-
Notifications
You must be signed in to change notification settings - Fork 0
/
microrna.html
114 lines (103 loc) · 6.7 KB
/
microrna.html
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
<!DOCTYPE html>
<html lang="en">
<head>
<meta name="description" content="Lab website for Dr. Michael J. Betenbaugh, professor at The Johns Hopkins University. We specialize in glyco, cell, metabolic, and micro-algae engineering.">
<meta name="keywords" content="JHU, Johns Hopkins, Betenbaugh, professor, research, algae, biofuels, engineering, metabolic, cell, glyco, CHO">
<meta charset="utf-8">
<meta name="viewport" content="width=device-width, initial-scale=1">
<title>Betenbaugh Lab</title>
<!-- Bootstrap Core CSS -->
<link href="http://netdna.bootstrapcdn.com/bootstrap/3.0.3/css/bootstrap.min.css" rel="stylesheet" type="text/css">
<!-- Fonts -->
<link href="http://netdna.bootstrapcdn.com/font-awesome/4.0.3/css/font-awesome.min.css" rel="stylesheet" type="text/css">
<link href='http://fonts.googleapis.com/css?family=Lora:400,700,400italic,700italic' rel='stylesheet' type='text/css'>
<link href='http://fonts.googleapis.com/css?family=Montserrat:400,700' rel='stylesheet' type='text/css'>
<!-- Favicon -->
<link rel="icon" type="image/png" href="img/favicon.ico">
<!-- Custom Theme CSS -->
<link href="css/grayscale.css" rel="stylesheet">
</head>
<body id="page-top" data-spy="scroll" data-target=".navbar-custom">
<nav class="navbar navbar-custom navbar-fixed-top" role="navigation">
<div class="container">
<div class="navbar-header page-scroll">
<button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-main-collapse">
<i class="fa fa-bars"></i>
</button>
<a class="navbar-brand" href="index.html#page-top">
Betenbaugh Lab <span class="light"></span>
</a>
</div>
<!-- Collect the nav links, forms, and other content for toggling -->
<div class="collapse navbar-collapse navbar-right navbar-main-collapse">
<ul class="nav navbar-nav">
<!-- Hidden li included to remove active class from about link when scrolled up past about section -->
<li class="hidden">
<a href="#page-top"></a>
</li>
<li class="page-scroll">
<a href="index.html#about">About</a>
</li>
<li class="page-scroll">
<a href="index.html#research">Research</a>
</li>
<li class="dropdowns">
<a href="#" data-toggle="dropdown" class="dropdown-toggle">Members <b class="caret"></b></a>
<ul class="dropdown-menu">
<li><a href="professor.html">Professor Betenbaugh</a></li>
<li><a href="members.html">Members</a></li>
</ul>
</li>
<li class="dropdowns">
<a href="#" data-toggle="dropdown" class="dropdown-toggle">Tools and Links <b class="caret"></b></a>
<ul class="dropdown-menu">
<li><a href="GlycoFish">GlycoFish Database</a></li>
<li><a href="GlycoFly">GlycoFly Database</a></li>
<li><a href="publications.html">Publications</a></li>
<li><a href="tools.html">Other Tools</a></li>
</ul>
</li>
</ul>
</div>
<!-- /.navbar-collapse -->
</div>
<!-- /.container -->
</nav>
<br><br><br><br><br>
<div style="background-color:white; color:black">
<br><br><br>
<h1>micro-RNA Engineering</h1>
<div class="subtopics">
<a href="cell-engineering.html" class="btn btn-default btn-lg"><span class="glyphicon glyphicon-chevron-left"></span> Back</a>
<a href="apoptosis.html" class="btn btn-default btn-lg">Apoptosis Pathways</a>
<a href="membrane.html" class="btn btn-default btn-lg">Membrane Proteins</a>
<a href="delivery.html" class="btn btn-default btn-lg">Protein Delivery</a>
</div>
<br /><br /><br />
<p class="image"><img src="img/micro-rna-cho.JPG" /></p>
<br><br>
<p> microRNAs with their ability to regulate complex pathways that control cellular behavior and phenotype have been proposed as potential targets for cell engineering in the context of optimization of biopharmaceutical production cell lines, specifically of Chinese Hamster Ovary cells. However, until recently, research was limited by a lack of genomic sequence information on this industrially important cell line. With the publication of the genomic sequence and other relevant data sets for CHO cells since 2011, the doors have been opened for an improved understanding of CHO cell physiology and for the development of the necessary tools for novel engineering strategies. We have been researching the regulatory mechanisms of microRNAs obtained from other biological models and proof of concepts already performed on CHO cells, thus providing an outlook of potential applications of microRNA engineering in production cell lines.</p>
<br><br>
<h4>References</h4>
<p class="reference">Jadhav, Vaibhav, Matthias Hackl, Aliaksandr Druz, Smriti Shridhar, Cheng-Yu Chung, Kelley M. Heffner, David P. Kreil, et al. "CHO microRNA Engineering is Growing Up: Recent Successes and Future Challenges." Biotechnology Advances 31, no. 8 (12, 2013): 1501-1513.</p>
<p class="reference">Druz, Aliaksandr, Chia Chu, Brian Majors, Rodell Santuary, Michael Betenbaugh, and Joseph Shiloach. "A Novel microRNA Mmu-miR-466h Affects Apoptosis Regulation in Mammalian Cells." Biotechnology and Bioengineering 108, no. 7 (2011): 1651-1661.</p>
<br><br>
</div>
<p align="center"><span class="style1">© 2014 Johns Hopkins University</span></p>
<!-- Core JavaScript Files -->
<script src="https://ajax.googleapis.com/ajax/libs/jquery/1.10.2/jquery.min.js"></script>
<script src="http://netdna.bootstrapcdn.com/bootstrap/3.0.3/js/bootstrap.min.js"></script>
<script src="http://cdnjs.cloudflare.com/ajax/libs/jquery-easing/1.3/jquery.easing.min.js"></script>
<!-- Custom Theme JavaScript -->
<script src="js/grayscale.js"></script>
<!-- Google Analytics -->
<script>
(function(i,s,o,g,r,a,m){i['GoogleAnalyticsObject']=r;i[r]=i[r]||function(){
(i[r].q=i[r].q||[]).push(arguments)},i[r].l=1*new Date();a=s.createElement(o),
m=s.getElementsByTagName(o)[0];a.async=1;a.src=g;m.parentNode.insertBefore(a,m)
})(window,document,'script','//www.google-analytics.com/analytics.js','ga');
ga('create', 'UA-50123108-1', 'betenbaugh.org');
ga('send', 'pageview');
</script>
</body>
<!-- InstanceEnd --></html>