta.yml +++ b/modules/nf-core/fastqc/meta.yml @@ -50,3 +50,8 @@ authors: - "@grst" - "@ewels" - "@FelixKrueger" +maintainers: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test new file mode 100644 index 0000000..b9e8f92 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -0,0 +1,109 @@ +nextflow_process { + + name "Test Process FASTQC" + script "../main.nf" + process "FASTQC" + tag "modules" + tag "modules_nfcore" + tag "fastqc" + + test("Single-Read") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id: 'test', single_end:true ], + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:

    Mon 2 Oct 2023
    test.gz
    + // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, + { assert path(process.out.html.get(0).get(1)).getText().contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + ) + } + } +// TODO +// // +// // Test with paired-end data +// // +// workflow test_fastqc_paired_end { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with interleaved data +// // +// workflow test_fastqc_interleaved { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with bam data +// // +// workflow test_fastqc_bam { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with multiple samples +// // +// workflow test_fastqc_multiple { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with custom prefix +// // +// workflow test_fastqc_custom_prefix { +// input = [ +// [ id:'mysample', single_end:true ], // meta map +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } +} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap new file mode 100644 index 0000000..636a32c --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -0,0 +1,10 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2023-10-09T23:40:54+0000" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml new file mode 100644 index 0000000..7834294 --- /dev/null +++ b/modules/nf-core/fastqc/tests/tags.yml @@ -0,0 +1,2 @@ +fastqc: + - modules/nf-core/fastqc/** diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml new file mode 100644 index 0000000..bc0bdb5 --- /dev/null +++ b/modules/nf-core/multiqc/environment.yml @@ -0,0 +1,7 @@ +name: multiqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.18 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 4b60474..00cc48d 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,10 +1,10 @@ process MULTIQC { label 'process_single' - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : + 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -25,12 +25,14 @@ process MULTIQC { def args = task.ext.args ?: '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' """ multiqc \\ --force \\ $args \\ $config \\ $extra_config \\ + $logo \\ . cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f93b5ee..f1aa660 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,5 +1,5 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json -name: MultiQC +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: - QC @@ -13,7 +13,6 @@ tools: homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ licence: ["GPL-3.0-or-later"] - input: - multiqc_files: type: file @@ -31,7 +30,6 @@ input: type: file description: Optional logo file for MultiQC pattern: "*.{png}" - output: - report: type: file @@ -54,3 +52,8 @@ authors: - "@bunop" - "@drpatelh" - "@jfy133" +maintainers: + - "@abhi18av" + - "@bunop" + - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test new file mode 100644 index 0000000..c2dad21 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -0,0 +1,63 @@ +nextflow_process { + + name "Test Process MULTIQC" + script "../main.nf" + process "MULTIQC" + tag "modules" + tag "modules_nfcore" + tag "multiqc" + + test("MULTIQC: FASTQC") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } + + test("MULTIQC: FASTQC and a config file") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } +} diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml new file mode 100644 index 0000000..bea6c0d --- /dev/null +++ b/modules/nf-core/multiqc/tests/tags.yml @@ -0,0 +1,2 @@ +multiqc: + - modules/nf-core/multiqc/** diff --git a/nextflow.config b/nextflow.config index 8f89683..5ae73fd 100644 --- a/nextflow.config +++ b/nextflow.config @@ -14,7 +14,7 @@ params { input = null // References genome = null - igenomes_base = 's3://ngi-igenomes/igenomes' + igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false @@ -54,7 +54,7 @@ params { // Schema validation default options validationFailUnrecognisedParams = false validationLenientMode = false - validationSchemaIgnoreParams = 'genomes' + validationSchemaIgnoreParams = 'genomes,igenomes_base' validationShowHiddenParams = false validate_params = true @@ -82,6 +82,7 @@ profiles { dumpHashes = true process.beforeScript = 'echo $HOSTNAME' cleanup = false + nextflow.enable.configProcessNamesValidation = true } conda { conda.enabled = true @@ -104,13 +105,13 @@ profiles { } docker { docker.enabled = true - docker.userEmulation = true conda.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false + runOptions = '-u $(id -u):$(id -g)' } arm { docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' @@ -154,6 +155,7 @@ profiles { } apptainer { apptainer.enabled = true + apptainer.autoMounts = true conda.enabled = false docker.enabled = false singularity.enabled = false @@ -163,8 +165,8 @@ profiles { } gitpod { executor.name = 'local' - executor.cpus = 16 - executor.memory = 60.GB + executor.cpus = 4 + executor.memory = 8.GB } test { includeConfig 'conf/test.config' } test_full { includeConfig 'conf/test_full.config' } @@ -180,7 +182,7 @@ singularity.registry = 'quay.io' // Nextflow plugins plugins { - id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet } // Load igenomes.config if required @@ -203,6 +205,9 @@ env { // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Disable process selector warnings by default. Use debug profile to enable warnings. +nextflow.enable.configProcessNamesValidation = false + def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true @@ -228,7 +233,7 @@ manifest { description = """A workflow to simulate reads""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '1.0dev' + version = '1.0.0' doi = '' } diff --git a/nextflow_schema.json b/nextflow_schema.json index e2112bb..4059444 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -64,14 +64,6 @@ "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", "fa_icon": "far fa-file-code" }, - "igenomes_base": { - "type": "string", - "format": "directory-path", - "description": "Directory / URL base for iGenomes references.", - "default": "s3://ngi-igenomes/igenomes", - "fa_icon": "fas fa-cloud-download-alt", - "hidden": true - }, "igenomes_ignore": { "type": "boolean", "description": "Do not load the iGenomes reference config.", @@ -175,14 +167,12 @@ "type": "boolean", "description": "Display help text.", "fa_icon": "fas fa-question-circle", - "default": false, "hidden": true }, "version": { "type": "boolean", "description": "Display version and exit.", "fa_icon": "fas fa-question-circle", - "default": false, "hidden": true }, "publish_dir_mode": { @@ -206,7 +196,6 @@ "type": "boolean", "description": "Send plain-text email instead of HTML.", "fa_icon": "fas fa-remove-format", - "default": false, "hidden": true }, "max_multiqc_email_size": { @@ -221,7 +210,6 @@ "type": "boolean", "description": "Do not use coloured log outputs.", "fa_icon": "fas fa-palette", - "default": false, "hidden": true }, "hook_url": { @@ -260,7 +248,6 @@ "type": "boolean", "fa_icon": "far fa-eye-slash", "description": "Show all params when using `--help`", - "default": false, "hidden": true, "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." }, @@ -268,7 +255,6 @@ "type": "boolean", "fa_icon": "far fa-check-circle", "description": "Validation of parameters fails when an unrecognised parameter is found.", - "default": false, "hidden": true, "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." }, @@ -276,7 +262,6 @@ "type": "boolean", "fa_icon": "far fa-check-circle", "description": "Validation of parameters in lenient more.", - "default": false, "hidden": true, "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." } diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 8a2b8b3..44c3497 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -120,6 +120,7 @@ workflow.onComplete { if (params.email || params.email_on_fail) { NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) } + NfcoreTemplate.dump_parameters(workflow, params) NfcoreTemplate.summary(workflow, params, log) if (params.hook_url) { NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) From 00986602029c57b8dd9c5e45eee181959d1abaf0 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 20 Dec 2023 17:05:00 +0000 Subject: [PATCH 54/89] Template update for nf-core/tools version 2.11.1 --- .../{release-announcments.yml => release-announcements.yml} | 0 nextflow.config | 4 ++-- 2 files changed, 2 insertions(+), 2 deletions(-) rename .github/workflows/{release-announcments.yml => release-announcements.yml} (100%) diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcements.yml similarity index 100% rename from .github/workflows/release-announcments.yml rename to .github/workflows/release-announcements.yml diff --git a/nextflow.config b/nextflow.config index 5ae73fd..a6a8494 100644 --- a/nextflow.config +++ b/nextflow.config @@ -111,10 +111,10 @@ profiles { shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - runOptions = '-u $(id -u):$(id -g)' + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { singularity.enabled = true From fcccb1b2706f62c15e0a2d69229b9f19c0015632 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Tue, 9 Jan 2024 08:22:17 +0800 Subject: [PATCH 55/89] Update main.nf --- modules/local/crabs/dbdownload/main.nf | 2 -- 1 file changed, 2 deletions(-) diff --git a/modules/local/crabs/dbdownload/main.nf b/modules/local/crabs/dbdownload/main.nf index 6db93f0..acfb7cf 100644 --- a/modules/local/crabs/dbdownload/main.nf +++ b/modules/local/crabs/dbdownload/main.nf @@ -26,8 +26,6 @@ process CRABS_DBDOWNLOAD { def ncbi_input = amplicon_ncbi_db ? "--source ncbi --database ${amplicon_ncbi_db} --output ncbi.fasta" : "" def embl_input = amplicon_embl_db ? "--source embl --database ${params.amplicon_embl_db} --output embl.fasta " : "" def bold_input = amplicon_bold_db ? "--source bold --database '${params.amplicon_bold_db} --output bold.fasta" : "" - // Check for clashing inputs here - def input_args = ncbi_input + embl_input + bold_input def VERSION = '0.1.1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. """ From 96ee54bdfc1f22e204d3e71be6f4e135486e5d60 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Tue, 9 Jan 2024 08:28:29 +0800 Subject: [PATCH 56/89] Update schema_input.json --- assets/schema_input.json | 48 ++++++++++++++++++++-------------------- 1 file changed, 24 insertions(+), 24 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index bb76772..2a6c7ba 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,26 +1,26 @@ { - "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/readsimulator/master/assets/schema_input.json", - "title": "nf-core/readsimulator pipeline - params.input schema", - "description": "Schema for the file provided with params.input", - "type": "array", - "items": { - "type": "object", - "properties": { - "sample": { - "type": "string", - "pattern": "^\\S+$", - "unique": true, - "errorMessage": "Sample name must be provided and cannot contain spaces", - "meta": ["id"] - }, - "seed": { - "type": "integer", - "errorMessage": "Seed must be provided", - "unique": true, - "meta": ["seed"] - } - }, - "required": ["sample", "seed"] - } + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/readsimulator/master/assets/schema_input.json", + "title": "nf-core/readsimulator pipeline - params.input schema", + "description": "Schema for the file provided with params.input", + "type": "array", + "items": { + "type": "object", + "properties": { + "sample": { + "type": "string", + "pattern": "^\\S+$", + "unique": true, + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] + }, + "seed": { + "type": "integer", + "errorMessage": "Seed must be provided", + "unique": true, + "meta": ["seed"] + } + }, + "required": ["sample", "seed"] + } } From 2f4336282d3f16c9ec0d1166daa7ca9c7a45ec84 Mon Sep 17 00:00:00 2001 From: Adam Bennett <43841526+a4000@users.noreply.github.com> Date: Tue, 9 Jan 2024 08:58:49 +0800 Subject: [PATCH 57/89] Update schema_input.json From 30f6674a6850e6e0a6c988511402ec82812e56b3 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Tue, 9 Jan 2024 01:03:16 +0000 Subject: [PATCH 58/89] updated assets/schema_input.json --- assets/schema_input.json | 48 ++++++++++++++++++++-------------------- 1 file changed, 24 insertions(+), 24 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index 2a6c7ba..c50c66d 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,26 +1,26 @@ { - "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/readsimulator/master/assets/schema_input.json", - "title": "nf-core/readsimulator pipeline - params.input schema", - "description": "Schema for the file provided with params.input", - "type": "array", - "items": { - "type": "object", - "properties": { - "sample": { - "type": "string", - "pattern": "^\\S+$", - "unique": true, - "errorMessage": "Sample name must be provided and cannot contain spaces", - "meta": ["id"] - }, - "seed": { - "type": "integer", - "errorMessage": "Seed must be provided", - "unique": true, - "meta": ["seed"] - } - }, - "required": ["sample", "seed"] - } + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/readsimulator/master/assets/schema_input.json", + "title": "nf-core/readsimulator pipeline - params.input schema", + "description": "Schema for the file provided with params.input", + "type": "array", + "items": { + "type": "object", + "properties": { + "sample": { + "type": "string", + "pattern": "^\\S+$", + "unique": true, + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] + }, + "seed": { + "type": "integer", + "errorMessage": "Seed must be provided", + "unique": true, + "meta": ["seed"] + } + }, + "required": ["sample", "seed"] + } } From 53287641c292299b9e34c0c67069d7ae2803e3f8 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Tue, 9 Jan 2024 02:06:50 +0000 Subject: [PATCH 59/89] Made modifications based on PR comments --- README.md | 8 ++++---- assets/schema_input.json | 2 +- docs/images/readsimulator_workflow.png | Bin 98778 -> 140021 bytes docs/images/readsimulator_workflow.svg | 2 +- docs/output.md | 14 +++++++------- modules/local/crabs/dbdownload/main.nf | 13 ++++++------- modules/local/wgsim/main.nf | 11 ++++------- nextflow.config | 8 ++++---- nextflow_schema.json | 14 +++++++++----- subworkflows/local/amplicon_workflow.nf | 6 +++++- workflows/readsimulator.nf | 4 +++- 11 files changed, 44 insertions(+), 38 deletions(-) diff --git a/README.md b/README.md index c7a3047..72495b3 100644 --- a/README.md +++ b/README.md @@ -22,7 +22,7 @@ 1. Create reference database for amplicon-based sequencing ([`CRABS`](https://github.com/gjeunen/reference_database_creator)) 2. Simulate amplicon Illumina reads ([`art_illumina`](https://manpages.debian.org/testing/art-nextgen-simulation-tools/art_illumina.1.en.html)) -3. Create samplesheet with sample names and paths to simulated read files +3. Create samplesheet with sample names and paths to simulated read files (header = sample,fastq_1,fastq_2) 4. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 5. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) @@ -31,21 +31,21 @@ 1. Align probes to genome ([`Bowtie2`](https://bowtie-bio.sourceforge.net/bowtie2/index.shtml)) 2. Get SAM index ([`SAMtools`](https://www.htslib.org/)) 3. Simulate target capture reads (Illumina (default) or Pacbio) ([`Japsa capsim`](https://japsa.readthedocs.io/en/latest/tools/jsa.sim.capsim.html)) -4. Create samplesheet with sample names and paths to simulated read files +4. Create samplesheet with sample names and paths to simulated read files (header = sample,fastq_1,fastq_2) 5. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 6. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) ### Metagenome simulation steps 1. Simulate metagenome Illumina reads ([`InsilicoSeq Generate`](https://insilicoseq.readthedocs.io/en/latest/)) -2. Create samplesheet with sample names and paths to simulated read files +2. Create samplesheet with sample names and paths to simulated read files (header = sample,fastq_1,fastq_2) 3. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 4. Present QC for simulated reads ([`MultiQC`](http://multiqc.info/)) ### Wholegenome simulation steps 1. Simulate wholegenome reads ([`wgsim`](https://github.com/lh3/wgsim)) -2. Create samplesheet with sample names and paths to simulated read files +2. Create samplesheet with sample names and paths to simulated read files (header = sample,fastq_1,fastq_2) 3. Simulated read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 4. 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zI&m1fkPI-`GxkCcR|8tFlV=?t$%8o-jwa?cpnyGKwN?(=##|8TLs<>gNmT04p+DSu z7f6ix0@6~Ky5zmr3MOPAtAE;%wa=>38BTk05pxALH>r7gYq;1$GtSyjOt;wknt_=PaNMpOsW4qKT zHej16oM;d3;ej)tfRb-N2`}&fvuyu|-^Lk8K}o2IjDE)U`~6(+~q%1fxL(iLK&D_Tzuv-L+o_6#1?2TxE)gO^V4Te^LwiD6MDa- zwj0WS`q|NFBAf#Y6VrPDT7A=}-S2=@BnOYbZZwl;`*%SmI#;bXBr_E0jPP8 AQ~&?~ diff --git a/docs/images/readsimulator_workflow.svg b/docs/images/readsimulator_workflow.svg index 8bd6dea..95488ec 100755 --- a/docs/images/readsimulator_workflow.svg +++ b/docs/images/readsimulator_workflow.svg @@ -1,4 +1,4 @@ -
    Fasta
    Fasta
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    Wgsim
    Wgsim
    InSilicoSeq
    InSilicoSeq
    Whole genome
    Whole genome
    Metagenome
    Metagenome
    Amplicon
    Amplicon
    Target capture
    Target capture
    CRABS
    CRABS
    ART
    ART
    Bowtie2
    Bowtie2
    Samtools
    Samtools
    CapSim
    CapSim
    Create Samplesheet
    Create Sam...
    Fastqc
    Fastqc
    Multiqc
    Multiqc
    Legend
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    Reference
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    Probes
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    Simulated Reads
    Simulated Reads
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    nf-core/readsimulator
    nf-core/readsimulator
    1.0.0
    1.0.0    Text is not SVG - cannot display     \ No newline at end of file +
    Fasta
    Fasta
    Fasta
    Fasta
    Wgsim
    Wgsim
    InSilicoSeq
    InSilicoSeq
    Whole genome
    Whole genome
    Metagenome
    Metagenome
    Amplicon
    Amplicon
    Target capture
    Target capture
    CRABS
    CRABS
    ART
    ART
    Bowtie2
    Bowtie2
    Samtools
    Samtools
    CapSim
    CapSim
    Create Samplesheet
    Create Sam...
    Fastqc
    Fastqc
    Multiqc
    Multiqc
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    Reference
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    nf-core/
    readsimulator
    nf-core/...
    1.0.0
    1.0.0    Text is not SVG - cannot display     \ No newline at end of file diff --git a/docs/output.md b/docs/output.md index a9f0e49..84116f3 100644 --- a/docs/output.md +++ b/docs/output.md @@ -10,17 +10,17 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -- [ART](#art) - ART Illumina simulated reads -- [Bowtie2](#bowtie2) - Bowtie2 alignments and SAMtools index -- [CapSim](#capsim) - CapSim simulated reads (Illumina or Pacbio) -- [CRABS](#crabs) - Formated reference database for simulating amplicon reads +- [ART](#art) - Simulated amplicon reads +- [Bowtie2](#bowtie2) - Alignments and index files +- [CapSim](#capsim) - Simulated target capture reads +- [CRABS](#crabs) - Reference database formatted for amplicon read simulation - [FastQC](#fastqc) - Raw read QC -- [InSilicoSeq](#insilicoseq) - InSilicoSeq simulated metagenomic reads +- [InSilicoSeq](#insilicoseq) - Simulated metagenomic reads - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution - [Samplesheet](#samplesheet) - Samplesheets produced during the running of the pipeline - [Unzip](#unzip) - Unziped probe file -- [Wgsim](#wgsim) - Wgsim simulated wholegenome reads +- [Wgsim](#wgsim) - Simulated wholegenome reads ### ART @@ -74,7 +74,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d

    Output files - `crabs_dbdownload/` - - `*.fasta`: Reference fasta file. + - `*.fa`: Reference fasta file. - `crabs_dbimport/` - `*.fa`: Reference fasta file. - `crabs_insilicopcr/` diff --git a/modules/local/crabs/dbdownload/main.nf b/modules/local/crabs/dbdownload/main.nf index 6db93f0..55414b0 100644 --- a/modules/local/crabs/dbdownload/main.nf +++ b/modules/local/crabs/dbdownload/main.nf @@ -22,13 +22,12 @@ process CRABS_DBDOWNLOAD { task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' - def ncbi_input = amplicon_ncbi_db ? "--source ncbi --database ${amplicon_ncbi_db} --output ncbi.fasta" : "" - def embl_input = amplicon_embl_db ? "--source embl --database ${params.amplicon_embl_db} --output embl.fasta " : "" - def bold_input = amplicon_bold_db ? "--source bold --database '${params.amplicon_bold_db} --output bold.fasta" : "" - // Check for clashing inputs here - - def input_args = ncbi_input + embl_input + bold_input + def args = task.ext.args ?: '' + def input_args = amplicon_ncbi_db ? "--source ncbi --database ${params.amplicon_ncbi_db} --output ncbi.fa" : + amplicon_embl_db ? "--source embl --database ${params.amplicon_embl_db} --output embl.fa " : + amplicon_bold_db ? "--source bold --database ${params.amplicon_bold_db} --output bold.fa" : + "" + def VERSION = '0.1.1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. """ crabs db_download \\ diff --git a/modules/local/wgsim/main.nf b/modules/local/wgsim/main.nf index e664911..6671598 100644 --- a/modules/local/wgsim/main.nf +++ b/modules/local/wgsim/main.nf @@ -24,17 +24,14 @@ process WGSIM { def seed = task.ext.seed ?: "${meta.seed}" def VERSION = '1.0' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. """ - seed=\$(echo $seed | sed 's/\\[//g' | sed 's/\\]//g') - prefix=\$(echo $prefix | sed 's/\\[//g' | sed 's/\\]//g') - wgsim \\ $args \\ - -S \$seed \\ + -S $seed \\ $fasta \\ - \${prefix}_R1.fq \\ - \${prefix}_R2.fq + ${prefix}_R1.fq \\ + ${prefix}_R2.fq - gzip \${prefix}_R1.fq \${prefix}_R2.fq + gzip ${prefix}_R1.fq ${prefix}_R2.fq cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/nextflow.config b/nextflow.config index 231ce3a..411f044 100644 --- a/nextflow.config +++ b/nextflow.config @@ -18,10 +18,10 @@ params { fasta = null // Simulation options - amplicon = null - target_capture = null - metagenome = null - wholegenome = null + amplicon = false + target_capture = false + metagenome = false + wholegenome = false // Amplicon options fw_primer = 'GTCGGTAAAACTCGTGCCAGC' diff --git a/nextflow_schema.json b/nextflow_schema.json index de6ce4f..d57b9c3 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -52,21 +52,25 @@ "amplicon": { "type": "boolean", "description": "Option to simulate amplicon sequencing reads.", + "default": false, "fa_icon": "fas fa-question-circle" }, "target_capture": { "type": "boolean", "description": "Option to simulate target capture sequencing reads.", + "default": false, "fa_icon": "fas fa-question-circle" }, "metagenome": { "type": "boolean", "description": "Option to simulate metagenomic sequencing reads.", + "default": false, "fa_icon": "fas fa-question-circle" }, "wholegenome": { "type": "boolean", "description": "Option to simulate wholegenomic sequencing reads.", + "default": false, "fa_icon": "fas fa-question-circle" } } @@ -79,12 +83,12 @@ "properties": { "fw_primer": { "type": "string", - "description": "Forward primer to use with crabs_insilicopcr. E.g., GTCGGTAAAACTCGTGCCAGC", + "description": "Forward primer to use with crabs_insilicopcr.", "default": "GTCGGTAAAACTCGTGCCAGC" }, "rv_primer": { "type": "string", - "description": "Reverse primer to use with crabs_insilicopcr. E.g., CATAGTGGGGTATCTAATCCCAGTTTG", + "description": "Reverse primer to use with crabs_insilicopcr.", "default": "CATAGTGGGGTATCTAATCCCAGTTTG" }, "amplicon_ref_source": { @@ -182,7 +186,7 @@ }, "target_capture_fmedian": { "type": "integer", - "default": 2000, + "default": 500, "description": "Median of fragment size at shearing." }, "target_capture_fshape": { @@ -210,12 +214,12 @@ }, "target_capture_num": { "type": "integer", - "default": 1000000, + "default": 500000, "description": "Number of fragments." }, "target_capture_illen": { "type": "integer", - "default": 300, + "default": 150, "description": "Illumina: read length." }, "target_capture_pblen": { diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index f6612c1..30c812e 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -21,7 +21,11 @@ workflow AMPLICON_WORKFLOW { // MODULE: Run Crabs db_download if user doesn't have a reference database // if ( !params.fasta ) { - CRABS_DBDOWNLOAD() + CRABS_DBDOWNLOAD ( + params.amplicon_ncbi_db, + params.amplicon_embl_db, + params.amplicon_bold_db + ) ch_versions = ch_versions.mix(CRABS_DBDOWNLOAD.out.versions) ch_ref_fasta = CRABS_DBDOWNLOAD.out.fasta .map { diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index ef048b6..b9d9547 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -39,6 +39,7 @@ include { INSILICOSEQ_GENERATE } from '../modules/local/insilicoseq/generate/ include { CREATE_SAMPLESHEET } from '../modules/local/custom/create_samplesheet/main' include { MERGE_SAMPLESHEETS } from '../modules/local/custom/merge_samplesheets/main' include { WGSIM } from '../modules/local/wgsim/main' +include { NCBIGENOMEDOWNLOAD } from '../modules/local/ncbi_genome_download' // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules @@ -77,7 +78,8 @@ workflow READSIMULATOR { if ( params.fasta ) { ch_fasta = Channel.fromPath(params.fasta) } else { - ch_fasta = Channel.empty() + //ch_fasta = Channel.empty() + ch_fasta = NCBIGENOMEDOWNLOAD(ch_input.first(), [], []).fna } if ( params.probe_fasta ) { From d6777d998c1187f6143905931a9eabbd1b2a90ee Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 11 Jan 2024 02:41:12 +0000 Subject: [PATCH 60/89] Added ncbi-genome-download and bedtools_getfasta --- CHANGELOG.md | 1 + conf/modules.config | 24 +++++ docs/output.md | 22 +++++ lib/WorkflowReadsimulator.groovy | 4 +- modules.json | 10 ++ .../local/custom/merge_fastas/environment.yml | 7 ++ modules/local/custom/merge_fastas/main.nf | 31 ++++++ .../local/uncompress_fasta/environment.yml | 7 ++ modules/local/uncompress_fasta/main.nf | 33 +++++++ .../nf-core/bedtools/getfasta/environment.yml | 7 ++ modules/nf-core/bedtools/getfasta/main.nf | 37 +++++++ modules/nf-core/bedtools/getfasta/meta.yml | 36 +++++++ .../ncbigenomedownload/environment.yml | 7 ++ modules/nf-core/ncbigenomedownload/main.nf | 55 +++++++++++ modules/nf-core/ncbigenomedownload/meta.yml | 96 +++++++++++++++++++ nextflow.config | 18 ++-- nextflow_schema.json | 87 +++++++---------- subworkflows/local/amplicon_workflow.nf | 41 +++----- subworkflows/local/target_capture_workflow.nf | 54 +++++++---- workflows/readsimulator.nf | 40 ++++++-- 20 files changed, 497 insertions(+), 120 deletions(-) create mode 100644 modules/local/custom/merge_fastas/environment.yml create mode 100644 modules/local/custom/merge_fastas/main.nf create mode 100644 modules/local/uncompress_fasta/environment.yml create mode 100644 modules/local/uncompress_fasta/main.nf create mode 100644 modules/nf-core/bedtools/getfasta/environment.yml create mode 100644 modules/nf-core/bedtools/getfasta/main.nf create mode 100644 modules/nf-core/bedtools/getfasta/meta.yml create mode 100644 modules/nf-core/ncbigenomedownload/environment.yml create mode 100644 modules/nf-core/ncbigenomedownload/main.nf create mode 100644 modules/nf-core/ncbigenomedownload/meta.yml diff --git a/CHANGELOG.md b/CHANGELOG.md index 5fb714a..5b09bbd 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -14,6 +14,7 @@ Initial release of nf-core/readsimulator, created with the [nf-core](https://nf- - Option to simulate metagenomic sequencing reads - Option to simulate wholegenome sequencing reads - Samplesheet creation after reads have been simulated +- ncbi-genome-download added to download reference database ### `Fixed` diff --git a/conf/modules.config b/conf/modules.config index 70f8f8f..920f118 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -128,6 +128,14 @@ process { ] } + withName: MERGE_FASTAS { + publishDir = [ + path: { "${params.outdir}/merged_fastas" }, + mode: params.publish_dir_mode, + enabled: false + ] + } + withName: MERGE_SAMPLESHEETS { publishDir = [ path: { "${params.outdir}/samplesheet" }, @@ -144,6 +152,14 @@ process { ] } + withName: NCBIGENOMEDOWNLOAD { + ext.args = " -N --formats fasta" + publishDir = [ + path: { "${params.outdir}/ncbigenomedownload" }, + mode: params.publish_dir_mode + ] + } + withName: SAMTOOLS_INDEX { publishDir = [ path: { "${params.outdir}/bowtie2" }, @@ -151,6 +167,14 @@ process { ] } + withName: UNCOMPRESS_FASTA { + publishDir = [ + path: { "${params.outdir}/uncompress_fasta" }, + mode: params.publish_dir_mode, + enabled: false + ] + } + withName: UNZIP_PROBE { publishDir = [ path: { "${params.outdir}/probes" }, diff --git a/docs/output.md b/docs/output.md index c0936d8..29c2b54 100644 --- a/docs/output.md +++ b/docs/output.md @@ -11,12 +11,14 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: - [ART](#art) - Simulated amplicon reads +- [bedtools](#bedtools) - Probe fasta file - [Bowtie2](#bowtie2) - Alignments and index files - [CapSim](#capsim) - Simulated target capture reads - [CRABS](#crabs) - Reference database formatted for amplicon read simulation - [FastQC](#fastqc) - Raw read QC - [InSilicoSeq](#insilicoseq) - Simulated metagenomic reads - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline +- [ncbi-genome-download](#ncbigenomedownload) - Reference fasta files - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution - [Samplesheet](#samplesheet) - Samplesheets produced during the running of the pipeline - [Unzip](#unzip) - Unziped probe file @@ -33,6 +35,16 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d +### bedtools + +
    +Output files + +- `bedtools/` + - `*.fa`: The probe fasta file extracted from the reference fasta file if the input probe file was a bed file. + +
    + [ART](https://www.niehs.nih.gov/research/resources/software/biostatistics/art/index.cfm) is a tool for simulating Illumina sequencing reads. For further reading and documentation see the [ART Illumina manual](https://manpages.debian.org/testing/art-nextgen-simulation-tools/art_illumina.1.en.html). ### Bowtie2 @@ -107,6 +119,16 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. ::: +### ncbi-genome-download + +
    +Output files + +- `ncbigenomedownload/` + - `*.fna.gz`: Reference fasta files downloaded from NCBI + +
    + ### InSilicoSeq
    diff --git a/lib/WorkflowReadsimulator.groovy b/lib/WorkflowReadsimulator.groovy index cfe8b1b..c0b9df0 100755 --- a/lib/WorkflowReadsimulator.groovy +++ b/lib/WorkflowReadsimulator.groovy @@ -14,8 +14,8 @@ class WorkflowReadsimulator { genomeExistsError(params, log) - if (!params.fasta && params.target_capture) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." + if (!params.fasta && !params.ncbidownload_accessions && !params.ncbidownload_taxids) { + Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file. Alternatively, '--ncbidownload_accessions accessions.txt' or '--ncbidownload_taxids taxids.txt' can be used." } } diff --git a/modules.json b/modules.json index c49c80b..1684926 100644 --- a/modules.json +++ b/modules.json @@ -11,6 +11,11 @@ "installed_by": ["modules"], "patch": "modules/nf-core/art/illumina/art-illumina.diff" }, + "bedtools/getfasta": { + "branch": "master", + "git_sha": "3b248b84694d1939ac4bb33df84bf6233a34d668", + "installed_by": ["modules"] + }, "bowtie2/align": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", @@ -36,6 +41,11 @@ "git_sha": "4ab13872435962dadc239979554d13709e20bf29", "installed_by": ["modules"] }, + "ncbigenomedownload": { + "branch": "master", + "git_sha": "4be1afba55bca38b29b44767b699ca7996f26d4d", + "installed_by": ["modules"] + }, "samtools/index": { "branch": "master", "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8", diff --git a/modules/local/custom/merge_fastas/environment.yml b/modules/local/custom/merge_fastas/environment.yml new file mode 100644 index 0000000..801016a --- /dev/null +++ b/modules/local/custom/merge_fastas/environment.yml @@ -0,0 +1,7 @@ +name: merge_fastas +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - conda-forge::sed=4.7 diff --git a/modules/local/custom/merge_fastas/main.nf b/modules/local/custom/merge_fastas/main.nf new file mode 100644 index 0000000..dd77416 --- /dev/null +++ b/modules/local/custom/merge_fastas/main.nf @@ -0,0 +1,31 @@ +process MERGE_FASTAS { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'nf-core/ubuntu:20.04' }" + + input: + tuple val(meta), path(fasta) + + output: + tuple val(meta), path("*.fa.gz"), emit: fasta + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + cat ${args} *.gz > ${prefix}.fa.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + cat: \$(cat --version | head -n 1 | sed 's/cat (GNU coreutils) //g') + END_VERSIONS + """ +} diff --git a/modules/local/uncompress_fasta/environment.yml b/modules/local/uncompress_fasta/environment.yml new file mode 100644 index 0000000..334d0a3 --- /dev/null +++ b/modules/local/uncompress_fasta/environment.yml @@ -0,0 +1,7 @@ +name: gzip +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - conda-forge::gzip=1.11 diff --git a/modules/local/uncompress_fasta/main.nf b/modules/local/uncompress_fasta/main.nf new file mode 100644 index 0000000..56edf1a --- /dev/null +++ b/modules/local/uncompress_fasta/main.nf @@ -0,0 +1,33 @@ +process UNCOMPRESS_FASTA { + tag "$file" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gzip:1.11': + 'biocontainers/gzip:1.11' }" + + input: + path(fasta) + + output: + path "${fasta.name.replace('.gz', '')}", emit: fasta + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def is_compressed = fasta.name.endsWith(".gz") + def fasta_name = fasta.name.replace(".gz", "") + """ + if [ "${is_compressed}" == "true" ]; then + gzip -c -d ${fasta} > ${fasta_name} + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gzip: \$(gzip -V | head -n 1 | sed 's/gzip //g') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bedtools/getfasta/environment.yml b/modules/nf-core/bedtools/getfasta/environment.yml new file mode 100644 index 0000000..a89401f --- /dev/null +++ b/modules/nf-core/bedtools/getfasta/environment.yml @@ -0,0 +1,7 @@ +name: bedtools_getfasta +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bedtools=2.31.1 diff --git a/modules/nf-core/bedtools/getfasta/main.nf b/modules/nf-core/bedtools/getfasta/main.nf new file mode 100644 index 0000000..50549c7 --- /dev/null +++ b/modules/nf-core/bedtools/getfasta/main.nf @@ -0,0 +1,37 @@ +process BEDTOOLS_GETFASTA { + tag "$bed" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bedtools:2.31.1--hf5e1c6e_0' : + 'biocontainers/bedtools:2.31.1--hf5e1c6e_0' }" + + input: + path bed + path fasta + + output: + path "*.fa" , emit: fasta + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${bed.baseName}" + """ + bedtools \\ + getfasta \\ + $args \\ + -fi $fasta \\ + -bed $bed \\ + -fo ${prefix}.fa + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/bedtools/getfasta/meta.yml b/modules/nf-core/bedtools/getfasta/meta.yml new file mode 100644 index 0000000..25fb19a --- /dev/null +++ b/modules/nf-core/bedtools/getfasta/meta.yml @@ -0,0 +1,36 @@ +name: bedtools_getfasta +description: extract sequences in a FASTA file based on intervals defined in a feature file. +keywords: + - bed + - fasta + - getfasta +tools: + - bedtools: + description: | + A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. + documentation: https://bedtools.readthedocs.io/en/latest/content/tools/intersect.html + licence: ["MIT"] +input: + - bed: + type: file + description: Bed feature file + pattern: "*.{bed}" + - fasta: + type: file + description: Input fasta file + pattern: "*.{fa,fasta}" +output: + - fasta: + type: file + description: Output fasta file with extracted sequences + pattern: "*.{fa}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" +maintainers: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/ncbigenomedownload/environment.yml b/modules/nf-core/ncbigenomedownload/environment.yml new file mode 100644 index 0000000..746c608 --- /dev/null +++ b/modules/nf-core/ncbigenomedownload/environment.yml @@ -0,0 +1,7 @@ +name: ncbigenomedownload +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::ncbi-genome-download=0.3.3 diff --git a/modules/nf-core/ncbigenomedownload/main.nf b/modules/nf-core/ncbigenomedownload/main.nf new file mode 100644 index 0000000..baa0b51 --- /dev/null +++ b/modules/nf-core/ncbigenomedownload/main.nf @@ -0,0 +1,55 @@ +process NCBIGENOMEDOWNLOAD { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ncbi-genome-download:0.3.3--pyh7cba7a3_0' : + 'biocontainers/ncbi-genome-download:0.3.3--pyh7cba7a3_0' }" + + input: + val meta + path accessions + path taxids + val groups + + output: + tuple val(meta), path("*_genomic.gbff.gz") , emit: gbk , optional: true + tuple val(meta), path("*_genomic.fna.gz") , emit: fna , optional: true + tuple val(meta), path("*_rm.out.gz") , emit: rm , optional: true + tuple val(meta), path("*_feature_table.txt.gz") , emit: features, optional: true + tuple val(meta), path("*_genomic.gff.gz") , emit: gff , optional: true + tuple val(meta), path("*_protein.faa.gz") , emit: faa , optional: true + tuple val(meta), path("*_protein.gpff.gz") , emit: gpff , optional: true + tuple val(meta), path("*_wgsmaster.gbff.gz") , emit: wgs_gbk , optional: true + tuple val(meta), path("*_cds_from_genomic.fna.gz"), emit: cds , optional: true + tuple val(meta), path("*_rna.fna.gz") , emit: rna , optional: true + tuple val(meta), path("*_rna_from_genomic.fna.gz"), emit: rna_fna , optional: true + tuple val(meta), path("*_assembly_report.txt") , emit: report , optional: true + tuple val(meta), path("*_assembly_stats.txt") , emit: stats , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def accessions_opt = accessions ? "-A ${accessions}" : "" + def taxids_opt = taxids ? "-t ${taxids}" : "" + """ + ncbi-genome-download \\ + $args \\ + $accessions_opt \\ + $taxids_opt \\ + --output-folder ./ \\ + --flat-output \\ + --parallel $task.cpus \\ + $groups + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ncbigenomedownload: \$( ncbi-genome-download --version ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/ncbigenomedownload/meta.yml b/modules/nf-core/ncbigenomedownload/meta.yml new file mode 100644 index 0000000..e7d3720 --- /dev/null +++ b/modules/nf-core/ncbigenomedownload/meta.yml @@ -0,0 +1,96 @@ +name: ncbigenomedownload +description: A tool to quickly download assemblies from NCBI's Assembly database +keywords: + - fasta + - download + - assembly +tools: + - ncbigenomedownload: + description: Download genome files from the NCBI FTP server. + homepage: https://github.com/kblin/ncbi-genome-download + documentation: https://github.com/kblin/ncbi-genome-download + tool_dev_url: https://github.com/kblin/ncbi-genome-download + licence: ["Apache Software License"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - accessions: + type: file + description: List of accessions (one per line) to download + pattern: "*.txt" + - taxids: + type: file + description: List of taxids (one per line) to download + pattern: "*.txt" + - groups: + type: string + description: NCBI taxonomic groups to download. Can be a comma-separated list. Options are ['all', 'archaea', 'bacteria', 'fungi', 'invertebrate', 'metagenomes', 'plant', 'protozoa', 'vertebrate_mammalian', 'vertebrate_other', 'viral'] +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - gbk: + type: file + description: GenBank format of the genomic sequence(s) in the assembly + pattern: "*_genomic.gbff.gz" + - fna: + type: file + description: FASTA format of the genomic sequence(s) in the assembly. + pattern: "*_genomic.fna.gz" + - rm: + type: file + description: RepeatMasker output for eukaryotes. + pattern: "*_rm.out.gz" + - features: + type: file + description: Tab-delimited text file reporting locations and attributes for a subset of annotated features + pattern: "*_feature_table.txt.gz" + - gff: + type: file + description: Annotation of the genomic sequence(s) in GFF3 format + pattern: "*_genomic.gff.gz" + - faa: + type: file + description: FASTA format of the accessioned protein products annotated on the genome assembly. + pattern: "*_protein.faa.gz" + - gpff: + type: file + description: GenPept format of the accessioned protein products annotated on the genome assembly. + pattern: "*_protein.gpff.gz" + - wgs_gbk: + type: file + description: GenBank flat file format of the WGS master for the assembly + pattern: "*_wgsmaster.gbff.gz" + - cds: + type: file + description: FASTA format of the nucleotide sequences corresponding to all CDS features annotated on the assembly + pattern: "*_cds_from_genomic.fna.gz" + - rna: + type: file + description: FASTA format of accessioned RNA products annotated on the genome assembly + pattern: "*_rna.fna.gz" + - rna_fna: + type: file + description: FASTA format of the nucleotide sequences corresponding to all RNA features annotated on the assembly + pattern: "*_rna_from_genomic.fna.gz" + - report: + type: file + description: Tab-delimited text file reporting the name, role and sequence accession.version for objects in the assembly + pattern: "*_assembly_report.txt" + - stats: + type: file + description: Tab-delimited text file reporting statistics for the assembly + pattern: "*_assembly_stats.txt" +authors: + - "@rpetit3" +maintainers: + - "@rpetit3" diff --git a/nextflow.config b/nextflow.config index e2cb2bf..3677248 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,6 +16,10 @@ params { igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false fasta = null + ncbidownload_accessions = null + ncbidownload_taxids = null + ncbidownload_group = "all" + ncbidownload_section = "refseq" // Simulation options amplicon = false @@ -24,20 +28,14 @@ params { wholegenome = false // Amplicon options - fw_primer = 'GTCGGTAAAACTCGTGCCAGC' - rv_primer = 'CATAGTGGGGTATCTAATCCCAGTTTG' - amplicon_ref_source = 'bold' - amplicon_ncbi_db = 'nucleotide' - amplicon_ncbi_query = '16S[All Fields] AND ("1"[SLEN] : "50000"[SLEN])' - amplicon_ncbi_batchsize = 5000 - amplicon_embl_db = 'vrt*' - amplicon_bold_db = 'Actinopterygii|Aves' + amplicon_fw_primer = 'GTCGGTAAAACTCGTGCCAGC' + amplicon_rv_primer = 'CATAGTGGGGTATCTAATCCCAGTTTG' amplicon_read_count = 500 amplicon_read_length = 130 amplicon_seq_system = 'HS25' // Target capture options - probe_fasta = null + probe_file = null probe_ref_name = 'Tetrapods-UCE-5Kv1' target_capture_mode = 'illumina' target_capture_fmedian = 500 @@ -52,8 +50,6 @@ params { target_capture_ilmode = 'pe' // Metagenome options - metagenome_ncbi_db = 'viruses' - metagenome_n_genomes = '10' metagenome_abundance = 'lognormal' metagenome_abundance_file = null metagenome_coverage = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 86653af..08ddcd2 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -81,49 +81,16 @@ "fa_icon": "fas fa-dna", "description": "Options for simulating amplicon sequencing reads.", "properties": { - "fw_primer": { + "amplicon_fw_primer": { "type": "string", "description": "Forward primer to use with crabs_insilicopcr.", "default": "GTCGGTAAAACTCGTGCCAGC" }, - "rv_primer": { + "amplicon_rv_primer": { "type": "string", "description": "Reverse primer to use with crabs_insilicopcr.", "default": "CATAGTGGGGTATCTAATCCCAGTTTG" }, - "amplicon_ref_source": { - "type": "string", - "description": "Source for downloading reference database when using `--amplicon` parameter, but not using `--fasta` parameter.", - "help_text": "Can be 'ncbi', 'embl', or 'bold'.", - "default": "bold", - "enum": ["ncbi", "embl", "bold"] - }, - "amplicon_ncbi_db": { - "type": "string", - "description": "NCBI database to use when `--amplicon_ref_source` is set to 'ncbi'", - "default": "nucleotide" - }, - "amplicon_ncbi_query": { - "type": "string", - "description": "NCBI query to use when `--amplicon_ref_source` is set to 'ncbi'", - "default": "16S[All Fields] AND (\"1\"[SLEN] : \"50000\"[SLEN])", - "help_text": "Multiple species can be queried like this '(\"Mus musculus\"[Organism] OR \"Salmo salar\"[Organism]) AND 16S[All Fields]'" - }, - "amplicon_ncbi_batchsize": { - "type": "integer", - "description": "Batchsize to use when `--amplicon_ref_source` is set to 'ncbi'", - "default": 5000 - }, - "amplicon_embl_db": { - "type": "string", - "description": "EMBL database to use when `--amplicon_ref_source` is set to 'embl'", - "default": "vrt*" - }, - "amplicon_bold_db": { - "type": "string", - "description": "BOLD database to use when `--amplicon_ref_source` is set to 'bold'. Separate database names with `|`", - "default": "Actinopterygii|Aves" - }, "amplicon_read_count": { "type": "integer", "description": "Number of reads to be simulated per amplicon.", @@ -138,8 +105,8 @@ "type": "string", "description": "Sequencing system of reads to be simulated.", "default": "HS25", - "help_text": "Can be 'GA1' for Genome Analyser I, 'GA2' for Genome Analyser II, 'HS10' for HiSeq 1000, 'HS20' for HiSeq 2000, 'HS25' for HiSeq 2500, or 'MS' for MiSeq.", - "enum": ["GA1", "GA2", "HS10", "HS20", "HS25", "MS"] + "help_text": "Can be 'GA1' for Genome Analyser I, 'GA2' for Genome Analyser II, 'HS10' for HiSeq 1000, 'HS20' for HiSeq 2000, 'HS25' for HiSeq 2500, 'HSXn' for HiSeqX PCR free, 'HSXt' for HiSeqX TruSeq, 'MinS' for MiniSeq TruSeq, 'MSv1' for MiSeq v1, 'MSv3' for MiSeq v3, or 'NS50' for NextSeq500 v2.", + "enum": ["GA1", "GA2", "HS10", "HS20", "HS25", "HSXn", "HSXt", "MinS", "MSv1", "MSv3", "NS50"] } } }, @@ -149,13 +116,12 @@ "fa_icon": "fas fa-dna", "description": "Options for simulating target capture sequencing reads.", "properties": { - "probe_fasta": { + "probe_file": { "type": "string", "format": "file-path", "exists": true, "mimetype": "text/plain", - "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", - "description": "Path to FASTA probe file.", + "description": "Path to bait/probe file. Can be a fasta file or a bed file.", "help_text": "This parameter is *mandatory* if `--probe_ref_name` is not specified but `--target_capture` is specified.", "fa_icon": "far fa-file-code" }, @@ -242,18 +208,6 @@ "fa_icon": "fas fa-dna", "description": "Options for simulating metagenomic sequencing reads.", "properties": { - "metagenome_ncbi_db": { - "type": "string", - "default": "viruses", - "description": "NCBI databases supported by InSilicoSeq. This option gets used when not using the `--fasta` or `--genome` parameters.", - "help_text": "Supported databases are 'bacteria', 'viruses', and 'archaea'. Multiple databases can be used by providing a space seperated string." - }, - "metagenome_n_genomes": { - "type": "string", - "default": "10", - "description": "Number of genomes to download form NCBI. This option gets used when not using the `--fasta` or `--genome` parameters.", - "help_text": "Use multiple values (space seperated) if using multiple NCBI databases" - }, "metagenome_abundance": { "type": "string", "default": "lognormal", @@ -382,7 +336,7 @@ "mimetype": "text/plain", "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", "description": "Path to reference FASTA file.", - "help_text": "If this parameter is not used, the pipeline will download a fasta file, either using the `--genome` parameter or by using other parameters specific to individual modes (e.g., the `--amplicon` mode has parameters specific to just that mode.).", + "help_text": "If this parameter is not used, the pipeline will download a fasta file, either using the `--genome` parameter or by using ncbi-genome-download (relevant parameters for ncbi-genome-download all start with `--ncbidownload_`).", "fa_icon": "far fa-file-code" }, "igenomes_ignore": { @@ -391,6 +345,33 @@ "fa_icon": "fas fa-ban", "hidden": true, "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + }, + "ncbidownload_accessions": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/plain", + "description": "Path to text file containing accession ids (one accession per row)." + }, + "ncbidownload_taxids": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/plain", + "description": "Path to text file containing taxids (one taxid per row).", + "help_text": "" + }, + "ncbidownload_group": { + "type": "string", + "default": "all", + "description": "The NCBI taxonomic groups to download. Options include 'all', 'archaea', 'bacteria', 'fungi', 'invertebrate', 'metagenomes', 'plant', 'protozoa', 'vertebrate_mammalian', 'vertebrate_other', and 'viral'. A comma-separated list is also valid (e.g., 'bacteria,viral').", + "enum": ["all", "archaea", "bacteria", "fungi", "invertebrate", "metagenomes", "plant", "protozoa", "vertebrate_mammalian", "vertebrate_other", "viral"] + }, + "ncbidownload_section": { + "type": "string", + "default": "refseq", + "description": "The NCBI section to download. 'refseq' or 'genbank'.", + "enum": ["refseq", "genbank"] } } }, diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index 30c812e..1073768 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -2,7 +2,6 @@ // Simulate amplicon reads // -include { CRABS_DBDOWNLOAD } from '../../modules/local/crabs/dbdownload/main' include { CRABS_DBIMPORT } from '../../modules/local/crabs/dbimport/main' include { CRABS_INSILICOPCR } from '../../modules/local/crabs/insilicopcr/main' include { ART_ILLUMINA } from '../../modules/nf-core/art/illumina/main' @@ -18,37 +17,19 @@ workflow AMPLICON_WORKFLOW { ch_versions = Channel.empty() // - // MODULE: Run Crabs db_download if user doesn't have a reference database + // MODULE: Run Crabs db_import // - if ( !params.fasta ) { - CRABS_DBDOWNLOAD ( - params.amplicon_ncbi_db, - params.amplicon_embl_db, - params.amplicon_bold_db - ) - ch_versions = ch_versions.mix(CRABS_DBDOWNLOAD.out.versions) - ch_ref_fasta = CRABS_DBDOWNLOAD.out.fasta - .map { - fasta -> - return [ [id:"amplicon"], fasta ] - } - - // - // MODULE: Run Crabs db_import if user does have a reference database - // - } else { - ch_meta_fasta = Channel.fromPath(params.fasta) - .map { - fasta -> - return [ [id:"amplicon"], fasta ] - } + ch_meta_fasta = ch_fasta + .map { + fasta -> + return [ [id:"amplicon"], fasta ] + } - CRABS_DBIMPORT ( - ch_meta_fasta - ) - ch_versions = ch_versions.mix(CRABS_DBIMPORT.out.versions) - ch_ref_fasta = CRABS_DBIMPORT.out.fasta - } + CRABS_DBIMPORT ( + ch_meta_fasta + ) + ch_versions = ch_versions.mix(CRABS_DBIMPORT.out.versions) + ch_ref_fasta = CRABS_DBIMPORT.out.fasta // // MODULE: Run Crabs insilico_pcr diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index 8dbd736..53c0f63 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -2,11 +2,13 @@ // Simulate UCE target capture reads // +include { BEDTOOLS_GETFASTA } from '../../modules/nf-core/bedtools/getfasta/main' include { BOWTIE2_BUILD } from '../../modules/nf-core/bowtie2/build/main' include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/main' include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' include { JAPSA_CAPSIM } from '../../modules/local/japsa/capsim/main' -include { UNZIP as UNZIP_PROBE } from '../../modules/local/unzip/main' +include { UNZIP } from '../../modules/local/unzip/main' +include { UNCOMPRESS_FASTA } from '../../modules/local/uncompress_fasta/main' workflow TARGET_CAPTURE_WORKFLOW { take: @@ -17,29 +19,33 @@ workflow TARGET_CAPTURE_WORKFLOW { main: ch_versions = Channel.empty() - ch_meta_fasta = ch_fasta - .map { - fasta -> - return [ [id:"target_capture"], fasta ] - } - - // - // MODULE: Create Bowtie index - // - BOWTIE2_BUILD ( - ch_meta_fasta - ) - ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions.first()) - // // MODULE: Unzip probes file if user is downloading a reference probe file // - if ( !params.probe_fasta ) { + if ( !params.probe_file ) { ch_zip_file = Channel.fromPath(params.probe_ref_db[params.probe_ref_name]["url"]) - ch_probes = UNZIP_PROBE ( + ch_probes = UNZIP ( ch_zip_file ).file } + + // + // MODULE: Run bedtools_getfasta if the probe file is a bed file + // + if ( params.probe_file.endsWith('.bed') ) { + // Bedtools_getfasta requires an uncompressed fasta file + ch_uncompressed_fasta = UNCOMPRESS_FASTA ( + ch_fasta + ).fasta + + BEDTOOLS_GETFASTA ( + ch_probes, + ch_uncompressed_fasta + ) + + ch_probes = BEDTOOLS_GETFASTA.out.fasta + } + ch_probes = ch_probes .map { fasta -> @@ -49,6 +55,20 @@ workflow TARGET_CAPTURE_WORKFLOW { [ meta, fasta ] } + ch_meta_fasta = ch_fasta + .map { + fasta -> + return [ [id:"target_capture"], fasta ] + } + + // + // MODULE: Create Bowtie index + // + BOWTIE2_BUILD ( + ch_meta_fasta + ) + ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions.first()) + // // MODULE: Align probes to genome // diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 75c3ece..79c1c7c 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -35,11 +35,11 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // MODULE: Local modules // +include { MERGE_FASTAS } from '../modules/local/custom/merge_fastas/main' include { INSILICOSEQ_GENERATE } from '../modules/local/insilicoseq/generate/main' include { CREATE_SAMPLESHEET } from '../modules/local/custom/create_samplesheet/main' include { MERGE_SAMPLESHEETS } from '../modules/local/custom/merge_samplesheets/main' include { WGSIM } from '../modules/local/wgsim/main' -include { NCBIGENOMEDOWNLOAD } from '../modules/local/ncbi_genome_download' // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules @@ -57,6 +57,7 @@ include { TARGET_CAPTURE_WORKFLOW } from '../subworkflows/local/target_capture_w // MODULE: Installed directly from nf-core/modules // include { FASTQC } from '../modules/nf-core/fastqc/main' +include { NCBIGENOMEDOWNLOAD } from '../modules/nf-core/ncbigenomedownload/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' @@ -74,16 +75,41 @@ workflow READSIMULATOR { ch_versions = Channel.empty() ch_input = Channel.fromSamplesheet("input") ch_simulated_reads = Channel.empty() + ch_taxids = Channel.empty() + ch_accessions = Channel.empty() if ( params.fasta ) { ch_fasta = Channel.fromPath(params.fasta) } else { - //ch_fasta = Channel.empty() - ch_fasta = NCBIGENOMEDOWNLOAD(ch_input.first(), [], []).fna + if ( params.ncbidownload_accessions ) { + ch_accessions = Channel.fromPath(params.ncbidownload_accessions) + } else if ( params.ncbidownload_taxids ) { + ch_taxids = Channel.fromPath(params.ncbidownload_taxids) + } + + // + // MODULE: Download reference fasta files + // + NCBIGENOMEDOWNLOAD ( + [ id:"ncbigenomedownload" ], + ch_accessions.ifEmpty([]), + ch_taxids.ifEmpty([]), + params.ncbidownload_group + ) + + MERGE_FASTAS ( + NCBIGENOMEDOWNLOAD.out.fna + ) + + ch_fasta = MERGE_FASTAS.out.fasta + .map { + meta, fasta -> + return fasta + } } - if ( params.probe_fasta ) { - ch_probes = Channel.fromPath(params.probe_fasta) + if ( params.probe_file ) { + ch_probes = Channel.fromPath(params.probe_file) } else { ch_probes = Channel.empty() } @@ -93,7 +119,7 @@ workflow READSIMULATOR { // if ( params.amplicon ) { AMPLICON_WORKFLOW ( - ch_fasta.ifEmpty([]), + ch_fasta, ch_input ) ch_versions = ch_versions.mix(AMPLICON_WORKFLOW.out.versions.first()) @@ -118,7 +144,7 @@ workflow READSIMULATOR { // if ( params.metagenome ) { INSILICOSEQ_GENERATE ( - ch_input.combine(ch_fasta.ifEmpty([[]])) + ch_input.combine(ch_fasta) ) ch_versions = ch_versions.mix(INSILICOSEQ_GENERATE.out.versions.first()) ch_metagenome_reads = INSILICOSEQ_GENERATE.out.fastq From f7460a8f102c9c2d61471133e7eaa246649cc5c8 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 11 Jan 2024 02:44:36 +0000 Subject: [PATCH 61/89] Ran prettier --- nextflow_schema.json | 16 ++++++++++++++-- 1 file changed, 14 insertions(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 08ddcd2..408f552 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -365,13 +365,25 @@ "type": "string", "default": "all", "description": "The NCBI taxonomic groups to download. Options include 'all', 'archaea', 'bacteria', 'fungi', 'invertebrate', 'metagenomes', 'plant', 'protozoa', 'vertebrate_mammalian', 'vertebrate_other', and 'viral'. A comma-separated list is also valid (e.g., 'bacteria,viral').", - "enum": ["all", "archaea", "bacteria", "fungi", "invertebrate", "metagenomes", "plant", "protozoa", "vertebrate_mammalian", "vertebrate_other", "viral"] + "enum": [ + "all", + "archaea", + "bacteria", + "fungi", + "invertebrate", + "metagenomes", + "plant", + "protozoa", + "vertebrate_mammalian", + "vertebrate_other", + "viral" + ] }, "ncbidownload_section": { "type": "string", "default": "refseq", "description": "The NCBI section to download. 'refseq' or 'genbank'.", - "enum": ["refseq", "genbank"] + "enum": ["refseq", "genbank"] } } }, From 2dc52b1dd3010822cd55577f05a46338f23e715c Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 11 Jan 2024 02:49:57 +0000 Subject: [PATCH 62/89] removed crabs_dbdownload --- .../local/crabs/dbdownload/environment.yml | 7 ---- modules/local/crabs/dbdownload/main.nf | 42 ------------------- 2 files changed, 49 deletions(-) delete mode 100644 modules/local/crabs/dbdownload/environment.yml delete mode 100644 modules/local/crabs/dbdownload/main.nf diff --git a/modules/local/crabs/dbdownload/environment.yml b/modules/local/crabs/dbdownload/environment.yml deleted file mode 100644 index 8380980..0000000 --- a/modules/local/crabs/dbdownload/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: crabs_dbdownload -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::crabs=0.1.1-0 diff --git a/modules/local/crabs/dbdownload/main.nf b/modules/local/crabs/dbdownload/main.nf deleted file mode 100644 index 55414b0..0000000 --- a/modules/local/crabs/dbdownload/main.nf +++ /dev/null @@ -1,42 +0,0 @@ -process CRABS_DBDOWNLOAD { - - tag "" - label 'process_medium' - - // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/crabs:0.1.1--pyhb7b1952_0': - 'biocontainers/crabs:0.1.1--pyhb7b1952_0' }" - - input: - val amplicon_ncbi_db - val amplicon_embl_db - val amplicon_bold_db - - output: - path("*.fasta") , emit: fasta - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def input_args = amplicon_ncbi_db ? "--source ncbi --database ${params.amplicon_ncbi_db} --output ncbi.fa" : - amplicon_embl_db ? "--source embl --database ${params.amplicon_embl_db} --output embl.fa " : - amplicon_bold_db ? "--source bold --database ${params.amplicon_bold_db} --output bold.fa" : - "" - - def VERSION = '0.1.1' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. - """ - crabs db_download \\ - $args \\ - $input_args - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - crabs: $VERSION - END_VERSIONS - """ -} From a8d593876d3e4d4b17ca13382e33ccc45281a965 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 11 Jan 2024 03:38:53 +0000 Subject: [PATCH 63/89] fixed a few mistakes --- conf/modules.config | 17 +---------- conf/test_full.config | 4 +-- subworkflows/local/target_capture_workflow.nf | 30 +++++++++---------- 3 files changed, 18 insertions(+), 33 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 920f118..a74c908 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -62,20 +62,6 @@ process { ] } - withName: CRABS_DBDOWNLOAD { - ext.args = - params.amplicon_ref_source == "ncbi" ? - "--source ncbi --database ${params.amplicon_ncbi_db} --query '${params.amplicon_ncbi_query}' --email johndoe@gmail.com --batchsize ${params.amplicon_ncbi_batchsize} --keep_original no --output ncbi.fasta" : - params.amplicon_ref_source == "embl" ? - "--source embl --database ${params.amplicon_embl_db} --output embl.fasta --keep_original no" : - params.amplicon_ref_source == "bold" ? - "--source bold --database '${params.amplicon_bold_db}' --keep_original no --output bold.fasta" : "" - publishDir = [ - path: { "${params.outdir}/crabs_dbdownload" }, - mode: params.publish_dir_mode - ] - } - withName: CRABS_DBIMPORT { ext.args = "--seq_header species --delim ' '" publishDir = [ @@ -85,7 +71,7 @@ process { } withName: CRABS_INSILICOPCR { - ext.args = "--error 4.5 -t 12 --fwd ${params.fw_primer} --rev ${params.rv_primer}" + ext.args = "--error 4.5 -t 12 --fwd ${params.amplicon_fw_primer} --rev ${params.amplicon_rv_primer}" publishDir = [ path: { "${params.outdir}/crabs_insilicopcr" }, mode: params.publish_dir_mode @@ -121,7 +107,6 @@ process { params.metagenome_coverage ? "--coverage ${params.metagenome_coverage}" : "", params.metagenome_gc_bias ? "--gc_bias ${params.metagenome_gc_bias}" : "" ].join(' ').trim() - ext.args2 = "${params.metagenome_ncbi_db} --n_genomes_ncbi ${params.metagenome_n_genomes}" publishDir = [ path: { "${params.outdir}/insilicoseq" }, mode: params.publish_dir_mode diff --git a/conf/test_full.config b/conf/test_full.config index 63a0240..80d10e7 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -23,8 +23,8 @@ params { target_capture = true wholegenome = true - fw_primer = 'AAAATAAT' - rv_primer = 'GATTACTTT' + amplicon_fw_primer = 'AAAATAAT' + amplicon_rv_primer = 'GATTACTTT' amplicon_read_count = 1000 metagenome_n_reads = '100K' probe_ref_name = 'Diptera-2.7Kv1' diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index 53c0f63..011d9de 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -27,23 +27,23 @@ workflow TARGET_CAPTURE_WORKFLOW { ch_probes = UNZIP ( ch_zip_file ).file - } + } else { + // + // MODULE: Run bedtools_getfasta if the probe file is a bed file + // + if ( params.probe_file.endsWith('.bed') ) { + // Bedtools_getfasta requires an uncompressed fasta file + ch_uncompressed_fasta = UNCOMPRESS_FASTA ( + ch_fasta + ).fasta - // - // MODULE: Run bedtools_getfasta if the probe file is a bed file - // - if ( params.probe_file.endsWith('.bed') ) { - // Bedtools_getfasta requires an uncompressed fasta file - ch_uncompressed_fasta = UNCOMPRESS_FASTA ( - ch_fasta - ).fasta + BEDTOOLS_GETFASTA ( + ch_probes, + ch_uncompressed_fasta + ) - BEDTOOLS_GETFASTA ( - ch_probes, - ch_uncompressed_fasta - ) - - ch_probes = BEDTOOLS_GETFASTA.out.fasta + ch_probes = BEDTOOLS_GETFASTA.out.fasta + } } ch_probes = ch_probes From fc9eccc89369375f045ea3298e0d18a94d6028ba Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 11 Jan 2024 04:42:25 +0000 Subject: [PATCH 64/89] added citions for ncbi-genome-download and bedtools --- CITATIONS.md | 8 ++++++++ docs/output.md | 2 +- 2 files changed, 9 insertions(+), 1 deletion(-) diff --git a/CITATIONS.md b/CITATIONS.md index 1674452..06f6127 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -14,6 +14,10 @@ > Weichun Huang, Leping Li, Jason R. Myers, Gabor T. Marth, ART: a next-generation sequencing read simulator, Bioinformatics, Volume 28, Issue 4, February 2012, Pages 593–594, https://doi.org/10.1093/bioinformatics/btr708 +- [bedtools](https://pubmed.ncbi.nlm.nih.gov/20110278/) + + > Quinlan AR and Hall IM, 2010. BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics. 26, 6, pp. 841–842. hhtps://doi.org/10.1093/bioinformatics/btq033 + - [Bowtie2](https://www.nature.com/articles/nmeth.1923) > Langmead, B., Salzberg, S. Fast gapped-read alignment with Bowtie 2. Nat Methods 9, 357–359 (2012). https://doi.org/10.1038/nmeth.1923 @@ -38,6 +42,10 @@ > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. +- [ncbi-genome-download](https://zenodo.org/records/8192486) + + > DOI: 10.5281/zenodo.8192432 + - [Samtools](https://academic.oup.com/gigascience/article/10/2/giab008/6137722?login=false) > Twelve years of SAMtools and BCFtools. Petr Danecek, James K Bonfield, Jennifer Liddle, John Marshall, Valeriu Ohan, Martin O Pollard, Andrew Whitwham, Thomas Keane, Shane A McCarthy, Robert M Davies, Heng Li. GigaScience, Volume 10, Issue 2, February 2021, giab008, https://doi.org/10.1093/gigascience/giab008 diff --git a/docs/output.md b/docs/output.md index 29c2b54..c9dd419 100644 --- a/docs/output.md +++ b/docs/output.md @@ -18,7 +18,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [FastQC](#fastqc) - Raw read QC - [InSilicoSeq](#insilicoseq) - Simulated metagenomic reads - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline -- [ncbi-genome-download](#ncbigenomedownload) - Reference fasta files +- [ncbi-genome-download](#ncbi-genome-download) - Reference fasta files - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution - [Samplesheet](#samplesheet) - Samplesheets produced during the running of the pipeline - [Unzip](#unzip) - Unziped probe file From 500298d472e213d97c529d50096cb7108387d713 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 11 Jan 2024 04:50:25 +0000 Subject: [PATCH 65/89] updated documentation --- docs/output.md | 8 +++++--- 1 file changed, 5 insertions(+), 3 deletions(-) diff --git a/docs/output.md b/docs/output.md index c9dd419..eef32fa 100644 --- a/docs/output.md +++ b/docs/output.md @@ -35,6 +35,8 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
    +[ART](https://www.niehs.nih.gov/research/resources/software/biostatistics/art/index.cfm) is a tool for simulating Illumina sequencing reads. For further reading and documentation see the [ART Illumina manual](https://manpages.debian.org/testing/art-nextgen-simulation-tools/art_illumina.1.en.html). + ### bedtools
    @@ -45,7 +47,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
    -[ART](https://www.niehs.nih.gov/research/resources/software/biostatistics/art/index.cfm) is a tool for simulating Illumina sequencing reads. For further reading and documentation see the [ART Illumina manual](https://manpages.debian.org/testing/art-nextgen-simulation-tools/art_illumina.1.en.html). +[bedtools](https://pubmed.ncbi.nlm.nih.gov/20110278/) is a suite of tools for genomic data analysis. For further reading and documentation see the [bedtools documentation](https://bedtools.readthedocs.io/en/latest/). ### Bowtie2 @@ -85,8 +87,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
    Output files -- `crabs_dbdownload/` - - `*.fa`: Reference fasta file. - `crabs_dbimport/` - `*.fa`: Reference fasta file. - `crabs_insilicopcr/` @@ -129,6 +129,8 @@ The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They m
    +[ncbi-genome-download](https://github.com/kblin/ncbi-genome-download) downloads reference genome files from NCBI. + ### InSilicoSeq
    From f9fe6fe9254a211acadbcc582de364a70f863d98 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 11 Jan 2024 05:34:11 +0000 Subject: [PATCH 66/89] changed the name of UNZIP_PROBE to just UNZIP --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index a74c908..2b71267 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -160,7 +160,7 @@ process { ] } - withName: UNZIP_PROBE { + withName: UNZIP { publishDir = [ path: { "${params.outdir}/probes" }, mode: params.publish_dir_mode From 59885e334dc2a1cf54e8dcbfd1d2d22f024be3a3 Mon Sep 17 00:00:00 2001 From: Carson J Miller Date: Fri, 19 Jan 2024 15:49:19 +0000 Subject: [PATCH 67/89] Added workflow nf-tests --- .github/workflows/ci.yml | 111 ++- .gitignore | 2 + .nf-core.yml | 1 + conf/test.config | 8 +- conf/test_data.config | 764 ++++++++++++++++++ lib/NftestUtils.groovy | 38 + main.nf | 10 +- nf-test.config | 16 + subworkflows/local/amplicon_workflow.nf | 6 +- subworkflows/local/target_capture_workflow.nf | 2 +- .../main.nf} | 33 +- workflows/readsimulator/tests/tags.yml | 4 + .../readsimulator/tests/test_amplicon.nf.test | 40 + .../tests/test_amplicon.nf.test.snap | 101 +++ .../tests/test_metagenome.nf.test | 38 + .../tests/test_metagenome.nf.test.snap | 101 +++ .../tests/test_target_capture.nf.test | 38 + .../tests/test_target_capture.nf.test.snap | 101 +++ .../tests/test_wholegenome.nf.test | 37 + .../tests/test_wholegenome.nf.test.snap | 101 +++ 20 files changed, 1511 insertions(+), 41 deletions(-) create mode 100644 conf/test_data.config create mode 100644 lib/NftestUtils.groovy create mode 100644 nf-test.config rename workflows/{readsimulator.nf => readsimulator/main.nf} (86%) create mode 100644 workflows/readsimulator/tests/tags.yml create mode 100644 workflows/readsimulator/tests/test_amplicon.nf.test create mode 100644 workflows/readsimulator/tests/test_amplicon.nf.test.snap create mode 100644 workflows/readsimulator/tests/test_metagenome.nf.test create mode 100644 workflows/readsimulator/tests/test_metagenome.nf.test.snap create mode 100644 workflows/readsimulator/tests/test_target_capture.nf.test create mode 100644 workflows/readsimulator/tests/test_target_capture.nf.test.snap create mode 100644 workflows/readsimulator/tests/test_wholegenome.nf.test create mode 100644 workflows/readsimulator/tests/test_wholegenome.nf.test.snap diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 3590023..ec2de1f 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -1,31 +1,73 @@ -name: nf-core CI # This workflow runs the pipeline with the minimal test dataset to check that it completes without any syntax errors +name: nf-core CI on: - push: - branches: - - dev pull_request: release: types: [published] + merge_group: + types: + - checks_requested + branches: + - master + - dev env: NXF_ANSI_LOG: false + NFT_VER: "0.8.3" + NFT_WORKDIR: "~" + NFT_DIFF: "pdiff" + NFT_DIFF_ARGS: "--line-numbers --expand-tabs=2" concurrency: group: "${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}" cancel-in-progress: true jobs: + changes: + name: Check for changes + runs-on: ubuntu-latest + outputs: + # Expose matched filters as job 'tags' output variable + tags: ${{ steps.filter.outputs.changes }} + steps: + - uses: actions/checkout@v3 + - name: Combine all tags.yml files + id: get_username + run: find . -name "tags.yml" -not -path "./.github/*" -exec cat {} + > .github/tags.yml + - name: debug + run: cat .github/tags.yml + - uses: dorny/paths-filter@v2 + id: filter + with: + filters: ".github/tags.yml" + + define_nxf_versions: + name: Choose nextflow versions to test against depending on target branch + runs-on: ubuntu-latest + outputs: + matrix: ${{ steps.nxf_versions.outputs.matrix }} + steps: + - id: nxf_versions + run: | + if [[ "${{ github.event_name }}" == "pull_request" && "${{ github.base_ref }}" == "dev" && "${{ matrix.NXF_VER }}" != "latest-everything" ]]; then + echo matrix='["latest-everything"]' | tee -a $GITHUB_OUTPUT + else + echo matrix='["latest-everything", "23.04.0"]' | tee -a $GITHUB_OUTPUT + fi + test: - name: Run pipeline with test data - # Only run on push if this is the nf-core dev branch (merged PRs) - if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/readsimulator') }}" + name: ${{ matrix.tags }} ${{ matrix.profile }} NF ${{ matrix.NXF_VER }} + needs: [changes, define_nxf_versions] + if: needs.changes.outputs.tags != '[]' runs-on: ubuntu-latest strategy: + fail-fast: false matrix: - NXF_VER: - - "23.04.0" - - "latest-everything" + NXF_VER: ${{ fromJson(needs.define_nxf_versions.outputs.matrix) }} + tags: ["${{ fromJson(needs.changes.outputs.tags) }}"] + profile: + - "docker" + steps: - name: Check out pipeline code uses: actions/checkout@v4 @@ -35,9 +77,48 @@ jobs: with: version: "${{ matrix.NXF_VER }}" - - name: Run pipeline with test data - # TODO nf-core: You can customise CI pipeline run tests as required - # For example: adding multiple test runs with different parameters - # Remember that you can parallelise this by using strategy.matrix + - uses: actions/setup-python@v4 + with: + python-version: "3.11" + architecture: "x64" + + - name: Install pdiff to see diff between nf-test snapshots + run: | + python -m pip install --upgrade pip + pip install pdiff + + - name: Cache nf-test installation + id: cache-software + uses: actions/cache@v3 + with: + path: | + /usr/local/bin/nf-test + /home/runner/.nf-test/nf-test.jar + key: ${{ runner.os }}-${{ env.NFT_VER }}-nftest + + - name: Install nf-test + if: steps.cache-software.outputs.cache-hit != 'true' + run: | + wget -qO- https://code.askimed.com/install/nf-test | bash + sudo mv nf-test /usr/local/bin/ + + - name: Run nf-test run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results + nf-test test --verbose --tag ${{ matrix.tags }} --profile test,test_data,"${{ matrix.profile }}" --junitxml=test.xml --tap=test.tap + + - uses: pcolby/tap-summary@v1 + with: + path: >- + test.tap + + - name: Output log on failure + if: failure() + run: | + sudo apt install bat > /dev/null + batcat --decorations=always --color=always ${{ github.workspace }}/.nf-test/tests/*/meta/nextflow.log + + - name: Publish Test Report + uses: mikepenz/action-junit-report@v3 + if: always() # always run even if the previous step fails + with: + report_paths: test.xml diff --git a/.gitignore b/.gitignore index 5124c9a..8d38c7c 100644 --- a/.gitignore +++ b/.gitignore @@ -6,3 +6,5 @@ results/ testing/ testing* *.pyc +.nf-tests/ +.nf-test.log diff --git a/.nf-core.yml b/.nf-core.yml index 3805dc8..17ef22b 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1 +1,2 @@ repository_type: pipeline +actions_ci: false diff --git a/conf/test.config b/conf/test.config index dcb01e8..3a71f83 100644 --- a/conf/test.config +++ b/conf/test.config @@ -18,10 +18,6 @@ params { max_cpus = 2 max_memory = '6.GB' max_time = '6.h' - - // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/samplesheet/test_samplesheet.csv' - fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/testdata/GCF_024334085.1_ASM2433408v1_genomic.fna.gz' - - wholegenome = true } + +includeConfig './test_data.config' diff --git a/conf/test_data.config b/conf/test_data.config new file mode 100644 index 0000000..81ca3fd --- /dev/null +++ b/conf/test_data.config @@ -0,0 +1,764 @@ +// README: +// https://github.com/nf-core/test-datasets/blob/modules/README.md + +params { + // Base directory for test data + test_data_base = "https://raw.githubusercontent.com/nf-core/test-datasets/modules" + + test_data { + 'sarscov2' { + 'genome' { + genome_fasta = "${params.test_data_base}/data/genomics/sarscov2/genome/genome.fasta" + genome_fasta_gz = "${params.test_data_base}/data/genomics/sarscov2/genome/genome.fasta.gz" + genome_fasta_fai = "${params.test_data_base}/data/genomics/sarscov2/genome/genome.fasta.fai" + genome_fasta_txt_zst = "${params.test_data_base}/data/genomics/sarscov2/genome/genome.fasta.txt.zst" + genome_dict = "${params.test_data_base}/data/genomics/sarscov2/genome/genome.dict" + genome_gff3 = "${params.test_data_base}/data/genomics/sarscov2/genome/genome.gff3" + genome_gff3_gz = "${params.test_data_base}/data/genomics/sarscov2/genome/genome.gff3.gz" + genome_gtf = "${params.test_data_base}/data/genomics/sarscov2/genome/genome.gtf" + genome_paf = "${params.test_data_base}/data/genomics/sarscov2/genome/genome.paf" + genome_sizes = "${params.test_data_base}/data/genomics/sarscov2/genome/genome.sizes" + transcriptome_fasta = "${params.test_data_base}/data/genomics/sarscov2/genome/transcriptome.fasta" + transcriptome_paf = "${params.test_data_base}/data/genomics/sarscov2/genome/transcriptome.paf" + proteome_fasta = "${params.test_data_base}/data/genomics/sarscov2/genome/proteome.fasta" + proteome_fasta_gz = "${params.test_data_base}/data/genomics/sarscov2/genome/proteome.fasta.gz" + + test_bed = "${params.test_data_base}/data/genomics/sarscov2/genome/bed/test.bed" + test_bed_gz = "${params.test_data_base}/data/genomics/sarscov2/genome/bed/test.bed.gz" + test2_bed = "${params.test_data_base}/data/genomics/sarscov2/genome/bed/test2.bed" + test_bed12 = "${params.test_data_base}/data/genomics/sarscov2/genome/bed/test.bed12" + baits_bed = "${params.test_data_base}/data/genomics/sarscov2/genome/bed/baits.bed" + bed_autosql = "${params.test_data_base}/data/genomics/sarscov2/genome/bed/bed6alt.as" + + reference_cnn = "${params.test_data_base}/data/genomics/sarscov2/genome/cnn/reference.cnn" + + kraken2 = "${params.test_data_base}/data/genomics/sarscov2/genome/db/kraken2" + kraken2_tar_gz = "${params.test_data_base}/data/genomics/sarscov2/genome/db/kraken2.tar.gz" + + kraken2_bracken = "${params.test_data_base}/data/genomics/sarscov2/genome/db/kraken2_bracken" + kraken2_bracken_tar_gz = "${params.test_data_base}/data/genomics/sarscov2/genome/db/kraken2_bracken.tar.gz" + + kaiju = "${params.test_data_base}/data/genomics/sarscov2/genome/db/kaiju" + kaiju_tar_gz = "${params.test_data_base}/data/genomics/sarscov2/genome/db/kaiju.tar.gz" + + kofamscan_profiles_tar_gz = "${params.test_data_base}/data/genomics/sarscov2/genome/db/kofamscan/profiles.tar.gz" + kofamscan_ko_list_gz = "${params.test_data_base}/data/genomics/sarscov2/genome/db/kofamscan/ko_list.gz" + + ncbi_taxmap_zip = "${params.test_data_base}/data/genomics/sarscov2/genome/db/maltextract/ncbi_taxmap.zip" + taxon_list_txt = "${params.test_data_base}/data/genomics/sarscov2/genome/db/maltextract/taxon_list.txt" + + mmseqs_tar_gz = "${params.test_data_base}/data/genomics/sarscov2/genome/db/mmseqs.tar.gz" + + all_sites_fas = "${params.test_data_base}/data/genomics/sarscov2/genome/alignment/all_sites.fas" + informative_sites_fas = "${params.test_data_base}/data/genomics/sarscov2/genome/alignment/informative_sites.fas" + + contigs_genome_maf_gz = "${params.test_data_base}/data/genomics/sarscov2/genome/alignment/last/contigs.genome.maf.gz" + contigs_genome_par = "${params.test_data_base}/data/genomics/sarscov2/genome/alignment/last/contigs.genome.par" + lastdb_tar_gz = "${params.test_data_base}/data/genomics/sarscov2/genome/alignment/last/lastdb.tar.gz" + + baits_interval_list = "${params.test_data_base}/data/genomics/sarscov2/genome/picard/baits.interval_list" + targets_interval_list = "${params.test_data_base}/data/genomics/sarscov2/genome/picard/targets.interval_list" + regions_txt = "${params.test_data_base}/data/genomics/sarscov2/genome/graphtyper/regions.txt" + lc_extrap_mr = "${params.test_data_base}/data/delete_me/preseq/SRR1003759_5M_subset.mr" + } + 'illumina' { + test_single_end_bam = "${params.test_data_base}/data/genomics/sarscov2/illumina/bam/test.single_end.bam" + test_single_end_sorted_bam = "${params.test_data_base}/data/genomics/sarscov2/illumina/bam/test.single_end.sorted.bam" + test_single_end_sorted_bam_bai = "${params.test_data_base}/data/genomics/sarscov2/illumina/bam/test.single_end.sorted.bam.bai" + test_paired_end_bam = "${params.test_data_base}/data/genomics/sarscov2/illumina/bam/test.paired_end.bam" + test_paired_end_sorted_bam = "${params.test_data_base}/data/genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam" + test_paired_end_sorted_bam_bai = "${params.test_data_base}/data/genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai" + test_paired_end_methylated_bam = "${params.test_data_base}/data/genomics/sarscov2/illumina/bam/test.paired_end.methylated.bam" + test_paired_end_methylated_sorted_bam = "${params.test_data_base}/data/genomics/sarscov2/illumina/bam/test.paired_end.methylated.sorted.bam" + test_paired_end_methylated_sorted_bam_bai = "${params.test_data_base}/data/genomics/sarscov2/illumina/bam/test.paired_end.methylated.sorted.bam.bai" + test_unaligned_bam = "${params.test_data_base}/data/genomics/sarscov2/illumina/bam/test.unaligned.bam" + + test_1_fastq_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/fastq/test_1.fastq.gz" + test_2_fastq_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/fastq/test_2.fastq.gz" + test_interleaved_fastq_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz" + test_1_fastq_txt_zst = "${params.test_data_base}/data/genomics/sarscov2/illumina/fastq/test_1.fastq.txt.zst" + test2_1_fastq_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/fastq/test2_1.fastq.gz" + test2_2_fastq_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/fastq/test2_2.fastq.gz" + test_methylated_1_fastq_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/fastq/test.methylated_1.fastq.gz" + test_methylated_2_fastq_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/fastq/test.methylated_2.fastq.gz" + test_1_fastq_gz_fastqc_html = "${params.test_data_base}/data/genomics/sarscov2/illumina/fastqc/test_fastqc.html" + test_1_fastq_gz_fastqc_zip = "${params.test_data_base}/data/genomics/sarscov2/illumina/fastqc/test_fastqc.zip" + + test_bedgraph = "${params.test_data_base}/data/genomics/sarscov2/illumina/bedgraph/test.bedgraph" + + test_bigwig = "${params.test_data_base}/data/genomics/sarscov2/illumina/bigwig/test.bigwig" + + test_wig_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/wig/test.wig.gz" + + test_baserecalibrator_table = "${params.test_data_base}/data/genomics/sarscov2/illumina/gatk/test.baserecalibrator.table" + + test_computematrix_mat_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/deeptools/test.computeMatrix.mat.gz" + + test_bcf = "${params.test_data_base}/data/genomics/sarscov2/illumina/vcf/test.bcf" + + test_vcf = "${params.test_data_base}/data/genomics/sarscov2/illumina/vcf/test.vcf" + test_vcf_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/vcf/test.vcf.gz" + test_vcf_gz_tbi = "${params.test_data_base}/data/genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi" + test2_vcf = "${params.test_data_base}/data/genomics/sarscov2/illumina/vcf/test2.vcf" + test2_vcf_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/vcf/test2.vcf.gz" + test2_vcf_gz_tbi = "${params.test_data_base}/data/genomics/sarscov2/illumina/vcf/test2.vcf.gz.tbi" + test2_vcf_targets_tsv_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/vcf/test2.targets.tsv.gz" + test3_vcf = "${params.test_data_base}/data/genomics/sarscov2/illumina/vcf/test3.vcf" + test3_vcf_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/vcf/test3.vcf.gz" + test3_vcf_gz_tbi = "${params.test_data_base}/data/genomics/sarscov2/illumina/vcf/test3.vcf.gz.tbi" + + contigs_fasta = "${params.test_data_base}/data/genomics/sarscov2/illumina/fasta/contigs.fasta" + scaffolds_fasta = "${params.test_data_base}/data/genomics/sarscov2/illumina/fasta/scaffolds.fasta" + + assembly_gfa = "${params.test_data_base}/data/genomics/sarscov2/illumina/gfa/assembly.gfa" + assembly_gfa_bgz = "${params.test_data_base}/data/genomics/sarscov2/illumina/gfa/assembly.gfa.bgz" + assembly_gfa_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/gfa/assembly.gfa.gz" + assembly_gfa_zst = "${params.test_data_base}/data/genomics/sarscov2/illumina/gfa/assembly.gfa.zst" + + test_single_end_bam_readlist_txt = "${params.test_data_base}/data/genomics/sarscov2/illumina/picard/test.single_end.bam.readlist.txt" + + SRR13255544_tar_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/sra/SRR13255544.tar.gz" + SRR11140744_tar_gz = "${params.test_data_base}/data/genomics/sarscov2/illumina/sra/SRR11140744.tar.gz" + } + 'nanopore' { + test_sorted_bam = "${params.test_data_base}/data/genomics/sarscov2/nanopore/bam/test.sorted.bam" + test_sorted_bam_bai = "${params.test_data_base}/data/genomics/sarscov2/nanopore/bam/test.sorted.bam.bai" + + fast5_tar_gz = "${params.test_data_base}/data/genomics/sarscov2/nanopore/fast5/fast5.tar.gz" + + test_fastq_gz = "${params.test_data_base}/data/genomics/sarscov2/nanopore/fastq/test.fastq.gz" + + test_sequencing_summary = "${params.test_data_base}/data/genomics/sarscov2/nanopore/sequencing_summary/test.sequencing_summary.txt" + } + 'metagenome' { + classified_reads_assignment = "${params.test_data_base}/data/genomics/sarscov2/metagenome/test_1.kraken2.reads.txt" + kraken_report = "${params.test_data_base}/data/genomics/sarscov2/metagenome/test_1.kraken2.report.txt" + krona_taxonomy = "${params.test_data_base}/data/genomics/sarscov2/metagenome/krona_taxonomy.tab" + seqid2taxid_map = "${params.test_data_base}/data/genomics/sarscov2/metagenome/seqid2taxid.map" + nodes_dmp = "${params.test_data_base}/data/genomics/sarscov2/metagenome/nodes.dmp" + names_dmp = "${params.test_data_base}/data/genomics/sarscov2/metagenome/names.dmp" + prot_nodes_dmp = "${params.test_data_base}/data/genomics/sarscov2/metagenome/prot_nodes.dmp" + prot_names_dmp = "${params.test_data_base}/data/genomics/sarscov2/metagenome/prot_names.dmp" + prot_accession2taxid_gz = "${params.test_data_base}/data/genomics/sarscov2/metagenome/prot.accession2taxid.gz" + } + } + 'mus_musculus' { + 'genome' { + rnaseq_samplesheet = "${params.test_data_base}/data/genomics/mus_musculus/rnaseq_expression/SRP254919.samplesheet.csv" + rnaseq_genemeta = "${params.test_data_base}/data/genomics/mus_musculus/rnaseq_expression/SRP254919.gene_meta.tsv" + rnaseq_contrasts = "${params.test_data_base}/data/genomics/mus_musculus/rnaseq_expression/SRP254919.contrasts.csv" + rnaseq_matrix = "${params.test_data_base}/data/genomics/mus_musculus/rnaseq_expression/SRP254919.salmon.merged.gene_counts.top1000cov.tsv" + rnaseq_lengths = "${params.test_data_base}/data/genomics/mus_musculus/rnaseq_expression/SRP254919.spoofed_lengths.tsv" + deseq_results = "${params.test_data_base}/data/genomics/mus_musculus/rnaseq_expression/SRP254919.salmon.merged.deseq2.results.tsv" + genome_19_fasta = "${params.test_data_base}/data/genomics/mus_musculus/genome/chr19.fa.gz" + genome_19_gtf = "${params.test_data_base}/data/genomics/mus_musculus/genome/chr19.filtered.gtf.gz" + } + 'illumina' { + test_1_fastq_gz = "${params.test_data_base}/data/genomics/mus_musculus/mageck/ERR376998.small.fastq.gz" + test_2_fastq_gz = "${params.test_data_base}/data/genomics/mus_musculus/mageck/ERR376999.small.fastq.gz" + genome_config = "${params.test_data_base}/data/genomics/mus_musculus/illumina/10xgenomics/multiome/cellranger_arc_mkref_test_mm39_chr19_config.json" + multiome_lib_csv = "${params.test_data_base}/data/genomics/mus_musculus/illumina/10xgenomics/multiome/lib.csv" + test_scARC_gex_R1_fastq_gz = "${params.test_data_base}/data/genomics/mus_musculus/illumina/10xgenomics/multiome/SRR18907480_chr19_sub_S1_L001_R1_001.fastq.gz" + test_scARC_gex_R2_fastq_gz = "${params.test_data_base}/data/genomics/mus_musculus/illumina/10xgenomics/multiome/SRR18907480_chr19_sub_S1_L001_R2_001.fastq.gz" + test_scARC_atac_R1_fastq_gz = "${params.test_data_base}/data/genomics/mus_musculus/illumina/10xgenomics/multiome/SRR18907481_chr19_sub_S1_L001_R1_001.fastq.gz" + test_scARC_atac_R2_fastq_gz = "${params.test_data_base}/data/genomics/mus_musculus/illumina/10xgenomics/multiome/SRR18907481_chr19_sub_S1_L001_R2_001.fastq.gz" + test_scARC_atac_I2_fastq_gz = "${params.test_data_base}/data/genomics/mus_musculus/illumina/10xgenomics/multiome/SRR18907481_chr19_sub_S1_L001_I2_001.fastq.gz" + } + 'csv' { + count_table = "${params.test_data_base}/data/genomics/mus_musculus/mageck/count_table.csv" + library = "${params.test_data_base}/data/genomics/mus_musculus/mageck/yusa_library.csv" + } + 'txt' { + design_matrix = "${params.test_data_base}/data/genomics/mus_musculus/mageck/design_matrix.txt" + } + } + 'homo_sapiens' { + '10xgenomics' { + cellranger { + test_10x_10k_pbmc_5fb_fastq_1_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc/fastqs/5gex/5fb/subsampled_sc5p_v2_hs_PBMC_10k_5fb_S1_L001_R1_001.fastq.gz" + test_10x_10k_pbmc_5fb_fastq_2_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc/fastqs/5gex/5fb/subsampled_sc5p_v2_hs_PBMC_10k_5fb_S1_L001_R2_001.fastq.gz" + test_10x_10k_pbmc_5gex_fastq_1_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc/fastqs/5gex/5gex/subsampled_sc5p_v2_hs_PBMC_10k_5gex_S1_L001_R1_001.fastq.gz" + test_10x_10k_pbmc_5gex_fastq_2_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc/fastqs/5gex/5gex/subsampled_sc5p_v2_hs_PBMC_10k_5gex_S1_L001_R2_001.fastq.gz" + test_10x_10k_pbmc_b_fastq_1_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc/fastqs/bcell/subsampled_sc5p_v2_hs_PBMC_10k_b_S1_L001_R1_001.fastq.gz" + test_10x_10k_pbmc_b_fastq_2_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc/fastqs/bcell/subsampled_sc5p_v2_hs_PBMC_10k_b_S1_L001_R2_001.fastq.gz" + test_10x_10k_pbmc_t_fastq_1_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc/fastqs/tcell/subsampled_sc5p_v2_hs_PBMC_10k_t_S1_L001_R1_001.fastq.gz" + test_10x_10k_pbmc_t_fastq_2_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc/fastqs/tcell/subsampled_sc5p_v2_hs_PBMC_10k_t_S1_L001_R2_001.fastq.gz" + test_10x_10k_pbmc_feature_ref_csv = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc/sc5p_v2_hs_PBMC_10k_multi_5gex_5fb_b_t_feature_ref.csv" + + test_10x_10k_pbmc_cmo_cmo_fastq_1_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/cmo/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_multiplexing_capture_S1_L001_R1_001.fastq.gz" + test_10x_10k_pbmc_cmo_cmo_fastq_2_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/cmo/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_multiplexing_capture_S1_L001_R2_001.fastq.gz" + test_10x_10k_pbmc_cmo_gex1_fastq_1_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/gex_1/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R1_001.fastq.gz" + test_10x_10k_pbmc_cmo_gex1_fastq_2_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/gex_1/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R2_001.fastq.gz" + test_10x_10k_pbmc_cmo_gex2_fastq_1_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/gex_2/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_2_gex_S1_L001_R1_001.fastq.gz" + test_10x_10k_pbmc_cmo_gex2_fastq_2_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/gex_2/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_2_gex_S1_L001_R2_001.fastq.gz" + test_10x_10k_pbmc_cmo_feature_ref_csv = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/10k_pbmc_cmo_count_feature_reference.csv" + + test_10x_5k_cmvpos_tcells_ab_fastq_1_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/5k_cmvpos_tcells/fastqs/ab/subsampled_5k_human_antiCMV_T_TBNK_connect_AB_S2_L004_R1_001.fastq.gz" + test_10x_5k_cmvpos_tcells_ab_fastq_2_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/5k_cmvpos_tcells/fastqs/ab/subsampled_5k_human_antiCMV_T_TBNK_connect_AB_S2_L004_R2_001.fastq.gz" + test_10x_5k_cmvpos_tcells_gex1_fastq_1_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/5k_cmvpos_tcells/fastqs/gex_1/subsampled_5k_human_antiCMV_T_TBNK_connect_GEX_1_S1_L001_R1_001.fastq.gz" + test_10x_5k_cmvpos_tcells_gex1_fastq_2_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/5k_cmvpos_tcells/fastqs/gex_1/subsampled_5k_human_antiCMV_T_TBNK_connect_GEX_1_S1_L001_R2_001.fastq.gz" + test_10x_5k_cmvpos_tcells_vdj_fastq_1_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/5k_cmvpos_tcells/fastqs/vdj/subsampled_5k_human_antiCMV_T_TBNK_connect_VDJ_S1_L001_R1_001.fastq.gz" + test_10x_5k_cmvpos_tcells_vdj_fastq_2_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/5k_cmvpos_tcells/fastqs/vdj/subsampled_5k_human_antiCMV_T_TBNK_connect_VDJ_S1_L001_R2_001.fastq.gz" + test_10x_5k_cmvpos_tcells_feature_ref_csv = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/5k_cmvpos_tcells/5k_human_antiCMV_T_TBNK_connect_Multiplex_count_feature_reference.csv" + + test_10x_vdj_ref_json = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/references/vdj/refdata-cellranger-vdj-GRCh38-alts-ensembl-5.0.0/reference.json" + test_10x_vdj_ref_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/references/vdj/refdata-cellranger-vdj-GRCh38-alts-ensembl-5.0.0/fasta/regions.fa" + test_10x_vdj_ref_suppfasta = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger/references/vdj/refdata-cellranger-vdj-GRCh38-alts-ensembl-5.0.0/fasta/supp_regions.fa" + + test_scATAC_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger-atac/test_scATAC_S1_L001_R1_001.fastq.gz" + test_scATAC_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger-atac/test_scATAC_S1_L001_R2_001.fastq.gz" + test_scATAC_3_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger-atac/test_scATAC_S1_L001_R3_001.fastq.gz" + test_scATAC_I_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/cellranger-atac/test_scATAC_S1_L001_I1_001.fastq.gz" + } + spaceranger { + test_10x_ffpe_cytassist_fastq_1_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/spaceranger/human-brain-cancer-11-mm-capture-area-ffpe-2-standard_v2_ffpe_cytassist/CytAssist_11mm_FFPE_Human_Glioblastoma_2_S1_L001_R1_001.fastq.gz" + test_10x_ffpe_cytassist_fastq_2_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/spaceranger/human-brain-cancer-11-mm-capture-area-ffpe-2-standard_v2_ffpe_cytassist/CytAssist_11mm_FFPE_Human_Glioblastoma_2_S1_L001_R2_001.fastq.gz" + test_10x_ffpe_cytassist_image = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/spaceranger/human-brain-cancer-11-mm-capture-area-ffpe-2-standard_v2_ffpe_cytassist/CytAssist_11mm_FFPE_Human_Glioblastoma_image.tif" + test_10x_ffpe_cytassist_probeset = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/spaceranger/human-brain-cancer-11-mm-capture-area-ffpe-2-standard_v2_ffpe_cytassist/CytAssist_11mm_FFPE_Human_Glioblastoma_probe_set.csv" + + test_10x_ffpe_v1_fastq_1_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/spaceranger/human-ovarian-cancer-1-standard_v1_ffpe/Visium_FFPE_Human_Ovarian_Cancer_S1_L001_R1_001.fastq.gz" + test_10x_ffpe_v1_fastq_2_gz = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/spaceranger/human-ovarian-cancer-1-standard_v1_ffpe/Visium_FFPE_Human_Ovarian_Cancer_S1_L001_R2_001.fastq.gz" + test_10x_ffpe_v1_image = "${params.test_data_base}/data/genomics/homo_sapiens/10xgenomics/spaceranger/human-ovarian-cancer-1-standard_v1_ffpe/Visium_FFPE_Human_Ovarian_Cancer_image.jpg" + } + } + 'genome' { + genome_elfasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.elfasta" + genome_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta" + genome_fasta_fai = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta.fai" + genome_fasta_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta.gz" + genome_fasta_gz_fai = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta.gz.fai" + genome_fasta_gz_gzi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta.gz.gzi" + genome_strtablefile = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome_strtablefile.zip" + genome_dict = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.dict" + genome_gff3 = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.gff3" + genome_gtf = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.gtf" + genome_interval_list = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.interval_list" + genome_multi_interval_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.multi_intervals.bed" + genome_blacklist_interval_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.blacklist_intervals.bed" + genome_sizes = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.sizes" + genome_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.bed" + genome_header = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.header" + genome_bed_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.bed.gz" + genome_bed_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.bed.gz.tbi" + genome_elsites = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.elsites" + transcriptome_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/transcriptome.fasta" + genome2_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome2.fasta" + genome_chain_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.chain.gz" + genome_annotated_interval_tsv = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.annotated_intervals.tsv" + genome_mt_gb = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.NC_012920_1.gb" + genome_preprocessed_count_tsv = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.preprocessed_intervals.counts.tsv" + genome_preprocessed_interval_list = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.preprocessed_intervals.interval_list" + genome_ploidy_model = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.ploidy_model.tar.gz" + genome_ploidy_calls = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.ploidy_calls.tar.gz" + genome_germline_cnv_model = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.germline_cnv_model.tar.gz" + genome_germline_cnv_calls = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.germline_cnv_calls.tar.gz" + genome_motifs = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome_motifs.txt" + genome_config = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome_config.json" + + genome_1_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr1/genome.fasta.gz" + genome_1_gtf = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr1/genome.gtf" + + genome_21_sdf = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome_sdf.tar.gz" + genome_21_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.fasta" + genome_21_fasta_fai = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai" + genome_21_gencode_gtf = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/chr21_gencode.gtf" + genome_21_dict = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.dict" + genome_21_sizes = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.sizes" + genome_21_interval_list = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.interval_list" + genome_21_annotated_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/annotated.bed" + genome_21_multi_interval_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed" + genome_21_multi_interval_antitarget_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.antitarget.bed" + genome_21_multi_interval_bed_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed.gz" + genome_21_multi_interval_bed_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed.gz.tbi" + genome_21_chromosomes_dir = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/chromosomes.tar.gz" + genome_21_reference_cnn = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/reference_chr21.cnn" + genome_21_eigenstrat_snp = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/chr_21.snp" + genome_21_stitch_posfile = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/dbsnp_138.hg38.first_10_biallelic_sites.tsv" + + dbsnp_146_hg38_elsites = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.elsites" + dbsnp_146_hg38_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz" + dbsnp_146_hg38_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz.tbi" + gnomad_r2_1_1_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1.vcf.gz" + gnomad_r2_1_1_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1.vcf.gz.tbi" + mills_and_1000g_indels_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz" + mills_and_1000g_indels_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi" + syntheticvcf_short_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/syntheticvcf_short.vcf.gz" + syntheticvcf_short_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/syntheticvcf_short.vcf.gz.tbi" + syntheticvcf_short_score = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/syntheticvcf_short.score" + gnomad_r2_1_1_sv_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1-sv.vcf.gz" + gnomad2_r2_1_1_sv_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/gnomAD2.r2.1.1-sv.vcf.gz" + + hapmap_3_3_hg38_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/hapmap_3.3.hg38.vcf.gz" + hapmap_3_3_hg38_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/hapmap_3.3.hg38.vcf.gz.tbi" + res_1000g_omni2_5_hg38_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/1000G_omni2.5.hg38.vcf.gz" + res_1000g_omni2_5_hg38_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/1000G_omni2.5.hg38.vcf.gz.tbi" + res_1000g_phase1_snps_hg38_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/1000G_phase1.snps.hg38.vcf.gz" + res_1000g_phase1_snps_hg38_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/1000G_phase1.snps.hg38.vcf.gz.tbi" + dbsnp_138_hg38_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/dbsnp_138.hg38.vcf.gz" + dbsnp_138_hg38_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/dbsnp_138.hg38.vcf.gz.tbi" + gnomad_r2_1_1_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/gnomAD.r2.1.1.vcf.gz" + gnomad_r2_1_1_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/gnomAD.r2.1.1.vcf.gz.tbi" + mills_and_1000g_indels_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/mills_and_1000G.indels.hg38.vcf.gz" + mills_and_1000g_indels_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/mills_and_1000G.indels.hg38.vcf.gz.tbi" + haplotype_map = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/haplotype_map.txt" + dbNSFP_4_1a_21_hg38_txt_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/dbNSFP4.1a.21.txt.gz" + dbNSFP_4_1a_21_hg38_txt_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/dbNSFP4.1a.21.txt.gz.tbi" + ngscheckmate_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/SNP_GRCh38_hg38_wChr.bed" + + index_salmon = "${params.test_data_base}/data/genomics/homo_sapiens/genome/index/salmon" + repeat_expansions = "${params.test_data_base}/data/genomics/homo_sapiens/genome/loci/repeat_expansions.json" + justhusky_ped = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/ped/justhusky.ped" + justhusky_minimal_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/ped/justhusky_minimal.vcf.gz" + justhusky_minimal_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/ped/justhusky_minimal.vcf.gz.tbi" + + vcfanno_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/vcfanno/vcfanno_grch38_module_test.tar.gz" + vcfanno_toml = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/vcfanno/vcfanno.toml" + updsites_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/updsites.bed" + + prg_input = "${params.test_data_base}/data/genomics/homo_sapiens/genome/PRG_test.zip" + crispr_functional_counts = "${params.test_data_base}/data/genomics/homo_sapiens/genome/tsv/functional_genomics_counts.tsv" + crispr_functional_library = "${params.test_data_base}/data/genomics/homo_sapiens/genome/tsv/library_functional_genomics.tsv" + + vep_cache = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vep.tar.gz" + affy_array_samplesheet = "${params.test_data_base}/data/genomics/homo_sapiens/array_expression/GSE38751.csv" + affy_array_celfiles_tar = "${params.test_data_base}/data/genomics/homo_sapiens/array_expression/GSE38751_RAW.tar" + + } + 'pangenome' { + pangenome_fa = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.fa" + pangenome_fa_bgzip = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.fa.gz" + pangenome_fa_bgzip_fai = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.fa.gz.fai" + pangenome_fa_bgzip_gzi = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.fa.gz.gzi" + pangenome_paf = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.paf" + pangenome_paf_gz = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.paf.gz" + pangenome_panacus_tsv = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.panacus.tsv" + pangenome_seqwish_gfa = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.seqwish.gfa" + pangenome_smoothxg_gfa = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.smoothxg.gfa" + pangenome_gfaffix_gfa = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.gfaffix.gfa" + 'odgi' { + pangenome_og = "${params.test_data_base}/data/pangenomics/homo_sapiens/odgi/pangenome.og" + pangenome_lay = "${params.test_data_base}/data/pangenomics/homo_sapiens/odgi/pangenome.lay" + } + } + 'illumina' { + test_paired_end_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam" + test_paired_end_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai" + test_paired_end_name_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.name.sorted.bam" + test_paired_end_markduplicates_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam" + test_paired_end_markduplicates_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam.bai" + test_paired_end_markduplicates_sorted_referencesn_txt = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.referencesn.txt" + test_paired_end_recalibrated_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.recalibrated.sorted.bam" + test_paired_end_recalibrated_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.recalibrated.sorted.bam.bai" + test_paired_end_umi_consensus_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_consensus.bam" + test_paired_end_umi_converted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_converted.bam" + test_paired_end_umi_grouped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_grouped.bam" + test_paired_end_umi_histogram_txt = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_histogram.txt" + test_paired_end_umi_unsorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_unsorted.bam" + test_paired_end_umi_unsorted_tagged_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.unsorted_tagged.bam" + test_paired_end_hla = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/example_hla_pe.bam" + test_paired_end_hla_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/example_hla_pe.sorted.bam" + test_paired_end_hla_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/example_hla_pe.sorted.bam.bai" + test_rna_paired_end_sorted_chr6_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.rna.paired_end.sorted.chr6.bam" + test_rna_paired_end_sorted_chr6_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.rna.paired_end.sorted.chr6.bam.bai" + + test2_paired_end_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam" + test2_paired_end_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam.bai" + test2_paired_end_name_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.name.sorted.bam" + test2_paired_end_markduplicates_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.markduplicates.sorted.bam" + test2_paired_end_markduplicates_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.markduplicates.sorted.bam.bai" + test2_paired_end_recalibrated_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.recalibrated.sorted.bam" + test2_paired_end_recalibrated_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.recalibrated.sorted.bam.bai" + test2_paired_end_umi_consensus_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_consensus.bam" + test2_paired_end_umi_converted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_converted.bam" + test2_paired_end_umi_grouped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_grouped.bam" + test2_paired_end_umi_histogram_txt = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_histogram.txt" + test2_paired_end_umi_unsorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_unsorted.bam" + test2_paired_end_umi_unsorted_tagged_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.unsorted_tagged.bam" + test_paired_end_duplex_umi_unmapped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_unmapped.bam" + test_paired_end_duplex_umi_mapped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_mapped.bam" + test_paired_end_duplex_umi_mapped_tagged_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_mapped_tagged.bam" + test_paired_end_duplex_umi_grouped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_grouped.bam" + test_paired_end_duplex_umi_duplex_consensus_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_duplex_consensus.bam" + + mitochon_standin_recalibrated_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/mitochon_standin.recalibrated.sorted.bam" + mitochon_standin_recalibrated_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/mitochon_standin.recalibrated.sorted.bam.bai" + test_illumina_mt_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test_illumina_mt.bam" + test_illumina_mt_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test_illumina_mt.bam.bai" + + test3_single_end_markduplicates_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam" + + read_group_settings_txt = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/read_group_settings.txt" + + test_paired_end_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram" + test_paired_end_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai" + test_paired_end_markduplicates_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.markduplicates.sorted.cram" + test_paired_end_markduplicates_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.markduplicates.sorted.cram.crai" + test_paired_end_recalibrated_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram" + test_paired_end_recalibrated_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai" + + test2_paired_end_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.sorted.cram" + test2_paired_end_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.sorted.cram.crai" + test2_paired_end_markduplicates_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.markduplicates.sorted.cram" + test2_paired_end_markduplicates_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.markduplicates.sorted.cram.crai" + test2_paired_end_recalibrated_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.recalibrated.sorted.cram" + test2_paired_end_recalibrated_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.recalibrated.sorted.cram.crai" + test3_paired_end_recalibrated_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test3.paired_end.recalibrated.sorted.cram" + test3_paired_end_recalibrated_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test3.paired_end.recalibrated.sorted.cram.crai" + + test_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz" + test_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_2.fastq.gz" + test_umi_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test.umi_1.fastq.gz" + test_umi_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test.umi_2.fastq.gz" + test_airrseq_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_airrseq_umi_R1.fastq.gz" + test_airrseq_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_airrseq_R2.fastq.gz" + test2_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test2_1.fastq.gz" + test2_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test2_2.fastq.gz" + test2_umi_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test2.umi_1.fastq.gz" + test2_umi_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test2.umi_2.fastq.gz" + test_rnaseq_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz" + test_rnaseq_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz" + test_paired_end_duplex_umi_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_duplex_umi_1.fastq.gz" + test_paired_end_duplex_umi_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_duplex_umi_2.fastq.gz" + + test_baserecalibrator_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test.baserecalibrator.table" + test2_baserecalibrator_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test2.baserecalibrator.table" + test_pileups_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test.pileups.table" + test2_pileups_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test2.pileups.table" + + test_paired_end_sorted_dragstrmodel = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_paired_end_sorted_dragstrmodel.txt" + + test_genomicsdb_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_genomicsdb.tar.gz" + test_pon_genomicsdb_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_pon_genomicsdb.tar.gz" + + test2_haplotc_ann_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.ann.vcf.gz" + test2_haplotc_ann_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.ann.vcf.gz.tbi" + test_haplotc_cnn_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test_haplotcaller.cnn.vcf.gz" + test_haplotc_cnn_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test_haplotcaller.cnn.vcf.gz.tbi" + + test2_haplotc_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz" + test2_haplotc_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz.tbi" + + test2_recal = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2.recal" + test2_recal_idx = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2.recal.idx" + test2_tranches = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2.tranches" + test2_allele_specific_recal = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2_allele_specific.recal" + test2_allele_specific_recal_idx = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2_allele_specific.recal.idx" + test2_allele_specific_tranches = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2_allele_specific.tranches" + + test_test2_paired_mutect2_calls_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz" + test_test2_paired_mutect2_calls_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz.tbi" + test_test2_paired_mutect2_calls_vcf_gz_stats = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz.stats" + test_test2_paired_mutect2_calls_f1r2_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.f1r2.tar.gz" + test_test2_paired_mutect2_calls_artifact_prior_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_test2_paired_mutect2_calls.artifact-prior.tar.gz" + test_test2_paired_segmentation_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_test2_paired.segmentation.table" + test_test2_paired_contamination_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_test2_paired.contamination.table" + + test_genome_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test.genome.vcf" + test_genome_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test.genome.vcf.gz" + test_genome_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test.genome.vcf.gz.tbi" + test_genome_vcf_idx = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test.genome.vcf.idx" + + test_genome_vcf_ud = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/svd/test.genome.vcf.UD" + test_genome_vcf_mu = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/svd/test.genome.vcf.mu" + test_genome_vcf_bed = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/svd/test.genome.vcf.bed" + + test2_genome_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test2.genome.vcf" + test2_genome_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test2.genome.vcf.gz" + test2_genome_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test2.genome.vcf.gz.tbi" + test2_genome_vcf_idx = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test2.genome.vcf.idx" + + test_genome21_indels_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test.genome_21.somatic_sv.vcf.gz" + test_genome21_indels_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test.genome_21.somatic_sv.vcf.gz.tbi" + + test_mpileup = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/mpileup/test.mpileup.gz" + test2_mpileup = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/mpileup/test2.mpileup.gz" + + test_broadpeak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/broadpeak/test.broadPeak" + test2_broadpeak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/broadpeak/test2.broadPeak" + + test_narrowpeak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/narrowpeak/test.narrowPeak" + test2_narrowpeak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/narrowpeak/test2.narrowPeak" + + test_yak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/yak/test.yak" + test2_yak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/yak/test2.yak" + + cutandrun_bedgraph_test_1 = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bedgraph/cutandtag_h3k27me3_test_1.bedGraph" + cutandrun_bedgraph_test_2 = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bedgraph/cutandtag_igg_test_1.bedGraph" + na24385_chr22_coverage = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bedgraph/NA24385_coverage.bed" + + empty_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/empty.vcf.gz" + empty_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/empty.vcf.gz.tbi" + + simulated_sv = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/chr21/simulated_sv.vcf.gz" + simulated_sv_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/chr21/simulated_sv.vcf.gz.tbi" + simulated_sv2 = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/chr21/simulated_sv2.vcf.gz" + simulated_sv2_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/chr21/simulated_sv2.vcf.gz.tbi" + + test_rnaseq_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test.rnaseq.vcf" + test_sv_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/sv_query.vcf.gz" + test_sv_vcf_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/sv_query.vcf.gz.tbi" + na24385_chr22_sv_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/NA24385_sv.vcf.gz" + na24385_chr22_sv_vcf_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/NA24385_sv.vcf.gz.tbi" + genmod_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/genmod.vcf.gz" + genmod_annotate_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test_annotate.vcf.gz" + genmod_models_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test_models.vcf.gz" + genmod_score_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test_score.vcf.gz" + + test_mito_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/NA12878_chrM.vcf.gz" + + test_pytor = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/pytor/test.pytor" + rank_model = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/genmod/svrank_model_-v1.8-.ini" + + test_flowcell = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bcl/flowcell.tar.gz" + test_flowcell_samplesheet = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bcl/flowcell_samplesheet.csv" + + varlociraptor_scenario = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/varlociraptor/scenario.yml" + + contig_ploidy_priors_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/contig_ploidy_priors_table.tsv" + + purecn_ex1_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex1.bam" + purecn_ex1_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex1.bam.bai" + purecn_ex1_interval = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex1_intervals.txt" + purecn_ex1_normal = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex1_normal.txt.gz" + purecn_ex2_normal = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex2_normal.txt.gz" + purecn_normalpanel_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_normalpanel.vcf.gz" + purecn_normalpanel_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_normalpanel.vcf.gz.tbi" + } + 'pacbio' { + primers = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/fasta/primers.fasta" + alz = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/bam/alz.bam" + alzpbi = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/bam/alz.bam.pbi" + ccs = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/bam/alz.ccs.bam" + ccs_fa = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/fasta/alz.ccs.fasta" + ccs_fa_gz = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/fasta/alz.ccs.fasta.gz" + ccs_fq = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/fastq/alz.ccs.fastq" + ccs_fq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/fastq/alz.ccs.fastq.gz" + ccs_xml = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/xml/alz.ccs.consensusreadset.xml" + hifi = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/fastq/test_hifi.fastq.gz" + lima = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/bam/alz.ccs.fl.NEB_5p--NEB_Clontech_3p.bam" + refine = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/bam/alz.ccs.fl.NEB_5p--NEB_Clontech_3p.flnc.bam" + cluster = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/bam/alz.ccs.fl.NEB_5p--NEB_Clontech_3p.flnc.clustered.bam" + singletons = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/bam/alz.ccs.fl.NEB_5p--NEB_Clontech_3p.flnc.clustered.singletons.bam" + aligned = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/bam/alz.ccs.fl.NEB_5p--NEB_Clontech_3p.flnc.clustered.singletons.merged.aligned.bam" + alignedbai = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/bam/alz.ccs.fl.NEB_5p--NEB_Clontech_3p.flnc.clustered.singletons.merged.aligned.bam.bai" + genemodel1 = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/bed/alz.ccs.fl.NEB_5p--NEB_Clontech_3p.flnc.clustered.singletons.merged.aligned_tc.bed" + genemodel2 = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/bed/alz.ccs.fl.NEB_5p--NEB_Clontech_3p.flnc.clustered.singletons.merged.aligned_tc.2.bed" + filelist = "${params.test_data_base}/data/genomics/homo_sapiens/pacbio/txt/filelist.txt" + } + 'scramble' { + fasta = "${params.test_data_base}/data/genomics/homo_sapiens/scramble/test.fa" + fasta_fai = "${params.test_data_base}/data/genomics/homo_sapiens/scramble/test.fa.fai" + bam = "${params.test_data_base}/data/genomics/homo_sapiens/scramble/test.bam" + bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/scramble/test.bam.bai" + cram = "${params.test_data_base}/data/genomics/homo_sapiens/scramble/test.cram" + cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/scramble/test.cram.crai" + bed = "${params.test_data_base}/data/genomics/homo_sapiens/scramble/test.bed" + } + 'gene_set_analysis' { + gct = "${params.test_data_base}/data/genomics/homo_sapiens/gene_set_analysis/P53_6samples_collapsed_symbols.gct" + cls = "${params.test_data_base}/data/genomics/homo_sapiens/gene_set_analysis/P53_6samples.cls" + gmx = "${params.test_data_base}/data/genomics/homo_sapiens/gene_set_analysis/c1.symbols.reduced.gmx" + } + 'cnvkit' { + amplicon_cnr = "https://raw.githubusercontent.com/etal/cnvkit/v0.9.9/test/formats/amplicon.cnr" + amplicon_cns = "https://raw.githubusercontent.com/etal/cnvkit/v0.9.9/test/formats/amplicon.cns" + } + } + 'bacteroides_fragilis' { + 'genome' { + genome_fna_gz = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/genome/genome.fna.gz" + genome_gbff_gz = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/genome/genome.gbff.gz" + genome_paf = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/genome/genome.paf" + genome_gff_gz = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/genome/genome.gff.gz" + + } + 'hamronization' { + genome_abricate_tsv = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/hamronization/genome.abricate.tsv" + genome_mapping_potential_arg = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/hamronization/genome.mapping.potential.ARG" + } + 'illumina' { + test1_contigs_fa_gz = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/illumina/fasta/test1.contigs.fa.gz" + test1_1_fastq_gz = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/illumina/fastq/test1_1.fastq.gz" + test1_2_fastq_gz = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/illumina/fastq/test1_2.fastq.gz" + test2_1_fastq_gz = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/illumina/fastq/test2_1.fastq.gz" + test2_2_fastq_gz = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/illumina/fastq/test2_2.fastq.gz" + test1_paired_end_bam = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/illumina/bam/test1.bam" + test1_paired_end_sorted_bam = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/illumina/bam/test1.sorted.bam" + test1_paired_end_sorted_bam_bai = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/illumina/bam/test1.sorted.bam.bai" + test2_paired_end_bam = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/illumina/bam/test2.bam" + test2_paired_end_sorted_bam = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/illumina/bam/test2.sorted.bam" + test2_paired_end_sorted_bam_bai = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/illumina/bam/test2.sorted.bam.bai" + } + 'nanopore' { + test_fastq_gz = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/nanopore/fastq/test.fastq.gz" + overlap_paf = "${params.test_data_base}/data/genomics/prokaryotes/bacteroides_fragilis/nanopore/overlap.paf" + } + } + 'candidatus_portiera_aleyrodidarum' { + 'genome' { + genome_fasta = "${params.test_data_base}/data/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/genome/genome.fasta" + genome_sizes = "${params.test_data_base}/data/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/genome/genome.sizes" + genome_aln_gz = "${params.test_data_base}/data/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/genome/genome.aln.gz" + genome_aln_nwk = "${params.test_data_base}/data/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/genome/genome.aln.nwk" + proteome_fasta = "${params.test_data_base}/data/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/genome/proteome.fasta" + test1_gff = "${params.test_data_base}/data/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/genome/gff/test1.gff" + test2_gff = "${params.test_data_base}/data/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/genome/gff/test2.gff" + test3_gff = "${params.test_data_base}/data/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/genome/gff/test3.gff" + } + 'illumina' { + test_1_fastq_gz = "${params.test_data_base}/data/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/illumina/fastq/test_1.fastq.gz" + test_2_fastq_gz = "${params.test_data_base}/data/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/illumina/fastq/test_2.fastq.gz" + test_se_fastq_gz = "${params.test_data_base}/data/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/illumina/fastq/test_se.fastq.gz" + } + 'nanopore' { + test_fastq_gz = "${params.test_data_base}/data/genomics/prokaryotes/candidatus_portiera_aleyrodidarum/nanopore/fastq/test.fastq.gz" + } + } + 'haemophilus_influenzae' { + 'genome' { + genome_fna_gz = "${params.test_data_base}/data/genomics/prokaryotes/haemophilus_influenzae/genome/genome.fna.gz" + genome_aln_gz = "${params.test_data_base}/data/genomics/prokaryotes/haemophilus_influenzae/genome/genome.aln.gz" + genome_aln_nwk = "${params.test_data_base}/data/genomics/prokaryotes/haemophilus_influenzae/genome/genome.aln.nwk" + } + } + 'generic' { + 'csv' { + test_csv = "${params.test_data_base}/data/generic/csv/test.csv" + } + 'notebooks' { + rmarkdown = "${params.test_data_base}/data/generic/notebooks/rmarkdown/rmarkdown_notebook.Rmd" + ipython_md = "${params.test_data_base}/data/generic/notebooks/jupyter/ipython_notebook.md" + ipython_ipynb = "${params.test_data_base}/data/generic/notebooks/jupyter/ipython_notebook.ipynb" + } + 'tar' { + tar_gz = "${params.test_data_base}/data/generic/tar/hello.tar.gz" + } + 'tsv' { + test_tsv = "${params.test_data_base}/data/generic/tsv/test.tsv" + } + 'txt' { + hello = "${params.test_data_base}/data/generic/txt/hello.txt" + } + 'cooler'{ + test_pairix_pair_gz = "${params.test_data_base}/data/genomics/homo_sapiens/cooler/cload/hg19/hg19.GM12878-MboI.pairs.subsample.blksrt.txt.gz" + test_pairix_pair_gz_px2 = "${params.test_data_base}/data/genomics/homo_sapiens/cooler/cload/hg19/hg19.GM12878-MboI.pairs.subsample.blksrt.txt.gz.px2" + test_pairs_pair = "${params.test_data_base}/data/genomics/homo_sapiens/cooler/cload/hg19/hg19.sample1.pairs" + test_tabix_pair_gz = "${params.test_data_base}/data/genomics/homo_sapiens/cooler/cload/hg19/hg19.GM12878-MboI.pairs.subsample.sorted.possrt.txt.gz" + test_tabix_pair_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/cooler/cload/hg19/hg19.GM12878-MboI.pairs.subsample.sorted.possrt.txt.gz.tbi" + hg19_chrom_sizes = "${params.test_data_base}/data/genomics/homo_sapiens/cooler/cload/hg19/hg19.chrom.sizes" + test_merge_cool = "${params.test_data_base}/data/genomics/homo_sapiens/cooler/merge/toy/toy.symm.upper.2.cool" + test_merge_cool_cp2 = "${params.test_data_base}/data/genomics/homo_sapiens/cooler/merge/toy/toy.symm.upper.2.cp2.cool" + + } + 'pairtools' { + mock_4dedup_pairsam = "${params.test_data_base}/data/genomics/homo_sapiens/pairtools/mock.4dedup.pairsam" + mock_4flip_pairs = "${params.test_data_base}/data/genomics/homo_sapiens/pairtools/mock.4flip.pairs" + mock_chrom_sizes = "${params.test_data_base}/data/genomics/homo_sapiens/pairtools/mock.chrom.sizes" + mock_pairsam = "${params.test_data_base}/data/genomics/homo_sapiens/pairtools/mock.pairsam" + mock_sam = "${params.test_data_base}/data/genomics/homo_sapiens/pairtools/mock.sam" + frag_bed = "${params.test_data_base}/data/genomics/homo_sapiens/pairtools/frag.bed" + } + 'estsfs'{ + config_file = "${params.test_data_base}/data/delete_me/estsfs/config-JC.txt" + data_file = "${params.test_data_base}/data/delete_me/estsfs/TEST-DATA.TXT" + seed_file = "${params.test_data_base}/data/delete_me/estsfs/seedfile.txt" + } + 'config' { + ncbi_user_settings = "${params.test_data_base}/data/generic/config/ncbi_user_settings.mkfg" + } + 'unsorted_data' { + 'unsorted_text' { + genome_file = "${params.test_data_base}/data/generic/unsorted_data/unsorted_text/test.genome" + intervals = "${params.test_data_base}/data/generic/unsorted_data/unsorted_text/test.bed" + numbers_csv = "${params.test_data_base}/data/generic/unsorted_data/unsorted_text/test.csv" + } + } + } + 'proteomics' { + 'msspectra' { + ups_file1 = "${params.test_data_base}/data/proteomics/msspectra/OVEMB150205_12.raw" + ups_file2 = "${params.test_data_base}/data/proteomics/msspectra/OVEMB150205_14.raw" + profile_spectra = "${params.test_data_base}/data/proteomics/msspectra/peakpicker_tutorial_1.mzML" + } + 'database' { + yeast_ups = "${params.test_data_base}/data/proteomics/database/yeast_UPS.fasta" + } + 'maxquant' { + mq_contrasts = "${params.test_data_base}/data/proteomics/maxquant/MaxQuant_contrasts.csv" + mq_proteingroups = "${params.test_data_base}/data/proteomics/maxquant/MaxQuant_proteinGroups.txt" + mq_samplesheet = "${params.test_data_base}/data/proteomics/maxquant/MaxQuant_samplesheet.tsv" + mq_proteus_mat = "${params.test_data_base}/data/proteomics/maxquant/proteus.raw_MaxQuant_proteingroups_tab.tsv" + } + 'parameter' { + maxquant = "${params.test_data_base}/data/proteomics/parameter/mqpar.xml" + } + 'openms' { + idxml1 = "${params.test_data_base}/data/proteomics/openms/HepG2_rep1_small.idXML" + idxml2 = "${params.test_data_base}/data/proteomics/openms/HepG2_rep2_small.idXML" + } + 'pdb' { + tim1_pdb = "${params.test_data_base}/data/proteomics/pdb/1tim.pdb" + tim8_pdb = "${params.test_data_base}/data/proteomics/pdb/8tim.pdb" + } + } + 'galaxea_fascicularis' { + hic { + pretext = "${params.test_data_base}/data/genomics/eukaryotes/galaxea_fascicularis/hic/jaGalFasc40_2.pretext" + } + } + 'deilephila_porcellus' { + 'mito' { + ref_fa = "${params.test_data_base}/data/genomics/eukaryotes/deilephila_porcellus/mito/MW539688.1.fasta" + ref_gb = "${params.test_data_base}/data/genomics/eukaryotes/deilephila_porcellus/mito/MW539688.1.gb" + hifi_reads = "${params.test_data_base}/data/genomics/eukaryotes/deilephila_porcellus/mito/ilDeiPorc1.HiFi.reads.fa" + contigs = "${params.test_data_base}/data/genomics/eukaryotes/deilephila_porcellus/mito/ilDeiPorc1.contigs.fa" + } + } + 'imaging' { + 'h5' { + plant_wga = "${params.test_data_base}/data/imaging/h5/plant_wga.h5" + plant_wga_prob = "${params.test_data_base}/data/imaging/h5/plant_wga_probabilities.h5" + } + 'ilp' { + plant_wga_multicut = "${params.test_data_base}/data/imaging/ilp/plant_wga.multicut.ilp" + plant_wga_pixel_class = "${params.test_data_base}/data/imaging/ilp/plant_wga.pixel_prob.ilp" + } + 'tiff' { + mouse_heart_wga = "${params.test_data_base}/data/imaging/tiff/mindagap.mouse_heart.wga.tiff" + } + 'ome-tiff' { + cycif_tonsil_channels = "${params.test_data_base}/data/imaging/ome-tiff/cycif-tonsil-channels.csv" + cycif_tonsil_cycle1 = "${params.test_data_base}/data/imaging/ome-tiff/cycif-tonsil-cycle1.ome.tif" + cycif_tonsil_cycle2 = "${params.test_data_base}/data/imaging/ome-tiff/cycif-tonsil-cycle2.ome.tif" + cycif_tonsil_cycle3 = "${params.test_data_base}/data/imaging/ome-tiff/cycif-tonsil-cycle3.ome.tif" + cycif_tonsil_dfp = "${params.test_data_base}/data/imaging/ome-tiff/cycif-tonsil-dfp.ome.tif" + cycif_tonsil_ffp = "${params.test_data_base}/data/imaging/ome-tiff/cycif-tonsil-ffp.ome.tif" + } + 'registration' { + markers = "${params.test_data_base}/data/imaging/registration/markers.csv" + cycle1 = "${params.test_data_base}/data/imaging/ome-tiff/cycif-tonsil-cycle1.ome.tif" + cycle2 = "${params.test_data_base}/data/imaging/ome-tiff/cycif-tonsil-cycle2.ome.tif" + } + 'segmentation' { + markers = "${params.test_data_base}/data/imaging/segmentation/markers.csv" + image = "${params.test_data_base}/data/imaging/segmentation/cycif_tonsil_registered.ome.tif" + } + 'quantification' { + markers = "${params.test_data_base}/data/imaging/quantification/markers.csv" + image = "${params.test_data_base}/data/imaging/quantification/cycif_tonsil_registered.ome.tif" + mask = "${params.test_data_base}/data/imaging/quantification/cell.ome.tif" + } + 'downstream' { + markers = "${params.test_data_base}/data/imaging/downstream/markers.csv" + cell_feature_array = "${params.test_data_base}/data/imaging/downstream/cycif_tonsil_cell.csv" + } + 'background_subtraction' { + markers = "${params.test_data_base}/data/imaging/background_subtraction/markers.csv" + image = "${params.test_data_base}/data/imaging/background_subtraction/cycif_tonsil_registered.ome.tif" + } + 'core_detection' { + image = "${params.test_data_base}/data/imaging/core_detection/single_core_dapi.tif" + } + } + } +} diff --git a/lib/NftestUtils.groovy b/lib/NftestUtils.groovy new file mode 100644 index 0000000..ce6fc8b --- /dev/null +++ b/lib/NftestUtils.groovy @@ -0,0 +1,38 @@ +// Helper functions for pipeline tests + +class NftestUtils { + + // Function to remove Nextflow version from software_versions.yml + public static String removeNextflowVersion(outputDir) { + def softwareVersions = path("$outputDir/pipeline_info/software_versions.yml").yaml + if (softwareVersions.containsKey("Workflow")) { + softwareVersions.Workflow.remove("Nextflow") + } + return softwareVersions + } + + // Function to filter lines from a file and return a new file + public static File filterLines(String inFilePath, int linesToSkip) { + if (linesToSkip >= 0) { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lineCount = 0 + inputFile.eachLine { line -> + lineCount++ + if (lineCount > linesToSkip) { + outputFile.append(line + '\n') + } + } + return outputFile + } else { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lines = inputFile.readLines() + def totalLines = lines.size() + lines.take(totalLines + linesToSkip).each { line -> + outputFile.append(line + '\n') + } + return outputFile + } + } +} diff --git a/main.nf b/main.nf index 74c418b..0d8ca43 100644 --- a/main.nf +++ b/main.nf @@ -27,7 +27,7 @@ if (!params.fasta) { ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { validateParameters; paramsHelp } from 'plugin/nf-validation' +include { validateParameters; paramsHelp; fromSamplesheet } from 'plugin/nf-validation' // Print help message if needed if (params.help) { @@ -51,13 +51,17 @@ WorkflowMain.initialise(workflow, params, log) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { READSIMULATOR } from './workflows/readsimulator' +include { READSIMULATOR } from './workflows/readsimulator/main' // // WORKFLOW: Run main nf-core/readsimulator analysis pipeline // workflow NFCORE_READSIMULATOR { - READSIMULATOR () + + ch_input = Channel.fromSamplesheet("input") + ch_input.view() + + READSIMULATOR ( ch_input ) } /* diff --git a/nf-test.config b/nf-test.config new file mode 100644 index 0000000..2f9c518 --- /dev/null +++ b/nf-test.config @@ -0,0 +1,16 @@ +config { + // location for all nf-tests + testsDir "." + + // nf-test directory including temporary files for each test + workDir ".nf-tests" + + // location of library folder that is added automatically to the classpath + libDir "lib/" + + // location of an optional nextflow.config file specific for executing tests + configFile "nextflow.config" + + // run all test with the defined docker profile from the main nextflow.config + profile "" +} diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index f6612c1..291bec6 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -2,9 +2,9 @@ // Simulate amplicon reads // -include { CRABS_DBDOWNLOAD } from '../../modules/local/crabs/dbdownload/main' -include { CRABS_DBIMPORT } from '../../modules/local/crabs/dbimport/main' -include { CRABS_INSILICOPCR } from '../../modules/local/crabs/insilicopcr/main' +include { CRABS_DBDOWNLOAD } from '../../modules/local/crabs/dbdownload/main' // TODO: Add module to nf-core/modules +include { CRABS_DBIMPORT } from '../../modules/local/crabs/dbimport/main' // TODO: Add module to nf-core/modules +include { CRABS_INSILICOPCR } from '../../modules/local/crabs/insilicopcr/main' // TODO: Add module to nf-core/modules include { ART_ILLUMINA } from '../../modules/nf-core/art/illumina/main' workflow AMPLICON_WORKFLOW { diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index 8dbd736..c58a755 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -6,7 +6,7 @@ include { BOWTIE2_BUILD } from '../../modules/nf-core/bowtie2/ include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/main' include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' include { JAPSA_CAPSIM } from '../../modules/local/japsa/capsim/main' -include { UNZIP as UNZIP_PROBE } from '../../modules/local/unzip/main' +include { UNZIP as UNZIP_PROBE } from '../../modules/local/unzip/main' // TODO: Use nf-core/module here workflow TARGET_CAPTURE_WORKFLOW { take: diff --git a/workflows/readsimulator.nf b/workflows/readsimulator/main.nf similarity index 86% rename from workflows/readsimulator.nf rename to workflows/readsimulator/main.nf index b8ae3e4..929aaf7 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator/main.nf @@ -4,7 +4,7 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap; fromSamplesheet } from 'plugin/nf-validation' +include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) def citation = '\n' + WorkflowMain.citation(workflow) + '\n' @@ -35,16 +35,16 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // MODULE: Local modules // -include { INSILICOSEQ_GENERATE } from '../modules/local/insilicoseq/generate/main' -include { CREATE_SAMPLESHEET } from '../modules/local/custom/create_samplesheet/main' -include { MERGE_SAMPLESHEETS } from '../modules/local/custom/merge_samplesheets/main' -include { WGSIM } from '../modules/local/wgsim/main' +include { INSILICOSEQ_GENERATE } from '../../modules/local/insilicoseq/generate/main' // TODO: Add module to nf-core/modules +include { CREATE_SAMPLESHEET } from '../../modules/local/custom/create_samplesheet/main' +include { MERGE_SAMPLESHEETS } from '../../modules/local/custom/merge_samplesheets/main' +include { WGSIM } from '../../modules/local/wgsim/main' // TODO: Add module to nf-core/modules // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { AMPLICON_WORKFLOW } from '../subworkflows/local/amplicon_workflow' -include { TARGET_CAPTURE_WORKFLOW } from '../subworkflows/local/target_capture_workflow' +include { AMPLICON_WORKFLOW } from '../../subworkflows/local/amplicon_workflow' +include { TARGET_CAPTURE_WORKFLOW } from '../../subworkflows/local/target_capture_workflow' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -55,9 +55,9 @@ include { TARGET_CAPTURE_WORKFLOW } from '../subworkflows/local/target_capture_w // // MODULE: Installed directly from nf-core/modules // -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { FASTQC } from '../../modules/nf-core/fastqc/main' +include { MULTIQC } from '../../modules/nf-core/multiqc/main' +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../../modules/nf-core/custom/dumpsoftwareversions/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -70,8 +70,11 @@ def multiqc_report = [] workflow READSIMULATOR { + take: + ch_input + + main: ch_versions = Channel.empty() - ch_input = Channel.fromSamplesheet("input") ch_simulated_reads = Channel.empty() if ( params.fasta ) { @@ -158,7 +161,7 @@ workflow READSIMULATOR { meta, samplesheet -> tuple( meta.datatype, meta, samplesheet ) } - .groupTuple() + .groupTuple(sort: 'deep') .map { datatype, old_meta, samplesheet -> def meta = [:] @@ -167,7 +170,7 @@ workflow READSIMULATOR { } // MODULE: Merge the samplesheets by data type - MERGE_SAMPLESHEETS ( + ch_final_samplesheet = MERGE_SAMPLESHEETS ( ch_samplesheets ) @@ -205,6 +208,10 @@ workflow READSIMULATOR { ch_multiqc_logo.toList() ) multiqc_report = MULTIQC.out.report.toList() + + emit: + simulated_reads = ch_simulated_reads + samplesheet = ch_final_samplesheet } /* diff --git a/workflows/readsimulator/tests/tags.yml b/workflows/readsimulator/tests/tags.yml new file mode 100644 index 0000000..cd12567 --- /dev/null +++ b/workflows/readsimulator/tests/tags.yml @@ -0,0 +1,4 @@ +readsimulator_test_amplicon: + - ./** +readsimulator_test_wholegenome: + - ./** diff --git a/workflows/readsimulator/tests/test_amplicon.nf.test b/workflows/readsimulator/tests/test_amplicon.nf.test new file mode 100644 index 0000000..40f35a6 --- /dev/null +++ b/workflows/readsimulator/tests/test_amplicon.nf.test @@ -0,0 +1,40 @@ +nextflow_workflow { + + name "Test workflow: READSIMULATOR" + script "../main.nf" + workflow "READSIMULATOR" + tag "workflows" + tag "readsimulator" + tag "readsimulator_test_amplicon" + + test("amplicon = true") { + + when { + workflow { + """ + input[0] = Channel.of( + [ [ id:'first', seed:40] ], + [ [ id:'second', seed:41] ], + [ [ id:'third', seed:42] ], + ) + """ + } + params { + amplicon = true + fw_primer = 'AAAATAAT' + rv_primer = 'GATTACTTT' + amplicon_read_count = 1000 + fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/testdata/GCF_024334085.1_ASM2433408v1_genomic.fna.gz' + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot( + workflow.out + ).match() } + ) + } + } +} diff --git a/workflows/readsimulator/tests/test_amplicon.nf.test.snap b/workflows/readsimulator/tests/test_amplicon.nf.test.snap new file mode 100644 index 0000000..dd20dea --- /dev/null +++ b/workflows/readsimulator/tests/test_amplicon.nf.test.snap @@ -0,0 +1,101 @@ +{ + "amplicon = true": { + "content": [ + { + "0": [ + [ + { + "id": "first", + "seed": 40, + "outdir": "art_illumina", + "datatype": "amplicon_illumina" + }, + [ + "first1.fq.gz:md5,4070b67c9df68abbb0774bda0c4091e4", + "first2.fq.gz:md5,1f5564908b3fdbbdc9d89be640f4d7d9" + ] + ], + [ + { + "id": "second", + "seed": 41, + "outdir": "art_illumina", + "datatype": "amplicon_illumina" + }, + [ + "second1.fq.gz:md5,6a2d16db3e72c999cb8987a9bf1fea8a", + "second2.fq.gz:md5,1adcaee3f93687f36823ad83205cb5ac" + ] + ], + [ + { + "id": "third", + "seed": 42, + "outdir": "art_illumina", + "datatype": "amplicon_illumina" + }, + [ + "third1.fq.gz:md5,8af34387fa0b3486dfd4b360b52b04b6", + "third2.fq.gz:md5,f2755dacd8e22b8f13178058bad184d8" + ] + ] + ], + "1": [ + [ + { + "id": "amplicon_illumina" + }, + "amplicon_illumina_samplesheet.csv:md5,2f0440b621dd2de3c89cda85c81e5be7" + ] + ], + "samplesheet": [ + [ + { + "id": "amplicon_illumina" + }, + "amplicon_illumina_samplesheet.csv:md5,2f0440b621dd2de3c89cda85c81e5be7" + ] + ], + "simulated_reads": [ + [ + { + "id": "first", + "seed": 40, + "outdir": "art_illumina", + "datatype": "amplicon_illumina" + }, + [ + "first1.fq.gz:md5,4070b67c9df68abbb0774bda0c4091e4", + "first2.fq.gz:md5,1f5564908b3fdbbdc9d89be640f4d7d9" + ] + ], + [ + { + "id": "second", + "seed": 41, + "outdir": "art_illumina", + "datatype": "amplicon_illumina" + }, + [ + "second1.fq.gz:md5,6a2d16db3e72c999cb8987a9bf1fea8a", + "second2.fq.gz:md5,1adcaee3f93687f36823ad83205cb5ac" + ] + ], + [ + { + "id": "third", + "seed": 42, + "outdir": "art_illumina", + "datatype": "amplicon_illumina" + }, + [ + "third1.fq.gz:md5,8af34387fa0b3486dfd4b360b52b04b6", + "third2.fq.gz:md5,f2755dacd8e22b8f13178058bad184d8" + ] + ] + ] + } + ], + "timestamp": "2024-01-19T00:02:50.960492121" + } +} diff --git a/workflows/readsimulator/tests/test_metagenome.nf.test b/workflows/readsimulator/tests/test_metagenome.nf.test new file mode 100644 index 0000000..ee53e2d --- /dev/null +++ b/workflows/readsimulator/tests/test_metagenome.nf.test @@ -0,0 +1,38 @@ +nextflow_workflow { + + name "Test workflow: READSIMULATOR" + script "../main.nf" + workflow "READSIMULATOR" + tag "workflows" + tag "readsimulator" + tag "readsimulator_test_metagenome" + + test("metagenome = true") { + + when { + workflow { + """ + input[0] = Channel.of( + [ [ id:'first', seed:40] ], + [ [ id:'second', seed:41] ], + [ [ id:'third', seed:42] ], + ) + """ + } + params { + metagenome = true + metagenome_n_reads = '100K' + fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/testdata/GCF_024334085.1_ASM2433408v1_genomic.fna.gz' + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot( + workflow.out + ).match() } + ) + } + } +} diff --git a/workflows/readsimulator/tests/test_metagenome.nf.test.snap b/workflows/readsimulator/tests/test_metagenome.nf.test.snap new file mode 100644 index 0000000..a28510d --- /dev/null +++ b/workflows/readsimulator/tests/test_metagenome.nf.test.snap @@ -0,0 +1,101 @@ +{ + "metagenome = true": { + "content": [ + { + "0": [ + [ + { + "id": "first", + "seed": 40, + "outdir": "insilicoseq", + "datatype": "metagenomic_illumina" + }, + [ + "first_R1.fastq.gz:md5,2a324062e0afb4d3eab8134479f317d4", + "first_R2.fastq.gz:md5,9e0c86c0ff15091734fe7d52039f7727" + ] + ], + [ + { + "id": "second", + "seed": 41, + "outdir": "insilicoseq", + "datatype": "metagenomic_illumina" + }, + [ + "second_R1.fastq.gz:md5,3536190205e1a6ba3609fe6a1567e0c7", + "second_R2.fastq.gz:md5,a91ff7cf5ea2069d541fc7d081c82bfb" + ] + ], + [ + { + "id": "third", + "seed": 42, + "outdir": "insilicoseq", + "datatype": "metagenomic_illumina" + }, + [ + "third_R1.fastq.gz:md5,137d1f3ced4783a0a45f6d7aed6da743", + "third_R2.fastq.gz:md5,3a78f893f1c12af70d13fe3df59eaa84" + ] + ] + ], + "1": [ + [ + { + "id": "metagenomic_illumina" + }, + "metagenomic_illumina_samplesheet.csv:md5,8064936eff731e7c4c879916eb8b2edb" + ] + ], + "samplesheet": [ + [ + { + "id": "metagenomic_illumina" + }, + "metagenomic_illumina_samplesheet.csv:md5,8064936eff731e7c4c879916eb8b2edb" + ] + ], + "simulated_reads": [ + [ + { + "id": "first", + "seed": 40, + "outdir": "insilicoseq", + "datatype": "metagenomic_illumina" + }, + [ + "first_R1.fastq.gz:md5,2a324062e0afb4d3eab8134479f317d4", + "first_R2.fastq.gz:md5,9e0c86c0ff15091734fe7d52039f7727" + ] + ], + [ + { + "id": "second", + "seed": 41, + "outdir": "insilicoseq", + "datatype": "metagenomic_illumina" + }, + [ + "second_R1.fastq.gz:md5,3536190205e1a6ba3609fe6a1567e0c7", + "second_R2.fastq.gz:md5,a91ff7cf5ea2069d541fc7d081c82bfb" + ] + ], + [ + { + "id": "third", + "seed": 42, + "outdir": "insilicoseq", + "datatype": "metagenomic_illumina" + }, + [ + "third_R1.fastq.gz:md5,137d1f3ced4783a0a45f6d7aed6da743", + "third_R2.fastq.gz:md5,3a78f893f1c12af70d13fe3df59eaa84" + ] + ] + ] + } + ], + "timestamp": "2024-01-19T00:09:35.115953915" + } +} \ No newline at end of file diff --git a/workflows/readsimulator/tests/test_target_capture.nf.test b/workflows/readsimulator/tests/test_target_capture.nf.test new file mode 100644 index 0000000..ef73254 --- /dev/null +++ b/workflows/readsimulator/tests/test_target_capture.nf.test @@ -0,0 +1,38 @@ +nextflow_workflow { + + name "Test workflow: READSIMULATOR" + script "../main.nf" + workflow "READSIMULATOR" + tag "workflows" + tag "readsimulator" + tag "readsimulator_test_target_capture" + + test("target_capture = true") { + + when { + workflow { + """ + input[0] = Channel.of( + [ [ id:'first', seed:40] ], + [ [ id:'second', seed:41] ], + [ [ id:'third', seed:42] ], + ) + """ + } + params { + target_capture = true + probe_ref_name = 'Diptera-2.7Kv1' + fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/testdata/GCF_024334085.1_ASM2433408v1_genomic.fna.gz' + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot( + workflow.out + ).match() } + ) + } + } +} diff --git a/workflows/readsimulator/tests/test_target_capture.nf.test.snap b/workflows/readsimulator/tests/test_target_capture.nf.test.snap new file mode 100644 index 0000000..f34ebaf --- /dev/null +++ b/workflows/readsimulator/tests/test_target_capture.nf.test.snap @@ -0,0 +1,101 @@ +{ + "target_capture = true": { + "content": [ + { + "0": [ + [ + { + "id": "first", + "seed": 40, + "outdir": "capsim", + "datatype": "target_capture" + }, + [ + "first_1.fastq.gz:md5,a0d28da7897cdce5380fd803ca43fbd8", + "first_2.fastq.gz:md5,4b72a945f433797a05635996d7e41bdf" + ] + ], + [ + { + "id": "second", + "seed": 41, + "outdir": "capsim", + "datatype": "target_capture" + }, + [ + "second_1.fastq.gz:md5,3cb9456d6373288c877eb59ef01cb4b1", + "second_2.fastq.gz:md5,c5d09485a7b3dffba13fd5f8c827c498" + ] + ], + [ + { + "id": "third", + "seed": 42, + "outdir": "capsim", + "datatype": "target_capture" + }, + [ + "third_1.fastq.gz:md5,b7d11fb1d6fe768d17ee9940ed90d03c", + "third_2.fastq.gz:md5,995ea1c4eb2bcbb55b252dac290def09" + ] + ] + ], + "1": [ + [ + { + "id": "target_capture" + }, + "target_capture_samplesheet.csv:md5,5279e81ea6bcdee0e3d9e54f7cef1dab" + ] + ], + "samplesheet": [ + [ + { + "id": "target_capture" + }, + "target_capture_samplesheet.csv:md5,5279e81ea6bcdee0e3d9e54f7cef1dab" + ] + ], + "simulated_reads": [ + [ + { + "id": "first", + "seed": 40, + "outdir": "capsim", + "datatype": "target_capture" + }, + [ + "first_1.fastq.gz:md5,a0d28da7897cdce5380fd803ca43fbd8", + "first_2.fastq.gz:md5,4b72a945f433797a05635996d7e41bdf" + ] + ], + [ + { + "id": "second", + "seed": 41, + "outdir": "capsim", + "datatype": "target_capture" + }, + [ + "second_1.fastq.gz:md5,3cb9456d6373288c877eb59ef01cb4b1", + "second_2.fastq.gz:md5,c5d09485a7b3dffba13fd5f8c827c498" + ] + ], + [ + { + "id": "third", + "seed": 42, + "outdir": "capsim", + "datatype": "target_capture" + }, + [ + "third_1.fastq.gz:md5,b7d11fb1d6fe768d17ee9940ed90d03c", + "third_2.fastq.gz:md5,995ea1c4eb2bcbb55b252dac290def09" + ] + ] + ] + } + ], + "timestamp": "2024-01-19T00:15:17.918173096" + } +} \ No newline at end of file diff --git a/workflows/readsimulator/tests/test_wholegenome.nf.test b/workflows/readsimulator/tests/test_wholegenome.nf.test new file mode 100644 index 0000000..1ad3498 --- /dev/null +++ b/workflows/readsimulator/tests/test_wholegenome.nf.test @@ -0,0 +1,37 @@ +nextflow_workflow { + + name "Test workflow: READSIMULATOR" + script "../main.nf" + workflow "READSIMULATOR" + tag "workflows" + tag "readsimulator" + tag "readsimulator_test_wholegenome" + + test("wholegenome = true") { + + when { + workflow { + """ + input[0] = Channel.of( + [ [ id:'first', seed:40] ], + [ [ id:'second', seed:41] ], + [ [ id:'third', seed:42] ], + ) + """ + } + params { + wholegenome = true + fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/testdata/GCF_024334085.1_ASM2433408v1_genomic.fna.gz' + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot( + workflow.out + ).match() } + ) + } + } +} diff --git a/workflows/readsimulator/tests/test_wholegenome.nf.test.snap b/workflows/readsimulator/tests/test_wholegenome.nf.test.snap new file mode 100644 index 0000000..0d4e785 --- /dev/null +++ b/workflows/readsimulator/tests/test_wholegenome.nf.test.snap @@ -0,0 +1,101 @@ +{ + "wholegenome = true": { + "content": [ + { + "0": [ + [ + { + "id": "first", + "seed": 40, + "outdir": "wgsim", + "datatype": "wholegenome" + }, + [ + "first_R1.fq.gz:md5,90ebdcc4f7783290afe3e6ee02d6d803", + "first_R2.fq.gz:md5,bfb972c268a36edcef29317fd1ced544" + ] + ], + [ + { + "id": "second", + "seed": 41, + "outdir": "wgsim", + "datatype": "wholegenome" + }, + [ + "second_R1.fq.gz:md5,7902aa5c9041fc9e2585ebdd0c83597e", + "second_R2.fq.gz:md5,fa9f52716714e16d354e575c331d4ffc" + ] + ], + [ + { + "id": "third", + "seed": 42, + "outdir": "wgsim", + "datatype": "wholegenome" + }, + [ + "third_R1.fq.gz:md5,a76e6d7ab7a4f4bae18747ae6abb607e", + "third_R2.fq.gz:md5,e42714cc210d161fbb3df55c75e96d61" + ] + ] + ], + "1": [ + [ + { + "id": "wholegenome" + }, + "wholegenome_samplesheet.csv:md5,310b2ee2f03a2b44beb88da1816bc3d0" + ] + ], + "samplesheet": [ + [ + { + "id": "wholegenome" + }, + "wholegenome_samplesheet.csv:md5,310b2ee2f03a2b44beb88da1816bc3d0" + ] + ], + "simulated_reads": [ + [ + { + "id": "first", + "seed": 40, + "outdir": "wgsim", + "datatype": "wholegenome" + }, + [ + "first_R1.fq.gz:md5,90ebdcc4f7783290afe3e6ee02d6d803", + "first_R2.fq.gz:md5,bfb972c268a36edcef29317fd1ced544" + ] + ], + [ + { + "id": "second", + "seed": 41, + "outdir": "wgsim", + "datatype": "wholegenome" + }, + [ + "second_R1.fq.gz:md5,7902aa5c9041fc9e2585ebdd0c83597e", + "second_R2.fq.gz:md5,fa9f52716714e16d354e575c331d4ffc" + ] + ], + [ + { + "id": "third", + "seed": 42, + "outdir": "wgsim", + "datatype": "wholegenome" + }, + [ + "third_R1.fq.gz:md5,a76e6d7ab7a4f4bae18747ae6abb607e", + "third_R2.fq.gz:md5,e42714cc210d161fbb3df55c75e96d61" + ] + ] + ] + } + ], + "timestamp": "2024-01-19T00:04:12.073328968" + } +} From 11bbb3b2f724ca4ab7840f16693ce7d55c1a267b Mon Sep 17 00:00:00 2001 From: Carson J Miller Date: Fri, 19 Jan 2024 16:18:07 +0000 Subject: [PATCH 68/89] Fixed CI --- .github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index ec2de1f..947c874 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -104,7 +104,7 @@ jobs: - name: Run nf-test run: | - nf-test test --verbose --tag ${{ matrix.tags }} --profile test,test_data,"${{ matrix.profile }}" --junitxml=test.xml --tap=test.tap + nf-test test --verbose --tag ${{ matrix.tags }} --profile test,"${{ matrix.profile }}" --junitxml=test.xml --tap=test.tap - uses: pcolby/tap-summary@v1 with: From c1148867fb03f031d4287e7b5870ed76a089ff0d Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Fri, 19 Jan 2024 18:58:09 +0000 Subject: [PATCH 69/89] [automated] Fix linting with Prettier --- .devcontainer/devcontainer.json | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe..4a9bc5c 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.linting.pylintPath": "/opt/conda/bin/pylint", }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] - } - } + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], + }, + }, } From 27f8206e5c1ddc9aa032d09ebe7863016311f86d Mon Sep 17 00:00:00 2001 From: Carson J Miller Date: Fri, 19 Jan 2024 18:59:01 +0000 Subject: [PATCH 70/89] set actions_ci to false --- .nf-core.yml | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/.nf-core.yml b/.nf-core.yml index 17ef22b..005c4f3 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,2 +1,3 @@ repository_type: pipeline -actions_ci: false +lint: + actions_ci: false From 9a619db56e170ad2a676d349eeb287a2300c5602 Mon Sep 17 00:00:00 2001 From: Carson J Miller Date: Fri, 19 Jan 2024 19:23:26 +0000 Subject: [PATCH 71/89] Added option to make amplicon output reproducible --- conf/modules.config | 2 +- nextflow.config | 1 + nextflow_schema.json | 16 +++++------ .../readsimulator/tests/test_amplicon.nf.test | 11 ++++---- .../tests/test_amplicon.nf.test.snap | 28 +++++++++---------- 5 files changed, 30 insertions(+), 28 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 2b71267..6d45e43 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -71,7 +71,7 @@ process { } withName: CRABS_INSILICOPCR { - ext.args = "--error 4.5 -t 12 --fwd ${params.amplicon_fw_primer} --rev ${params.amplicon_rv_primer}" + ext.args = "--error ${params.amplicon_crabs_ispcr_error} -t 12 --fwd ${params.amplicon_fw_primer} --rev ${params.amplicon_rv_primer}" publishDir = [ path: { "${params.outdir}/crabs_insilicopcr" }, mode: params.publish_dir_mode diff --git a/nextflow.config b/nextflow.config index 3677248..869868a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -33,6 +33,7 @@ params { amplicon_read_count = 500 amplicon_read_length = 130 amplicon_seq_system = 'HS25' + amplicon_crabs_ispcr_error = 4.5 // Target capture options probe_file = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 408f552..2eb5a22 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -52,25 +52,21 @@ "amplicon": { "type": "boolean", "description": "Option to simulate amplicon sequencing reads.", - "default": false, "fa_icon": "fas fa-question-circle" }, "target_capture": { "type": "boolean", "description": "Option to simulate target capture sequencing reads.", - "default": false, "fa_icon": "fas fa-question-circle" }, "metagenome": { "type": "boolean", "description": "Option to simulate metagenomic sequencing reads.", - "default": false, "fa_icon": "fas fa-question-circle" }, "wholegenome": { "type": "boolean", "description": "Option to simulate wholegenomic sequencing reads.", - "default": false, "fa_icon": "fas fa-question-circle" } } @@ -107,6 +103,11 @@ "default": "HS25", "help_text": "Can be 'GA1' for Genome Analyser I, 'GA2' for Genome Analyser II, 'HS10' for HiSeq 1000, 'HS20' for HiSeq 2000, 'HS25' for HiSeq 2500, 'HSXn' for HiSeqX PCR free, 'HSXt' for HiSeqX TruSeq, 'MinS' for MiniSeq TruSeq, 'MSv1' for MiSeq v1, 'MSv3' for MiSeq v3, or 'NS50' for NextSeq500 v2.", "enum": ["GA1", "GA2", "HS10", "HS20", "HS25", "HSXn", "HSXt", "MinS", "MSv1", "MSv3", "NS50"] + }, + "amplicon_crabs_ispcr_error": { + "type": "number", + "default": 4.5, + "description": "Maximum number of errors allowed in CRABS insilicoPCR primer sequences" } } }, @@ -157,7 +158,7 @@ }, "target_capture_fshape": { "type": "number", - "default": 6.0, + "default": 6, "description": "Shape parameter of the fragment size distribution." }, "target_capture_smedian": { @@ -167,7 +168,7 @@ }, "target_capture_sshape": { "type": "number", - "default": 6.0, + "default": 6, "description": "Shape parameter of the fragment size distribution." }, "target_capture_tmedian": { @@ -358,8 +359,7 @@ "format": "file-path", "exists": true, "mimetype": "text/plain", - "description": "Path to text file containing taxids (one taxid per row).", - "help_text": "" + "description": "Path to text file containing taxids (one taxid per row)." }, "ncbidownload_group": { "type": "string", diff --git a/workflows/readsimulator/tests/test_amplicon.nf.test b/workflows/readsimulator/tests/test_amplicon.nf.test index 40f35a6..3eccfd0 100644 --- a/workflows/readsimulator/tests/test_amplicon.nf.test +++ b/workflows/readsimulator/tests/test_amplicon.nf.test @@ -20,11 +20,12 @@ nextflow_workflow { """ } params { - amplicon = true - fw_primer = 'AAAATAAT' - rv_primer = 'GATTACTTT' - amplicon_read_count = 1000 - fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/testdata/GCF_024334085.1_ASM2433408v1_genomic.fna.gz' + amplicon = true + fw_primer = 'AAAATAAT' + rv_primer = 'GATTACTTT' + amplicon_read_count = 1000 + amplicon_crabs_ispcr_error = 0 + fasta = 'https://raw.githubusercontent.com/nf-core/test-datasets/readsimulator/testdata/GCF_024334085.1_ASM2433408v1_genomic.fna.gz' } } diff --git a/workflows/readsimulator/tests/test_amplicon.nf.test.snap b/workflows/readsimulator/tests/test_amplicon.nf.test.snap index dd20dea..09f0d95 100644 --- a/workflows/readsimulator/tests/test_amplicon.nf.test.snap +++ b/workflows/readsimulator/tests/test_amplicon.nf.test.snap @@ -11,8 +11,8 @@ "datatype": "amplicon_illumina" }, [ - "first1.fq.gz:md5,4070b67c9df68abbb0774bda0c4091e4", - "first2.fq.gz:md5,1f5564908b3fdbbdc9d89be640f4d7d9" + "first1.fq.gz:md5,d41d8cd98f00b204e9800998ecf8427e", + "first2.fq.gz:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], [ @@ -23,8 +23,8 @@ "datatype": "amplicon_illumina" }, [ - "second1.fq.gz:md5,6a2d16db3e72c999cb8987a9bf1fea8a", - "second2.fq.gz:md5,1adcaee3f93687f36823ad83205cb5ac" + "second1.fq.gz:md5,d41d8cd98f00b204e9800998ecf8427e", + "second2.fq.gz:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], [ @@ -35,8 +35,8 @@ "datatype": "amplicon_illumina" }, [ - "third1.fq.gz:md5,8af34387fa0b3486dfd4b360b52b04b6", - "third2.fq.gz:md5,f2755dacd8e22b8f13178058bad184d8" + "third1.fq.gz:md5,d41d8cd98f00b204e9800998ecf8427e", + "third2.fq.gz:md5,d41d8cd98f00b204e9800998ecf8427e" ] ] ], @@ -65,8 +65,8 @@ "datatype": "amplicon_illumina" }, [ - "first1.fq.gz:md5,4070b67c9df68abbb0774bda0c4091e4", - "first2.fq.gz:md5,1f5564908b3fdbbdc9d89be640f4d7d9" + "first1.fq.gz:md5,d41d8cd98f00b204e9800998ecf8427e", + "first2.fq.gz:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], [ @@ -77,8 +77,8 @@ "datatype": "amplicon_illumina" }, [ - "second1.fq.gz:md5,6a2d16db3e72c999cb8987a9bf1fea8a", - "second2.fq.gz:md5,1adcaee3f93687f36823ad83205cb5ac" + "second1.fq.gz:md5,d41d8cd98f00b204e9800998ecf8427e", + "second2.fq.gz:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], [ @@ -89,13 +89,13 @@ "datatype": "amplicon_illumina" }, [ - "third1.fq.gz:md5,8af34387fa0b3486dfd4b360b52b04b6", - "third2.fq.gz:md5,f2755dacd8e22b8f13178058bad184d8" + "third1.fq.gz:md5,d41d8cd98f00b204e9800998ecf8427e", + "third2.fq.gz:md5,d41d8cd98f00b204e9800998ecf8427e" ] ] ] } ], - "timestamp": "2024-01-19T00:02:50.960492121" + "timestamp": "2024-01-19T19:15:08.881730553" } -} +} \ No newline at end of file From c86addc88fffa4a6913b56d00b2e1897755575ab Mon Sep 17 00:00:00 2001 From: Carson J Miller Date: Mon, 22 Jan 2024 16:25:19 +0000 Subject: [PATCH 72/89] Addressed PR comments --- main.nf | 1 - workflows/readsimulator/main.nf | 36 ++++++++++++++++++++++++++++++--- 2 files changed, 33 insertions(+), 4 deletions(-) diff --git a/main.nf b/main.nf index 0d8ca43..b11a216 100644 --- a/main.nf +++ b/main.nf @@ -59,7 +59,6 @@ include { READSIMULATOR } from './workflows/readsimulator/main' workflow NFCORE_READSIMULATOR { ch_input = Channel.fromSamplesheet("input") - ch_input.view() READSIMULATOR ( ch_input ) } diff --git a/workflows/readsimulator/main.nf b/workflows/readsimulator/main.nf index 929aaf7..c4ba8c7 100644 --- a/workflows/readsimulator/main.nf +++ b/workflows/readsimulator/main.nf @@ -56,6 +56,7 @@ include { TARGET_CAPTURE_WORKFLOW } from '../../subworkflows/local/target_captur // MODULE: Installed directly from nf-core/modules // include { FASTQC } from '../../modules/nf-core/fastqc/main' +include { NCBIGENOMEDOWNLOAD } from '../../modules/nf-core/ncbigenomedownload/main' include { MULTIQC } from '../../modules/nf-core/multiqc/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../../modules/nf-core/custom/dumpsoftwareversions/main' @@ -76,15 +77,44 @@ workflow READSIMULATOR { main: ch_versions = Channel.empty() ch_simulated_reads = Channel.empty() + ch_taxids = Channel.empty() + ch_accessions = Channel.empty() if ( params.fasta ) { ch_fasta = Channel.fromPath(params.fasta) } else { - ch_fasta = Channel.empty() + if ( params.ncbidownload_accessions ) { + ch_accessions = Channel.fromPath(params.ncbidownload_accessions) + } else if ( params.ncbidownload_taxids ) { + ch_taxids = Channel.fromPath(params.ncbidownload_taxids) + } + + // + // MODULE: Download reference fasta files + // + NCBIGENOMEDOWNLOAD ( + [ id:"ncbigenomedownload" ], + ch_accessions.ifEmpty([]), + ch_taxids.ifEmpty([]), + params.ncbidownload_group + ) + + // + // MODULE: Combine FASTA files + // + MERGE_FASTAS ( + NCBIGENOMEDOWNLOAD.out.fna + ) + + ch_fasta = MERGE_FASTAS.out.fasta + .map { + meta, fasta -> + return fasta + } } - if ( params.probe_fasta ) { - ch_probes = Channel.fromPath(params.probe_fasta) + if ( params.probe_file ) { + ch_probes = Channel.fromPath(params.probe_file) } else { ch_probes = Channel.empty() } From ae2352681df7e593c31292887300a5fc7111c415 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 29 Jan 2024 15:13:14 +0000 Subject: [PATCH 73/89] Template update for nf-core/tools version 2.12 --- .editorconfig | 8 + .github/workflows/awsfulltest.yml | 2 +- .github/workflows/awstest.yml | 2 +- .github/workflows/branch.yml | 2 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 67 +++++ .github/workflows/fix-linting.yml | 72 +++-- .github/workflows/linting.yml | 61 +--- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 4 +- .gitpod.yml | 1 + .pre-commit-config.yaml | 7 +- README.md | 8 +- assets/email_template.html | 2 +- assets/email_template.txt | 2 +- assets/nf-core-readsimulator_logo_light.png | Bin 84886 -> 98327 bytes .../nf-core-readsimulator_logo_dark.png | Bin 84983 -> 26177 bytes .../nf-core-readsimulator_logo_light.png | Bin 84886 -> 22145 bytes lib/WorkflowMain.groovy | 16 +- lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes main.nf | 2 +- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- .../dumpsoftwareversions/tests/main.nf.test | 7 +- .../tests/main.nf.test.snap | 50 ++-- modules/nf-core/fastqc/tests/main.nf.test | 265 ++++++++++++------ .../nf-core/fastqc/tests/main.nf.test.snap | 12 +- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/multiqc/meta.yml | 1 - modules/nf-core/multiqc/tests/main.nf.test | 48 +++- .../nf-core/multiqc/tests/main.nf.test.snap | 21 ++ nextflow.config | 3 +- pyproject.toml | 17 +- workflows/readsimulator.nf | 7 + 36 files changed, 490 insertions(+), 221 deletions(-) create mode 100644 .github/workflows/download_pipeline.yml delete mode 100644 lib/nfcore_external_java_deps.jar create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap diff --git a/.editorconfig b/.editorconfig index b6b3190..9b99008 100644 --- a/.editorconfig +++ b/.editorconfig @@ -22,3 +22,11 @@ indent_size = unset [/assets/email*] indent_size = unset + +# ignore Readme +[README.md] +indent_style = unset + +# ignore python +[*.{py}] +indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 9555e9f..bd01c3d 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 1e0d22e..ed0d641 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 8454e5b..c2775b1 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v1 + uses: mshick/add-pr-comment@v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index 694e90e..e37cfda 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v7 + - uses: actions/stale@v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml new file mode 100644 index 0000000..8611458 --- /dev/null +++ b/.github/workflows/download_pipeline.yml @@ -0,0 +1,67 @@ +name: Test successful pipeline download with 'nf-core download' + +# Run the workflow when: +# - dispatched manually +# - when a PR is opened or reopened to master branch +# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. +on: + workflow_dispatch: + pull_request: + types: + - opened + branches: + - master + pull_request_target: + branches: + - master + +env: + NXF_ANSI_LOG: false + +jobs: + download: + runs-on: ubuntu-latest + steps: + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + + - uses: actions/setup-python@v5 + with: + python-version: "3.11" + architecture: "x64" + - uses: eWaterCycle/setup-singularity@v7 + with: + singularity-version: 3.8.3 + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install git+https://github.com/nf-core/tools.git@dev + + - name: Get the repository name and current branch set as environment variable + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + + - name: Download the pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + run: | + nf-core download ${{ env.REPO_LOWERCASE }} \ + --revision ${{ env.REPO_BRANCH }} \ + --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + --compress "none" \ + --container-system 'singularity' \ + --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-cache-utilisation 'amend' \ + --download-configuration + + - name: Inspect download + run: tree ./${{ env.REPOTITLE_LOWERCASE }} + + - name: Run the downloaded pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 4d6d495..f5b18fa 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -4,7 +4,7 @@ on: types: [created] jobs: - deploy: + fix-linting: # Only run if comment is on a PR with the main repo, and if it contains the magic keywords if: > contains(github.event.comment.html_url, '/pull/') && @@ -13,10 +13,17 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} + # indication that the linting is being fixed + - name: React on comment + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: eyes + # Action runs on the issue comment, so we don't get the PR by default # Use the gh cli to check out the PR - name: Checkout Pull Request @@ -24,32 +31,59 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v4 + # Install and run pre-commit + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + with: + python-version: 3.11 - - name: Install Prettier - run: npm install -g prettier @prettier/plugin-php + - name: Install pre-commit + run: pip install pre-commit - # Check that we actually need to fix something - - name: Run 'prettier --check' - id: prettier_status - run: | - if prettier --check ${GITHUB_WORKSPACE}; then - echo "result=pass" >> $GITHUB_OUTPUT - else - echo "result=fail" >> $GITHUB_OUTPUT - fi + - name: Run pre-commit + id: pre-commit + run: pre-commit run --all-files + continue-on-error: true - - name: Run 'prettier --write' - if: steps.prettier_status.outputs.result == 'fail' - run: prettier --write ${GITHUB_WORKSPACE} + # indication that the linting has finished + - name: react if linting finished succesfully + if: steps.pre-commit.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: "+1" - name: Commit & push changes - if: steps.prettier_status.outputs.result == 'fail' + id: commit-and-push + if: steps.pre-commit.outcome == 'failure' run: | git config user.email "core@nf-co.re" git config user.name "nf-core-bot" git config push.default upstream git add . git status - git commit -m "[automated] Fix linting with Prettier" + git commit -m "[automated] Fix code linting" git push + + - name: react if linting errors were fixed + id: react-if-fixed + if: steps.commit-and-push.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: hooray + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: confused + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + issue-number: ${{ github.event.issue.number }} + body: | + @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually. + See [CI log](https://github.com/nf-core/readsimulator/actions/runs/${{ github.run_id }}) for more details. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 905c58e..81cd098 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,61 +11,22 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - uses: actions/checkout@v4 - - uses: actions/setup-node@v4 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - uses: actions/setup-node@v4 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest @@ -76,7 +37,7 @@ jobs: - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.11" architecture: "x64" @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bbcd30..147bcd1 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v2 + uses: dawidd6/action-download-artifact@v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 6ad3392..21ac3f0 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -24,7 +24,7 @@ jobs: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +56,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.0.2 + - uses: zentered/bluesky-post-action@v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/.gitpod.yml b/.gitpod.yml index acf7269..363d5b1 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -7,6 +7,7 @@ tasks: - name: unset JAVA_TOOL_OPTIONS command: | unset JAVA_TOOL_OPTIONS + vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 0c31cdb..af57081 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -1,5 +1,10 @@ repos: - repo: https://github.com/pre-commit/mirrors-prettier - rev: "v2.7.1" + rev: "v3.1.0" hooks: - id: prettier + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python + rev: "2.7.3" + hooks: + - id: editorconfig-checker + alias: ec diff --git a/README.md b/README.md index 77990b6..78caf82 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,9 @@ -# ![nf-core/readsimulator](docs/images/nf-core-readsimulator_logo_light.png#gh-light-mode-only) ![nf-core/readsimulator](docs/images/nf-core-readsimulator_logo_dark.png#gh-dark-mode-only) - +

    + + + nf-core/readsimulator + +

    [![GitHub Actions CI Status](https://github.com/nf-core/readsimulator/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/readsimulator/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/readsimulator/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/readsimulator/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/readsimulator/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) diff --git a/assets/email_template.html b/assets/email_template.html index 1137716..f2bc049 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -12,7 +12,7 @@ -

    nf-core/readsimulator v${version}

    +

    nf-core/readsimulator ${version}

    Run Name: $runName

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Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } } diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar deleted file mode 100644 index 805c8bb5e4fd43a12a5891eea5a68788309629b0..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX zni_mKLGakt&P12{r^KCD0GPa9sIuy`fu+agt?8O-hY9i{WloGe}y@kSsDEY_Fud9 z-?2ab4J)MQXe4E3Xl-U<>PRMQZ)0m@@90KnV5#Te5Tz`pvZ0Lj4F<9%Flh(`Qb;Tn zMV!iFmH{S^fF*j#MCKRUd`7m>9||*;WE32I_6pg4-Q1C#ke$$aVmP-TtfJ+j+N9;Q z>bxN=bCVQkg$Jzty71(+~A9}ZfANuYIVnTdW!??Sj)Ti-Qpk8XgPDab8pk2QD&dwUl=iFhd1c$C2uMVagr zWc@z9s$#g__{3?VGb#)^fSNp^lZh zU>MI!D?+L)nyth{@@L0+B&_M8iR08L)G6#PZQLFmvz-9DlZkwRWigRIyI4xYr7>tRJ-N6MlBB`nJQ!!8D)mI2lB3PP;dC{<{Td>^ 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b/modules.json index 7ed6f2c..39ebfe9 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", + "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "4ab13872435962dadc239979554d13709e20bf29", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index f0c63f6..9b3272b 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 7685b33..f218761 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index eec1db1..b1e1630 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,7 +31,12 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } ) } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 4274ed5..5f59a93 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,27 +1,33 @@ { "Should run without failures": { "content": [ - { - "0": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ], - "1": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "2": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "mqc_yml": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "versions": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "yml": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ] - } + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] ], - "timestamp": "2023-11-03T14:43:22.157011" + "timestamp": "2024-01-09T23:01:18.710682" } -} +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index b9e8f92..1f21c66 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -3,24 +3,20 @@ nextflow_process { name "Test Process FASTQC" script "../main.nf" process "FASTQC" + tag "modules" tag "modules_nfcore" tag "fastqc" - test("Single-Read") { + test("sarscov2 single-end [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ - input[0] = [ + input[0] = Channel.of([ [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) """ } } @@ -28,82 +24,189 @@ nextflow_process { then { assertAll ( { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. // looks like this:
    Mon 2 Oct 2023
    test.gz
    // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, - { assert path(process.out.html.get(0).get(1)).getText().contains("") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } ) } } -// TODO -// // -// // Test with paired-end data -// // -// workflow test_fastqc_paired_end { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with interleaved data -// // -// workflow test_fastqc_interleaved { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with bam data -// // -// workflow test_fastqc_bam { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with multiple samples -// // -// workflow test_fastqc_multiple { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with custom prefix -// // -// workflow test_fastqc_custom_prefix { -// input = [ -// [ id:'mysample', single_end:true ], // meta map -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + { assert path(process.out.html[0][1][2]).text.contains("") }, + { assert path(process.out.html[0][1][3]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match() } + ) + } + } + } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 636a32c..5d624bb 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,10 +1,20 @@ { + "sarscov2 single-end [fastq] - stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2024-01-17T18:40:57.254299" + }, "versions": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2023-10-09T23:40:54+0000" + "timestamp": "2024-01-17T18:36:50.033627" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index bc0bdb5..7625b75 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.18 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 00cc48d..1b9f7c4 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : - 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -43,7 +43,7 @@ process MULTIQC { stub: """ - touch multiqc_data + mkdir multiqc_data touch multiqc_plots touch multiqc_report.html diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f1aa660..45a9bc3 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,4 +1,3 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index c2dad21..d0438ed 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -7,12 +7,9 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" - test("MULTIQC: FASTQC") { + test("sarscov2 single-end [fastqc]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -26,20 +23,17 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } ) } } - test("MULTIQC: FASTQC and a config file") { + test("sarscov2 single-end [fastqc] [config]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -53,9 +47,35 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastqc] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.report.collect { file(it).getName() } + + process.out.data.collect { file(it).getName() } + + process.out.plots.collect { file(it).getName() } + + process.out.versions ).match() } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap new file mode 100644 index 0000000..d37e730 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -0,0 +1,21 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:02:49.911994" + }, + "sarscov2 single-end [fastqc] - stub": { + "content": [ + [ + "multiqc_report.html", + "multiqc_data", + "multiqc_plots", + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:03:14.524346" + } +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index a6a8494..47b6d8f 100644 --- a/nextflow.config +++ b/nextflow.config @@ -71,7 +71,7 @@ try { } // Load nf-core/readsimulator custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! +// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! // try { // includeConfig "${params.custom_config_base}/pipeline/readsimulator.config" // } catch (Exception e) { @@ -91,6 +91,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false + channels = ['conda-forge', 'bioconda', 'defaults'] apptainer.enabled = false } mamba { diff --git a/pyproject.toml b/pyproject.toml index 0d62beb..7d08e1c 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,10 +1,13 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] +target-version = "py38" +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] +cache-dir = "~/.cache/ruff" -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 +[tool.ruff.isort] +known-first-party = ["nf_core"] + +[tool.ruff.per-file-ignores] +"__init__.py" = ["E402", "F401"] diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 44c3497..fc09a0a 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -127,6 +127,13 @@ workflow.onComplete { } } +workflow.onError { + if (workflow.errorReport.contains("Process requirement exceeds available memory")) { + println("🛑 Default resources exceed availability 🛑 ") + println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") + } +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END From f22cabd0a3e4a64248a02d42e9bbf9abe2eb251b Mon Sep 17 00:00:00 2001 From: "Carson J. Miller" Date: Tue, 30 Jan 2024 17:36:24 +0000 Subject: [PATCH 74/89] Updated nf-core modules --- modules.json | 4 +- .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- .../dumpsoftwareversions/tests/main.nf.test | 7 ++- .../tests/main.nf.test.snap | 50 +++++++++++-------- .../nf-core/samtools/index/environment.yml | 2 +- modules/nf-core/samtools/index/main.nf | 4 +- 7 files changed, 42 insertions(+), 31 deletions(-) diff --git a/modules.json b/modules.json index 1684926..9617eaf 100644 --- a/modules.json +++ b/modules.json @@ -28,7 +28,7 @@ }, "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] }, "fastqc": { @@ -48,7 +48,7 @@ }, "samtools/index": { "branch": "master", - "git_sha": "5394565c5fe4c760e5b35977ec7607c62e81d1f8", + "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index f0c63f6..9b3272b 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 7685b33..f218761 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index eec1db1..b1e1630 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,7 +31,12 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } ) } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 4274ed5..5f59a93 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,27 +1,33 @@ { "Should run without failures": { "content": [ - { - "0": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ], - "1": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "2": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "mqc_yml": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "versions": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "yml": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ] - } + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n
    Process Name \\", + " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    \\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] ], - "timestamp": "2023-11-03T14:43:22.157011" + "timestamp": "2024-01-09T23:01:18.710682" } -} +} \ No newline at end of file diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml index 3c6f95b..296ed99 100644 --- a/modules/nf-core/samtools/index/environment.yml +++ b/modules/nf-core/samtools/index/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.17 + - bioconda::samtools=1.18 diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf index 256bd7c..8ad18fd 100644 --- a/modules/nf-core/samtools/index/main.nf +++ b/modules/nf-core/samtools/index/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_INDEX { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(input) From 02bd34d31a14cb1c2de96562cf934192e22967e6 Mon Sep 17 00:00:00 2001 From: "Carson J. Miller" Date: Tue, 30 Jan 2024 17:55:32 +0000 Subject: [PATCH 75/89] Fixed loading of test_data.config --- conf/test.config | 2 -- nextflow.config | 2 +- 2 files changed, 1 insertion(+), 3 deletions(-) diff --git a/conf/test.config b/conf/test.config index 3a71f83..e472172 100644 --- a/conf/test.config +++ b/conf/test.config @@ -19,5 +19,3 @@ params { max_memory = '6.GB' max_time = '6.h' } - -includeConfig './test_data.config' diff --git a/nextflow.config b/nextflow.config index 869868a..2487eed 100644 --- a/nextflow.config +++ b/nextflow.config @@ -113,7 +113,7 @@ params { } // Load base.config by default for all pipelines -includeConfig 'conf/base.config' +includeConfig 'conf/test_data.config' // Load ref_databases.config for reference taxonomy includeConfig 'conf/ref_databases.config' From 5704b602ab2c8eb7c5657b5d6a08a21e975cbddc Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 31 Jan 2024 00:22:01 +0000 Subject: [PATCH 76/89] fixed params --- .devcontainer/devcontainer.json | 8 ++++---- .github/workflows/download_pipeline.yml | 2 +- nextflow.config | 6 +++--- 3 files changed, 8 insertions(+), 8 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4a9bc5c..4ecfbfe 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint", + "python.linting.pylintPath": "/opt/conda/bin/pylint" }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], - }, - }, + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] + } + } } diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458..8a33004 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -64,4 +64,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/nextflow.config b/nextflow.config index 9337154..30b2d03 100644 --- a/nextflow.config +++ b/nextflow.config @@ -61,15 +61,15 @@ params { metagenome_gc_bias = null // Whole Genome options - wholegenome_error_rate = 0.020 + wholegenome_error_rate = 0.02 wholegenome_outer_dist = 500 wholegenome_standard_dev = 50 wholegenome_n_reads = 1000000 wholegenome_r1_length = 70 wholegenome_r2_length = 70 - wholegenome_mutation_rate = 0.0010 + wholegenome_mutation_rate = 0.001 wholegenome_indel_fraction = 0.15 - wholegenome_indel_extended = 0.30 + wholegenome_indel_extended = 0.3 // MultiQC options multiqc_config = null From 7ff2bcc0a11116e78d4981f285dc2bcf7585829f Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 31 Jan 2024 01:04:01 +0000 Subject: [PATCH 77/89] Trying to fix bug intoduced by tool update --- modules/nf-core/fastqc/tests/main.nf.test | 21 +++++++++++++++++++++ modules/nf-core/multiqc/tests/main.nf.test | 9 +++++++++ 2 files changed, 30 insertions(+) diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 1f21c66..58fc73d 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -11,6 +11,9 @@ nextflow_process { test("sarscov2 single-end [fastq]") { when { + params { + outdir = "$outputDir" + } process { """ input[0] = Channel.of([ @@ -41,6 +44,9 @@ nextflow_process { test("sarscov2 paired-end [fastq]") { when { + params { + outdir = "$outputDir" + } process { """ input[0] = Channel.of([ @@ -71,6 +77,9 @@ nextflow_process { test("sarscov2 interleaved [fastq]") { when { + params { + outdir = "$outputDir" + } process { """ input[0] = Channel.of([ @@ -97,6 +106,9 @@ nextflow_process { test("sarscov2 paired-end [bam]") { when { + params { + outdir = "$outputDir" + } process { """ input[0] = Channel.of([ @@ -123,6 +135,9 @@ nextflow_process { test("sarscov2 multiple [fastq]") { when { + params { + outdir = "$outputDir" + } process { """ input[0] = Channel.of([ @@ -161,6 +176,9 @@ nextflow_process { test("sarscov2 custom_prefix") { when { + params { + outdir = "$outputDir" + } process { """ input[0] = Channel.of([ @@ -189,6 +207,9 @@ nextflow_process { options "-stub" when { + params { + outdir = "$outputDir" + } process { """ input[0] = Channel.of([ diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index d0438ed..9a0207b 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -10,6 +10,9 @@ nextflow_process { test("sarscov2 single-end [fastqc]") { when { + params { + outdir = "$outputDir" + } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -34,6 +37,9 @@ nextflow_process { test("sarscov2 single-end [fastqc] [config]") { when { + params { + outdir = "$outputDir" + } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -59,6 +65,9 @@ nextflow_process { options "-stub" when { + params { + outdir = "$outputDir" + } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) From 1782849d4ba40d4240d6f8299aa4f782e9aee6ec Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 31 Jan 2024 01:18:01 +0000 Subject: [PATCH 78/89] trying something else --- conf/test.config | 2 -- modules/nf-core/fastqc/tests/main.nf.test | 21 --------------------- modules/nf-core/multiqc/tests/main.nf.test | 9 --------- 3 files changed, 32 deletions(-) diff --git a/conf/test.config b/conf/test.config index 3a71f83..e472172 100644 --- a/conf/test.config +++ b/conf/test.config @@ -19,5 +19,3 @@ params { max_memory = '6.GB' max_time = '6.h' } - -includeConfig './test_data.config' diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 58fc73d..1f21c66 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -11,9 +11,6 @@ nextflow_process { test("sarscov2 single-end [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([ @@ -44,9 +41,6 @@ nextflow_process { test("sarscov2 paired-end [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([ @@ -77,9 +71,6 @@ nextflow_process { test("sarscov2 interleaved [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([ @@ -106,9 +97,6 @@ nextflow_process { test("sarscov2 paired-end [bam]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([ @@ -135,9 +123,6 @@ nextflow_process { test("sarscov2 multiple [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([ @@ -176,9 +161,6 @@ nextflow_process { test("sarscov2 custom_prefix") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([ @@ -207,9 +189,6 @@ nextflow_process { options "-stub" when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([ diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index 9a0207b..d0438ed 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -10,9 +10,6 @@ nextflow_process { test("sarscov2 single-end [fastqc]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -37,9 +34,6 @@ nextflow_process { test("sarscov2 single-end [fastqc] [config]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -65,9 +59,6 @@ nextflow_process { options "-stub" when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) From 458ba933ae1466328a5362685cae2727c991c372 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 31 Jan 2024 01:22:24 +0000 Subject: [PATCH 79/89] trying again --- nextflow.config | 2 ++ 1 file changed, 2 insertions(+) diff --git a/nextflow.config b/nextflow.config index 30b2d03..6a81a32 100644 --- a/nextflow.config +++ b/nextflow.config @@ -115,6 +115,8 @@ params { // Load base.config by default for all pipelines includeConfig 'conf/base.config' +includeConfig 'conf/test_data.config + // Load ref_databases.config for reference taxonomy includeConfig 'conf/ref_databases.config' From 58a7aed686f25992d41293fae6bef0f215ae3202 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 31 Jan 2024 01:24:30 +0000 Subject: [PATCH 80/89] trying again --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 6a81a32..4f212c8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -115,7 +115,7 @@ params { // Load base.config by default for all pipelines includeConfig 'conf/base.config' -includeConfig 'conf/test_data.config +includeConfig 'conf/test_data.config' // Load ref_databases.config for reference taxonomy includeConfig 'conf/ref_databases.config' From a40b446d4b0c54eb877455611d336e65bab7fb85 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 31 Jan 2024 01:29:15 +0000 Subject: [PATCH 81/89] trying again --- conf/test.config | 2 ++ nextflow.config | 2 -- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/conf/test.config b/conf/test.config index e472172..a1c6e88 100644 --- a/conf/test.config +++ b/conf/test.config @@ -19,3 +19,5 @@ params { max_memory = '6.GB' max_time = '6.h' } + +includeConfig 'conf/test_data.config' diff --git a/nextflow.config b/nextflow.config index 4f212c8..30b2d03 100644 --- a/nextflow.config +++ b/nextflow.config @@ -115,8 +115,6 @@ params { // Load base.config by default for all pipelines includeConfig 'conf/base.config' -includeConfig 'conf/test_data.config' - // Load ref_databases.config for reference taxonomy includeConfig 'conf/ref_databases.config' From 81708fb2350c2db978235d172b8b1d848e6c8b66 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 31 Jan 2024 01:39:45 +0000 Subject: [PATCH 82/89] removed includeConfig again --- conf/test.config | 2 -- 1 file changed, 2 deletions(-) diff --git a/conf/test.config b/conf/test.config index a1c6e88..e472172 100644 --- a/conf/test.config +++ b/conf/test.config @@ -19,5 +19,3 @@ params { max_memory = '6.GB' max_time = '6.h' } - -includeConfig 'conf/test_data.config' From ea3982cedb57bcf217ec95b74ed19219e76a8637 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Wed, 31 Jan 2024 02:09:29 +0000 Subject: [PATCH 83/89] trying something else --- modules/nf-core/fastqc/tests/tags.yml | 4 ++-- modules/nf-core/multiqc/tests/tags.yml | 4 ++-- workflows/readsimulator/tests/tags.yml | 4 ++++ 3 files changed, 8 insertions(+), 4 deletions(-) diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml index 7834294..26551dd 100644 --- a/modules/nf-core/fastqc/tests/tags.yml +++ b/modules/nf-core/fastqc/tests/tags.yml @@ -1,2 +1,2 @@ -fastqc: - - modules/nf-core/fastqc/** +#fastqc: +# - modules/nf-core/fastqc/** diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml index bea6c0d..7e1be06 100644 --- a/modules/nf-core/multiqc/tests/tags.yml +++ b/modules/nf-core/multiqc/tests/tags.yml @@ -1,2 +1,2 @@ -multiqc: - - modules/nf-core/multiqc/** +#multiqc: +# - modules/nf-core/multiqc/** diff --git a/workflows/readsimulator/tests/tags.yml b/workflows/readsimulator/tests/tags.yml index cd12567..393123b 100644 --- a/workflows/readsimulator/tests/tags.yml +++ b/workflows/readsimulator/tests/tags.yml @@ -2,3 +2,7 @@ readsimulator_test_amplicon: - ./** readsimulator_test_wholegenome: - ./** +readsimulator_test_metagenome: + - ./** +readsimulator_test_target_capture: + - ./** From 27a97b18fff8fa8a30cf45351bf0a8053b303135 Mon Sep 17 00:00:00 2001 From: "Carson J. Miller" Date: Fri, 2 Feb 2024 15:44:07 +0000 Subject: [PATCH 84/89] Fixed linting issues --- nextflow.config | 5 ++++- nextflow_schema.json | 4 ++-- 2 files changed, 6 insertions(+), 3 deletions(-) diff --git a/nextflow.config b/nextflow.config index d588772..b15b922 100644 --- a/nextflow.config +++ b/nextflow.config @@ -112,8 +112,11 @@ params { validate_params = true } +// Load test_data.config for for using test-datasets +includeConfig 'conf/base.config' + // Load base.config by default for all pipelines -includeConfig 'conf/test_data.config' +includeConfig 'conf/base.config' // Load ref_databases.config for reference taxonomy includeConfig 'conf/ref_databases.config' diff --git a/nextflow_schema.json b/nextflow_schema.json index 2eb5a22..90ffab0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -158,7 +158,7 @@ }, "target_capture_fshape": { "type": "number", - "default": 6, + "default": 6.0, "description": "Shape parameter of the fragment size distribution." }, "target_capture_smedian": { @@ -168,7 +168,7 @@ }, "target_capture_sshape": { "type": "number", - "default": 6, + "default": 6.0, "description": "Shape parameter of the fragment size distribution." }, "target_capture_tmedian": { From 0daaaa668fbf63adeb53e16da99afd982e69b0ad Mon Sep 17 00:00:00 2001 From: "Carson J. Miller" Date: Fri, 2 Feb 2024 18:26:54 +0000 Subject: [PATCH 85/89] Updated nf-tests test data --- .../meta/assets/adaptivecard.json | 67 + .../meta/assets/email_template.html | 53 + .../meta/assets/email_template.txt | 39 + .../assets/methods_description_template.yml | 29 + .../meta/assets/multiqc_config.yml | 15 + .../nf-core-readsimulator_logo_light.png | Bin 0 -> 98327 bytes .../meta/assets/samplesheet.csv | 3 + .../meta/assets/schema_input.json | 26 + .../meta/assets/sendmail_template.txt | 53 + .../meta/assets/slackreport.json | 34 + .../meta/bin/check_samplesheet.py | 259 + .../meta/lib/NfcoreTemplate.groovy | 356 + .../meta/lib/NftestUtils.groovy | 38 + .../meta/lib/Utils.groovy | 47 + .../meta/lib/WorkflowMain.groovy | 77 + .../meta/lib/WorkflowReadsimulator.groovy | 131 + .../meta/assets/adaptivecard.json | 67 + .../meta/assets/email_template.html | 53 + .../meta/assets/email_template.txt | 39 + .../assets/methods_description_template.yml | 29 + 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mode 100755 .nf-test/tests/ffd6c523c67f7dfcb71f6bbce2a60d22/meta/bin/check_samplesheet.py create mode 100755 .nf-test/tests/ffd6c523c67f7dfcb71f6bbce2a60d22/meta/lib/NfcoreTemplate.groovy create mode 100644 .nf-test/tests/ffd6c523c67f7dfcb71f6bbce2a60d22/meta/lib/NftestUtils.groovy create mode 100644 .nf-test/tests/ffd6c523c67f7dfcb71f6bbce2a60d22/meta/lib/Utils.groovy create mode 100755 .nf-test/tests/ffd6c523c67f7dfcb71f6bbce2a60d22/meta/lib/WorkflowMain.groovy create mode 100755 .nf-test/tests/ffd6c523c67f7dfcb71f6bbce2a60d22/meta/lib/WorkflowReadsimulator.groovy create mode 100644 tests/nextflow.config diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/adaptivecard.json b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/adaptivecard.json new file mode 100644 index 0000000..dbf3df9 --- /dev/null +++ b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/adaptivecard.json @@ -0,0 +1,67 @@ +{ + "type": "message", + "attachments": [ + { + "contentType": "application/vnd.microsoft.card.adaptive", + "contentUrl": null, + "content": { + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "msteams": { + "width": "Full" + }, + "type": "AdaptiveCard", + "version": "1.2", + "body": [ + { + "type": "TextBlock", + "size": "Large", + "weight": "Bolder", + "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", + "text": "nf-core/readsimulator v${version} - ${runName}", + "wrap": true + }, + { + "type": "TextBlock", + "spacing": "None", + "text": "Completed at ${dateComplete} (duration: ${duration})", + "isSubtle": true, + "wrap": true + }, + { + "type": "TextBlock", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors. The full error message was: ${errorReport}.<% } %>", + "wrap": true + }, + { + "type": "TextBlock", + "text": "The command used to launch the workflow was as follows:", + "wrap": true + }, + { + "type": "TextBlock", + "text": "${commandLine}", + "isSubtle": true, + "wrap": true + } + ], + "actions": [ + { + "type": "Action.ShowCard", + "title": "Pipeline Configuration", + "card": { + "type": "AdaptiveCard", + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "body": [ + { + "type": "FactSet", + "facts": [<% out << summary.collect{ k,v -> "{\"title\": \"$k\", \"value\" : \"$v\"}"}.join(",\n") %> + ] + } + ] + } + } + ] + } + } + ] +} diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/email_template.html b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/email_template.html new file mode 100644 index 0000000..f2bc049 --- /dev/null +++ b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/email_template.html @@ -0,0 +1,53 @@ + + + + + + + + nf-core/readsimulator Pipeline Report + + +
    + + + +

    nf-core/readsimulator ${version}

    +

    Run Name: $runName

    + +<% if (!success){ + out << """ +
    +

    nf-core/readsimulator execution completed unsuccessfully!

    +

    The exit status of the task that caused the workflow execution to fail was: $exitStatus.

    +

    The full error message was:

    + 
    ${errorReport}
    +
    + """ +} else { + out << """ +
    + nf-core/readsimulator execution completed successfully! +
    + """ +} +%> + +

    The workflow was completed at $dateComplete (duration: $duration)

    +

    The command used to launch the workflow was as follows:

    +
    $commandLine
    + +

    Pipeline Configuration:

    +
    Process Name \\", + " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    + + <% out << summary.collect{ k,v -> "" }.join("\n") %> + +
    $k
    $v
    + +

    nf-core/readsimulator

    +

    https://github.com/nf-core/readsimulator

    + + + + + diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/email_template.txt b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/email_template.txt new file mode 100644 index 0000000..6ca5507 --- /dev/null +++ b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/email_template.txt @@ -0,0 +1,39 @@ +---------------------------------------------------- + ,--./,-. + ___ __ __ __ ___ /,-._.--~\\ + |\\ | |__ __ / ` / \\ |__) |__ } { + | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, + `._,._,' + nf-core/readsimulator ${version} +---------------------------------------------------- +Run Name: $runName + +<% if (success){ + out << "## nf-core/readsimulator execution completed successfully! ##" +} else { + out << """#################################################### +## nf-core/readsimulator execution completed unsuccessfully! ## +#################################################### +The exit status of the task that caused the workflow execution to fail was: $exitStatus. +The full error message was: + +${errorReport} +""" +} %> + + +The workflow was completed at $dateComplete (duration: $duration) + +The command used to launch the workflow was as follows: + + $commandLine + + + +Pipeline Configuration: +----------------------- +<% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> + +-- +nf-core/readsimulator +https://github.com/nf-core/readsimulator diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/methods_description_template.yml b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/methods_description_template.yml new file mode 100644 index 0000000..6119677 --- /dev/null +++ b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/methods_description_template.yml @@ -0,0 +1,29 @@ +id: "nf-core-readsimulator-methods-description" +description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." +section_name: "nf-core/readsimulator Methods Description" +section_href: "https://github.com/nf-core/readsimulator" +plot_type: "html" +## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline +## You inject any metadata in the Nextflow '${workflow}' object +data: | +

    Methods

    +

    Data was processed using nf-core/readsimulator v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

    +

    The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

    + 
    ${workflow.commandLine}
    +

    ${tool_citations}

    +

    References

    +
      +
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
      • +
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
      • +
    • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
      • +
    • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
      • + ${tool_bibliography} +
    +
    +
    Notes:
    +
      + ${nodoi_text} +
    • The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
      • +
    • You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.
      • +
    +
    diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/multiqc_config.yml b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/multiqc_config.yml new file mode 100644 index 0000000..7d3650d --- /dev/null +++ b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/multiqc_config.yml @@ -0,0 +1,15 @@ +report_comment: > + + This report has been generated by the nf-core/readsimulator + analysis pipeline. For information about how to interpret these results, please see the + documentation. + +report_section_order: + "nf-core-readsimulator-methods-description": + order: -1000 + software_versions: + order: -1001 + "nf-core-readsimulator-summary": + order: -1002 + +export_plots: true diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/nf-core-readsimulator_logo_light.png b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/nf-core-readsimulator_logo_light.png new file mode 100644 index 0000000000000000000000000000000000000000..6dec24128e0e90368c9f0f2a9d691df15a1298a2 GIT binary patch literal 98327 zcmeFY_dnbH_dl*zhqhXJwMt9TVQ;l+w?&CrD`wDIwTl?BuWIW;(Z+~Sv}Vm9R?N~W zAx7*Ks#a`b#0sBh^!2)~*X{eu_aAsaf50uCk8{rBJkGd3&N+#As-t%KBFjY@8k)-> zbrpRYn)8P=G^gp$p94M#9gQrap*a=csI2@Hq^x}Vsi(WWql+C4jo(M_50e66dN*2? zr0$O0wWxksDp4%e+5hVaQo;!&ZdW}rLD3(pt6$^97`Yk8S8pu6Y78}>HRHR><=Zal z5oXz4TU2S=y&%&)AJ`2`>w?jn+%@IOmG-}b5P30W{Z)no@=~hNy7kp%S~f9N<5qk7 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charset=utf-8 + +$email_html + +--nfcoremimeboundary +Content-Type: image/png;name="nf-core-readsimulator_logo.png" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: inline; filename="nf-core-readsimulator_logo_light.png" + +<% out << new File("$projectDir/assets/nf-core-readsimulator_logo_light.png"). + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' ) %> + +<% +if (mqcFile){ +def mqcFileObj = new File("$mqcFile") +if (mqcFileObj.length() < mqcMaxSize){ +out << """ +--nfcoremimeboundary +Content-Type: text/html; name=\"multiqc_report\" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" + +${mqcFileObj. + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} +""" +}} +%> + +--nfcoremimeboundary-- diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/slackreport.json b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/slackreport.json new file mode 100644 index 0000000..e9d5f8a --- /dev/null +++ b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/assets/slackreport.json @@ -0,0 +1,34 @@ +{ + "attachments": [ + { + "fallback": "Plain-text summary of the attachment.", + "color": "<% if (success) { %>good<% } else { %>danger<%} %>", + "author_name": "nf-core/readsimulator ${version} - ${runName}", + "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", + "fields": [ + { + "title": "Command used to launch the workflow", + "value": "```${commandLine}```", + "short": false + } + <% + if (!success) { %> + , + { + "title": "Full error message", + "value": "```${errorReport}```", + "short": false + }, + { + "title": "Pipeline configuration", + "value": "<% out << summary.collect{ k,v -> k == "hook_url" ? "_${k}_: (_hidden_)" : ( ( v.class.toString().contains('Path') || ( v.class.toString().contains('String') && v.contains('/') ) ) ? "_${k}_: `${v}`" : (v.class.toString().contains('DateTime') ? ("_${k}_: " + v.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM))) : "_${k}_: ${v}") ) }.join(",\n") %>", + "short": false + } + <% } + %> + ], + "footer": "Completed at <% out << dateComplete.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM)) %> (duration: ${duration})" + } + ] +} diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/bin/check_samplesheet.py b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/bin/check_samplesheet.py new file mode 100755 index 0000000..4a758fe --- /dev/null +++ b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/bin/check_samplesheet.py @@ -0,0 +1,259 @@ +#!/usr/bin/env python + + +"""Provide a command line tool to validate and transform tabular samplesheets.""" + + +import argparse +import csv +import logging +import sys +from collections import Counter +from pathlib import Path + +logger = logging.getLogger() + + +class RowChecker: + """ + Define a service that can validate and transform each given row. + + Attributes: + modified (list): A list of dicts, where each dict corresponds to a previously + validated and transformed row. The order of rows is maintained. + + """ + + VALID_FORMATS = ( + ".fq.gz", + ".fastq.gz", + ) + + def __init__( + self, + sample_col="sample", + first_col="fastq_1", + second_col="fastq_2", + single_col="single_end", + **kwargs, + ): + """ + Initialize the row checker with the expected column names. + + Args: + sample_col (str): The name of the column that contains the sample name + (default "sample"). + first_col (str): The name of the column that contains the first (or only) + FASTQ file path (default "fastq_1"). + second_col (str): The name of the column that contains the second (if any) + FASTQ file path (default "fastq_2"). + single_col (str): The name of the new column that will be inserted and + records whether the sample contains single- or paired-end sequencing + reads (default "single_end"). + + """ + super().__init__(**kwargs) + self._sample_col = sample_col + self._first_col = first_col + self._second_col = second_col + self._single_col = single_col + self._seen = set() + self.modified = [] + + def validate_and_transform(self, row): + """ + Perform all validations on the given row and insert the read pairing status. + + Args: + row (dict): A mapping from column headers (keys) to elements of that row + (values). + + """ + self._validate_sample(row) + self._validate_first(row) + self._validate_second(row) + self._validate_pair(row) + self._seen.add((row[self._sample_col], row[self._first_col])) + self.modified.append(row) + + def _validate_sample(self, row): + """Assert that the sample name exists and convert spaces to underscores.""" + if len(row[self._sample_col]) <= 0: + raise AssertionError("Sample input is required.") + # Sanitize samples slightly. + row[self._sample_col] = row[self._sample_col].replace(" ", "_") + + def _validate_first(self, row): + """Assert that the first FASTQ entry is non-empty and has the right format.""" + if len(row[self._first_col]) <= 0: + raise AssertionError("At least the first FASTQ file is required.") + self._validate_fastq_format(row[self._first_col]) + + def _validate_second(self, row): + """Assert that the second FASTQ entry has the right format if it exists.""" + if len(row[self._second_col]) > 0: + self._validate_fastq_format(row[self._second_col]) + + def _validate_pair(self, row): + """Assert that read pairs have the same file extension. Report pair status.""" + if row[self._first_col] and row[self._second_col]: + row[self._single_col] = False + first_col_suffix = Path(row[self._first_col]).suffixes[-2:] + second_col_suffix = Path(row[self._second_col]).suffixes[-2:] + if first_col_suffix != second_col_suffix: + raise AssertionError("FASTQ pairs must have the same file extensions.") + else: + row[self._single_col] = True + + def _validate_fastq_format(self, filename): + """Assert that a given filename has one of the expected FASTQ extensions.""" + if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): + raise AssertionError( + f"The FASTQ file has an unrecognized extension: {filename}\n" + f"It should be one of: {', '.join(self.VALID_FORMATS)}" + ) + + def validate_unique_samples(self): + """ + Assert that the combination of sample name and FASTQ filename is unique. + + In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the + number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. + + """ + if len(self._seen) != len(self.modified): + raise AssertionError("The pair of sample name and FASTQ must be unique.") + seen = Counter() + for row in self.modified: + sample = row[self._sample_col] + seen[sample] += 1 + row[self._sample_col] = f"{sample}_T{seen[sample]}" + + +def read_head(handle, num_lines=10): + """Read the specified number of lines from the current position in the file.""" + lines = [] + for idx, line in enumerate(handle): + if idx == num_lines: + break + lines.append(line) + return "".join(lines) + + +def sniff_format(handle): + """ + Detect the tabular format. + + Args: + handle (text file): A handle to a `text file`_ object. The read position is + expected to be at the beginning (index 0). + + Returns: + csv.Dialect: The detected tabular format. + + .. _text file: + https://docs.python.org/3/glossary.html#term-text-file + + """ + peek = read_head(handle) + handle.seek(0) + sniffer = csv.Sniffer() + dialect = sniffer.sniff(peek) + return dialect + + +def check_samplesheet(file_in, file_out): + """ + Check that the tabular samplesheet has the structure expected by nf-core pipelines. + + Validate the general shape of the table, expected columns, and each row. Also add + an additional column which records whether one or two FASTQ reads were found. + + Args: + file_in (pathlib.Path): The given tabular samplesheet. The format can be either + CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. + file_out (pathlib.Path): Where the validated and transformed samplesheet should + be created; always in CSV format. + + Example: + This function checks that the samplesheet follows the following structure, + see also the `viral recon samplesheet`_:: + + sample,fastq_1,fastq_2 + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + + .. _viral recon samplesheet: + https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + + """ + required_columns = {"sample", "fastq_1", "fastq_2"} + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_in.open(newline="") as in_handle: + reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) + # Validate the existence of the expected header columns. + if not required_columns.issubset(reader.fieldnames): + req_cols = ", ".join(required_columns) + logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") + sys.exit(1) + # Validate each row. + checker = RowChecker() + for i, row in enumerate(reader): + try: + checker.validate_and_transform(row) + except AssertionError as error: + logger.critical(f"{str(error)} On line {i + 2}.") + sys.exit(1) + checker.validate_unique_samples() + header = list(reader.fieldnames) + header.insert(1, "single_end") + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_out.open(mode="w", newline="") as out_handle: + writer = csv.DictWriter(out_handle, header, delimiter=",") + writer.writeheader() + for row in checker.modified: + writer.writerow(row) + + +def parse_args(argv=None): + """Define and immediately parse command line arguments.""" + parser = argparse.ArgumentParser( + description="Validate and transform a tabular samplesheet.", + epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", + ) + parser.add_argument( + "file_in", + metavar="FILE_IN", + type=Path, + help="Tabular input samplesheet in CSV or TSV format.", + ) + parser.add_argument( + "file_out", + metavar="FILE_OUT", + type=Path, + help="Transformed output samplesheet in CSV format.", + ) + parser.add_argument( + "-l", + "--log-level", + help="The desired log level (default WARNING).", + choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), + default="WARNING", + ) + return parser.parse_args(argv) + + +def main(argv=None): + """Coordinate argument parsing and program execution.""" + args = parse_args(argv) + logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") + if not args.file_in.is_file(): + logger.error(f"The given input file {args.file_in} was not found!") + sys.exit(2) + args.file_out.parent.mkdir(parents=True, exist_ok=True) + check_samplesheet(args.file_in, args.file_out) + + +if __name__ == "__main__": + sys.exit(main()) diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/NfcoreTemplate.groovy b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/NfcoreTemplate.groovy new file mode 100755 index 0000000..e248e4c --- /dev/null +++ b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/NfcoreTemplate.groovy @@ -0,0 +1,356 @@ +// +// This file holds several functions used within the nf-core pipeline template. +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +class NfcoreTemplate { + + // + // Check AWS Batch related parameters have been specified correctly + // + public static void awsBatch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + // Check params.awsqueue and params.awsregion have been set if running on AWSBatch + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + } + } + + // + // Warn if a -profile or Nextflow config has not been provided to run the pipeline + // + public static void checkConfigProvided(workflow, log) { + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + } + } + + // + // Generate version string + // + public static String version(workflow) { + String version_string = "" + + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string + } + + // + // Construct and send completion email + // + public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = NfcoreTemplate.version(workflow) + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() + } + + // + // Construct and send a notification to a web server as JSON + // e.g. Microsoft Teams and Slack + // + public static void IM_notification(workflow, params, summary_params, projectDir, log) { + def hook_url = params.hook_url + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = NfcoreTemplate.version(workflow) + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("$projectDir/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } + } + + // + // Dump pipeline parameters in a json file + // + public static void dump_parameters(workflow, params) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() + } + + // + // Print pipeline summary on completion + // + public static void summary(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } + + // + // ANSII Colours used for terminal logging + // + public static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + // + // Does what is says on the tin + // + public static String dashedLine(monochrome_logs) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + // + // nf-core logo + // + public static String logo(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String workflow_version = NfcoreTemplate.version(workflow) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/NftestUtils.groovy b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/NftestUtils.groovy new file mode 100644 index 0000000..ce6fc8b --- /dev/null +++ b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/NftestUtils.groovy @@ -0,0 +1,38 @@ +// Helper functions for pipeline tests + +class NftestUtils { + + // Function to remove Nextflow version from software_versions.yml + public static String removeNextflowVersion(outputDir) { + def softwareVersions = path("$outputDir/pipeline_info/software_versions.yml").yaml + if (softwareVersions.containsKey("Workflow")) { + softwareVersions.Workflow.remove("Nextflow") + } + return softwareVersions + } + + // Function to filter lines from a file and return a new file + public static File filterLines(String inFilePath, int linesToSkip) { + if (linesToSkip >= 0) { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lineCount = 0 + inputFile.eachLine { line -> + lineCount++ + if (lineCount > linesToSkip) { + outputFile.append(line + '\n') + } + } + return outputFile + } else { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lines = inputFile.readLines() + def totalLines = lines.size() + lines.take(totalLines + linesToSkip).each { line -> + outputFile.append(line + '\n') + } + return outputFile + } + } +} diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/Utils.groovy b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/Utils.groovy new file mode 100644 index 0000000..8d030f4 --- /dev/null +++ b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/Utils.groovy @@ -0,0 +1,47 @@ +// +// This file holds several Groovy functions that could be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml + +class Utils { + + // + // When running with -profile conda, warn if channels have not been set-up appropriately + // + public static void checkCondaChannels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } + } +} diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/WorkflowMain.groovy b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/WorkflowMain.groovy new file mode 100755 index 0000000..477ddaf --- /dev/null +++ b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/WorkflowMain.groovy @@ -0,0 +1,77 @@ +// +// This file holds several functions specific to the main.nf workflow in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow + +class WorkflowMain { + + // + // Citation string for pipeline + // + public static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + // TODO nf-core: Add Zenodo DOI for pipeline after first release + //"* The pipeline\n" + + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + + // + // Validate parameters and print summary to screen + // + public static void initialise(workflow, params, log, args) { + + // Print workflow version and exit on --version + if (params.version) { + String workflow_version = NfcoreTemplate.version(workflow) + log.info "${workflow.manifest.name} ${workflow_version}" + System.exit(0) + } + + // Check that a -profile or Nextflow config has been provided to run the pipeline + NfcoreTemplate.checkConfigProvided(workflow, log) + // Check that the profile doesn't contain spaces and doesn't end with a trailing comma + checkProfile(workflow.profile, args, log) + + // Check that conda channels are set-up correctly + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + Utils.checkCondaChannels(log) + } + + // Check AWS batch settings + NfcoreTemplate.awsBatch(workflow, params) + + // Check input has been provided + if (!params.input) { + Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") + } + } + // + // Get attribute from genome config file e.g. fasta + // + public static Object getGenomeAttribute(params, attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null + } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } +} diff --git a/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/WorkflowReadsimulator.groovy b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/WorkflowReadsimulator.groovy new file mode 100755 index 0000000..c0b9df0 --- /dev/null +++ b/.nf-test/tests/290781be4facfe0d87a1fc032669ba09/meta/lib/WorkflowReadsimulator.groovy @@ -0,0 +1,131 @@ +// +// This file holds several functions specific to the workflow/readsimulator.nf in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow +import groovy.text.SimpleTemplateEngine + +class WorkflowReadsimulator { + + // + // Check and validate parameters + // + public static void initialise(params, log) { + + genomeExistsError(params, log) + + if (!params.fasta && !params.ncbidownload_accessions && !params.ncbidownload_taxids) { + Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file. Alternatively, '--ncbidownload_accessions accessions.txt' or '--ncbidownload_taxids taxids.txt' can be used." + } + } + + // + // Get workflow summary for MultiQC + // + public static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } + + // + // Generate methods description for MultiQC + // + + public static String toolCitationText(params) { + def citation_text = [ + "Tools used in the workflow included:", + params["amplicon"] ? [ + "CRABS (Jeunen et al. 2022),", + "ART (Huang et al. 2012)," + ].join(' ').trim() : "", + params["target_capture"] ? [ + "Bowtie2 (Langmead et al. 2012),", + "Samtools (Danecek et al. 2021),", + "CapSim (Cao et al. 2018)," + ].join(' ').trim() : "", + params["metagenome"] ? + "InSilicoSeq (Gourlé et al. 2018)," : "", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text + } + + public static String toolBibliographyText(params) { + def reference_text = [ + params["amplicon"] ? [ + "
  • Jeunen, G.-J., Dowle, E., Edgecombe, J., von Ammon, U., Gemmell, N. J., & Cross, H. (2022). crabs—A software program to generate curated reference databases for metabarcoding sequencing data. Molecular Ecology Resources, 00, 1– 14. https://doi.org/10.1111/1755-0998.13741
    • ", + "
  • Weichun Huang, Leping Li, Jason R. Myers, Gabor T. Marth, ART: a next-generation sequencing read simulator, Bioinformatics, Volume 28, Issue 4, February 2012, Pages 593–594, https://doi.org/10.1093/bioinformatics/btr708
    • " + ].join(' ').trim() : "", + params["target_capture"] ? [ + "
  • Langmead, B., Salzberg, S. Fast gapped-read alignment with Bowtie 2. Nat Methods 9, 357–359 (2012). https://doi.org/10.1038/nmeth.1923
    • ", + "
  • Twelve years of SAMtools and BCFtools. Petr Danecek, James K Bonfield, Jennifer Liddle, John Marshall, Valeriu Ohan, Martin O Pollard, Andrew Whitwham, Thomas Keane, Shane A McCarthy, Robert M Davies, Heng Li. GigaScience, Volume 10, Issue 2, February 2021, giab008, https://doi.org/10.1093/gigascience/giab008
    • ", + "
  • Minh Duc Cao, Devika Ganesamoorthy, Chenxi Zhou, Lachlan J M Coin, Simulating the dynamics of targeted capture sequencing with CapSim, Bioinformatics, Volume 34, Issue 5, March 2018, Pages 873–874, https://doi.org/10.1093/bioinformatics/btx691
    • " + ].join(' ').trim() : "", + params["metagenome"] ? + "
  • Gourlé H, Karlsson-Lindsjö O, Hayer J and Bongcam+Rudloff E, Simulating Illumina data with InSilicoSeq. Bioinformatics (2018) doi:10.1093/bioinformatics/bty630
    • " : "", + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text + } + + public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = run_workflow.toMap() + meta["manifest_map"] = run_workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + meta["tool_bibliography"] = toolBibliographyText(params) + + + def methods_text = mqc_methods_yaml.text + + def engine = new SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html + } + + // + // Exit pipeline if incorrect --genome key provided + // + private static void genomeExistsError(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + Nextflow.error(error_string) + } + } +} diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/adaptivecard.json b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/adaptivecard.json new file mode 100644 index 0000000..dbf3df9 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/adaptivecard.json @@ -0,0 +1,67 @@ +{ + "type": "message", + "attachments": [ + { + "contentType": "application/vnd.microsoft.card.adaptive", + "contentUrl": null, + "content": { + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "msteams": { + "width": "Full" + }, + "type": "AdaptiveCard", + "version": "1.2", + "body": [ + { + "type": "TextBlock", + "size": "Large", + "weight": "Bolder", + "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", + "text": "nf-core/readsimulator v${version} - ${runName}", + "wrap": true + }, + { + "type": "TextBlock", + "spacing": "None", + "text": "Completed at ${dateComplete} (duration: ${duration})", + "isSubtle": true, + "wrap": true + }, + { + "type": "TextBlock", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors. The full error message was: ${errorReport}.<% } %>", + "wrap": true + }, + { + "type": "TextBlock", + "text": "The command used to launch the workflow was as follows:", + "wrap": true + }, + { + "type": "TextBlock", + "text": "${commandLine}", + "isSubtle": true, + "wrap": true + } + ], + "actions": [ + { + "type": "Action.ShowCard", + "title": "Pipeline Configuration", + "card": { + "type": "AdaptiveCard", + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "body": [ + { + "type": "FactSet", + "facts": [<% out << summary.collect{ k,v -> "{\"title\": \"$k\", \"value\" : \"$v\"}"}.join(",\n") %> + ] + } + ] + } + } + ] + } + } + ] +} diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/email_template.html b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/email_template.html new file mode 100644 index 0000000..f2bc049 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/email_template.html @@ -0,0 +1,53 @@ + + + + + + + + nf-core/readsimulator Pipeline Report + + +
    + + + +

    nf-core/readsimulator ${version}

    +

    Run Name: $runName

    + +<% if (!success){ + out << """ +
    +

    nf-core/readsimulator execution completed unsuccessfully!

    +

    The exit status of the task that caused the workflow execution to fail was: $exitStatus.

    +

    The full error message was:

    + 
    ${errorReport}
    +
    + """ +} else { + out << """ +
    + nf-core/readsimulator execution completed successfully! +
    + """ +} +%> + +

    The workflow was completed at $dateComplete (duration: $duration)

    +

    The command used to launch the workflow was as follows:

    +
    $commandLine
    + +

    Pipeline Configuration:

    + + + <% out << summary.collect{ k,v -> "" }.join("\n") %> + +
    $k
    $v
    + +

    nf-core/readsimulator

    +

    https://github.com/nf-core/readsimulator

    + +
    + + + diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/email_template.txt b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/email_template.txt new file mode 100644 index 0000000..6ca5507 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/email_template.txt @@ -0,0 +1,39 @@ +---------------------------------------------------- + ,--./,-. + ___ __ __ __ ___ /,-._.--~\\ + |\\ | |__ __ / ` / \\ |__) |__ } { + | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, + `._,._,' + nf-core/readsimulator ${version} +---------------------------------------------------- +Run Name: $runName + +<% if (success){ + out << "## nf-core/readsimulator execution completed successfully! ##" +} else { + out << """#################################################### +## nf-core/readsimulator execution completed unsuccessfully! ## +#################################################### +The exit status of the task that caused the workflow execution to fail was: $exitStatus. +The full error message was: + +${errorReport} +""" +} %> + + +The workflow was completed at $dateComplete (duration: $duration) + +The command used to launch the workflow was as follows: + + $commandLine + + + +Pipeline Configuration: +----------------------- +<% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> + +-- +nf-core/readsimulator +https://github.com/nf-core/readsimulator diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/methods_description_template.yml b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/methods_description_template.yml new file mode 100644 index 0000000..6119677 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/methods_description_template.yml @@ -0,0 +1,29 @@ +id: "nf-core-readsimulator-methods-description" +description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." +section_name: "nf-core/readsimulator Methods Description" +section_href: "https://github.com/nf-core/readsimulator" +plot_type: "html" +## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline +## You inject any metadata in the Nextflow '${workflow}' object +data: | +

    Methods

    +

    Data was processed using nf-core/readsimulator v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

    +

    The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

    + 
    ${workflow.commandLine}
    +

    ${tool_citations}

    +

    References

    +
      +
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
      • +
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
      • +
    • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
      • +
    • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
      • + ${tool_bibliography} +
    +
    +
    Notes:
    +
      + ${nodoi_text} +
    • The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
      • +
    • You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.
      • +
    +
    diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/multiqc_config.yml b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/multiqc_config.yml new file mode 100644 index 0000000..7d3650d --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/multiqc_config.yml @@ -0,0 +1,15 @@ +report_comment: > + + This report has been generated by the nf-core/readsimulator + analysis pipeline. For information about how to interpret these results, please see the + documentation. + +report_section_order: + "nf-core-readsimulator-methods-description": + order: -1000 + software_versions: + order: -1001 + "nf-core-readsimulator-summary": + order: -1002 + +export_plots: true diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/nf-core-readsimulator_logo_light.png b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/nf-core-readsimulator_logo_light.png new file mode 100644 index 0000000000000000000000000000000000000000..6dec24128e0e90368c9f0f2a9d691df15a1298a2 GIT binary patch literal 98327 zcmeFY_dnbH_dl*zhqhXJwMt9TVQ;l+w?&CrD`wDIwTl?BuWIW;(Z+~Sv}Vm9R?N~W zAx7*Ks#a`b#0sBh^!2)~*X{eu_aAsaf50uCk8{rBJkGd3&N+#As-t%KBFjY@8k)-> zbrpRYn)8P=G^gp$p94M#9gQrap*a=csI2@Hq^x}Vsi(WWql+C4jo(M_50e66dN*2? zr0$O0wWxksDp4%e+5hVaQo;!&ZdW}rLD3(pt6$^97`Yk8S8pu6Y78}>HRHR><=Zal z5oXz4TU2S=y&%&)AJ`2`>w?jn+%@IOmG-}b5P30W{Z)no@=~hNy7kp%S~f9N<5qk7 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charset=utf-8 + +$email_html + +--nfcoremimeboundary +Content-Type: image/png;name="nf-core-readsimulator_logo.png" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: inline; filename="nf-core-readsimulator_logo_light.png" + +<% out << new File("$projectDir/assets/nf-core-readsimulator_logo_light.png"). + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' ) %> + +<% +if (mqcFile){ +def mqcFileObj = new File("$mqcFile") +if (mqcFileObj.length() < mqcMaxSize){ +out << """ +--nfcoremimeboundary +Content-Type: text/html; name=\"multiqc_report\" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" + +${mqcFileObj. + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} +""" +}} +%> + +--nfcoremimeboundary-- diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/slackreport.json b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/slackreport.json new file mode 100644 index 0000000..e9d5f8a --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/assets/slackreport.json @@ -0,0 +1,34 @@ +{ + "attachments": [ + { + "fallback": "Plain-text summary of the attachment.", + "color": "<% if (success) { %>good<% } else { %>danger<%} %>", + "author_name": "nf-core/readsimulator ${version} - ${runName}", + "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", + "fields": [ + { + "title": "Command used to launch the workflow", + "value": "```${commandLine}```", + "short": false + } + <% + if (!success) { %> + , + { + "title": "Full error message", + "value": "```${errorReport}```", + "short": false + }, + { + "title": "Pipeline configuration", + "value": "<% out << summary.collect{ k,v -> k == "hook_url" ? "_${k}_: (_hidden_)" : ( ( v.class.toString().contains('Path') || ( v.class.toString().contains('String') && v.contains('/') ) ) ? "_${k}_: `${v}`" : (v.class.toString().contains('DateTime') ? ("_${k}_: " + v.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM))) : "_${k}_: ${v}") ) }.join(",\n") %>", + "short": false + } + <% } + %> + ], + "footer": "Completed at <% out << dateComplete.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM)) %> (duration: ${duration})" + } + ] +} diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/bin/check_samplesheet.py b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/bin/check_samplesheet.py new file mode 100755 index 0000000..4a758fe --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/bin/check_samplesheet.py @@ -0,0 +1,259 @@ +#!/usr/bin/env python + + +"""Provide a command line tool to validate and transform tabular samplesheets.""" + + +import argparse +import csv +import logging +import sys +from collections import Counter +from pathlib import Path + +logger = logging.getLogger() + + +class RowChecker: + """ + Define a service that can validate and transform each given row. + + Attributes: + modified (list): A list of dicts, where each dict corresponds to a previously + validated and transformed row. The order of rows is maintained. + + """ + + VALID_FORMATS = ( + ".fq.gz", + ".fastq.gz", + ) + + def __init__( + self, + sample_col="sample", + first_col="fastq_1", + second_col="fastq_2", + single_col="single_end", + **kwargs, + ): + """ + Initialize the row checker with the expected column names. + + Args: + sample_col (str): The name of the column that contains the sample name + (default "sample"). + first_col (str): The name of the column that contains the first (or only) + FASTQ file path (default "fastq_1"). + second_col (str): The name of the column that contains the second (if any) + FASTQ file path (default "fastq_2"). + single_col (str): The name of the new column that will be inserted and + records whether the sample contains single- or paired-end sequencing + reads (default "single_end"). + + """ + super().__init__(**kwargs) + self._sample_col = sample_col + self._first_col = first_col + self._second_col = second_col + self._single_col = single_col + self._seen = set() + self.modified = [] + + def validate_and_transform(self, row): + """ + Perform all validations on the given row and insert the read pairing status. + + Args: + row (dict): A mapping from column headers (keys) to elements of that row + (values). + + """ + self._validate_sample(row) + self._validate_first(row) + self._validate_second(row) + self._validate_pair(row) + self._seen.add((row[self._sample_col], row[self._first_col])) + self.modified.append(row) + + def _validate_sample(self, row): + """Assert that the sample name exists and convert spaces to underscores.""" + if len(row[self._sample_col]) <= 0: + raise AssertionError("Sample input is required.") + # Sanitize samples slightly. + row[self._sample_col] = row[self._sample_col].replace(" ", "_") + + def _validate_first(self, row): + """Assert that the first FASTQ entry is non-empty and has the right format.""" + if len(row[self._first_col]) <= 0: + raise AssertionError("At least the first FASTQ file is required.") + self._validate_fastq_format(row[self._first_col]) + + def _validate_second(self, row): + """Assert that the second FASTQ entry has the right format if it exists.""" + if len(row[self._second_col]) > 0: + self._validate_fastq_format(row[self._second_col]) + + def _validate_pair(self, row): + """Assert that read pairs have the same file extension. Report pair status.""" + if row[self._first_col] and row[self._second_col]: + row[self._single_col] = False + first_col_suffix = Path(row[self._first_col]).suffixes[-2:] + second_col_suffix = Path(row[self._second_col]).suffixes[-2:] + if first_col_suffix != second_col_suffix: + raise AssertionError("FASTQ pairs must have the same file extensions.") + else: + row[self._single_col] = True + + def _validate_fastq_format(self, filename): + """Assert that a given filename has one of the expected FASTQ extensions.""" + if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): + raise AssertionError( + f"The FASTQ file has an unrecognized extension: {filename}\n" + f"It should be one of: {', '.join(self.VALID_FORMATS)}" + ) + + def validate_unique_samples(self): + """ + Assert that the combination of sample name and FASTQ filename is unique. + + In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the + number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. + + """ + if len(self._seen) != len(self.modified): + raise AssertionError("The pair of sample name and FASTQ must be unique.") + seen = Counter() + for row in self.modified: + sample = row[self._sample_col] + seen[sample] += 1 + row[self._sample_col] = f"{sample}_T{seen[sample]}" + + +def read_head(handle, num_lines=10): + """Read the specified number of lines from the current position in the file.""" + lines = [] + for idx, line in enumerate(handle): + if idx == num_lines: + break + lines.append(line) + return "".join(lines) + + +def sniff_format(handle): + """ + Detect the tabular format. + + Args: + handle (text file): A handle to a `text file`_ object. The read position is + expected to be at the beginning (index 0). + + Returns: + csv.Dialect: The detected tabular format. + + .. _text file: + https://docs.python.org/3/glossary.html#term-text-file + + """ + peek = read_head(handle) + handle.seek(0) + sniffer = csv.Sniffer() + dialect = sniffer.sniff(peek) + return dialect + + +def check_samplesheet(file_in, file_out): + """ + Check that the tabular samplesheet has the structure expected by nf-core pipelines. + + Validate the general shape of the table, expected columns, and each row. Also add + an additional column which records whether one or two FASTQ reads were found. + + Args: + file_in (pathlib.Path): The given tabular samplesheet. The format can be either + CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. + file_out (pathlib.Path): Where the validated and transformed samplesheet should + be created; always in CSV format. + + Example: + This function checks that the samplesheet follows the following structure, + see also the `viral recon samplesheet`_:: + + sample,fastq_1,fastq_2 + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + + .. _viral recon samplesheet: + https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + + """ + required_columns = {"sample", "fastq_1", "fastq_2"} + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_in.open(newline="") as in_handle: + reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) + # Validate the existence of the expected header columns. + if not required_columns.issubset(reader.fieldnames): + req_cols = ", ".join(required_columns) + logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") + sys.exit(1) + # Validate each row. + checker = RowChecker() + for i, row in enumerate(reader): + try: + checker.validate_and_transform(row) + except AssertionError as error: + logger.critical(f"{str(error)} On line {i + 2}.") + sys.exit(1) + checker.validate_unique_samples() + header = list(reader.fieldnames) + header.insert(1, "single_end") + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_out.open(mode="w", newline="") as out_handle: + writer = csv.DictWriter(out_handle, header, delimiter=",") + writer.writeheader() + for row in checker.modified: + writer.writerow(row) + + +def parse_args(argv=None): + """Define and immediately parse command line arguments.""" + parser = argparse.ArgumentParser( + description="Validate and transform a tabular samplesheet.", + epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", + ) + parser.add_argument( + "file_in", + metavar="FILE_IN", + type=Path, + help="Tabular input samplesheet in CSV or TSV format.", + ) + parser.add_argument( + "file_out", + metavar="FILE_OUT", + type=Path, + help="Transformed output samplesheet in CSV format.", + ) + parser.add_argument( + "-l", + "--log-level", + help="The desired log level (default WARNING).", + choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), + default="WARNING", + ) + return parser.parse_args(argv) + + +def main(argv=None): + """Coordinate argument parsing and program execution.""" + args = parse_args(argv) + logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") + if not args.file_in.is_file(): + logger.error(f"The given input file {args.file_in} was not found!") + sys.exit(2) + args.file_out.parent.mkdir(parents=True, exist_ok=True) + check_samplesheet(args.file_in, args.file_out) + + +if __name__ == "__main__": + sys.exit(main()) diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/NfcoreTemplate.groovy b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/NfcoreTemplate.groovy new file mode 100755 index 0000000..e248e4c --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/NfcoreTemplate.groovy @@ -0,0 +1,356 @@ +// +// This file holds several functions used within the nf-core pipeline template. +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +class NfcoreTemplate { + + // + // Check AWS Batch related parameters have been specified correctly + // + public static void awsBatch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + // Check params.awsqueue and params.awsregion have been set if running on AWSBatch + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + } + } + + // + // Warn if a -profile or Nextflow config has not been provided to run the pipeline + // + public static void checkConfigProvided(workflow, log) { + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + } + } + + // + // Generate version string + // + public static String version(workflow) { + String version_string = "" + + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string + } + + // + // Construct and send completion email + // + public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = NfcoreTemplate.version(workflow) + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() + } + + // + // Construct and send a notification to a web server as JSON + // e.g. Microsoft Teams and Slack + // + public static void IM_notification(workflow, params, summary_params, projectDir, log) { + def hook_url = params.hook_url + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = NfcoreTemplate.version(workflow) + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("$projectDir/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } + } + + // + // Dump pipeline parameters in a json file + // + public static void dump_parameters(workflow, params) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() + } + + // + // Print pipeline summary on completion + // + public static void summary(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } + + // + // ANSII Colours used for terminal logging + // + public static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + // + // Does what is says on the tin + // + public static String dashedLine(monochrome_logs) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + // + // nf-core logo + // + public static String logo(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String workflow_version = NfcoreTemplate.version(workflow) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/NftestUtils.groovy b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/NftestUtils.groovy new file mode 100644 index 0000000..ce6fc8b --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/NftestUtils.groovy @@ -0,0 +1,38 @@ +// Helper functions for pipeline tests + +class NftestUtils { + + // Function to remove Nextflow version from software_versions.yml + public static String removeNextflowVersion(outputDir) { + def softwareVersions = path("$outputDir/pipeline_info/software_versions.yml").yaml + if (softwareVersions.containsKey("Workflow")) { + softwareVersions.Workflow.remove("Nextflow") + } + return softwareVersions + } + + // Function to filter lines from a file and return a new file + public static File filterLines(String inFilePath, int linesToSkip) { + if (linesToSkip >= 0) { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lineCount = 0 + inputFile.eachLine { line -> + lineCount++ + if (lineCount > linesToSkip) { + outputFile.append(line + '\n') + } + } + return outputFile + } else { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lines = inputFile.readLines() + def totalLines = lines.size() + lines.take(totalLines + linesToSkip).each { line -> + outputFile.append(line + '\n') + } + return outputFile + } + } +} diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/Utils.groovy b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/Utils.groovy new file mode 100644 index 0000000..8d030f4 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/Utils.groovy @@ -0,0 +1,47 @@ +// +// This file holds several Groovy functions that could be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml + +class Utils { + + // + // When running with -profile conda, warn if channels have not been set-up appropriately + // + public static void checkCondaChannels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } + } +} diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/WorkflowMain.groovy b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/WorkflowMain.groovy new file mode 100755 index 0000000..477ddaf --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/WorkflowMain.groovy @@ -0,0 +1,77 @@ +// +// This file holds several functions specific to the main.nf workflow in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow + +class WorkflowMain { + + // + // Citation string for pipeline + // + public static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + // TODO nf-core: Add Zenodo DOI for pipeline after first release + //"* The pipeline\n" + + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + + // + // Validate parameters and print summary to screen + // + public static void initialise(workflow, params, log, args) { + + // Print workflow version and exit on --version + if (params.version) { + String workflow_version = NfcoreTemplate.version(workflow) + log.info "${workflow.manifest.name} ${workflow_version}" + System.exit(0) + } + + // Check that a -profile or Nextflow config has been provided to run the pipeline + NfcoreTemplate.checkConfigProvided(workflow, log) + // Check that the profile doesn't contain spaces and doesn't end with a trailing comma + checkProfile(workflow.profile, args, log) + + // Check that conda channels are set-up correctly + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + Utils.checkCondaChannels(log) + } + + // Check AWS batch settings + NfcoreTemplate.awsBatch(workflow, params) + + // Check input has been provided + if (!params.input) { + Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") + } + } + // + // Get attribute from genome config file e.g. fasta + // + public static Object getGenomeAttribute(params, attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null + } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } +} diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/WorkflowReadsimulator.groovy b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/WorkflowReadsimulator.groovy new file mode 100755 index 0000000..c0b9df0 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib/WorkflowReadsimulator.groovy @@ -0,0 +1,131 @@ +// +// This file holds several functions specific to the workflow/readsimulator.nf in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow +import groovy.text.SimpleTemplateEngine + +class WorkflowReadsimulator { + + // + // Check and validate parameters + // + public static void initialise(params, log) { + + genomeExistsError(params, log) + + if (!params.fasta && !params.ncbidownload_accessions && !params.ncbidownload_taxids) { + Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file. Alternatively, '--ncbidownload_accessions accessions.txt' or '--ncbidownload_taxids taxids.txt' can be used." + } + } + + // + // Get workflow summary for MultiQC + // + public static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } + + // + // Generate methods description for MultiQC + // + + public static String toolCitationText(params) { + def citation_text = [ + "Tools used in the workflow included:", + params["amplicon"] ? [ + "CRABS (Jeunen et al. 2022),", + "ART (Huang et al. 2012)," + ].join(' ').trim() : "", + params["target_capture"] ? [ + "Bowtie2 (Langmead et al. 2012),", + "Samtools (Danecek et al. 2021),", + "CapSim (Cao et al. 2018)," + ].join(' ').trim() : "", + params["metagenome"] ? + "InSilicoSeq (Gourlé et al. 2018)," : "", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text + } + + public static String toolBibliographyText(params) { + def reference_text = [ + params["amplicon"] ? [ + "
  • Jeunen, G.-J., Dowle, E., Edgecombe, J., von Ammon, U., Gemmell, N. J., & Cross, H. (2022). crabs—A software program to generate curated reference databases for metabarcoding sequencing data. Molecular Ecology Resources, 00, 1– 14. https://doi.org/10.1111/1755-0998.13741
    • ", + "
  • Weichun Huang, Leping Li, Jason R. Myers, Gabor T. Marth, ART: a next-generation sequencing read simulator, Bioinformatics, Volume 28, Issue 4, February 2012, Pages 593–594, https://doi.org/10.1093/bioinformatics/btr708
    • " + ].join(' ').trim() : "", + params["target_capture"] ? [ + "
  • Langmead, B., Salzberg, S. Fast gapped-read alignment with Bowtie 2. Nat Methods 9, 357–359 (2012). https://doi.org/10.1038/nmeth.1923
    • ", + "
  • Twelve years of SAMtools and BCFtools. Petr Danecek, James K Bonfield, Jennifer Liddle, John Marshall, Valeriu Ohan, Martin O Pollard, Andrew Whitwham, Thomas Keane, Shane A McCarthy, Robert M Davies, Heng Li. GigaScience, Volume 10, Issue 2, February 2021, giab008, https://doi.org/10.1093/gigascience/giab008
    • ", + "
  • Minh Duc Cao, Devika Ganesamoorthy, Chenxi Zhou, Lachlan J M Coin, Simulating the dynamics of targeted capture sequencing with CapSim, Bioinformatics, Volume 34, Issue 5, March 2018, Pages 873–874, https://doi.org/10.1093/bioinformatics/btx691
    • " + ].join(' ').trim() : "", + params["metagenome"] ? + "
  • Gourlé H, Karlsson-Lindsjö O, Hayer J and Bongcam+Rudloff E, Simulating Illumina data with InSilicoSeq. Bioinformatics (2018) doi:10.1093/bioinformatics/bty630
    • " : "", + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text + } + + public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = run_workflow.toMap() + meta["manifest_map"] = run_workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + meta["tool_bibliography"] = toolBibliographyText(params) + + + def methods_text = mqc_methods_yaml.text + + def engine = new SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html + } + + // + // Exit pipeline if incorrect --genome key provided + // + private static void genomeExistsError(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + Nextflow.error(error_string) + } + } +} diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/mock.nf b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/mock.nf new file mode 100644 index 0000000..3f4ebab --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/mock.nf @@ -0,0 +1,92 @@ +import groovy.json.JsonGenerator +import groovy.json.JsonGenerator.Converter + +nextflow.enable.dsl=2 + +// comes from nf-test to store json files +params.nf_test_output = "" + +// include dependencies + + +// include test process +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '/workspaces/readsimulator/./modules/nf-core/custom/dumpsoftwareversions/tests/../main.nf' + +// define custom rules for JSON that will be generated. +def jsonOutput = + new JsonGenerator.Options() + .excludeNulls() // Do not include fields with value null.. + .addConverter(Path) { value -> value.toString() } // Custom converter for Path. Only filename + .build() + + +workflow { + + // run dependencies + + + // process mapping + def input = [] + + def tool1_version = ''' + TOOL1: + tool1: 0.11.9 + '''.stripIndent() + + def tool2_version = ''' + TOOL2: + tool2: 1.9 + '''.stripIndent() + + input[0] = Channel.of(tool1_version, tool2_version).collectFile() + + //---- + + //run process + CUSTOM_DUMPSOFTWAREVERSIONS(*input) + + if (CUSTOM_DUMPSOFTWAREVERSIONS.output){ + + // consumes all named output channels and stores items in a json file + for (def name in CUSTOM_DUMPSOFTWAREVERSIONS.out.getNames()) { + serializeChannel(name, CUSTOM_DUMPSOFTWAREVERSIONS.out.getProperty(name), jsonOutput) + } + + // consumes all unnamed output channels and stores items in a json file + def array = CUSTOM_DUMPSOFTWAREVERSIONS.out as Object[] + for (def i = 0; i < array.length ; i++) { + serializeChannel(i, array[i], jsonOutput) + } + + } + +} + +def serializeChannel(name, channel, jsonOutput) { + def _name = name + def list = [ ] + channel.subscribe( + onNext: { + list.add(it) + }, + onComplete: { + def map = new HashMap() + map[_name] = list + def filename = "${params.nf_test_output}/output_${_name}.json" + new File(filename).text = jsonOutput.toJson(map) + } + ) +} + + +workflow.onComplete { + + def result = [ + success: workflow.success, + exitStatus: workflow.exitStatus, + errorMessage: workflow.errorMessage, + errorReport: workflow.errorReport + ] + new File("${params.nf_test_output}/workflow.json").text = jsonOutput.toJson(result) + +} diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/nextflow.log b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/nextflow.log new file mode 100644 index 0000000..f081c65 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/nextflow.log @@ -0,0 +1,86 @@ +Feb-02 16:22:50.282 [main] DEBUG nextflow.cli.Launcher - $> nextflow -quiet -log /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/nextflow.log run /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/mock.nf -c /workspaces/readsimulator/nextflow.config -c /workspaces/readsimulator/tests/nextflow.config -params-file /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/params.json -ansi-log false -profile singularity -with-trace /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/trace.csv -w /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work +Feb-02 16:22:50.402 [main] INFO nextflow.cli.CmdRun - N E X T F L O W ~ version 23.10.1 +Feb-02 16:22:50.448 [main] DEBUG nextflow.plugin.PluginsFacade - Setting up plugin manager > mode=prod; embedded=false; plugins-dir=/home/gitpod/.nextflow/plugins; core-plugins: nf-amazon@2.1.4,nf-azure@1.3.3,nf-cloudcache@0.3.0,nf-codecommit@0.1.5,nf-console@1.0.6,nf-ga4gh@1.1.0,nf-google@1.8.3,nf-tower@1.6.3,nf-wave@1.0.1 +Feb-02 16:22:50.469 [main] INFO o.pf4j.DefaultPluginStatusProvider - Enabled plugins: [] +Feb-02 16:22:50.470 [main] INFO o.pf4j.DefaultPluginStatusProvider - Disabled plugins: [] +Feb-02 16:22:50.477 [main] INFO org.pf4j.DefaultPluginManager - PF4J version 3.4.1 in 'deployment' mode +Feb-02 16:22:50.496 [main] INFO org.pf4j.AbstractPluginManager - No plugins +Feb-02 16:22:50.521 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /workspaces/readsimulator/nextflow.config +Feb-02 16:22:50.522 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /workspaces/readsimulator/tests/nextflow.config +Feb-02 16:22:50.523 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /workspaces/readsimulator/nextflow.config +Feb-02 16:22:50.523 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /workspaces/readsimulator/tests/nextflow.config +Feb-02 16:22:50.563 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity` +Feb-02 16:22:53.816 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity` +Feb-02 16:22:55.031 [main] DEBUG nextflow.config.ConfigBuilder - Available config profiles: [cfc_dev, uzl_omics, ifb_core, denbi_qbic, alice, mjolnir_globe, uppmax, incliva, ilifu, uge, rosalind_uge, lugh, unibe_ibu, vai, czbiohub_aws, jax, ccga_med, scw, tigem, tubingen_apg, google, apollo, ipop_up, pdc_kth, googlels, daisybio, eddie, medair, biowulf, apptainer, bi, bigpurple, adcra, cedars, pawsey_setonix, vsc_kul_uhasselt, pawsey_nimbus, ucl_myriad, utd_ganymede, charliecloud, icr_davros, ceres, munin, arm, rosalind, hasta, cfc, uzh, ebi_codon_slurm, ebc, ku_sund_dangpu, ccga_dx, crick, marvin, biohpc_gen, shifter, mana, mamba, unc_lccc, wehi, awsbatch, wustl_htcf, imperial, maestro, software_license, genotoul, nci_gadi, abims, janelia, nu_genomics, googlebatch, oist, sahmri, mpcdf, leicester, vsc_ugent, create, sage, cambridge, jex, podman, ebi_codon, cheaha, xanadu, nyu_hpc, test, computerome, ucd_sonic, seg_globe, sanger, dkfz, pasteur, ethz_euler, test_full, imb, tuos_stanage, azurebatch, hki, crukmi, csiro_petrichor, docker, engaging, gis, hypatia, psmn, eva, nygc, fgcz, conda, crg, singularity, self_hosted_runner, uw_hyak_pedslabs, utd_sysbio, debug, genouest, cbe, phoenix, gitpod, seawulf, uod_hpc, fub_curta, uct_hpc, aws_tower, binac] +Feb-02 16:22:55.112 [main] DEBUG nextflow.cli.CmdRun - Applied DSL=2 from script declararion +Feb-02 16:22:55.133 [main] INFO nextflow.cli.CmdRun - Launching `/workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/mock.nf` [focused_visvesvaraya] DSL2 - revision: a50b74b347 +Feb-02 16:22:55.134 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins declared=[nf-validation@1.1.3] +Feb-02 16:22:55.134 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins default=[] +Feb-02 16:22:55.134 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins resolved requirement=[nf-validation@1.1.3] +Feb-02 16:22:55.134 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-validation version: 1.1.3 +Feb-02 16:22:55.141 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-validation@1.1.3' resolved +Feb-02 16:22:55.141 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-validation@1.1.3' +Feb-02 16:22:55.150 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-validation@1.1.3 +Feb-02 16:22:55.160 [main] DEBUG n.secret.LocalSecretsProvider - Secrets store: /home/gitpod/.nextflow/secrets/store.json +Feb-02 16:22:55.166 [main] DEBUG nextflow.secret.SecretsLoader - Discovered secrets providers: [nextflow.secret.LocalSecretsProvider@3e38c472] - activable => nextflow.secret.LocalSecretsProvider@3e38c472 +Feb-02 16:22:55.253 [main] DEBUG nextflow.Session - Session UUID: f8bce8cc-8801-459f-9944-9de914f53a64 +Feb-02 16:22:55.253 [main] DEBUG nextflow.Session - Run name: focused_visvesvaraya +Feb-02 16:22:55.254 [main] DEBUG nextflow.Session - Executor pool size: 8 +Feb-02 16:22:55.263 [main] DEBUG nextflow.file.FilePorter - File porter settings maxRetries=3; maxTransfers=50; pollTimeout=null +Feb-02 16:22:55.268 [main] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'FileTransfer' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:22:55.296 [main] DEBUG nextflow.cli.CmdRun - + Version: 23.10.1 build 5891 + Created: 12-01-2024 22:01 UTC (22:01 GMT) + System: Linux 5.15.133.1-microsoft-standard-WSL2 + Runtime: Groovy 3.0.19 on OpenJDK 64-Bit Server VM 17.0.10-internal+0-adhoc..src + Encoding: UTF-8 (UTF-8) + Process: 23901@b23599a75663 [172.17.0.3] + CPUs: 8 - Mem: 7.6 GB (293.9 MB) - Swap: 2 GB (2 GB) +Feb-02 16:22:55.325 [main] DEBUG nextflow.Session - Work-dir: /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work [ext2/ext3] +Feb-02 16:22:55.343 [main] DEBUG nextflow.executor.ExecutorFactory - Extension executors providers=[] +Feb-02 16:22:55.370 [main] DEBUG nextflow.Session - Observer factory: DefaultObserverFactory +Feb-02 16:22:55.506 [main] DEBUG nextflow.cache.CacheFactory - Using Nextflow cache factory: nextflow.cache.DefaultCacheFactory +Feb-02 16:22:55.518 [main] DEBUG nextflow.util.CustomThreadPool - Creating default thread pool > poolSize: 9; maxThreads: 1000 +Feb-02 16:22:55.617 [main] DEBUG nextflow.Session - Session start +Feb-02 16:22:55.622 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow started -- trace file: /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/trace.csv +Feb-02 16:22:55.627 [main] DEBUG nextflow.Session - Using default localLib path: /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib +Feb-02 16:22:55.632 [main] DEBUG nextflow.Session - Adding to the classpath library: /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/lib +Feb-02 16:22:55.983 [main] DEBUG nextflow.script.ScriptRunner - > Launching execution +Feb-02 16:22:56.360 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_single` matches labels `process_single` for process with name CUSTOM_DUMPSOFTWAREVERSIONS +Feb-02 16:22:56.362 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:CUSTOM_DUMPSOFTWAREVERSIONS` matches process CUSTOM_DUMPSOFTWAREVERSIONS +Feb-02 16:22:56.363 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process CUSTOM_DUMPSOFTWAREVERSIONS +Feb-02 16:22:56.369 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Feb-02 16:22:56.369 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Feb-02 16:22:56.376 [main] DEBUG nextflow.executor.Executor - [warm up] executor > local +Feb-02 16:22:56.382 [main] DEBUG n.processor.LocalPollingMonitor - Creating local task monitor for executor 'local' > cpus=8; memory=7.6 GB; capacity=8; pollInterval=100ms; dumpInterval=5m +Feb-02 16:22:56.385 [main] DEBUG n.processor.TaskPollingMonitor - >>> barrier register (monitor: local) +Feb-02 16:22:56.481 [main] DEBUG nextflow.Session - Config process names validation disabled as requested +Feb-02 16:22:56.481 [main] DEBUG nextflow.Session - Igniting dataflow network (2) +Feb-02 16:22:56.482 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > CUSTOM_DUMPSOFTWAREVERSIONS +Feb-02 16:22:56.483 [main] DEBUG nextflow.script.ScriptRunner - Parsed script files: + Script_ad258e03fe211798: /workspaces/readsimulator/./modules/nf-core/custom/dumpsoftwareversions/tests/../main.nf + Script_1b028b68302ed4d4: /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/mock.nf +Feb-02 16:22:56.483 [main] DEBUG nextflow.script.ScriptRunner - > Awaiting termination +Feb-02 16:22:56.483 [main] DEBUG nextflow.Session - Session await +Feb-02 16:22:56.650 [Actor Thread 9] DEBUG nextflow.sort.BigSort - Sort completed -- entries: 2; slices: 1; internal sort time: 0.023 s; external sort time: 0.001 s; total time: 0.024 s +Feb-02 16:22:56.660 [Actor Thread 9] DEBUG nextflow.file.FileCollector - Saved collect-files list to: /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work/collect-file/72b2aaebdb02417ce1360f46729dfb97 +Feb-02 16:22:56.671 [Actor Thread 9] DEBUG nextflow.file.FileCollector - Deleting file collector temp dir: /tmp/nxf-10028584177170524516 +Feb-02 16:22:56.810 [Actor Thread 9] WARN nextflow.container.SingularityCache - Singularity cache directory has not been defined -- Remote image will be stored in the path: /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work/singularity -- Use the environment variable NXF_SINGULARITY_CACHEDIR to specify a different location +Feb-02 16:22:56.810 [Actor Thread 9] INFO nextflow.container.SingularityCache - Pulling Singularity image https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0 [cache /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work/singularity/depot.galaxyproject.org-singularity-multiqc-1.19--pyhdfd78af_0.img] +Feb-02 16:22:57.544 [Actor Thread 9] DEBUG nextflow.container.SingularityCache - Singularity pull complete image=https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0 path=/workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work/singularity/depot.galaxyproject.org-singularity-multiqc-1.19--pyhdfd78af_0.img +Feb-02 16:22:57.672 [Task submitter] DEBUG n.executor.local.LocalTaskHandler - Launch cmd line: /bin/bash -ue .command.run +Feb-02 16:22:57.675 [Task submitter] INFO nextflow.Session - [e5/de60cd] Submitted process > CUSTOM_DUMPSOFTWAREVERSIONS (1) +Feb-02 16:22:58.448 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[id: 1; name: CUSTOM_DUMPSOFTWAREVERSIONS (1); status: COMPLETED; exit: 0; error: -; workDir: /workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work/e5/de60cd8de8c2bb231d865591c121f3] +Feb-02 16:22:58.477 [Task monitor] WARN nextflow.processor.PublishDir - Process `CUSTOM_DUMPSOFTWAREVERSIONS` publishDir path contains a variable with a null value +Feb-02 16:22:58.482 [Task monitor] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'PublishDir' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:22:58.488 [main] DEBUG nextflow.Session - Session await > all processes finished +Feb-02 16:22:58.492 [Task monitor] DEBUG n.processor.TaskPollingMonitor - <<< barrier arrives (monitor: local) - terminating tasks monitor poll loop +Feb-02 16:22:58.493 [main] DEBUG nextflow.Session - Session await > all barriers passed +Feb-02 16:22:58.496 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'PublishDir' shutdown completed (hard=false) +Feb-02 16:22:58.509 [main] DEBUG n.trace.WorkflowStatsObserver - Workflow completed > WorkflowStats[succeededCount=1; failedCount=0; ignoredCount=0; cachedCount=0; pendingCount=0; submittedCount=0; runningCount=0; retriesCount=0; abortedCount=0; succeedDuration=690ms; failedDuration=0ms; cachedDuration=0ms;loadCpus=0; loadMemory=0; peakRunning=1; peakCpus=2; peakMemory=3 GB; ] +Feb-02 16:22:58.509 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow completed -- saving trace file +Feb-02 16:22:58.513 [main] DEBUG nextflow.cache.CacheDB - Closing CacheDB done +Feb-02 16:22:58.513 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-validation@1.1.3' +Feb-02 16:22:58.513 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-validation +Feb-02 16:22:58.533 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'FileTransfer' shutdown completed (hard=false) +Feb-02 16:22:58.535 [main] DEBUG nextflow.script.ScriptRunner - > Execution complete -- Goodbye diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_0.json b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_0.json new file mode 100644 index 0000000..0acefcc --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_0.json @@ -0,0 +1 @@ +{"0":["/workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work/e5/de60cd8de8c2bb231d865591c121f3/software_versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_1.json b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_1.json new file mode 100644 index 0000000..c952832 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_1.json @@ -0,0 +1 @@ +{"1":["/workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work/e5/de60cd8de8c2bb231d865591c121f3/software_versions_mqc.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_2.json b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_2.json new file mode 100644 index 0000000..df908e2 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_2.json @@ -0,0 +1 @@ +{"2":["/workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work/e5/de60cd8de8c2bb231d865591c121f3/versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_mqc_yml.json b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_mqc_yml.json new file mode 100644 index 0000000..7da6548 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_mqc_yml.json @@ -0,0 +1 @@ +{"mqc_yml":["/workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work/e5/de60cd8de8c2bb231d865591c121f3/software_versions_mqc.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_versions.json b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_versions.json new file mode 100644 index 0000000..6265c87 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_versions.json @@ -0,0 +1 @@ +{"versions":["/workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work/e5/de60cd8de8c2bb231d865591c121f3/versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_yml.json b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_yml.json new file mode 100644 index 0000000..eadf484 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/output_yml.json @@ -0,0 +1 @@ +{"yml":["/workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/work/e5/de60cd8de8c2bb231d865591c121f3/software_versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/params.json b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/params.json new file mode 100644 index 0000000..36f4726 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/params.json @@ -0,0 +1 @@ +{"nf_test_output":"/workspaces/readsimulator/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta"} \ No newline at end of file diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/std.err b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/std.err new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/std.out b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/std.out new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/trace.csv b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/trace.csv new file mode 100644 index 0000000..ef10f66 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/trace.csv @@ -0,0 +1,2 @@ +task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar +1 e5/de60cd 24066 CUSTOM_DUMPSOFTWAREVERSIONS (1) COMPLETED 0 2024-02-02 16:22:57.674 771ms 345ms 59.7% 5.4 MB 13.8 MB 1.2 MB 1.8 KB diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/workflow.json b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/workflow.json new file mode 100644 index 0000000..7d54df0 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/meta/workflow.json @@ -0,0 +1 @@ +{"success":true,"exitStatus":0} \ No newline at end of file diff --git a/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/null/pipeline_info/software_versions.yml b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/null/pipeline_info/software_versions.yml new file mode 100644 index 0000000..2751c05 --- /dev/null +++ b/.nf-test/tests/2cb8b2270aa3c0384cc95be09fd0f275/null/pipeline_info/software_versions.yml @@ -0,0 +1,10 @@ +CUSTOM_DUMPSOFTWAREVERSIONS: + python: 3.11.7 + yaml: 5.4.1 +TOOL1: + tool1: 0.11.9 +TOOL2: + tool2: '1.9' +Workflow: + Nextflow: 23.10.1 + nf-core/readsimulator: 1.0.0 diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/adaptivecard.json b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/adaptivecard.json new file mode 100644 index 0000000..dbf3df9 --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/adaptivecard.json @@ -0,0 +1,67 @@ +{ + "type": "message", + "attachments": [ + { + "contentType": "application/vnd.microsoft.card.adaptive", + "contentUrl": null, + "content": { + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "msteams": { + "width": "Full" + }, + "type": "AdaptiveCard", + "version": "1.2", + "body": [ + { + "type": "TextBlock", + "size": "Large", + "weight": "Bolder", + "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", + "text": "nf-core/readsimulator v${version} - ${runName}", + "wrap": true + }, + { + "type": "TextBlock", + "spacing": "None", + "text": "Completed at ${dateComplete} (duration: ${duration})", + "isSubtle": true, + "wrap": true + }, + { + "type": "TextBlock", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors. The full error message was: ${errorReport}.<% } %>", + "wrap": true + }, + { + "type": "TextBlock", + "text": "The command used to launch the workflow was as follows:", + "wrap": true + }, + { + "type": "TextBlock", + "text": "${commandLine}", + "isSubtle": true, + "wrap": true + } + ], + "actions": [ + { + "type": "Action.ShowCard", + "title": "Pipeline Configuration", + "card": { + "type": "AdaptiveCard", + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "body": [ + { + "type": "FactSet", + "facts": [<% out << summary.collect{ k,v -> "{\"title\": \"$k\", \"value\" : \"$v\"}"}.join(",\n") %> + ] + } + ] + } + } + ] + } + } + ] +} diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/email_template.html b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/email_template.html new file mode 100644 index 0000000..f2bc049 --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/email_template.html @@ -0,0 +1,53 @@ + + + + + + + + nf-core/readsimulator Pipeline Report + + +
    + + + +

    nf-core/readsimulator ${version}

    +

    Run Name: $runName

    + +<% if (!success){ + out << """ +
    +

    nf-core/readsimulator execution completed unsuccessfully!

    +

    The exit status of the task that caused the workflow execution to fail was: $exitStatus.

    +

    The full error message was:

    + 
    ${errorReport}
    +
    + """ +} else { + out << """ +
    + nf-core/readsimulator execution completed successfully! +
    + """ +} +%> + +

    The workflow was completed at $dateComplete (duration: $duration)

    +

    The command used to launch the workflow was as follows:

    +
    $commandLine
    + +

    Pipeline Configuration:

    + + + <% out << summary.collect{ k,v -> "" }.join("\n") %> + +
    $k
    $v
    + +

    nf-core/readsimulator

    +

    https://github.com/nf-core/readsimulator

    + +
    + + + diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/email_template.txt b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/email_template.txt new file mode 100644 index 0000000..6ca5507 --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/email_template.txt @@ -0,0 +1,39 @@ +---------------------------------------------------- + ,--./,-. + ___ __ __ __ ___ /,-._.--~\\ + |\\ | |__ __ / ` / \\ |__) |__ } { + | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, + `._,._,' + nf-core/readsimulator ${version} +---------------------------------------------------- +Run Name: $runName + +<% if (success){ + out << "## nf-core/readsimulator execution completed successfully! ##" +} else { + out << """#################################################### +## nf-core/readsimulator execution completed unsuccessfully! ## +#################################################### +The exit status of the task that caused the workflow execution to fail was: $exitStatus. +The full error message was: + +${errorReport} +""" +} %> + + +The workflow was completed at $dateComplete (duration: $duration) + +The command used to launch the workflow was as follows: + + $commandLine + + + +Pipeline Configuration: +----------------------- +<% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> + +-- +nf-core/readsimulator +https://github.com/nf-core/readsimulator diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/methods_description_template.yml b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/methods_description_template.yml new file mode 100644 index 0000000..6119677 --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/methods_description_template.yml @@ -0,0 +1,29 @@ +id: "nf-core-readsimulator-methods-description" +description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." +section_name: "nf-core/readsimulator Methods Description" +section_href: "https://github.com/nf-core/readsimulator" +plot_type: "html" +## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline +## You inject any metadata in the Nextflow '${workflow}' object +data: | +

    Methods

    +

    Data was processed using nf-core/readsimulator v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

    +

    The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

    + 
    ${workflow.commandLine}
    +

    ${tool_citations}

    +

    References

    +
      +
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
      • +
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
      • +
    • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
      • +
    • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
      • + ${tool_bibliography} +
    +
    +
    Notes:
    +
      + ${nodoi_text} +
    • The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
      • +
    • You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.
      • +
    +
    diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/multiqc_config.yml b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/multiqc_config.yml new file mode 100644 index 0000000..7d3650d --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/multiqc_config.yml @@ -0,0 +1,15 @@ +report_comment: > + + This report has been generated by the nf-core/readsimulator + analysis pipeline. 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charset=utf-8 + +$email_html + +--nfcoremimeboundary +Content-Type: image/png;name="nf-core-readsimulator_logo.png" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: inline; filename="nf-core-readsimulator_logo_light.png" + +<% out << new File("$projectDir/assets/nf-core-readsimulator_logo_light.png"). + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' ) %> + +<% +if (mqcFile){ +def mqcFileObj = new File("$mqcFile") +if (mqcFileObj.length() < mqcMaxSize){ +out << """ +--nfcoremimeboundary +Content-Type: text/html; name=\"multiqc_report\" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" + +${mqcFileObj. + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} +""" +}} +%> + +--nfcoremimeboundary-- diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/slackreport.json b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/slackreport.json new file mode 100644 index 0000000..e9d5f8a --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/assets/slackreport.json @@ -0,0 +1,34 @@ +{ + "attachments": [ + { + "fallback": "Plain-text summary of the attachment.", + "color": "<% if (success) { %>good<% } else { %>danger<%} %>", + "author_name": "nf-core/readsimulator ${version} - ${runName}", + "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", + "fields": [ + { + "title": "Command used to launch the workflow", + "value": "```${commandLine}```", + "short": false + } + <% + if (!success) { %> + , + { + "title": "Full error message", + "value": "```${errorReport}```", + "short": false + }, + { + "title": "Pipeline configuration", + "value": "<% out << summary.collect{ k,v -> k == "hook_url" ? "_${k}_: (_hidden_)" : ( ( v.class.toString().contains('Path') || ( v.class.toString().contains('String') && v.contains('/') ) ) ? "_${k}_: `${v}`" : (v.class.toString().contains('DateTime') ? ("_${k}_: " + v.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM))) : "_${k}_: ${v}") ) }.join(",\n") %>", + "short": false + } + <% } + %> + ], + "footer": "Completed at <% out << dateComplete.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM)) %> (duration: ${duration})" + } + ] +} diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/bin/check_samplesheet.py b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/bin/check_samplesheet.py new file mode 100755 index 0000000..4a758fe --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/bin/check_samplesheet.py @@ -0,0 +1,259 @@ +#!/usr/bin/env python + + +"""Provide a command line tool to validate and transform tabular samplesheets.""" + + +import argparse +import csv +import logging +import sys +from collections import Counter +from pathlib import Path + +logger = logging.getLogger() + + +class RowChecker: + """ + Define a service that can validate and transform each given row. + + Attributes: + modified (list): A list of dicts, where each dict corresponds to a previously + validated and transformed row. The order of rows is maintained. + + """ + + VALID_FORMATS = ( + ".fq.gz", + ".fastq.gz", + ) + + def __init__( + self, + sample_col="sample", + first_col="fastq_1", + second_col="fastq_2", + single_col="single_end", + **kwargs, + ): + """ + Initialize the row checker with the expected column names. + + Args: + sample_col (str): The name of the column that contains the sample name + (default "sample"). + first_col (str): The name of the column that contains the first (or only) + FASTQ file path (default "fastq_1"). + second_col (str): The name of the column that contains the second (if any) + FASTQ file path (default "fastq_2"). + single_col (str): The name of the new column that will be inserted and + records whether the sample contains single- or paired-end sequencing + reads (default "single_end"). + + """ + super().__init__(**kwargs) + self._sample_col = sample_col + self._first_col = first_col + self._second_col = second_col + self._single_col = single_col + self._seen = set() + self.modified = [] + + def validate_and_transform(self, row): + """ + Perform all validations on the given row and insert the read pairing status. + + Args: + row (dict): A mapping from column headers (keys) to elements of that row + (values). + + """ + self._validate_sample(row) + self._validate_first(row) + self._validate_second(row) + self._validate_pair(row) + self._seen.add((row[self._sample_col], row[self._first_col])) + self.modified.append(row) + + def _validate_sample(self, row): + """Assert that the sample name exists and convert spaces to underscores.""" + if len(row[self._sample_col]) <= 0: + raise AssertionError("Sample input is required.") + # Sanitize samples slightly. + row[self._sample_col] = row[self._sample_col].replace(" ", "_") + + def _validate_first(self, row): + """Assert that the first FASTQ entry is non-empty and has the right format.""" + if len(row[self._first_col]) <= 0: + raise AssertionError("At least the first FASTQ file is required.") + self._validate_fastq_format(row[self._first_col]) + + def _validate_second(self, row): + """Assert that the second FASTQ entry has the right format if it exists.""" + if len(row[self._second_col]) > 0: + self._validate_fastq_format(row[self._second_col]) + + def _validate_pair(self, row): + """Assert that read pairs have the same file extension. Report pair status.""" + if row[self._first_col] and row[self._second_col]: + row[self._single_col] = False + first_col_suffix = Path(row[self._first_col]).suffixes[-2:] + second_col_suffix = Path(row[self._second_col]).suffixes[-2:] + if first_col_suffix != second_col_suffix: + raise AssertionError("FASTQ pairs must have the same file extensions.") + else: + row[self._single_col] = True + + def _validate_fastq_format(self, filename): + """Assert that a given filename has one of the expected FASTQ extensions.""" + if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): + raise AssertionError( + f"The FASTQ file has an unrecognized extension: {filename}\n" + f"It should be one of: {', '.join(self.VALID_FORMATS)}" + ) + + def validate_unique_samples(self): + """ + Assert that the combination of sample name and FASTQ filename is unique. + + In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the + number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. + + """ + if len(self._seen) != len(self.modified): + raise AssertionError("The pair of sample name and FASTQ must be unique.") + seen = Counter() + for row in self.modified: + sample = row[self._sample_col] + seen[sample] += 1 + row[self._sample_col] = f"{sample}_T{seen[sample]}" + + +def read_head(handle, num_lines=10): + """Read the specified number of lines from the current position in the file.""" + lines = [] + for idx, line in enumerate(handle): + if idx == num_lines: + break + lines.append(line) + return "".join(lines) + + +def sniff_format(handle): + """ + Detect the tabular format. + + Args: + handle (text file): A handle to a `text file`_ object. The read position is + expected to be at the beginning (index 0). + + Returns: + csv.Dialect: The detected tabular format. + + .. _text file: + https://docs.python.org/3/glossary.html#term-text-file + + """ + peek = read_head(handle) + handle.seek(0) + sniffer = csv.Sniffer() + dialect = sniffer.sniff(peek) + return dialect + + +def check_samplesheet(file_in, file_out): + """ + Check that the tabular samplesheet has the structure expected by nf-core pipelines. + + Validate the general shape of the table, expected columns, and each row. Also add + an additional column which records whether one or two FASTQ reads were found. + + Args: + file_in (pathlib.Path): The given tabular samplesheet. The format can be either + CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. + file_out (pathlib.Path): Where the validated and transformed samplesheet should + be created; always in CSV format. + + Example: + This function checks that the samplesheet follows the following structure, + see also the `viral recon samplesheet`_:: + + sample,fastq_1,fastq_2 + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + + .. _viral recon samplesheet: + https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + + """ + required_columns = {"sample", "fastq_1", "fastq_2"} + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_in.open(newline="") as in_handle: + reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) + # Validate the existence of the expected header columns. + if not required_columns.issubset(reader.fieldnames): + req_cols = ", ".join(required_columns) + logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") + sys.exit(1) + # Validate each row. + checker = RowChecker() + for i, row in enumerate(reader): + try: + checker.validate_and_transform(row) + except AssertionError as error: + logger.critical(f"{str(error)} On line {i + 2}.") + sys.exit(1) + checker.validate_unique_samples() + header = list(reader.fieldnames) + header.insert(1, "single_end") + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_out.open(mode="w", newline="") as out_handle: + writer = csv.DictWriter(out_handle, header, delimiter=",") + writer.writeheader() + for row in checker.modified: + writer.writerow(row) + + +def parse_args(argv=None): + """Define and immediately parse command line arguments.""" + parser = argparse.ArgumentParser( + description="Validate and transform a tabular samplesheet.", + epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", + ) + parser.add_argument( + "file_in", + metavar="FILE_IN", + type=Path, + help="Tabular input samplesheet in CSV or TSV format.", + ) + parser.add_argument( + "file_out", + metavar="FILE_OUT", + type=Path, + help="Transformed output samplesheet in CSV format.", + ) + parser.add_argument( + "-l", + "--log-level", + help="The desired log level (default WARNING).", + choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), + default="WARNING", + ) + return parser.parse_args(argv) + + +def main(argv=None): + """Coordinate argument parsing and program execution.""" + args = parse_args(argv) + logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") + if not args.file_in.is_file(): + logger.error(f"The given input file {args.file_in} was not found!") + sys.exit(2) + args.file_out.parent.mkdir(parents=True, exist_ok=True) + check_samplesheet(args.file_in, args.file_out) + + +if __name__ == "__main__": + sys.exit(main()) diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/NfcoreTemplate.groovy b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/NfcoreTemplate.groovy new file mode 100755 index 0000000..e248e4c --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/NfcoreTemplate.groovy @@ -0,0 +1,356 @@ +// +// This file holds several functions used within the nf-core pipeline template. +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +class NfcoreTemplate { + + // + // Check AWS Batch related parameters have been specified correctly + // + public static void awsBatch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + // Check params.awsqueue and params.awsregion have been set if running on AWSBatch + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + } + } + + // + // Warn if a -profile or Nextflow config has not been provided to run the pipeline + // + public static void checkConfigProvided(workflow, log) { + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + } + } + + // + // Generate version string + // + public static String version(workflow) { + String version_string = "" + + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string + } + + // + // Construct and send completion email + // + public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = NfcoreTemplate.version(workflow) + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() + } + + // + // Construct and send a notification to a web server as JSON + // e.g. Microsoft Teams and Slack + // + public static void IM_notification(workflow, params, summary_params, projectDir, log) { + def hook_url = params.hook_url + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = NfcoreTemplate.version(workflow) + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("$projectDir/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } + } + + // + // Dump pipeline parameters in a json file + // + public static void dump_parameters(workflow, params) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() + } + + // + // Print pipeline summary on completion + // + public static void summary(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } + + // + // ANSII Colours used for terminal logging + // + public static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + // + // Does what is says on the tin + // + public static String dashedLine(monochrome_logs) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + // + // nf-core logo + // + public static String logo(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String workflow_version = NfcoreTemplate.version(workflow) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/NftestUtils.groovy b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/NftestUtils.groovy new file mode 100644 index 0000000..ce6fc8b --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/NftestUtils.groovy @@ -0,0 +1,38 @@ +// Helper functions for pipeline tests + +class NftestUtils { + + // Function to remove Nextflow version from software_versions.yml + public static String removeNextflowVersion(outputDir) { + def softwareVersions = path("$outputDir/pipeline_info/software_versions.yml").yaml + if (softwareVersions.containsKey("Workflow")) { + softwareVersions.Workflow.remove("Nextflow") + } + return softwareVersions + } + + // Function to filter lines from a file and return a new file + public static File filterLines(String inFilePath, int linesToSkip) { + if (linesToSkip >= 0) { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lineCount = 0 + inputFile.eachLine { line -> + lineCount++ + if (lineCount > linesToSkip) { + outputFile.append(line + '\n') + } + } + return outputFile + } else { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lines = inputFile.readLines() + def totalLines = lines.size() + lines.take(totalLines + linesToSkip).each { line -> + outputFile.append(line + '\n') + } + return outputFile + } + } +} diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/Utils.groovy b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/Utils.groovy new file mode 100644 index 0000000..8d030f4 --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/Utils.groovy @@ -0,0 +1,47 @@ +// +// This file holds several Groovy functions that could be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml + +class Utils { + + // + // When running with -profile conda, warn if channels have not been set-up appropriately + // + public static void checkCondaChannels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } + } +} diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/WorkflowMain.groovy b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/WorkflowMain.groovy new file mode 100755 index 0000000..477ddaf --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/WorkflowMain.groovy @@ -0,0 +1,77 @@ +// +// This file holds several functions specific to the main.nf workflow in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow + +class WorkflowMain { + + // + // Citation string for pipeline + // + public static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + // TODO nf-core: Add Zenodo DOI for pipeline after first release + //"* The pipeline\n" + + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + + // + // Validate parameters and print summary to screen + // + public static void initialise(workflow, params, log, args) { + + // Print workflow version and exit on --version + if (params.version) { + String workflow_version = NfcoreTemplate.version(workflow) + log.info "${workflow.manifest.name} ${workflow_version}" + System.exit(0) + } + + // Check that a -profile or Nextflow config has been provided to run the pipeline + NfcoreTemplate.checkConfigProvided(workflow, log) + // Check that the profile doesn't contain spaces and doesn't end with a trailing comma + checkProfile(workflow.profile, args, log) + + // Check that conda channels are set-up correctly + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + Utils.checkCondaChannels(log) + } + + // Check AWS batch settings + NfcoreTemplate.awsBatch(workflow, params) + + // Check input has been provided + if (!params.input) { + Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") + } + } + // + // Get attribute from genome config file e.g. fasta + // + public static Object getGenomeAttribute(params, attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null + } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } +} diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/WorkflowReadsimulator.groovy b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/WorkflowReadsimulator.groovy new file mode 100755 index 0000000..c0b9df0 --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib/WorkflowReadsimulator.groovy @@ -0,0 +1,131 @@ +// +// This file holds several functions specific to the workflow/readsimulator.nf in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow +import groovy.text.SimpleTemplateEngine + +class WorkflowReadsimulator { + + // + // Check and validate parameters + // + public static void initialise(params, log) { + + genomeExistsError(params, log) + + if (!params.fasta && !params.ncbidownload_accessions && !params.ncbidownload_taxids) { + Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file. Alternatively, '--ncbidownload_accessions accessions.txt' or '--ncbidownload_taxids taxids.txt' can be used." + } + } + + // + // Get workflow summary for MultiQC + // + public static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } + + // + // Generate methods description for MultiQC + // + + public static String toolCitationText(params) { + def citation_text = [ + "Tools used in the workflow included:", + params["amplicon"] ? [ + "CRABS (Jeunen et al. 2022),", + "ART (Huang et al. 2012)," + ].join(' ').trim() : "", + params["target_capture"] ? [ + "Bowtie2 (Langmead et al. 2012),", + "Samtools (Danecek et al. 2021),", + "CapSim (Cao et al. 2018)," + ].join(' ').trim() : "", + params["metagenome"] ? + "InSilicoSeq (Gourlé et al. 2018)," : "", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text + } + + public static String toolBibliographyText(params) { + def reference_text = [ + params["amplicon"] ? [ + "
  • Jeunen, G.-J., Dowle, E., Edgecombe, J., von Ammon, U., Gemmell, N. J., & Cross, H. (2022). crabs—A software program to generate curated reference databases for metabarcoding sequencing data. Molecular Ecology Resources, 00, 1– 14. https://doi.org/10.1111/1755-0998.13741
    • ", + "
  • Weichun Huang, Leping Li, Jason R. Myers, Gabor T. Marth, ART: a next-generation sequencing read simulator, Bioinformatics, Volume 28, Issue 4, February 2012, Pages 593–594, https://doi.org/10.1093/bioinformatics/btr708
    • " + ].join(' ').trim() : "", + params["target_capture"] ? [ + "
  • Langmead, B., Salzberg, S. Fast gapped-read alignment with Bowtie 2. Nat Methods 9, 357–359 (2012). https://doi.org/10.1038/nmeth.1923
    • ", + "
  • Twelve years of SAMtools and BCFtools. Petr Danecek, James K Bonfield, Jennifer Liddle, John Marshall, Valeriu Ohan, Martin O Pollard, Andrew Whitwham, Thomas Keane, Shane A McCarthy, Robert M Davies, Heng Li. GigaScience, Volume 10, Issue 2, February 2021, giab008, https://doi.org/10.1093/gigascience/giab008
    • ", + "
  • Minh Duc Cao, Devika Ganesamoorthy, Chenxi Zhou, Lachlan J M Coin, Simulating the dynamics of targeted capture sequencing with CapSim, Bioinformatics, Volume 34, Issue 5, March 2018, Pages 873–874, https://doi.org/10.1093/bioinformatics/btx691
    • " + ].join(' ').trim() : "", + params["metagenome"] ? + "
  • Gourlé H, Karlsson-Lindsjö O, Hayer J and Bongcam+Rudloff E, Simulating Illumina data with InSilicoSeq. Bioinformatics (2018) doi:10.1093/bioinformatics/bty630
    • " : "", + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text + } + + public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = run_workflow.toMap() + meta["manifest_map"] = run_workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + meta["tool_bibliography"] = toolBibliographyText(params) + + + def methods_text = mqc_methods_yaml.text + + def engine = new SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html + } + + // + // Exit pipeline if incorrect --genome key provided + // + private static void genomeExistsError(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + Nextflow.error(error_string) + } + } +} diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/mock.nf b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/mock.nf new file mode 100644 index 0000000..19bf900 --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/mock.nf @@ -0,0 +1,88 @@ +import groovy.json.JsonGenerator +import groovy.json.JsonGenerator.Converter + +nextflow.enable.dsl=2 + +// comes from nf-test to store json files +params.nf_test_output = "" + +// include dependencies + + +// include test process +include { FASTQC } from '/workspaces/readsimulator/./modules/nf-core/fastqc/tests/../main.nf' + +// define custom rules for JSON that will be generated. +def jsonOutput = + new JsonGenerator.Options() + .excludeNulls() // Do not include fields with value null.. + .addConverter(Path) { value -> value.toString() } // Custom converter for Path. Only filename + .build() + + +workflow { + + // run dependencies + + + // process mapping + def input = [] + + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + + //---- + + //run process + FASTQC(*input) + + if (FASTQC.output){ + + // consumes all named output channels and stores items in a json file + for (def name in FASTQC.out.getNames()) { + serializeChannel(name, FASTQC.out.getProperty(name), jsonOutput) + } + + // consumes all unnamed output channels and stores items in a json file + def array = FASTQC.out as Object[] + for (def i = 0; i < array.length ; i++) { + serializeChannel(i, array[i], jsonOutput) + } + + } + +} + +def serializeChannel(name, channel, jsonOutput) { + def _name = name + def list = [ ] + channel.subscribe( + onNext: { + list.add(it) + }, + onComplete: { + def map = new HashMap() + map[_name] = list + def filename = "${params.nf_test_output}/output_${_name}.json" + new File(filename).text = jsonOutput.toJson(map) + } + ) +} + + +workflow.onComplete { + + def result = [ + success: workflow.success, + exitStatus: workflow.exitStatus, + errorMessage: workflow.errorMessage, + errorReport: workflow.errorReport + ] + new File("${params.nf_test_output}/workflow.json").text = jsonOutput.toJson(result) + +} diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/nextflow.log b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/nextflow.log new file mode 100644 index 0000000..b5df051 --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/nextflow.log @@ -0,0 +1,106 @@ +Feb-02 16:20:21.316 [main] DEBUG nextflow.cli.Launcher - $> nextflow -quiet -log /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/nextflow.log run /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/mock.nf -c /workspaces/readsimulator/nextflow.config -c /workspaces/readsimulator/tests/nextflow.config -params-file /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/params.json -ansi-log false -profile singularity -with-trace /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/trace.csv -w /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work +Feb-02 16:20:21.419 [main] INFO nextflow.cli.CmdRun - N E X T F L O W ~ version 23.10.1 +Feb-02 16:20:21.448 [main] DEBUG nextflow.plugin.PluginsFacade - Setting up plugin manager > mode=prod; embedded=false; plugins-dir=/home/gitpod/.nextflow/plugins; core-plugins: nf-amazon@2.1.4,nf-azure@1.3.3,nf-cloudcache@0.3.0,nf-codecommit@0.1.5,nf-console@1.0.6,nf-ga4gh@1.1.0,nf-google@1.8.3,nf-tower@1.6.3,nf-wave@1.0.1 +Feb-02 16:20:21.467 [main] INFO o.pf4j.DefaultPluginStatusProvider - Enabled plugins: [] +Feb-02 16:20:21.469 [main] INFO o.pf4j.DefaultPluginStatusProvider - Disabled plugins: [] +Feb-02 16:20:21.475 [main] INFO org.pf4j.DefaultPluginManager - PF4J version 3.4.1 in 'deployment' mode +Feb-02 16:20:21.491 [main] INFO org.pf4j.AbstractPluginManager - No plugins +Feb-02 16:20:21.512 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /workspaces/readsimulator/nextflow.config +Feb-02 16:20:21.513 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /workspaces/readsimulator/tests/nextflow.config +Feb-02 16:20:21.514 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /workspaces/readsimulator/nextflow.config +Feb-02 16:20:21.514 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /workspaces/readsimulator/tests/nextflow.config +Feb-02 16:20:21.552 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity` +Feb-02 16:20:24.461 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity` +Feb-02 16:20:25.341 [main] DEBUG nextflow.config.ConfigBuilder - Available config profiles: [cfc_dev, uzl_omics, ifb_core, denbi_qbic, alice, mjolnir_globe, uppmax, incliva, ilifu, uge, rosalind_uge, lugh, unibe_ibu, vai, czbiohub_aws, jax, ccga_med, scw, tigem, tubingen_apg, google, apollo, ipop_up, pdc_kth, googlels, daisybio, eddie, medair, biowulf, apptainer, bi, bigpurple, adcra, cedars, pawsey_setonix, vsc_kul_uhasselt, pawsey_nimbus, ucl_myriad, utd_ganymede, charliecloud, icr_davros, ceres, munin, arm, rosalind, hasta, cfc, uzh, ebi_codon_slurm, ebc, ku_sund_dangpu, ccga_dx, crick, marvin, biohpc_gen, shifter, mana, mamba, unc_lccc, wehi, awsbatch, wustl_htcf, imperial, maestro, software_license, genotoul, nci_gadi, abims, janelia, nu_genomics, googlebatch, oist, sahmri, mpcdf, leicester, vsc_ugent, create, sage, cambridge, jex, podman, ebi_codon, cheaha, xanadu, nyu_hpc, test, computerome, ucd_sonic, seg_globe, sanger, dkfz, pasteur, ethz_euler, test_full, imb, tuos_stanage, azurebatch, hki, crukmi, csiro_petrichor, docker, engaging, gis, hypatia, psmn, eva, nygc, fgcz, conda, crg, singularity, self_hosted_runner, uw_hyak_pedslabs, utd_sysbio, debug, genouest, cbe, phoenix, gitpod, seawulf, uod_hpc, fub_curta, uct_hpc, aws_tower, binac] +Feb-02 16:20:25.421 [main] DEBUG nextflow.cli.CmdRun - Applied DSL=2 from script declararion +Feb-02 16:20:25.445 [main] INFO nextflow.cli.CmdRun - Launching `/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/mock.nf` [focused_snyder] DSL2 - revision: f7ddc0004f +Feb-02 16:20:25.445 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins declared=[nf-validation@1.1.3] +Feb-02 16:20:25.446 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins default=[] +Feb-02 16:20:25.446 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins resolved requirement=[nf-validation@1.1.3] +Feb-02 16:20:25.446 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-validation version: 1.1.3 +Feb-02 16:20:25.451 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-validation@1.1.3' resolved +Feb-02 16:20:25.452 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-validation@1.1.3' +Feb-02 16:20:25.459 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-validation@1.1.3 +Feb-02 16:20:25.467 [main] DEBUG n.secret.LocalSecretsProvider - Secrets store: /home/gitpod/.nextflow/secrets/store.json +Feb-02 16:20:25.471 [main] DEBUG nextflow.secret.SecretsLoader - Discovered secrets providers: [nextflow.secret.LocalSecretsProvider@2ee92e7d] - activable => nextflow.secret.LocalSecretsProvider@2ee92e7d +Feb-02 16:20:25.585 [main] DEBUG nextflow.Session - Session UUID: 97870fad-fa17-41c6-9dc4-94d8f386cff1 +Feb-02 16:20:25.585 [main] DEBUG nextflow.Session - Run name: focused_snyder +Feb-02 16:20:25.586 [main] DEBUG nextflow.Session - Executor pool size: 8 +Feb-02 16:20:25.599 [main] DEBUG nextflow.file.FilePorter - File porter settings maxRetries=3; maxTransfers=50; pollTimeout=null +Feb-02 16:20:25.604 [main] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'FileTransfer' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:20:25.635 [main] DEBUG nextflow.cli.CmdRun - + Version: 23.10.1 build 5891 + Created: 12-01-2024 22:01 UTC (22:01 GMT) + System: Linux 5.15.133.1-microsoft-standard-WSL2 + Runtime: Groovy 3.0.19 on OpenJDK 64-Bit Server VM 17.0.10-internal+0-adhoc..src + Encoding: UTF-8 (UTF-8) + Process: 18259@b23599a75663 [172.17.0.3] + CPUs: 8 - Mem: 7.6 GB (415.1 MB) - Swap: 2 GB (2 GB) +Feb-02 16:20:25.678 [main] DEBUG nextflow.Session - Work-dir: /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work [ext2/ext3] +Feb-02 16:20:25.699 [main] DEBUG nextflow.executor.ExecutorFactory - Extension executors providers=[] +Feb-02 16:20:25.717 [main] DEBUG nextflow.Session - Observer factory: DefaultObserverFactory +Feb-02 16:20:25.772 [main] DEBUG nextflow.cache.CacheFactory - Using Nextflow cache factory: nextflow.cache.DefaultCacheFactory +Feb-02 16:20:25.793 [main] DEBUG nextflow.util.CustomThreadPool - Creating default thread pool > poolSize: 9; maxThreads: 1000 +Feb-02 16:20:25.891 [main] DEBUG nextflow.Session - Session start +Feb-02 16:20:25.897 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow started -- trace file: /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/trace.csv +Feb-02 16:20:25.904 [main] DEBUG nextflow.Session - Using default localLib path: /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib +Feb-02 16:20:25.910 [main] DEBUG nextflow.Session - Adding to the classpath library: /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/lib +Feb-02 16:20:26.253 [main] DEBUG nextflow.script.ScriptRunner - > Launching execution +Feb-02 16:20:26.517 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-amazon version: 2.1.4 +Feb-02 16:20:26.523 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-amazon@2.1.4' resolved +Feb-02 16:20:26.523 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-amazon@2.1.4' +Feb-02 16:20:26.536 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-amazon@2.1.4 +Feb-02 16:20:26.551 [main] DEBUG nextflow.file.FileHelper - > Added 'S3FileSystemProvider' to list of installed providers [s3] +Feb-02 16:20:26.551 [main] DEBUG nextflow.file.FileHelper - Started plugin 'nf-amazon' required to handle file: s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_1.fastq.gz +Feb-02 16:20:26.561 [main] DEBUG nextflow.file.FileHelper - Creating a file system instance for provider: S3FileSystemProvider +Feb-02 16:20:26.574 [main] DEBUG nextflow.cloud.aws.config.AwsConfig - AWS S3 config properties: {max_error_retry=5} +Feb-02 16:20:27.115 [main] DEBUG nextflow.cloud.aws.nio.S3Client - Setting S3 glacierRetrievalTier=null +Feb-02 16:20:27.216 [main] DEBUG n.c.aws.util.S3CredentialsProvider - No AWS credentials available - falling back to anonymous access +Feb-02 16:20:29.785 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name FASTQC +Feb-02 16:20:29.787 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:FASTQC` matches process FASTQC +Feb-02 16:20:29.788 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process FASTQC +Feb-02 16:20:29.796 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Feb-02 16:20:29.796 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Feb-02 16:20:29.804 [main] DEBUG nextflow.executor.Executor - [warm up] executor > local +Feb-02 16:20:29.813 [main] DEBUG n.processor.LocalPollingMonitor - Creating local task monitor for executor 'local' > cpus=8; memory=7.6 GB; capacity=8; pollInterval=100ms; dumpInterval=5m +Feb-02 16:20:29.816 [main] DEBUG n.processor.TaskPollingMonitor - >>> barrier register (monitor: local) +Feb-02 16:20:29.918 [main] DEBUG nextflow.Session - Config process names validation disabled as requested +Feb-02 16:20:29.918 [main] DEBUG nextflow.Session - Igniting dataflow network (2) +Feb-02 16:20:29.918 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > FASTQC +Feb-02 16:20:29.919 [main] DEBUG nextflow.script.ScriptRunner - Parsed script files: + Script_f1825f5cbbf97878: /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/mock.nf + Script_4ba798786ecd6418: /workspaces/readsimulator/./modules/nf-core/fastqc/tests/../main.nf +Feb-02 16:20:29.919 [main] DEBUG nextflow.script.ScriptRunner - > Awaiting termination +Feb-02 16:20:29.919 [main] DEBUG nextflow.Session - Session await +Feb-02 16:20:30.781 [FileTransfer-1] DEBUG nextflow.file.FilePorter - Copying foreign file s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_1.fastq.gz to work dir: /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/stage-97870fad-fa17-41c6-9dc4-94d8f386cff1/f5/0c82303ab09f74f6a97d4324db8586/test_1.fastq.gz +Feb-02 16:20:30.781 [FileTransfer-2] DEBUG nextflow.file.FilePorter - Copying foreign file s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_2.fastq.gz to work dir: /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/stage-97870fad-fa17-41c6-9dc4-94d8f386cff1/ec/8f69d2605fb45d3589f540df65dffd/test_2.fastq.gz +Feb-02 16:20:30.782 [FileTransfer-3] DEBUG nextflow.file.FilePorter - Copying foreign file s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test2_1.fastq.gz to work dir: /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/stage-97870fad-fa17-41c6-9dc4-94d8f386cff1/27/1317deb2f7b742137d7ca25cf17f83/test2_1.fastq.gz +Feb-02 16:20:30.783 [FileTransfer-4] DEBUG nextflow.file.FilePorter - Copying foreign file s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test2_2.fastq.gz to work dir: /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/stage-97870fad-fa17-41c6-9dc4-94d8f386cff1/3a/66ac27c35b59f65a0a7c15f0785da1/test2_2.fastq.gz +Feb-02 16:20:30.982 [FileTransfer-1] DEBUG n.cloud.aws.nio.S3FileSystemProvider - S3 download file from=s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_1.fastq.gz to=/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/stage-97870fad-fa17-41c6-9dc4-94d8f386cff1/f5/0c82303ab09f74f6a97d4324db8586/test_1.fastq.gz +Feb-02 16:20:30.982 [FileTransfer-1] DEBUG nextflow.cloud.aws.nio.S3Client - Creating S3 transfer manager pool - chunk-size=104857600; max-treads=10; +Feb-02 16:20:30.988 [FileTransfer-1] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'S3TransferManager' minSize=10; maxSize=10; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:20:31.656 [FileTransfer-4] DEBUG n.cloud.aws.nio.S3FileSystemProvider - S3 download file from=s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test2_2.fastq.gz to=/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/stage-97870fad-fa17-41c6-9dc4-94d8f386cff1/3a/66ac27c35b59f65a0a7c15f0785da1/test2_2.fastq.gz +Feb-02 16:20:31.661 [FileTransfer-3] DEBUG n.cloud.aws.nio.S3FileSystemProvider - S3 download file from=s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test2_1.fastq.gz to=/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/stage-97870fad-fa17-41c6-9dc4-94d8f386cff1/27/1317deb2f7b742137d7ca25cf17f83/test2_1.fastq.gz +Feb-02 16:20:31.665 [FileTransfer-2] DEBUG n.cloud.aws.nio.S3FileSystemProvider - S3 download file from=s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_2.fastq.gz to=/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/stage-97870fad-fa17-41c6-9dc4-94d8f386cff1/ec/8f69d2605fb45d3589f540df65dffd/test_2.fastq.gz +Feb-02 16:20:32.330 [Actor Thread 7] WARN nextflow.container.SingularityCache - Singularity cache directory has not been defined -- Remote image will be stored in the path: /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/singularity -- Use the environment variable NXF_SINGULARITY_CACHEDIR to specify a different location +Feb-02 16:20:32.330 [Actor Thread 7] INFO nextflow.container.SingularityCache - Pulling Singularity image https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0 [cache /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/singularity/depot.galaxyproject.org-singularity-fastqc-0.12.1--hdfd78af_0.img] +Feb-02 16:20:33.520 [Actor Thread 7] DEBUG nextflow.container.SingularityCache - Singularity pull complete image=https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0 path=/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/singularity/depot.galaxyproject.org-singularity-fastqc-0.12.1--hdfd78af_0.img +Feb-02 16:20:33.667 [Task submitter] DEBUG n.executor.local.LocalTaskHandler - Launch cmd line: /bin/bash -ue .command.run +Feb-02 16:20:33.670 [Task submitter] INFO nextflow.Session - [3a/cca3cf] Submitted process > FASTQC (test) +Feb-02 16:20:42.260 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[id: 1; name: FASTQC (test); status: COMPLETED; exit: 0; error: -; workDir: /workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/3a/cca3cf1a6c5458c19bedefc40607c1] +Feb-02 16:20:42.314 [Task monitor] WARN nextflow.processor.PublishDir - Process `FASTQC` publishDir path contains a variable with a null value +Feb-02 16:20:42.322 [Task monitor] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'PublishDir' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:20:42.336 [main] DEBUG nextflow.Session - Session await > all processes finished +Feb-02 16:20:42.341 [Task monitor] DEBUG n.processor.TaskPollingMonitor - <<< barrier arrives (monitor: local) - terminating tasks monitor poll loop +Feb-02 16:20:42.341 [main] DEBUG nextflow.Session - Session await > all barriers passed +Feb-02 16:20:42.346 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'PublishDir' shutdown completed (hard=false) +Feb-02 16:20:42.363 [main] DEBUG n.trace.WorkflowStatsObserver - Workflow completed > WorkflowStats[succeededCount=1; failedCount=0; ignoredCount=0; cachedCount=0; pendingCount=0; submittedCount=0; runningCount=0; retriesCount=0; abortedCount=0; succeedDuration=16s; failedDuration=0ms; cachedDuration=0ms;loadCpus=0; loadMemory=0; peakRunning=1; peakCpus=2; peakMemory=3 GB; ] +Feb-02 16:20:42.364 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow completed -- saving trace file +Feb-02 16:20:42.460 [main] DEBUG nextflow.cache.CacheDB - Closing CacheDB done +Feb-02 16:20:42.461 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-amazon@2.1.4' +Feb-02 16:20:42.461 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-amazon +Feb-02 16:20:42.461 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-validation@1.1.3' +Feb-02 16:20:42.461 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-validation +Feb-02 16:20:42.480 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'FileTransfer' shutdown completed (hard=false) +Feb-02 16:20:42.481 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'S3TransferManager' shutdown completed (hard=false) +Feb-02 16:20:42.481 [main] DEBUG nextflow.script.ScriptRunner - > Execution complete -- Goodbye diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/output_0.json b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/output_0.json new file mode 100644 index 0000000..a9198be --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/output_0.json @@ -0,0 +1 @@ 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+{"2":["/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/3a/cca3cf1a6c5458c19bedefc40607c1/versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/output_html.json b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/output_html.json new file mode 100644 index 0000000..396a98c --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/output_html.json @@ -0,0 +1 @@ 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+{"versions":["/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/3a/cca3cf1a6c5458c19bedefc40607c1/versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/output_zip.json b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/output_zip.json new file mode 100644 index 0000000..dd59aeb --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/output_zip.json @@ -0,0 +1 @@ +{"zip":[[{"id":"test","single_end":false},["/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/3a/cca3cf1a6c5458c19bedefc40607c1/test_1_fastqc.zip","/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/3a/cca3cf1a6c5458c19bedefc40607c1/test_2_fastqc.zip","/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/3a/cca3cf1a6c5458c19bedefc40607c1/test_3_fastqc.zip","/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/work/3a/cca3cf1a6c5458c19bedefc40607c1/test_4_fastqc.zip"]]]} \ No newline at end of file diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/params.json b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/params.json new file mode 100644 index 0000000..7e1b9f3 --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/params.json @@ -0,0 +1 @@ +{"nf_test_output":"/workspaces/readsimulator/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta"} \ No newline at end of file diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/std.err b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/std.err new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/std.out b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/std.out new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/trace.csv b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/trace.csv new file mode 100644 index 0000000..ae8a4cf --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/trace.csv @@ -0,0 +1,2 @@ +task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar +1 3a/cca3cf 18448 FASTQC (test) COMPLETED 0 2024-02-02 16:20:33.670 8.6s 8s 161.1% 542.2 MB 3.9 GB 14.1 MB 7.8 MB diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/workflow.json b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/workflow.json new file mode 100644 index 0000000..7d54df0 --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/meta/workflow.json @@ -0,0 +1 @@ +{"success":true,"exitStatus":0} \ No newline at end of file diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/null/fastqc/test_1_fastqc.html b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/null/fastqc/test_1_fastqc.html new file mode 100644 index 0000000..e72abac --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/null/fastqc/test_1_fastqc.html @@ -0,0 +1,187 @@ +test_1.gz FastQC Report
    FastQCFastQC Report
    Fri 2 Feb 2024
    test_1.gz

    Summary

    [OK]Basic Statistics

    MeasureValue
    Filenametest_1.gz
    File typeConventional base calls
    EncodingSanger / Illumina 1.9
    Total Sequences100
    Total Bases13.8 kbp
    Sequences flagged as poor quality0
    Sequence length72-151
    %GC39

    [OK]Per base sequence quality

    Per base quality graph

    [FAIL]Per tile sequence quality

    Per tile quality graph

    [OK]Per sequence quality scores

    Per Sequence quality graph

    [FAIL]Per base sequence content

    Per base sequence content

    [FAIL]Per sequence GC content

    Per sequence GC content graph

    [OK]Per base N content

    N content graph

    [WARN]Sequence Length Distribution

    Sequence length distribution

    [OK]Sequence Duplication Levels

    Duplication level graph

    [FAIL]Overrepresented sequences

    SequenceCountPercentagePossible Source
    CAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGA22.0No Hit
    ACTCTAGATAATTCATTAGGTTTCTTAATAGTAAGACTAGAATTGTCTAC11.0No Hit
    GTCTAATCATAATTTCTTGGTACAGGCTGGTATTGTTCATCTCAGGGTTA11.0No Hit
    TGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTA11.0No Hit
    TTTCAAGACTTGTAAATTGCAACTTGTCATAAAGGTCTCTATCAGACATT11.0No Hit
    TTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTA11.0No Hit
    ATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTA11.0No Hit
    GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT11.0No Hit
    AAGTGTATCTTGATCCTCATAACTCATTGAATCATAATAAAGTCTAGCCT11.0No Hit
    GTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTC11.0No Hit
    AATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTA11.0No Hit
    CAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAAT11.0No Hit
    GTTCCTAGGTAGTAGAAATACCATCTAGGACTGAGATCTTTCATTTTACA11.0No Hit
    CATCTTATTTCTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGA11.0No Hit
    TATCTGCCCATTTAATAGAAGTTAAACCATTAACTTGTGGGTATTTCCAC11.0No Hit
    CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT11.0No Hit
    ACTGAATTTTCTGCACCAAGTGACATAGTGTAGGCAATGATGGATTGACT11.0No Hit
    AACTAGCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGC11.0No Hit
    AAGTTGGCGTATACGCGTAATATATCTGGGTTTTCTACAAAATCATACCA11.0No Hit
    TTCCAGAAGAGCAAGGTTCTTTTAAAAGTACTGTTGTACCTCTAACACAC11.0No Hit
    TATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAA11.0No Hit
    TTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTAATGGGTAA11.0No Hit
    GGTGTATTCAAGGCTCCCTCAGTTGCAACCCATATGATGCCGTCTTTGTT11.0No Hit
    GTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTA11.0No Hit
    AAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTT11.0No Hit
    ACATAGTGTAGGCAATGATGGATTGACTAGCTACACTACGTGCCCGCCGA11.0No Hit
    GTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAA11.0No Hit
    CAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCT11.0No Hit
    GGATAGACAGATCGAATTCTACCCATAAAGCCATCAAGCTTTTTCTTTTC11.0No Hit
    ACTGTTTTCTTTGTAGAAAACATCCGTAATAGGACCTTTGTATTCTGAGG11.0No Hit
    GATGAAACATCATGCGTGATAACACCCTTATAAAACATTTTAAAGCATTG11.0No Hit
    GTTATAACACTGACACCACCAAAAGAACATGGTGTAATGTCAAGAATCTC11.0No Hit
    GCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGT11.0No Hit
    CCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGAC11.0No Hit
    CAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCT11.0No Hit
    CATTAGCACAAGTTGTAGGTATTTGTACATACTTACCTTTTAAGTCACAA11.0No Hit
    GTTACTCACAATTTTGACTTCACTTTTAGTCTTAGTCCAGAGTACTCAAT11.0No Hit
    TGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTG11.0No Hit
    CTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTG11.0No Hit
    TCTTTGTAAGCTTTAGCAGCATCTACAGCAAAAGCACAGAAAGATAATAC11.0No Hit
    ATGTCAGATTTATGTGACAATTTAAGACATTCAACAACATCTTTAGTTTC11.0No Hit
    GGATTAGTAACACTACAGCTGATGCATACATAACACAGTCTTTTAGCTTA11.0No Hit
    CTGTAATAGTTGTGTCCGTACCAGCTGCTTGTGCTGTTTGCCTGTCAACA11.0No Hit
    GAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTG11.0No Hit
    CTTTTACAAGACTTCAGAGTTTAGAAATTGTGGCTTATAATGTTGTAATT11.0No Hit
    TGGCAATAGAGTTATTAGAGTAAGCAACTGAATTTTCTGCACCAAGTGAC11.0No Hit
    CACAAACTCTAAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCAGAT11.0No Hit
    CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGA11.0No Hit
    AACACCAGCTTCTGATCTTTCACAAGTGCCGTGCCTACAGTACTCAGAAT11.0No Hit
    GATTTTTAAGTGTGTCACTTAACATTTGTACAATCTTTATACGCACTACA11.0No Hit
    AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC11.0No Hit
    AGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTA11.0No Hit
    CATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTT11.0No Hit
    GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG11.0No Hit
    ACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAA11.0No Hit
    CACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATA11.0No Hit
    TGCCAATCCTGTAGCGACTGTATGCAGCAAAACCTGAGTCACCTGCTACA11.0No Hit
    GATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGC11.0No Hit
    GTACGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTA11.0No Hit
    CTATAACATTAATAGGCAATGAACCTTTAGTGTTATTAGCTCTCAGGTTG11.0No Hit
    ATAAACATGTTAGGGTAACCATTAACTTGATAATTCATTTTAAAACCCAT11.0No Hit
    CCAAGCAGGGTTACGTGTAAGGAATTCTCTTACCACGCCTATTTGTGGCC11.0No Hit
    GATTTAGCCACATTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGA11.0No Hit
    GTTGAAAGTAGATCTTCAATAAATGACCTCTTGCTTGGTTTTGATGGATC11.0No Hit
    TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCG11.0No Hit
    TAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCG11.0No Hit
    GCTATAACGCAGCCTGTAAAATCATCTGGTAATTTATAATTATAATCAGC11.0No Hit
    AGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTT11.0No Hit
    TCTAAGCATAGTGAAAAGCATTGTCTGCATAGCACTAGTAACTTTTGCCC11.0No Hit
    GAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGA11.0No Hit
    ATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGAT11.0No Hit
    CCGTCTATGCAATACAAAGTTTCTTTAGAAGTTATATGTTTATAGTGACC11.0No Hit
    GCAATTCATAGCTCTTTTCAGAACGTTCCGTGTACCAAGCAATTTCATGC11.0No Hit
    AGTAAACTGTTACAAACAACAACAGCAAGTTGCAAACAAAGTGAACACCC11.0No Hit
    GTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAG11.0No Hit
    GTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTG11.0No Hit
    CTCCAAAGCCACGTACGAGCACGTCGCGAACCTGTAAAACAGGCAAACTG11.0No Hit
    GTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCA11.0No Hit
    GTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAATTGTCTGT11.0No Hit
    GTACAAAAATAGCCTAAGAAACAATAAACTAGCATTATACACTGAAGTGT11.0No Hit
    GATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACA11.0No Hit
    GTGAAATTAGTATGGACAAATCACCTAATTTAGCATGGCCTCTTATTGTA11.0No Hit
    TCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAG11.0No Hit
    GCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGC11.0No Hit
    TGCGTCTTGGGTCCCCGCTCTCACTCAACATGGCAAGGAAGACCTTAAAT11.0No Hit
    AACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCA11.0No Hit
    CTAAAAGTGAAGTCAAAATTGTGAGTAACAACCAGTGGTGTGTACCCGTG11.0No Hit
    ATGTAAGGCTTTGTTAAGTCAGTGTCAACATGTGACTCTGCAGTTAAAGC11.0No Hit
    CCTTGAATGAGTCTAATTCAGGTTGCAAAGGATCATAAACTGTGTTGTTG11.0No Hit
    GCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGC11.0No Hit
    ATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGG11.0No Hit
    GCTGCTAGAGACCTCGTTTGTGCACAAAAGTTTAACGGCCTTACTGTTTT11.0No Hit
    CCCTTTGTTGTGTTGTAGTAAGCTAACGCATTGTCATCAGTGCAAGCAGT11.0No Hit
    ATCCTTTAGGATTTGGATGATCTATGTGGCAACGGCAGTACAGACAACAC11.0No Hit
    CTACACGAGCACGTGCAGGTATAATTCTACTACATTTATCTATAGGCAAA11.0No Hit
    TGCCTGACCAGTACCAGTGTGTGTACACAACATCTTAACACAATTAGTGA11.0No Hit
    GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT11.0No Hit
    ATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTT11.0No Hit
    TTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATA11.0No Hit

    [OK]Adapter Content

    Adapter graph

    Produced by FastQC (version 0.12.1)
    \ No newline at end of file diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/null/fastqc/test_1_fastqc.zip b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/null/fastqc/test_1_fastqc.zip new file mode 100644 index 0000000000000000000000000000000000000000..ff9ebf78c78b7e3642e047c28ba3a6c2898dcf8a GIT binary patch literal 454620 zcmce-1C(UV)-77L(Pg{3Y<1byW!tuG+f`k*ZQHhO+kW+Z=lpk^A7_kr-@D`H&d8lR zV#XRPRz%JjJ9eau1TY8+00aaCK&&6X3c$a-;J&{*7}`5%(`y^)+B?|l(*gs?NPt89 z1p)dePUhd%M)_~G#q_PM?EklHp#FComb%7<|9eJ={=1RC9r;I_v`xP+Xl$&E0V_b{ z-a=)hL|~yXp}woYiirx!eV_6FN)TY*pZ0EU_unTFdwwy6?|4CYKp2F4Uqjl6s@i|! zm;OqC{N!ma-rdH--al~1o;$H z0xOaCBk6&q95!>}0+x*zN zyUB8&`t=l2T8namR5HmI-YeN1*bUmvWCMl{#tjZewQv*oKCB11Oz7CHwN0R-i1hxLxB13<26U?VLBoJPH{ z^{zu4Vb~-J^qkFmt>t?`B7+wQ=KJPr4dZ6+BM=gT2jd808p{$0hs+B7h+v}^-tY6| zqwWU=)YMS+7)+`YF6a~S2I}>)=vSy?leK}Fz9#cS<#kvtgGlKfg3u_uSq=G&7%i$r*Z`6<*G5Xu?q3%4ua;`m(dHjH7KxXfc1kfUW zgy#VeDB=!RNT1S7&`BU7K122Vp=~1lRj*h9S#-0Y73ywOdbKk(SAr=O-3KlpA!6h1 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zHNLPkF(>8Z4s37o_%6)Auwov%!6oU$8;r%p5>hSSD}XMJQDk5+MRDY)f#wU)6#@JP%B-7!IrV@9dhjG) z#$iQDX+chAaw5pl@j0nwsX4{yZU-40c2@ad9xz;-YtaqPyH32pSX?~S=(_zCb_RyL zLpZjN`PfIj?@iu?#{=+BSWMa7|u;cH#J_ANK>)675Qog1r)a~ zHbgfSTCW$S7UUO|=w+1T<{;NtAU7fpHKNwWnZ0aiK1aLU0o^F%HWg@W5&@py!fq6B Yq%y#p60CnaICjbBd literal 0 HcmV?d00001 diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/null/fastqc/test_2_fastqc.html b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/null/fastqc/test_2_fastqc.html new file mode 100644 index 0000000..cd52525 --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/null/fastqc/test_2_fastqc.html @@ -0,0 +1,187 @@ +test_2.gz FastQC Report
    FastQCFastQC Report
    Fri 2 Feb 2024
    test_2.gz

    Summary

    [OK]Basic Statistics

    MeasureValue
    Filenametest_2.gz
    File typeConventional base calls
    EncodingSanger / Illumina 1.9
    Total Sequences100
    Total Bases13.7 kbp
    Sequences flagged as poor quality0
    Sequence length53-151
    %GC39

    [OK]Per base sequence quality

    Per base quality graph

    [FAIL]Per tile sequence quality

    Per tile quality graph

    [OK]Per sequence quality scores

    Per Sequence quality graph

    [FAIL]Per base sequence content

    Per base sequence content

    [FAIL]Per sequence GC content

    Per sequence GC content graph

    [OK]Per base N content

    N content graph

    [WARN]Sequence Length Distribution

    Sequence length distribution

    [OK]Sequence Duplication Levels

    Duplication level graph

    [FAIL]Overrepresented sequences

    SequenceCountPercentagePossible Source
    CAATAGTCCAGTCAACACGCTTAACAAAGCACTCGTGGACAGCTAGACAC22.0No Hit
    ATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTA11.0No Hit
    CCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCC11.0No Hit
    TTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGC11.0No Hit
    ATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGATGCGAGAGACTTGT11.0No Hit
    CTTAGGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTACACAATTT11.0No Hit
    GTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAAT11.0No Hit
    CCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATA11.0No Hit
    TTGTCATCAGTGCAAGCAGTTTGTGTAGTACCGGCAGCACAAGACATCTG11.0No Hit
    CCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTG11.0No Hit
    GTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTT11.0No Hit
    ATTATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGG11.0No Hit
    GCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACG11.0No Hit
    TCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGAT11.0No Hit
    CATAGTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCATAATTT11.0No Hit
    ACATACTGTTCTAATGTTGAAGTCACTTTGAATTTATCAAAACACTCTAC11.0No Hit
    CTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGT11.0No Hit
    CCCTTGGAGAGTGCTAGTTGCCATCTCTTTTTGAGGGTTATGATTTTGGA11.0No Hit
    GTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGACATGT11.0No Hit
    CCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGT11.0No Hit
    GGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCAT11.0No Hit
    ATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTA11.0No Hit
    TTAGTAGCCGTATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGTA11.0No Hit
    GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG11.0No Hit
    TGATCAACCTATCAATTTGCACTTCAGCCTCAACTTTGTCAAGACGTGAA11.0No Hit
    CTGTGGATCACGGACAGCATCAGTAGTGTCAGCAATGTCTCTGCCAAATT11.0No Hit
    GGTGGCTGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATC11.0No Hit
    CTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG11.0No Hit
    CTCAGTTGCAACCCATATGATGCCGTCTTTGTTAGCACCATAGGGAAGTC11.0No Hit
    GCGTACAACCAAGCTAAAGTTACTGGCCATAACAGCCAGAGGAAAATTAA11.0No Hit
    CCGCTAACAGTGCAGAAGTGTATTGAGCAATCATTTCATCTGTGAGCAAA11.0No Hit
    AGATAATAGATGGTAGAATGTAAAAGGCACTTTTACACTTTTTAAGCACT11.0No Hit
    TCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTAT11.0No Hit
    GGCACCTTCTTTAGTCAAATTCTCAGTGCCACAAAATTCGCAAGTGGCTT11.0No Hit
    ATTAACTATTAACGTACCTGTCTCTTCCGAAACGAATGAGTACATAAGTT11.0No Hit
    GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT11.0No Hit
    CAATTTTACTATCTGCATCTACAACCTGTTGGATTTCCCACAATGCTGAT11.0No Hit
    GGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGC11.0No Hit
    CACGATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATT11.0No Hit
    GTGCATTACCATGGACTTGACAATACAGATCATGGTTGCTTTGTAGGTTA11.0No Hit
    GTTGTAGTAAGCTAACGCATTGTCATCAGTGCAAGCAGTTTGTGTAGTAC11.0No Hit
    GCATTTCTTCACAAAGCTTGTTTATGTCTACAGCACCCTGCATGGAAAGC11.0No Hit
    TGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGT11.0No Hit
    TCATAGATGTCAACTCAAAGCCATGTGCCCATAAGACAAATACGACTCTG11.0No Hit
    ATAACACCCTTATAAAACATTTTAAAGCATTGAGCTGATTTGTCTTTATG11.0No Hit
    GGTCAAGGTTAATATAGGCATTAACAATGAATAATAAGAATCTACAACAG11.0No Hit
    TCTATAGGTCATGTCCTTAGGTATGCCAGGTATGTCAACACATAAACCTT11.0No Hit
    CTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTCTTCGC11.0No Hit
    CTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAA11.0No Hit
    TGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTT11.0No Hit
    CACAATTAGTGATTGGTTGTCCCCCACTAGCTAGATAATCTTTGTAAGCT11.0No Hit
    CATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTC11.0No Hit
    TGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTG11.0No Hit
    GGTTAAAGTCATTAAGAGTTGTGGTAAATCGATTGAGAAACCACCTGTCT11.0No Hit
    TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT11.0No Hit
    CAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAA11.0No Hit
    CAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCAT11.0No Hit
    GCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC11.0No Hit
    GTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGAT11.0No Hit
    GTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCT11.0No Hit
    GCATGAAGCTTTACCAGCACGTGCTAGAAGGTCTTTAATGCACTCAAGAG11.0No Hit
    AGCTACATGTGCATTACCATGGACTTGACAATACAGATCATGGTTGCTTT11.0No Hit
    TCTTTGTTAGCACCATAGGGAAGTCCAGATTCTGGCCCAGGTCCTAGGTA11.0No Hit
    CTTTCGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAAC11.0No Hit
    ATATTGCAGCAGTACGCACACAATCGAAGCGCAGTAAGGATGGCTAGTGT11.0No Hit
    GTCCACACCCAAATTATTGAGTATTTTCACCTCTGGTACTGGTTTAATGT11.0No Hit
    ATGAATCTGATGAATACATAGCTACTAATGGACCTCTTAAAGTGCGTGGT11.0No Hit
    ATACTTAGGTGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTACAC11.0No Hit
    ACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTAT11.0No Hit
    GCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATT11.0No Hit
    AGCTAACACTGAAAAAGTCTGTCCTGGTTGAATGCGAACAAACTTATACT11.0No Hit
    TTTCTGTACAAACAACACCATCCAATTTATAAGTAACTGGTTTTATGGTT11.0No Hit
    TATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCT11.0No Hit
    CGTGTAAGGAATTCTCTTACCACGCCTATTTGTGGCCTGTTAATTGCAGA11.0No Hit
    GTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTC11.0No Hit
    CCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTT11.0No Hit
    GATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAA11.0No Hit
    TTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATT11.0No Hit
    ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG11.0No Hit
    ATCATGACGTTCGTGTTGTTTTAGATTTCATCGAAACGAACAAACAAAAA11.0No Hit
    TTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTATCTTAG11.0No Hit
    GTTGTAAACACCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTT11.0No Hit
    CATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTT11.0No Hit
    GTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACA11.0No Hit
    ATACTGCGTCAAGGTTCACCGCACTCACTCAACATGCCAAGGAAGACATA11.0No Hit
    ATTATAGGATATTCAATAGTCCAGTCAACACGCTTAACAAAGCACTCGTG11.0No Hit
    TGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACA11.0No Hit
    GTACAAAAATAGCCTAAGAAACAATAAACTAGCATTATACACTGAAGTGT11.0No Hit
    TGTACACATAGTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCA11.0No Hit
    ATCTAAAGTCATTTGACTTAGGCCACGAGCTTGGCACTGATCCTTATGAA11.0No Hit
    TGTCATAATACGCATCACCCAACTAGCAGGCATATAGACCATATTAAAAT11.0No Hit
    GTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAA11.0No Hit
    GCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACAC11.0No Hit
    GTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTG11.0No Hit
    TGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCA11.0No Hit
    ATAGACAACAGGTGCGCTCAGGTCCTATTTTCACAAAATACTTCATAGAT11.0No Hit
    CTTGGTTAGCATTAATACAGCCACCATCGTAACAATCAAAGTACTTATCA11.0No Hit
    CATTTGGTGACGCAACTGGATAGACAGATCGAATTCTACCCATAAAGCCA11.0No Hit
    ATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTC11.0No Hit

    [OK]Adapter Content

    Adapter graph

    Produced by FastQC (version 0.12.1)
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zjsN~&@#off%yH*7joXH?*pB-5KGlyWk$+8=CBH_oo=ch-aIzZjA3Xsn0D@J|test_3.gz FastQC Report
    FastQCFastQC Report
    Fri 2 Feb 2024
    test_3.gz

    [OK]Basic Statistics

    MeasureValue
    Filenametest_3.gz
    File typeConventional base calls
    EncodingSanger / Illumina 1.9
    Total Sequences100
    Total Bases13.8 kbp
    Sequences flagged as poor quality0
    Sequence length72-151
    %GC39

    [OK]Per base sequence quality

    Per base quality graph

    [FAIL]Per tile sequence quality

    Per tile quality graph

    [OK]Per sequence quality scores

    Per Sequence quality graph

    [FAIL]Per base sequence content

    Per base sequence content

    [FAIL]Per sequence GC content

    Per sequence GC content graph

    [OK]Per base N content

    N content graph

    [WARN]Sequence Length Distribution

    Sequence length distribution

    [OK]Sequence Duplication Levels

    Duplication level graph

    [FAIL]Overrepresented sequences

    SequenceCountPercentagePossible Source
    CAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGA22.0No Hit
    ACTCTAGATAATTCATTAGGTTTCTTAATAGTAAGACTAGAATTGTCTAC11.0No Hit
    GTCTAATCATAATTTCTTGGTACAGGCTGGTATTGTTCATCTCAGGGTTA11.0No Hit
    TGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTA11.0No Hit
    TTTCAAGACTTGTAAATTGCAACTTGTCATAAAGGTCTCTATCAGACATT11.0No Hit
    TTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTA11.0No Hit
    ATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTA11.0No Hit
    GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT11.0No Hit
    AAGTGTATCTTGATCCTCATAACTCATTGAATCATAATAAAGTCTAGCCT11.0No Hit
    GTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTC11.0No Hit
    AATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTA11.0No Hit
    CAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAAT11.0No Hit
    GTTCCTAGGTAGTAGAAATACCATCTAGGACTGAGATCTTTCATTTTACA11.0No Hit
    CATCTTATTTCTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGA11.0No Hit
    TATCTGCCCATTTAATAGAAGTTAAACCATTAACTTGTGGGTATTTCCAC11.0No Hit
    CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT11.0No Hit
    ACTGAATTTTCTGCACCAAGTGACATAGTGTAGGCAATGATGGATTGACT11.0No Hit
    AACTAGCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGC11.0No Hit
    AAGTTGGCGTATACGCGTAATATATCTGGGTTTTCTACAAAATCATACCA11.0No Hit
    TTCCAGAAGAGCAAGGTTCTTTTAAAAGTACTGTTGTACCTCTAACACAC11.0No Hit
    TATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAA11.0No Hit
    TTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTAATGGGTAA11.0No Hit
    GGTGTATTCAAGGCTCCCTCAGTTGCAACCCATATGATGCCGTCTTTGTT11.0No Hit
    GTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTA11.0No Hit
    AAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTT11.0No Hit
    ACATAGTGTAGGCAATGATGGATTGACTAGCTACACTACGTGCCCGCCGA11.0No Hit
    GTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAA11.0No Hit
    CAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCT11.0No Hit
    GGATAGACAGATCGAATTCTACCCATAAAGCCATCAAGCTTTTTCTTTTC11.0No Hit
    ACTGTTTTCTTTGTAGAAAACATCCGTAATAGGACCTTTGTATTCTGAGG11.0No Hit
    GATGAAACATCATGCGTGATAACACCCTTATAAAACATTTTAAAGCATTG11.0No Hit
    GTTATAACACTGACACCACCAAAAGAACATGGTGTAATGTCAAGAATCTC11.0No Hit
    GCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGT11.0No Hit
    CCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGAC11.0No Hit
    CAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCT11.0No Hit
    CATTAGCACAAGTTGTAGGTATTTGTACATACTTACCTTTTAAGTCACAA11.0No Hit
    GTTACTCACAATTTTGACTTCACTTTTAGTCTTAGTCCAGAGTACTCAAT11.0No Hit
    TGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTG11.0No Hit
    CTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTG11.0No Hit
    TCTTTGTAAGCTTTAGCAGCATCTACAGCAAAAGCACAGAAAGATAATAC11.0No Hit
    ATGTCAGATTTATGTGACAATTTAAGACATTCAACAACATCTTTAGTTTC11.0No Hit
    GGATTAGTAACACTACAGCTGATGCATACATAACACAGTCTTTTAGCTTA11.0No Hit
    CTGTAATAGTTGTGTCCGTACCAGCTGCTTGTGCTGTTTGCCTGTCAACA11.0No Hit
    GAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTG11.0No Hit
    CTTTTACAAGACTTCAGAGTTTAGAAATTGTGGCTTATAATGTTGTAATT11.0No Hit
    TGGCAATAGAGTTATTAGAGTAAGCAACTGAATTTTCTGCACCAAGTGAC11.0No Hit
    CACAAACTCTAAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCAGAT11.0No Hit
    CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGA11.0No Hit
    AACACCAGCTTCTGATCTTTCACAAGTGCCGTGCCTACAGTACTCAGAAT11.0No Hit
    GATTTTTAAGTGTGTCACTTAACATTTGTACAATCTTTATACGCACTACA11.0No Hit
    AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC11.0No Hit
    AGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTA11.0No Hit
    CATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTT11.0No Hit
    GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG11.0No Hit
    ACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAA11.0No Hit
    CACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATA11.0No Hit
    TGCCAATCCTGTAGCGACTGTATGCAGCAAAACCTGAGTCACCTGCTACA11.0No Hit
    GATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGC11.0No Hit
    GTACGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTA11.0No Hit
    CTATAACATTAATAGGCAATGAACCTTTAGTGTTATTAGCTCTCAGGTTG11.0No Hit
    ATAAACATGTTAGGGTAACCATTAACTTGATAATTCATTTTAAAACCCAT11.0No Hit
    CCAAGCAGGGTTACGTGTAAGGAATTCTCTTACCACGCCTATTTGTGGCC11.0No Hit
    GATTTAGCCACATTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGA11.0No Hit
    GTTGAAAGTAGATCTTCAATAAATGACCTCTTGCTTGGTTTTGATGGATC11.0No Hit
    TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCG11.0No Hit
    TAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCG11.0No Hit
    GCTATAACGCAGCCTGTAAAATCATCTGGTAATTTATAATTATAATCAGC11.0No Hit
    AGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTT11.0No Hit
    TCTAAGCATAGTGAAAAGCATTGTCTGCATAGCACTAGTAACTTTTGCCC11.0No Hit
    GAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGA11.0No Hit
    ATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGAT11.0No Hit
    CCGTCTATGCAATACAAAGTTTCTTTAGAAGTTATATGTTTATAGTGACC11.0No Hit
    GCAATTCATAGCTCTTTTCAGAACGTTCCGTGTACCAAGCAATTTCATGC11.0No Hit
    AGTAAACTGTTACAAACAACAACAGCAAGTTGCAAACAAAGTGAACACCC11.0No Hit
    GTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAG11.0No Hit
    GTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTG11.0No Hit
    CTCCAAAGCCACGTACGAGCACGTCGCGAACCTGTAAAACAGGCAAACTG11.0No Hit
    GTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCA11.0No Hit
    GTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAATTGTCTGT11.0No Hit
    GTACAAAAATAGCCTAAGAAACAATAAACTAGCATTATACACTGAAGTGT11.0No Hit
    GATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACA11.0No Hit
    GTGAAATTAGTATGGACAAATCACCTAATTTAGCATGGCCTCTTATTGTA11.0No Hit
    TCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAG11.0No Hit
    GCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGC11.0No Hit
    TGCGTCTTGGGTCCCCGCTCTCACTCAACATGGCAAGGAAGACCTTAAAT11.0No Hit
    AACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCA11.0No Hit
    CTAAAAGTGAAGTCAAAATTGTGAGTAACAACCAGTGGTGTGTACCCGTG11.0No Hit
    ATGTAAGGCTTTGTTAAGTCAGTGTCAACATGTGACTCTGCAGTTAAAGC11.0No Hit
    CCTTGAATGAGTCTAATTCAGGTTGCAAAGGATCATAAACTGTGTTGTTG11.0No Hit
    GCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGC11.0No Hit
    ATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGG11.0No Hit
    GCTGCTAGAGACCTCGTTTGTGCACAAAAGTTTAACGGCCTTACTGTTTT11.0No Hit
    CCCTTTGTTGTGTTGTAGTAAGCTAACGCATTGTCATCAGTGCAAGCAGT11.0No Hit
    ATCCTTTAGGATTTGGATGATCTATGTGGCAACGGCAGTACAGACAACAC11.0No Hit
    CTACACGAGCACGTGCAGGTATAATTCTACTACATTTATCTATAGGCAAA11.0No Hit
    TGCCTGACCAGTACCAGTGTGTGTACACAACATCTTAACACAATTAGTGA11.0No Hit
    GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT11.0No Hit
    ATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTT11.0No Hit
    TTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATA11.0No Hit

    [OK]Adapter Content

    Adapter graph

    Produced by FastQC (version 0.12.1)
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zUI}{A00s8$i$71V-Os?lV!?=JemM&xTEaz7aTw-fasTA}oInX+iVjIxOl43b^9yq3=DaR3=A45 z;cm*yj6J0!rX&`W0LyMXse9*R;E^HH3=HR{qnjEpN~Eb++=_fPg93_M7aO9R3a!_R zQVa5nO7t>Ha&wStEKqVl9%@9bjWc`M(0q<|xdXaU$Zaan*dzixzlGf>;7DbFH!HC6 R2DJoa8ES#$q~|jb7XW1@45$DA literal 0 HcmV?d00001 diff --git a/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/null/fastqc/test_4_fastqc.html b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/null/fastqc/test_4_fastqc.html new file mode 100644 index 0000000..d102cbe --- /dev/null +++ b/.nf-test/tests/2ce7ef286c8e1e86a5e7a243a738e962/null/fastqc/test_4_fastqc.html @@ -0,0 +1,187 @@ +test_4.gz FastQC Report

    Summary

    [OK]Basic Statistics

    MeasureValue
    Filenametest_4.gz
    File typeConventional base calls
    EncodingSanger / Illumina 1.9
    Total Sequences100
    Total Bases13.7 kbp
    Sequences flagged as poor quality0
    Sequence length53-151
    %GC39

    [OK]Per base sequence quality

    Per base quality graph

    [FAIL]Per tile sequence quality

    Per tile quality graph

    [OK]Per sequence quality scores

    Per Sequence quality graph

    [FAIL]Per base sequence content

    Per base sequence content

    [FAIL]Per sequence GC content

    Per sequence GC content graph

    [OK]Per base N content

    N content graph

    [WARN]Sequence Length Distribution

    Sequence length distribution

    [OK]Sequence Duplication Levels

    Duplication level graph

    [FAIL]Overrepresented sequences

    SequenceCountPercentagePossible Source
    CAATAGTCCAGTCAACACGCTTAACAAAGCACTCGTGGACAGCTAGACAC22.0No Hit
    ATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTA11.0No Hit
    CCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCC11.0No Hit
    TTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGC11.0No Hit
    ATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGATGCGAGAGACTTGT11.0No Hit
    CTTAGGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTACACAATTT11.0No Hit
    GTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAAT11.0No Hit
    CCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATA11.0No Hit
    TTGTCATCAGTGCAAGCAGTTTGTGTAGTACCGGCAGCACAAGACATCTG11.0No Hit
    CCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTG11.0No Hit
    GTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTT11.0No Hit
    ATTATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGG11.0No Hit
    GCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACG11.0No Hit
    TCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGAT11.0No Hit
    CATAGTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCATAATTT11.0No Hit
    ACATACTGTTCTAATGTTGAAGTCACTTTGAATTTATCAAAACACTCTAC11.0No Hit
    CTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGT11.0No Hit
    CCCTTGGAGAGTGCTAGTTGCCATCTCTTTTTGAGGGTTATGATTTTGGA11.0No Hit
    GTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGACATGT11.0No Hit
    CCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGT11.0No Hit
    GGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCAT11.0No Hit
    ATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTA11.0No Hit
    TTAGTAGCCGTATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGTA11.0No Hit
    GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG11.0No Hit
    TGATCAACCTATCAATTTGCACTTCAGCCTCAACTTTGTCAAGACGTGAA11.0No Hit
    CTGTGGATCACGGACAGCATCAGTAGTGTCAGCAATGTCTCTGCCAAATT11.0No Hit
    GGTGGCTGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATC11.0No Hit
    CTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG11.0No Hit
    CTCAGTTGCAACCCATATGATGCCGTCTTTGTTAGCACCATAGGGAAGTC11.0No Hit
    GCGTACAACCAAGCTAAAGTTACTGGCCATAACAGCCAGAGGAAAATTAA11.0No Hit
    CCGCTAACAGTGCAGAAGTGTATTGAGCAATCATTTCATCTGTGAGCAAA11.0No Hit
    AGATAATAGATGGTAGAATGTAAAAGGCACTTTTACACTTTTTAAGCACT11.0No Hit
    TCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTAT11.0No Hit
    GGCACCTTCTTTAGTCAAATTCTCAGTGCCACAAAATTCGCAAGTGGCTT11.0No Hit
    ATTAACTATTAACGTACCTGTCTCTTCCGAAACGAATGAGTACATAAGTT11.0No Hit
    GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT11.0No Hit
    CAATTTTACTATCTGCATCTACAACCTGTTGGATTTCCCACAATGCTGAT11.0No Hit
    GGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGC11.0No Hit
    CACGATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATT11.0No Hit
    GTGCATTACCATGGACTTGACAATACAGATCATGGTTGCTTTGTAGGTTA11.0No Hit
    GTTGTAGTAAGCTAACGCATTGTCATCAGTGCAAGCAGTTTGTGTAGTAC11.0No Hit
    GCATTTCTTCACAAAGCTTGTTTATGTCTACAGCACCCTGCATGGAAAGC11.0No Hit
    TGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGT11.0No Hit
    TCATAGATGTCAACTCAAAGCCATGTGCCCATAAGACAAATACGACTCTG11.0No Hit
    ATAACACCCTTATAAAACATTTTAAAGCATTGAGCTGATTTGTCTTTATG11.0No Hit
    GGTCAAGGTTAATATAGGCATTAACAATGAATAATAAGAATCTACAACAG11.0No Hit
    TCTATAGGTCATGTCCTTAGGTATGCCAGGTATGTCAACACATAAACCTT11.0No Hit
    CTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTCTTCGC11.0No Hit
    CTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAA11.0No Hit
    TGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTT11.0No Hit
    CACAATTAGTGATTGGTTGTCCCCCACTAGCTAGATAATCTTTGTAAGCT11.0No Hit
    CATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTC11.0No Hit
    TGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTG11.0No Hit
    GGTTAAAGTCATTAAGAGTTGTGGTAAATCGATTGAGAAACCACCTGTCT11.0No Hit
    TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT11.0No Hit
    CAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAA11.0No Hit
    CAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCAT11.0No Hit
    GCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC11.0No Hit
    GTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGAT11.0No Hit
    GTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCT11.0No Hit
    GCATGAAGCTTTACCAGCACGTGCTAGAAGGTCTTTAATGCACTCAAGAG11.0No Hit
    AGCTACATGTGCATTACCATGGACTTGACAATACAGATCATGGTTGCTTT11.0No Hit
    TCTTTGTTAGCACCATAGGGAAGTCCAGATTCTGGCCCAGGTCCTAGGTA11.0No Hit
    CTTTCGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAAC11.0No Hit
    ATATTGCAGCAGTACGCACACAATCGAAGCGCAGTAAGGATGGCTAGTGT11.0No Hit
    GTCCACACCCAAATTATTGAGTATTTTCACCTCTGGTACTGGTTTAATGT11.0No Hit
    ATGAATCTGATGAATACATAGCTACTAATGGACCTCTTAAAGTGCGTGGT11.0No Hit
    ATACTTAGGTGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTACAC11.0No Hit
    ACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTAT11.0No Hit
    GCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATT11.0No Hit
    AGCTAACACTGAAAAAGTCTGTCCTGGTTGAATGCGAACAAACTTATACT11.0No Hit
    TTTCTGTACAAACAACACCATCCAATTTATAAGTAACTGGTTTTATGGTT11.0No Hit
    TATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCT11.0No Hit
    CGTGTAAGGAATTCTCTTACCACGCCTATTTGTGGCCTGTTAATTGCAGA11.0No Hit
    GTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTC11.0No Hit
    CCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTT11.0No Hit
    GATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAA11.0No Hit
    TTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATT11.0No Hit
    ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG11.0No Hit
    ATCATGACGTTCGTGTTGTTTTAGATTTCATCGAAACGAACAAACAAAAA11.0No Hit
    TTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTATCTTAG11.0No Hit
    GTTGTAAACACCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTT11.0No Hit
    CATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTT11.0No Hit
    GTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACA11.0No Hit
    ATACTGCGTCAAGGTTCACCGCACTCACTCAACATGCCAAGGAAGACATA11.0No Hit
    ATTATAGGATATTCAATAGTCCAGTCAACACGCTTAACAAAGCACTCGTG11.0No Hit
    TGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACA11.0No Hit
    GTACAAAAATAGCCTAAGAAACAATAAACTAGCATTATACACTGAAGTGT11.0No Hit
    TGTACACATAGTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCA11.0No Hit
    ATCTAAAGTCATTTGACTTAGGCCACGAGCTTGGCACTGATCCTTATGAA11.0No Hit
    TGTCATAATACGCATCACCCAACTAGCAGGCATATAGACCATATTAAAAT11.0No Hit
    GTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAA11.0No Hit
    GCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACAC11.0No Hit
    GTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTG11.0No Hit
    TGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCA11.0No Hit
    ATAGACAACAGGTGCGCTCAGGTCCTATTTTCACAAAATACTTCATAGAT11.0No Hit
    CTTGGTTAGCATTAATACAGCCACCATCGTAACAATCAAAGTACTTATCA11.0No Hit
    CATTTGGTGACGCAACTGGATAGACAGATCGAATTCTACCCATAAAGCCA11.0No Hit
    ATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTC11.0No Hit

    [OK]Adapter Content

    Adapter graph

    Produced by FastQC (version 0.12.1)
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"attachments": [ + { + "contentType": "application/vnd.microsoft.card.adaptive", + "contentUrl": null, + "content": { + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "msteams": { + "width": "Full" + }, + "type": "AdaptiveCard", + "version": "1.2", + "body": [ + { + "type": "TextBlock", + "size": "Large", + "weight": "Bolder", + "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", + "text": "nf-core/readsimulator v${version} - ${runName}", + "wrap": true + }, + { + "type": "TextBlock", + "spacing": "None", + "text": "Completed at ${dateComplete} (duration: ${duration})", + "isSubtle": true, + "wrap": true + }, + { + "type": "TextBlock", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors. The full error message was: ${errorReport}.<% } %>", + "wrap": true + }, + { + "type": "TextBlock", + "text": "The command used to launch the workflow was as follows:", + "wrap": true + }, + { + "type": "TextBlock", + "text": "${commandLine}", + "isSubtle": true, + "wrap": true + } + ], + "actions": [ + { + "type": "Action.ShowCard", + "title": "Pipeline Configuration", + "card": { + "type": "AdaptiveCard", + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "body": [ + { + "type": "FactSet", + "facts": [<% out << summary.collect{ k,v -> "{\"title\": \"$k\", \"value\" : \"$v\"}"}.join(",\n") %> + ] + } + ] + } + } + ] + } + } + ] +} diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/email_template.html b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/email_template.html new file mode 100644 index 0000000..f2bc049 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/email_template.html @@ -0,0 +1,53 @@ + + + + + + + + nf-core/readsimulator Pipeline Report + + +

     + + + diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/email_template.txt b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/email_template.txt new file mode 100644 index 0000000..6ca5507 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/email_template.txt @@ -0,0 +1,39 @@ +---------------------------------------------------- + ,--./,-. + ___ __ __ __ ___ /,-._.--~\\ + |\\ | |__ __ / ` / \\ |__) |__ } { + | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, + `._,._,' + nf-core/readsimulator ${version} +---------------------------------------------------- +Run Name: $runName + +<% if (success){ + out << "## nf-core/readsimulator execution completed successfully! ##" +} else { + out << """#################################################### +## nf-core/readsimulator execution completed unsuccessfully! ## +#################################################### +The exit status of the task that caused the workflow execution to fail was: $exitStatus. +The full error message was: + +${errorReport} +""" +} %> + + +The workflow was completed at $dateComplete (duration: $duration) + +The command used to launch the workflow was as follows: + + $commandLine + + + +Pipeline Configuration: +----------------------- +<% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> + +-- +nf-core/readsimulator +https://github.com/nf-core/readsimulator diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/methods_description_template.yml b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/methods_description_template.yml new file mode 100644 index 0000000..6119677 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/methods_description_template.yml @@ -0,0 +1,29 @@ +id: "nf-core-readsimulator-methods-description" +description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." +section_name: "nf-core/readsimulator Methods Description" +section_href: "https://github.com/nf-core/readsimulator" +plot_type: "html" +## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline +## You inject any metadata in the Nextflow '${workflow}' object +data: | +

    Methods

    +

    Data was processed using nf-core/readsimulator v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

    +

    The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

    + 
    ${workflow.commandLine}
    +

    ${tool_citations}

    +

    References

    +
      +
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
      • +
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
      • +
    • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
      • +
    • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
      • + ${tool_bibliography} +
    +
    +
    Notes:
    +
      + ${nodoi_text} +
    • The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
      • +
    • You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.
      • +
    +
    diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/multiqc_config.yml b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/multiqc_config.yml new file mode 100644 index 0000000..7d3650d --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/multiqc_config.yml @@ -0,0 +1,15 @@ +report_comment: > + + This report has been generated by the nf-core/readsimulator + analysis pipeline. 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charset=utf-8 + +$email_html + +--nfcoremimeboundary +Content-Type: image/png;name="nf-core-readsimulator_logo.png" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: inline; filename="nf-core-readsimulator_logo_light.png" + +<% out << new File("$projectDir/assets/nf-core-readsimulator_logo_light.png"). + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' ) %> + +<% +if (mqcFile){ +def mqcFileObj = new File("$mqcFile") +if (mqcFileObj.length() < mqcMaxSize){ +out << """ +--nfcoremimeboundary +Content-Type: text/html; name=\"multiqc_report\" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" + +${mqcFileObj. + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} +""" +}} +%> + +--nfcoremimeboundary-- diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/slackreport.json b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/slackreport.json new file mode 100644 index 0000000..e9d5f8a --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/assets/slackreport.json @@ -0,0 +1,34 @@ +{ + "attachments": [ + { + "fallback": "Plain-text summary of the attachment.", + "color": "<% if (success) { %>good<% } else { %>danger<%} %>", + "author_name": "nf-core/readsimulator ${version} - ${runName}", + "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", + "fields": [ + { + "title": "Command used to launch the workflow", + "value": "```${commandLine}```", + "short": false + } + <% + if (!success) { %> + , + { + "title": "Full error message", + "value": "```${errorReport}```", + "short": false + }, + { + "title": "Pipeline configuration", + "value": "<% out << summary.collect{ k,v -> k == "hook_url" ? "_${k}_: (_hidden_)" : ( ( v.class.toString().contains('Path') || ( v.class.toString().contains('String') && v.contains('/') ) ) ? "_${k}_: `${v}`" : (v.class.toString().contains('DateTime') ? ("_${k}_: " + v.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM))) : "_${k}_: ${v}") ) }.join(",\n") %>", + "short": false + } + <% } + %> + ], + "footer": "Completed at <% out << dateComplete.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM)) %> (duration: ${duration})" + } + ] +} diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/bin/check_samplesheet.py b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/bin/check_samplesheet.py new file mode 100755 index 0000000..4a758fe --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/bin/check_samplesheet.py @@ -0,0 +1,259 @@ +#!/usr/bin/env python + + +"""Provide a command line tool to validate and transform tabular samplesheets.""" + + +import argparse +import csv +import logging +import sys +from collections import Counter +from pathlib import Path + +logger = logging.getLogger() + + +class RowChecker: + """ + Define a service that can validate and transform each given row. + + Attributes: + modified (list): A list of dicts, where each dict corresponds to a previously + validated and transformed row. The order of rows is maintained. + + """ + + VALID_FORMATS = ( + ".fq.gz", + ".fastq.gz", + ) + + def __init__( + self, + sample_col="sample", + first_col="fastq_1", + second_col="fastq_2", + single_col="single_end", + **kwargs, + ): + """ + Initialize the row checker with the expected column names. + + Args: + sample_col (str): The name of the column that contains the sample name + (default "sample"). + first_col (str): The name of the column that contains the first (or only) + FASTQ file path (default "fastq_1"). + second_col (str): The name of the column that contains the second (if any) + FASTQ file path (default "fastq_2"). + single_col (str): The name of the new column that will be inserted and + records whether the sample contains single- or paired-end sequencing + reads (default "single_end"). + + """ + super().__init__(**kwargs) + self._sample_col = sample_col + self._first_col = first_col + self._second_col = second_col + self._single_col = single_col + self._seen = set() + self.modified = [] + + def validate_and_transform(self, row): + """ + Perform all validations on the given row and insert the read pairing status. + + Args: + row (dict): A mapping from column headers (keys) to elements of that row + (values). + + """ + self._validate_sample(row) + self._validate_first(row) + self._validate_second(row) + self._validate_pair(row) + self._seen.add((row[self._sample_col], row[self._first_col])) + self.modified.append(row) + + def _validate_sample(self, row): + """Assert that the sample name exists and convert spaces to underscores.""" + if len(row[self._sample_col]) <= 0: + raise AssertionError("Sample input is required.") + # Sanitize samples slightly. + row[self._sample_col] = row[self._sample_col].replace(" ", "_") + + def _validate_first(self, row): + """Assert that the first FASTQ entry is non-empty and has the right format.""" + if len(row[self._first_col]) <= 0: + raise AssertionError("At least the first FASTQ file is required.") + self._validate_fastq_format(row[self._first_col]) + + def _validate_second(self, row): + """Assert that the second FASTQ entry has the right format if it exists.""" + if len(row[self._second_col]) > 0: + self._validate_fastq_format(row[self._second_col]) + + def _validate_pair(self, row): + """Assert that read pairs have the same file extension. Report pair status.""" + if row[self._first_col] and row[self._second_col]: + row[self._single_col] = False + first_col_suffix = Path(row[self._first_col]).suffixes[-2:] + second_col_suffix = Path(row[self._second_col]).suffixes[-2:] + if first_col_suffix != second_col_suffix: + raise AssertionError("FASTQ pairs must have the same file extensions.") + else: + row[self._single_col] = True + + def _validate_fastq_format(self, filename): + """Assert that a given filename has one of the expected FASTQ extensions.""" + if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): + raise AssertionError( + f"The FASTQ file has an unrecognized extension: {filename}\n" + f"It should be one of: {', '.join(self.VALID_FORMATS)}" + ) + + def validate_unique_samples(self): + """ + Assert that the combination of sample name and FASTQ filename is unique. + + In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the + number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. + + """ + if len(self._seen) != len(self.modified): + raise AssertionError("The pair of sample name and FASTQ must be unique.") + seen = Counter() + for row in self.modified: + sample = row[self._sample_col] + seen[sample] += 1 + row[self._sample_col] = f"{sample}_T{seen[sample]}" + + +def read_head(handle, num_lines=10): + """Read the specified number of lines from the current position in the file.""" + lines = [] + for idx, line in enumerate(handle): + if idx == num_lines: + break + lines.append(line) + return "".join(lines) + + +def sniff_format(handle): + """ + Detect the tabular format. + + Args: + handle (text file): A handle to a `text file`_ object. The read position is + expected to be at the beginning (index 0). + + Returns: + csv.Dialect: The detected tabular format. + + .. _text file: + https://docs.python.org/3/glossary.html#term-text-file + + """ + peek = read_head(handle) + handle.seek(0) + sniffer = csv.Sniffer() + dialect = sniffer.sniff(peek) + return dialect + + +def check_samplesheet(file_in, file_out): + """ + Check that the tabular samplesheet has the structure expected by nf-core pipelines. + + Validate the general shape of the table, expected columns, and each row. Also add + an additional column which records whether one or two FASTQ reads were found. + + Args: + file_in (pathlib.Path): The given tabular samplesheet. The format can be either + CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. + file_out (pathlib.Path): Where the validated and transformed samplesheet should + be created; always in CSV format. + + Example: + This function checks that the samplesheet follows the following structure, + see also the `viral recon samplesheet`_:: + + sample,fastq_1,fastq_2 + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + + .. _viral recon samplesheet: + https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + + """ + required_columns = {"sample", "fastq_1", "fastq_2"} + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_in.open(newline="") as in_handle: + reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) + # Validate the existence of the expected header columns. + if not required_columns.issubset(reader.fieldnames): + req_cols = ", ".join(required_columns) + logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") + sys.exit(1) + # Validate each row. + checker = RowChecker() + for i, row in enumerate(reader): + try: + checker.validate_and_transform(row) + except AssertionError as error: + logger.critical(f"{str(error)} On line {i + 2}.") + sys.exit(1) + checker.validate_unique_samples() + header = list(reader.fieldnames) + header.insert(1, "single_end") + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_out.open(mode="w", newline="") as out_handle: + writer = csv.DictWriter(out_handle, header, delimiter=",") + writer.writeheader() + for row in checker.modified: + writer.writerow(row) + + +def parse_args(argv=None): + """Define and immediately parse command line arguments.""" + parser = argparse.ArgumentParser( + description="Validate and transform a tabular samplesheet.", + epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", + ) + parser.add_argument( + "file_in", + metavar="FILE_IN", + type=Path, + help="Tabular input samplesheet in CSV or TSV format.", + ) + parser.add_argument( + "file_out", + metavar="FILE_OUT", + type=Path, + help="Transformed output samplesheet in CSV format.", + ) + parser.add_argument( + "-l", + "--log-level", + help="The desired log level (default WARNING).", + choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), + default="WARNING", + ) + return parser.parse_args(argv) + + +def main(argv=None): + """Coordinate argument parsing and program execution.""" + args = parse_args(argv) + logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") + if not args.file_in.is_file(): + logger.error(f"The given input file {args.file_in} was not found!") + sys.exit(2) + args.file_out.parent.mkdir(parents=True, exist_ok=True) + check_samplesheet(args.file_in, args.file_out) + + +if __name__ == "__main__": + sys.exit(main()) diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/NfcoreTemplate.groovy b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/NfcoreTemplate.groovy new file mode 100755 index 0000000..e248e4c --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/NfcoreTemplate.groovy @@ -0,0 +1,356 @@ +// +// This file holds several functions used within the nf-core pipeline template. +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +class NfcoreTemplate { + + // + // Check AWS Batch related parameters have been specified correctly + // + public static void awsBatch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + // Check params.awsqueue and params.awsregion have been set if running on AWSBatch + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + } + } + + // + // Warn if a -profile or Nextflow config has not been provided to run the pipeline + // + public static void checkConfigProvided(workflow, log) { + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + } + } + + // + // Generate version string + // + public static String version(workflow) { + String version_string = "" + + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string + } + + // + // Construct and send completion email + // + public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = NfcoreTemplate.version(workflow) + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() + } + + // + // Construct and send a notification to a web server as JSON + // e.g. Microsoft Teams and Slack + // + public static void IM_notification(workflow, params, summary_params, projectDir, log) { + def hook_url = params.hook_url + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = NfcoreTemplate.version(workflow) + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("$projectDir/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } + } + + // + // Dump pipeline parameters in a json file + // + public static void dump_parameters(workflow, params) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() + } + + // + // Print pipeline summary on completion + // + public static void summary(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } + + // + // ANSII Colours used for terminal logging + // + public static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + // + // Does what is says on the tin + // + public static String dashedLine(monochrome_logs) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + // + // nf-core logo + // + public static String logo(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String workflow_version = NfcoreTemplate.version(workflow) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/NftestUtils.groovy b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/NftestUtils.groovy new file mode 100644 index 0000000..ce6fc8b --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/NftestUtils.groovy @@ -0,0 +1,38 @@ +// Helper functions for pipeline tests + +class NftestUtils { + + // Function to remove Nextflow version from software_versions.yml + public static String removeNextflowVersion(outputDir) { + def softwareVersions = path("$outputDir/pipeline_info/software_versions.yml").yaml + if (softwareVersions.containsKey("Workflow")) { + softwareVersions.Workflow.remove("Nextflow") + } + return softwareVersions + } + + // Function to filter lines from a file and return a new file + public static File filterLines(String inFilePath, int linesToSkip) { + if (linesToSkip >= 0) { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lineCount = 0 + inputFile.eachLine { line -> + lineCount++ + if (lineCount > linesToSkip) { + outputFile.append(line + '\n') + } + } + return outputFile + } else { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lines = inputFile.readLines() + def totalLines = lines.size() + lines.take(totalLines + linesToSkip).each { line -> + outputFile.append(line + '\n') + } + return outputFile + } + } +} diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/Utils.groovy b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/Utils.groovy new file mode 100644 index 0000000..8d030f4 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/Utils.groovy @@ -0,0 +1,47 @@ +// +// This file holds several Groovy functions that could be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml + +class Utils { + + // + // When running with -profile conda, warn if channels have not been set-up appropriately + // + public static void checkCondaChannels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } + } +} diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/WorkflowMain.groovy b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/WorkflowMain.groovy new file mode 100755 index 0000000..477ddaf --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/WorkflowMain.groovy @@ -0,0 +1,77 @@ +// +// This file holds several functions specific to the main.nf workflow in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow + +class WorkflowMain { + + // + // Citation string for pipeline + // + public static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + // TODO nf-core: Add Zenodo DOI for pipeline after first release + //"* The pipeline\n" + + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + + // + // Validate parameters and print summary to screen + // + public static void initialise(workflow, params, log, args) { + + // Print workflow version and exit on --version + if (params.version) { + String workflow_version = NfcoreTemplate.version(workflow) + log.info "${workflow.manifest.name} ${workflow_version}" + System.exit(0) + } + + // Check that a -profile or Nextflow config has been provided to run the pipeline + NfcoreTemplate.checkConfigProvided(workflow, log) + // Check that the profile doesn't contain spaces and doesn't end with a trailing comma + checkProfile(workflow.profile, args, log) + + // Check that conda channels are set-up correctly + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + Utils.checkCondaChannels(log) + } + + // Check AWS batch settings + NfcoreTemplate.awsBatch(workflow, params) + + // Check input has been provided + if (!params.input) { + Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") + } + } + // + // Get attribute from genome config file e.g. fasta + // + public static Object getGenomeAttribute(params, attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null + } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } +} diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/WorkflowReadsimulator.groovy b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/WorkflowReadsimulator.groovy new file mode 100755 index 0000000..c0b9df0 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib/WorkflowReadsimulator.groovy @@ -0,0 +1,131 @@ +// +// This file holds several functions specific to the workflow/readsimulator.nf in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow +import groovy.text.SimpleTemplateEngine + +class WorkflowReadsimulator { + + // + // Check and validate parameters + // + public static void initialise(params, log) { + + genomeExistsError(params, log) + + if (!params.fasta && !params.ncbidownload_accessions && !params.ncbidownload_taxids) { + Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file. Alternatively, '--ncbidownload_accessions accessions.txt' or '--ncbidownload_taxids taxids.txt' can be used." + } + } + + // + // Get workflow summary for MultiQC + // + public static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } + + // + // Generate methods description for MultiQC + // + + public static String toolCitationText(params) { + def citation_text = [ + "Tools used in the workflow included:", + params["amplicon"] ? [ + "CRABS (Jeunen et al. 2022),", + "ART (Huang et al. 2012)," + ].join(' ').trim() : "", + params["target_capture"] ? [ + "Bowtie2 (Langmead et al. 2012),", + "Samtools (Danecek et al. 2021),", + "CapSim (Cao et al. 2018)," + ].join(' ').trim() : "", + params["metagenome"] ? + "InSilicoSeq (Gourlé et al. 2018)," : "", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text + } + + public static String toolBibliographyText(params) { + def reference_text = [ + params["amplicon"] ? [ + "
  • Jeunen, G.-J., Dowle, E., Edgecombe, J., von Ammon, U., Gemmell, N. J., & Cross, H. (2022). crabs—A software program to generate curated reference databases for metabarcoding sequencing data. Molecular Ecology Resources, 00, 1– 14. https://doi.org/10.1111/1755-0998.13741
    • ", + "
  • Weichun Huang, Leping Li, Jason R. Myers, Gabor T. Marth, ART: a next-generation sequencing read simulator, Bioinformatics, Volume 28, Issue 4, February 2012, Pages 593–594, https://doi.org/10.1093/bioinformatics/btr708
    • " + ].join(' ').trim() : "", + params["target_capture"] ? [ + "
  • Langmead, B., Salzberg, S. Fast gapped-read alignment with Bowtie 2. Nat Methods 9, 357–359 (2012). https://doi.org/10.1038/nmeth.1923
    • ", + "
  • Twelve years of SAMtools and BCFtools. Petr Danecek, James K Bonfield, Jennifer Liddle, John Marshall, Valeriu Ohan, Martin O Pollard, Andrew Whitwham, Thomas Keane, Shane A McCarthy, Robert M Davies, Heng Li. GigaScience, Volume 10, Issue 2, February 2021, giab008, https://doi.org/10.1093/gigascience/giab008
    • ", + "
  • Minh Duc Cao, Devika Ganesamoorthy, Chenxi Zhou, Lachlan J M Coin, Simulating the dynamics of targeted capture sequencing with CapSim, Bioinformatics, Volume 34, Issue 5, March 2018, Pages 873–874, https://doi.org/10.1093/bioinformatics/btx691
    • " + ].join(' ').trim() : "", + params["metagenome"] ? + "
  • Gourlé H, Karlsson-Lindsjö O, Hayer J and Bongcam+Rudloff E, Simulating Illumina data with InSilicoSeq. Bioinformatics (2018) doi:10.1093/bioinformatics/bty630
    • " : "", + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text + } + + public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = run_workflow.toMap() + meta["manifest_map"] = run_workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + meta["tool_bibliography"] = toolBibliographyText(params) + + + def methods_text = mqc_methods_yaml.text + + def engine = new SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html + } + + // + // Exit pipeline if incorrect --genome key provided + // + private static void genomeExistsError(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + Nextflow.error(error_string) + } + } +} diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/mock.nf b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/mock.nf new file mode 100644 index 0000000..5a5696e --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/mock.nf @@ -0,0 +1,85 @@ +import groovy.json.JsonGenerator +import groovy.json.JsonGenerator.Converter + +nextflow.enable.dsl=2 + +// comes from nf-test to store json files +params.nf_test_output = "" + +// include dependencies + + +// include test process +include { FASTQC } from '/workspaces/readsimulator/./modules/nf-core/fastqc/tests/../main.nf' + +// define custom rules for JSON that will be generated. +def jsonOutput = + new JsonGenerator.Options() + .excludeNulls() // Do not include fields with value null.. + .addConverter(Path) { value -> value.toString() } // Custom converter for Path. Only filename + .build() + + +workflow { + + // run dependencies + + + // process mapping + def input = [] + + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + + //---- + + //run process + FASTQC(*input) + + if (FASTQC.output){ + + // consumes all named output channels and stores items in a json file + for (def name in FASTQC.out.getNames()) { + serializeChannel(name, FASTQC.out.getProperty(name), jsonOutput) + } + + // consumes all unnamed output channels and stores items in a json file + def array = FASTQC.out as Object[] + for (def i = 0; i < array.length ; i++) { + serializeChannel(i, array[i], jsonOutput) + } + + } + +} + +def serializeChannel(name, channel, jsonOutput) { + def _name = name + def list = [ ] + channel.subscribe( + onNext: { + list.add(it) + }, + onComplete: { + def map = new HashMap() + map[_name] = list + def filename = "${params.nf_test_output}/output_${_name}.json" + new File(filename).text = jsonOutput.toJson(map) + } + ) +} + + +workflow.onComplete { + + def result = [ + success: workflow.success, + exitStatus: workflow.exitStatus, + errorMessage: workflow.errorMessage, + errorReport: workflow.errorReport + ] + new File("${params.nf_test_output}/workflow.json").text = jsonOutput.toJson(result) + +} diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/nextflow.log b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/nextflow.log new file mode 100644 index 0000000..1527d86 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/nextflow.log @@ -0,0 +1,100 @@ +Feb-02 16:21:01.896 [main] DEBUG nextflow.cli.Launcher - $> nextflow -quiet -log /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/nextflow.log run /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/mock.nf -c /workspaces/readsimulator/nextflow.config -c /workspaces/readsimulator/tests/nextflow.config -params-file /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/params.json -ansi-log false -profile singularity -with-trace /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/trace.csv -w /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work -stub +Feb-02 16:21:02.020 [main] INFO nextflow.cli.CmdRun - N E X T F L O W ~ version 23.10.1 +Feb-02 16:21:02.057 [main] DEBUG nextflow.plugin.PluginsFacade - Setting up plugin manager > mode=prod; embedded=false; plugins-dir=/home/gitpod/.nextflow/plugins; core-plugins: nf-amazon@2.1.4,nf-azure@1.3.3,nf-cloudcache@0.3.0,nf-codecommit@0.1.5,nf-console@1.0.6,nf-ga4gh@1.1.0,nf-google@1.8.3,nf-tower@1.6.3,nf-wave@1.0.1 +Feb-02 16:21:02.075 [main] INFO o.pf4j.DefaultPluginStatusProvider - Enabled plugins: [] +Feb-02 16:21:02.076 [main] INFO o.pf4j.DefaultPluginStatusProvider - Disabled plugins: [] +Feb-02 16:21:02.081 [main] INFO org.pf4j.DefaultPluginManager - PF4J version 3.4.1 in 'deployment' mode +Feb-02 16:21:02.098 [main] INFO org.pf4j.AbstractPluginManager - No plugins +Feb-02 16:21:02.122 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /workspaces/readsimulator/nextflow.config +Feb-02 16:21:02.123 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /workspaces/readsimulator/tests/nextflow.config +Feb-02 16:21:02.124 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /workspaces/readsimulator/nextflow.config +Feb-02 16:21:02.124 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /workspaces/readsimulator/tests/nextflow.config +Feb-02 16:21:02.165 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity` +Feb-02 16:21:06.082 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity` +Feb-02 16:21:07.183 [main] DEBUG nextflow.config.ConfigBuilder - Available config profiles: [cfc_dev, uzl_omics, ifb_core, denbi_qbic, alice, mjolnir_globe, uppmax, incliva, ilifu, uge, rosalind_uge, lugh, unibe_ibu, vai, czbiohub_aws, jax, ccga_med, scw, tigem, tubingen_apg, google, apollo, ipop_up, pdc_kth, googlels, daisybio, eddie, medair, biowulf, apptainer, bi, bigpurple, adcra, cedars, pawsey_setonix, vsc_kul_uhasselt, pawsey_nimbus, ucl_myriad, utd_ganymede, charliecloud, icr_davros, ceres, munin, arm, rosalind, hasta, cfc, uzh, ebi_codon_slurm, ebc, ku_sund_dangpu, ccga_dx, crick, marvin, biohpc_gen, shifter, mana, mamba, unc_lccc, wehi, awsbatch, wustl_htcf, imperial, maestro, software_license, genotoul, nci_gadi, abims, janelia, nu_genomics, googlebatch, oist, sahmri, mpcdf, leicester, vsc_ugent, create, sage, cambridge, jex, podman, ebi_codon, cheaha, xanadu, nyu_hpc, test, computerome, ucd_sonic, seg_globe, sanger, dkfz, pasteur, ethz_euler, test_full, imb, tuos_stanage, azurebatch, hki, crukmi, csiro_petrichor, docker, engaging, gis, hypatia, psmn, eva, nygc, fgcz, conda, crg, singularity, self_hosted_runner, uw_hyak_pedslabs, utd_sysbio, debug, genouest, cbe, phoenix, gitpod, seawulf, uod_hpc, fub_curta, uct_hpc, aws_tower, binac] +Feb-02 16:21:07.260 [main] DEBUG nextflow.cli.CmdRun - Applied DSL=2 from script declararion +Feb-02 16:21:07.284 [main] INFO nextflow.cli.CmdRun - Launching `/workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/mock.nf` [backstabbing_ampere] DSL2 - revision: b9d7cbaea3 +Feb-02 16:21:07.284 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins declared=[nf-validation@1.1.3] +Feb-02 16:21:07.285 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins default=[] +Feb-02 16:21:07.285 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins resolved requirement=[nf-validation@1.1.3] +Feb-02 16:21:07.285 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-validation version: 1.1.3 +Feb-02 16:21:07.293 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-validation@1.1.3' resolved +Feb-02 16:21:07.294 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-validation@1.1.3' +Feb-02 16:21:07.307 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-validation@1.1.3 +Feb-02 16:21:07.320 [main] DEBUG n.secret.LocalSecretsProvider - Secrets store: /home/gitpod/.nextflow/secrets/store.json +Feb-02 16:21:07.325 [main] DEBUG nextflow.secret.SecretsLoader - Discovered secrets providers: [nextflow.secret.LocalSecretsProvider@6cdbe5ec] - activable => nextflow.secret.LocalSecretsProvider@6cdbe5ec +Feb-02 16:21:07.434 [main] DEBUG nextflow.Session - Session UUID: c3e119cb-7457-470f-aa33-bf5743e74f87 +Feb-02 16:21:07.434 [main] DEBUG nextflow.Session - Run name: backstabbing_ampere +Feb-02 16:21:07.435 [main] DEBUG nextflow.Session - Executor pool size: 8 +Feb-02 16:21:07.446 [main] DEBUG nextflow.file.FilePorter - File porter settings maxRetries=3; maxTransfers=50; pollTimeout=null +Feb-02 16:21:07.452 [main] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'FileTransfer' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:21:07.482 [main] DEBUG nextflow.cli.CmdRun - + Version: 23.10.1 build 5891 + Created: 12-01-2024 22:01 UTC (22:01 GMT) + System: Linux 5.15.133.1-microsoft-standard-WSL2 + Runtime: Groovy 3.0.19 on OpenJDK 64-Bit Server VM 17.0.10-internal+0-adhoc..src + Encoding: UTF-8 (UTF-8) + Process: 19710@b23599a75663 [172.17.0.3] + CPUs: 8 - Mem: 7.6 GB (477.3 MB) - Swap: 2 GB (2 GB) +Feb-02 16:21:07.520 [main] DEBUG nextflow.Session - Work-dir: /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work [ext2/ext3] +Feb-02 16:21:07.539 [main] DEBUG nextflow.executor.ExecutorFactory - Extension executors providers=[] +Feb-02 16:21:07.552 [main] DEBUG nextflow.Session - Observer factory: DefaultObserverFactory +Feb-02 16:21:07.638 [main] DEBUG nextflow.cache.CacheFactory - Using Nextflow cache factory: nextflow.cache.DefaultCacheFactory +Feb-02 16:21:07.651 [main] DEBUG nextflow.util.CustomThreadPool - Creating default thread pool > poolSize: 9; maxThreads: 1000 +Feb-02 16:21:07.766 [main] DEBUG nextflow.Session - Session start +Feb-02 16:21:07.772 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow started -- trace file: /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/trace.csv +Feb-02 16:21:07.779 [main] DEBUG nextflow.Session - Using default localLib path: /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib +Feb-02 16:21:07.783 [main] DEBUG nextflow.Session - Adding to the classpath library: /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/lib +Feb-02 16:21:08.200 [main] DEBUG nextflow.script.ScriptRunner - > Launching execution +Feb-02 16:21:08.561 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-amazon version: 2.1.4 +Feb-02 16:21:08.570 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-amazon@2.1.4' resolved +Feb-02 16:21:08.570 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-amazon@2.1.4' +Feb-02 16:21:08.594 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-amazon@2.1.4 +Feb-02 16:21:08.615 [main] DEBUG nextflow.file.FileHelper - > Added 'S3FileSystemProvider' to list of installed providers [s3] +Feb-02 16:21:08.615 [main] DEBUG nextflow.file.FileHelper - Started plugin 'nf-amazon' required to handle file: s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_1.fastq.gz +Feb-02 16:21:08.626 [main] DEBUG nextflow.file.FileHelper - Creating a file system instance for provider: S3FileSystemProvider +Feb-02 16:21:08.649 [main] DEBUG nextflow.cloud.aws.config.AwsConfig - AWS S3 config properties: {max_error_retry=5} +Feb-02 16:21:09.328 [main] DEBUG nextflow.cloud.aws.nio.S3Client - Setting S3 glacierRetrievalTier=null +Feb-02 16:21:09.403 [main] DEBUG n.c.aws.util.S3CredentialsProvider - No AWS credentials available - falling back to anonymous access +Feb-02 16:21:11.316 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name FASTQC +Feb-02 16:21:11.318 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:FASTQC` matches process FASTQC +Feb-02 16:21:11.319 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process FASTQC +Feb-02 16:21:11.328 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Feb-02 16:21:11.329 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Feb-02 16:21:11.337 [main] DEBUG nextflow.executor.Executor - [warm up] executor > local +Feb-02 16:21:11.344 [main] DEBUG n.processor.LocalPollingMonitor - Creating local task monitor for executor 'local' > cpus=8; memory=7.6 GB; capacity=8; pollInterval=100ms; dumpInterval=5m +Feb-02 16:21:11.347 [main] DEBUG n.processor.TaskPollingMonitor - >>> barrier register (monitor: local) +Feb-02 16:21:11.478 [main] DEBUG nextflow.Session - Config process names validation disabled as requested +Feb-02 16:21:11.478 [main] DEBUG nextflow.Session - Igniting dataflow network (2) +Feb-02 16:21:11.479 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > FASTQC +Feb-02 16:21:11.480 [main] DEBUG nextflow.script.ScriptRunner - Parsed script files: + Script_eca2c974a292664e: /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/mock.nf + Script_4ba798786ecd6418: /workspaces/readsimulator/./modules/nf-core/fastqc/tests/../main.nf +Feb-02 16:21:11.480 [main] DEBUG nextflow.script.ScriptRunner - > Awaiting termination +Feb-02 16:21:11.480 [main] DEBUG nextflow.Session - Session await +Feb-02 16:21:11.770 [FileTransfer-1] DEBUG nextflow.file.FilePorter - Copying foreign file s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_1.fastq.gz to work dir: /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work/stage-c3e119cb-7457-470f-aa33-bf5743e74f87/f8/d5386a169b9ef8415115463cad2063/test_1.fastq.gz +Feb-02 16:21:11.972 [FileTransfer-1] DEBUG n.cloud.aws.nio.S3FileSystemProvider - S3 download file from=s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_1.fastq.gz to=/workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work/stage-c3e119cb-7457-470f-aa33-bf5743e74f87/f8/d5386a169b9ef8415115463cad2063/test_1.fastq.gz +Feb-02 16:21:11.972 [FileTransfer-1] DEBUG nextflow.cloud.aws.nio.S3Client - Creating S3 transfer manager pool - chunk-size=104857600; max-treads=10; +Feb-02 16:21:11.972 [FileTransfer-1] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'S3TransferManager' minSize=10; maxSize=10; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:21:12.607 [Actor Thread 7] WARN nextflow.container.SingularityCache - Singularity cache directory has not been defined -- Remote image will be stored in the path: /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work/singularity -- Use the environment variable NXF_SINGULARITY_CACHEDIR to specify a different location +Feb-02 16:21:12.608 [Actor Thread 7] INFO nextflow.container.SingularityCache - Pulling Singularity image https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0 [cache /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work/singularity/depot.galaxyproject.org-singularity-fastqc-0.12.1--hdfd78af_0.img] +Feb-02 16:21:13.330 [Actor Thread 7] DEBUG nextflow.container.SingularityCache - Singularity pull complete image=https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0 path=/workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work/singularity/depot.galaxyproject.org-singularity-fastqc-0.12.1--hdfd78af_0.img +Feb-02 16:21:13.435 [Task submitter] DEBUG n.executor.local.LocalTaskHandler - Launch cmd line: /bin/bash -ue .command.run +Feb-02 16:21:13.437 [Task submitter] INFO nextflow.Session - [05/4a82b6] Submitted process > FASTQC (test) +Feb-02 16:21:15.118 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[id: 1; name: FASTQC (test); status: COMPLETED; exit: 0; error: -; workDir: /workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work/05/4a82b6ece2f6f45392ad4e4b764481] +Feb-02 16:21:15.163 [Task monitor] WARN nextflow.processor.PublishDir - Process `FASTQC` publishDir path contains a variable with a null value +Feb-02 16:21:15.176 [Task monitor] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'PublishDir' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:21:15.186 [main] DEBUG nextflow.Session - Session await > all processes finished +Feb-02 16:21:15.188 [Task monitor] DEBUG n.processor.TaskPollingMonitor - <<< barrier arrives (monitor: local) - terminating tasks monitor poll loop +Feb-02 16:21:15.189 [main] DEBUG nextflow.Session - Session await > all barriers passed +Feb-02 16:21:15.191 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'PublishDir' shutdown completed (hard=false) +Feb-02 16:21:15.202 [main] DEBUG n.trace.WorkflowStatsObserver - Workflow completed > WorkflowStats[succeededCount=1; failedCount=0; ignoredCount=0; cachedCount=0; pendingCount=0; submittedCount=0; runningCount=0; retriesCount=0; abortedCount=0; succeedDuration=4s; failedDuration=0ms; cachedDuration=0ms;loadCpus=0; loadMemory=0; peakRunning=1; peakCpus=2; peakMemory=3 GB; ] +Feb-02 16:21:15.202 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow completed -- saving trace file +Feb-02 16:21:15.267 [main] DEBUG nextflow.cache.CacheDB - Closing CacheDB done +Feb-02 16:21:15.267 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-amazon@2.1.4' +Feb-02 16:21:15.268 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-amazon +Feb-02 16:21:15.268 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-validation@1.1.3' +Feb-02 16:21:15.268 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-validation +Feb-02 16:21:15.287 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'FileTransfer' shutdown completed (hard=false) +Feb-02 16:21:15.287 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'S3TransferManager' shutdown completed (hard=false) +Feb-02 16:21:15.287 [main] DEBUG nextflow.script.ScriptRunner - > Execution complete -- Goodbye diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_0.json b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_0.json new file mode 100644 index 0000000..5d82ee0 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_0.json @@ -0,0 +1 @@ +{"0":[[{"id":"test","single_end":true},"/workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work/05/4a82b6ece2f6f45392ad4e4b764481/test.html"]]} \ No newline at end of file diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_1.json b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_1.json new file mode 100644 index 0000000..b6057b9 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_1.json @@ -0,0 +1 @@ +{"1":[[{"id":"test","single_end":true},"/workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work/05/4a82b6ece2f6f45392ad4e4b764481/test.zip"]]} \ No newline at end of file diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_2.json b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_2.json new file mode 100644 index 0000000..36e1ba9 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_2.json @@ -0,0 +1 @@ +{"2":["/workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work/05/4a82b6ece2f6f45392ad4e4b764481/versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_html.json b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_html.json new file mode 100644 index 0000000..5027fcc --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_html.json @@ -0,0 +1 @@ +{"html":[[{"id":"test","single_end":true},"/workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work/05/4a82b6ece2f6f45392ad4e4b764481/test.html"]]} \ No newline at end of file diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_versions.json b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_versions.json new file mode 100644 index 0000000..9323197 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_versions.json @@ -0,0 +1 @@ +{"versions":["/workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work/05/4a82b6ece2f6f45392ad4e4b764481/versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_zip.json b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_zip.json new file mode 100644 index 0000000..47dc243 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/output_zip.json @@ -0,0 +1 @@ +{"zip":[[{"id":"test","single_end":true},"/workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/work/05/4a82b6ece2f6f45392ad4e4b764481/test.zip"]]} \ No newline at end of file diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/params.json b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/params.json new file mode 100644 index 0000000..96e6143 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/params.json @@ -0,0 +1 @@ +{"nf_test_output":"/workspaces/readsimulator/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta"} \ No newline at end of file diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/std.err b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/std.err new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/std.out b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/std.out new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/trace.csv b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/trace.csv new file mode 100644 index 0000000..bf5bfb1 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/trace.csv @@ -0,0 +1,2 @@ +task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar +1 05/4a82b6 19897 FASTQC (test) COMPLETED 0 2024-02-02 16:21:13.437 1.7s 2s 41.0% 49.4 MB 3.2 GB 1.3 MB 271 B diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/workflow.json b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/workflow.json new file mode 100644 index 0000000..7d54df0 --- /dev/null +++ b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/meta/workflow.json @@ -0,0 +1 @@ +{"success":true,"exitStatus":0} \ No newline at end of file diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/null/fastqc/test.html b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/null/fastqc/test.html new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/null/fastqc/test.zip b/.nf-test/tests/312eb30fba21aa2c15e8fab9da0b797/null/fastqc/test.zip new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/adaptivecard.json b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/adaptivecard.json new file mode 100644 index 0000000..dbf3df9 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/adaptivecard.json @@ -0,0 +1,67 @@ +{ + "type": "message", + "attachments": [ + { + "contentType": "application/vnd.microsoft.card.adaptive", + "contentUrl": null, + "content": { + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "msteams": { + "width": "Full" + }, + "type": "AdaptiveCard", + "version": "1.2", + "body": [ + { + "type": "TextBlock", + "size": "Large", + "weight": "Bolder", + "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", + "text": "nf-core/readsimulator v${version} - ${runName}", + "wrap": true + }, + { + "type": "TextBlock", + "spacing": "None", + "text": "Completed at ${dateComplete} (duration: ${duration})", + "isSubtle": true, + "wrap": true + }, + { + "type": "TextBlock", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors. The full error message was: ${errorReport}.<% } %>", + "wrap": true + }, + { + "type": "TextBlock", + "text": "The command used to launch the workflow was as follows:", + "wrap": true + }, + { + "type": "TextBlock", + "text": "${commandLine}", + "isSubtle": true, + "wrap": true + } + ], + "actions": [ + { + "type": "Action.ShowCard", + "title": "Pipeline Configuration", + "card": { + "type": "AdaptiveCard", + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "body": [ + { + "type": "FactSet", + "facts": [<% out << summary.collect{ k,v -> "{\"title\": \"$k\", \"value\" : \"$v\"}"}.join(",\n") %> + ] + } + ] + } + } + ] + } + } + ] +} diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/email_template.html b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/email_template.html new file mode 100644 index 0000000..f2bc049 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/email_template.html @@ -0,0 +1,53 @@ + + + + + + + + nf-core/readsimulator Pipeline Report + + +
    + + + +

    nf-core/readsimulator ${version}

    +

    Run Name: $runName

    + +<% if (!success){ + out << """ +
    +

    nf-core/readsimulator execution completed unsuccessfully!

    +

    The exit status of the task that caused the workflow execution to fail was: $exitStatus.

    +

    The full error message was:

    + 
    ${errorReport}
    +
    + """ +} else { + out << """ +
    + nf-core/readsimulator execution completed successfully! +
    + """ +} +%> + +

    The workflow was completed at $dateComplete (duration: $duration)

    +

    The command used to launch the workflow was as follows:

    +
    $commandLine
    + +

    Pipeline Configuration:

    + + + <% out << summary.collect{ k,v -> "" }.join("\n") %> + +
    $k
    $v
    + +

    nf-core/readsimulator

    +

    https://github.com/nf-core/readsimulator

    + +
    + + + diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/email_template.txt b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/email_template.txt new file mode 100644 index 0000000..6ca5507 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/email_template.txt @@ -0,0 +1,39 @@ +---------------------------------------------------- + ,--./,-. + ___ __ __ __ ___ /,-._.--~\\ + |\\ | |__ __ / ` / \\ |__) |__ } { + | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, + `._,._,' + nf-core/readsimulator ${version} +---------------------------------------------------- +Run Name: $runName + +<% if (success){ + out << "## nf-core/readsimulator execution completed successfully! ##" +} else { + out << """#################################################### +## nf-core/readsimulator execution completed unsuccessfully! ## +#################################################### +The exit status of the task that caused the workflow execution to fail was: $exitStatus. +The full error message was: + +${errorReport} +""" +} %> + + +The workflow was completed at $dateComplete (duration: $duration) + +The command used to launch the workflow was as follows: + + $commandLine + + + +Pipeline Configuration: +----------------------- +<% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> + +-- +nf-core/readsimulator +https://github.com/nf-core/readsimulator diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/methods_description_template.yml b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/methods_description_template.yml new file mode 100644 index 0000000..6119677 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/methods_description_template.yml @@ -0,0 +1,29 @@ +id: "nf-core-readsimulator-methods-description" +description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." +section_name: "nf-core/readsimulator Methods Description" +section_href: "https://github.com/nf-core/readsimulator" +plot_type: "html" +## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline +## You inject any metadata in the Nextflow '${workflow}' object +data: | +

    Methods

    +

    Data was processed using nf-core/readsimulator v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

    +

    The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

    + 
    ${workflow.commandLine}
    +

    ${tool_citations}

    +

    References

    +
      +
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
      • +
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
      • +
    • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
      • +
    • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
      • + ${tool_bibliography} +
    +
    +
    Notes:
    +
      + ${nodoi_text} +
    • The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
      • +
    • You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.
      • +
    +
    diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/multiqc_config.yml b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/multiqc_config.yml new file mode 100644 index 0000000..7d3650d --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/multiqc_config.yml @@ -0,0 +1,15 @@ +report_comment: > + + This report has been generated by the nf-core/readsimulator + analysis pipeline. 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charset=utf-8 + +$email_html + +--nfcoremimeboundary +Content-Type: image/png;name="nf-core-readsimulator_logo.png" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: inline; filename="nf-core-readsimulator_logo_light.png" + +<% out << new File("$projectDir/assets/nf-core-readsimulator_logo_light.png"). + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' ) %> + +<% +if (mqcFile){ +def mqcFileObj = new File("$mqcFile") +if (mqcFileObj.length() < mqcMaxSize){ +out << """ +--nfcoremimeboundary +Content-Type: text/html; name=\"multiqc_report\" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" + +${mqcFileObj. + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} +""" +}} +%> + +--nfcoremimeboundary-- diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/slackreport.json b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/slackreport.json new file mode 100644 index 0000000..e9d5f8a --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/assets/slackreport.json @@ -0,0 +1,34 @@ +{ + "attachments": [ + { + "fallback": "Plain-text summary of the attachment.", + "color": "<% if (success) { %>good<% } else { %>danger<%} %>", + "author_name": "nf-core/readsimulator ${version} - ${runName}", + "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", + "fields": [ + { + "title": "Command used to launch the workflow", + "value": "```${commandLine}```", + "short": false + } + <% + if (!success) { %> + , + { + "title": "Full error message", + "value": "```${errorReport}```", + "short": false + }, + { + "title": "Pipeline configuration", + "value": "<% out << summary.collect{ k,v -> k == "hook_url" ? "_${k}_: (_hidden_)" : ( ( v.class.toString().contains('Path') || ( v.class.toString().contains('String') && v.contains('/') ) ) ? "_${k}_: `${v}`" : (v.class.toString().contains('DateTime') ? ("_${k}_: " + v.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM))) : "_${k}_: ${v}") ) }.join(",\n") %>", + "short": false + } + <% } + %> + ], + "footer": "Completed at <% out << dateComplete.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM)) %> (duration: ${duration})" + } + ] +} diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/bin/check_samplesheet.py b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/bin/check_samplesheet.py new file mode 100755 index 0000000..4a758fe --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/bin/check_samplesheet.py @@ -0,0 +1,259 @@ +#!/usr/bin/env python + + +"""Provide a command line tool to validate and transform tabular samplesheets.""" + + +import argparse +import csv +import logging +import sys +from collections import Counter +from pathlib import Path + +logger = logging.getLogger() + + +class RowChecker: + """ + Define a service that can validate and transform each given row. + + Attributes: + modified (list): A list of dicts, where each dict corresponds to a previously + validated and transformed row. The order of rows is maintained. + + """ + + VALID_FORMATS = ( + ".fq.gz", + ".fastq.gz", + ) + + def __init__( + self, + sample_col="sample", + first_col="fastq_1", + second_col="fastq_2", + single_col="single_end", + **kwargs, + ): + """ + Initialize the row checker with the expected column names. + + Args: + sample_col (str): The name of the column that contains the sample name + (default "sample"). + first_col (str): The name of the column that contains the first (or only) + FASTQ file path (default "fastq_1"). + second_col (str): The name of the column that contains the second (if any) + FASTQ file path (default "fastq_2"). + single_col (str): The name of the new column that will be inserted and + records whether the sample contains single- or paired-end sequencing + reads (default "single_end"). + + """ + super().__init__(**kwargs) + self._sample_col = sample_col + self._first_col = first_col + self._second_col = second_col + self._single_col = single_col + self._seen = set() + self.modified = [] + + def validate_and_transform(self, row): + """ + Perform all validations on the given row and insert the read pairing status. + + Args: + row (dict): A mapping from column headers (keys) to elements of that row + (values). + + """ + self._validate_sample(row) + self._validate_first(row) + self._validate_second(row) + self._validate_pair(row) + self._seen.add((row[self._sample_col], row[self._first_col])) + self.modified.append(row) + + def _validate_sample(self, row): + """Assert that the sample name exists and convert spaces to underscores.""" + if len(row[self._sample_col]) <= 0: + raise AssertionError("Sample input is required.") + # Sanitize samples slightly. + row[self._sample_col] = row[self._sample_col].replace(" ", "_") + + def _validate_first(self, row): + """Assert that the first FASTQ entry is non-empty and has the right format.""" + if len(row[self._first_col]) <= 0: + raise AssertionError("At least the first FASTQ file is required.") + self._validate_fastq_format(row[self._first_col]) + + def _validate_second(self, row): + """Assert that the second FASTQ entry has the right format if it exists.""" + if len(row[self._second_col]) > 0: + self._validate_fastq_format(row[self._second_col]) + + def _validate_pair(self, row): + """Assert that read pairs have the same file extension. Report pair status.""" + if row[self._first_col] and row[self._second_col]: + row[self._single_col] = False + first_col_suffix = Path(row[self._first_col]).suffixes[-2:] + second_col_suffix = Path(row[self._second_col]).suffixes[-2:] + if first_col_suffix != second_col_suffix: + raise AssertionError("FASTQ pairs must have the same file extensions.") + else: + row[self._single_col] = True + + def _validate_fastq_format(self, filename): + """Assert that a given filename has one of the expected FASTQ extensions.""" + if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): + raise AssertionError( + f"The FASTQ file has an unrecognized extension: {filename}\n" + f"It should be one of: {', '.join(self.VALID_FORMATS)}" + ) + + def validate_unique_samples(self): + """ + Assert that the combination of sample name and FASTQ filename is unique. + + In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the + number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. + + """ + if len(self._seen) != len(self.modified): + raise AssertionError("The pair of sample name and FASTQ must be unique.") + seen = Counter() + for row in self.modified: + sample = row[self._sample_col] + seen[sample] += 1 + row[self._sample_col] = f"{sample}_T{seen[sample]}" + + +def read_head(handle, num_lines=10): + """Read the specified number of lines from the current position in the file.""" + lines = [] + for idx, line in enumerate(handle): + if idx == num_lines: + break + lines.append(line) + return "".join(lines) + + +def sniff_format(handle): + """ + Detect the tabular format. + + Args: + handle (text file): A handle to a `text file`_ object. The read position is + expected to be at the beginning (index 0). + + Returns: + csv.Dialect: The detected tabular format. + + .. _text file: + https://docs.python.org/3/glossary.html#term-text-file + + """ + peek = read_head(handle) + handle.seek(0) + sniffer = csv.Sniffer() + dialect = sniffer.sniff(peek) + return dialect + + +def check_samplesheet(file_in, file_out): + """ + Check that the tabular samplesheet has the structure expected by nf-core pipelines. + + Validate the general shape of the table, expected columns, and each row. Also add + an additional column which records whether one or two FASTQ reads were found. + + Args: + file_in (pathlib.Path): The given tabular samplesheet. The format can be either + CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. + file_out (pathlib.Path): Where the validated and transformed samplesheet should + be created; always in CSV format. + + Example: + This function checks that the samplesheet follows the following structure, + see also the `viral recon samplesheet`_:: + + sample,fastq_1,fastq_2 + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + + .. _viral recon samplesheet: + https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + + """ + required_columns = {"sample", "fastq_1", "fastq_2"} + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_in.open(newline="") as in_handle: + reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) + # Validate the existence of the expected header columns. + if not required_columns.issubset(reader.fieldnames): + req_cols = ", ".join(required_columns) + logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") + sys.exit(1) + # Validate each row. + checker = RowChecker() + for i, row in enumerate(reader): + try: + checker.validate_and_transform(row) + except AssertionError as error: + logger.critical(f"{str(error)} On line {i + 2}.") + sys.exit(1) + checker.validate_unique_samples() + header = list(reader.fieldnames) + header.insert(1, "single_end") + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_out.open(mode="w", newline="") as out_handle: + writer = csv.DictWriter(out_handle, header, delimiter=",") + writer.writeheader() + for row in checker.modified: + writer.writerow(row) + + +def parse_args(argv=None): + """Define and immediately parse command line arguments.""" + parser = argparse.ArgumentParser( + description="Validate and transform a tabular samplesheet.", + epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", + ) + parser.add_argument( + "file_in", + metavar="FILE_IN", + type=Path, + help="Tabular input samplesheet in CSV or TSV format.", + ) + parser.add_argument( + "file_out", + metavar="FILE_OUT", + type=Path, + help="Transformed output samplesheet in CSV format.", + ) + parser.add_argument( + "-l", + "--log-level", + help="The desired log level (default WARNING).", + choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), + default="WARNING", + ) + return parser.parse_args(argv) + + +def main(argv=None): + """Coordinate argument parsing and program execution.""" + args = parse_args(argv) + logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") + if not args.file_in.is_file(): + logger.error(f"The given input file {args.file_in} was not found!") + sys.exit(2) + args.file_out.parent.mkdir(parents=True, exist_ok=True) + check_samplesheet(args.file_in, args.file_out) + + +if __name__ == "__main__": + sys.exit(main()) diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/NfcoreTemplate.groovy b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/NfcoreTemplate.groovy new file mode 100755 index 0000000..e248e4c --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/NfcoreTemplate.groovy @@ -0,0 +1,356 @@ +// +// This file holds several functions used within the nf-core pipeline template. +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +class NfcoreTemplate { + + // + // Check AWS Batch related parameters have been specified correctly + // + public static void awsBatch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + // Check params.awsqueue and params.awsregion have been set if running on AWSBatch + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + } + } + + // + // Warn if a -profile or Nextflow config has not been provided to run the pipeline + // + public static void checkConfigProvided(workflow, log) { + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + } + } + + // + // Generate version string + // + public static String version(workflow) { + String version_string = "" + + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string + } + + // + // Construct and send completion email + // + public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = NfcoreTemplate.version(workflow) + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() + } + + // + // Construct and send a notification to a web server as JSON + // e.g. Microsoft Teams and Slack + // + public static void IM_notification(workflow, params, summary_params, projectDir, log) { + def hook_url = params.hook_url + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = NfcoreTemplate.version(workflow) + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("$projectDir/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } + } + + // + // Dump pipeline parameters in a json file + // + public static void dump_parameters(workflow, params) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() + } + + // + // Print pipeline summary on completion + // + public static void summary(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } + + // + // ANSII Colours used for terminal logging + // + public static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + // + // Does what is says on the tin + // + public static String dashedLine(monochrome_logs) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + // + // nf-core logo + // + public static String logo(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String workflow_version = NfcoreTemplate.version(workflow) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/NftestUtils.groovy b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/NftestUtils.groovy new file mode 100644 index 0000000..ce6fc8b --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/NftestUtils.groovy @@ -0,0 +1,38 @@ +// Helper functions for pipeline tests + +class NftestUtils { + + // Function to remove Nextflow version from software_versions.yml + public static String removeNextflowVersion(outputDir) { + def softwareVersions = path("$outputDir/pipeline_info/software_versions.yml").yaml + if (softwareVersions.containsKey("Workflow")) { + softwareVersions.Workflow.remove("Nextflow") + } + return softwareVersions + } + + // Function to filter lines from a file and return a new file + public static File filterLines(String inFilePath, int linesToSkip) { + if (linesToSkip >= 0) { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lineCount = 0 + inputFile.eachLine { line -> + lineCount++ + if (lineCount > linesToSkip) { + outputFile.append(line + '\n') + } + } + return outputFile + } else { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lines = inputFile.readLines() + def totalLines = lines.size() + lines.take(totalLines + linesToSkip).each { line -> + outputFile.append(line + '\n') + } + return outputFile + } + } +} diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/Utils.groovy b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/Utils.groovy new file mode 100644 index 0000000..8d030f4 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/Utils.groovy @@ -0,0 +1,47 @@ +// +// This file holds several Groovy functions that could be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml + +class Utils { + + // + // When running with -profile conda, warn if channels have not been set-up appropriately + // + public static void checkCondaChannels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } + } +} diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/WorkflowMain.groovy b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/WorkflowMain.groovy new file mode 100755 index 0000000..477ddaf --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/WorkflowMain.groovy @@ -0,0 +1,77 @@ +// +// This file holds several functions specific to the main.nf workflow in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow + +class WorkflowMain { + + // + // Citation string for pipeline + // + public static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + // TODO nf-core: Add Zenodo DOI for pipeline after first release + //"* The pipeline\n" + + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + + // + // Validate parameters and print summary to screen + // + public static void initialise(workflow, params, log, args) { + + // Print workflow version and exit on --version + if (params.version) { + String workflow_version = NfcoreTemplate.version(workflow) + log.info "${workflow.manifest.name} ${workflow_version}" + System.exit(0) + } + + // Check that a -profile or Nextflow config has been provided to run the pipeline + NfcoreTemplate.checkConfigProvided(workflow, log) + // Check that the profile doesn't contain spaces and doesn't end with a trailing comma + checkProfile(workflow.profile, args, log) + + // Check that conda channels are set-up correctly + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + Utils.checkCondaChannels(log) + } + + // Check AWS batch settings + NfcoreTemplate.awsBatch(workflow, params) + + // Check input has been provided + if (!params.input) { + Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") + } + } + // + // Get attribute from genome config file e.g. fasta + // + public static Object getGenomeAttribute(params, attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null + } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } +} diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/WorkflowReadsimulator.groovy b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/WorkflowReadsimulator.groovy new file mode 100755 index 0000000..c0b9df0 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib/WorkflowReadsimulator.groovy @@ -0,0 +1,131 @@ +// +// This file holds several functions specific to the workflow/readsimulator.nf in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow +import groovy.text.SimpleTemplateEngine + +class WorkflowReadsimulator { + + // + // Check and validate parameters + // + public static void initialise(params, log) { + + genomeExistsError(params, log) + + if (!params.fasta && !params.ncbidownload_accessions && !params.ncbidownload_taxids) { + Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file. Alternatively, '--ncbidownload_accessions accessions.txt' or '--ncbidownload_taxids taxids.txt' can be used." + } + } + + // + // Get workflow summary for MultiQC + // + public static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } + + // + // Generate methods description for MultiQC + // + + public static String toolCitationText(params) { + def citation_text = [ + "Tools used in the workflow included:", + params["amplicon"] ? [ + "CRABS (Jeunen et al. 2022),", + "ART (Huang et al. 2012)," + ].join(' ').trim() : "", + params["target_capture"] ? [ + "Bowtie2 (Langmead et al. 2012),", + "Samtools (Danecek et al. 2021),", + "CapSim (Cao et al. 2018)," + ].join(' ').trim() : "", + params["metagenome"] ? + "InSilicoSeq (Gourlé et al. 2018)," : "", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text + } + + public static String toolBibliographyText(params) { + def reference_text = [ + params["amplicon"] ? [ + "
  • Jeunen, G.-J., Dowle, E., Edgecombe, J., von Ammon, U., Gemmell, N. J., & Cross, H. (2022). crabs—A software program to generate curated reference databases for metabarcoding sequencing data. Molecular Ecology Resources, 00, 1– 14. https://doi.org/10.1111/1755-0998.13741
    • ", + "
  • Weichun Huang, Leping Li, Jason R. Myers, Gabor T. Marth, ART: a next-generation sequencing read simulator, Bioinformatics, Volume 28, Issue 4, February 2012, Pages 593–594, https://doi.org/10.1093/bioinformatics/btr708
    • " + ].join(' ').trim() : "", + params["target_capture"] ? [ + "
  • Langmead, B., Salzberg, S. Fast gapped-read alignment with Bowtie 2. Nat Methods 9, 357–359 (2012). https://doi.org/10.1038/nmeth.1923
    • ", + "
  • Twelve years of SAMtools and BCFtools. Petr Danecek, James K Bonfield, Jennifer Liddle, John Marshall, Valeriu Ohan, Martin O Pollard, Andrew Whitwham, Thomas Keane, Shane A McCarthy, Robert M Davies, Heng Li. GigaScience, Volume 10, Issue 2, February 2021, giab008, https://doi.org/10.1093/gigascience/giab008
    • ", + "
  • Minh Duc Cao, Devika Ganesamoorthy, Chenxi Zhou, Lachlan J M Coin, Simulating the dynamics of targeted capture sequencing with CapSim, Bioinformatics, Volume 34, Issue 5, March 2018, Pages 873–874, https://doi.org/10.1093/bioinformatics/btx691
    • " + ].join(' ').trim() : "", + params["metagenome"] ? + "
  • Gourlé H, Karlsson-Lindsjö O, Hayer J and Bongcam+Rudloff E, Simulating Illumina data with InSilicoSeq. Bioinformatics (2018) doi:10.1093/bioinformatics/bty630
    • " : "", + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text + } + + public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = run_workflow.toMap() + meta["manifest_map"] = run_workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + meta["tool_bibliography"] = toolBibliographyText(params) + + + def methods_text = mqc_methods_yaml.text + + def engine = new SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html + } + + // + // Exit pipeline if incorrect --genome key provided + // + private static void genomeExistsError(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + Nextflow.error(error_string) + } + } +} diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/mock.nf b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/mock.nf new file mode 100644 index 0000000..c20bd39 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/mock.nf @@ -0,0 +1,85 @@ +import groovy.json.JsonGenerator +import groovy.json.JsonGenerator.Converter + +nextflow.enable.dsl=2 + +// comes from nf-test to store json files +params.nf_test_output = "" + +// include dependencies + + +// include test process +include { FASTQC } from '/workspaces/readsimulator/./modules/nf-core/fastqc/tests/../main.nf' + +// define custom rules for JSON that will be generated. +def jsonOutput = + new JsonGenerator.Options() + .excludeNulls() // Do not include fields with value null.. + .addConverter(Path) { value -> value.toString() } // Custom converter for Path. Only filename + .build() + + +workflow { + + // run dependencies + + + // process mapping + def input = [] + + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + + //---- + + //run process + FASTQC(*input) + + if (FASTQC.output){ + + // consumes all named output channels and stores items in a json file + for (def name in FASTQC.out.getNames()) { + serializeChannel(name, FASTQC.out.getProperty(name), jsonOutput) + } + + // consumes all unnamed output channels and stores items in a json file + def array = FASTQC.out as Object[] + for (def i = 0; i < array.length ; i++) { + serializeChannel(i, array[i], jsonOutput) + } + + } + +} + +def serializeChannel(name, channel, jsonOutput) { + def _name = name + def list = [ ] + channel.subscribe( + onNext: { + list.add(it) + }, + onComplete: { + def map = new HashMap() + map[_name] = list + def filename = "${params.nf_test_output}/output_${_name}.json" + new File(filename).text = jsonOutput.toJson(map) + } + ) +} + + +workflow.onComplete { + + def result = [ + success: workflow.success, + exitStatus: workflow.exitStatus, + errorMessage: workflow.errorMessage, + errorReport: workflow.errorReport + ] + new File("${params.nf_test_output}/workflow.json").text = jsonOutput.toJson(result) + +} diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/nextflow.log b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/nextflow.log new file mode 100644 index 0000000..0358ff5 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/nextflow.log @@ -0,0 +1,100 @@ +Feb-02 16:20:02.618 [main] DEBUG nextflow.cli.Launcher - $> nextflow -quiet -log /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/nextflow.log run /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/mock.nf -c /workspaces/readsimulator/nextflow.config -c /workspaces/readsimulator/tests/nextflow.config -params-file /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/params.json -ansi-log false -profile singularity -with-trace /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/trace.csv -w /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work +Feb-02 16:20:02.707 [main] INFO nextflow.cli.CmdRun - N E X T F L O W ~ version 23.10.1 +Feb-02 16:20:02.735 [main] DEBUG nextflow.plugin.PluginsFacade - Setting up plugin manager > mode=prod; embedded=false; plugins-dir=/home/gitpod/.nextflow/plugins; core-plugins: nf-amazon@2.1.4,nf-azure@1.3.3,nf-cloudcache@0.3.0,nf-codecommit@0.1.5,nf-console@1.0.6,nf-ga4gh@1.1.0,nf-google@1.8.3,nf-tower@1.6.3,nf-wave@1.0.1 +Feb-02 16:20:02.754 [main] INFO o.pf4j.DefaultPluginStatusProvider - Enabled plugins: [] +Feb-02 16:20:02.755 [main] INFO o.pf4j.DefaultPluginStatusProvider - Disabled plugins: [] +Feb-02 16:20:02.763 [main] INFO org.pf4j.DefaultPluginManager - PF4J version 3.4.1 in 'deployment' mode +Feb-02 16:20:02.779 [main] INFO org.pf4j.AbstractPluginManager - No plugins +Feb-02 16:20:02.806 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /workspaces/readsimulator/nextflow.config +Feb-02 16:20:02.807 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /workspaces/readsimulator/tests/nextflow.config +Feb-02 16:20:02.808 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /workspaces/readsimulator/nextflow.config +Feb-02 16:20:02.808 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /workspaces/readsimulator/tests/nextflow.config +Feb-02 16:20:02.848 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity` +Feb-02 16:20:05.781 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity` +Feb-02 16:20:06.544 [main] DEBUG nextflow.config.ConfigBuilder - Available config profiles: [cfc_dev, uzl_omics, ifb_core, denbi_qbic, alice, mjolnir_globe, uppmax, incliva, ilifu, uge, rosalind_uge, lugh, unibe_ibu, vai, czbiohub_aws, jax, ccga_med, scw, tigem, tubingen_apg, google, apollo, ipop_up, pdc_kth, googlels, daisybio, eddie, medair, biowulf, apptainer, bi, bigpurple, adcra, cedars, pawsey_setonix, vsc_kul_uhasselt, pawsey_nimbus, ucl_myriad, utd_ganymede, charliecloud, icr_davros, ceres, munin, arm, rosalind, hasta, cfc, uzh, ebi_codon_slurm, ebc, ku_sund_dangpu, ccga_dx, crick, marvin, biohpc_gen, shifter, mana, mamba, unc_lccc, wehi, awsbatch, wustl_htcf, imperial, maestro, software_license, genotoul, nci_gadi, abims, janelia, nu_genomics, googlebatch, oist, sahmri, mpcdf, leicester, vsc_ugent, create, sage, cambridge, jex, podman, ebi_codon, cheaha, xanadu, nyu_hpc, test, computerome, ucd_sonic, seg_globe, sanger, dkfz, pasteur, ethz_euler, test_full, imb, tuos_stanage, azurebatch, hki, crukmi, csiro_petrichor, docker, engaging, gis, hypatia, psmn, eva, nygc, fgcz, conda, crg, singularity, self_hosted_runner, uw_hyak_pedslabs, utd_sysbio, debug, genouest, cbe, phoenix, gitpod, seawulf, uod_hpc, fub_curta, uct_hpc, aws_tower, binac] +Feb-02 16:20:06.593 [main] DEBUG nextflow.cli.CmdRun - Applied DSL=2 from script declararion +Feb-02 16:20:06.611 [main] INFO nextflow.cli.CmdRun - Launching `/workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/mock.nf` [amazing_lumiere] DSL2 - revision: 27312085ad +Feb-02 16:20:06.611 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins declared=[nf-validation@1.1.3] +Feb-02 16:20:06.611 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins default=[] +Feb-02 16:20:06.611 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins resolved requirement=[nf-validation@1.1.3] +Feb-02 16:20:06.612 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-validation version: 1.1.3 +Feb-02 16:20:06.618 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-validation@1.1.3' resolved +Feb-02 16:20:06.619 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-validation@1.1.3' +Feb-02 16:20:06.627 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-validation@1.1.3 +Feb-02 16:20:06.637 [main] DEBUG n.secret.LocalSecretsProvider - Secrets store: /home/gitpod/.nextflow/secrets/store.json +Feb-02 16:20:06.642 [main] DEBUG nextflow.secret.SecretsLoader - Discovered secrets providers: [nextflow.secret.LocalSecretsProvider@14b8a751] - activable => nextflow.secret.LocalSecretsProvider@14b8a751 +Feb-02 16:20:06.732 [main] DEBUG nextflow.Session - Session UUID: 1bf2003e-2ce7-4807-af2e-703d4e14c22b +Feb-02 16:20:06.733 [main] DEBUG nextflow.Session - Run name: amazing_lumiere +Feb-02 16:20:06.733 [main] DEBUG nextflow.Session - Executor pool size: 8 +Feb-02 16:20:06.742 [main] DEBUG nextflow.file.FilePorter - File porter settings maxRetries=3; maxTransfers=50; pollTimeout=null +Feb-02 16:20:06.749 [main] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'FileTransfer' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:20:06.780 [main] DEBUG nextflow.cli.CmdRun - + Version: 23.10.1 build 5891 + Created: 12-01-2024 22:01 UTC (22:01 GMT) + System: Linux 5.15.133.1-microsoft-standard-WSL2 + Runtime: Groovy 3.0.19 on OpenJDK 64-Bit Server VM 17.0.10-internal+0-adhoc..src + Encoding: UTF-8 (UTF-8) + Process: 17555@b23599a75663 [172.17.0.3] + CPUs: 8 - Mem: 7.6 GB (413.2 MB) - Swap: 2 GB (2 GB) +Feb-02 16:20:06.807 [main] DEBUG nextflow.Session - Work-dir: /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work [ext2/ext3] +Feb-02 16:20:06.822 [main] DEBUG nextflow.executor.ExecutorFactory - Extension executors providers=[] +Feb-02 16:20:06.832 [main] DEBUG nextflow.Session - Observer factory: DefaultObserverFactory +Feb-02 16:20:06.883 [main] DEBUG nextflow.cache.CacheFactory - Using Nextflow cache factory: nextflow.cache.DefaultCacheFactory +Feb-02 16:20:06.896 [main] DEBUG nextflow.util.CustomThreadPool - Creating default thread pool > poolSize: 9; maxThreads: 1000 +Feb-02 16:20:06.990 [main] DEBUG nextflow.Session - Session start +Feb-02 16:20:06.995 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow started -- trace file: /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/trace.csv +Feb-02 16:20:06.999 [main] DEBUG nextflow.Session - Using default localLib path: /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib +Feb-02 16:20:07.002 [main] DEBUG nextflow.Session - Adding to the classpath library: /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/lib +Feb-02 16:20:07.270 [main] DEBUG nextflow.script.ScriptRunner - > Launching execution +Feb-02 16:20:07.510 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-amazon version: 2.1.4 +Feb-02 16:20:07.517 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-amazon@2.1.4' resolved +Feb-02 16:20:07.518 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-amazon@2.1.4' +Feb-02 16:20:07.535 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-amazon@2.1.4 +Feb-02 16:20:07.556 [main] DEBUG nextflow.file.FileHelper - > Added 'S3FileSystemProvider' to list of installed providers [s3] +Feb-02 16:20:07.557 [main] DEBUG nextflow.file.FileHelper - Started plugin 'nf-amazon' required to handle file: s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam +Feb-02 16:20:07.567 [main] DEBUG nextflow.file.FileHelper - Creating a file system instance for provider: S3FileSystemProvider +Feb-02 16:20:07.585 [main] DEBUG nextflow.cloud.aws.config.AwsConfig - AWS S3 config properties: {max_error_retry=5} +Feb-02 16:20:08.145 [main] DEBUG nextflow.cloud.aws.nio.S3Client - Setting S3 glacierRetrievalTier=null +Feb-02 16:20:08.226 [main] DEBUG n.c.aws.util.S3CredentialsProvider - No AWS credentials available - falling back to anonymous access +Feb-02 16:20:10.184 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name FASTQC +Feb-02 16:20:10.186 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:FASTQC` matches process FASTQC +Feb-02 16:20:10.188 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process FASTQC +Feb-02 16:20:10.198 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Feb-02 16:20:10.198 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Feb-02 16:20:10.207 [main] DEBUG nextflow.executor.Executor - [warm up] executor > local +Feb-02 16:20:10.216 [main] DEBUG n.processor.LocalPollingMonitor - Creating local task monitor for executor 'local' > cpus=8; memory=7.6 GB; capacity=8; pollInterval=100ms; dumpInterval=5m +Feb-02 16:20:10.220 [main] DEBUG n.processor.TaskPollingMonitor - >>> barrier register (monitor: local) +Feb-02 16:20:10.316 [main] DEBUG nextflow.Session - Config process names validation disabled as requested +Feb-02 16:20:10.316 [main] DEBUG nextflow.Session - Igniting dataflow network (2) +Feb-02 16:20:10.316 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > FASTQC +Feb-02 16:20:10.318 [main] DEBUG nextflow.script.ScriptRunner - Parsed script files: + Script_feb5f9a56b9efed6: /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/mock.nf + Script_4ba798786ecd6418: /workspaces/readsimulator/./modules/nf-core/fastqc/tests/../main.nf +Feb-02 16:20:10.318 [main] DEBUG nextflow.script.ScriptRunner - > Awaiting termination +Feb-02 16:20:10.318 [main] DEBUG nextflow.Session - Session await +Feb-02 16:20:10.583 [FileTransfer-1] DEBUG nextflow.file.FilePorter - Copying foreign file s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam to work dir: /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work/stage-1bf2003e-2ce7-4807-af2e-703d4e14c22b/f3/6f83bdbb906f8ea7597fce74a000f2/test.paired_end.sorted.bam +Feb-02 16:20:10.789 [FileTransfer-1] DEBUG n.cloud.aws.nio.S3FileSystemProvider - S3 download file from=s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam to=/workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work/stage-1bf2003e-2ce7-4807-af2e-703d4e14c22b/f3/6f83bdbb906f8ea7597fce74a000f2/test.paired_end.sorted.bam +Feb-02 16:20:10.789 [FileTransfer-1] DEBUG nextflow.cloud.aws.nio.S3Client - Creating S3 transfer manager pool - chunk-size=104857600; max-treads=10; +Feb-02 16:20:10.790 [FileTransfer-1] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'S3TransferManager' minSize=10; maxSize=10; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:20:11.626 [Actor Thread 7] WARN nextflow.container.SingularityCache - Singularity cache directory has not been defined -- Remote image will be stored in the path: /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work/singularity -- Use the environment variable NXF_SINGULARITY_CACHEDIR to specify a different location +Feb-02 16:20:11.626 [Actor Thread 7] INFO nextflow.container.SingularityCache - Pulling Singularity image https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0 [cache /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work/singularity/depot.galaxyproject.org-singularity-fastqc-0.12.1--hdfd78af_0.img] +Feb-02 16:20:13.131 [Actor Thread 7] DEBUG nextflow.container.SingularityCache - Singularity pull complete image=https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0 path=/workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work/singularity/depot.galaxyproject.org-singularity-fastqc-0.12.1--hdfd78af_0.img +Feb-02 16:20:13.234 [Task submitter] DEBUG n.executor.local.LocalTaskHandler - Launch cmd line: /bin/bash -ue .command.run +Feb-02 16:20:13.238 [Task submitter] INFO nextflow.Session - [46/6e4ea9] Submitted process > FASTQC (test) +Feb-02 16:20:19.641 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[id: 1; name: FASTQC (test); status: COMPLETED; exit: 0; error: -; workDir: /workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work/46/6e4ea98ff77a415eecb6c9ac70079e] +Feb-02 16:20:19.682 [Task monitor] WARN nextflow.processor.PublishDir - Process `FASTQC` publishDir path contains a variable with a null value +Feb-02 16:20:19.685 [Task monitor] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'PublishDir' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:20:19.690 [main] DEBUG nextflow.Session - Session await > all processes finished +Feb-02 16:20:19.695 [Task monitor] DEBUG n.processor.TaskPollingMonitor - <<< barrier arrives (monitor: local) - terminating tasks monitor poll loop +Feb-02 16:20:19.695 [main] DEBUG nextflow.Session - Session await > all barriers passed +Feb-02 16:20:19.700 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'PublishDir' shutdown completed (hard=false) +Feb-02 16:20:19.716 [main] DEBUG n.trace.WorkflowStatsObserver - Workflow completed > WorkflowStats[succeededCount=1; failedCount=0; ignoredCount=0; cachedCount=0; pendingCount=0; submittedCount=0; runningCount=0; retriesCount=0; abortedCount=0; succeedDuration=12s; failedDuration=0ms; cachedDuration=0ms;loadCpus=0; loadMemory=0; peakRunning=1; peakCpus=2; peakMemory=3 GB; ] +Feb-02 16:20:19.717 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow completed -- saving trace file +Feb-02 16:20:19.778 [main] DEBUG nextflow.cache.CacheDB - Closing CacheDB done +Feb-02 16:20:19.778 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-amazon@2.1.4' +Feb-02 16:20:19.778 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-amazon +Feb-02 16:20:19.778 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-validation@1.1.3' +Feb-02 16:20:19.778 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-validation +Feb-02 16:20:19.796 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'FileTransfer' shutdown completed (hard=false) +Feb-02 16:20:19.796 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'S3TransferManager' shutdown completed (hard=false) +Feb-02 16:20:19.796 [main] DEBUG nextflow.script.ScriptRunner - > Execution complete -- Goodbye diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_0.json b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_0.json new file mode 100644 index 0000000..2e79514 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_0.json @@ -0,0 +1 @@ +{"0":[[{"id":"test","single_end":false},"/workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work/46/6e4ea98ff77a415eecb6c9ac70079e/test_fastqc.html"]]} \ No newline at end of file diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_1.json b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_1.json new file mode 100644 index 0000000..6c6e08f --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_1.json @@ -0,0 +1 @@ +{"1":[[{"id":"test","single_end":false},"/workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work/46/6e4ea98ff77a415eecb6c9ac70079e/test_fastqc.zip"]]} \ No newline at end of file diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_2.json b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_2.json new file mode 100644 index 0000000..8b0d274 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_2.json @@ -0,0 +1 @@ +{"2":["/workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work/46/6e4ea98ff77a415eecb6c9ac70079e/versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_html.json b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_html.json new file mode 100644 index 0000000..c3e0591 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_html.json @@ -0,0 +1 @@ +{"html":[[{"id":"test","single_end":false},"/workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work/46/6e4ea98ff77a415eecb6c9ac70079e/test_fastqc.html"]]} \ No newline at end of file diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_versions.json b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_versions.json new file mode 100644 index 0000000..66e0e45 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_versions.json @@ -0,0 +1 @@ +{"versions":["/workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work/46/6e4ea98ff77a415eecb6c9ac70079e/versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_zip.json b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_zip.json new file mode 100644 index 0000000..b5fe5b8 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/output_zip.json @@ -0,0 +1 @@ +{"zip":[[{"id":"test","single_end":false},"/workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/work/46/6e4ea98ff77a415eecb6c9ac70079e/test_fastqc.zip"]]} \ No newline at end of file diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/params.json b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/params.json new file mode 100644 index 0000000..a5db512 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/params.json @@ -0,0 +1 @@ +{"nf_test_output":"/workspaces/readsimulator/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta"} \ No newline at end of file diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/std.err b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/std.err new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/std.out b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/std.out new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/trace.csv b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/trace.csv new file mode 100644 index 0000000..cf2efe2 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/trace.csv @@ -0,0 +1,2 @@ +task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar +1 46/6e4ea9 17735 FASTQC (test) COMPLETED 0 2024-02-02 16:20:13.238 6.4s 6s 101.9% 178.4 MB 3.8 GB 11.2 MB 1.8 MB diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/workflow.json b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/workflow.json new file mode 100644 index 0000000..7d54df0 --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/meta/workflow.json @@ -0,0 +1 @@ +{"success":true,"exitStatus":0} \ No newline at end of file diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/null/fastqc/test_fastqc.html b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/null/fastqc/test_fastqc.html new file mode 100644 index 0000000..5bb165d --- /dev/null +++ b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/null/fastqc/test_fastqc.html @@ -0,0 +1,187 @@ +test.bam FastQC Report
    FastQCFastQC Report
    Fri 2 Feb 2024
    test.bam

    Summary

    [OK]Basic Statistics

    MeasureValue
    Filenametest.bam
    File typeConventional base calls
    EncodingSanger / Illumina 1.9
    Total Sequences200
    Total Bases27.6 kbp
    Sequences flagged as poor quality0
    Sequence length53-151
    %GC39

    [OK]Per base sequence quality

    Per base quality graph

    [OK]Per sequence quality scores

    Per Sequence quality graph

    [FAIL]Per base sequence content

    Per base sequence content

    [FAIL]Per sequence GC content

    Per sequence GC content graph

    [OK]Per base N content

    N content graph

    [WARN]Sequence Length Distribution

    Sequence length distribution

    [OK]Sequence Duplication Levels

    Duplication level graph

    [WARN]Overrepresented sequences

    SequenceCountPercentagePossible Source
    CAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGA21.0No Hit
    CAATAGTCCAGTCAACACGCTTAACAAAGCACTCGTGGACAGCTAGACAC21.0No Hit
    GTACAAAAATAGCCTAAGAAACAATAAACTAGCATTATACACTGAAGTGT21.0No Hit
    ACTCTAGATAATTCATTAGGTTTCTTAATAGTAAGACTAGAATTGTCTAC10.5No Hit
    CCCATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCC10.5No Hit
    TTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTA10.5No Hit
    ATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTA10.5No Hit
    GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT10.5No Hit
    CCAAAAATCCAGCCTCTTATTATGTTAGACTTCTCAGTGGAAGCAAAATA10.5No Hit
    TTGTCATCAGTGCAAGCAGTTTGTGTAGTACCGGCAGCACAAGACATCTG10.5No Hit
    CCTTAATGGTTCATGTGGTAGTGTTGGTTTTAACATAGATTATGACTGTG10.5No Hit
    GTGTTCCCTTGAACATAATACCTCTTACAACAGCAGCCAAACTAATGGTT10.5No Hit
    CATAGTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCATAATTT10.5No Hit
    ACATACTGTTCTAATGTTGAAGTCACTTTGAATTTATCAAAACACTCTAC10.5No Hit
    CTTTATGACAAGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGT10.5No Hit
    TATCTGCCCATTTAATAGAAGTTAAACCATTAACTTGTGGGTATTTCCAC10.5No Hit
    CCCTTGGAGAGTGCTAGTTGCCATCTCTTTTTGAGGGTTATGATTTTGGA10.5No Hit
    GTTTACAACAGTAGACAACATTAACCTCCACACGCAAGTTGTGGACATGT10.5No Hit
    ACTGAATTTTCTGCACCAAGTGACATAGTGTAGGCAATGATGGATTGACT10.5No Hit
    TTAGTAGCCGTATTTAAGGCTCCTGCAACACCTCCTCCATGTTTAAGGTA10.5No Hit
    CTGTGGATCACGGACAGCATCAGTAGTGTCAGCAATGTCTCTGCCAAATT10.5No Hit
    TTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTAATGGGTAA10.5No Hit
    GGTGTATTCAAGGCTCCCTCAGTTGCAACCCATATGATGCCGTCTTTGTT10.5No Hit
    AAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTT10.5No Hit
    CCGCTAACAGTGCAGAAGTGTATTGAGCAATCATTTCATCTGTGAGCAAA10.5No Hit
    AGATAATAGATGGTAGAATGTAAAAGGCACTTTTACACTTTTTAAGCACT10.5No Hit
    ACATAGTGTAGGCAATGATGGATTGACTAGCTACACTACGTGCCCGCCGA10.5No Hit
    ATTAACTATTAACGTACCTGTCTCTTCCGAAACGAATGAGTACATAAGTT10.5No Hit
    GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT10.5No Hit
    CAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCT10.5No Hit
    GATGAAACATCATGCGTGATAACACCCTTATAAAACATTTTAAAGCATTG10.5No Hit
    CACGATGGCAGACCTCGTCTATGCTTTAAGGCATTTTGATGAAGGTAATT10.5No Hit
    GCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGT10.5No Hit
    CAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCT10.5No Hit
    GGTCAAGGTTAATATAGGCATTAACAATGAATAATAAGAATCTACAACAG10.5No Hit
    CTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTG10.5No Hit
    TGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTG10.5No Hit
    ATGTCAGATTTATGTGACAATTTAAGACATTCAACAACATCTTTAGTTTC10.5No Hit
    GGATTAGTAACACTACAGCTGATGCATACATAACACAGTCTTTTAGCTTA10.5No Hit
    TCTATAGGTCATGTCCTTAGGTATGCCAGGTATGTCAACACATAAACCTT10.5No Hit
    TGGTAAGAGAATTCCTTACACGTAACCCTGCTTGGAGAAAAGCTGTCTTT10.5No Hit
    CACAATTAGTGATTGGTTGTCCCCCACTAGCTAGATAATCTTTGTAAGCT10.5No Hit
    CATTGGTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTC10.5No Hit
    GGTTAAAGTCATTAAGAGTTGTGGTAAATCGATTGAGAAACCACCTGTCT10.5No Hit
    TGGCAATAGAGTTATTAGAGTAAGCAACTGAATTTTCTGCACCAAGTGAC10.5No Hit
    TGTTTATAGTGACCACACTGGTAATTACCAGTGTACTCACTAGCACAAGT10.5No Hit
    GTAAAGTACAAATAGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGAT10.5No Hit
    CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGA10.5No Hit
    GATTTTTAAGTGTGTCACTTAACATTTGTACAATCTTTATACGCACTACA10.5No Hit
    CACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATA10.5No Hit
    GCATGAAGCTTTACCAGCACGTGCTAGAAGGTCTTTAATGCACTCAAGAG10.5No Hit
    AGCTACATGTGCATTACCATGGACTTGACAATACAGATCATGGTTGCTTT10.5No Hit
    GATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGC10.5No Hit
    CTTTCGTTGCAATATGGCAGTTTTTGTACACAATTAAACCGTGCTTTAAC10.5No Hit
    GTACGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTA10.5No Hit
    GTCCACACCCAAATTATTGAGTATTTTCACCTCTGGTACTGGTTTAATGT10.5No Hit
    ATGAATCTGATGAATACATAGCTACTAATGGACCTCTTAAAGTGCGTGGT10.5No Hit
    ATACTTAGGTGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTACAC10.5No Hit
    ATAAACATGTTAGGGTAACCATTAACTTGATAATTCATTTTAAAACCCAT10.5No Hit
    GATTTAGCCACATTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGA10.5No Hit
    TTTCTGTACAAACAACACCATCCAATTTATAAGTAACTGGTTTTATGGTT10.5No Hit
    GTTGAAAGTAGATCTTCAATAAATGACCTCTTGCTTGGTTTTGATGGATC10.5No Hit
    CGTGTAAGGAATTCTCTTACCACGCCTATTTGTGGCCTGTTAATTGCAGA10.5No Hit
    GTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTC10.5No Hit
    GCTATAACGCAGCCTGTAAAATCATCTGGTAATTTATAATTATAATCAGC10.5No Hit
    AGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTT10.5No Hit
    TTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATT10.5No Hit
    ATCATGACGTTCGTGTTGTTTTAGATTTCATCGAAACGAACAAACAAAAA10.5No Hit
    ATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGAT10.5No Hit
    CCGTCTATGCAATACAAAGTTTCTTTAGAAGTTATATGTTTATAGTGACC10.5No Hit
    GCAATTCATAGCTCTTTTCAGAACGTTCCGTGTACCAAGCAATTTCATGC10.5No Hit
    TTACAGAGCAAGGGCTGGTGAAGCTGCTAACTTTTGTGCACTTATCTTAG10.5No Hit
    AGTAAACTGTTACAAACAACAACAGCAAGTTGCAAACAAAGTGAACACCC10.5No Hit
    GTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAG10.5No Hit
    CTCCAAAGCCACGTACGAGCACGTCGCGAACCTGTAAAACAGGCAAACTG10.5No Hit
    GTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCA10.5No Hit
    TGTACACATAGTGCTTAGCACGTAATCTGGCATTGACAACACTCAAATCA10.5No Hit
    TGTCATAATACGCATCACCCAACTAGCAGGCATATAGACCATATTAAAAT10.5No Hit
    TCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAG10.5No Hit
    TGCGTCTTGGGTCCCCGCTCTCACTCAACATGGCAAGGAAGACCTTAAAT10.5No Hit
    CTAAAAGTGAAGTCAAAATTGTGAGTAACAACCAGTGGTGTGTACCCGTG10.5No Hit
    ATGTAAGGCTTTGTTAAGTCAGTGTCAACATGTGACTCTGCAGTTAAAGC10.5No Hit
    CCTTGAATGAGTCTAATTCAGGTTGCAAAGGATCATAAACTGTGTTGTTG10.5No Hit
    TGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCA10.5No Hit
    ATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGG10.5No Hit
    ATCCTTTAGGATTTGGATGATCTATGTGGCAACGGCAGTACAGACAACAC10.5No Hit
    CTTGGTTAGCATTAATACAGCCACCATCGTAACAATCAAAGTACTTATCA10.5No Hit
    TGCCTGACCAGTACCAGTGTGTGTACACAACATCTTAACACAATTAGTGA10.5No Hit
    CTACACGAGCACGTGCAGGTATAATTCTACTACATTTATCTATAGGCAAA10.5No Hit
    TTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATA10.5No Hit
    ATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACGCACATTGCTA10.5No Hit
    GTCTAATCATAATTTCTTGGTACAGGCTGGTATTGTTCATCTCAGGGTTA10.5No Hit
    TGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTA10.5No Hit
    TTTCAAGACTTGTAAATTGCAACTTGTCATAAAGGTCTCTATCAGACATT10.5No Hit
    TTTATCTAAGTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGC10.5No Hit
    ATTCTGTGCTGGTAGTACATTTATTAGTGATGAAGATGCGAGAGACTTGT10.5No Hit
    CTTAGGTCTTAGGATTGGCTGTATCAACCTTAAGCTTAAGTACACAATTT10.5No Hit
    AAGTGTATCTTGATCCTCATAACTCATTGAATCATAATAAAGTCTAGCCT10.5No Hit
    GTACACACACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAAT10.5No Hit
    ATTATACTTAAACCAGCAAATAATAGTTTAAAAATTACAGAAGAGGTTGG10.5No Hit
    GTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTC10.5No Hit
    AATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTA10.5No Hit
    GCTAAGCACTATGTGTACATTGGCGACCCTGCTCAATTACCTGCACCACG10.5No Hit
    CAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAAT10.5No Hit
    TCCCTGCACAAGAAAAGAACTTCACAACTGCTCCTGCCATTTGTCATGAT10.5No Hit
    GTTCCTAGGTAGTAGAAATACCATCTAGGACTGAGATCTTTCATTTTACA10.5No Hit
    CATCTTATTTCTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGA10.5No Hit
    CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT10.5No Hit
    CCTTTTGATGTTGTTAGACAATGCTCAGGTGTTACTTTCCAAAGTGCAGT10.5No Hit
    GGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCAT10.5No Hit
    ATATAATTCCGCATCATTTTCCACTTTTAAGTGTTATGGAGTGTCTCCTA10.5No Hit
    AACTAGCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGC10.5No Hit
    GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG10.5No Hit
    AAGTTGGCGTATACGCGTAATATATCTGGGTTTTCTACAAAATCATACCA10.5No Hit
    TTCCAGAAGAGCAAGGTTCTTTTAAAAGTACTGTTGTACCTCTAACACAC10.5No Hit
    TATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAA10.5No Hit
    TGATCAACCTATCAATTTGCACTTCAGCCTCAACTTTGTCAAGACGTGAA10.5No Hit
    CTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATATGG10.5No Hit
    GGTGGCTGTATTAATGCTAACCAAGTCATCGTCAACAACCTAGACAAATC10.5No Hit
    GTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTA10.5No Hit
    GCGTACAACCAAGCTAAAGTTACTGGCCATAACAGCCAGAGGAAAATTAA10.5No Hit
    CTCAGTTGCAACCCATATGATGCCGTCTTTGTTAGCACCATAGGGAAGTC10.5No Hit
    TCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTAT10.5No Hit
    GGCACCTTCTTTAGTCAAATTCTCAGTGCCACAAAATTCGCAAGTGGCTT10.5No Hit
    GTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAA10.5No Hit
    CAATTTTACTATCTGCATCTACAACCTGTTGGATTTCCCACAATGCTGAT10.5No Hit
    GGATAGACAGATCGAATTCTACCCATAAAGCCATCAAGCTTTTTCTTTTC10.5No Hit
    GGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTATCTTTCTGTGC10.5No Hit
    ACTGTTTTCTTTGTAGAAAACATCCGTAATAGGACCTTTGTATTCTGAGG10.5No Hit
    GTTATAACACTGACACCACCAAAAGAACATGGTGTAATGTCAAGAATCTC10.5No Hit
    GTGCATTACCATGGACTTGACAATACAGATCATGGTTGCTTTGTAGGTTA10.5No Hit
    GTTGTAGTAAGCTAACGCATTGTCATCAGTGCAAGCAGTTTGTGTAGTAC10.5No Hit
    CCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGAC10.5No Hit
    GCATTTCTTCACAAAGCTTGTTTATGTCTACAGCACCCTGCATGGAAAGC10.5No Hit
    GTTACTCACAATTTTGACTTCACTTTTAGTCTTAGTCCAGAGTACTCAAT10.5No Hit
    CATTAGCACAAGTTGTAGGTATTTGTACATACTTACCTTTTAAGTCACAA10.5No Hit
    TGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTTGT10.5No Hit
    TCATAGATGTCAACTCAAAGCCATGTGCCCATAAGACAAATACGACTCTG10.5No Hit
    ATAACACCCTTATAAAACATTTTAAAGCATTGAGCTGATTTGTCTTTATG10.5No Hit
    TCTTTGTAAGCTTTAGCAGCATCTACAGCAAAAGCACAGAAAGATAATAC10.5No Hit
    CTTTGAAGCAAGGTGAAATCAAGGATGCTACTCCTTCAGATTTTCTTCGC10.5No Hit
    CTAAAAAGGCTGGTGGCACTACTGAAATGCTAGCGAAAGCTTTGAGAAAA10.5No Hit
    CTGTAATAGTTGTGTCCGTACCAGCTGCTTGTGCTGTTTGCCTGTCAACA10.5No Hit
    GAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTG10.5No Hit
    TGGTAATGCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTG10.5No Hit
    CTTTTACAAGACTTCAGAGTTTAGAAATTGTGGCTTATAATGTTGTAATT10.5No Hit
    CAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTGGATGATTTCTCCAA10.5No Hit
    CAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCAT10.5No Hit
    GCTTTAGATCAAGCCATTTCCATGTGGGCTCTTATAATCTCTGTTACTTC10.5No Hit
    CACAAACTCTAAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCAGAT10.5No Hit
    AACACCAGCTTCTGATCTTTCACAAGTGCCGTGCCTACAGTACTCAGAAT10.5No Hit
    AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC10.5No Hit
    AGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTA10.5No Hit
    CATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTT10.5No Hit
    ACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAA10.5No Hit
    GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG10.5No Hit
    TGCCAATCCTGTAGCGACTGTATGCAGCAAAACCTGAGTCACCTGCTACA10.5No Hit
    GTACTGGTAAGAGTCATTTTGCTATTGGCCTAGCTCTCTACTACCCTTCT10.5No Hit
    TCTTTGTTAGCACCATAGGGAAGTCCAGATTCTGGCCCAGGTCCTAGGTA10.5No Hit
    ATATTGCAGCAGTACGCACACAATCGAAGCGCAGTAAGGATGGCTAGTGT10.5No Hit
    CTATAACATTAATAGGCAATGAACCTTTAGTGTTATTAGCTCTCAGGTTG10.5No Hit
    ACTCATATGAGTGTGACATACCCATTGGTGCAGGTATATGCGCTAGTTAT10.5No Hit
    GCATAGACGGTGCTTTACTTACAAAGTCCTCAGAATACAAAGGTCCTATT10.5No Hit
    CCAAGCAGGGTTACGTGTAAGGAATTCTCTTACCACGCCTATTTGTGGCC10.5No Hit
    AGCTAACACTGAAAAAGTCTGTCCTGGTTGAATGCGAACAAACTTATACT10.5No Hit
    TATAATTAATAACTAATTACTGTCGTTGACAGGACACGAGTAACTCGTCT10.5No Hit
    TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCG10.5No Hit
    TAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCG10.5No Hit
    CCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAATTTCAACTT10.5No Hit
    TCTAAGCATAGTGAAAAGCATTGTCTGCATAGCACTAGTAACTTTTGCCC10.5No Hit
    GATCAGGCATTAGTGTCTGATGTTGGTGATAGTGCGGAAGTTGCAGTTAA10.5No Hit
    ATCATACACAGTTCTTGCTGTCATAAGGATTAGTAACACTACAGCTGATG10.5No Hit
    GAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGA10.5No Hit
    GTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTG10.5No Hit
    GTTGTAAACACCTTAATAGTCCTCACTTCTCTCAAAGAAAGAAGTGTCTT10.5No Hit
    CATGGATTGGCTTCGATGTCGAGGGGTGTCATGCTACTAGAGAAGCTGTT10.5No Hit
    GTACTATCTATACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACA10.5No Hit
    ATACTGCGTCAAGGTTCACCGCACTCACTCAACATGCCAAGGAAGACATA10.5No Hit
    ATTATAGGATATTCAATAGTCCAGTCAACACGCTTAACAAAGCACTCGTG10.5No Hit
    TGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACA10.5No Hit
    GTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAATTGTCTGT10.5No Hit
    GATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACA10.5No Hit
    ATCTAAAGTCATTTGACTTAGGCCACGAGCTTGGCACTGATCCTTATGAA10.5No Hit
    GTGAAATTAGTATGGACAAATCACCTAATTTAGCATGGCCTCTTATTGTA10.5No Hit
    GTACACGGAACGTTCTGAAAAGAGCTATGAATTGCAGACACCTTTTGAAA10.5No Hit
    GCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGC10.5No Hit
    AACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCA10.5No Hit
    GCATTTCTTACCTAGAGTTTTTAGTGCAGTTGGTAACATCTGTTACACAC10.5No Hit
    GTTAAAAACAGTACAATTCTGTGATGCCATGCGAAATGCTGGTATTGTTG10.5No Hit
    GCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGC10.5No Hit
    GCTGCTAGAGACCTCGTTTGTGCACAAAAGTTTAACGGCCTTACTGTTTT10.5No Hit
    CCCTTTGTTGTGTTGTAGTAAGCTAACGCATTGTCATCAGTGCAAGCAGT10.5No Hit
    ATAGACAACAGGTGCGCTCAGGTCCTATTTTCACAAAATACTTCATAGAT10.5No Hit
    GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT10.5No Hit
    CATTTGGTGACGCAACTGGATAGACAGATCGAATTCTACCCATAAAGCCA10.5No Hit
    ATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTT10.5No Hit
    ATCAAGGACCTGCCTAAAGAAATCACTGTTGCTACATCACGAACGCTTTC10.5No Hit

    [OK]Adapter Content

    Adapter graph

    Produced by FastQC (version 0.12.1)
    \ No newline at end of file diff --git a/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/null/fastqc/test_fastqc.zip b/.nf-test/tests/3cdc93c0cf87b24597bf6efe9c16b1bd/null/fastqc/test_fastqc.zip new file mode 100644 index 0000000000000000000000000000000000000000..626f3e42c547342b7a19771742b3797c6f9c9313 GIT binary patch literal 380763 zcmce-1CT9Ew=LSXZQHhYyLa2Wt=+b5+qP}nwr$()e*OLD+!y!!@12Nu?z})>iiaOCFH_-Gil`vC;pU3&MYQ@vk8MBO4vlp94A@D`UV)5QX

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"1.2", + "body": [ + { + "type": "TextBlock", + "size": "Large", + "weight": "Bolder", + "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", + "text": "nf-core/readsimulator v${version} - ${runName}", + "wrap": true + }, + { + "type": "TextBlock", + "spacing": "None", + "text": "Completed at ${dateComplete} (duration: ${duration})", + "isSubtle": true, + "wrap": true + }, + { + "type": "TextBlock", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors. The full error message was: ${errorReport}.<% } %>", + "wrap": true + }, + { + "type": "TextBlock", + "text": "The command used to launch the workflow was as follows:", + "wrap": true + }, + { + "type": "TextBlock", + "text": "${commandLine}", + "isSubtle": true, + "wrap": true + } + ], + "actions": [ + { + "type": "Action.ShowCard", + "title": "Pipeline Configuration", + "card": { + "type": "AdaptiveCard", + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "body": [ + { + "type": "FactSet", + "facts": [<% out << summary.collect{ k,v -> "{\"title\": \"$k\", \"value\" : \"$v\"}"}.join(",\n") %> + ] + } + ] + } + } + ] + } + } + ] +} diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/email_template.html b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/email_template.html new file mode 100644 index 0000000..f2bc049 --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/email_template.html @@ -0,0 +1,53 @@ + + + + + + + + nf-core/readsimulator Pipeline Report + + +

     + + + diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/email_template.txt b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/email_template.txt new file mode 100644 index 0000000..6ca5507 --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/email_template.txt @@ -0,0 +1,39 @@ +---------------------------------------------------- + ,--./,-. + ___ __ __ __ ___ /,-._.--~\\ + |\\ | |__ __ / ` / \\ |__) |__ } { + | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, + `._,._,' + nf-core/readsimulator ${version} +---------------------------------------------------- +Run Name: $runName + +<% if (success){ + out << "## nf-core/readsimulator execution completed successfully! ##" +} else { + out << """#################################################### +## nf-core/readsimulator execution completed unsuccessfully! ## +#################################################### +The exit status of the task that caused the workflow execution to fail was: $exitStatus. +The full error message was: + +${errorReport} +""" +} %> + + +The workflow was completed at $dateComplete (duration: $duration) + +The command used to launch the workflow was as follows: + + $commandLine + + + +Pipeline Configuration: +----------------------- +<% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> + +-- +nf-core/readsimulator +https://github.com/nf-core/readsimulator diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/methods_description_template.yml b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/methods_description_template.yml new file mode 100644 index 0000000..6119677 --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/methods_description_template.yml @@ -0,0 +1,29 @@ +id: "nf-core-readsimulator-methods-description" +description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." +section_name: "nf-core/readsimulator Methods Description" +section_href: "https://github.com/nf-core/readsimulator" +plot_type: "html" +## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline +## You inject any metadata in the Nextflow '${workflow}' object +data: | +

    Methods

    +

    Data was processed using nf-core/readsimulator v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

    +

    The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

    + 
    ${workflow.commandLine}
    +

    ${tool_citations}

    +

    References

    +
      +
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
      • +
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
      • +
    • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
      • +
    • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
      • + ${tool_bibliography} +
    +
    +
    Notes:
    +
      + ${nodoi_text} +
    • The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
      • +
    • You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.
      • +
    +
    diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/multiqc_config.yml b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/multiqc_config.yml new file mode 100644 index 0000000..7d3650d --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/multiqc_config.yml @@ -0,0 +1,15 @@ +report_comment: > + + This report has been generated by the nf-core/readsimulator + analysis pipeline. 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charset=utf-8 + +$email_html + +--nfcoremimeboundary +Content-Type: image/png;name="nf-core-readsimulator_logo.png" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: inline; filename="nf-core-readsimulator_logo_light.png" + +<% out << new File("$projectDir/assets/nf-core-readsimulator_logo_light.png"). + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' ) %> + +<% +if (mqcFile){ +def mqcFileObj = new File("$mqcFile") +if (mqcFileObj.length() < mqcMaxSize){ +out << """ +--nfcoremimeboundary +Content-Type: text/html; name=\"multiqc_report\" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" + +${mqcFileObj. + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} +""" +}} +%> + +--nfcoremimeboundary-- diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/slackreport.json b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/slackreport.json new file mode 100644 index 0000000..e9d5f8a --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/assets/slackreport.json @@ -0,0 +1,34 @@ +{ + "attachments": [ + { + "fallback": "Plain-text summary of the attachment.", + "color": "<% if (success) { %>good<% } else { %>danger<%} %>", + "author_name": "nf-core/readsimulator ${version} - ${runName}", + "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", + "fields": [ + { + "title": "Command used to launch the workflow", + "value": "```${commandLine}```", + "short": false + } + <% + if (!success) { %> + , + { + "title": "Full error message", + "value": "```${errorReport}```", + "short": false + }, + { + "title": "Pipeline configuration", + "value": "<% out << summary.collect{ k,v -> k == "hook_url" ? "_${k}_: (_hidden_)" : ( ( v.class.toString().contains('Path') || ( v.class.toString().contains('String') && v.contains('/') ) ) ? "_${k}_: `${v}`" : (v.class.toString().contains('DateTime') ? ("_${k}_: " + v.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM))) : "_${k}_: ${v}") ) }.join(",\n") %>", + "short": false + } + <% } + %> + ], + "footer": "Completed at <% out << dateComplete.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM)) %> (duration: ${duration})" + } + ] +} diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/bin/check_samplesheet.py b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/bin/check_samplesheet.py new file mode 100755 index 0000000..4a758fe --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/bin/check_samplesheet.py @@ -0,0 +1,259 @@ +#!/usr/bin/env python + + +"""Provide a command line tool to validate and transform tabular samplesheets.""" + + +import argparse +import csv +import logging +import sys +from collections import Counter +from pathlib import Path + +logger = logging.getLogger() + + +class RowChecker: + """ + Define a service that can validate and transform each given row. + + Attributes: + modified (list): A list of dicts, where each dict corresponds to a previously + validated and transformed row. The order of rows is maintained. + + """ + + VALID_FORMATS = ( + ".fq.gz", + ".fastq.gz", + ) + + def __init__( + self, + sample_col="sample", + first_col="fastq_1", + second_col="fastq_2", + single_col="single_end", + **kwargs, + ): + """ + Initialize the row checker with the expected column names. + + Args: + sample_col (str): The name of the column that contains the sample name + (default "sample"). + first_col (str): The name of the column that contains the first (or only) + FASTQ file path (default "fastq_1"). + second_col (str): The name of the column that contains the second (if any) + FASTQ file path (default "fastq_2"). + single_col (str): The name of the new column that will be inserted and + records whether the sample contains single- or paired-end sequencing + reads (default "single_end"). + + """ + super().__init__(**kwargs) + self._sample_col = sample_col + self._first_col = first_col + self._second_col = second_col + self._single_col = single_col + self._seen = set() + self.modified = [] + + def validate_and_transform(self, row): + """ + Perform all validations on the given row and insert the read pairing status. + + Args: + row (dict): A mapping from column headers (keys) to elements of that row + (values). + + """ + self._validate_sample(row) + self._validate_first(row) + self._validate_second(row) + self._validate_pair(row) + self._seen.add((row[self._sample_col], row[self._first_col])) + self.modified.append(row) + + def _validate_sample(self, row): + """Assert that the sample name exists and convert spaces to underscores.""" + if len(row[self._sample_col]) <= 0: + raise AssertionError("Sample input is required.") + # Sanitize samples slightly. + row[self._sample_col] = row[self._sample_col].replace(" ", "_") + + def _validate_first(self, row): + """Assert that the first FASTQ entry is non-empty and has the right format.""" + if len(row[self._first_col]) <= 0: + raise AssertionError("At least the first FASTQ file is required.") + self._validate_fastq_format(row[self._first_col]) + + def _validate_second(self, row): + """Assert that the second FASTQ entry has the right format if it exists.""" + if len(row[self._second_col]) > 0: + self._validate_fastq_format(row[self._second_col]) + + def _validate_pair(self, row): + """Assert that read pairs have the same file extension. Report pair status.""" + if row[self._first_col] and row[self._second_col]: + row[self._single_col] = False + first_col_suffix = Path(row[self._first_col]).suffixes[-2:] + second_col_suffix = Path(row[self._second_col]).suffixes[-2:] + if first_col_suffix != second_col_suffix: + raise AssertionError("FASTQ pairs must have the same file extensions.") + else: + row[self._single_col] = True + + def _validate_fastq_format(self, filename): + """Assert that a given filename has one of the expected FASTQ extensions.""" + if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): + raise AssertionError( + f"The FASTQ file has an unrecognized extension: {filename}\n" + f"It should be one of: {', '.join(self.VALID_FORMATS)}" + ) + + def validate_unique_samples(self): + """ + Assert that the combination of sample name and FASTQ filename is unique. + + In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the + number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. + + """ + if len(self._seen) != len(self.modified): + raise AssertionError("The pair of sample name and FASTQ must be unique.") + seen = Counter() + for row in self.modified: + sample = row[self._sample_col] + seen[sample] += 1 + row[self._sample_col] = f"{sample}_T{seen[sample]}" + + +def read_head(handle, num_lines=10): + """Read the specified number of lines from the current position in the file.""" + lines = [] + for idx, line in enumerate(handle): + if idx == num_lines: + break + lines.append(line) + return "".join(lines) + + +def sniff_format(handle): + """ + Detect the tabular format. + + Args: + handle (text file): A handle to a `text file`_ object. The read position is + expected to be at the beginning (index 0). + + Returns: + csv.Dialect: The detected tabular format. + + .. _text file: + https://docs.python.org/3/glossary.html#term-text-file + + """ + peek = read_head(handle) + handle.seek(0) + sniffer = csv.Sniffer() + dialect = sniffer.sniff(peek) + return dialect + + +def check_samplesheet(file_in, file_out): + """ + Check that the tabular samplesheet has the structure expected by nf-core pipelines. + + Validate the general shape of the table, expected columns, and each row. Also add + an additional column which records whether one or two FASTQ reads were found. + + Args: + file_in (pathlib.Path): The given tabular samplesheet. The format can be either + CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. + file_out (pathlib.Path): Where the validated and transformed samplesheet should + be created; always in CSV format. + + Example: + This function checks that the samplesheet follows the following structure, + see also the `viral recon samplesheet`_:: + + sample,fastq_1,fastq_2 + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + + .. _viral recon samplesheet: + https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + + """ + required_columns = {"sample", "fastq_1", "fastq_2"} + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_in.open(newline="") as in_handle: + reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) + # Validate the existence of the expected header columns. + if not required_columns.issubset(reader.fieldnames): + req_cols = ", ".join(required_columns) + logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") + sys.exit(1) + # Validate each row. + checker = RowChecker() + for i, row in enumerate(reader): + try: + checker.validate_and_transform(row) + except AssertionError as error: + logger.critical(f"{str(error)} On line {i + 2}.") + sys.exit(1) + checker.validate_unique_samples() + header = list(reader.fieldnames) + header.insert(1, "single_end") + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_out.open(mode="w", newline="") as out_handle: + writer = csv.DictWriter(out_handle, header, delimiter=",") + writer.writeheader() + for row in checker.modified: + writer.writerow(row) + + +def parse_args(argv=None): + """Define and immediately parse command line arguments.""" + parser = argparse.ArgumentParser( + description="Validate and transform a tabular samplesheet.", + epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", + ) + parser.add_argument( + "file_in", + metavar="FILE_IN", + type=Path, + help="Tabular input samplesheet in CSV or TSV format.", + ) + parser.add_argument( + "file_out", + metavar="FILE_OUT", + type=Path, + help="Transformed output samplesheet in CSV format.", + ) + parser.add_argument( + "-l", + "--log-level", + help="The desired log level (default WARNING).", + choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), + default="WARNING", + ) + return parser.parse_args(argv) + + +def main(argv=None): + """Coordinate argument parsing and program execution.""" + args = parse_args(argv) + logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") + if not args.file_in.is_file(): + logger.error(f"The given input file {args.file_in} was not found!") + sys.exit(2) + args.file_out.parent.mkdir(parents=True, exist_ok=True) + check_samplesheet(args.file_in, args.file_out) + + +if __name__ == "__main__": + sys.exit(main()) diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/NfcoreTemplate.groovy b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/NfcoreTemplate.groovy new file mode 100755 index 0000000..e248e4c --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/NfcoreTemplate.groovy @@ -0,0 +1,356 @@ +// +// This file holds several functions used within the nf-core pipeline template. +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +class NfcoreTemplate { + + // + // Check AWS Batch related parameters have been specified correctly + // + public static void awsBatch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + // Check params.awsqueue and params.awsregion have been set if running on AWSBatch + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + } + } + + // + // Warn if a -profile or Nextflow config has not been provided to run the pipeline + // + public static void checkConfigProvided(workflow, log) { + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + } + } + + // + // Generate version string + // + public static String version(workflow) { + String version_string = "" + + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string + } + + // + // Construct and send completion email + // + public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = NfcoreTemplate.version(workflow) + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() + } + + // + // Construct and send a notification to a web server as JSON + // e.g. Microsoft Teams and Slack + // + public static void IM_notification(workflow, params, summary_params, projectDir, log) { + def hook_url = params.hook_url + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = NfcoreTemplate.version(workflow) + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("$projectDir/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } + } + + // + // Dump pipeline parameters in a json file + // + public static void dump_parameters(workflow, params) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() + } + + // + // Print pipeline summary on completion + // + public static void summary(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } + + // + // ANSII Colours used for terminal logging + // + public static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + // + // Does what is says on the tin + // + public static String dashedLine(monochrome_logs) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + // + // nf-core logo + // + public static String logo(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String workflow_version = NfcoreTemplate.version(workflow) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/NftestUtils.groovy b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/NftestUtils.groovy new file mode 100644 index 0000000..ce6fc8b --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/NftestUtils.groovy @@ -0,0 +1,38 @@ +// Helper functions for pipeline tests + +class NftestUtils { + + // Function to remove Nextflow version from software_versions.yml + public static String removeNextflowVersion(outputDir) { + def softwareVersions = path("$outputDir/pipeline_info/software_versions.yml").yaml + if (softwareVersions.containsKey("Workflow")) { + softwareVersions.Workflow.remove("Nextflow") + } + return softwareVersions + } + + // Function to filter lines from a file and return a new file + public static File filterLines(String inFilePath, int linesToSkip) { + if (linesToSkip >= 0) { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lineCount = 0 + inputFile.eachLine { line -> + lineCount++ + if (lineCount > linesToSkip) { + outputFile.append(line + '\n') + } + } + return outputFile + } else { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lines = inputFile.readLines() + def totalLines = lines.size() + lines.take(totalLines + linesToSkip).each { line -> + outputFile.append(line + '\n') + } + return outputFile + } + } +} diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/Utils.groovy b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/Utils.groovy new file mode 100644 index 0000000..8d030f4 --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/Utils.groovy @@ -0,0 +1,47 @@ +// +// This file holds several Groovy functions that could be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml + +class Utils { + + // + // When running with -profile conda, warn if channels have not been set-up appropriately + // + public static void checkCondaChannels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } + } +} diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/WorkflowMain.groovy b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/WorkflowMain.groovy new file mode 100755 index 0000000..477ddaf --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/WorkflowMain.groovy @@ -0,0 +1,77 @@ +// +// This file holds several functions specific to the main.nf workflow in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow + +class WorkflowMain { + + // + // Citation string for pipeline + // + public static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + // TODO nf-core: Add Zenodo DOI for pipeline after first release + //"* The pipeline\n" + + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + + // + // Validate parameters and print summary to screen + // + public static void initialise(workflow, params, log, args) { + + // Print workflow version and exit on --version + if (params.version) { + String workflow_version = NfcoreTemplate.version(workflow) + log.info "${workflow.manifest.name} ${workflow_version}" + System.exit(0) + } + + // Check that a -profile or Nextflow config has been provided to run the pipeline + NfcoreTemplate.checkConfigProvided(workflow, log) + // Check that the profile doesn't contain spaces and doesn't end with a trailing comma + checkProfile(workflow.profile, args, log) + + // Check that conda channels are set-up correctly + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + Utils.checkCondaChannels(log) + } + + // Check AWS batch settings + NfcoreTemplate.awsBatch(workflow, params) + + // Check input has been provided + if (!params.input) { + Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") + } + } + // + // Get attribute from genome config file e.g. fasta + // + public static Object getGenomeAttribute(params, attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null + } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } +} diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/WorkflowReadsimulator.groovy b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/WorkflowReadsimulator.groovy new file mode 100755 index 0000000..c0b9df0 --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib/WorkflowReadsimulator.groovy @@ -0,0 +1,131 @@ +// +// This file holds several functions specific to the workflow/readsimulator.nf in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow +import groovy.text.SimpleTemplateEngine + +class WorkflowReadsimulator { + + // + // Check and validate parameters + // + public static void initialise(params, log) { + + genomeExistsError(params, log) + + if (!params.fasta && !params.ncbidownload_accessions && !params.ncbidownload_taxids) { + Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file. Alternatively, '--ncbidownload_accessions accessions.txt' or '--ncbidownload_taxids taxids.txt' can be used." + } + } + + // + // Get workflow summary for MultiQC + // + public static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } + + // + // Generate methods description for MultiQC + // + + public static String toolCitationText(params) { + def citation_text = [ + "Tools used in the workflow included:", + params["amplicon"] ? [ + "CRABS (Jeunen et al. 2022),", + "ART (Huang et al. 2012)," + ].join(' ').trim() : "", + params["target_capture"] ? [ + "Bowtie2 (Langmead et al. 2012),", + "Samtools (Danecek et al. 2021),", + "CapSim (Cao et al. 2018)," + ].join(' ').trim() : "", + params["metagenome"] ? + "InSilicoSeq (Gourlé et al. 2018)," : "", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text + } + + public static String toolBibliographyText(params) { + def reference_text = [ + params["amplicon"] ? [ + "
  • Jeunen, G.-J., Dowle, E., Edgecombe, J., von Ammon, U., Gemmell, N. J., & Cross, H. (2022). crabs—A software program to generate curated reference databases for metabarcoding sequencing data. Molecular Ecology Resources, 00, 1– 14. https://doi.org/10.1111/1755-0998.13741
    • ", + "
  • Weichun Huang, Leping Li, Jason R. Myers, Gabor T. Marth, ART: a next-generation sequencing read simulator, Bioinformatics, Volume 28, Issue 4, February 2012, Pages 593–594, https://doi.org/10.1093/bioinformatics/btr708
    • " + ].join(' ').trim() : "", + params["target_capture"] ? [ + "
  • Langmead, B., Salzberg, S. Fast gapped-read alignment with Bowtie 2. Nat Methods 9, 357–359 (2012). https://doi.org/10.1038/nmeth.1923
    • ", + "
  • Twelve years of SAMtools and BCFtools. Petr Danecek, James K Bonfield, Jennifer Liddle, John Marshall, Valeriu Ohan, Martin O Pollard, Andrew Whitwham, Thomas Keane, Shane A McCarthy, Robert M Davies, Heng Li. GigaScience, Volume 10, Issue 2, February 2021, giab008, https://doi.org/10.1093/gigascience/giab008
    • ", + "
  • Minh Duc Cao, Devika Ganesamoorthy, Chenxi Zhou, Lachlan J M Coin, Simulating the dynamics of targeted capture sequencing with CapSim, Bioinformatics, Volume 34, Issue 5, March 2018, Pages 873–874, https://doi.org/10.1093/bioinformatics/btx691
    • " + ].join(' ').trim() : "", + params["metagenome"] ? + "
  • Gourlé H, Karlsson-Lindsjö O, Hayer J and Bongcam+Rudloff E, Simulating Illumina data with InSilicoSeq. Bioinformatics (2018) doi:10.1093/bioinformatics/bty630
    • " : "", + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text + } + + public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = run_workflow.toMap() + meta["manifest_map"] = run_workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + meta["tool_bibliography"] = toolBibliographyText(params) + + + def methods_text = mqc_methods_yaml.text + + def engine = new SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html + } + + // + // Exit pipeline if incorrect --genome key provided + // + private static void genomeExistsError(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + Nextflow.error(error_string) + } + } +} diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/mock.nf b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/mock.nf new file mode 100644 index 0000000..d075e2d --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/mock.nf @@ -0,0 +1,85 @@ +import groovy.json.JsonGenerator +import groovy.json.JsonGenerator.Converter + +nextflow.enable.dsl=2 + +// comes from nf-test to store json files +params.nf_test_output = "" + +// include dependencies + + +// include test process +include { FASTQC } from '/workspaces/readsimulator/./modules/nf-core/fastqc/tests/../main.nf' + +// define custom rules for JSON that will be generated. +def jsonOutput = + new JsonGenerator.Options() + .excludeNulls() // Do not include fields with value null.. + .addConverter(Path) { value -> value.toString() } // Custom converter for Path. Only filename + .build() + + +workflow { + + // run dependencies + + + // process mapping + def input = [] + + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + + //---- + + //run process + FASTQC(*input) + + if (FASTQC.output){ + + // consumes all named output channels and stores items in a json file + for (def name in FASTQC.out.getNames()) { + serializeChannel(name, FASTQC.out.getProperty(name), jsonOutput) + } + + // consumes all unnamed output channels and stores items in a json file + def array = FASTQC.out as Object[] + for (def i = 0; i < array.length ; i++) { + serializeChannel(i, array[i], jsonOutput) + } + + } + +} + +def serializeChannel(name, channel, jsonOutput) { + def _name = name + def list = [ ] + channel.subscribe( + onNext: { + list.add(it) + }, + onComplete: { + def map = new HashMap() + map[_name] = list + def filename = "${params.nf_test_output}/output_${_name}.json" + new File(filename).text = jsonOutput.toJson(map) + } + ) +} + + +workflow.onComplete { + + def result = [ + success: workflow.success, + exitStatus: workflow.exitStatus, + errorMessage: workflow.errorMessage, + errorReport: workflow.errorReport + ] + new File("${params.nf_test_output}/workflow.json").text = jsonOutput.toJson(result) + +} diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/nextflow.log b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/nextflow.log new file mode 100644 index 0000000..249491c --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/nextflow.log @@ -0,0 +1,100 @@ +Feb-02 16:20:44.200 [main] DEBUG nextflow.cli.Launcher - $> nextflow -quiet -log /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/nextflow.log run /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/mock.nf -c /workspaces/readsimulator/nextflow.config -c /workspaces/readsimulator/tests/nextflow.config -params-file /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/params.json -ansi-log false -profile singularity -with-trace /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/trace.csv -w /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work +Feb-02 16:20:44.306 [main] INFO nextflow.cli.CmdRun - N E X T F L O W ~ version 23.10.1 +Feb-02 16:20:44.330 [main] DEBUG nextflow.plugin.PluginsFacade - Setting up plugin manager > mode=prod; embedded=false; plugins-dir=/home/gitpod/.nextflow/plugins; core-plugins: nf-amazon@2.1.4,nf-azure@1.3.3,nf-cloudcache@0.3.0,nf-codecommit@0.1.5,nf-console@1.0.6,nf-ga4gh@1.1.0,nf-google@1.8.3,nf-tower@1.6.3,nf-wave@1.0.1 +Feb-02 16:20:44.345 [main] INFO o.pf4j.DefaultPluginStatusProvider - Enabled plugins: [] +Feb-02 16:20:44.347 [main] INFO o.pf4j.DefaultPluginStatusProvider - Disabled plugins: [] +Feb-02 16:20:44.352 [main] INFO org.pf4j.DefaultPluginManager - PF4J version 3.4.1 in 'deployment' mode +Feb-02 16:20:44.365 [main] INFO org.pf4j.AbstractPluginManager - No plugins +Feb-02 16:20:44.392 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /workspaces/readsimulator/nextflow.config +Feb-02 16:20:44.393 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /workspaces/readsimulator/tests/nextflow.config +Feb-02 16:20:44.394 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /workspaces/readsimulator/nextflow.config +Feb-02 16:20:44.394 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /workspaces/readsimulator/tests/nextflow.config +Feb-02 16:20:44.433 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity` +Feb-02 16:20:47.437 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity` +Feb-02 16:20:48.421 [main] DEBUG nextflow.config.ConfigBuilder - Available config profiles: [cfc_dev, uzl_omics, ifb_core, denbi_qbic, alice, mjolnir_globe, uppmax, incliva, ilifu, uge, rosalind_uge, lugh, unibe_ibu, vai, czbiohub_aws, jax, ccga_med, scw, tigem, tubingen_apg, google, apollo, ipop_up, pdc_kth, googlels, daisybio, eddie, medair, biowulf, apptainer, bi, bigpurple, adcra, cedars, pawsey_setonix, vsc_kul_uhasselt, pawsey_nimbus, ucl_myriad, utd_ganymede, charliecloud, icr_davros, ceres, munin, arm, rosalind, hasta, cfc, uzh, ebi_codon_slurm, ebc, ku_sund_dangpu, ccga_dx, crick, marvin, biohpc_gen, shifter, mana, mamba, unc_lccc, wehi, awsbatch, wustl_htcf, imperial, maestro, software_license, genotoul, nci_gadi, abims, janelia, nu_genomics, googlebatch, oist, sahmri, mpcdf, leicester, vsc_ugent, create, sage, cambridge, jex, podman, ebi_codon, cheaha, xanadu, nyu_hpc, test, computerome, ucd_sonic, seg_globe, sanger, dkfz, pasteur, ethz_euler, test_full, imb, tuos_stanage, azurebatch, hki, crukmi, csiro_petrichor, docker, engaging, gis, hypatia, psmn, eva, nygc, fgcz, conda, crg, singularity, self_hosted_runner, uw_hyak_pedslabs, utd_sysbio, debug, genouest, cbe, phoenix, gitpod, seawulf, uod_hpc, fub_curta, uct_hpc, aws_tower, binac] +Feb-02 16:20:48.531 [main] DEBUG nextflow.cli.CmdRun - Applied DSL=2 from script declararion +Feb-02 16:20:48.555 [main] INFO nextflow.cli.CmdRun - Launching `/workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/mock.nf` [ecstatic_cori] DSL2 - revision: 60ba27e60b +Feb-02 16:20:48.556 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins declared=[nf-validation@1.1.3] +Feb-02 16:20:48.556 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins default=[] +Feb-02 16:20:48.557 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins resolved requirement=[nf-validation@1.1.3] +Feb-02 16:20:48.557 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-validation version: 1.1.3 +Feb-02 16:20:48.567 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-validation@1.1.3' resolved +Feb-02 16:20:48.568 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-validation@1.1.3' +Feb-02 16:20:48.588 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-validation@1.1.3 +Feb-02 16:20:48.601 [main] DEBUG n.secret.LocalSecretsProvider - Secrets store: /home/gitpod/.nextflow/secrets/store.json +Feb-02 16:20:48.606 [main] DEBUG nextflow.secret.SecretsLoader - Discovered secrets providers: [nextflow.secret.LocalSecretsProvider@2ee92e7d] - activable => nextflow.secret.LocalSecretsProvider@2ee92e7d +Feb-02 16:20:48.746 [main] DEBUG nextflow.Session - Session UUID: d43840f8-afdc-4db7-8003-016db54c90d9 +Feb-02 16:20:48.746 [main] DEBUG nextflow.Session - Run name: ecstatic_cori +Feb-02 16:20:48.747 [main] DEBUG nextflow.Session - Executor pool size: 8 +Feb-02 16:20:48.757 [main] DEBUG nextflow.file.FilePorter - File porter settings maxRetries=3; maxTransfers=50; pollTimeout=null +Feb-02 16:20:48.767 [main] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'FileTransfer' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:20:48.804 [main] DEBUG nextflow.cli.CmdRun - + Version: 23.10.1 build 5891 + Created: 12-01-2024 22:01 UTC (22:01 GMT) + System: Linux 5.15.133.1-microsoft-standard-WSL2 + Runtime: Groovy 3.0.19 on OpenJDK 64-Bit Server VM 17.0.10-internal+0-adhoc..src + Encoding: UTF-8 (UTF-8) + Process: 19107@b23599a75663 [172.17.0.3] + CPUs: 8 - Mem: 7.6 GB (781.6 MB) - Swap: 2 GB (2 GB) +Feb-02 16:20:48.855 [main] DEBUG nextflow.Session - Work-dir: /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work [ext2/ext3] +Feb-02 16:20:48.883 [main] DEBUG nextflow.executor.ExecutorFactory - Extension executors providers=[] +Feb-02 16:20:48.900 [main] DEBUG nextflow.Session - Observer factory: DefaultObserverFactory +Feb-02 16:20:49.006 [main] DEBUG nextflow.cache.CacheFactory - Using Nextflow cache factory: nextflow.cache.DefaultCacheFactory +Feb-02 16:20:49.019 [main] DEBUG nextflow.util.CustomThreadPool - Creating default thread pool > poolSize: 9; maxThreads: 1000 +Feb-02 16:20:49.166 [main] DEBUG nextflow.Session - Session start +Feb-02 16:20:49.176 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow started -- trace file: /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/trace.csv +Feb-02 16:20:49.187 [main] DEBUG nextflow.Session - Using default localLib path: /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib +Feb-02 16:20:49.193 [main] DEBUG nextflow.Session - Adding to the classpath library: /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/lib +Feb-02 16:20:49.654 [main] DEBUG nextflow.script.ScriptRunner - > Launching execution +Feb-02 16:20:49.982 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-amazon version: 2.1.4 +Feb-02 16:20:49.989 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-amazon@2.1.4' resolved +Feb-02 16:20:49.989 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-amazon@2.1.4' +Feb-02 16:20:50.015 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-amazon@2.1.4 +Feb-02 16:20:50.042 [main] DEBUG nextflow.file.FileHelper - > Added 'S3FileSystemProvider' to list of installed providers [s3] +Feb-02 16:20:50.043 [main] DEBUG nextflow.file.FileHelper - Started plugin 'nf-amazon' required to handle file: s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_1.fastq.gz +Feb-02 16:20:50.054 [main] DEBUG nextflow.file.FileHelper - Creating a file system instance for provider: S3FileSystemProvider +Feb-02 16:20:50.094 [main] DEBUG nextflow.cloud.aws.config.AwsConfig - AWS S3 config properties: {max_error_retry=5} +Feb-02 16:20:51.037 [main] DEBUG nextflow.cloud.aws.nio.S3Client - Setting S3 glacierRetrievalTier=null +Feb-02 16:20:51.130 [main] DEBUG n.c.aws.util.S3CredentialsProvider - No AWS credentials available - falling back to anonymous access +Feb-02 16:20:53.058 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name FASTQC +Feb-02 16:20:53.060 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:FASTQC` matches process FASTQC +Feb-02 16:20:53.061 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process FASTQC +Feb-02 16:20:53.071 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Feb-02 16:20:53.071 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Feb-02 16:20:53.081 [main] DEBUG nextflow.executor.Executor - [warm up] executor > local +Feb-02 16:20:53.088 [main] DEBUG n.processor.LocalPollingMonitor - Creating local task monitor for executor 'local' > cpus=8; memory=7.6 GB; capacity=8; pollInterval=100ms; dumpInterval=5m +Feb-02 16:20:53.090 [main] DEBUG n.processor.TaskPollingMonitor - >>> barrier register (monitor: local) +Feb-02 16:20:53.228 [main] DEBUG nextflow.Session - Config process names validation disabled as requested +Feb-02 16:20:53.230 [main] DEBUG nextflow.Session - Igniting dataflow network (2) +Feb-02 16:20:53.232 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > FASTQC +Feb-02 16:20:53.235 [main] DEBUG nextflow.script.ScriptRunner - Parsed script files: + Script_8a666d0da9535f00: /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/mock.nf + Script_4ba798786ecd6418: /workspaces/readsimulator/./modules/nf-core/fastqc/tests/../main.nf +Feb-02 16:20:53.235 [main] DEBUG nextflow.script.ScriptRunner - > Awaiting termination +Feb-02 16:20:53.235 [main] DEBUG nextflow.Session - Session await +Feb-02 16:20:53.543 [FileTransfer-1] DEBUG nextflow.file.FilePorter - Copying foreign file s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_1.fastq.gz to work dir: /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work/stage-d43840f8-afdc-4db7-8003-016db54c90d9/ed/26d185524da644c16b66a48f1ac480/test_1.fastq.gz +Feb-02 16:20:53.744 [FileTransfer-1] DEBUG n.cloud.aws.nio.S3FileSystemProvider - S3 download file from=s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_1.fastq.gz to=/workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work/stage-d43840f8-afdc-4db7-8003-016db54c90d9/ed/26d185524da644c16b66a48f1ac480/test_1.fastq.gz +Feb-02 16:20:53.744 [FileTransfer-1] DEBUG nextflow.cloud.aws.nio.S3Client - Creating S3 transfer manager pool - chunk-size=104857600; max-treads=10; +Feb-02 16:20:53.745 [FileTransfer-1] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'S3TransferManager' minSize=10; maxSize=10; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:20:54.391 [Actor Thread 7] WARN nextflow.container.SingularityCache - Singularity cache directory has not been defined -- Remote image will be stored in the path: /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work/singularity -- Use the environment variable NXF_SINGULARITY_CACHEDIR to specify a different location +Feb-02 16:20:54.391 [Actor Thread 7] INFO nextflow.container.SingularityCache - Pulling Singularity image https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0 [cache /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work/singularity/depot.galaxyproject.org-singularity-fastqc-0.12.1--hdfd78af_0.img] +Feb-02 16:20:55.612 [Actor Thread 7] DEBUG nextflow.container.SingularityCache - Singularity pull complete image=https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0 path=/workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work/singularity/depot.galaxyproject.org-singularity-fastqc-0.12.1--hdfd78af_0.img +Feb-02 16:20:55.746 [Task submitter] DEBUG n.executor.local.LocalTaskHandler - Launch cmd line: /bin/bash -ue .command.run +Feb-02 16:20:55.749 [Task submitter] INFO nextflow.Session - [69/e8ebc7] Submitted process > FASTQC (mysample) +Feb-02 16:21:00.142 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[id: 1; name: FASTQC (mysample); status: COMPLETED; exit: 0; error: -; workDir: /workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work/69/e8ebc7e72debe0dd0580d77be71355] +Feb-02 16:21:00.182 [Task monitor] WARN nextflow.processor.PublishDir - Process `FASTQC` publishDir path contains a variable with a null value +Feb-02 16:21:00.186 [Task monitor] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'PublishDir' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:21:00.192 [main] DEBUG nextflow.Session - Session await > all processes finished +Feb-02 16:21:00.196 [Task monitor] DEBUG n.processor.TaskPollingMonitor - <<< barrier arrives (monitor: local) - terminating tasks monitor poll loop +Feb-02 16:21:00.196 [main] DEBUG nextflow.Session - Session await > all barriers passed +Feb-02 16:21:00.199 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'PublishDir' shutdown completed (hard=false) +Feb-02 16:21:00.212 [main] DEBUG n.trace.WorkflowStatsObserver - Workflow completed > WorkflowStats[succeededCount=1; failedCount=0; ignoredCount=0; cachedCount=0; pendingCount=0; submittedCount=0; runningCount=0; retriesCount=0; abortedCount=0; succeedDuration=8s; failedDuration=0ms; cachedDuration=0ms;loadCpus=0; loadMemory=0; peakRunning=1; peakCpus=2; peakMemory=3 GB; ] +Feb-02 16:21:00.213 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow completed -- saving trace file +Feb-02 16:21:00.284 [main] DEBUG nextflow.cache.CacheDB - Closing CacheDB done +Feb-02 16:21:00.285 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-amazon@2.1.4' +Feb-02 16:21:00.285 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-amazon +Feb-02 16:21:00.285 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-validation@1.1.3' +Feb-02 16:21:00.285 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-validation +Feb-02 16:21:00.306 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'FileTransfer' shutdown completed (hard=false) +Feb-02 16:21:00.306 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'S3TransferManager' shutdown completed (hard=false) +Feb-02 16:21:00.306 [main] DEBUG nextflow.script.ScriptRunner - > Execution complete -- Goodbye diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_0.json b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_0.json new file mode 100644 index 0000000..ea170f5 --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_0.json @@ -0,0 +1 @@ +{"0":[[{"id":"mysample","single_end":true},"/workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work/69/e8ebc7e72debe0dd0580d77be71355/mysample_fastqc.html"]]} \ No newline at end of file diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_1.json b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_1.json new file mode 100644 index 0000000..a9a6ce7 --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_1.json @@ -0,0 +1 @@ +{"1":[[{"id":"mysample","single_end":true},"/workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work/69/e8ebc7e72debe0dd0580d77be71355/mysample_fastqc.zip"]]} \ No newline at end of file diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_2.json b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_2.json new file mode 100644 index 0000000..b175f95 --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_2.json @@ -0,0 +1 @@ +{"2":["/workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work/69/e8ebc7e72debe0dd0580d77be71355/versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_html.json b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_html.json new file mode 100644 index 0000000..9cb464a --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_html.json @@ -0,0 +1 @@ +{"html":[[{"id":"mysample","single_end":true},"/workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work/69/e8ebc7e72debe0dd0580d77be71355/mysample_fastqc.html"]]} \ No newline at end of file diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_versions.json b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_versions.json new file mode 100644 index 0000000..03eb209 --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_versions.json @@ -0,0 +1 @@ +{"versions":["/workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work/69/e8ebc7e72debe0dd0580d77be71355/versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_zip.json b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_zip.json new file mode 100644 index 0000000..8e8d33a --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/output_zip.json @@ -0,0 +1 @@ +{"zip":[[{"id":"mysample","single_end":true},"/workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/work/69/e8ebc7e72debe0dd0580d77be71355/mysample_fastqc.zip"]]} \ No newline at end of file diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/params.json b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/params.json new file mode 100644 index 0000000..b678130 --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/params.json @@ -0,0 +1 @@ +{"nf_test_output":"/workspaces/readsimulator/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta"} \ No newline at end of file diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/std.err b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/std.err new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/std.out b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/std.out new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/trace.csv b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/trace.csv new file mode 100644 index 0000000..7d3658c --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/trace.csv @@ -0,0 +1,2 @@ +task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar +1 69/e8ebc7 19294 FASTQC (mysample) COMPLETED 0 2024-02-02 16:20:55.748 4.4s 4s 101.3% 226 MB 3.8 GB 11.1 MB 2 MB diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/workflow.json b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/workflow.json new file mode 100644 index 0000000..7d54df0 --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/meta/workflow.json @@ -0,0 +1 @@ +{"success":true,"exitStatus":0} \ No newline at end of file diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/null/fastqc/mysample_fastqc.html b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/null/fastqc/mysample_fastqc.html new file mode 100644 index 0000000..4820907 --- /dev/null +++ b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/null/fastqc/mysample_fastqc.html @@ -0,0 +1,187 @@ +mysample.gz FastQC Report
    FastQCFastQC Report
    Fri 2 Feb 2024
    mysample.gz

    Summary

    [OK]Basic Statistics

    MeasureValue
    Filenamemysample.gz
    File typeConventional base calls
    EncodingSanger / Illumina 1.9
    Total Sequences100
    Total Bases13.8 kbp
    Sequences flagged as poor quality0
    Sequence length72-151
    %GC39

    [OK]Per base sequence quality

    Per base quality graph

    [FAIL]Per tile sequence quality

    Per tile quality graph

    [OK]Per sequence quality scores

    Per Sequence quality graph

    [FAIL]Per base sequence content

    Per base sequence content

    [FAIL]Per sequence GC content

    Per sequence GC content graph

    [OK]Per base N content

    N content graph

    [WARN]Sequence Length Distribution

    Sequence length distribution

    [OK]Sequence Duplication Levels

    Duplication level graph

    [FAIL]Overrepresented sequences

    SequenceCountPercentagePossible Source
    CAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGA22.0No Hit
    ACTCTAGATAATTCATTAGGTTTCTTAATAGTAAGACTAGAATTGTCTAC11.0No Hit
    GTCTAATCATAATTTCTTGGTACAGGCTGGTATTGTTCATCTCAGGGTTA11.0No Hit
    TGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTA11.0No Hit
    TTTCAAGACTTGTAAATTGCAACTTGTCATAAAGGTCTCTATCAGACATT11.0No Hit
    TTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTA11.0No Hit
    ATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTA11.0No Hit
    GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT11.0No Hit
    AAGTGTATCTTGATCCTCATAACTCATTGAATCATAATAAAGTCTAGCCT11.0No Hit
    GTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTC11.0No Hit
    AATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTA11.0No Hit
    CAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAAT11.0No Hit
    GTTCCTAGGTAGTAGAAATACCATCTAGGACTGAGATCTTTCATTTTACA11.0No Hit
    CATCTTATTTCTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGA11.0No Hit
    TATCTGCCCATTTAATAGAAGTTAAACCATTAACTTGTGGGTATTTCCAC11.0No Hit
    CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT11.0No Hit
    ACTGAATTTTCTGCACCAAGTGACATAGTGTAGGCAATGATGGATTGACT11.0No Hit
    AACTAGCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGC11.0No Hit
    AAGTTGGCGTATACGCGTAATATATCTGGGTTTTCTACAAAATCATACCA11.0No Hit
    TTCCAGAAGAGCAAGGTTCTTTTAAAAGTACTGTTGTACCTCTAACACAC11.0No Hit
    TATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAA11.0No Hit
    TTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTAATGGGTAA11.0No Hit
    GGTGTATTCAAGGCTCCCTCAGTTGCAACCCATATGATGCCGTCTTTGTT11.0No Hit
    GTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTA11.0No Hit
    AAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTT11.0No Hit
    ACATAGTGTAGGCAATGATGGATTGACTAGCTACACTACGTGCCCGCCGA11.0No Hit
    GTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAA11.0No Hit
    CAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCT11.0No Hit
    GGATAGACAGATCGAATTCTACCCATAAAGCCATCAAGCTTTTTCTTTTC11.0No Hit
    ACTGTTTTCTTTGTAGAAAACATCCGTAATAGGACCTTTGTATTCTGAGG11.0No Hit
    GATGAAACATCATGCGTGATAACACCCTTATAAAACATTTTAAAGCATTG11.0No Hit
    GTTATAACACTGACACCACCAAAAGAACATGGTGTAATGTCAAGAATCTC11.0No Hit
    GCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGT11.0No Hit
    CCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGAC11.0No Hit
    CAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCT11.0No Hit
    CATTAGCACAAGTTGTAGGTATTTGTACATACTTACCTTTTAAGTCACAA11.0No Hit
    GTTACTCACAATTTTGACTTCACTTTTAGTCTTAGTCCAGAGTACTCAAT11.0No Hit
    TGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTG11.0No Hit
    CTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTG11.0No Hit
    TCTTTGTAAGCTTTAGCAGCATCTACAGCAAAAGCACAGAAAGATAATAC11.0No Hit
    ATGTCAGATTTATGTGACAATTTAAGACATTCAACAACATCTTTAGTTTC11.0No Hit
    GGATTAGTAACACTACAGCTGATGCATACATAACACAGTCTTTTAGCTTA11.0No Hit
    CTGTAATAGTTGTGTCCGTACCAGCTGCTTGTGCTGTTTGCCTGTCAACA11.0No Hit
    GAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTG11.0No Hit
    CTTTTACAAGACTTCAGAGTTTAGAAATTGTGGCTTATAATGTTGTAATT11.0No Hit
    TGGCAATAGAGTTATTAGAGTAAGCAACTGAATTTTCTGCACCAAGTGAC11.0No Hit
    CACAAACTCTAAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCAGAT11.0No Hit
    CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGA11.0No Hit
    AACACCAGCTTCTGATCTTTCACAAGTGCCGTGCCTACAGTACTCAGAAT11.0No Hit
    GATTTTTAAGTGTGTCACTTAACATTTGTACAATCTTTATACGCACTACA11.0No Hit
    AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC11.0No Hit
    AGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTA11.0No Hit
    CATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTT11.0No Hit
    GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG11.0No Hit
    ACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAA11.0No Hit
    CACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATA11.0No Hit
    TGCCAATCCTGTAGCGACTGTATGCAGCAAAACCTGAGTCACCTGCTACA11.0No Hit
    GATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGC11.0No Hit
    GTACGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTA11.0No Hit
    CTATAACATTAATAGGCAATGAACCTTTAGTGTTATTAGCTCTCAGGTTG11.0No Hit
    ATAAACATGTTAGGGTAACCATTAACTTGATAATTCATTTTAAAACCCAT11.0No Hit
    CCAAGCAGGGTTACGTGTAAGGAATTCTCTTACCACGCCTATTTGTGGCC11.0No Hit
    GATTTAGCCACATTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGA11.0No Hit
    GTTGAAAGTAGATCTTCAATAAATGACCTCTTGCTTGGTTTTGATGGATC11.0No Hit
    TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCG11.0No Hit
    TAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCG11.0No Hit
    GCTATAACGCAGCCTGTAAAATCATCTGGTAATTTATAATTATAATCAGC11.0No Hit
    AGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTT11.0No Hit
    TCTAAGCATAGTGAAAAGCATTGTCTGCATAGCACTAGTAACTTTTGCCC11.0No Hit
    GAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGA11.0No Hit
    ATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGAT11.0No Hit
    CCGTCTATGCAATACAAAGTTTCTTTAGAAGTTATATGTTTATAGTGACC11.0No Hit
    GCAATTCATAGCTCTTTTCAGAACGTTCCGTGTACCAAGCAATTTCATGC11.0No Hit
    AGTAAACTGTTACAAACAACAACAGCAAGTTGCAAACAAAGTGAACACCC11.0No Hit
    GTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAG11.0No Hit
    GTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTG11.0No Hit
    CTCCAAAGCCACGTACGAGCACGTCGCGAACCTGTAAAACAGGCAAACTG11.0No Hit
    GTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCA11.0No Hit
    GTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAATTGTCTGT11.0No Hit
    GTACAAAAATAGCCTAAGAAACAATAAACTAGCATTATACACTGAAGTGT11.0No Hit
    GATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACA11.0No Hit
    GTGAAATTAGTATGGACAAATCACCTAATTTAGCATGGCCTCTTATTGTA11.0No Hit
    TCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAG11.0No Hit
    GCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGC11.0No Hit
    TGCGTCTTGGGTCCCCGCTCTCACTCAACATGGCAAGGAAGACCTTAAAT11.0No Hit
    AACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCA11.0No Hit
    CTAAAAGTGAAGTCAAAATTGTGAGTAACAACCAGTGGTGTGTACCCGTG11.0No Hit
    ATGTAAGGCTTTGTTAAGTCAGTGTCAACATGTGACTCTGCAGTTAAAGC11.0No Hit
    CCTTGAATGAGTCTAATTCAGGTTGCAAAGGATCATAAACTGTGTTGTTG11.0No Hit
    GCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGC11.0No Hit
    ATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGG11.0No Hit
    GCTGCTAGAGACCTCGTTTGTGCACAAAAGTTTAACGGCCTTACTGTTTT11.0No Hit
    CCCTTTGTTGTGTTGTAGTAAGCTAACGCATTGTCATCAGTGCAAGCAGT11.0No Hit
    ATCCTTTAGGATTTGGATGATCTATGTGGCAACGGCAGTACAGACAACAC11.0No Hit
    CTACACGAGCACGTGCAGGTATAATTCTACTACATTTATCTATAGGCAAA11.0No Hit
    TGCCTGACCAGTACCAGTGTGTGTACACAACATCTTAACACAATTAGTGA11.0No Hit
    GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT11.0No Hit
    ATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTT11.0No Hit
    TTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATA11.0No Hit

    [OK]Adapter Content

    Adapter graph

    Produced by FastQC (version 0.12.1)
    \ No newline at end of file diff --git a/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/null/fastqc/mysample_fastqc.zip b/.nf-test/tests/3ed79c9acc8d86b48d7d3fdbb55745c5/null/fastqc/mysample_fastqc.zip new file mode 100644 index 0000000000000000000000000000000000000000..25fbaa8072413eaca6da956fbf17e68833b3dd0c GIT binary patch literal 454737 zcmce;1#l!wvLz~Jh88n3vs%n*F*7qWGc!vqR*RXLnVFfHnc?f+nYT7?ZD#*p`)gZS zQCS&r+|9!y>Ud_PSISBPgP;IFKtKRw_zS24{L2gB`@5B!gPxVGrIC)Yo`a*E0Ua=a ztRy(ZUm&1=B4z)h{EvSlFK%FC?eM>)1?}HyvC=a!`rlJS^6%9AZOlJT3H2=mD=sD^|Gmfmt3ZH#e>%9k-+%8w90bG_zx@W`31JxeeGF+U zrsnXCU;3*63XrF}epg~R3adIQ*cdyy=-C?qxVX5`nps;o80gs=(c0LXq+fDlek-CB z7vfh`4XQ}iGR0Ox8S*hPx>8IJ%$rB5W*10f-tt0?up+DI`|F_WJjv)4DCpRm%lBEIW4 zw)wGjca!Zg_46sTv=-$AsbrErqF1Urs2jAK*%k~Pj0YTydf_JMeOMoInb4_Qdz(O4 z3Gsn?S^;e8O8LWM1;jEA%eSw4by_4EMtEn^t8@zsGE@vz7rXQ|9_t-b7l2&L&{jqo zIGtu;>s^;P(x^!c=sAb)THEh}L>4ay%db55@_^ERHn@4w()55y4hJ zqTlz)SHm9+sHvgsF@#h%LdZAr4b=N((Z5jFHhTjzV@>vl>g%w4CXwA 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%>Pipeline completed successfully!<% } else { %>Pipeline completed with errors. The full error message was: ${errorReport}.<% } %>", + "wrap": true + }, + { + "type": "TextBlock", + "text": "The command used to launch the workflow was as follows:", + "wrap": true + }, + { + "type": "TextBlock", + "text": "${commandLine}", + "isSubtle": true, + "wrap": true + } + ], + "actions": [ + { + "type": "Action.ShowCard", + "title": "Pipeline Configuration", + "card": { + "type": "AdaptiveCard", + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "body": [ + { + "type": "FactSet", + "facts": [<% out << summary.collect{ k,v -> "{\"title\": \"$k\", \"value\" : \"$v\"}"}.join(",\n") %> + ] + } + ] + } + } + ] + } + } + ] +} diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/email_template.html b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/email_template.html new file mode 100644 index 0000000..f2bc049 --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/email_template.html @@ -0,0 +1,53 @@ + + + + + + + + nf-core/readsimulator Pipeline Report + + +

    + + + diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/email_template.txt b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/email_template.txt new file mode 100644 index 0000000..6ca5507 --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/email_template.txt @@ -0,0 +1,39 @@ +---------------------------------------------------- + ,--./,-. + ___ __ __ __ ___ /,-._.--~\\ + |\\ | |__ __ / ` / \\ |__) |__ } { + | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, + `._,._,' + nf-core/readsimulator ${version} +---------------------------------------------------- +Run Name: $runName + +<% if (success){ + out << "## nf-core/readsimulator execution completed successfully! ##" +} else { + out << """#################################################### +## nf-core/readsimulator execution completed unsuccessfully! ## +#################################################### +The exit status of the task that caused the workflow execution to fail was: $exitStatus. +The full error message was: + +${errorReport} +""" +} %> + + +The workflow was completed at $dateComplete (duration: $duration) + +The command used to launch the workflow was as follows: + + $commandLine + + + +Pipeline Configuration: +----------------------- +<% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> + +-- +nf-core/readsimulator +https://github.com/nf-core/readsimulator diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/methods_description_template.yml b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/methods_description_template.yml new file mode 100644 index 0000000..6119677 --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/methods_description_template.yml @@ -0,0 +1,29 @@ +id: "nf-core-readsimulator-methods-description" +description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." +section_name: "nf-core/readsimulator Methods Description" +section_href: "https://github.com/nf-core/readsimulator" +plot_type: "html" +## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline +## You inject any metadata in the Nextflow '${workflow}' object +data: | +

    Methods

    +

    Data was processed using nf-core/readsimulator v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

    +

    The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

    + 
    ${workflow.commandLine}
    +

    ${tool_citations}

    +

    References

    +
      +
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
      • +
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
      • +
    • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
      • +
    • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
      • + ${tool_bibliography} +
    +
    +
    Notes:
    +
      + ${nodoi_text} +
    • The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
      • +
    • You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.
      • +
    +
    diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/multiqc_config.yml b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/multiqc_config.yml new file mode 100644 index 0000000..7d3650d --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/multiqc_config.yml @@ -0,0 +1,15 @@ +report_comment: > + + This report has been generated by the nf-core/readsimulator + analysis pipeline. 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charset=utf-8 + +$email_html + +--nfcoremimeboundary +Content-Type: image/png;name="nf-core-readsimulator_logo.png" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: inline; filename="nf-core-readsimulator_logo_light.png" + +<% out << new File("$projectDir/assets/nf-core-readsimulator_logo_light.png"). + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' ) %> + +<% +if (mqcFile){ +def mqcFileObj = new File("$mqcFile") +if (mqcFileObj.length() < mqcMaxSize){ +out << """ +--nfcoremimeboundary +Content-Type: text/html; name=\"multiqc_report\" +Content-Transfer-Encoding: base64 +Content-ID: +Content-Disposition: attachment; filename=\"${mqcFileObj.getName()}\" + +${mqcFileObj. + bytes. + encodeBase64(). + toString(). + tokenize( '\n' )*. + toList()*. + collate( 76 )*. + collect { it.join() }. + flatten(). + join( '\n' )} +""" +}} +%> + +--nfcoremimeboundary-- diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/slackreport.json b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/slackreport.json new file mode 100644 index 0000000..e9d5f8a --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/assets/slackreport.json @@ -0,0 +1,34 @@ +{ + "attachments": [ + { + "fallback": "Plain-text summary of the attachment.", + "color": "<% if (success) { %>good<% } else { %>danger<%} %>", + "author_name": "nf-core/readsimulator ${version} - ${runName}", + "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", + "fields": [ + { + "title": "Command used to launch the workflow", + "value": "```${commandLine}```", + "short": false + } + <% + if (!success) { %> + , + { + "title": "Full error message", + "value": "```${errorReport}```", + "short": false + }, + { + "title": "Pipeline configuration", + "value": "<% out << summary.collect{ k,v -> k == "hook_url" ? "_${k}_: (_hidden_)" : ( ( v.class.toString().contains('Path') || ( v.class.toString().contains('String') && v.contains('/') ) ) ? "_${k}_: `${v}`" : (v.class.toString().contains('DateTime') ? ("_${k}_: " + v.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM))) : "_${k}_: ${v}") ) }.join(",\n") %>", + "short": false + } + <% } + %> + ], + "footer": "Completed at <% out << dateComplete.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM)) %> (duration: ${duration})" + } + ] +} diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/bin/check_samplesheet.py b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/bin/check_samplesheet.py new file mode 100755 index 0000000..4a758fe --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/bin/check_samplesheet.py @@ -0,0 +1,259 @@ +#!/usr/bin/env python + + +"""Provide a command line tool to validate and transform tabular samplesheets.""" + + +import argparse +import csv +import logging +import sys +from collections import Counter +from pathlib import Path + +logger = logging.getLogger() + + +class RowChecker: + """ + Define a service that can validate and transform each given row. + + Attributes: + modified (list): A list of dicts, where each dict corresponds to a previously + validated and transformed row. The order of rows is maintained. + + """ + + VALID_FORMATS = ( + ".fq.gz", + ".fastq.gz", + ) + + def __init__( + self, + sample_col="sample", + first_col="fastq_1", + second_col="fastq_2", + single_col="single_end", + **kwargs, + ): + """ + Initialize the row checker with the expected column names. + + Args: + sample_col (str): The name of the column that contains the sample name + (default "sample"). + first_col (str): The name of the column that contains the first (or only) + FASTQ file path (default "fastq_1"). + second_col (str): The name of the column that contains the second (if any) + FASTQ file path (default "fastq_2"). + single_col (str): The name of the new column that will be inserted and + records whether the sample contains single- or paired-end sequencing + reads (default "single_end"). + + """ + super().__init__(**kwargs) + self._sample_col = sample_col + self._first_col = first_col + self._second_col = second_col + self._single_col = single_col + self._seen = set() + self.modified = [] + + def validate_and_transform(self, row): + """ + Perform all validations on the given row and insert the read pairing status. + + Args: + row (dict): A mapping from column headers (keys) to elements of that row + (values). + + """ + self._validate_sample(row) + self._validate_first(row) + self._validate_second(row) + self._validate_pair(row) + self._seen.add((row[self._sample_col], row[self._first_col])) + self.modified.append(row) + + def _validate_sample(self, row): + """Assert that the sample name exists and convert spaces to underscores.""" + if len(row[self._sample_col]) <= 0: + raise AssertionError("Sample input is required.") + # Sanitize samples slightly. + row[self._sample_col] = row[self._sample_col].replace(" ", "_") + + def _validate_first(self, row): + """Assert that the first FASTQ entry is non-empty and has the right format.""" + if len(row[self._first_col]) <= 0: + raise AssertionError("At least the first FASTQ file is required.") + self._validate_fastq_format(row[self._first_col]) + + def _validate_second(self, row): + """Assert that the second FASTQ entry has the right format if it exists.""" + if len(row[self._second_col]) > 0: + self._validate_fastq_format(row[self._second_col]) + + def _validate_pair(self, row): + """Assert that read pairs have the same file extension. Report pair status.""" + if row[self._first_col] and row[self._second_col]: + row[self._single_col] = False + first_col_suffix = Path(row[self._first_col]).suffixes[-2:] + second_col_suffix = Path(row[self._second_col]).suffixes[-2:] + if first_col_suffix != second_col_suffix: + raise AssertionError("FASTQ pairs must have the same file extensions.") + else: + row[self._single_col] = True + + def _validate_fastq_format(self, filename): + """Assert that a given filename has one of the expected FASTQ extensions.""" + if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): + raise AssertionError( + f"The FASTQ file has an unrecognized extension: {filename}\n" + f"It should be one of: {', '.join(self.VALID_FORMATS)}" + ) + + def validate_unique_samples(self): + """ + Assert that the combination of sample name and FASTQ filename is unique. + + In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the + number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. + + """ + if len(self._seen) != len(self.modified): + raise AssertionError("The pair of sample name and FASTQ must be unique.") + seen = Counter() + for row in self.modified: + sample = row[self._sample_col] + seen[sample] += 1 + row[self._sample_col] = f"{sample}_T{seen[sample]}" + + +def read_head(handle, num_lines=10): + """Read the specified number of lines from the current position in the file.""" + lines = [] + for idx, line in enumerate(handle): + if idx == num_lines: + break + lines.append(line) + return "".join(lines) + + +def sniff_format(handle): + """ + Detect the tabular format. + + Args: + handle (text file): A handle to a `text file`_ object. The read position is + expected to be at the beginning (index 0). + + Returns: + csv.Dialect: The detected tabular format. + + .. _text file: + https://docs.python.org/3/glossary.html#term-text-file + + """ + peek = read_head(handle) + handle.seek(0) + sniffer = csv.Sniffer() + dialect = sniffer.sniff(peek) + return dialect + + +def check_samplesheet(file_in, file_out): + """ + Check that the tabular samplesheet has the structure expected by nf-core pipelines. + + Validate the general shape of the table, expected columns, and each row. Also add + an additional column which records whether one or two FASTQ reads were found. + + Args: + file_in (pathlib.Path): The given tabular samplesheet. The format can be either + CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. + file_out (pathlib.Path): Where the validated and transformed samplesheet should + be created; always in CSV format. + + Example: + This function checks that the samplesheet follows the following structure, + see also the `viral recon samplesheet`_:: + + sample,fastq_1,fastq_2 + SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz + SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz + SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + + .. _viral recon samplesheet: + https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + + """ + required_columns = {"sample", "fastq_1", "fastq_2"} + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_in.open(newline="") as in_handle: + reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) + # Validate the existence of the expected header columns. + if not required_columns.issubset(reader.fieldnames): + req_cols = ", ".join(required_columns) + logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") + sys.exit(1) + # Validate each row. + checker = RowChecker() + for i, row in enumerate(reader): + try: + checker.validate_and_transform(row) + except AssertionError as error: + logger.critical(f"{str(error)} On line {i + 2}.") + sys.exit(1) + checker.validate_unique_samples() + header = list(reader.fieldnames) + header.insert(1, "single_end") + # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. + with file_out.open(mode="w", newline="") as out_handle: + writer = csv.DictWriter(out_handle, header, delimiter=",") + writer.writeheader() + for row in checker.modified: + writer.writerow(row) + + +def parse_args(argv=None): + """Define and immediately parse command line arguments.""" + parser = argparse.ArgumentParser( + description="Validate and transform a tabular samplesheet.", + epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", + ) + parser.add_argument( + "file_in", + metavar="FILE_IN", + type=Path, + help="Tabular input samplesheet in CSV or TSV format.", + ) + parser.add_argument( + "file_out", + metavar="FILE_OUT", + type=Path, + help="Transformed output samplesheet in CSV format.", + ) + parser.add_argument( + "-l", + "--log-level", + help="The desired log level (default WARNING).", + choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), + default="WARNING", + ) + return parser.parse_args(argv) + + +def main(argv=None): + """Coordinate argument parsing and program execution.""" + args = parse_args(argv) + logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") + if not args.file_in.is_file(): + logger.error(f"The given input file {args.file_in} was not found!") + sys.exit(2) + args.file_out.parent.mkdir(parents=True, exist_ok=True) + check_samplesheet(args.file_in, args.file_out) + + +if __name__ == "__main__": + sys.exit(main()) diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/NfcoreTemplate.groovy b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/NfcoreTemplate.groovy new file mode 100755 index 0000000..e248e4c --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/NfcoreTemplate.groovy @@ -0,0 +1,356 @@ +// +// This file holds several functions used within the nf-core pipeline template. +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +class NfcoreTemplate { + + // + // Check AWS Batch related parameters have been specified correctly + // + public static void awsBatch(workflow, params) { + if (workflow.profile.contains('awsbatch')) { + // Check params.awsqueue and params.awsregion have been set if running on AWSBatch + assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" + // Check outdir paths to be S3 buckets if running on AWSBatch + assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" + } + } + + // + // Warn if a -profile or Nextflow config has not been provided to run the pipeline + // + public static void checkConfigProvided(workflow, log) { + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + } + } + + // + // Generate version string + // + public static String version(workflow) { + String version_string = "" + + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string + } + + // + // Construct and send completion email + // + public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = NfcoreTemplate.version(workflow) + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + + // Check if we are only sending emails on failure + def email_address = params.email + if (!params.email && params.email_on_fail && !workflow.success) { + email_address = params.email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("$projectDir/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("$projectDir/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("$projectDir/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(params.monochrome_logs) + if (email_address) { + try { + if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + mail_cmd += [ '-A', mqc_report ] + } + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() + } + + // + // Construct and send a notification to a web server as JSON + // e.g. Microsoft Teams and Slack + // + public static void IM_notification(workflow, params, summary_params, projectDir, log) { + def hook_url = params.hook_url + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = NfcoreTemplate.version(workflow) + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("$projectDir/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } + } + + // + // Dump pipeline parameters in a json file + // + public static void dump_parameters(workflow, params) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() + } + + // + // Print pipeline summary on completion + // + public static void summary(workflow, params, log) { + Map colors = logColours(params.monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + } + + // + // ANSII Colours used for terminal logging + // + public static Map logColours(Boolean monochrome_logs) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes + } + + // + // Does what is says on the tin + // + public static String dashedLine(monochrome_logs) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" + } + + // + // nf-core logo + // + public static String logo(workflow, monochrome_logs) { + Map colors = logColours(monochrome_logs) + String workflow_version = NfcoreTemplate.version(workflow) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) + } +} diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/NftestUtils.groovy b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/NftestUtils.groovy new file mode 100644 index 0000000..ce6fc8b --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/NftestUtils.groovy @@ -0,0 +1,38 @@ +// Helper functions for pipeline tests + +class NftestUtils { + + // Function to remove Nextflow version from software_versions.yml + public static String removeNextflowVersion(outputDir) { + def softwareVersions = path("$outputDir/pipeline_info/software_versions.yml").yaml + if (softwareVersions.containsKey("Workflow")) { + softwareVersions.Workflow.remove("Nextflow") + } + return softwareVersions + } + + // Function to filter lines from a file and return a new file + public static File filterLines(String inFilePath, int linesToSkip) { + if (linesToSkip >= 0) { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lineCount = 0 + inputFile.eachLine { line -> + lineCount++ + if (lineCount > linesToSkip) { + outputFile.append(line + '\n') + } + } + return outputFile + } else { + File inputFile = new File(inFilePath) + File outputFile = new File(inFilePath + ".filtered") + def lines = inputFile.readLines() + def totalLines = lines.size() + lines.take(totalLines + linesToSkip).each { line -> + outputFile.append(line + '\n') + } + return outputFile + } + } +} diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/Utils.groovy b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/Utils.groovy new file mode 100644 index 0000000..8d030f4 --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/Utils.groovy @@ -0,0 +1,47 @@ +// +// This file holds several Groovy functions that could be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml + +class Utils { + + // + // When running with -profile conda, warn if channels have not been set-up appropriately + // + public static void checkCondaChannels(log) { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } + } +} diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/WorkflowMain.groovy b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/WorkflowMain.groovy new file mode 100755 index 0000000..477ddaf --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/WorkflowMain.groovy @@ -0,0 +1,77 @@ +// +// This file holds several functions specific to the main.nf workflow in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow + +class WorkflowMain { + + // + // Citation string for pipeline + // + public static String citation(workflow) { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + // TODO nf-core: Add Zenodo DOI for pipeline after first release + //"* The pipeline\n" + + //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + } + + + // + // Validate parameters and print summary to screen + // + public static void initialise(workflow, params, log, args) { + + // Print workflow version and exit on --version + if (params.version) { + String workflow_version = NfcoreTemplate.version(workflow) + log.info "${workflow.manifest.name} ${workflow_version}" + System.exit(0) + } + + // Check that a -profile or Nextflow config has been provided to run the pipeline + NfcoreTemplate.checkConfigProvided(workflow, log) + // Check that the profile doesn't contain spaces and doesn't end with a trailing comma + checkProfile(workflow.profile, args, log) + + // Check that conda channels are set-up correctly + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + Utils.checkCondaChannels(log) + } + + // Check AWS batch settings + NfcoreTemplate.awsBatch(workflow, params) + + // Check input has been provided + if (!params.input) { + Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") + } + } + // + // Get attribute from genome config file e.g. fasta + // + public static Object getGenomeAttribute(params, attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null + } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } +} diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/WorkflowReadsimulator.groovy b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/WorkflowReadsimulator.groovy new file mode 100755 index 0000000..c0b9df0 --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib/WorkflowReadsimulator.groovy @@ -0,0 +1,131 @@ +// +// This file holds several functions specific to the workflow/readsimulator.nf in the nf-core/readsimulator pipeline +// + +import nextflow.Nextflow +import groovy.text.SimpleTemplateEngine + +class WorkflowReadsimulator { + + // + // Check and validate parameters + // + public static void initialise(params, log) { + + genomeExistsError(params, log) + + if (!params.fasta && !params.ncbidownload_accessions && !params.ncbidownload_taxids) { + Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file. Alternatively, '--ncbidownload_accessions accessions.txt' or '--ncbidownload_taxids taxids.txt' can be used." + } + } + + // + // Get workflow summary for MultiQC + // + public static String paramsSummaryMultiqc(workflow, summary) { + String summary_section = '' + for (group in summary.keySet()) { + def group_params = summary.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + return yaml_file_text + } + + // + // Generate methods description for MultiQC + // + + public static String toolCitationText(params) { + def citation_text = [ + "Tools used in the workflow included:", + params["amplicon"] ? [ + "CRABS (Jeunen et al. 2022),", + "ART (Huang et al. 2012)," + ].join(' ').trim() : "", + params["target_capture"] ? [ + "Bowtie2 (Langmead et al. 2012),", + "Samtools (Danecek et al. 2021),", + "CapSim (Cao et al. 2018)," + ].join(' ').trim() : "", + params["metagenome"] ? + "InSilicoSeq (Gourlé et al. 2018)," : "", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text + } + + public static String toolBibliographyText(params) { + def reference_text = [ + params["amplicon"] ? [ + "
  • Jeunen, G.-J., Dowle, E., Edgecombe, J., von Ammon, U., Gemmell, N. J., & Cross, H. (2022). crabs—A software program to generate curated reference databases for metabarcoding sequencing data. Molecular Ecology Resources, 00, 1– 14. https://doi.org/10.1111/1755-0998.13741
    • ", + "
  • Weichun Huang, Leping Li, Jason R. Myers, Gabor T. Marth, ART: a next-generation sequencing read simulator, Bioinformatics, Volume 28, Issue 4, February 2012, Pages 593–594, https://doi.org/10.1093/bioinformatics/btr708
    • " + ].join(' ').trim() : "", + params["target_capture"] ? [ + "
  • Langmead, B., Salzberg, S. Fast gapped-read alignment with Bowtie 2. Nat Methods 9, 357–359 (2012). https://doi.org/10.1038/nmeth.1923
    • ", + "
  • Twelve years of SAMtools and BCFtools. Petr Danecek, James K Bonfield, Jennifer Liddle, John Marshall, Valeriu Ohan, Martin O Pollard, Andrew Whitwham, Thomas Keane, Shane A McCarthy, Robert M Davies, Heng Li. GigaScience, Volume 10, Issue 2, February 2021, giab008, https://doi.org/10.1093/gigascience/giab008
    • ", + "
  • Minh Duc Cao, Devika Ganesamoorthy, Chenxi Zhou, Lachlan J M Coin, Simulating the dynamics of targeted capture sequencing with CapSim, Bioinformatics, Volume 34, Issue 5, March 2018, Pages 873–874, https://doi.org/10.1093/bioinformatics/btx691
    • " + ].join(' ').trim() : "", + params["metagenome"] ? + "
  • Gourlé H, Karlsson-Lindsjö O, Hayer J and Bongcam+Rudloff E, Simulating Illumina data with InSilicoSeq. Bioinformatics (2018) doi:10.1093/bioinformatics/bty630
    • " : "", + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text + } + + public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = run_workflow.toMap() + meta["manifest_map"] = run_workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + meta["tool_bibliography"] = toolBibliographyText(params) + + + def methods_text = mqc_methods_yaml.text + + def engine = new SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html + } + + // + // Exit pipeline if incorrect --genome key provided + // + private static void genomeExistsError(params, log) { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + Nextflow.error(error_string) + } + } +} diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/mock.nf b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/mock.nf new file mode 100644 index 0000000..ea74244 --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/mock.nf @@ -0,0 +1,86 @@ +import groovy.json.JsonGenerator +import groovy.json.JsonGenerator.Converter + +nextflow.enable.dsl=2 + +// comes from nf-test to store json files +params.nf_test_output = "" + +// include dependencies + + +// include test process +include { FASTQC } from '/workspaces/readsimulator/./modules/nf-core/fastqc/tests/../main.nf' + +// define custom rules for JSON that will be generated. +def jsonOutput = + new JsonGenerator.Options() + .excludeNulls() // Do not include fields with value null.. + .addConverter(Path) { value -> value.toString() } // Custom converter for Path. Only filename + .build() + + +workflow { + + // run dependencies + + + // process mapping + def input = [] + + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + + //---- + + //run process + FASTQC(*input) + + if (FASTQC.output){ + + // consumes all named output channels and stores items in a json file + for (def name in FASTQC.out.getNames()) { + serializeChannel(name, FASTQC.out.getProperty(name), jsonOutput) + } + + // consumes all unnamed output channels and stores items in a json file + def array = FASTQC.out as Object[] + for (def i = 0; i < array.length ; i++) { + serializeChannel(i, array[i], jsonOutput) + } + + } + +} + +def serializeChannel(name, channel, jsonOutput) { + def _name = name + def list = [ ] + channel.subscribe( + onNext: { + list.add(it) + }, + onComplete: { + def map = new HashMap() + map[_name] = list + def filename = "${params.nf_test_output}/output_${_name}.json" + new File(filename).text = jsonOutput.toJson(map) + } + ) +} + + +workflow.onComplete { + + def result = [ + success: workflow.success, + exitStatus: workflow.exitStatus, + errorMessage: workflow.errorMessage, + errorReport: workflow.errorReport + ] + new File("${params.nf_test_output}/workflow.json").text = jsonOutput.toJson(result) + +} diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/nextflow.log b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/nextflow.log new file mode 100644 index 0000000..b301a58 --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/nextflow.log @@ -0,0 +1,102 @@ +Feb-02 16:19:28.810 [main] DEBUG nextflow.cli.Launcher - $> nextflow -quiet -log /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/nextflow.log run /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/mock.nf -c /workspaces/readsimulator/nextflow.config -c /workspaces/readsimulator/tests/nextflow.config -params-file /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/params.json -ansi-log false -profile singularity -with-trace /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/trace.csv -w /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work +Feb-02 16:19:28.922 [main] INFO nextflow.cli.CmdRun - N E X T F L O W ~ version 23.10.1 +Feb-02 16:19:28.952 [main] DEBUG nextflow.plugin.PluginsFacade - Setting up plugin manager > mode=prod; embedded=false; plugins-dir=/home/gitpod/.nextflow/plugins; core-plugins: nf-amazon@2.1.4,nf-azure@1.3.3,nf-cloudcache@0.3.0,nf-codecommit@0.1.5,nf-console@1.0.6,nf-ga4gh@1.1.0,nf-google@1.8.3,nf-tower@1.6.3,nf-wave@1.0.1 +Feb-02 16:19:28.967 [main] INFO o.pf4j.DefaultPluginStatusProvider - Enabled plugins: [] +Feb-02 16:19:28.969 [main] INFO o.pf4j.DefaultPluginStatusProvider - Disabled plugins: [] +Feb-02 16:19:28.974 [main] INFO org.pf4j.DefaultPluginManager - PF4J version 3.4.1 in 'deployment' mode +Feb-02 16:19:28.990 [main] INFO org.pf4j.AbstractPluginManager - No plugins +Feb-02 16:19:29.015 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /workspaces/readsimulator/nextflow.config +Feb-02 16:19:29.016 [main] DEBUG nextflow.config.ConfigBuilder - User config file: /workspaces/readsimulator/tests/nextflow.config +Feb-02 16:19:29.017 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /workspaces/readsimulator/nextflow.config +Feb-02 16:19:29.017 [main] DEBUG nextflow.config.ConfigBuilder - Parsing config file: /workspaces/readsimulator/tests/nextflow.config +Feb-02 16:19:29.054 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity` +Feb-02 16:19:33.075 [main] DEBUG nextflow.config.ConfigBuilder - Applying config profile: `singularity` +Feb-02 16:19:33.945 [main] DEBUG nextflow.config.ConfigBuilder - Available config profiles: [cfc_dev, uzl_omics, ifb_core, denbi_qbic, alice, mjolnir_globe, uppmax, incliva, ilifu, uge, rosalind_uge, lugh, unibe_ibu, vai, czbiohub_aws, jax, ccga_med, scw, tigem, tubingen_apg, google, apollo, ipop_up, pdc_kth, googlels, daisybio, eddie, medair, biowulf, apptainer, bi, bigpurple, adcra, cedars, pawsey_setonix, vsc_kul_uhasselt, pawsey_nimbus, ucl_myriad, utd_ganymede, charliecloud, icr_davros, ceres, munin, arm, rosalind, hasta, cfc, uzh, ebi_codon_slurm, ebc, ku_sund_dangpu, ccga_dx, crick, marvin, biohpc_gen, shifter, mana, mamba, unc_lccc, wehi, awsbatch, wustl_htcf, imperial, maestro, software_license, genotoul, nci_gadi, abims, janelia, nu_genomics, googlebatch, oist, sahmri, mpcdf, leicester, vsc_ugent, create, sage, cambridge, jex, podman, ebi_codon, cheaha, xanadu, nyu_hpc, test, computerome, ucd_sonic, seg_globe, sanger, dkfz, pasteur, ethz_euler, test_full, imb, tuos_stanage, azurebatch, hki, crukmi, csiro_petrichor, docker, engaging, gis, hypatia, psmn, eva, nygc, fgcz, conda, crg, singularity, self_hosted_runner, uw_hyak_pedslabs, utd_sysbio, debug, genouest, cbe, phoenix, gitpod, seawulf, uod_hpc, fub_curta, uct_hpc, aws_tower, binac] +Feb-02 16:19:34.010 [main] DEBUG nextflow.cli.CmdRun - Applied DSL=2 from script declararion +Feb-02 16:19:34.032 [main] INFO nextflow.cli.CmdRun - Launching `/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/mock.nf` [pedantic_gilbert] DSL2 - revision: 95dc6aa949 +Feb-02 16:19:34.032 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins declared=[nf-validation@1.1.3] +Feb-02 16:19:34.032 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins default=[] +Feb-02 16:19:34.032 [main] DEBUG nextflow.plugin.PluginsFacade - Plugins resolved requirement=[nf-validation@1.1.3] +Feb-02 16:19:34.033 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-validation version: 1.1.3 +Feb-02 16:19:34.040 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-validation@1.1.3' resolved +Feb-02 16:19:34.040 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-validation@1.1.3' +Feb-02 16:19:34.048 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-validation@1.1.3 +Feb-02 16:19:34.059 [main] DEBUG n.secret.LocalSecretsProvider - Secrets store: /home/gitpod/.nextflow/secrets/store.json +Feb-02 16:19:34.062 [main] DEBUG nextflow.secret.SecretsLoader - Discovered secrets providers: [nextflow.secret.LocalSecretsProvider@5fb8dc01] - activable => nextflow.secret.LocalSecretsProvider@5fb8dc01 +Feb-02 16:19:34.154 [main] DEBUG nextflow.Session - Session UUID: cb8f7bd7-1f75-4a72-98f5-c98f122e5c9a +Feb-02 16:19:34.155 [main] DEBUG nextflow.Session - Run name: pedantic_gilbert +Feb-02 16:19:34.156 [main] DEBUG nextflow.Session - Executor pool size: 8 +Feb-02 16:19:34.167 [main] DEBUG nextflow.file.FilePorter - File porter settings maxRetries=3; maxTransfers=50; pollTimeout=null +Feb-02 16:19:34.175 [main] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'FileTransfer' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:19:34.202 [main] DEBUG nextflow.cli.CmdRun - + Version: 23.10.1 build 5891 + Created: 12-01-2024 22:01 UTC (22:01 GMT) + System: Linux 5.15.133.1-microsoft-standard-WSL2 + Runtime: Groovy 3.0.19 on OpenJDK 64-Bit Server VM 17.0.10-internal+0-adhoc..src + Encoding: UTF-8 (UTF-8) + Process: 16335@b23599a75663 [172.17.0.3] + CPUs: 8 - Mem: 7.6 GB (947.5 MB) - Swap: 2 GB (2 GB) +Feb-02 16:19:34.233 [main] DEBUG nextflow.Session - Work-dir: /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work [ext2/ext3] +Feb-02 16:19:34.251 [main] DEBUG nextflow.executor.ExecutorFactory - Extension executors providers=[] +Feb-02 16:19:34.265 [main] DEBUG nextflow.Session - Observer factory: DefaultObserverFactory +Feb-02 16:19:34.345 [main] DEBUG nextflow.cache.CacheFactory - Using Nextflow cache factory: nextflow.cache.DefaultCacheFactory +Feb-02 16:19:34.359 [main] DEBUG nextflow.util.CustomThreadPool - Creating default thread pool > poolSize: 9; maxThreads: 1000 +Feb-02 16:19:34.461 [main] DEBUG nextflow.Session - Session start +Feb-02 16:19:34.466 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow started -- trace file: /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/trace.csv +Feb-02 16:19:34.471 [main] DEBUG nextflow.Session - Using default localLib path: /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib +Feb-02 16:19:34.475 [main] DEBUG nextflow.Session - Adding to the classpath library: /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/lib +Feb-02 16:19:34.781 [main] DEBUG nextflow.script.ScriptRunner - > Launching execution +Feb-02 16:19:35.074 [main] DEBUG nextflow.plugin.PluginUpdater - Installing plugin nf-amazon version: 2.1.4 +Feb-02 16:19:35.082 [main] INFO org.pf4j.AbstractPluginManager - Plugin 'nf-amazon@2.1.4' resolved +Feb-02 16:19:35.082 [main] INFO org.pf4j.AbstractPluginManager - Start plugin 'nf-amazon@2.1.4' +Feb-02 16:19:35.100 [main] DEBUG nextflow.plugin.BasePlugin - Plugin started nf-amazon@2.1.4 +Feb-02 16:19:35.117 [main] DEBUG nextflow.file.FileHelper - > Added 'S3FileSystemProvider' to list of installed providers [s3] +Feb-02 16:19:35.117 [main] DEBUG nextflow.file.FileHelper - Started plugin 'nf-amazon' required to handle file: s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_1.fastq.gz +Feb-02 16:19:35.124 [main] DEBUG nextflow.file.FileHelper - Creating a file system instance for provider: S3FileSystemProvider +Feb-02 16:19:35.136 [main] DEBUG nextflow.cloud.aws.config.AwsConfig - AWS S3 config properties: {max_error_retry=5} +Feb-02 16:19:35.659 [main] DEBUG nextflow.cloud.aws.nio.S3Client - Setting S3 glacierRetrievalTier=null +Feb-02 16:19:35.732 [main] DEBUG n.c.aws.util.S3CredentialsProvider - No AWS credentials available - falling back to anonymous access +Feb-02 16:19:37.859 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withLabel:process_medium` matches labels `process_medium` for process with name FASTQC +Feb-02 16:19:37.861 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:FASTQC` matches process FASTQC +Feb-02 16:19:37.863 [main] DEBUG nextflow.script.ProcessConfig - Config settings `withName:.*` matches process FASTQC +Feb-02 16:19:37.873 [main] DEBUG nextflow.executor.ExecutorFactory - << taskConfig executor: null +Feb-02 16:19:37.874 [main] DEBUG nextflow.executor.ExecutorFactory - >> processorType: 'local' +Feb-02 16:19:37.882 [main] DEBUG nextflow.executor.Executor - [warm up] executor > local +Feb-02 16:19:37.887 [main] DEBUG n.processor.LocalPollingMonitor - Creating local task monitor for executor 'local' > cpus=8; memory=7.6 GB; capacity=8; pollInterval=100ms; dumpInterval=5m +Feb-02 16:19:37.890 [main] DEBUG n.processor.TaskPollingMonitor - >>> barrier register (monitor: local) +Feb-02 16:19:37.992 [main] DEBUG nextflow.Session - Config process names validation disabled as requested +Feb-02 16:19:37.993 [main] DEBUG nextflow.Session - Igniting dataflow network (2) +Feb-02 16:19:37.993 [main] DEBUG nextflow.processor.TaskProcessor - Starting process > FASTQC +Feb-02 16:19:37.995 [main] DEBUG nextflow.script.ScriptRunner - Parsed script files: + Script_72d0a310756a06d3: /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/mock.nf + Script_4ba798786ecd6418: /workspaces/readsimulator/./modules/nf-core/fastqc/tests/../main.nf +Feb-02 16:19:37.995 [main] DEBUG nextflow.script.ScriptRunner - > Awaiting termination +Feb-02 16:19:37.995 [main] DEBUG nextflow.Session - Session await +Feb-02 16:19:38.495 [FileTransfer-1] DEBUG nextflow.file.FilePorter - Copying foreign file s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_1.fastq.gz to work dir: /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/stage-cb8f7bd7-1f75-4a72-98f5-c98f122e5c9a/19/e96dfce5e60dd6d781182ee5723e5f/test_1.fastq.gz +Feb-02 16:19:38.496 [FileTransfer-2] DEBUG nextflow.file.FilePorter - Copying foreign file s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_2.fastq.gz to work dir: /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/stage-cb8f7bd7-1f75-4a72-98f5-c98f122e5c9a/d0/c320a707efa7b1e565f4446bcf02f0/test_2.fastq.gz +Feb-02 16:19:38.695 [FileTransfer-2] DEBUG n.cloud.aws.nio.S3FileSystemProvider - S3 download file from=s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_2.fastq.gz to=/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/stage-cb8f7bd7-1f75-4a72-98f5-c98f122e5c9a/d0/c320a707efa7b1e565f4446bcf02f0/test_2.fastq.gz +Feb-02 16:19:38.696 [FileTransfer-2] DEBUG nextflow.cloud.aws.nio.S3Client - Creating S3 transfer manager pool - chunk-size=104857600; max-treads=10; +Feb-02 16:19:38.697 [FileTransfer-2] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'S3TransferManager' minSize=10; maxSize=10; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:19:39.354 [FileTransfer-1] DEBUG n.cloud.aws.nio.S3FileSystemProvider - S3 download file from=s3://ngi-igenomes/testdata/nf-core/modules/genomics/sarscov2/illumina/fastq/test_1.fastq.gz to=/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/stage-cb8f7bd7-1f75-4a72-98f5-c98f122e5c9a/19/e96dfce5e60dd6d781182ee5723e5f/test_1.fastq.gz +Feb-02 16:19:39.988 [Actor Thread 7] WARN nextflow.container.SingularityCache - Singularity cache directory has not been defined -- Remote image will be stored in the path: /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/singularity -- Use the environment variable NXF_SINGULARITY_CACHEDIR to specify a different location +Feb-02 16:19:39.989 [Actor Thread 7] INFO nextflow.container.SingularityCache - Pulling Singularity image https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0 [cache /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/singularity/depot.galaxyproject.org-singularity-fastqc-0.12.1--hdfd78af_0.img] +Feb-02 16:19:40.751 [Actor Thread 7] DEBUG nextflow.container.SingularityCache - Singularity pull complete image=https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0 path=/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/singularity/depot.galaxyproject.org-singularity-fastqc-0.12.1--hdfd78af_0.img +Feb-02 16:19:40.871 [Task submitter] DEBUG n.executor.local.LocalTaskHandler - Launch cmd line: /bin/bash -ue .command.run +Feb-02 16:19:40.874 [Task submitter] INFO nextflow.Session - [dc/7148c1] Submitted process > FASTQC (test) +Feb-02 16:19:45.077 [Task monitor] DEBUG n.processor.TaskPollingMonitor - Task completed > TaskHandler[id: 1; name: FASTQC (test); status: COMPLETED; exit: 0; error: -; workDir: /workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/dc/7148c103601d4fa1b80fe4c2434f96] +Feb-02 16:19:45.103 [Task monitor] WARN nextflow.processor.PublishDir - Process `FASTQC` publishDir path contains a variable with a null value +Feb-02 16:19:45.106 [Task monitor] DEBUG nextflow.util.ThreadPoolBuilder - Creating thread pool 'PublishDir' minSize=10; maxSize=24; workQueue=LinkedBlockingQueue[10000]; allowCoreThreadTimeout=false +Feb-02 16:19:45.113 [main] DEBUG nextflow.Session - Session await > all processes finished +Feb-02 16:19:45.117 [Task monitor] DEBUG n.processor.TaskPollingMonitor - <<< barrier arrives (monitor: local) - terminating tasks monitor poll loop +Feb-02 16:19:45.117 [main] DEBUG nextflow.Session - Session await > all barriers passed +Feb-02 16:19:45.120 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'PublishDir' shutdown completed (hard=false) +Feb-02 16:19:45.129 [main] DEBUG n.trace.WorkflowStatsObserver - Workflow completed > WorkflowStats[succeededCount=1; failedCount=0; ignoredCount=0; cachedCount=0; pendingCount=0; submittedCount=0; runningCount=0; retriesCount=0; abortedCount=0; succeedDuration=8s; failedDuration=0ms; cachedDuration=0ms;loadCpus=0; loadMemory=0; peakRunning=1; peakCpus=2; peakMemory=3 GB; ] +Feb-02 16:19:45.130 [main] DEBUG nextflow.trace.TraceFileObserver - Workflow completed -- saving trace file +Feb-02 16:19:45.181 [main] DEBUG nextflow.cache.CacheDB - Closing CacheDB done +Feb-02 16:19:45.181 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-amazon@2.1.4' +Feb-02 16:19:45.181 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-amazon +Feb-02 16:19:45.181 [main] INFO org.pf4j.AbstractPluginManager - Stop plugin 'nf-validation@1.1.3' +Feb-02 16:19:45.181 [main] DEBUG nextflow.plugin.BasePlugin - Plugin stopped nf-validation +Feb-02 16:19:45.200 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'FileTransfer' shutdown completed (hard=false) +Feb-02 16:19:45.201 [main] DEBUG nextflow.util.ThreadPoolManager - Thread pool 'S3TransferManager' shutdown completed (hard=false) +Feb-02 16:19:45.201 [main] DEBUG nextflow.script.ScriptRunner - > Execution complete -- Goodbye diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_0.json b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_0.json new file mode 100644 index 0000000..c160279 --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_0.json @@ -0,0 +1 @@ +{"0":[[{"id":"test","single_end":false},["/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/dc/7148c103601d4fa1b80fe4c2434f96/test_1_fastqc.html","/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/dc/7148c103601d4fa1b80fe4c2434f96/test_2_fastqc.html"]]]} \ No newline at end of file diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_1.json b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_1.json new file mode 100644 index 0000000..bc3c32c --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_1.json @@ -0,0 +1 @@ +{"1":[[{"id":"test","single_end":false},["/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/dc/7148c103601d4fa1b80fe4c2434f96/test_1_fastqc.zip","/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/dc/7148c103601d4fa1b80fe4c2434f96/test_2_fastqc.zip"]]]} \ No newline at end of file diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_2.json b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_2.json new file mode 100644 index 0000000..79723e7 --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_2.json @@ -0,0 +1 @@ +{"2":["/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/dc/7148c103601d4fa1b80fe4c2434f96/versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_html.json b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_html.json new file mode 100644 index 0000000..104eb7e --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_html.json @@ -0,0 +1 @@ +{"html":[[{"id":"test","single_end":false},["/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/dc/7148c103601d4fa1b80fe4c2434f96/test_1_fastqc.html","/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/dc/7148c103601d4fa1b80fe4c2434f96/test_2_fastqc.html"]]]} \ No newline at end of file diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_versions.json b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_versions.json new file mode 100644 index 0000000..423d8b3 --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_versions.json @@ -0,0 +1 @@ +{"versions":["/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/dc/7148c103601d4fa1b80fe4c2434f96/versions.yml"]} \ No newline at end of file diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_zip.json b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_zip.json new file mode 100644 index 0000000..8156b9f --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/output_zip.json @@ -0,0 +1 @@ +{"zip":[[{"id":"test","single_end":false},["/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/dc/7148c103601d4fa1b80fe4c2434f96/test_1_fastqc.zip","/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/work/dc/7148c103601d4fa1b80fe4c2434f96/test_2_fastqc.zip"]]]} \ No newline at end of file diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/params.json b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/params.json new file mode 100644 index 0000000..aa743ff --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/params.json @@ -0,0 +1 @@ +{"nf_test_output":"/workspaces/readsimulator/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta"} \ No newline at end of file diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/std.err b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/std.err new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/std.out b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/std.out new file mode 100644 index 0000000..e69de29 diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/trace.csv b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/trace.csv new file mode 100644 index 0000000..bd83133 --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/trace.csv @@ -0,0 +1,2 @@ +task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar +1 dc/7148c1 16533 FASTQC (test) COMPLETED 0 2024-02-02 16:19:40.874 4.2s 4s 167.9% 375.1 MB 4 GB 12.3 MB 3.9 MB diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/workflow.json b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/workflow.json new file mode 100644 index 0000000..7d54df0 --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/meta/workflow.json @@ -0,0 +1 @@ +{"success":true,"exitStatus":0} \ No newline at end of file diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/null/fastqc/test_1_fastqc.html b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/null/fastqc/test_1_fastqc.html new file mode 100644 index 0000000..e72abac --- /dev/null +++ b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/null/fastqc/test_1_fastqc.html @@ -0,0 +1,187 @@ +test_1.gz FastQC Report
    FastQCFastQC Report
    Fri 2 Feb 2024
    test_1.gz

    Summary

    [OK]Basic Statistics

    MeasureValue
    Filenametest_1.gz
    File typeConventional base calls
    EncodingSanger / Illumina 1.9
    Total Sequences100
    Total Bases13.8 kbp
    Sequences flagged as poor quality0
    Sequence length72-151
    %GC39

    [OK]Per base sequence quality

    Per base quality graph

    [FAIL]Per tile sequence quality

    Per tile quality graph

    [OK]Per sequence quality scores

    Per Sequence quality graph

    [FAIL]Per base sequence content

    Per base sequence content

    [FAIL]Per sequence GC content

    Per sequence GC content graph

    [OK]Per base N content

    N content graph

    [WARN]Sequence Length Distribution

    Sequence length distribution

    [OK]Sequence Duplication Levels

    Duplication level graph

    [FAIL]Overrepresented sequences

    SequenceCountPercentagePossible Source
    CAACTACACTTAACGGTCTTTGGCTTGATGACGTAGTTTACTGTCCAAGA22.0No Hit
    ACTCTAGATAATTCATTAGGTTTCTTAATAGTAAGACTAGAATTGTCTAC11.0No Hit
    GTCTAATCATAATTTCTTGGTACAGGCTGGTATTGTTCATCTCAGGGTTA11.0No Hit
    TGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGACACTTA11.0No Hit
    TTTCAAGACTTGTAAATTGCAACTTGTCATAAAGGTCTCTATCAGACATT11.0No Hit
    TTACCAATGTGCTATGAGGCCCAATTTCACTATTAAGGGTTCATTCCTTA11.0No Hit
    ATGTGAGAAGGCATTAAAATATTTGCCTATAGATAAATGTAGTAGAATTA11.0No Hit
    GTTCTTACCTTTCTTTTCCAATGTTACTTGGTTCCATGCTATACATGTCT11.0No Hit
    AAGTGTATCTTGATCCTCATAACTCATTGAATCATAATAAAGTCTAGCCT11.0No Hit
    GTCAACCAAAATGCACAAGCTTTAAACACGCTTGTTAAACAACTTAGCTC11.0No Hit
    AATCAAGACTATTCAACCAAGGGTTGAAAAGAAAAAGCTTGATGGCTTTA11.0No Hit
    CAATTTGCCTATGCCAACAGGAATAGGTTTTTGTATATAATTAAGTTAAT11.0No Hit
    GTTCCTAGGTAGTAGAAATACCATCTAGGACTGAGATCTTTCATTTTACA11.0No Hit
    CATCTTATTTCTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGA11.0No Hit
    TATCTGCCCATTTAATAGAAGTTAAACCATTAACTTGTGGGTATTTCCAC11.0No Hit
    CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT11.0No Hit
    ACTGAATTTTCTGCACCAAGTGACATAGTGTAGGCAATGATGGATTGACT11.0No Hit
    AACTAGCATTATACACTGAAGTGTATTACCAGTTATGAAGAAAATAGGGC11.0No Hit
    AAGTTGGCGTATACGCGTAATATATCTGGGTTTTCTACAAAATCATACCA11.0No Hit
    TTCCAGAAGAGCAAGGTTCTTTTAAAAGTACTGTTGTACCTCTAACACAC11.0No Hit
    TATGTAGCCATACTCCACTCATCTAAATCAATGCCCAGTGGTGTAAGTAA11.0No Hit
    TTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTAATGGGTAA11.0No Hit
    GGTGTATTCAAGGCTCCCTCAGTTGCAACCCATATGATGCCGTCTTTGTT11.0No Hit
    GTCTACAAGCTGGTAATGCAACAGAAGTGCCTGCCAATTCAACTGTATTA11.0No Hit
    AAAGGAGCTGGTGGCCATAGTTACGGCGCCGATCTAAAGTCATTTGACTT11.0No Hit
    ACATAGTGTAGGCAATGATGGATTGACTAGCTACACTACGTGCCCGCCGA11.0No Hit
    GTTTGTGGACCTAAAAAGTCTACTAATTTGGTTAAAAACAAATGTGTCAA11.0No Hit
    CAGGTTATGTTGATACACCTAATAATACAGATTTTTCCAGAGTTAGTGCT11.0No Hit
    GGATAGACAGATCGAATTCTACCCATAAAGCCATCAAGCTTTTTCTTTTC11.0No Hit
    ACTGTTTTCTTTGTAGAAAACATCCGTAATAGGACCTTTGTATTCTGAGG11.0No Hit
    GATGAAACATCATGCGTGATAACACCCTTATAAAACATTTTAAAGCATTG11.0No Hit
    GTTATAACACTGACACCACCAAAAGAACATGGTGTAATGTCAAGAATCTC11.0No Hit
    GCTCCATATATAGTGGGTGATGTTGTTCAAGAGGGTGTTTTAACTGCTGT11.0No Hit
    CCTGTTGTCTATGTGATAGACGTGCCACATGCTTTTCCACTGCTTCAGAC11.0No Hit
    CAGTAGTCTTACTCTCAGTTTTGCAACAACTCAGAGTAGAATCATCATCT11.0No Hit
    CATTAGCACAAGTTGTAGGTATTTGTACATACTTACCTTTTAAGTCACAA11.0No Hit
    GTTACTCACAATTTTGACTTCACTTTTAGTCTTAGTCCAGAGTACTCAAT11.0No Hit
    TGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGTGGTGGTG11.0No Hit
    CTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGCCGCTGTTG11.0No Hit
    TCTTTGTAAGCTTTAGCAGCATCTACAGCAAAAGCACAGAAAGATAATAC11.0No Hit
    ATGTCAGATTTATGTGACAATTTAAGACATTCAACAACATCTTTAGTTTC11.0No Hit
    GGATTAGTAACACTACAGCTGATGCATACATAACACAGTCTTTTAGCTTA11.0No Hit
    CTGTAATAGTTGTGTCCGTACCAGCTGCTTGTGCTGTTTGCCTGTCAACA11.0No Hit
    GAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGCACCTG11.0No Hit
    CTTTTACAAGACTTCAGAGTTTAGAAATTGTGGCTTATAATGTTGTAATT11.0No Hit
    TGGCAATAGAGTTATTAGAGTAAGCAACTGAATTTTCTGCACCAAGTGAC11.0No Hit
    CACAAACTCTAAAAGAATGTATAGGGTCAGCACCAAAAATACCAGCAGAT11.0No Hit
    CCTTATGACAGCAAGAACTGTGTATGATGATGGTGCTAGGAGAGTGTGGA11.0No Hit
    AACACCAGCTTCTGATCTTTCACAAGTGCCGTGCCTACAGTACTCAGAAT11.0No Hit
    GATTTTTAAGTGTGTCACTTAACATTTGTACAATCTTTATACGCACTACA11.0No Hit
    AGAGTAGACTATATATCGTAAACGGAAAAGCGAAAACGTTTATATAGCCC11.0No Hit
    AGTTGCAATTTACAAGTCTTGAAATTCCACGTAGGAATGTGGCAACTTTA11.0No Hit
    CATGGGCACACTTTCTTATGAACAATTTAAGAAAGGTGTTCAGATACCTT11.0No Hit
    GTACATAACAGCTTCTACACCCTTAAGGGTTGTCTGCTGTTGTCCACAAG11.0No Hit
    ACCTGATGCTGTTACAGCGTATAATGGTTATCTTACTTCTTCTTCTAAAA11.0No Hit
    CACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATA11.0No Hit
    TGCCAATCCTGTAGCGACTGTATGCAGCAAAACCTGAGTCACCTGCTACA11.0No Hit
    GATAAACGCACTACGTGCTTTTCAGTAGCTGCACTTACTAACAATGTTGC11.0No Hit
    GTACGAACTTAAGCATGGTACATTTACTTGTGCTAGTGAGTACACTGGTA11.0No Hit
    CTATAACATTAATAGGCAATGAACCTTTAGTGTTATTAGCTCTCAGGTTG11.0No Hit
    ATAAACATGTTAGGGTAACCATTAACTTGATAATTCATTTTAAAACCCAT11.0No Hit
    CCAAGCAGGGTTACGTGTAAGGAATTCTCTTACCACGCCTATTTGTGGCC11.0No Hit
    GATTTAGCCACATTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGA11.0No Hit
    GTTGAAAGTAGATCTTCAATAAATGACCTCTTGCTTGGTTTTGATGGATC11.0No Hit
    TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCG11.0No Hit
    TAATGACCCTGTGGGTTTTACACTTAAAAACACAGTCTGTACCGTCTGCG11.0No Hit
    GCTATAACGCAGCCTGTAAAATCATCTGGTAATTTATAATTATAATCAGC11.0No Hit
    AGTATGGACAATTCACCTAATTTAGCATGGCCTCTTATTGTAACAGCTTT11.0No Hit
    TCTAAGCATAGTGAAAAGCATTGTCTGCATAGCACTAGTAACTTTTGCCC11.0No Hit
    GAAGAAGATTGGTTAGATGATGATAGTCAACAAACTGTTGGTCAACAAGA11.0No Hit
    ATGATGAACCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGCTGAT11.0No Hit
    CCGTCTATGCAATACAAAGTTTCTTTAGAAGTTATATGTTTATAGTGACC11.0No Hit
    GCAATTCATAGCTCTTTTCAGAACGTTCCGTGTACCAAGCAATTTCATGC11.0No Hit
    AGTAAACTGTTACAAACAACAACAGCAAGTTGCAAACAAAGTGAACACCC11.0No Hit
    GTACGAACTTATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAG11.0No Hit
    GTGCTCAAAGGAGTCAAATTACATTACACATAAACGAACTTATGGATTTG11.0No Hit
    CTCCAAAGCCACGTACGAGCACGTCGCGAACCTGTAAAACAGGCAAACTG11.0No Hit
    GTTTATGATTGATGTTCAACAATGGGGTTTTACAGGTAACCTACAAAGCA11.0No Hit
    GTGTAACCATTTAAACCCTGACCCGGGTAAGTGGTTATATAATTGTCTGT11.0No Hit
    GTACAAAAATAGCCTAAGAAACAATAAACTAGCATTATACACTGAAGTGT11.0No Hit
    GATTTTTCCAGAGTTAGTGCTAAACCACCGCCTGGAGATCAATTTAAACA11.0No Hit
    GTGAAATTAGTATGGACAAATCACCTAATTTAGCATGGCCTCTTATTGTA11.0No Hit
    TCAATGGGGAATGTCCAAATTTTGTATTTCCCTTAAATTCCATAATCAAG11.0No Hit
    GCTTTGAGTTGACATCTATGAAGTATTTTGTGAAAATAGGACCTGAGCGC11.0No Hit
    TGCGTCTTGGGTCCCCGCTCTCACTCAACATGGCAAGGAAGACCTTAAAT11.0No Hit
    AACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCA11.0No Hit
    CTAAAAGTGAAGTCAAAATTGTGAGTAACAACCAGTGGTGTGTACCCGTG11.0No Hit
    ATGTAAGGCTTTGTTAAGTCAGTGTCAACATGTGACTCTGCAGTTAAAGC11.0No Hit
    CCTTGAATGAGTCTAATTCAGGTTGCAAAGGATCATAAACTGTGTTGTTG11.0No Hit
    GCTCTCTACTACCCTTCTGCTCGCATAGTGTATACAGCTTGCTCTCATGC11.0No Hit
    ATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGG11.0No Hit
    GCTGCTAGAGACCTCGTTTGTGCACAAAAGTTTAACGGCCTTACTGTTTT11.0No Hit
    CCCTTTGTTGTGTTGTAGTAAGCTAACGCATTGTCATCAGTGCAAGCAGT11.0No Hit
    ATCCTTTAGGATTTGGATGATCTATGTGGCAACGGCAGTACAGACAACAC11.0No Hit
    CTACACGAGCACGTGCAGGTATAATTCTACTACATTTATCTATAGGCAAA11.0No Hit
    TGCCTGACCAGTACCAGTGTGTGTACACAACATCTTAACACAATTAGTGA11.0No Hit
    GTCTATATGCCTGCTAGTTGGGTGATGCGTATTATGACATGGTTGGATAT11.0No Hit
    ATATTACGCGTATACGCCAACTTAGGTGAACGTGTACGCCAAGCTTTGTT11.0No Hit
    TTACCTGCACCACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATA11.0No Hit

    [OK]Adapter Content

    Adapter graph

    Produced by FastQC (version 0.12.1)
    \ No newline at end of file diff --git a/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/null/fastqc/test_1_fastqc.zip b/.nf-test/tests/4f4b9d075edbb7cb770c2609ebfb82cb/null/fastqc/test_1_fastqc.zip new file mode 100644 index 0000000000000000000000000000000000000000..86c88384104d411ba053ae25a71eba64348e8018 GIT binary patch literal 454620 zcmce-1C(UV)-77L(Pg{3Y<1byW!tuG+f`k*ZQHhO+kW+Z=lpk^A7_kr-@D`H&d8lR zV#XRPRz%JjJ9eau1TY8+00aaCfP){u3c$a-;J&{*7}`5%(`y^)+B?|l(*gs?NPt89 z1p)dePUhd%M)_~G#q_PM?EklHp#FComb%7<|9eJ={=1RC9r;I_v`xP+Xl$&E0V_b{ z-a=)hL|~yXp}woYiirx!eV_6FN)TY*pZ0EU_unTFdwwy6?|4CYKp2F4Uqjl6s@i|! zm;OqC{N!ma-rdH--al~1o;$H z0xOaCBk6&q95!>}0+x*zN zyUB8&`t=l2T8namR5HmI-YeN1*bUmvWCMl{#tjZewQv*oKCB11Oz7CHwN0R-i1hxLxB13<26U?VLBoJPH{ z^{zu4Vb~-J^qkFmt>t?`B7+wQ=KJPr4dZ6+BM=gT2jd808p{$0hs+B7h+v}^-tY6| zqwWU=)YMS+7)+`YF6a~S2I}>)=vSy?leK}Fz9#cS<#kvtgGlKfg3u_uSq=G&7%i$r*Z`6<*G5Xu?q3%4ua;`m(dHjH7KxXfc1kfUW zgy#VeDB=!RNT1S7&`BU7K122Vp=~1lRj*h9S#-0Y73ywOdbKk(SAr=O-3KlpA!6h1 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    Summary

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    Fasta
    Fasta
    Fasta
    Fasta
    Wgsim
    Wgsim
    InSilicoSeq
    InSilicoSeq
    Whole genome
    Whole genome
    Metagenome
    Metagenome
    Amplicon
    Amplicon
    Target capture
    Target capture
    CRABS
    CRABS
    ART
    ART
    Bowtie2
    Bowtie2
    Samtools
    Samtools
    CapSim
    CapSim
    Create Samplesheet
    Create Sam...
    Fastqc
    Fastqc
    Multiqc
    Multiqc
    Legend
    Legend
    Reference
    Reference
    Probes
    Probes
    Simulated Reads
    Simulated Reads
    Start
    Start
    Step
    Step
    nf-core/readsimulator
    nf-core/readsimulator
    1.0.0
    1.0.0    Text is not SVG - cannot display     \ No newline at end of file From de73299c5ad2ec26ed0eccf0817317bb337e3c11 Mon Sep 17 00:00:00 2001 From: Adam Bennett Date: Thu, 23 Nov 2023 00:49:28 +0000 Subject: [PATCH 49/89] Added environment.yml for local modules --- modules/local/crabs/dbdownload/environment.yml | 7 +++++++ .../{crabs_dbdownload.nf => crabs/dbdownload/main.nf} | 2 +- modules/local/crabs/dbimport/environment.yml | 7 +++++++ .../local/{crabs_dbimport.nf => crabs/dbimport/main.nf} | 2 +- modules/local/crabs/insilicopcr/environment.yml | 7 +++++++ .../{crabs_insilicopcr.nf => crabs/insilicopcr/main.nf} | 2 +- .../create_samplesheet/main.nf} | 0 modules/local/custom/merge_samplesheets/environment.yml | 7 +++++++ .../merge_samplesheets/main.nf} | 2 +- modules/local/insilicoseq/generate/environment.yml | 7 +++++++ .../generate/main.nf} | 2 +- modules/local/{japsa_capsim.nf => japsa/capsim/main.nf} | 0 modules/local/unzip/environment.yml | 7 +++++++ modules/local/{unzip.nf => unzip/main.nf} | 4 +--- modules/local/wgsim/environment.yml | 7 +++++++ modules/local/{wgsim.nf => wgsim/main.nf} | 2 +- subworkflows/local/amplicon_workflow.nf | 6 +++--- subworkflows/local/target_capture_workflow.nf | 4 ++-- workflows/readsimulator.nf | 8 ++++---- 19 files changed, 65 insertions(+), 18 deletions(-) create mode 100644 modules/local/crabs/dbdownload/environment.yml rename modules/local/{crabs_dbdownload.nf => crabs/dbdownload/main.nf} (95%) create mode 100644 modules/local/crabs/dbimport/environment.yml rename modules/local/{crabs_dbimport.nf => crabs/dbimport/main.nf} (97%) create mode 100644 modules/local/crabs/insilicopcr/environment.yml rename modules/local/{crabs_insilicopcr.nf => crabs/insilicopcr/main.nf} (96%) rename modules/local/{create_samplesheet.nf => custom/create_samplesheet/main.nf} (100%) create mode 100644 modules/local/custom/merge_samplesheets/environment.yml rename modules/local/{merge_samplesheets.nf => custom/merge_samplesheets/main.nf} (95%) create mode 100644 modules/local/insilicoseq/generate/environment.yml rename modules/local/{insilicoseq_generate.nf => insilicoseq/generate/main.nf} (98%) rename modules/local/{japsa_capsim.nf => japsa/capsim/main.nf} (100%) create mode 100644 modules/local/unzip/environment.yml rename modules/local/{unzip.nf => unzip/main.nf} (84%) create mode 100644 modules/local/wgsim/environment.yml rename modules/local/{wgsim.nf => wgsim/main.nf} (97%) diff --git a/modules/local/crabs/dbdownload/environment.yml b/modules/local/crabs/dbdownload/environment.yml new file mode 100644 index 0000000..8380980 --- /dev/null +++ b/modules/local/crabs/dbdownload/environment.yml @@ -0,0 +1,7 @@ +name: crabs_dbdownload +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::crabs=0.1.1-0 diff --git a/modules/local/crabs_dbdownload.nf b/modules/local/crabs/dbdownload/main.nf similarity index 95% rename from modules/local/crabs_dbdownload.nf rename to modules/local/crabs/dbdownload/main.nf index 4025182..f3a9331 100644 --- a/modules/local/crabs_dbdownload.nf +++ b/modules/local/crabs/dbdownload/main.nf @@ -4,7 +4,7 @@ process CRABS_DBDOWNLOAD { label 'process_medium' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. - conda "bioconda::crabs=0.1.1-0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/crabs:0.1.1--pyhb7b1952_0': 'biocontainers/crabs:0.1.1--pyhb7b1952_0' }" diff --git a/modules/local/crabs/dbimport/environment.yml b/modules/local/crabs/dbimport/environment.yml new file mode 100644 index 0000000..b4f8928 --- /dev/null +++ b/modules/local/crabs/dbimport/environment.yml @@ -0,0 +1,7 @@ +name: crabs_dbimport +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::crabs=0.1.1-0 diff --git a/modules/local/crabs_dbimport.nf b/modules/local/crabs/dbimport/main.nf similarity index 97% rename from modules/local/crabs_dbimport.nf rename to modules/local/crabs/dbimport/main.nf index 67f6b97..e67a13b 100644 --- a/modules/local/crabs_dbimport.nf +++ b/modules/local/crabs/dbimport/main.nf @@ -3,7 +3,7 @@ process CRABS_DBIMPORT { label 'process_medium' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. - conda "bioconda::crabs=0.1.1-0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/crabs:0.1.1--pyhb7b1952_0': 'biocontainers/crabs:0.1.1--pyhb7b1952_0' }" diff --git a/modules/local/crabs/insilicopcr/environment.yml b/modules/local/crabs/insilicopcr/environment.yml new file mode 100644 index 0000000..d90cc41 --- /dev/null +++ b/modules/local/crabs/insilicopcr/environment.yml @@ -0,0 +1,7 @@ +name: crabs_insilicopcr +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::crabs=0.1.1-0 diff --git a/modules/local/crabs_insilicopcr.nf b/modules/local/crabs/insilicopcr/main.nf similarity index 96% rename from modules/local/crabs_insilicopcr.nf rename to modules/local/crabs/insilicopcr/main.nf index 3f1efe9..de4c452 100644 --- a/modules/local/crabs_insilicopcr.nf +++ b/modules/local/crabs/insilicopcr/main.nf @@ -3,7 +3,7 @@ process CRABS_INSILICOPCR { label 'process_medium' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. - conda "bioconda::crabs=0.1.1-0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/crabs:0.1.1--pyhb7b1952_0': 'biocontainers/crabs:0.1.1--pyhb7b1952_0' }" diff --git a/modules/local/create_samplesheet.nf b/modules/local/custom/create_samplesheet/main.nf similarity index 100% rename from modules/local/create_samplesheet.nf rename to modules/local/custom/create_samplesheet/main.nf diff --git a/modules/local/custom/merge_samplesheets/environment.yml b/modules/local/custom/merge_samplesheets/environment.yml new file mode 100644 index 0000000..c7eedba --- /dev/null +++ b/modules/local/custom/merge_samplesheets/environment.yml @@ -0,0 +1,7 @@ +name: merge_samplesheet +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - conda-forge::sed=4.7 diff --git a/modules/local/merge_samplesheets.nf b/modules/local/custom/merge_samplesheets/main.nf similarity index 95% rename from modules/local/merge_samplesheets.nf rename to modules/local/custom/merge_samplesheets/main.nf index af29c32..d7ae1d9 100644 --- a/modules/local/merge_samplesheets.nf +++ b/modules/local/custom/merge_samplesheets/main.nf @@ -2,7 +2,7 @@ process MERGE_SAMPLESHEETS { tag "$meta.id" label 'process_single' - conda "conda-forge::sed=4.7" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : 'nf-core/ubuntu:20.04' }" diff --git a/modules/local/insilicoseq/generate/environment.yml b/modules/local/insilicoseq/generate/environment.yml new file mode 100644 index 0000000..e706c95 --- /dev/null +++ b/modules/local/insilicoseq/generate/environment.yml @@ -0,0 +1,7 @@ +name: insilicoseq_generate +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::insilicoseq=1.6.0 diff --git a/modules/local/insilicoseq_generate.nf b/modules/local/insilicoseq/generate/main.nf similarity index 98% rename from modules/local/insilicoseq_generate.nf rename to modules/local/insilicoseq/generate/main.nf index f37e387..dbb1b41 100644 --- a/modules/local/insilicoseq_generate.nf +++ b/modules/local/insilicoseq/generate/main.nf @@ -2,7 +2,7 @@ process INSILICOSEQ_GENERATE { tag "$meta.id" label 'process_low' - conda "bioconda::insilicoseq=1.6.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/insilicoseq:1.6.0--pyh7cba7a3_0': 'biocontainers/insilicoseq:1.6.0--pyh7cba7a3_0' }" diff --git a/modules/local/japsa_capsim.nf b/modules/local/japsa/capsim/main.nf similarity index 100% rename from modules/local/japsa_capsim.nf rename to modules/local/japsa/capsim/main.nf diff --git a/modules/local/unzip/environment.yml b/modules/local/unzip/environment.yml new file mode 100644 index 0000000..0c6171c --- /dev/null +++ b/modules/local/unzip/environment.yml @@ -0,0 +1,7 @@ +name: unzip +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - conda-forge::unzip=6.0 diff --git a/modules/local/unzip.nf b/modules/local/unzip/main.nf similarity index 84% rename from modules/local/unzip.nf rename to modules/local/unzip/main.nf index 554d8f6..0523e5e 100644 --- a/modules/local/unzip.nf +++ b/modules/local/unzip/main.nf @@ -2,17 +2,15 @@ process UNZIP { tag "$file" label 'process_single' - conda "conda-forge::unzip=6.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/unzip:6.0': 'biocontainers/unzip:6.0' }" input: path(file) - //tuple val(meta), path(file) output: - //tuple val(meta), path "unziped/*", emit: file path "unziped/*" , emit: file path "versions.yml", emit: versions diff --git a/modules/local/wgsim/environment.yml b/modules/local/wgsim/environment.yml new file mode 100644 index 0000000..0f45674 --- /dev/null +++ b/modules/local/wgsim/environment.yml @@ -0,0 +1,7 @@ +name: wgsim +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::wgsim=1.0 diff --git a/modules/local/wgsim.nf b/modules/local/wgsim/main.nf similarity index 97% rename from modules/local/wgsim.nf rename to modules/local/wgsim/main.nf index 8a3790e..e664911 100644 --- a/modules/local/wgsim.nf +++ b/modules/local/wgsim/main.nf @@ -3,7 +3,7 @@ process WGSIM { label 'process_single' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. - conda "bioconda::wgsim=1.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/wgsim:1.0--he4a0461_7': 'biocontainers/wgsim:1.0--he4a0461_7' }" diff --git a/subworkflows/local/amplicon_workflow.nf b/subworkflows/local/amplicon_workflow.nf index b109c28..f6612c1 100644 --- a/subworkflows/local/amplicon_workflow.nf +++ b/subworkflows/local/amplicon_workflow.nf @@ -2,9 +2,9 @@ // Simulate amplicon reads // -include { CRABS_DBDOWNLOAD } from '../../modules/local/crabs_dbdownload' -include { CRABS_DBIMPORT } from '../../modules/local/crabs_dbimport' -include { CRABS_INSILICOPCR } from '../../modules/local/crabs_insilicopcr' +include { CRABS_DBDOWNLOAD } from '../../modules/local/crabs/dbdownload/main' +include { CRABS_DBIMPORT } from '../../modules/local/crabs/dbimport/main' +include { CRABS_INSILICOPCR } from '../../modules/local/crabs/insilicopcr/main' include { ART_ILLUMINA } from '../../modules/nf-core/art/illumina/main' workflow AMPLICON_WORKFLOW { diff --git a/subworkflows/local/target_capture_workflow.nf b/subworkflows/local/target_capture_workflow.nf index 448484b..c3d1c0d 100644 --- a/subworkflows/local/target_capture_workflow.nf +++ b/subworkflows/local/target_capture_workflow.nf @@ -5,8 +5,8 @@ include { BOWTIE2_BUILD } from '../../modules/nf-core/bowtie2/build/main' include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/main' include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' -include { JAPSA_CAPSIM } from '../../modules/local/japsa_capsim' -include { UNZIP as UNZIP_PROBE } from '../../modules/local/unzip' +include { JAPSA_CAPSIM } from '../../modules/local/japsa/capsim/main' +include { UNZIP as UNZIP_PROBE } from '../../modules/local/unzip/main' workflow TARGET_CAPTURE_WORKFLOW { take: diff --git a/workflows/readsimulator.nf b/workflows/readsimulator.nf index 133d6d4..ef048b6 100644 --- a/workflows/readsimulator.nf +++ b/workflows/readsimulator.nf @@ -35,10 +35,10 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // MODULE: Local modules // -include { INSILICOSEQ_GENERATE } from '../modules/local/insilicoseq_generate' -include { CREATE_SAMPLESHEET } from '../modules/local/create_samplesheet' -include { MERGE_SAMPLESHEETS } from '../modules/local/merge_samplesheets' -include { WGSIM } from '../modules/local/wgsim' +include { INSILICOSEQ_GENERATE } from '../modules/local/insilicoseq/generate/main' +include { CREATE_SAMPLESHEET } from '../modules/local/custom/create_samplesheet/main' +include { MERGE_SAMPLESHEETS } from '../modules/local/custom/merge_samplesheets/main' +include { WGSIM } from '../modules/local/wgsim/main' // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules From f637cdea50c148964a8d4604f323505e74ccea8b Mon Sep 17 00:00:00 2001 From: Friederike Hanssen Date: Wed, 29 Nov 2023 08:36:38 +0100 Subject: [PATCH 50/89] Update nextflow_schema.json MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index f0cffee..de6ce4f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -117,7 +117,7 @@ }, "amplicon_bold_db": { "type": "string", - "description": "BOLD database to use when `--amplicon_ref_source` is set to 'bold', seperate databases with '|'", + "description": "BOLD database to use when `--amplicon_ref_source` is set to 'bold'. Separate database names with `|`", "default": "Actinopterygii|Aves" }, "amplicon_read_count": { From 8453d885c0ec47849afb4e06d350ad09d6944a2b Mon Sep 17 00:00:00 2001 From: Phil Ewels Date: Thu, 30 Nov 2023 23:44:26 -0500 Subject: [PATCH 51/89] Apply suggestions from code review Co-authored-by: Adam Talbot <12817534+adamrtalbot@users.noreply.github.com> --- README.md | 4 ++-- modules/local/crabs/dbdownload/main.nf | 12 +++++++++++- subworkflows/local/target_capture_workflow.nf | 2 +- 3 files changed, 14 insertions(+), 4 deletions(-) diff --git a/README.md b/README.md index 6082ca5..51d2439 100644 --- a/README.md +++ b/README.md @@ -12,7 +12,7 @@ ## Introduction -**nf-core/readsimulator** is a pipeline to simulate sequencing reads. The pipeline currently supports simulating Amplicon, Target Capture, Metagenome, and Wholegenome data. It takes a samplesheet with sample names and seeds for random generation to produce simulated fastq files and a samplesheet that contains the paths to the fastq files. +**nf-core/readsimulator** is a pipeline to simulate sequencing reads. The pipeline currently supports simulating amplicon, target capture, metagenome, and wholegenome data. It takes a samplesheet with sample names and seeds for random generation to produce simulated FASTQ files and a samplesheet that contains the paths to the FASTQ files.