diff --git a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/__init__.py b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/__init__.py
index d37c45993c..0af25ef3bf 100644
--- a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/__init__.py
@@ -388,10 +388,11 @@ def _instrument(self, **kwargs):
 
         is_sql_commentor_enabled = kwargs.pop("is_sql_commentor_enabled", None)
 
+        opentelemetry_middleware_index = getattr(settings, 'OTEL_MIDDLEWARE_INDEX', 0)
         if is_sql_commentor_enabled:
-            settings_middleware.insert(0, self._sql_commenter_middleware)
+            settings_middleware.insert(opentelemetry_middleware_index, self._sql_commenter_middleware)
 
-        settings_middleware.insert(0, self._opentelemetry_middleware)
+        settings_middleware.insert(opentelemetry_middleware_index, self._opentelemetry_middleware)
 
         setattr(settings, _middleware_setting, settings_middleware)