diff --git a/instrumentation/opentelemetry-instrumentation-jinja2/tests/test_jinja2.py b/instrumentation/opentelemetry-instrumentation-jinja2/tests/test_jinja2.py
index 542a65d220..87056f6e9d 100644
--- a/instrumentation/opentelemetry-instrumentation-jinja2/tests/test_jinja2.py
+++ b/instrumentation/opentelemetry-instrumentation-jinja2/tests/test_jinja2.py
@@ -143,7 +143,7 @@ def test_generate_inline_template(self):
     def test_file_template_with_root(self):
         with self.tracer.start_as_current_span("root"):
             loader = jinja2.loaders.FileSystemLoader(TMPL_DIR)
-            env = jinja2.Environment(loader=loader)
+            env = jinja2.Environment(loader=loader, autoescape=True)
             template = env.get_template("template.html")
             self.assertEqual(
                 template.render(name="Jinja"), "Message: Hello Jinja!"
@@ -164,7 +164,7 @@ def test_file_template_with_root(self):
 
     def test_file_template(self):
         loader = jinja2.loaders.FileSystemLoader(TMPL_DIR)
-        env = jinja2.Environment(loader=loader)
+        env = jinja2.Environment(loader=loader, autoescape=True)
         template = env.get_template("template.html")
         self.assertEqual(
             template.render(name="Jinja"), "Message: Hello Jinja!"