diff --git a/OWLTools-Annotation/src/test/java/owltools/gaf/eco/EcoMapperTest.java b/OWLTools-Annotation/src/test/java/owltools/gaf/eco/EcoMapperTest.java index f7eed660f..dd32b64d7 100644 --- a/OWLTools-Annotation/src/test/java/owltools/gaf/eco/EcoMapperTest.java +++ b/OWLTools-Annotation/src/test/java/owltools/gaf/eco/EcoMapperTest.java @@ -24,37 +24,36 @@ public void basicTest() throws Exception { ParserWrapper p = new ParserWrapper(); OWLGraphWrapper graph = p.parseToOWLGraph(EcoMapper.ECO_PURL); EcoMapper mapper = EcoMapperFactory.createEcoMapper(graph); - + assertFalse(mapper.isGoEvidenceCode(null)); assertFalse(mapper.isGoEvidenceCode("FOO")); - + assertTrue(mapper.isGoEvidenceCode("IEA")); assertTrue(mapper.isGoEvidenceCode("ND")); assertTrue(mapper.isGoEvidenceCode("IPI")); - + assertEquals(graph.getOWLClassByIdentifier("ECO:0000353"), mapper.getEcoClassForCode("IPI")); - + assertEquals(2, mapper.getAllEcoClassesForCode("IGC").size()); } - + @Test public void checkMapping() throws Exception { final OntologyMapperPair pair = EcoMapperFactory.createTraversingEcoMapper(); TraversingEcoMapper mapper = pair.getMapper(); OWLGraphWrapper g = pair.getGraph(); - + single(mapper, g, "0000269", "EXP"); single(mapper, g, "0000318", "IBA"); single(mapper, g, "0000319", "IBD"); single(mapper, g, "0000305", "IC"); single(mapper, g, "0000314", "IDA"); - + single(mapper, g, "0000270", "IEP"); - + single(mapper, g, "0000316", "IGI"); single(mapper, g, "0000320", "IKR"); // see also IMR single(mapper, g, "0000315", "IMP"); - single(mapper, g, "0000320", "IMR"); // yes, same as IKR, IMR is considered deprecated single(mapper, g, "0000353", "IPI"); single(mapper, g, "0000321", "IRD"); single(mapper, g, "0000247", "ISA"); @@ -64,38 +63,36 @@ public void checkMapping() throws Exception { single(mapper, g, "0000307", "ND"); single(mapper, g, "0000245", "RCA"); single(mapper, g, "0000304", "TAS"); - - multiple(mapper, g, - Arrays.asList("0000317", "0000354"), - "IGC", + + multiple(mapper, g, + Arrays.asList("0000317", "0000354"), + "IGC", Arrays.asList("GO_REF:0000025")); - - multiple(mapper, g, - Arrays.asList("0000501", "0000256", "0000501", "0000501", - "0000265", "0000265", "0000501", "0000265", - "0000322", "0000323", "0000322", "0000323", - "0000265", "0000256", "0000363"), - "IEA", - Arrays.asList("GO_REF:0000002", "GO_REF:0000003", "GO_REF:0000004", "GO_REF:0000019", - "GO_REF:0000020", "GO_REF:0000023", "GO_REF:0000035", "GO_REF:0000037", - "GO_REF:0000038", "GO_REF:0000039", "GO_REF:0000040", "GO_REF:0000049", - "GO_REF:0000107", "GO_REF:0000108")); - - multiple(mapper, g, - Arrays.asList("0000250", "0000255", "0000031", "0000031"), - "ISS", + + multiple(mapper, g, + Arrays.asList("0007669", "0000256", "0007669", "0007669", + "0007669", "0000265", "0007669", "0007669", + "0007669", "0007669", "0000249", "0000363"), + "IEA", + Arrays.asList("GO_REF:0000002", "GO_REF:0000003", "GO_REF:0000004", "GO_REF:0000020", + "GO_REF:0000035", "GO_REF:0000041", "GO_REF:0000043", "GO_REF:0000116", + "GO_REF:0000044", "GO_REF:0000107", "GO_REF:0000108")); + + multiple(mapper, g, + Arrays.asList("0000250", "0000255", "0000031", "0000031"), + "ISS", Arrays.asList("GO_REF:0000011", "GO_REF:0000012", "GO_REF:0000027")); } - + private void single(TraversingEcoMapper mapper, OWLGraphWrapper ecoGraph, String eco, String go) { final OWLClass cls = mapper.getEcoClassForCode(go); assertEquals("http://purl.obolibrary.org/obo/ECO_"+eco, cls.getIRI().toString()); Set codes = mapper.getAllEcoClassesForCode(go); assertEquals(1, codes.size()); assertEquals(cls, codes.iterator().next()); - + checkEcoBranch(mapper, ecoGraph, cls); - + } private void checkEcoBranch(TraversingEcoMapper mapper, OWLGraphWrapper g, final OWLClass cls) { @@ -105,24 +102,24 @@ private void checkEcoBranch(TraversingEcoMapper mapper, OWLGraphWrapper g, final assertNotNull(e); OWLClass a = g.getOWLClassByIdentifier("ECO:0000217"); assertNotNull(a); - + Set ancestors = mapper.getAncestors(cls, false); String id = g.getIdentifier(cls); assertTrue("The "+id+" class should be a descendant of ECO:0000000 ! evidence", ancestors.contains(e)); assertFalse("The "+id+" class may not be a descendant of ECO:0000217 ! assertion method", ancestors.contains(a)); } - + private void multiple(TraversingEcoMapper mapper, OWLGraphWrapper ecoGraph, List ecos, String go, List refs) { final OWLClass cls = mapper.getEcoClassForCode(go); final String first = ecos.get(0); assertEquals("http://purl.obolibrary.org/obo/ECO_"+first, cls.getIRI().toString()); - + assertEquals(refs.size() + 1, ecos.size()); checkEcoBranch(mapper, ecoGraph, cls); - + Set all = new HashSet(); all.add(cls); - + for (int i = 0; i < refs.size(); i++) { String ref = refs.get(i); String currentEco = ecos.get(i+1); @@ -131,7 +128,7 @@ private void multiple(TraversingEcoMapper mapper, OWLGraphWrapper ecoGraph, List all.add(refCls); checkEcoBranch(mapper, ecoGraph, refCls); } - + Set codes = mapper.getAllEcoClassesForCode(go); assertEquals(all.size(), codes.size()); assertTrue(all.containsAll(codes)); diff --git a/OWLTools-Annotation/src/test/java/owltools/gaf/eco/TraversingEcoMapperTest.java b/OWLTools-Annotation/src/test/java/owltools/gaf/eco/TraversingEcoMapperTest.java index c876528d4..9a53b2082 100644 --- a/OWLTools-Annotation/src/test/java/owltools/gaf/eco/TraversingEcoMapperTest.java +++ b/OWLTools-Annotation/src/test/java/owltools/gaf/eco/TraversingEcoMapperTest.java @@ -33,20 +33,22 @@ public static void beforeClass() throws Exception { // where auxilery ontologies are not caught. The best wat to do that here is to load ECO // second and then do the merge. OWLOntology ont_main = pw.parse(getResourceIRIString("go_xp_predictor_test_subset.obo")); - OWLOntology ont_scnd = pw.parse(getResourceIRIString("eco.obo")); + //OWLOntology ont_scnd = pw.parse(getResourceIRIString("eco.obo")); + //OWLOntology ont_scnd = pw.parse(getResourceIRIString("eco-basic.20211012.obo")); + OWLOntology ont_scnd = pw.parse(getResourceIRIString("eco.20211012.obo")); g = new OWLGraphWrapper(ont_main); g.addSupportOntology(ont_scnd); - + // NOTE: This step is necessary or things will get ignored! // (This cropped-up in the loader at one point.) for (OWLOntology ont : g.getSupportOntologySet()) g.mergeOntology(ont); - + OWLReasonerFactory reasonerFactory = new ElkReasonerFactory(); r = reasonerFactory.createReasoner(g.getSourceOntology()); g.setReasoner(r); } - + @AfterClass public static void afterClass() throws Exception { if (g != null) { @@ -60,51 +62,71 @@ public static void afterClass() throws Exception { @Test public void testSimpleEco() throws Exception{ - /// - /// From: http://purl.obolibrary.org/obo/ECO_0000316 - /// IGI should be: "experimental evidence used in manual assertion" in: + // Create EcoTools instance. + TraversingEcoMapper eco = EcoMapperFactory.createTraversingEcoMapper(g, g.getReasoner(), true); + + /// + /// IGI + /// + /// From: http://purl.obolibrary.org/obo/ECO_0000316 + /// IGI should be: "experimental evidence used in manual assertion" in: /// + evidence - /// + experimental evidence - /// + experimental phenotypic evidence - /// + genetic interaction evidence - /// - genetic interaction evidence used in manual assertion - /// - - // Create EcoTools instance. - TraversingEcoMapper eco = EcoMapperFactory.createTraversingEcoMapper(g, g.getReasoner(), true); - - OWLClass igi = eco.getEcoClassForCode("IGI"); - assertNotNull("IGI must map to one OWLClass", igi); - - assertEquals("http://purl.obolibrary.org/obo/ECO_0000316", igi.getIRI().toString()); - String igiId = g.getIdentifier(igi); - assertEquals("ECO:0000316", igiId); - - String igiLabel = g.getLabel(igi); - assertEquals("genetic interaction evidence used in manual assertion", igiLabel); - - // Since we're reflexive, our six ancestors should be: - Set foo = new HashSet(); - foo.add("evidence"); // ECO:0000000 - foo.add("experimental evidence"); // ECO:0000006 - foo.add("experimental phenotypic evidence"); // ECO:0000059 - foo.add("genetic interaction evidence"); // ECO:0000011 - foo.add(igiLabel); // ECO:0000316 - + /// + experimental evidence + /// + experimental phenotypic evidence + /// + genetic interaction evidence + /// - genetic interaction evidence used in manual assertion + /// + + + OWLClass igi = eco.getEcoClassForCode("IGI"); + assertNotNull("IGI must map to one OWLClass", igi); + assertEquals("http://purl.obolibrary.org/obo/ECO_0000316", igi.getIRI().toString()); + String igiId = g.getIdentifier(igi); + assertEquals("ECO:0000316", igiId); + + String igiLabel = g.getLabel(igi); + assertEquals("genetic interaction evidence used in manual assertion", igiLabel); + + // Since we're reflexive, our six ancestors should be: + Set foo = new HashSet(); + foo.add("evidence"); + foo.add("experimental evidence"); + foo.add("experimental phenotypic evidence"); + foo.add("experimental phenotypic evidence used in manual assertion"); + foo.add("experimental evidence used in manual assertion"); + foo.add("genetic interaction evidence"); + foo.add(igiLabel); + // inferred by reasoner using cross products - foo.add("experimental evidence used in manual assertion"); // ECO:0000269 - - Set ecoSuperClasses = eco.getAncestors(igi, true); - - for( OWLClass ec : ecoSuperClasses ){ - String ec_str_label = g.getLabel(ec); - assertTrue("Actual ancestor should have been in hash, not: " + ec_str_label, - foo.contains(ec_str_label)); - } - - assertEquals(6, ecoSuperClasses.size()); + foo.add("evidence used in manual assertion"); + + Set ecoSuperClasses = eco.getAncestors(igi, true); + + for( OWLClass ec : ecoSuperClasses ){ + String ec_str_label = g.getLabel(ec); + assertTrue("Actual ancestor should have been in hash, not: " + ec_str_label, + foo.contains(ec_str_label)); + } + + assertEquals(8, ecoSuperClasses.size()); + + /// + /// IEA + /// + + OWLClass iea = eco.getEcoClassForCode("IEA"); + assertNotNull("IEA must map to one OWLClass", iea); + assertEquals("http://purl.obolibrary.org/obo/ECO_0007669", iea.getIRI().toString()); + String ieaId = g.getIdentifier(iea); + assertEquals("ECO:0007669", ieaId); + + Set ecoIeaSuperClasses = eco.getAncestors(iea, true); + + String ieaLabel = g.getLabel(iea); + assertEquals("computational evidence used in automatic assertion", ieaLabel); + + assertEquals(4, ecoIeaSuperClasses.size()); } - } diff --git a/OWLTools-Annotation/src/test/resources/eco-basic.20211012.obo b/OWLTools-Annotation/src/test/resources/eco-basic.20211012.obo new file mode 100644 index 000000000..564a9cd64 --- /dev/null +++ b/OWLTools-Annotation/src/test/resources/eco-basic.20211012.obo @@ -0,0 +1,19478 @@ +format-version: 1.2 +data-version: eco/releases/2021-10-08/eco-basic.owl +date: 08:10:2021 13:56 +subsetdef: go_groupings "Grouping classes used by GO" +subsetdef: valid_with_biological_process "GO biological process terms should be used in the GO with/from field" +subsetdef: valid_with_cellular_component "GO cellular component terms should be used in the GO with/from field" +subsetdef: valid_with_chemical_entity "Chemical entity IDs should be used in the GO with/from field" +subsetdef: valid_with_gene "Gene IDs should be used in the GO with/from field" +subsetdef: valid_with_molecular_function "GO molecular function terms should be used in the GO with/from field" +subsetdef: valid_with_protein "Protein IDs should be used in the GO with/from field" +subsetdef: valid_with_protein_complex "Protein complex IDs should be used in the GO with/from field" +subsetdef: valid_with_transcript "Transcript IDs should be used in the GO with/from field" +default-namespace: eco +remark: ECO (https://github.com/evidenceontology/evidenceontology) is released into the public domain under CC0 1.0 Universal (CC0 1.0). Anyone is free to copy, modify, or distribute the work, even for commercial purposes, without asking permission. Please see the Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/) for an easy-to-read description of CC0 1.0 or the full legal code (https://creativecommons.org/publicdomain/zero/1.0/legalcode) for more detailed information. To get a sense of why ECO is CC0 as opposed to licensed under CC-BY, please read this thoughtful discussion (https://github.com/OBOFoundry/OBOFoundry.github.io/issues/285) on the OBO Foundry GitHub site. +ontology: eco +property_value: http://purl.org/dc/elements/1.1/description "The Evidence & Conclusion Ontology (ECO) describes types of scientific evidence within the biological research domain that arise from laboratory experiments, computational methods, literature curation, or other means." xsd:string +property_value: http://purl.org/dc/elements/1.1/title "Evidence & Conclusion Ontology (ECO)" xsd:string +property_value: http://purl.org/dc/terms/license https://creativecommons.org/publicdomain/zero/1.0/ + +[Term] +id: ECO:0000000 +name: evidence +def: "A type of information that is used to support an assertion." [ECO:MCC] +synonym: "evidence code" RELATED [] +synonym: "evidence_code" RELATED [] +disjoint_from: ECO:0000217 ! assertion method + +[Term] +id: ECO:0000001 +name: inference from background scientific knowledge +def: "A type of curator inference where conclusions are drawn based on the background scientific knowledge of the curator." [ECO:SN] +is_a: ECO:0000205 ! curator inference + +[Term] +id: ECO:0000002 +name: direct assay evidence +alt_id: ECO:0005006 +def: "A type of experimental evidence resulting from the direct measurement of some aspect of a biological feature." [ECO:SN] +is_a: ECO:0000006 ! experimental evidence + +[Term] +id: ECO:0000003 +name: reconstitution assay evidence +def: "A type of direct assay evidence based on reconstructing a biological sample from its disassociated state to its original state." [ECO:KAV, PMID:26029343] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000004 +name: cell fractionation evidence +def: "A type of fractionation evidence where sub-cellular components are separated based on their physical properties such as density in a sucrose density gradient." [ECO:KIM, TAIR:TED] +comment: If using this term for Gene Ontology annotation, it would be used most typically for annotations to the cellular component ontology. +synonym: "cell fractionation" RELATED [] +is_a: ECO:0000100 ! fractionation evidence + +[Term] +id: ECO:0000005 +name: enzymatic activity assay evidence +alt_id: ECO:0005001 +def: "A type of protein assay evidence where the catalytic activity of an enzyme is determined." [url:http\://www.sciencedirect.com/science/article/pii/S2213020914000068] +synonym: "enzyme assay evidence" EXACT [] +synonym: "enzyme assays" RELATED [] +xref: MI:0415 "enzymatic study" +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000006 +name: experimental evidence +alt_id: ECO:0005023 +def: "A type of evidence resulting from manipulation of variables in order to discover cause and effect." [url:http\://holah.co.uk/page/experimental/] +subset: go_groupings +is_a: ECO:0000000 ! evidence + +[Term] +id: ECO:0000007 +name: immunofluorescence evidence +def: "A type of protein detection assay evidence where a fluorescently labeled antibody is used to detect the presence or localization of a biomolecule within a cell." [ECO:MCC, TAIR:TED] +synonym: "immunofluorescence" RELATED [] +is_a: ECO:0005517 ! protein detection assay evidence + +[Term] +id: ECO:0000008 +name: expression pattern evidence +def: "A type of experimental evidence that is based on characterization of gene expression." [ECO:MCC, GO:IEP] +comment: Use this evidence type when the annotation is inferred from the timing or location of expression of a gene. It may be difficult to determine whether the expression pattern truly indicates that a gene plays a role in a given process. +is_a: ECO:0000006 ! experimental evidence +property_value: IAO:0000112 "The 10 previously identified GlnR-regulated genes were all confirmed to be under GlnR control during nitrogen stress (i.e. differential expression in the wild type compared to the DeltaglnR mutant), but in addition a total of 392 genes were significantly up-regulated and 291 significantly down regulated (Additional file 1: Table S1). This indicates that GlnR mediates (directly or indirectly) the expression of over 680 genes." xsd:string {xref="PMID:23642041"} + +[Term] +id: ECO:0000009 +name: transcript expression evidence +alt_id: ECO:0000048 +def: "A type of expression pattern evidence where abundance of a transcript is analyzed." [ECO:RCT] +synonym: "transcript expression level evidence" EXACT [] +synonym: "transcriptomics evidence" EXACT [] +is_a: ECO:0000008 ! expression pattern evidence + +[Term] +id: ECO:0000010 +name: protein expression evidence +alt_id: ECO:0000046 +def: "A type of expression pattern evidence resulting from protein abundance quantification techniques." [NBK:22011, PMC:4029002, url:http\://www.informatics.jax.org/glossary/gain-of-function, url:https\://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-protein-expression-systems.html] +synonym: "protein expression level evidence" EXACT [] +is_a: ECO:0000008 ! expression pattern evidence +property_value: IAO:0000112 "In the BL21(DE3)(pJS429) cells, the levels of ClpP2s and VapC10 remained unchanged over a 2-hour period of translation arrest, but the VapB10 level showed to be decreased with a half-life (Figure 7C) similar to that observed in the strain BL21(DE3)(pJS883) (Figure 7B). These indicate that ClpXP2s could degrade VapB10 regardless of the presence or absence of VapC10." xsd:string {xref="PMID:24260461"} + +[Term] +id: ECO:0000011 +name: genetic interaction evidence +def: "A type of experimental phenotypic evidence resulting from the effect that a given gene has on another gene or genes, and the products." [ECO:RCT, PMID:11822023] +xref: TAIR:TED +is_a: ECO:0000059 ! experimental phenotypic evidence + +[Term] +id: ECO:0000012 +name: functional complementation evidence +def: "A type of genetic interaction evidence where a wild-type copy of the gene in question is inserted into a mutant cell to see if it restores the wild-type phenotype in the mutant background." [PMID:27403640] +synonym: "functional complementation" RELATED [] +is_a: ECO:0000011 ! genetic interaction evidence +property_value: IAO:0000112 "In addition, complementation of the ompR mutation in strain AR6 with plasmid pBR3 resulted in an increase in beta-galactosidase activity (1303 +- 80 Miller units), indicating that the His-tagged OmpR protein, expressed from the gene introduced in trans, was able to positively regulate flhDC expression." xsd:string {xref="PMID:20830609"} +property_value: IAO:0000112 "Trans-complementation of fimR on pDL276 (pHR6) in strain DeltafimR harboring pfim(445 b)-cat restored wild-type pfim expression (Figure S1). Taken together, pfim is negatively regulated by FimR." xsd:string {xref="PMID:23823757"} + +[Term] +id: ECO:0000013 +name: transgenic rescue experiment evidence +def: "A type of functional complementation evidence resulting from the introduction of a transgene to prevent, or \"rescue\" an organism from a condition." [url:http\://www.mdpi.com/1420-3049/19/9/13932/pdf, url:http\://www.nature.com/gt/journal/v11/n15/full/3302282a.html] +is_a: ECO:0000012 ! functional complementation evidence + +[Term] +id: ECO:0000015 +name: mutant phenotype evidence +def: "A type of experimental phenotypic evidence in which an observable phenotypic difference results from a change or mutation in DNA." [GO:IMP] +comment: Note that mutations need not be negative. Changes to DNA sequence (mutations) may be detrimental, have no impact, or be beneficial. +comment: The allele that encodes the phenotype most common in a particular natural population is referred to as the wild type allele, while any other form of that allele is known as the mutant form. +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000112 "Indeed, the GroEL protein appears to be more abundant in the LM3 wild type strain compared to the LM3-2 mutant strain, suggesting the involvement of the CcpA protein in the positive regulation of its expression." xsd:string {xref="PMID:17129387"} + +[Term] +id: ECO:0000016 +name: loss-of-function mutant phenotype evidence +def: "A type of mutant phenotype evidence where a phenotype is associated with altered gene product which lacks the molecular function of the wild-type gene." [SO:0002054] +is_a: ECO:0000015 ! mutant phenotype evidence + +[Term] +id: ECO:0000017 +name: ectopic expression evidence +def: "A type of experimental phenotypic evidence where a transgenic strain carrying the construct of a promoter cDNA fusion in which a gene of interest is driven by a defined promoter or enhancer is ectopically expressed in the defined pattern to characterize potential cellular properties and functions of a protein of interest." [PMID:10948520, PMID:19301619] +synonym: "analysis of overexpression/ectopic expression phenotype" RELATED [] +is_a: ECO:0000059 ! experimental phenotypic evidence + +[Term] +id: ECO:0000018 +name: anti-sense experiment evidence +def: "A type of mutant phenotype evidence where a phenotype is observed while expressing an anti-sense version of a gene product in a wild-type (for that gene product) background." [ECO:SN] +synonym: "anti-sense experiments" RELATED [] +is_a: ECO:0000015 ! mutant phenotype evidence + +[Term] +id: ECO:0000019 +name: RNAi evidence +def: "A type of mutant phenotype evidence where an RNA construct is introduced into a cell and the expression of the gene bearing its complementary sequence is suppressed." [ECO:MCC] +synonym: "RNAi experiment" RELATED [] +is_a: ECO:0000015 ! mutant phenotype evidence + +[Term] +id: ECO:0000020 +name: protein inhibition evidence +def: "A type of direct assay evidence based on the inhibition of the molecular function of a protein." [ECO:MCC] +synonym: "specific protein inhibition evidence" NARROW [] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000021 +name: physical interaction evidence +alt_id: ECO:0005025 +def: "A type of experimental evidence that is based on characterization of an interaction between a gene product and another molecule." [ECO:SN] +comment: Molecules interacted with might include protein, nucleic acid, ion, or complex. +xref: MI:0045 "experimental interaction detection" +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000022 +name: co-purification evidence +def: "A type of physical interaction evidence where a cellular component subunit is isolated as part of purification of its larger complex." [TAIR:TED] +synonym: "co-purification" RELATED [] +xref: MI:0025 "copurification" +is_a: ECO:0000021 ! physical interaction evidence + +[Term] +id: ECO:0000023 +name: affinity evidence +def: "A type of physical interaction evidence that depends on the strength of the interaction between two entities." [ECO:MCC, PSI-MI:MI\:0400] +synonym: "ligand binding evidence" NARROW [] +xref: MI:0400 "affinity technology" +is_a: ECO:0000021 ! physical interaction evidence + +[Term] +id: ECO:0000024 +name: protein binding evidence +def: "A type of affinity evidence resulting from the binding of a molecule to a protein or protein complex." [GO:0005515, url:https\://en.wikipedia.org/wiki/Mutation] +is_a: ECO:0000023 ! affinity evidence + +[Term] +id: ECO:0000025 +name: bait-prey hybrid interaction evidence +def: "A type of protein binding evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090] +comment: Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode. +synonym: "bait-prey protein pull-down evidence" EXACT [] +xref: MI:0090 "protein complementation assay" +is_a: ECO:0000024 ! protein binding evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string + +[Term] +id: ECO:0000026 +name: nucleic acid hybridization evidence +def: "A type of experimental genomic evidence resulting from a process in which single stranded nucleic acids are allowed to interact so that complexes, or hybrids, are formed by molecules with sufficiently similar, complementary sequences." [OBI:0302903] +comment: This term has been made obsolete with the clean up of experimental genomic evidence branch. +is_obsolete: true + +[Term] +id: ECO:0000027 +name: structural similarity evidence +def: "A type of similarity evidence based on structural similarity of an annotated gene or gene product to another gene or group of genes." [ECO:MCC, TAIR:TED] +comment: For GO annotation, in the case of a single gene, an accession for the related gene's sequence is entered in the evidence_with field. +is_a: ECO:0000057 ! phenotypic similarity evidence +property_value: IAO:0000112 "The secondary structure of VapC10 (Figure S2), predicted with the 3DJIGSAW prediction tool [36] and the DALI server [37], exhibited homology with several well studied VapC toxins, such as the first PIN domain structure for the protein PAE2754 from the archae bacterium Pyrobaculum aerophilum (30)." xsd:string {xref="PMID:24260461"} + +[Term] +id: ECO:0000028 +name: motif similarity evidence +def: "A type of match to sequence model evidence that is based on the presence of a recognized domain or motif in a gene product's (usually protein) primary sequence." [ECO:SN] +synonym: "recognized domains" RELATED [] +is_a: ECO:0000202 ! match to sequence model evidence +property_value: IAO:0000112 "A CRP box-like sequence was found in the promoter-proximate region of sycO-ypkA-yopJ [4], indicating the direct association of CRP with the sycO-ypkA-yopJ promoter region." xsd:string {xref="PMID:19703315"} +property_value: IAO:0000112 "To identify further CopR target genes, the binding motif TGAAGATTTnnTGAAGATTT was used to search for similar sequences in the whole C. glutamicum genome using the ERGO (TM) bioinformatics suite (Integrated Genomics, Illinois, USA) allowing four mutations, no deletions and no insertions. 46 hits were found, but only in six cases the putative CopR binding site was located in intergenic regions up to 200 bp upstream of the start codon of the neighbouring gene (cg1336, cg2976, cg3187, cg3337, cg3357 and cg0414)." xsd:string {xref="PMID:21799779"} + +[Term] +id: ECO:0000029 +name: match to InterPro member signature evidence +def: "A type of match to sequence model evidence resulting from a positive match of a protein, or set of proteins to a predictive model (signature) in the InterPro database." [PMC:2686546, url:http\://www.ncbi.nlm.nih.gov/mesh?term=Nucleic+Acid+Hybridization] +is_a: ECO:0000202 ! match to sequence model evidence + +[Term] +id: ECO:0000030 +name: BLAST evidence used in manual assertion +def: "A type of BLAST evidence that is used in a manual assertion." [ECO:MCC] +synonym: "curated BLAST analysis" RELATED [] +is_a: ECO:0000206 ! BLAST evidence +is_a: ECO:0005560 ! pairwise sequence alignment evidence used in manual assertion +property_value: ECO:9000002 "ISA" xsd:string + +[Term] +id: ECO:0000031 +name: protein BLAST evidence used in manual assertion +def: "A type of protein BLAST evidence that is used in a manual assertion." [ECO:SN] +synonym: "curated protein BLAST analysis" RELATED [] +xref: GO_REF:0000012 "Pairwise alignment (TIGR)" +xref: GO_REF:0000027 "BLAST search criteria for ISS assignment in PAMGO_GAT" +is_a: ECO:0000030 ! BLAST evidence used in manual assertion +is_a: ECO:0000208 ! protein BLAST evidence +property_value: ECO:9000002 "ISA" xsd:string + +[Term] +id: ECO:0000032 +name: nucleotide BLAST evidence used in manual assertion +def: "A type of nucleotide BLAST evidence that is used in a manual assertion." [ECO:SN] +synonym: "curated nucleic acid BLAST analysis" RELATED [] +is_a: ECO:0000030 ! BLAST evidence used in manual assertion +is_a: ECO:0000207 ! nucleotide BLAST evidence +property_value: ECO:9000002 "ISA" xsd:string + +[Term] +id: ECO:0000033 +name: author statement supported by traceable reference +def: "A type of author statement in which the author makes a statement that is not supported by information in that particular publication, but rather can be traced to a reference cited by that publication." [ECO:RCT, GO:TAS] +synonym: "traceable author statement" RELATED [] +is_a: ECO:0000204 ! author statement +disjoint_from: ECO:0000034 ! author statement without traceable support + +[Term] +id: ECO:0000034 +name: author statement without traceable support +def: "A type of author statement that is not associated with results presented or a cited reference." [ECO:SN] +synonym: "non-traceable author statement" RELATED [] +is_a: ECO:0000204 ! author statement + +[Term] +id: ECO:0000035 +name: no evidence data found +def: "A type of curator inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources." [ECO:SN] +comment: An assertion of "no evidence data found" carries the assumption that a more-or-less exhaustive search has been conducted. +is_a: ECO:0000205 ! curator inference + +[Term] +id: ECO:0000037 +name: not_recorded +comment: The evidence not_recorded appears in some legacy annotations; it should not be used for new annotations. +is_obsolete: true + +[Term] +id: ECO:0000038 +name: transient rescue experiment evidence +def: "A type of functional complementation evidence resulting from the introduction of nucleic acids, which are not permanently incorporated into the genome, to temporarily prevent, or \"rescue\" an organism from a condition." [] +is_a: ECO:0000012 ! functional complementation evidence + +[Term] +id: ECO:0000039 +name: protein assay evidence +alt_id: ECO:0005022 +def: "A type of direct assay evidence resulting from determining the presence, abundance, structure, function, or activity of proteins." [PMID:18429326] +is_obsolete: true + +[Term] +id: ECO:0000040 +name: immunological assay evidence +alt_id: ECO:0005018 +def: "A type of affinity evidence resulting from quantitation of the analyte which depends on the reaction of an antigen (analyte) and an antibody." [ERO:0001362, url:http\://www.ncbi.nlm.nih.gov/books/NBK21589/\,http\://cores.ucsf.edu/protein-assay.html] +is_a: ECO:0000023 ! affinity evidence + +[Term] +id: ECO:0000041 +name: similarity evidence +def: "A type of evidence resulting from comparing likeness of distinct biological entities." [ECO:SN] +synonym: "inferred from similarity" RELATED [] +synonym: "IS" EXACT [] +is_a: ECO:0000000 ! evidence + +[Term] +id: ECO:0000042 +name: gain-of-function mutant phenotypic evidence +def: "A type of mutant phenotype evidence resulting from an altered gene product which possesses a new molecular function or a new pattern of gene expression." [SO:0002053, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614608/] +synonym: "gain-of-function mutant phenotype evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence + +[Term] +id: ECO:0000044 +name: sequence similarity evidence +def: "A type of similarity based on biomolecular sequence." [ECO:MCC, TAIR:TED] +comment: A sequence similarity analysis may involve a gene or a gene product, and it could be based on similarity to a single other gene or to a group of other genes. +is_a: ECO:0000041 ! similarity evidence +is_a: ECO:0007672 ! computational evidence + +[Term] +id: ECO:0000045 +name: spatial pattern of protein expression evidence +def: "A type of protein expression evidence that accounts for the position of RNA translation to a protein product ranging in scale from differing tissues to regions of an anatomical structure." [] +is_a: ECO:0000010 ! protein expression evidence + +[Term] +id: ECO:0000047 +name: spatial pattern of transcript expression evidence +def: "A type of transcript expression evidence that accounts for the position of transcription ranging in scale from the position of genes on the chromosome to differing tissues to regions of an anatomical structure." [] +is_a: ECO:0000009 ! transcript expression evidence + +[Term] +id: ECO:0000049 +name: reporter gene assay evidence +def: "A type of expression pattern evidence that is based on the expression pattern of a reporter gene." [TAIR:TED] +synonym: "expression of a reporter gene" RELATED [] +is_a: ECO:0000008 ! expression pattern evidence + +[Term] +id: ECO:0000050 +name: voucher specimen phenotypic analysis evidence +def: "A type of experimental phenotypic evidence based on an authors phenotypic description of a species (or higher-level group), which explicitly references an observation made of a voucher specimen (a specimen with a permanent museum catalog)." [TAIR:TED] +synonym: "IVS" EXACT [] +synonym: "voucher specimen analysis evidence" EXACT [] +is_a: ECO:0000059 ! experimental phenotypic evidence + +[Term] +id: ECO:0000051 +name: genetic similarity evidence +def: "A type of similarity based on genotype without respect to expression." [ECO:MCC, PhenoScape:IGTS] +comment: A genetic similarity analysis might consider genetic markers, polymorphisms, alleles, or other characteristics sometimes considered as part of the field of traditional genetics. Although an attempt has been made to treat as distinct the concepts of "genetic", "genotypic", "genomic", and "sequence", there is considerable overlap in usage throughout the field of biology. +synonym: "IGTS" EXACT [] +synonym: "inferred from genetic similarity" RELATED [] +is_a: ECO:0000041 ! similarity evidence + +[Term] +id: ECO:0000052 +name: suppressor/enhancer interaction phenotypic evidence +def: "A type of genetic interaction evidence resulting from a second mutation that either suppresses or enhances the phenotype expressed from an original mutation." [url:http\://biorxiv.org/content/early/2015/10/03/021592, url:http\://www.wormbook.org/chapters/www\:geneticsuppression/geneticsuppression.html] +synonym: "'traditional' genetic interactions (e.g. suppressors, synthetic lethals)" RELATED [] +synonym: "suppressor/enhancer interaction evidence" EXACT [] +xref: TAIR:TED +is_a: ECO:0000011 ! genetic interaction evidence + +[Term] +id: ECO:0000053 +name: automatically integrated combinatorial evidence used in automatic assertion +alt_id: ECO:0000246 +def: "A type of automatically integrated combinatorial evidence that is used in an automatic assertion." [ECO:MCC, ECO:RCT] +comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments. +xref: TAIR:TED +is_a: ECO:0000213 ! combinatorial evidence used in automatic assertion +is_a: ECO:0007673 ! automatically integrated combinatorial evidence +property_value: ECO:9000002 "IEA" xsd:string + +[Term] +id: ECO:0000054 +name: double mutant phenotypic evidence +def: "A type of mutant phenotype evidence resulting from an experiment typically constructed to determine if two different genes have an observable genetic interaction (functional connection) as the result of a mutation occurring in the alleles of the two genes of interest." [ECO:RCT, PMID:18305163] +synonym: "double mutant analysis" RELATED [] +synonym: "double mutant phenotype evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000112 "In the hns slyA double mutant, hlyE expression was twofold greater than in the parent, and slightly higher than the hns single mutant, suggesting that slyA has a small negative effect on hlyE expressionin the absence of H-NS." xsd:string {xref="PMID:17892462"} + +[Term] +id: ECO:0000055 +name: array experiment evidence +comment: This general 'array experiment' term should not be used - replace with the specific type of array (e.g. ECO:0000097, ECO:0000062, ECO:0000104, etc.) +is_obsolete: true + +[Term] +id: ECO:0000056 +name: epistatic interaction phenotypic evidence +def: "A type of genetic interaction evidence resulting from the suppression of one allelic effect by an allele at another genetic locus." [PMID:18852697] +comment: Epistasis' can be used in different contexts in different areas of genetics. It is sometimes used to mean 'genetic interaction', whereas other times it may be specific to mutations that block the effects of other mutations. +synonym: "epistatic interaction evidence" EXACT [] +synonym: "epistatic interactions" RELATED [] +is_a: ECO:0000011 ! genetic interaction evidence + +[Term] +id: ECO:0000057 +name: phenotypic similarity evidence +def: "A type of similarity based on the expression of a genotype in an environment." [ECO:MCC, PhenoScape:IPTS] +comment: Phenotype is defined as the outcome of the expression of a genotype in a given environment. A comparison might involve whole organisms or sub-parts of organisms. +synonym: "inferred from phenotypic similarity" RELATED [] +synonym: "IPTS" EXACT [] +synonym: "phenotype similarity evidence" EXACT [] +is_a: ECO:0000041 ! similarity evidence + +[Term] +id: ECO:0000058 +name: expression microarray evidence +alt_id: ECO:0000356 +def: "A type of transcript expression evidence resulting from simultaneous profiling of the expression levels of thousands of genes in a single experiment allowing analysis of genes and their networks." [url:http\://www.illumina.com/techniques/microarrays/gene-expression-arrays.html, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435252/] +synonym: "differential gene expression evidence from microarray experiment" NARROW [] +is_a: ECO:0000009 ! transcript expression evidence +property_value: IAO:0000112 "S. lividans AdpA directly regulates at least the six AdpA-dependent genes listed above and identified by microarrays and qRT-PCR analysis." xsd:string {xref="PMID:24694298"} +property_value: IAO:0000112 "To identify the regulon of the response regulator CopR, the transcriptome of the DeltacopRS deletion mutant was compared to that of the wild type using DNA microarrays. For cells grown in standard CGXII medium (1.25 microM CuSO4), no significant gene expression differences were observed, indicating that the CopRS two-component system is not active under this condition (data not shown)." xsd:string {xref="PMID:21799779"} + +[Term] +id: ECO:0000059 +name: experimental phenotypic evidence +alt_id: ECO:0000014 +alt_id: ECO:0005017 +def: "A type of experimental evidence that is based on an observable characteristic trait, which is the result of the expression of an organisms genotype in an environment." [ECO:SN] +comment: Observable characteristic traits can be morphology, development, behavior, biochemical or physiological properties, etc. +synonym: "inferred from phenotype" RELATED [] +is_a: ECO:0000006 ! experimental evidence + +[Term] +id: ECO:0000060 +name: positional similarity evidence +def: "A type of phenotypic similarity evidence based on the similarity of stucture locations or arrangements." [TAIR:TED] +synonym: "IPS" EXACT [] +is_a: ECO:0000057 ! phenotypic similarity evidence + +[Term] +id: ECO:0000061 +name: quantitative trait analysis evidence +def: "A type of phenotypic evidence that is based on a gene product that is associated with a quantitative trait locus, but has not been cloned." [TAIR:TED] +synonym: "QTL analysis evidence" EXACT [] +synonym: "quantitative trait analysis" RELATED [] +is_a: ECO:0000059 ! experimental phenotypic evidence + +[Term] +id: ECO:0000062 +name: cRNA to DNA expression microarray evidence +def: "A type of expression microarray evidence where expression level is quantified by sample biotin-labeled cRNA (transcribed from an unknown RNA sample) hybridized to DNA oligonuclotides immoblized on a solid surface." [url:https\://link.springer.com/chapter/10.1007/978-94-017-9716-0_30] +synonym: "genomic microarray evidence" EXACT [] +is_a: ECO:0000058 ! expression microarray evidence + +[Term] +id: ECO:0000063 +name: compositional similarity evidence +def: "A type of phenotypic similarity evidence based on the similarity of the histological makeup of structures." [TAIR:TED] +synonym: "ICS" EXACT [] +is_a: ECO:0000057 ! phenotypic similarity evidence + +[Term] +id: ECO:0000064 +name: functional complementation in heterologous system evidence +def: "A type of functional complementation evidence that is based on the insertion of a wild-type copy of a gene into a heterologous organism, with the mutation occurring in a homologous gene." [TAIR:TED] +synonym: "functional complementation in heterologous system" RELATED [] +is_a: ECO:0000012 ! functional complementation evidence + +[Term] +id: ECO:0000066 +name: yeast one-hybrid evidence +def: "A type of bait-prey hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors." [ECO:MCC, PSI-MI:MI\:0432, TAIR:TED] +comment: The assay involves screening a library of candidate proteins for the ability bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to a yeast reporter gene (TAIR:TED). +synonym: "yeast one-hybrid assay" RELATED [] +xref: MI:0432 "one hybrid" +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence + +[Term] +id: ECO:0000067 +name: developmental similarity evidence +def: "A type of phenotypic similarity evidence based on the similarity of embryological or post-embryonic orgin of structures." [TAIR:TED] +synonym: "IDS" EXACT [] +is_a: ECO:0000057 ! phenotypic similarity evidence + +[Term] +id: ECO:0000068 +name: yeast 2-hybrid evidence +def: "A type of bait-prey hybrid interaction evidence that is based on detection of protein-protein interaction by activation of a yeast reporter gene after a bait protein fused to a DNA-binding domain (which has been transfected into a yeast cell) is used to screen a cDNA library of clones fused to an activation domain." [PMID:12734586] +synonym: "yeast two-hybrid assay" RELATED [] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence + +[Term] +id: ECO:0000069 +name: differential methylation hybridization evidence +alt_id: ECO:0000065 +def: "A type of in vitro methylation assay evidence where methylation-sensitive restriction enzymes are utilized to compare differential methylation between two samples by first shearing DNA, followed by end-blunting, ligation of linkers, methylation sensitive restriction, PCR using linker primers, dye labeling and relative quantification of methylated DNA fragments by two-colored array hybridization to a CpG island microarray for visual assessment." [PMID:18987809] +comment: Color is indicative of methylation status - be it hypo- (pseudo-green), hyper- (pseudo-red), or equal methylation (pseudo-yellow). +synonym: "CpG island microarray evidence" EXACT [] +is_a: ECO:0001065 ! in vitro methylation assay evidence + +[Term] +id: ECO:0000070 +name: co-immunoprecipitation evidence +def: "A type of immunoprecipitation evidence that involves precipitating two or more proteins via binding to an antibody specific to a single protein, followed by protein identification." [ECO:MCC] +comment: If performing GO annotation, the interacting protein is referenced in the evidence_with column. +synonym: "co-immunoprecipitation" RELATED [] +xref: MI:0019 "coimmunoprecipitation" +is_a: ECO:0000085 ! immunoprecipitation evidence + +[Term] +id: ECO:0000071 +name: morphological similarity evidence +def: "A type of phenotypic similarity evidence based on the similarity of shape, structure, or configuration in structures." [TAIR:TED] +synonym: "IMS" EXACT [] +is_a: ECO:0000057 ! phenotypic similarity evidence + +[Term] +id: ECO:0000072 +name: Sos-recruitment assay evidence +is_obsolete: true + +[Term] +id: ECO:0000073 +name: experimental genomic evidence +def: "A type of experimental evidence that is based on the characterization of an attribute of the genome underlying a gene product." [ECO:MCC] +comment: This term has been made obsolete with the clean up of experimental genomic evidence branch. +synonym: "inferred from genomic analysis" EXACT [] +is_obsolete: true + +[Term] +id: ECO:0000074 +name: split-ubiquitin functional complementation evidence +def: "A type of protein fragment functional complementation evidence that is based on detection of protein-protein interaction between a bait and prey protein by in vivo reconstitution of split-ubiquitin (when bait and prey interact) and release of a reporter protein." [PMID:15064465] +comment: The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquitin (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough to reconstitute.TAIR:TED +synonym: "split-ubiquitin assay" RELATED [] +xref: MI:0112 "ubiquitin reconstruction" +is_a: ECO:0006256 ! protein fragment functional complementation evidence + +[Term] +id: ECO:0000075 +name: gene expression similarity evidence +def: "A type of phenotypic similarity evidence that is based on the categorization of genes by the similarity of expression profiles." [PMID:19958477] +synonym: "IGES" EXACT [] +is_a: ECO:0000057 ! phenotypic similarity evidence + +[Term] +id: ECO:0000076 +name: far-Western blotting evidence +def: "A type of physical interaction evidence that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes." [PMID:18079728] +comment: The interacting protein is referenced in the evidence_with column. +synonym: "far-Western analysis" RELATED [] +xref: MI:0047 +is_a: ECO:0000021 ! physical interaction evidence + +[Term] +id: ECO:0000077 +name: methylation-specific polymerase chain reaction evidence +def: "A type of in vitro methylation assay evidence resulting from initial modification of DNA by sodium bisulfite, converting all unmethylated cytosines to uracil, and subsequent amplification with primers specific for methylated versus unmethylated DNA." [PMC:38513, PMID:8790415] +comment: The product is the amplification of existing sequences - i.e. more DNA that can be further processed e.g. sequenced, DNA fingerprinting. +synonym: "methylation-specific PCR evidence" EXACT [] +synonym: "MS-PCR" EXACT [] +synonym: "MSP" EXACT [] +is_a: ECO:0001065 ! in vitro methylation assay evidence + +[Term] +id: ECO:0000078 +name: southern hybridization evidence +def: "A type of DNA detection assay evidence that is based on detection of a specific DNA sequence by hybridization of labeled probes to any immobilized DNA fragment with sequence similarity." [PMID:18432697] +comment: The DNA fragments are prepared through gel electrophoresis then transferred to a filter membrane. +synonym: "Southern blot" EXACT [] +synonym: "Southern blotting" RELATED [] +is_a: ECO:0005519 ! DNA detection assay evidence + +[Term] +id: ECO:0000079 +name: affinity chromatography evidence +def: "A type of affinity evidence that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bound compound." [ECO:MCC, TAIR:TED] +comment: "Used when an annotation is made based on affinity chromatography, which is a selective separation technique by which a compound (e.g., an antibody) is immobilized on a polymeric matrix and used to bind selectively other compounds. Following removal of the unattached components, the bound compound is displaced by changing the concentration of protons, salts, or cofactors in the eluent" (from original definition by TAIR:TED). Types of highly specific interaction might include those of antigen and antibody, enzyme and substrate, or receptor and ligand. +synonym: "affinity chromatography" RELATED [] +xref: MI:0004 "affinity chromatography technology" +is_a: ECO:0000023 ! affinity evidence + +[Term] +id: ECO:0000080 +name: phylogenetic evidence +def: "A type of similarity that indicates common ancestry." [ECO:MCC, PhenoScape:IP] +synonym: "IP" EXACT [PhenoScape:IP] +is_a: ECO:0000041 ! similarity evidence +property_value: IAO:0000112 "Comparative phylogenomics along with MLST (multilocus sequence typing) and whole genome sequecing has shown that ribotype 078 lineage is different than other C. difficile lineages [22]." xsd:string {xref="PMID:24713082"} + +[Term] +id: ECO:0000081 +name: targeting sequence prediction evidence +def: "A type of motif similarity evidence that is based on detection of a targeting sequence in the primary sequence of a protein through computational prediction and/or a manual examination of the sequence." [ECO:RCT, TAIR:TED] +synonym: "targeting sequence prediction" RELATED [] +is_a: ECO:0000028 ! motif similarity evidence + +[Term] +id: ECO:0000082 +name: polymerase chain reaction evidence +def: "A type of experimental genomic evidence where DNA polymerase is used to synthesize new strand of DNA complementary to the offered template strand." [OBI:0000415] +comment: This term has been made obsolete because PCR is not evidence. The children have been appropriately placed under other parents. +synonym: "PCR evidence" EXACT [] +is_obsolete: true + +[Term] +id: ECO:0000083 +name: transmembrane domain prediction evidence +def: "A type of motif similarity evidence that is based on detection of one, or more, transmembrane domains in the primary sequence of a protein through computational prediction and/or a manual examination of the sequence." [ECO:RCT, TAIR:TED] +synonym: "transmembrane domain prediction" RELATED [] +is_a: ECO:0000028 ! motif similarity evidence + +[Term] +id: ECO:0000084 +name: gene neighbors evidence +def: "A type of genomic context evidence in which a gene product's identity is supported on the basis of the identity of neighboring genes." [ECO:MCC, GOC:MG] +comment: Genomic cluster analyses include synteny and operon structure. +synonym: "ICL" RELATED [] +synonym: "inferred from genome cluster" EXACT [] +xref: GO_REF:0000025 "Operon structure as IGC evidence" +is_a: ECO:0000177 ! genomic context evidence +created_by: mgiglio +creation_date: 2009-03-20T11:55:18Z + +[Term] +id: ECO:0000085 +name: immunoprecipitation evidence +def: "A type of protein binding evidence that involves precipitation of a multivalent antigen by a bivalent antibody for protein isolation." [ECO:MCC, ECO:SW, TAIR:TED] +comment: Transcription factors isolated in this way can be incubated with a radiolabeled probe to demonstrate binding. +synonym: "immunoprecipitation" RELATED [] +is_a: ECO:0000024 ! protein binding evidence +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000086 +name: amplification of intermethylated sites evidence +def: "A type of in vitro methylation assay evidence resulting from a genome-wide estimate of DNA methylation by differential enzymatic digestion of genomic DNA with methylation-sensitive and methylation-insensitive isoschizomers, followed by restrained PCR amplification of sequences methylated at both ends and resolving the PCR products in a denaturing polyacrylamide-sequencing gel to generate fingerprints that consist of multiple anonymous bands that represent the DNA methylome of the cell." [PMID:18987810, url:https\://www.ncbi.nlm.nih.gov/probe/docs/techpcr/] +comment: The bands can be individually isolated and characterized which leads to the identification of hypo- and hypermethylation events. +synonym: "AIMS" EXACT [] +is_a: ECO:0001065 ! in vitro methylation assay evidence + +[Term] +id: ECO:0000087 +name: immunolocalization evidence +def: "A type of microscopy evidence where antibodies are used to detect the location of molecules or other structures within cells or tissues." [ECO:MCC, ECO:SN] +synonym: "immunolocalization" RELATED [] +is_a: ECO:0001098 ! microscopy evidence +is_a: ECO:0005593 ! immunodetection assay evidence + +[Term] +id: ECO:0000088 +name: biological system reconstruction evidence +def: "A type of evidence that is based on a combination of experimental evidences or existing models of that system in a related species used for the reconstruction of a biological system." [ECO:MCC, GOC:mg] +comment: The biological system in question might be a multi-step process or pathway or a physical complex comprising several components. The components in the experimental evidence can come from the same species or a mix of species. The experimental evidences may be only partial or weak. +synonym: "inferred from system reconstruction" RELATED [] +synonym: "ISR" RELATED [] +is_a: ECO:0000000 ! evidence +created_by: mgiglio +creation_date: 2009-03-20T12:00:17Z + +[Term] +id: ECO:0000089 +name: restriction landmark genomic scanning evidence +alt_id: ECO:0001125 +def: "A type of DNA detection assay evidence resulting from the use of restriction enzymes for direct end-labeling of DNA (creating landmarks), followed by high-resolution two-dimensional electrophoresis to visualize the landmarks." [PMID:8388788] +synonym: "restriction landmark genome scanning evidence" EXACT [] +synonym: "RLGS evidence" EXACT [] +is_a: ECO:0005519 ! DNA detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string + +[Term] +id: ECO:0000090 +name: immunogold labelling evidence +def: "A type of immunolocalization evidence where the antibodies are labeled with colloidal gold particles whose location is then detected via microscopy." [ECO:MCC] +synonym: "immunogold labelling" RELATED [] +is_a: ECO:0000087 ! immunolocalization evidence + +[Term] +id: ECO:0000092 +name: epitope-tagged protein immunolocalization evidence +def: "A type of immunolocalization evidence in which recombinant proteins fused with epitopes are recognized by antibodies." [TAIR:TED] +synonym: "immunolocalization of epitope-tagged protein" RELATED [] +is_a: ECO:0000087 ! immunolocalization evidence + +[Term] +id: ECO:0000093 +name: array-based sequence capture evidence +def: "A type of DNA detection assay evidence evidence where gene sequence or variation is determined or detected by fluorescently-labeled DNA fragments hybridized to sequence-specific oligonucleotides immobilized on an array." [PMID:21049075] +synonym: "DNA microarray" BROAD [] +synonym: "oligonucleotide microarray evidence" BROAD [] +synonym: "single nucleotide polymorphism array evidence" NARROW [] +synonym: "SNP array evidence" NARROW [] +is_a: ECO:0005519 ! DNA detection assay evidence + +[Term] +id: ECO:0000094 +name: biological assay evidence +comment: The children of 'biological assay evidence' have been moved under 'direct assay evidence', and this term has been deprecated with no children left. +is_obsolete: true + +[Term] +id: ECO:0000095 +name: cell growth regulation assay evidence +def: "A type of cell growth assay evidence that measures one or more aspects of cell growth over a specified time period to indicate an induced or repressed regulatory effect." [ECO:SW, PomBase:MAH] +comment: Cell growth aspects can include growth rate and extent of growth. A cell growth curve acts as a natural reporter. +synonym: "growth curve analysis" RELATED [] +is_a: ECO:0001563 ! cell growth assay evidence +created_by: mchibucos +creation_date: 2014-10-15T00:58:50Z + +[Term] +id: ECO:0000096 +name: electrophoretic mobility shift assay evidence +def: "A type of affinity evidence based on an electrophoretic mobility shift of macromolecules, where proteins, nucleic acids, or both, are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect interactions/complexes between proteins and/or nucleic acid." [ECO:KIM, ECO:SW, PMID:17703195, TAIR:TED] +comment: EMSA is often used for assessing TF-binding with fluorophore labelling. Protein-nucleic acid complexes generally migrate at a slower rate than the corresponding non-bonded nucleic acid. +synonym: "electrophoretic mobility shift assay" RELATED [] +synonym: "EMSA: electrophoretic mobility shift assay" EXACT [] +synonym: "Gel retardation assay" RELATED [PMID:17703195] +xref: MI:0413 "electrophoretic mobility shift assay" +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000112 "Each of the promoter-proximal regions of qrr2 - 4 was subjected to EMSA with the purified His-OpaR protein (Fig. 6b). The results showed that His-OpaR was able to bind to each of the three DNA fragments tested in a dose-dependent manner in vitro." xsd:string {xref="PMID:22506036"} +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000097 +name: cDNA to DNA expression microarray evidence +alt_id: ECO:0001041 +alt_id: ECO:0005524 +def: "A type of expression microarray evidence where expression level is quantified by fluroescently-labeled cDNA (reverse transcribed from an unknown RNA sample) hybridized to DNA immobilized on a solid surface." [ECO:RCT] +synonym: "DNA microarray" RELATED [] +synonym: "RNA microarray" RELATED [] +is_a: ECO:0000058 ! expression microarray evidence +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000098 +name: obsolete in situ hybridization evidence +def: "A type of nucleic acid hybridization evidence based on localization of a specific segment of DNA or RNA by the application of a complementary strand of nucleic acid to which a reporter molecule is attached." [url:https\://www.ncbi.nlm.nih.gov/probe/docs/techish/] +comment: This term has been made obsolete with the clean up of experimental genomic evidence branch. +synonym: "in situ hybridization" RELATED [] +is_obsolete: true + +[Term] +id: ECO:0000100 +name: fractionation evidence +def: "A type of direct assay evidence resulting from the separation of various cell components while preserving their individual functions." [NBK:26936, url:https\://www.ncbi.nlm.nih.gov/books/NBK26936/] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000101 +name: Affymetrix GeneChip evidence +def: "A type of cRNA to DNA expression microarray evidence resulting from the use of a proprietary Affymetrix GeneChip System for analyzing complex genetic information." [ERO:0001265] +synonym: "Affymetrix array experiment evidence" EXACT [] +is_a: ECO:0000062 ! cRNA to DNA expression microarray evidence + +[Term] +id: ECO:0000102 +name: co-fractionation evidence +def: "A type of fractionation evidence resulting from a protein fractionated with other compounds, factors, or macromolecules." [TAIR:TED] +synonym: "co-fractionation" RELATED [] +is_a: ECO:0000100 ! fractionation evidence + +[Term] +id: ECO:0000104 +name: DNA to cDNA expression microarray evidence +def: "A type of expression microarray evidence where expression levels are quantified by hybridization of fluorescently-labeled DNA to cDNA (reverse transcribed from an unknown RNA sample) immobilized on a solid surface." [ECO:RCT, PMID:15329382] +comment: REM is a 'reverse-format' microarray, where the unknown sample is immoblized to a surface and known DNA is hybridized to that. {xref="PMID:15329382"} +synonym: "microarray RNA expression level evidence" EXACT [] +synonym: "REM evidence" EXACT [] +synonym: "RNA expression microarray evidence" EXACT [] +synonym: "transcript levels (e.g. microarray data)" RELATED [] +is_a: ECO:0000058 ! expression microarray evidence + +[Term] +id: ECO:0000105 +name: Nimblegen array evidence +def: "A type of cDNA to DNA expression microarray evidence resulting from the use of a proprietary NimbleGen array to measure expression levels." [PMID:15607417, url:https\://roche-biochem.jp/pdf/products/microarray/user_guide/SeqCap/SeqCap_UserGuide_Delivery_ver3.0.pdf] +is_a: ECO:0000097 ! cDNA to DNA expression microarray evidence + +[Term] +id: ECO:0000106 +name: northern blot evidence +def: "A type of transcript expression evidence based on electrophoresis and probing to determine levels of RNA expression using a complementary hybridization probe on the separated RNA samples." [ECO:SW, TAIR:TED] +synonym: "northern assay evidence" EXACT [] +synonym: "RNA blot evidence" EXACT [] +synonym: "transcript levels (e.g. Northerns)" RELATED [] +is_a: ECO:0000009 ! transcript expression evidence +property_value: IAO:0000112 "Northern blot analysis indicated that there was one major band of 1.5-kb (which was used for the stability determination) and two other minor bands of approximately 3.0- and 6.0-kb, which constituted less than 5% of the total signal (Fig. 1C), suggesting that other genes may be cotranscribed along with SMU.1882." xsd:string {xref="PMID:21124877"} +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000109 +name: reverse transcription polymerase chain reaction evidence +alt_id: ECO:0000108 +def: "A type of RNA detection assay evidence where an RNA transcript is reverse transcribed into cDNA and amplified to qualitatively detect gene expression." [ECO:SW, PMID:11013345, PMID:12901609] +comment: The starting product for PCR, and therefore amplification volume, is directly correlated to the transcription rate. +synonym: "reverse transcription polymerase chain reaction transcription evidence" BROAD [] +synonym: "RT-PCR" EXACT [] +synonym: "transcript levels (e.g. RT-PCR)" RELATED [] +is_a: ECO:0005518 ! RNA detection assay evidence +property_value: IAO:0000112 "Taken together, the results of the RT-PCR analyses and the reporter fusion assays indicate that expression of SMU.1882 is up regulated in the presence of CovR." xsd:string {xref="PMID:21124877"} +property_value: IAO:0000112 "The RT-PCR assay indicated that the sycO, ypkA and yopJ genes (designated as pCD12, pCD13 and pCD14 in Y. pestis 91001 [19], respectively) were transcribed as a single primary RNA (Fig. 1), and thereby these three genes constituted a single operon in Y. pestis Microtus strain 201." xsd:string {xref="PMID:19703315"} +property_value: IAO:0000112 "Using reverse transcription-PCR, we found that primers designed to span the intergenic regions between zur and the znuC homolog, as well as znuC and znuB homolog each generated a PCR product (Figure 2A). This indicated that these genes were transcribed on the same mRNA." xsd:string {xref="PMID:24086521"} +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000110 +name: RNA protection assay evidence +def: "A type of nuclease protection assay evidence where mRNA is hybridized with radiolabeled RNA probes after which RNAse is added to digest the unbound, nonresistant single-stranded overhang regions, later the remaining probe target hybrids are purified and resolved on denaturing polyacrylamide gel, and quantified by autoradiography." [ECO:SW, PMID:23457339, TAIR:TED] +synonym: "ribonuclease protection assay" EXACT [] +synonym: "RNA protection assay" RELATED [] +synonym: "RNAse protection assay" RELATED [] +synonym: "RPA" NARROW [] +is_a: ECO:0001106 ! nuclease protection assay evidence +property_value: IAO:0000112 "fur RPAs were performed on RNA isolated from each strain, and an increase in fur expression was seen for G27, 26695, and the Fur swap strain under iron-depletion shock conditions while little to no increase was seen under iron-limited growth conditions (Fig. 4D and data not shown). This data shows that Fur autoregulation is consistent in each strain and further supports the notion that the AA difference in Fur is not responsible for the difference in sodB regulation between G27 and 26695." xsd:string {xref="PMID:19399190"} +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000112 +name: qualitative western immunoblotting evidence +alt_id: ECO:0005602 +def: "A type of protein expression evidence where proteins are separated using gel electrophoresis, blotted onto a membrane, and probed with an antibody that can be detected via light or radioactive emission." [ECO:SW, PMID:23050259, TAIR:TED] +comment: Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies. +synonym: "protein expression level evidence based on western blot" EXACT [] +synonym: "protein immunoblot" RELATED [] +synonym: "protein levels (e.g. Western blots)" RELATED [] +synonym: "Western blot expression analysis" RELATED [] +is_a: ECO:0000010 ! protein expression evidence +is_a: ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence +is_a: ECO:0005593 ! immunodetection assay evidence +property_value: IAO:0000112 "When we determined the proteolysis role of Lons in VapBC10 proteins by Western blot analysis using the strains BL21(DE3)(pJS882) and BL21(DE3)(pJS427), the levels of VapB10 and VapC10 remained stable over the course of translation inhibition (Figure 7D and E). Thus, Lon could not degrade VapBC10 proteins, consistent with our drop growth evidence (Figure 6B)." xsd:string {xref="PMID:24260461"} +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000114 +name: expression library screen evidence +def: "A type of protein expression evidence where a protein of interest is isolated on the basis of its hybridization to an antibody raised to a homologous protein." [ECO:MCC, TAIR:TED] +synonym: "expression library screening" RELATED [] +is_a: ECO:0000010 ! protein expression evidence + +[Term] +id: ECO:0000116 +name: differential hybridization evidence +def: "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue is compared to another cell type or tissue when mRNA from each sample is reverse transcribed, labeled, and hybridized to a cDNA library to compare hybridization patterns." [PMID:14668817] +comment: Differential hybridization identifies differentially expressed cloned genes. Two different mRNA-derived probes are used to demonstrate RNA expression under a specific condition. +synonym: "differential hybridization" RELATED [] +is_a: ECO:0000009 ! transcript expression evidence + +[Term] +id: ECO:0000118 +name: subtractive hybridization evidence +def: "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue are compared to another cell type or tissue when denatured ds-cDNA from the two samples are hybridized, and the common sequences are 'subtracted'." [PMID:11298187] +comment: Subtractive hybridization is used for isolation and identification of transcripts involving the removal of any sequences present in the control from the test probe to detect only transcripts present in the sample. The method is PCR-based for the comparison of two cDNA libraries. +synonym: "subtractive hybridization" RELATED [] +is_a: ECO:0000009 ! transcript expression evidence + +[Term] +id: ECO:0000120 +name: over expression analysis evidence +def: "A type of experimental phenotypic evidence in which a gene and/or gene product is investigated in a transgenic organism that has been engineered to overexpress that gene product." [PMID:22419077] +synonym: "analysis of overexpression/ectopic expression phenotype" RELATED [] +is_a: ECO:0005027 ! genetic transformation evidence +property_value: IAO:0000112 "In contrast, when RpoQ was overexpressed in either the wild type or the DeltalitR mutant, both were essentially nonmotile (Fig. 7), suggesting that quorum signaling regulates motility, at least in part, through an RpoQ mechanism downstream of LitR." xsd:string {xref="PMID:22233679"} + +[Term] +id: ECO:0000122 +name: protein localization evidence +def: "A type of localization evidence where sub-cellular localization of a protein is determined." [GO:0008104, url:http\://journals.plos.org/plosone/article?id=10.1371/journal.pone.0019937] +is_a: ECO:0001533 ! localization evidence + +[Term] +id: ECO:0000124 +name: fusion protein localization evidence +def: "A type of protein localization evidence resulting from the fusion of a protein of interest to a labeling protein which has enzymatic activity or fluorescence properties." [MI:0240] +is_a: ECO:0000122 ! protein localization evidence + +[Term] +id: ECO:0000126 +name: green fluorescent protein fusion protein localization evidence +def: "A type of fusion protein localization evidence resulting from the labeling of a protein of interest with green fluorescent protein (GFP) from the jellyfish Aequorea victoria." [PMID:9759496] +synonym: "GFP fusion protein localization evidence" EXACT [] +synonym: "localization of GFP/YFP fusion protein" RELATED [] +is_a: ECO:0000124 ! fusion protein localization evidence + +[Term] +id: ECO:0000128 +name: yellow fluorescent protein fusion protein localization evidence +def: "A type of fusion protein localization evidence resulting from the labeling of a protein of interest with yellow fluorescent protein (YFP), which was created by mutating green fluorescent protein (GFP) of the Aequorea victoria." [MI:0368, url:http\://www.jbc.org/content/276/31/29188.long] +synonym: "localization of GFP/YFP fusion protein" RELATED [] +synonym: "YFP fusion protein localization evidence" EXACT [] +is_a: ECO:0000124 ! fusion protein localization evidence + +[Term] +id: ECO:0000130 +name: beta-glucuronidase fusion protein localization evidence +def: "A type of fusion protein localization evidence resulting from the targeted insertion of the beta-glucuronidase (GUS) coding gene as a reporter gene for the localization of a particular gene product." [ECO:RCT] +synonym: "GUS fusion protein localization evidence" EXACT [] +synonym: "GUS staining evidence" NARROW [] +synonym: "localization of GUS fusion protein" RELATED [] +is_a: ECO:0000124 ! fusion protein localization evidence + +[Term] +id: ECO:0000132 +name: beta-galactosidase fusion protein localization evidence +def: "A type of fusion protein localization evidence resulting from the targeted insertion of the beta-galactosidase coding gene (lacZ) into a specific gene to create a beta-galactosidase-tagged fusion protein." [PMID:23681629] +synonym: "LacZ fusion protein localization evidence" EXACT [] +is_a: ECO:0000124 ! fusion protein localization evidence + +[Term] +id: ECO:0000134 +name: transport assay evidence +def: "A type of direct assay evidence resulting from the activity assessment of transport proteins." [PMID:18401524] +synonym: "transport assay" RELATED [] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000136 +name: nucleic acid binding evidence +def: "A type of affinity evidence resulting from the binding of a molecule to a nucleic acid." [GO:0003676] +is_a: ECO:0000023 ! affinity evidence + +[Term] +id: ECO:0000138 +name: ribohomopolymer binding assay evidence +def: "A type of nucleic acid binding evidence resulting from an enzyme displaying binding activity to specific ribohomopolymer." [PMC:102612] +synonym: "ribohomopolymer binding assay" RELATED [] +is_a: ECO:0000136 ! nucleic acid binding evidence + +[Term] +id: ECO:0000140 +name: thin layer chromatography evidence +def: "A type of chromatography evidence that uses a thin layer of adsorbent (such as silica gel, alumina, or cellulose) on a flat, inert substrate." [ECO:MCC] +synonym: "thin layer chromatography" RELATED [] +synonym: "TLC evidence" EXACT [] +is_a: ECO:0000325 ! chromatography evidence + +[Term] +id: ECO:0000142 +name: protein:ion binding evidence +def: "A type of protein binding evidence resulting from a metal ion binding to a protein at a specific binding site." [PMID:2377604] +is_a: ECO:0000024 ! protein binding evidence + +[Term] +id: ECO:0000144 +name: Southwestern blot evidence +def: "A type of nucleic acid binding evidence in which DNA-protein binding is detected using labeled DNA as probes, hybridized to electrophoretically separated proteins." [ECO:RCT, TAIR:TED] +synonym: "Southwestern analysis" RELATED [] +is_a: ECO:0000136 ! nucleic acid binding evidence + +[Term] +id: ECO:0000146 +name: Northwestern blot evidence +def: "A type of nucleic acid binding evidence in which RNA-protein binding is detected using labeled RNA as probes, hybridized to electrophoretically separated proteins." [ECO:RCT, TAIR:TED] +synonym: "Northwestern analysis" RELATED [] +is_a: ECO:0000136 ! nucleic acid binding evidence + +[Term] +id: ECO:0000148 +name: in vitro binding evidence +is_obsolete: true + +[Term] +id: ECO:0000150 +name: in vitro transcription reconstitution assay evidence +def: "A type of reconstitution assay evidence resulting from the use of reconstituted polymerase transcription systems to investigate RNA synthesis." [PMID:9237163] +synonym: "in vitro reconstitution assay with recombinant protein" RELATED [] +is_a: ECO:0000003 ! reconstitution assay evidence +property_value: IAO:0000112 "As shown in Fig. 5, addition of reconstituted RNA polymerase holoenzyme, but not the core enzyme, generated expected size bands of 193-nt and 158-nt when P1882 and Pami, respectively, were used as template (lanes 2 and 7). However, the addition of CovR did not lead to any observable increase in transcription from P1882." xsd:string {xref="PMID:21124877"} + +[Term] +id: ECO:0000152 +name: in vitro recombinant protein transcription reconstitution assay evidence +def: "A type of in vitro transcription reconstitution assay evidence resulting from the use of recombinant proteins in preparation of a fully-defined transcription system." [PMID:9237165] +synonym: "in vitro reconstitution assay with recombinant protein" RELATED [] +is_a: ECO:0000150 ! in vitro transcription reconstitution assay evidence + +[Term] +id: ECO:0000154 +name: heterologous protein expression evidence +def: "A type of protein expression evidence where a gene from one cell is inserted into a cell that does not typically contain that gene and heterologous protein expression is assessed." [ECO:KAV] +synonym: "protein expression in heterologous system" RELATED [] +is_a: ECO:0000010 ! protein expression evidence + +[Term] +id: ECO:0000156 +name: protein separation evidence +def: "A type of fractionation evidence resulting from the isolation of a single type of protein from a complex mixture." [ERO:0000526] +is_a: ECO:0000100 ! fractionation evidence + +[Term] +id: ECO:0000158 +name: protein separation followed by direct sequencing evidence +def: "A type of protein separation evidence that is followed by direct protein sequencing whereby a protein's amino acid sequence is determined experimentally by Edman degradation or by mass spectrometry." [] +synonym: "protein separation and direct sequencing" RELATED [] +is_a: ECO:0000156 ! protein separation evidence + +[Term] +id: ECO:0000160 +name: protein separation followed by fragment identification evidence +def: "A type of protein separation evidence which then utilizes the screening and identification of small-molecules that bind to proteins." [https://pubs.acs.org/doi/10.1021/bi3005126, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5632530/] +synonym: "protein separation and fragment identification" RELATED [] +is_a: ECO:0000156 ! protein separation evidence + +[Term] +id: ECO:0000162 +name: heterologous system uptake evidence +def: "A type of transport assay evidence in which the uptake mechanism of a transporter is investigated in a cell that does not normally express that protein." [doi:10.1146/annurev.pp.46.060195.002223, ECO:RCT] +comment: The transporter is a recombinant protein. +synonym: "uptake assay in heterologous system" RELATED [] +is_a: ECO:0000134 ! transport assay evidence + +[Term] +id: ECO:0000164 +name: electrophysiology assay evidence +def: "A type of direct assay evidence where electrical properties of cells or tissues are studied." [ECO:KAV] +comment: The scale of electrophysiology assays can range from small, e.g. a single ion channel, to large, e.g. an entire organ such as a heart. +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000166 +name: two-electrode voltage clamp recording evidence +def: "A type of voltage clamp recording evidence which involves using two electrodes inserted into a large cell (often a xenopus oocyte) where one electrode is used to measure the internal potential (voltage) of the cell and the other electrode is used to clamp the current." [ECO:SN, PMID:23529422] +synonym: "two-electrode voltage clamp technique" RELATED [] +is_a: ECO:0005576 ! voltage clamp recording evidence + +[Term] +id: ECO:0000168 +name: transcription assay evidence +def: "A type of direct assay evidence resulting from the detection and quantification of transcribed RNA." [MeSH:D014158, url:https\://bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-15-7] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000170 +name: transcriptional activation assay evidence +def: "A type of transcription assay evidence in which sequence-specific binding proteins that increase transcription of a specific target gene or genes are identified, quantified, and/or analyzed." [url:http\://smcg.ccg.unam.mx/enp-unam/05-TranscripYRegulacion/transcription.pdf] +synonym: "transcriptional activation assay" RELATED [] +is_a: ECO:0000168 ! transcription assay evidence + +[Term] +id: ECO:0000172 +name: biochemical trait analysis evidence +def: "A type of experimental phenotypic evidence in which the biochemical aspect of a mutation is analyzed." [ECO:RCT] +comment: For example, the accumulation of a biosynthetic intermediate. +synonym: "analysis of biochemical trait" RELATED [] +is_a: ECO:0000059 ! experimental phenotypic evidence + +[Term] +id: ECO:0000174 +name: mutant physiological response evidence +def: "A type of mutant phenotype evidence in which the physiological response of a mutant when presented with an external stimulus is determined." [ECO:RCT] +comment: Examples include: abnormal growth of the root in response to gravity, delay in flowering in response to varying light conditions. +synonym: "analysis of physiological response" RELATED [] +is_a: ECO:0000015 ! mutant phenotype evidence + +[Term] +id: ECO:0000176 +name: mutant visible phenotype evidence +def: "A type of mutant phenotype evidence in which traits arising from a mutation are visually examined." [ECO:RCT] +synonym: "analysis of visible trait" RELATED [] +is_a: ECO:0000015 ! mutant phenotype evidence + +[Term] +id: ECO:0000177 +name: genomic context evidence +def: "A type of similarity evidence in which the location of the gene within the genome provides insight into the gene's function or evolutionary insight." [PMID:10958625, PMID:21609955] +comment: This type of evidence might include identity of neighboring genes, operon structure, synteny, phylogenetic analysis, or other whole-genome analysis. +is_a: ECO:0000041 ! similarity evidence + +[Term] +id: ECO:0000178 +name: in vivo assay evidence +def: "A type of direct assay evidence resulting from a sample of microorganisms or living tissue grown in a medium." [ECO:RCT, url:https\://www.cancer.gov/publications/dictionaries/cancer-terms?cdrid=46352] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000179 +name: animal model system study evidence +def: "A type of experimental evidence arising from the investigation of an animal model system." [ECO:MCC] +is_a: ECO:0000006 ! experimental evidence + +[Term] +id: ECO:0000180 +name: clinical study evidence +def: "A type of experimental evidence arising from a controlled investigation that uses human subjects." [ECO:MCC] +is_a: ECO:0000006 ! experimental evidence + +[Term] +id: ECO:0000181 +name: in vitro assay evidence +alt_id: ECO:0005013 +def: "A type of direct assay evidence resulting from a process performed outside the living organism, where the components of an organism have been isolated from their usual biological context, to permit a more detailed or more convenient analysis in an artificial setting." [ECO:RCT, url:https\://www.cancer.gov/publications/dictionaries/cancer-terms?cdrid=45733] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000182 +name: in vitro culture assay evidence +comment: Use ECO:0001563 (cell growth assay evidence) in place of this term. +is_obsolete: true + +[Term] +id: ECO:0000183 +name: cell-free assay evidence +def: "A type of direct assay evidence resulting from studies in which cytoplasmic and/or nuclear cellular components from resting, nonstimulated cells, are isolated by ultracentrifugation to provide molecular machinery that can be used in reactions in the absence of many of the other cellular components for the purpose of in vitro protein synthesis, transcription, DNA replication, etc." [PMID:18453125] +synonym: "in vitro assay" BROAD [] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000184 +name: enzyme inhibition evidence +def: "A type of protein inhibition evidence and enzymatic acitivty evidence where the protein under study is an enzyme." [ECO:MCC] +is_a: ECO:0000005 ! enzymatic activity assay evidence +is_a: ECO:0000020 ! protein inhibition evidence + +[Term] +id: ECO:0000200 +name: sequence alignment evidence +alt_id: ECO:00000057 +def: "A type of sequence similarity evidence in which an annotation is made based on a manually-reviewed or published sequence alignment" [url:http\://www.geneontology.org/GO.evidence.shtml] +comment: Such alignments may be pairwise alignments or multiple alignments. +is_a: ECO:0000044 ! sequence similarity evidence +created_by: mchibucos +creation_date: 2010-03-18T12:21:31Z + +[Term] +id: ECO:0000201 +name: sequence orthology evidence +alt_id: ECO:00000060 +def: "A type of sequence similarity evidence in which orthology is established by multiple criteria, including amino acid and/or nucleotide sequence comparisons and one or more of the following: phylogenetic analysis, coincident expression, conserved map location, functional complementation, immunological cross-reaction, similarity in subcellular localization, subunit structure, substrate specificity, and response to specific inhibitors." [url:http\://www.geneontology.org/GO.evidence.shtml] +comment: Orthology is a relationship between genes in different species indicating that the genes derive from a common ancestor. +synonym: "ortholog evidence" RELATED [] +is_a: ECO:0000044 ! sequence similarity evidence +created_by: mchibucos +creation_date: 2010-03-18T12:30:06Z + +[Term] +id: ECO:0000202 +name: match to sequence model evidence +alt_id: ECO:00000063 +def: "A type of sequence similarity evidence in which the function of a gene product is predicted based on a sequence-based statistical model." [ECO:MCC] {comment="url:http://www.geneontology.org/GO.evidence.shtml"} +comment: Used when evidence from any kind of statistical model of a sequence or group of sequences is used to make a prediction about the function of a protein or RNA. Examples of relevant evidence are: Hidden Markov Models, PROSITE motifs, and tools such as tRNASCAN. +is_a: ECO:0000044 ! sequence similarity evidence +property_value: IAO:0000112 "A putative rho-independent terminator sequence (GCCTGACTACATAGATGTCAGGC) was identified at position 402-bp downstream of SMU.1882 by TransTerm (transterm.dev.java.net) program." xsd:string {xref="PMID:21124877"} +created_by: mchibucos +creation_date: 2010-03-18T12:32:30Z + +[Term] +id: ECO:0000203 +name: automatic assertion +alt_id: ECO:00000067 +def: "An assertion method that does not involve human review." [ECO:MCC] +comment: An automatic assertion is based on computationally generated information that is not reviewed by a person prior to making the assertion. For example, one common type of automatic assertion involves creating an association of evidence with an entity based on commonness of attributes of that entity and another entity for which an assertion already exists. The commonness is determined algorithmically. +is_a: ECO:0000217 ! assertion method +disjoint_from: ECO:0000218 ! manual assertion +created_by: mchibucos +creation_date: 2010-03-18T12:36:04Z + +[Term] +id: ECO:0000204 +name: author statement +alt_id: ECO:0007010 +def: "A type of documented statement evidence that is based on an assertion by the author of a paper, which is read by a curator." [ECO:MCC] +is_a: ECO:0006151 ! documented statement evidence +created_by: mchibucos +creation_date: 2010-06-21T11:17:21Z + +[Term] +id: ECO:0000205 +name: curator inference +def: "A type of inferential evidence that is based on a conclusion drawn by a curator." [ECO:MCC] +is_a: ECO:0000361 ! inferential evidence +created_by: mchibucos +creation_date: 2010-08-18T05:55:12Z + +[Term] +id: ECO:0000206 +name: BLAST evidence +def: "A type of pairwise sequence alignment evidence obtained with basic local alignment search tool (BLAST)." [ECO:MCC] +is_a: ECO:0005554 ! pairwise sequence alignment evidence +created_by: mchibucos +creation_date: 2010-08-06T04:48:24Z + +[Term] +id: ECO:0000207 +name: nucleotide BLAST evidence +def: "A type of BLAST evidence in which nucleotide sequences are aligned." [ECO:MCC] +is_a: ECO:0000206 ! BLAST evidence +created_by: mchibucos +creation_date: 2010-08-06T04:49:58Z + +[Term] +id: ECO:0000208 +name: protein BLAST evidence +def: "A type of BLAST evidence in which amino acid or translated nucleotide sequences are aligned." [ECO:MCC] +is_a: ECO:0000206 ! BLAST evidence +property_value: IAO:0000112 "Blast search of S. meliloti genome for homologues of X. campestris Ohr protein revealed two paralogues, SMa2389 and SMc00040, showing 52 and 57% identity respectively with Ohr of X. campestris." xsd:string {xref="PMID:21569462"} +created_by: mchibucos +creation_date: 2010-08-06T04:50:45Z + +[Term] +id: ECO:0000209 +name: BLAST evidence used in automatic assertion +def: "A type of BLAST evidence that is used in an automatic assertion." [ECO:MCC] +is_a: ECO:0000206 ! BLAST evidence +is_a: ECO:0005561 ! pairwise sequence alignment evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-08-06T04:55:21Z + +[Term] +id: ECO:0000210 +name: nucleotide BLAST evidence used in automatic assertion +def: "A type of nucleotide BLAST evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000207 ! nucleotide BLAST evidence +is_a: ECO:0000209 ! BLAST evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-08-06T05:09:51Z + +[Term] +id: ECO:0000211 +name: protein BLAST evidence used in automatic assertion +def: "A type of protein BLAST evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000208 ! protein BLAST evidence +is_a: ECO:0000209 ! BLAST evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-08-06T05:11:04Z + +[Term] +id: ECO:0000212 +name: combinatorial evidence +alt_id: ECO:0000036 +alt_id: ECO:0000043 +def: "A type of evidence in which at least two distinct types of evidence have been integrated." [ECO:MCC, ECO:RCT] +comment: A key aspect of this type of evidence is that two or more pieces of information are combined to generate an emergent type of evidence not possible with the constituent pieces of evidence alone. A combinatorial analysis typically involves incorporation of different types of evidence. +synonym: "inferred from in-silico analysis" RELATED [] +is_a: ECO:0000000 ! evidence +created_by: mchibucos +creation_date: 2010-08-06T05:24:57Z + +[Term] +id: ECO:0000213 +name: combinatorial evidence used in automatic assertion +def: "A type of combinatorial analysis that is used in an automatic assertion." [ECO:MCC] +comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments. +synonym: "inferred from in-silico analysis" RELATED [] +is_a: ECO:0000212 ! combinatorial evidence +is_a: ECO:0000501 ! evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-08-06T05:28:33Z + +[Term] +id: ECO:0000214 +name: biological aspect of descendant evidence +def: "A type of phylogenetic evidence whereby an aspect of an ancestral gene is inferred through the characterization of an aspect of a descendant gene." [ECO:MCC] +comment: This type of evidence can be used in support of both positive and "not" GO annotations. It should be used to annotate ancestral genes but not extant ones. +is_a: ECO:0000080 ! phylogenetic evidence +created_by: mchibucos +creation_date: 2010-10-05T11:16:50Z + +[Term] +id: ECO:0000215 +name: rapid divergence from ancestral sequence evidence +def: "A type of phylogenetic evidence characterized by long phylogenetic tree branch lengths following a duplication event." [ECO:MCC] +comment: Long branch lengths indicate that descendant sequences may have acquired different or additional functions than the ancestral sequence. +is_a: ECO:0000080 ! phylogenetic evidence +created_by: mchibucos +creation_date: 2010-10-05T03:41:34Z + +[Term] +id: ECO:0000216 +name: phylogenetic determination of loss of key residues evidence +def: "A type of phylogenetic evidence characterized by the absence of key sequence residues." [ECO:MCC] +comment: The loss of certain residues important for function can indicate that a sequence lacks a particular function. +is_a: ECO:0000080 ! phylogenetic evidence +created_by: mchibucos +creation_date: 2010-10-05T03:43:17Z + +[Term] +id: ECO:0000217 +name: assertion method +def: "A means by which a statement is made about an entity." [ECO:MCC] +created_by: mchibucos +creation_date: 2010-11-12T04:17:37Z + +[Term] +id: ECO:0000218 +name: manual assertion +def: "An assertion method that involves human review." [ECO:MCC] +comment: A manual assertion could be based on evidence that is generated by and interpreted by a human or it could involve human review of computationally generated information. +is_a: ECO:0000217 ! assertion method +created_by: mchibucos +creation_date: 2010-11-12T04:20:02Z + +[Term] +id: ECO:0000219 +name: nucleotide sequencing assay evidence +def: "A type of sequencing assay that determines the sequence of a biopolymer comprised of nucleotides." [ECO:MCC] +synonym: "DNA sequencing" RELATED [OBI:0000626] +is_a: ECO:0000220 ! sequencing assay evidence +created_by: mchibucos +creation_date: 2010-11-15T12:22:40Z + +[Term] +id: ECO:0000220 +name: sequencing assay evidence +def: "A type of experimental evidence where the order of molecules that constitute a biopolymer is determined." [ECO:MCC, OBI:0600047] +is_a: ECO:0000006 ! experimental evidence +created_by: mchibucos +creation_date: 2010-11-15T01:44:45Z + +[Term] +id: ECO:0000221 +name: high throughput nucleotide sequencing assay evidence +def: "A type of nucleotide sequencing assay evidence in which a sequence is derived from a parallelized sequencing technique." [ECO:MCC] +comment: Typically thousands to millions of reads are produced in parallel. +synonym: "next generation sequencing evidence" RELATED [] +is_a: ECO:0000219 ! nucleotide sequencing assay evidence +created_by: mchibucos +creation_date: 2010-11-15T02:19:34Z + +[Term] +id: ECO:0000222 +name: Illumina sequencing evidence +def: "A type of high throughput nucleotide sequencing evidence in which sequence is determined through hybridization of adaptor-ligated DNA to a glass slide on which each fragment is simultaneously clonally amplified, followed by progressive rounds of base incorporation with fluorescently-tagged nucleotides, washing, imaging, and cleavage." [OBI:0000724, PMID:26000844] +comment: In Illumina sequencing technology, adapters are ligated onto the ends of randomly fragmented DNA. After single-stranded fragments are bound to the inside surface of a flow cell channel, they undergo in-place bridge PCR amplification. To begin the sequencing cycle, labeled reversible terminators, primers and DNA polymerase are added, followed by laser excitation of the fluorescent label and high resolution scanning. After non-incorporated nucleotides are washed away and the dye is chemically removed, the next cycle repeats with four labeled reversible terminators, primers and DNA polymerase, again followed by imaging. +synonym: "Solexa sequencing result" RELATED [OBI:0000724] +is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence +created_by: mchibucos +creation_date: 2010-11-15T02:38:03Z + +[Term] +id: ECO:0000223 +name: 454 pyrosequencing evidence +def: "A type of high throughput nucleotide sequencing evidence in which the sequence for a ssDNA is determined by addition of primers and then nucleotides, one base pair at a time (which generate a specific fluorescence), to the target strand." [EFO:0005016] +comment: 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA.\n\nThe target strand is usually prepared by fragmentation, adaptor ligation, and amplification by emulsion PCR. +synonym: "pyrosequencing" BROAD [OBI:0000730] +is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence +created_by: mchibucos +creation_date: 2010-11-15T03:50:14Z + +[Term] +id: ECO:0000224 +name: SOLiD sequencing evidence +def: "A type of high throughput nucleotide sequencing evidence in which sequence is determined through rounds of ligation-based sequencing with fluorescently-labeled Di-base probes and cleavage, after which the template is reset, with a primer offset by one base, for subsequent rounds of ligation." [OBI:0000706] +comment: SOLiD sequencing technology is based on sequencing by ligation. After library preparation, emulsion PCR and bead enrichment are performed, including 3' modification of templates on selected beads to allow covalent bonding to a slide. 3' modified beads are attached to a glass slide and primers are added. Four fluorescently labeled di-base probes are added and multiple cycles of ligation, detection and cleavage are performed. The number of cycles determines read length. After a number of ligation cycles, the extension product is removed and another round of ligation cycles is performed with a new primer complimentary to the n-1 position of the template. Five rounds of primer reset are performed for each sequence tag.\n\nSamples are prepared by fragmentation of a sample library, adaptor ligation, emulsion PCR, and attachment of resultant beads to glass slides. +is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence +created_by: mchibucos +creation_date: 2010-11-15T04:17:41Z + +[Term] +id: ECO:0000225 +name: chain termination sequencing evidence +def: "A type of nucleotide sequencing assay in which DNA sequence is determined by addition of a primed DNA template to a reaction mixture, followed by addition of DNA polymerase, deoxynucleosidetriphosphates (dNTPs - one of which may be radiolabeled), and one of four di-deoxynucleotidetriphosphates (ddNTPs), after which DNA elongation is terminated and the DNA is separated by size and imaged." [OBI:0000695] +comment: Chain termination sequencing, also called Sanger sequencing after its developer, uses dideoxynucleotide triphosphates (ddNTPs) as DNA chain terminators. Single-stranded DNA template, DNA primer, DNA polymerase, fluorescently or radioactively labeled nucleotides, and one of four ddNTPs are added together in each of four separate sequencing reactions. The synthesized and labeled DNA fragments from each of the four reactions are denatured, separated by size by gel electrophoresis, and visualized by autoradiography or UV light.\n\nThe ddNTPs lack an OH group so the next dNTP cannot be attached, causing chain termination. +synonym: "dye terminator sequencing" EXACT [] +synonym: "Sanger sequencing" EXACT [] +is_a: ECO:0000219 ! nucleotide sequencing assay evidence +created_by: mchibucos +creation_date: 2010-11-15T04:46:31Z + +[Term] +id: ECO:0000226 +name: chromatin immunoprecipitation evidence +def: "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC] +comment: Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq). +synonym: "ChIP evidence" EXACT [] +xref: MI:0402 "chromatin immunoprecipitation assay" +is_a: ECO:0000085 ! immunoprecipitation evidence +created_by: mchibucos +creation_date: 2010-11-16T01:29:08Z + +[Term] +id: ECO:0000227 +name: chromatin immunoprecipitation-PCR evidence +def: "A type of chromatin immunoprecipitation evidence that uses polymerase chain reaction (PCR) where specific primers are used on the pulled-down DNA for DNA detection." [ECO:MCC, ECO:SW, PMID:18388953] +comment: ChIP-PCR is often used to validate ChIP-chip results and less frequently,ChIP-Seq results. +synonym: "ChIP-PCR evidence" EXACT [] +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: mchibucos +creation_date: 2010-11-16T05:02:00Z + +[Term] +id: ECO:0000228 +name: chromatin immunoprecipitation-qPCR evidence +def: "A type of chromatin immunoprecipitation evidence in which protein-DNA interactions are investigated at known genomic binding sites through a process of ChIP, DNA purification, and finally quantitative polymerase chain reaction (qPCR) for DNA detection." [url:https\://www.diagenode.com/applications/chip-qpcr] +synonym: "ChIP-qPCR evidence" EXACT [] +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +property_value: IAO:0000112 "To confirm the in vivo binding of FimR to pfim, and to determine the impact of Mn2+ and Fe2+ on the binding activity of FimR, chromatin immunoprecipitation (ChIP) assay-quantitative real time PCR (qPCR) with anti-FimR antibody was employed as detailed in the materials and methods. The strongest binding of FimR to pfim was detected in cells grown in the presence of 50 microM MnCl2 and 50 microM FeSO4 (Figure 5, lane IV), whereas minimal amounts of MnCl2 (0.01 microM) and FeSO4 (0.1 microM) led to the weakest binding (Figure 5, lane I)." xsd:string {xref="PMID:23823757"} +created_by: mchibucos +creation_date: 2010-11-16T05:26:47Z + +[Term] +id: ECO:0000229 +name: chromatin immunoprecipitation-seq evidence +def: "A type of chromatin immunoprecipitation evidence that uses high-throughput sequencing where immunoprecipitated DNA fragments are funnelled into a massively parallel sequencer to produce multiple short reads for locating binding sites of DNA-associated proteins." [ECO:MCC, ECO:SW, OBI:0000716, PMID:22955991] +comment: ChIP-Seq experiments often use the input as a control to eliminate biases. To obtain similar results, ChIP-chip with high-density tiling arrays can be used. +synonym: "ChIP-SEQ evidence" EXACT [OBI:0000716] +synonym: "ChIP-seq evidence" EXACT [PMID:22955991] +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: mchibucos +creation_date: 2010-11-16T05:30:41Z + +[Term] +id: ECO:0000230 +name: chromatin immunoprecipitation-chip evidence +def: "A type of chromatin immunoprecipitation evidence that uses a tiling microarray for the detection of protein-bound DNA regions where chromatin is immunoprecipitated for protein tagging and DNA is sheared from the cross-linked protein-DNA complex to be arrayed revealing the protein-bound genomic regions." [ECO:MCC, ECO:SW, PMID:18388953, PMID:19381927] +comment: A fixating agent such as formaldehyde is used to cross link proteins and DNA. Sonication is applied for DNA shearing. For transcription factor tagging, an antibody or epitope is employed. After cross-linking is reversed, the DNA is amplified and labeled with a flourophore for microarray which produces a resolution of aproximately 500 bp. +synonym: "ChIP-chip evidence" EXACT [] +synonym: "ChIP-on-chip evidence" EXACT [] +xref: MI:0225 "chromatin immunoprecipitation array" +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: mchibucos +creation_date: 2010-11-16T05:43:55Z + +[Term] +id: ECO:0000231 +name: quantitative polymerase chain reaction evidence +def: "A type of DNA detection assay evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes or fluorescent probes for detection and quantification of specific sequences in a DNA sample." [ECO:MCC, ECO:SW] +comment: Quantitative PCR enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. The dye is activated upon binding double-stranded DNA.\n\nAlthough qRT-PCR is commonly found in literature synonymous to qPCR, it is preferable not to use this synonym as it is the accepted abbreviation for reverse transcription PCR. qPCR should be used instead (ISBN 978-0-470-68386-6 page 173).\n\nqPCR can be used to quantitatively determine levels of gene expression. +synonym: "Q-PCR evidence" EXACT [] +synonym: "qPCR evidence" EXACT [] +synonym: "qRT-PCR evidence" RELATED [] +synonym: "quantitative PCR evidence" EXACT [] +synonym: "quantitative real-time PCR evidence" EXACT [] +synonym: "real time polymerase chain reaction evidence" EXACT [] +synonym: "real-time PCR evidence" EXACT [] +synonym: "real-time quantitative PCR" EXACT [] +is_a: ECO:0005519 ! DNA detection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: mchibucos +creation_date: 2010-11-16T05:57:20Z + +[Term] +id: ECO:0000232 +name: chromosome conformation-based evidence +def: "A type of experimental evidence that is based on a five-step technique of cross-linking DNA in vivo, restriction digestion, intramolecular ligation, reversing DNA cross-links, DNA processing, and quantitation that serves to analyze the spatial organization of chromatin in a cell and quantify the number of interactions between genomic loci that are nearby in 3-D space." [ECO:MCC] +comment: Chromosome conformation capture technologies are used to study the structural properties and spatial organization of chromosomes inside cells. All types of chromosome conformation capture technology involve five primary steps: cross-linking of DNA in vivo, restriction digestion, intramolecular ligation, reversing cross-linkages, and DNA enrichment and quantitation. The last step is different for each type of chromosome conformation capture technology, and may include PCR (3C), inverse PCR (4C), or ligation-mediated amplification (5C). Methods for product quantitation can include agarose gel detection or real-time quantitative PCR in the case of 3C, or high-throughput sequencing or microarray analysis for 4C and 5C. +synonym: "3C" NARROW [] +synonym: "chromosome conformation capture" NARROW [] +synonym: "chromosome conformation capture-based evidence" EXACT [] +is_a: ECO:0000006 ! experimental evidence +created_by: mchibucos +creation_date: 2010-11-17T12:27:14Z + +[Term] +id: ECO:0000233 +name: 3C evidence +def: "A type of chromosome conformation-based evidence that is derived by 1) quantifying interactions between a single pair of genomic loci and not multiple interactions and 2) performing polymerase chain reaction (PCR) in the final product quantitation step." [ECO:MCC] +comment: 3C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C differs only in the fifth step, which involves performing either standard polymerase chain reaction (PCR) or quantitative PCR (qPCR) for product quantitation. +synonym: "3C" EXACT [] +synonym: "chromosome conformation capture evidence" EXACT [] +synonym: "chromosome conformation capture-PCR evidence" NARROW [] +is_a: ECO:0000232 ! chromosome conformation-based evidence +created_by: mchibucos +creation_date: 2010-11-17T12:46:15Z + +[Term] +id: ECO:0000234 +name: 4C evidence +def: "A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray." [ECO:MCC, PMID:17033624] +comment: 4C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 4C differs only in the fifth step, which involves inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation. +synonym: "chromosome conformation capture on chip" EXACT [] +synonym: "circularized 3C" EXACT [] +synonym: "circularized chromosome conformation capture evidence" EXACT [] +is_a: ECO:0000232 ! chromosome conformation-based evidence +created_by: mchibucos +creation_date: 2010-11-19T01:20:28Z + +[Term] +id: ECO:0000235 +name: 5C evidence +def: "A type of chromosome conformation-based evidence that is derived by performing ligation-mediated amplification (LMA) prior to product quantitation." [ECO:MCC] +comment: 5C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 5C differs only in the fifth step, which involves ligation-mediated amplification (LMA) followed by product quantitation. +synonym: "5C" EXACT [] +synonym: "carbon-copy 3C" EXACT [] +synonym: "carbon-copy chromosome conformation capture evidence" EXACT [] +is_a: ECO:0000232 ! chromosome conformation-based evidence +created_by: mchibucos +creation_date: 2010-11-19T01:22:20Z + +[Term] +id: ECO:0000236 +name: chromosome conformation capture-PCR evidence +def: "A type of chromosome conformation capture evidence that is derived by performing polymerase chain reaction (PCR) in the quantitation step." [ECO:MCC] +comment: See 3C evidence. 3C evidence was initially developed using PCR. +synonym: "3C" BROAD [] +synonym: "3C-PCR" EXACT [] +is_obsolete: true +created_by: mchibucos +creation_date: 2010-11-19T03:00:47Z + +[Term] +id: ECO:0000237 +name: 3C-qPCR evidence +def: "A type of 3C evidence evidence that 1) quantifies interactions between a single pair of genomic loci (not multiple interactions) and 2) performs quantitative polymerase chain reaction (qPCR) in the quantitation step." [ECO:MCC, PMID:17641637, PMID:26025624] +synonym: "3C-quantitative PCR" EXACT [] +synonym: "3C-real-time PCR" EXACT [] +synonym: "chromosome conformation capture-qPCR evidence" EXACT [] +is_a: ECO:0000233 ! 3C evidence +created_by: mchibucos +creation_date: 2010-11-19T03:06:47Z + +[Term] +id: ECO:0000238 +name: Hi-C evidence +def: "A type of chromosome conformation-based evidence that is derived by performing immunoprecipitation enrichment of the biotin labelled ligated fragments events prior to sequencing of the library and mapping it to the genome." [ECO:MCC] +comment: Hi-C is used to study the structural properties and spatial organization of chromosomes inside cells. Hi-C is similar to other types of chromosome conformation capture technology, but involves the addition of biotinylated nucleotides during the second or fourth step and enrichment for ligated fragments using immunoprecipitation after the fourth step. +is_a: ECO:0000232 ! chromosome conformation-based evidence +created_by: mchibucos +creation_date: 2010-11-19T04:24:03Z + +[Term] +id: ECO:0000239 +name: 3C-seq evidence +def: "A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with multiplexed high-throughput (HT) sequencing." [ECO:MCC, PMID:23411633] +comment: 3C-seq is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C-seq differs only in the fifth step, which involves inverse polymerase chain reaction (PCR) on a circularized 3C library prior to product quantitation. +synonym: "3C sequencing" EXACT [] +synonym: "chromosome conformation capture sequencing evidence" RELATED [] +synonym: "circularized 3C sequencing" EXACT [] +synonym: "circularized 3C-seq" EXACT [] +is_a: ECO:0000232 ! chromosome conformation-based evidence +created_by: mchibucos +creation_date: 2010-11-30T03:22:25Z + +[Term] +id: ECO:0000240 +name: anatomical perturbation phenotypic evidence +def: "A type of experimental phenotypic evidence resulting from the disruption of a structural feature." [ECO:MCC] +synonym: "anatomical perturbation evidence" EXACT [] +is_a: ECO:0000059 ! experimental phenotypic evidence +created_by: mchibucos +creation_date: 2010-12-06T12:24:16Z + +[Term] +id: ECO:0000241 +name: environmental perturbation phenotypic evidence +def: "A type of experimental phenotypic evidence resulting from modification to the surroundings or conditions in which an organism lives or operates." [ECO:MCC] +comment: Many environmental factors are subject to modification, such as temperature, pressure, humidity, radiation, and so forth. +synonym: "environmental perturbation evidence" EXACT [] +synonym: "mechanical constraint evidence" RELATED [] +is_a: ECO:0000059 ! experimental phenotypic evidence +created_by: mchibucos +creation_date: 2010-12-06T12:33:58Z + +[Term] +id: ECO:0000242 +name: tissue ablation phenotypic evidence +def: "A type of anatomical perturbation phenotypic evidence resulting from the surgical removal of tissue or subcellular components." [ECO:MCC] +synonym: "ablated tissue evidence" EXACT [] +synonym: "tissue ablation evidence" EXACT [] +is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence +created_by: mchibucos +creation_date: 2010-12-06T12:33:58Z + +[Term] +id: ECO:0000243 +name: tissue grafting phenotypic evidence +def: "A type of anatomical perturbation phenotypic evidence resulting from the addition of tissue to an organism through a graft procedure." [ECO:MCC] +synonym: "tissue grafting evidence" EXACT [] +is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence +created_by: mchibucos +creation_date: 2010-12-06T12:33:58Z + +[Term] +id: ECO:0000244 +name: combinatorial evidence used in manual assertion +def: "A type of combinatorial analysis that is used in a manual assertion." [ECO:MCC] +comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments. +synonym: "inferred from in-silico analysis" RELATED [] +is_a: ECO:0000212 ! combinatorial evidence +is_a: ECO:0000352 ! evidence used in manual assertion +created_by: mchibucos +creation_date: 2010-12-09T02:02:12Z + +[Term] +id: ECO:0000245 +name: automatically integrated combinatorial evidence used in manual assertion +def: "A type of automatically integrated combinatorial evidence that is used in a manual assertion." [ECO:RCT] +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +synonym: "computational combinatorial evidence used in manual assertion" RELATED [] +synonym: "inferred from reviewed computational analysis" EXACT [GOECO:RCA] +synonym: "RCA" EXACT [GOECO:RCA] +xref: GOECO:RCA "inferred from reviewed computational analysis" +is_a: ECO:0000244 ! combinatorial evidence used in manual assertion +is_a: ECO:0007673 ! automatically integrated combinatorial evidence +property_value: ECO:9000002 "RCA" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/rca-inferred-reviewed-computational-analysis xsd:string +created_by: mchibucos +creation_date: 2010-12-09T02:37:54Z + +[Term] +id: ECO:0000246 +name: computational combinatorial evidence used in automatic assertion +def: "A type of computational combinatorial analysis that is used in an automatic assertion." [ECO:MCC] +comment: Merged with ECO:0000053. +is_obsolete: true +created_by: mchibucos +creation_date: 2010-12-09T02:37:54Z + +[Term] +id: ECO:0000247 +name: sequence alignment evidence used in manual assertion +def: "A type of sequence alignment evidence that is used in a manual assertion." [ECO:RCT] +subset: valid_with_gene +subset: valid_with_protein +synonym: "inferred from sequence alignment" EXACT [GOECO:ISA] +synonym: "ISA" EXACT [GOECO:ISA] +xref: GOECO:ISA "inferred from sequence alignment" +is_a: ECO:0000200 ! sequence alignment evidence +is_a: ECO:0000250 ! sequence similarity evidence used in manual assertion +property_value: ECO:9000002 "ISA" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/isa-inferred-sequence-alignment xsd:string +created_by: mchibucos +creation_date: 2010-12-09T05:12:27Z + +[Term] +id: ECO:0000248 +name: sequence alignment evidence used in automatic assertion +def: "A type of sequence alignment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000200 ! sequence alignment evidence +is_a: ECO:0000249 ! sequence similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-09T05:12:27Z + +[Term] +id: ECO:0000249 +name: sequence similarity evidence used in automatic assertion +def: "A type of sequence similarity evidence that is used in an automatic assertion." [ECO:RCT] +xref: GO_REF:0000107 "Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl" +is_a: ECO:0000044 ! sequence similarity evidence +is_a: ECO:0000251 ! similarity evidence used in automatic assertion +is_a: ECO:0007669 ! computational evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-09T05:22:30Z + +[Term] +id: ECO:0000250 +name: sequence similarity evidence used in manual assertion +def: "A type of sequence similarity evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +synonym: "inferred from sequence or structural similarity" EXACT [GOECO:ISS] +synonym: "ISS" EXACT [GOECO:ISS] +xref: GOECO:ISS "inferred from sequence or structural similarity" +is_a: ECO:0000044 ! sequence similarity evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +is_a: ECO:0007668 ! computational evidence used in manual assertion +property_value: ECO:9000002 "ISS" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/iss-inferred-sequence-or-structural-similarity xsd:string +created_by: mchibucos +creation_date: 2010-12-09T05:22:30Z + +[Term] +id: ECO:0000251 +name: similarity evidence used in automatic assertion +def: "A type of similarity evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "inferred from similarity" RELATED [] +synonym: "IS" BROAD [] +is_a: ECO:0000041 ! similarity evidence +is_a: ECO:0000501 ! evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-09T05:28:12Z + +[Term] +id: ECO:0000252 +name: similarity evidence used in manual assertion +def: "A type of similarity evidence that is used in a manual assertion." [ECO:RCT] +subset: go_groupings +synonym: "inferred from similarity" RELATED [] +synonym: "IS" BROAD [] +is_a: ECO:0000041 ! similarity evidence +is_a: ECO:0000352 ! evidence used in manual assertion +created_by: mchibucos +creation_date: 2010-12-09T05:28:12Z + +[Term] +id: ECO:0000253 +name: genetic similarity evidence used in manual assertion +def: "A type of genetic similarity evidence that is used in a manual assertion." [ECO:RCT] +synonym: "IGTS" BROAD [] +synonym: "inferred from genetic similarity" RELATED [] +is_a: ECO:0000051 ! genetic similarity evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +created_by: mchibucos +creation_date: 2010-12-10T01:42:44Z + +[Term] +id: ECO:0000254 +name: genetic similarity evidence used in automatic assertion +def: "A type of genetic similarity evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "IGTS" BROAD [] +synonym: "inferred from genetic similarity" RELATED [] +is_a: ECO:0000051 ! genetic similarity evidence +is_a: ECO:0000251 ! similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T01:42:44Z + +[Term] +id: ECO:0000255 +name: match to sequence model evidence used in manual assertion +def: "A type of match to sequence model evidence that is used in a manual assertion." [ECO:RCT] +synonym: "inferred from sequence model" EXACT [GOECO:ISM] +synonym: "ISM" EXACT [GOECO:ISM] +xref: GO_REF:0000011 "Hidden Markov Models (TIGR)" +xref: GOECO:ISM "inferred from sequence model" +is_a: ECO:0000202 ! match to sequence model evidence +is_a: ECO:0000250 ! sequence similarity evidence used in manual assertion +property_value: ECO:9000002 "ISM" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ism-inferred-sequence-model xsd:string +created_by: mchibucos +creation_date: 2010-12-10T02:26:35Z + +[Term] +id: ECO:0000256 +name: match to sequence model evidence used in automatic assertion +def: "A type of match to sequence model evidence that is used in an automatic assertion." [ECO:RCT] +xref: GO_REF:0000002 "Gene Ontology annotation through association of InterPro records with GO terms." +is_a: ECO:0000202 ! match to sequence model evidence +is_a: ECO:0000249 ! sequence similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T02:26:35Z + +[Term] +id: ECO:0000257 +name: motif similarity evidence used in manual assertion +def: "A type of motif similarity evidence that is used in a manual assertion." [ECO:RCT] +synonym: "recognized domains" RELATED [] +is_a: ECO:0000028 ! motif similarity evidence +is_a: ECO:0000255 ! match to sequence model evidence used in manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T02:44:34Z + +[Term] +id: ECO:0000258 +name: motif similarity evidence used in automatic assertion +def: "A type of motif similarity evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "recognized domains" RELATED [] +is_a: ECO:0000028 ! motif similarity evidence +is_a: ECO:0000256 ! match to sequence model evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T02:44:34Z + +[Term] +id: ECO:0000259 +name: match to InterPro member signature evidence used in automatic assertion +def: "A type of match to InterPro member signature evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000029 ! match to InterPro member signature evidence +is_a: ECO:0000256 ! match to sequence model evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T02:54:51Z + +[Term] +id: ECO:0000260 +name: match to InterPro member signature evidence used in manual assertion +def: "A type of match to InterPro member signature evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000029 ! match to InterPro member signature evidence +is_a: ECO:0000255 ! match to sequence model evidence used in manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T02:54:51Z + +[Term] +id: ECO:0000261 +name: targeting sequence prediction evidence used in automatic assertion +def: "A type of targeting sequence prediction that is used in an automatic assertion." [ECO:RCT] +synonym: "targeting sequence prediction" RELATED [] +is_a: ECO:0000081 ! targeting sequence prediction evidence +is_a: ECO:0000258 ! motif similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T03:02:49Z + +[Term] +id: ECO:0000262 +name: targeting sequence prediction evidence used in manual assertion +def: "A type of targeting sequence prediction that is used in a manual assertion." [ECO:RCT] +synonym: "targeting sequence prediction" RELATED [] +is_a: ECO:0000081 ! targeting sequence prediction evidence +is_a: ECO:0000257 ! motif similarity evidence used in manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T03:02:49Z + +[Term] +id: ECO:0000263 +name: transmembrane domain prediction evidence used in automatic assertion +def: "A type of transmembrane domain prediction that is used in an automatic assertion." [ECO:RCT] +synonym: "transmembrane domain prediction" RELATED [] +is_a: ECO:0000083 ! transmembrane domain prediction evidence +is_a: ECO:0000258 ! motif similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T03:28:35Z + +[Term] +id: ECO:0000264 +name: transmembrane domain prediction evidence used in manual assertion +def: "A type of transmembrane domain prediction that is used in a manual assertion." [ECO:RCT] +synonym: "transmembrane domain prediction" RELATED [] +is_a: ECO:0000083 ! transmembrane domain prediction evidence +is_a: ECO:0000257 ! motif similarity evidence used in manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T03:28:35Z + +[Term] +id: ECO:0000265 +name: sequence orthology evidence used in automatic assertion +def: "A type of sequence orthology evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "Ortholog evidence" RELATED [] +synonym: "ortholog evidence" RELATED [] +xref: GO_REF:0000035 "Automatic transfer of experimentally verified manual GO annotation data to plant orthologs using Ensembl Compara" +is_a: ECO:0000201 ! sequence orthology evidence +is_a: ECO:0000249 ! sequence similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T03:39:41Z + +[Term] +id: ECO:0000266 +name: sequence orthology evidence used in manual assertion +def: "A type of sequence orthology evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +synonym: "inferred from sequence orthology" EXACT [] {name="GOECO:ISO"} +synonym: "ISO" EXACT [GOECO:ISO] +synonym: "Ortholog evidence" RELATED [] +synonym: "ortholog evidence" RELATED [] +xref: GOECO:ISO "inferred from sequence orthology" +is_a: ECO:0000201 ! sequence orthology evidence +is_a: ECO:0000250 ! sequence similarity evidence used in manual assertion +property_value: ECO:9000002 "ISO" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/iso-inferred-sequence-orthology xsd:string +created_by: mchibucos +creation_date: 2010-12-10T03:39:41Z + +[Term] +id: ECO:0000267 +name: enzyme-linked immunoabsorbent assay evidence +def: "A type of protein detection assay evidence where an antigen is bound to a substrate, and an antibody directly or indirectly linked to an enzyme is utilized to determine the amount of bound antigen which is visualized with a color change in the substrate." [ECO:MCC, ECO:SW, PMID:23949770] +comment: ELISA is sometimes used in the study of transcriptional regulation to identify a produced protein, especially secreted protein. +synonym: "ELISA evidence" EXACT [] +synonym: "enzyme-linked immunosorbent assay" NARROW [] +xref: MI:0411 "enzyme linked immunosorbent assay" +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: mchibucos +creation_date: 2010-12-14T01:57:13Z + +[Term] +id: ECO:0000268 +name: flow cytometry evidence +def: "A type of direct assay evidence in which cells or particles are characterized by passing them through a light source and monitoring the resultant light scatter or fluorochrome excitation." [ECO:MCC] +synonym: "FCM" EXACT [] +is_a: ECO:0000002 ! direct assay evidence +created_by: mchibucos +creation_date: 2010-12-14T02:07:08Z + +[Term] +id: ECO:0000269 +name: experimental evidence used in manual assertion +def: "A type of experimental evidence that is used in a manual assertion." [ECO:MCC] +synonym: "EXP" EXACT [GOECO:EXP] +synonym: "inferred from experiment" EXACT [GOECO:EXP] +xref: GOECO:EXP "inferred from experiment" +is_a: ECO:0000006 ! experimental evidence +is_a: ECO:0000352 ! evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/exp-inferred-experiment xsd:string +created_by: mchibucos +creation_date: 2010-12-20T05:36:49Z + +[Term] +id: ECO:0000270 +name: expression pattern evidence used in manual assertion +def: "A type of expression pattern evidence that is used in a manual assertion." [ECO:MCC] +synonym: "IEP" EXACT [GOECO:IEP] +synonym: "inferred from expression pattern" EXACT [GOECO:IEP] +xref: GOECO:IEP "inferred from expression pattern" +is_a: ECO:0000008 ! expression pattern evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/iep-inferred-expression-pattern xsd:string +created_by: mchibucos +creation_date: 2010-12-20T05:46:39Z + +[Term] +id: ECO:0000271 +name: array experiment evidence used in manual assertion +comment: This general 'array experiment' term should not be used - replace with the specific type of array (e.g. ECO:0000273, ECO:0000276, ECO:0000285, etc.) +is_obsolete: true +created_by: mchibucos +creation_date: 2010-12-20T05:51:58Z + +[Term] +id: ECO:0000272 +name: Affymetrix GeneChip evidence used in manual assertion +def: "A type of Affymetrix GeneChip evidence that is used in a manual assertion." [ECO:MCC] +synonym: "Affymetrix array experiment evidence" BROAD [] +is_a: ECO:0000101 ! Affymetrix GeneChip evidence +is_a: ECO:0000276 ! cRNA to DNA expression microarray evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T05:53:36Z + +[Term] +id: ECO:0000273 +name: cDNA to DNA expression microarray evidence used in manual assertion +alt_id: ECO:0001178 +alt_id: ECO:0005525 +def: "A type of cDNA to DNA expression microarray evidence that is used in a manual assertion." [ECO:MCC] +synonym: "DNA microarray|RNA microarray" RELATED [] +is_a: ECO:0000097 ! cDNA to DNA expression microarray evidence +is_a: ECO:0000275 ! expression microarray evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T05:55:49Z + +[Term] +id: ECO:0000274 +name: differential methylation hybridization evidence used in manual assertion +def: "A type of differential methylation hybridization evidence that is used in a manual assertion." [ECO:RCT] +synonym: "CpG island microarray evidence" BROAD [] +synonym: "CpG island microarray evidence used in manual assertion" EXACT [] +is_a: ECO:0000069 ! differential methylation hybridization evidence +is_a: ECO:0001198 ! in vitro methylation assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T05:58:44Z + +[Term] +id: ECO:0000275 +name: expression microarray evidence used in manual assertion +def: "A type of expression microarray evidence that is used in a manual assertion." [ECO:MCC] +synonym: "differential gene expression evidence from microarray experiment" RELATED [] +is_a: ECO:0000058 ! expression microarray evidence +is_a: ECO:0000291 ! transcript expression evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:01:26Z + +[Term] +id: ECO:0000276 +name: cRNA to DNA expression microarray evidence used in manual assertion +def: "A type of cRNA to DNA expression microarray evidence that is used in a manual assertion." [ECO:RCT] +synonym: "genomic microarray evidence" BROAD [] +synonym: "genomic microarray evidence used in manual assertion" EXACT [] +is_a: ECO:0000062 ! cRNA to DNA expression microarray evidence +is_a: ECO:0000275 ! expression microarray evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:03:28Z + +[Term] +id: ECO:0000277 +name: Nimblegen array evidence used in manual assertion +def: "A type of evidence arising from a Nimblegen array experiment that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000105 ! Nimblegen array evidence +is_a: ECO:0000273 ! cDNA to DNA expression microarray evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:05:20Z + +[Term] +id: ECO:0000278 +name: array-based sequence capture evidence used in manual assertion +def: "A type of array-based sequence capture evidence that is used in a manual assertion." [ECO:MCC] +synonym: "DNA microarray" BROAD [] +synonym: "oligonucleotide microarray evidence" BROAD [] +synonym: "oligonucleotide microarray evidence used in manual assertion" BROAD [] +synonym: "single nucleotide polymorphism array evidence" RELATED [] +synonym: "SNP array evidence" RELATED [] +is_a: ECO:0000093 ! array-based sequence capture evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:09:45Z + +[Term] +id: ECO:0000279 +name: qualitative western immunoblotting evidence used in manual assertion +alt_id: ECO:0007183 +def: "A type of qualitative western immunoblotting evidence that is used in a manual assertion." [ECO:MCC] +synonym: "protein expression level evidence based on western blot used in manual assertion" EXACT [] +synonym: "protein immunoblot" RELATED [] +synonym: "protein levels (e.g. Western blots)" RELATED [] +synonym: "Western blot expression analysis" RELATED [] +is_a: ECO:0000112 ! qualitative western immunoblotting evidence +is_a: ECO:0000284 ! protein expression evidence used in manual assertion +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:20:01Z + +[Term] +id: ECO:0000281 +name: expression library screen evidence used in manual assertion +def: "A type of expression library screen evidence that is used in a manual assertion." [ECO:MCC] +synonym: "expression library screening" RELATED [] +is_a: ECO:0000114 ! expression library screen evidence +is_a: ECO:0000284 ! protein expression evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:24:05Z + +[Term] +id: ECO:0000282 +name: heterologous protein expression evidence used in manual assertion +def: "A type of heterologous protein expression analysis that is used in a manual assertion." [ECO:MCC] +synonym: "protein expression in heterologous system" RELATED [] +is_a: ECO:0000154 ! heterologous protein expression evidence +is_a: ECO:0000284 ! protein expression evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:27:04Z + +[Term] +id: ECO:0000283 +name: spatial pattern of protein expression evidence used in manual assertion +def: "A type of protein expression spatial pattern analysis that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000045 ! spatial pattern of protein expression evidence +is_a: ECO:0000284 ! protein expression evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:31:44Z + +[Term] +id: ECO:0000284 +name: protein expression evidence used in manual assertion +alt_id: ECO:0000280 +def: "A type of protein expression analysis that is used in a manual assertion." [ECO:MCC] +synonym: "protein expression level evidence used in manual assertion" EXACT [] +is_a: ECO:0000010 ! protein expression evidence +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:33:46Z + +[Term] +id: ECO:0000285 +name: DNA to cDNA expression microarray evidence used in manual assertion +def: "A type of DNA to cDNA expression microarray evidence that is used in a manual assertion." [ECO:MCC] +synonym: "microarray RNA expression level evidence" BROAD [] +synonym: "REM evidence" BROAD [] +synonym: "RNA expression microarray evidence" BROAD [] +synonym: "transcript levels (e.g. microarray data)" RELATED [] +is_a: ECO:0000104 ! DNA to cDNA expression microarray evidence +is_a: ECO:0000275 ! expression microarray evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:41:33Z + +[Term] +id: ECO:0000287 +name: differential hybridization evidence used in manual assertion +def: "A type of differential hybridization experiment that is used in a manual assertion." [ECO:MCC] +synonym: "differential hybridization" RELATED [] +is_a: ECO:0000116 ! differential hybridization evidence +is_a: ECO:0000291 ! transcript expression evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:55:14Z + +[Term] +id: ECO:0000288 +name: RNA protection assay evidence used in manual assertion +def: "A type of RNA protection assay that is used in a manual assertion." [ECO:MCC] +synonym: "ribonuclease protection assay" BROAD [] +synonym: "RNA protection assay|RPA" RELATED [] +synonym: "RNAse protection assay" RELATED [] +is_a: ECO:0000110 ! RNA protection assay evidence +is_a: ECO:0001240 ! nuclease protection assay evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:57:33Z + +[Term] +id: ECO:0000289 +name: spatial pattern of transcript expression evidence used in manual assertion +def: "A type of spatial pattern of transcript expression analysis that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000047 ! spatial pattern of transcript expression evidence +is_a: ECO:0000291 ! transcript expression evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:59:18Z + +[Term] +id: ECO:0000290 +name: subtractive hybridization evidence used in manual assertion +def: "A type of subtractive hybridization experiment that is used in a manual assertion." [ECO:MCC] +synonym: "subtractive hybridization" RELATED [] +is_a: ECO:0000118 ! subtractive hybridization evidence +is_a: ECO:0000291 ! transcript expression evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T07:00:48Z + +[Term] +id: ECO:0000291 +name: transcript expression evidence used in manual assertion +alt_id: ECO:0000286 +def: "A type of transcript expression evidence that is used in a manual assertion." [ECO:MCC] +synonym: "transcript expression level evidence" BROAD [] +synonym: "transcriptomics evidence used in manual assertion" EXACT [] +is_a: ECO:0000009 ! transcript expression evidence +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T07:02:08Z + +[Term] +id: ECO:0000292 +name: morpholino experiment evidence +def: "A type of mutant phenotype evidence that uses anti-sense by introducing morpholino oligonucleotides into the cytosol of a cell." [ECO:MCC] +comment: Morpholino oligonucleotides modify gene expression by blocking access of other molecules to specific, approximately 25-base-long regions of the base-pairing surfaces of ribonucleic acid (RNA). To achieve this anti-sense knockdown technique, delivery of the morpholino can be achieved via microinjection, electroporation, or another method. +synonym: "anti-sense" BROAD [] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: mchibucos +creation_date: 2011-01-10T03:36:25Z + +[Term] +id: ECO:0000293 +name: systematic evolution of ligands by exponential amplification evidence +def: "A type of evidence arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand." [ECO:MCC, url:http\://en.wikipedia.org/wiki/Systematic_Evolution_of_Ligands_by_Exponential_Enrichment] +comment: SELEX begins with the synthesis of a large oligonucleotide library, either single-stranded DNA or RNA, which consists of random fixed-length sequences flanked by constant 5' and 3' ends that serve as primers. The library is exposed to a target ligand, typically a protein or small organic compound. Unbound sequences are removed, typically with affinity chromatography. Bound sequences are eluted and the nucleic acid is extracted, followed by PCR amplification (RNA is first reverse transcribed). PCR product is converted to single stranded (DNA) or transcribed (RNA). Oligonucleotides are bound to the ligand again and the process is repeated with increasing elution stringency. After several rounds of the process, recovered oligonucleotides are sequenced and analyzed to reveal the binding specificity of the protein. +synonym: "in vitro evolution evidence" RELATED [] +synonym: "in vitro selection evidence" EXACT [] +synonym: "SELEX evidence" EXACT [] +xref: MI:0657 "systematic evolution of ligands by exponential enrichment" +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000112 "A total of 160 SELEX fragments were cloned and sequenced, which were classified into 29 CRP-binding sequences, including 13 previously identified loci and 16 newly identified sites (Table S2). When the CRP-binding sequences are located upstream or near promoters of the flanking genes, these genes are predicted to be under the control of CRP." xsd:string {xref="PMID:21673794"} +created_by: mchibucos +creation_date: 2011-01-10T04:23:50Z + +[Term] +id: ECO:0000294 +name: bacterial one-hybrid evidence +def: "A type of bait-prey hybrid interaction evidence that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey)." [ECO:MCC] +comment: Bacterial one-hybrid assay is a method for screening a library of candidate proteins (prey plasmid) for the ability to bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to reporter genes (bait plasmid). Transformation of a bacterial host with two different plasmids is required: One is designed to express the "bait". The other plasmid contains a "prey" which, if bound to by the chimeric fusion product, drives expression of downstream reporter genes. +synonym: "B1H evidence" EXACT [] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence +property_value: IAO:0000112 "Bacterial one-hybrid assays confirmed the interaction between MtrA and the regulatory sequence of the dnaA initiator gene." xsd:string {xref="PMID:20843371"} +created_by: mchibucos +creation_date: 2011-01-10T06:16:20Z + +[Term] +id: ECO:0000295 +name: RNA-sequencing evidence +alt_id: ECO:0000357 +def: "A type of transcript evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC] +comment: Total RNA is isolated from cells and mRNA is purified by targeting the 3' polyadenylated (poly(A)) tail with poly(T) oligos covalently attached to a substrate. Next, cDNA fragments are generated either by hydrolysis of RNA into 200-300 base oligonucleotides, preceded by reverse transcription, or by reverse transcription initiated by random primers. The cDNA second strand is synthesized, fragments are adapter ligated and high-throughput sequencing is performed by Roche 454, Illumina, ABI-SOLiD, or another HT sequencing technology. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed. +synonym: "differential gene expression evidence from RNA-seq experiment" NARROW [] +synonym: "RNA sequencing" RELATED [] +synonym: "RNA-seq evidence" EXACT [] +synonym: "RNAseq evidence" EXACT [] +synonym: "whole transcriptome shotgun sequencing" EXACT [PMID:18611170] +synonym: "WTSS" EXACT [] +is_a: ECO:0000009 ! transcript expression evidence +created_by: mchibucos +creation_date: 2011-01-11T12:54:50Z + +[Term] +id: ECO:0000298 +name: cleavage arrested development evidence +def: "A type of experimental phenotypic evidence where embryonic development proceeds although cytokinesis is blocked." [ECO:MCC] +comment: In the embryos of some organisms, inhibition of cytokinesis blocks neither the cell cycle nor the unfolding of the developmental program. As such, embryonic morphology can be frozen, allowing analysis of the developmental fate of each cell present at the time of cleavage arrest. +is_a: ECO:0000059 ! experimental phenotypic evidence +created_by: mchibucos +creation_date: 2011-01-11T03:28:49Z + +[Term] +id: ECO:0000299 +name: cytochalasin experiment evidence +def: "A type of cleavage arrested development that arises after treatment with cytochalasin." [ECO:MCC] +comment: Cytochalasin inhibits cytokinesis by blocking the formation of contractile microfilaments. +is_a: ECO:0000298 ! cleavage arrested development evidence +created_by: mchibucos +creation_date: 2011-01-11T03:30:29Z + +[Term] +id: ECO:0000300 +name: green fluorescent protein immunolocalization evidence +def: "A type of immunolocalization evidence data that was generated using green fluorescent protein (GFP) as a marker." [ECO:MCC] +comment: This term describes immunolocalization of the protein; to describe transcriptional activation, use the term "localization of green fluorescent protein transcript". +synonym: "GFP immunolocalization evidence" EXACT [] +is_a: ECO:0000087 ! immunolocalization evidence +created_by: mchibucos +creation_date: 2011-01-11T03:48:50Z + +[Term] +id: ECO:0000301 +name: beta-galactosidase protein immunolocalization evidence +def: "A type of immunolocalization evidence that was generated using LacZ protein as a marker." [ECO:MCC] +comment: This term describes immunolocalization of the protein; to describe transcriptional activation, use the term "localization of LacZ transcript". +synonym: "LacZ protein immunolocalization evidence" EXACT [] +is_a: ECO:0000087 ! immunolocalization evidence +created_by: mchibucos +creation_date: 2011-01-11T04:06:40Z + +[Term] +id: ECO:0000302 +name: author statement used in manual assertion +alt_id: ECO:0007764 +def: "A type of author statement that is used in a manual assertion." [ECO:MCC] +synonym: "author inference used in manual assertion" EXACT [] +is_a: ECO:0000204 ! author statement +is_a: ECO:0006155 ! documented statement evidence used in manual assertion +created_by: mchibucos +creation_date: 2011-01-20T02:26:47Z + +[Term] +id: ECO:0000303 +name: author statement without traceable support used in manual assertion +def: "A type of author statement without traceable support that is used in a manual assertion." [ECO:MCC] +synonym: "NAS" EXACT [GOECO:NAS] +synonym: "non-traceable author statement" RELATED [GOECO:NAS] +xref: GOECO:NAS "non-traceable author statement" +is_a: ECO:0000034 ! author statement without traceable support +is_a: ECO:0000302 ! author statement used in manual assertion +property_value: ECO:9000002 "NAS" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/nas-non-traceable-author-statement xsd:string +created_by: mchibucos +creation_date: 2011-01-20T02:29:50Z + +[Term] +id: ECO:0000304 +name: author statement supported by traceable reference used in manual assertion +def: "A type of author statement supported by traceable reference that is used in a manual assertion." [ECO:MCC] +synonym: "TAS" EXACT [GOECO:TAS] +synonym: "traceable author statement" RELATED [GOECO:TAS] +xref: GOECO:TAS "traceable author statement" +is_a: ECO:0000033 ! author statement supported by traceable reference +is_a: ECO:0000302 ! author statement used in manual assertion +property_value: ECO:9000002 "TAS" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/tas-traceable-author-statement xsd:string +created_by: mchibucos +creation_date: 2011-01-20T02:31:03Z + +[Term] +id: ECO:0000305 +name: curator inference used in manual assertion +def: "A type of curator inference that is used in a manual assertion." [ECO:MCC] +subset: valid_with_biological_process +subset: valid_with_cellular_component +subset: valid_with_molecular_function +synonym: "IC" EXACT [GOECO:IC] +synonym: "inferred by curator" EXACT [GOECO:IC] +xref: GOECO:IC "inferred by curator" +is_a: ECO:0000205 ! curator inference +is_a: ECO:0007751 ! inferential evidence used in manual assertion +property_value: ECO:9000002 "IC" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ic-inferred-curator xsd:string +created_by: mchibucos +creation_date: 2011-01-20T02:33:32Z + +[Term] +id: ECO:0000306 +name: inference from background scientific knowledge used in manual assertion +def: "A type of inference from background scientific knowledge that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000001 ! inference from background scientific knowledge +is_a: ECO:0000305 ! curator inference used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: mchibucos +creation_date: 2011-01-20T02:35:11Z + +[Term] +id: ECO:0000307 +name: no evidence data found used in manual assertion +def: "An inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources that is used in an manual assertion." [ECO:MCC] +synonym: "ND" EXACT [GOECO:ND] +synonym: "no biological data available" EXACT [GOECO:ND] +xref: GOECO:ND "no biological data available" +is_a: ECO:0000035 ! no evidence data found +is_a: ECO:0000305 ! curator inference used in manual assertion +property_value: ECO:9000002 "ND" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/nd-no-biological-data-available xsd:string +created_by: mchibucos +creation_date: 2011-01-20T02:43:18Z + +[Term] +id: ECO:0000308 +name: biological aspect of ancestor evidence +def: "A type of phylogenetic evidence whereby an aspect of a descendant gene is inferred through the characterization of an aspect of an ancestral gene." [ECO:MCC] +comment: First, a characteristic of the most recent common ancestor (MRCA) is inferred from experimental evidence in descendants; this is followed by inferring a characteristic of a descendant of the MRCA. This type of evidence can be used in support of both positive and "not" GO annotations. +is_a: ECO:0000080 ! phylogenetic evidence +created_by: mchibucos +creation_date: 2011-02-04T05:41:26Z + +[Term] +id: ECO:0000309 +name: cap analysis of gene expression evidence +def: "A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by a cap-trapper system." [ECO:MCC] +comment: Complementary DNA (cDNA) is synthesized from microgram quantities of mRNA using first-strand cDNA primer (oligo dT12-18) and reverse transcriptase in the presence of trehalose and sorbitol, followed by selection of full-length cDNA with biotinylated cap-trapper. Linkers are attached to the 5' ends of full-length enriched cDNAs to introduce a recognition site for the restriction endonuclease MmeI, which creates a two base overhang. After amplification, sequencing tags are concatenated for high-throughput sequencing. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed. +synonym: "CAGE" EXACT [PMID:16489339] +is_a: ECO:0000009 ! transcript expression evidence +created_by: mchibucos +creation_date: 2011-02-17T01:33:09Z + +[Term] +id: ECO:0000310 +name: nano-cap analysis of gene expression evidence +def: "A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by reverse transcriptase template switching." [ECO:MCC] +comment: NanoCAGE captures the 5' ends of molecules by template switching. When polymerizing the cDNA of a capped mRNA, the reverse transcriptase adds extra cytosines that are complementary to the cap. Each 5' full length cDNAs is extended upon hybridization of the riboguanosine-tailed template switching oligonucleotides to these extra cytosines. In semisuppressive PCR, the short templates fold intramolecularly and prevent the binding of primers, which precludes amplification; longer molecules are less likely to fold and are thus amplified. Templates derived from reaction artifacts form stable homoduplexes, also precluding amplification. After template switching, semisuppressive PCR, and EcoP151 cleavage, 25-bp tags are ligated to bar code-containing oligonucleotide adaptors. After PCR amplification, the nanoCAGE tags are sequenced by synthesis. NanoCAGE requires as little as 10 nanograms of total RNA. +synonym: "nanoCAGE" RELATED [PMID:20543846] +is_a: ECO:0000009 ! transcript expression evidence +created_by: mchibucos +creation_date: 2011-02-17T01:33:34Z + +[Term] +id: ECO:0000311 +name: imported information +def: "A type of documented statement evidence that is based on work performed by a person or group prior to a use by a different person or group." [ECO:MCC] +is_a: ECO:0006151 ! documented statement evidence +created_by: mchibucos +creation_date: 2011-03-02T05:24:13Z + +[Term] +id: ECO:0000312 +name: imported information used in manual assertion +def: "A type of imported information that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000311 ! imported information +is_a: ECO:0006155 ! documented statement evidence used in manual assertion +created_by: mchibucos +creation_date: 2011-03-02T05:29:44Z + +[Term] +id: ECO:0000313 +name: imported information used in automatic assertion +def: "A type of imported information that is used in an automatic assertion." [ECO:MCC] +is_a: ECO:0000311 ! imported information +is_a: ECO:0006156 ! documented statement evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2011-03-02T05:33:43Z + +[Term] +id: ECO:0000314 +name: direct assay evidence used in manual assertion +def: "A type of direct assay evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_chemical_entity +synonym: "IDA" EXACT [GOECO:IDA] +synonym: "inferred from direct assay" EXACT [GOECO:IDA] +xref: GOECO:IDA "inferred from direct assay" +is_a: ECO:0000002 ! direct assay evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ida-inferred-direct-assay xsd:string +created_by: mchibucos +creation_date: 2011-10-28T04:59:31Z + +[Term] +id: ECO:0000315 +name: mutant phenotype evidence used in manual assertion +def: "A type of mutant phenotype evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_chemical_entity +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +synonym: "IMP" EXACT [GOECO:IMP] +synonym: "inferred from mutant phenotype" EXACT [GOECO:IMP] +xref: GOECO:IMP "inferred from mutant phenotype" +is_a: ECO:0000015 ! mutant phenotype evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/imp-inferred-mutant-phenotype xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:12:49Z + +[Term] +id: ECO:0000316 +name: genetic interaction evidence used in manual assertion +def: "A type of genetic interaction experiment evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +synonym: "IGI" EXACT [GOECO:IGI] +synonym: "inferred from genetic interaction" EXACT [GOECO:IGI] +xref: GOECO:IGI "inferred from genetic interaction" +is_a: ECO:0000011 ! genetic interaction evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "IGI" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/igi-inferred-genetic-interaction xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:14:42Z + +[Term] +id: ECO:0000317 +name: genomic context evidence used in manual assertion +def: "A type of genomic context evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +synonym: "IGC" EXACT [GOECO:IGC] +synonym: "inferred from genomic context" EXACT [GOECO:IGC] +xref: GOECO:IGC "inferred from genomic context" +is_a: ECO:0000177 ! genomic context evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +property_value: ECO:9000002 "IGC" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/igc-inferred-genomic-context xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:22:53Z + +[Term] +id: ECO:0000318 +name: biological aspect of ancestor evidence used in manual assertion +def: "A type of biological aspect of ancestor evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +synonym: "IBA" EXACT [GOECO:IBA] +synonym: "inferred from biological aspect of ancestor" EXACT [GOECO:IBA] +xref: GOECO:IBA "inferred from biological aspect of ancestor" +is_a: ECO:0000252 ! similarity evidence used in manual assertion +is_a: ECO:0000308 ! biological aspect of ancestor evidence +property_value: ECO:9000002 "IBA" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/iba-inferred-biological-aspect-ancestor xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:23:51Z + +[Term] +id: ECO:0000319 +name: biological aspect of descendant evidence used in manual assertion +def: "A type of biological aspect of descendant evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +synonym: "IBD" EXACT [GOECO:IBD] +synonym: "inferred from biological aspect of descendant" EXACT [GOECO:IBD] +xref: GOECO:IBD "inferred from biological aspect of descendant" +is_a: ECO:0000214 ! biological aspect of descendant evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +property_value: ECO:9000002 "IBD" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ibd-inferred-biological-aspect-descendent xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:25:09Z + +[Term] +id: ECO:0000320 +name: phylogenetic determination of loss of key residues evidence used in manual assertion +def: "A type of phylogenetic determination of loss of key residues evidence that is used in a manual assertion." [ECO:MCC] +synonym: "IKR" EXACT [GOECO:IKR] +synonym: "IMR" EXACT [GOECO:IKR] +synonym: "inferred from key residues" EXACT [GOECO:IKR] +synonym: "inferred from missing residues" EXACT [GOECO:IKR] +xref: GOECO:IKR "inferred from key residues" +xref: GOECO:IMR "inferred from missing residues" +is_a: ECO:0000216 ! phylogenetic determination of loss of key residues evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +property_value: ECO:9000002 "IKR" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ikr-inferred-key-residues xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:25:48Z + +[Term] +id: ECO:0000321 +name: rapid divergence from ancestral sequence evidence used in manual assertion +def: "A type of rapid divergence from ancestral sequence evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +synonym: "inferred from rapid divergence" EXACT [GOECO:IRD] +synonym: "IRD" EXACT [GOECO:IRD] +xref: GOECO:IRD "inferred from rapid divergence" +is_a: ECO:0000215 ! rapid divergence from ancestral sequence evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +property_value: ECO:9000002 "IRD" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ird-inferred-rapid-divergence xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:26:42Z + +[Term] +id: ECO:0000322 +name: imported manually asserted information used in automatic assertion +def: "Evidence that was initially used in manual assertion by one person or group, which is imported by a second person or group and used in automatic assertion." [ECO:MCC] +is_a: ECO:0000313 ! imported information used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2011-12-13T12:04:48Z + +[Term] +id: ECO:0000323 +name: imported automatically asserted information used in automatic assertion +def: "A type of imported information used in automatic assertion that was initially used in automatic assertion by one person or group, which is imported by a second person or group and used in automatic assertion." [ECO:MCC] +is_a: ECO:0000313 ! imported information used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2011-12-13T02:06:25Z + +[Term] +id: ECO:0000324 +name: imaging assay evidence +alt_id: ECO:0001122 +alt_id: ECO:0005024 +def: "A type of direct assay evidence in which an image is created and analyzed." [] +synonym: "radiologic test evidence" NARROW [] +xref: ECO:MCC +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000112 "In the images shown in Figure 9, the MelR focus is 15 x 33 nm across the shortest and longest axes of the oval in the complex with the shortened arm (right hand panel), compared to 9 x 12 nm in the other complex (centre panel). The simplest explanation for these observations, that is consistent with the location of sites in the DNA fragment (Figure 9), is that one type of complex contains 3 MelR molecules bound at sites 2, 1 and 1' (centre panel), whilst the other contains 4 MelR molecules bound at sites 2, 1, 1' and R (right panel)." xsd:string {xref="PMID:18346968"} +created_by: mchibucos +creation_date: 2012-02-23T12:01:43Z + +[Term] +id: ECO:0000325 +name: chromatography evidence +def: "A type of experimental evidence that is based on separation of constituent parts of a mixture (the mobile phase) as they pass differentially through a stationary phase due to differences in partition coefficient and retention on the stationary phase." [ECO:MCC] +synonym: "chromatographic evidence" EXACT [] +is_a: ECO:0000006 ! experimental evidence +created_by: mchibucos +creation_date: 2012-05-16T11:46:27Z + +[Term] +id: ECO:0000326 +name: transcript splice pattern evidence +def: "A type of sequence alignment evidence that is based on comparing the position of exon junctions, relative to a reference genome, for a transcript annotation and a supporting transcript." [ECO:MCC] +is_a: ECO:0000200 ! sequence alignment evidence +created_by: mchibucos +creation_date: 2012-06-08T11:03:59Z + +[Term] +id: ECO:0000327 +name: whole transcript splice pattern evidence +def: "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features." [ECO:MCC] +comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child. +is_a: ECO:0000326 ! transcript splice pattern evidence +created_by: mchibucos +creation_date: 2012-06-08T11:11:24Z + +[Term] +id: ECO:0000328 +name: coding sequence splice pattern evidence +def: "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript." [ECO:MCC] +comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child. +is_a: ECO:0000326 ! transcript splice pattern evidence +created_by: mchibucos +creation_date: 2012-06-08T11:21:57Z + +[Term] +id: ECO:0000329 +name: whole transcript splice pattern evidence used in manual assertion +def: "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in manual assertion." [ECO:MCC] +comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child. +is_a: ECO:0000247 ! sequence alignment evidence used in manual assertion +is_a: ECO:0000327 ! whole transcript splice pattern evidence +property_value: ECO:9000002 "ISA" xsd:string +created_by: mchibucos +creation_date: 2012-06-08T11:40:03Z + +[Term] +id: ECO:0000330 +name: coding sequence splice pattern evidence used in manual assertion +def: "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in manual assertion." [ECO:MCC] +comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child. +is_a: ECO:0000247 ! sequence alignment evidence used in manual assertion +is_a: ECO:0000328 ! coding sequence splice pattern evidence +property_value: ECO:9000002 "ISA" xsd:string +created_by: mchibucos +creation_date: 2012-06-08T11:43:47Z + +[Term] +id: ECO:0000331 +name: coding sequence splice pattern evidence used in automatic assertion +def: "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in automatic assertion." [ECO:MCC] +comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child. +is_a: ECO:0000328 ! coding sequence splice pattern evidence +is_a: ECO:0007825 ! transcript splice pattern evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2012-06-08T11:48:09Z + +[Term] +id: ECO:0000332 +name: whole transcript splice pattern evidence used in automatic assertion +def: "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in automatic assertion." [ECO:MCC] +comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child. +is_a: ECO:0000327 ! whole transcript splice pattern evidence +is_a: ECO:0007825 ! transcript splice pattern evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2012-06-08T11:49:17Z + +[Term] +id: ECO:0000333 +name: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence +def: "A type of gel electrophoresis evidence where proteins are separated according to their electrophoretic mobility." [ECO:MCC, PubMed:4861258, url:http\://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html, url:http\://www.ncbi.nlm.nih.gov/Class/NAWBIS/Modules/Protein/protein18.html] +comment: Electrophoretic mobility is a function of both the length and charge of a protein. The protein sample is linearized with sodium dodecyl sulfate (SDS), an anionic detergent. Typically, polypeptides bound with SDS possess an even distribution of charge per unit mass, and subsequent fractionation is by size. +synonym: "SDS-PAGE evidence" EXACT [] +is_a: ECO:0000337 ! gel electrophoresis evidence +created_by: mchibucos +creation_date: 2012-07-31T02:56:43Z + +[Term] +id: ECO:0000334 +name: particle size and count assay evidence +def: "A type of direct assay evidence resulting from an assay where both the size of and number of particles in a sample are determined." [ECO:MCC] +comment: An example of an instrument used to analyze particle sizes and counts is the Beckman Coulter® Z Series system. +is_a: ECO:0000002 ! direct assay evidence +created_by: mchibucos +creation_date: 2012-07-31T03:19:32Z + +[Term] +id: ECO:0000335 +name: substance quantification evidence +def: "A type of direct assay evidence where the quantity of a substance used as part of an assay is measured." [ECO:MCC] +comment: Two examples of substance quantification assay are (i) measuring how much of a radiolabelled compound is taken up by a cell and (ii) measuring how much of a substrate is turned over by a given enzyme. +is_a: ECO:0000002 ! direct assay evidence +created_by: mchibucos +creation_date: 2012-07-31T03:52:46Z + +[Term] +id: ECO:0000336 +name: competitive growth assay evidence +def: "A type of cell proliferation assay evidence in which a mutated strain of a microorganism, such as a yeast or bacterium, is grown competitively with wild-type cells and the relative fitness of the strains is assessed usually by means of a fluorescent marker and flow cytometric analysis." [GOC:MAH, PMID:14718172, PMID:20537132] +synonym: "fitness profiling" EXACT [GOC:MAH, PMID:20537132] +is_a: ECO:0005007 ! cell proliferation assay evidence +created_by: mchibucos +creation_date: 2012-08-24T12:03:58Z + +[Term] +id: ECO:0000337 +name: gel electrophoresis evidence +alt_id: ECO:0005026 +def: "A type of direct assay evidence where molecules have been sorted according to their size and charge by moving through a gel in the presence of an electric field." [ECO:MCC, wikipedia:Gel_electrophoresis] +comment: Molecules typically separated include DNA, RNA, or protein. The gel is typically made of agarose, polyacrylamide, or starch. +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000112 "We analyzed the electrophoretic pattern of cytosolic proteins extracted from LM3 and LM3-2 cells, grown under standard conditions up to mid-exponential phase (Fig. 1). The presence of differentially expressed proteins in the two strains, together with results of previous studies [28,29] demonstrated the role of CcpA as global regulator in L. plantarum." xsd:string {xref="PMID:17129387"} +created_by: mchibucos +creation_date: 2012-08-24T12:11:51Z + +[Term] +id: ECO:0000338 +name: pulsed-field gel electrophoresis evidence +def: "A type of gel electrophoresis evidence in which large DNA molecules are separated on agarose gel by alternately pulsed, perpendicularly oriented electrical fields, at least one of which is inhomogeneous." [PMID:6373014] +synonym: "PFGE" EXACT [] +is_a: ECO:0000337 ! gel electrophoresis evidence +created_by: mchibucos +creation_date: 2012-08-24T12:34:01Z + +[Term] +id: ECO:0000339 +name: two-dimensional agarose gel electrophoresis evidence +def: "A type of gel electrophoresis evidence where DNA molecules are electrophoresed on a low percentage agarose gel followed by high voltage electrophoresis on a higher percentage agarose gel in the presence of ethidium bromide." [PMID:16118435, PMID:8594382] +comment: The first dimension gel is intentionally run at low voltage in low percentage agarose to separate DNA molecules in proportion to their mass. The second dimension is run at high voltage in a gel of higher agarose concentration in the presence of ethidium bromide so that the mobility of a non-linear molecule is drastically influenced by its shape. +synonym: "2-D agarose gel electrophoresis" EXACT [] +is_a: ECO:0000337 ! gel electrophoresis evidence +created_by: mchibucos +creation_date: 2012-08-24T12:42:37Z + +[Term] +id: ECO:0000340 +name: plasmid maintenance assay evidence +def: "A type of experimental evidence in which a plasmid that is capable of being replicated is introduced into cells, cells are grown without selection for several generations, and retention of the plasmid is determined." [] {comment="ECO:MCC", comment="PMID:6323245"} +comment: Loss per generation is determined by comparing colony numbers on selective and non-selective media. +synonym: "minichromosome maintenance assay evidence" EXACT [PMID:6323245] +is_a: ECO:0000006 ! experimental evidence +created_by: mchibucos +creation_date: 2012-08-24T01:27:58Z + +[Term] +id: ECO:0000341 +name: specific protein inhibition by antibody evidence +def: "A type of specific protein inhibition evidence where the molecular function of a protein is inhibited by an antibody." [ECO:MCC] +is_a: ECO:0000020 ! protein inhibition evidence +created_by: mchibucos +creation_date: 2012-08-24T01:48:24Z + +[Term] +id: ECO:0000342 +name: support of intron positions by RNA-sequencing alignment evidence +def: "A type of RNA-seq evidence where intron locations in predicted transcripts are compared to intron locations supported by RNA-seq evidence." [ECO:MCC] +comment: RNA-seq alignment intron location evidence is used by NCBI RefSeq (http://www.ncbi.nlm.nih.gov/refseq). Exon-exon boundaries (or intron locations) of a RefSeq transcript, as determined by aligning the transcript to a reference chromosome, are compared to intron locations supported by RNAseq data. +synonym: "Support of intron positions by RNA-seq alignment evidence" EXACT [] +is_a: ECO:0000295 ! RNA-sequencing evidence +created_by: mchibucos +creation_date: 2013-04-03T04:22:05Z + +[Term] +id: ECO:0000343 +name: full support of intron positions by RNA-sequencing alignment evidence +def: "A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignment from a single sample supports all of the intron positions (exon pairs) predicted for a transcript." [ECO:MCC] +comment: Full support entails that all exon pairs represented in the transcript are supported. For example, for a transcript containing five exons, if liver RNA-seq reads support all four introns but brain RNA-seq supports only three introns, then the liver data fully support the transcript exons whereas the brain data do not. +synonym: "Full support of intron positions by RNA-seq alignment evidence" EXACT [] +is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence +created_by: mchibucos +creation_date: 2013-04-03T04:41:48Z + +[Term] +id: ECO:0000344 +name: partial support of intron positions by RNA-sequencing alignment evidence +def: "A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignment to a genome supports only some of the intron positions (exon pairs) predicted for a transcript." [ECO:MCC] +comment: Partial support can occur in two ways. First, one or more exon-exon pair (intron) is not supported by any RNA-seq sample included in the analysis. Second, no single RNA-seq sample provides support for all exon-exon pairs (introns) represented in a transcript, but all represented exons are supported by at least one sample. +synonym: "Partial support of intron positions by RNA-seq alignment evidence" EXACT [] +is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence +created_by: mchibucos +creation_date: 2013-04-03T04:50:46Z + +[Term] +id: ECO:0000345 +name: single exon transcript confirmation via alignment evidence +def: "A type of sequence alignment evidence resulting from the comparison of a single exon transcript to a collection / database of known single exon genes." [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897596/] +is_a: ECO:0000200 ! sequence alignment evidence +created_by: mchibucos +creation_date: 2013-04-03T05:10:34Z + +[Term] +id: ECO:0000346 +name: support of intron positions by RNA-sequencing alignment evidence used in manual assertion +def: "A type of support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion." [ECO:MCC] +synonym: "Support of intron positions by RNA-seq alignment evidence used in manual assertion" EXACT [] +is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence +is_a: ECO:0006068 ! RNA-sequencing evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2013-04-04T04:37:35Z + +[Term] +id: ECO:0000347 +name: support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +def: "A type of support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." [ECO:MCC] +synonym: "Support of intron positions by RNA-seq alignment evidence used in automatic assertion" EXACT [] +is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence +is_a: ECO:0006069 ! RNA-sequencing evidence used in automatic assertion +created_by: mchibucos +creation_date: 2013-04-04T04:38:45Z + +[Term] +id: ECO:0000348 +name: full support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +def: "A type of full support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." [ECO:MCC] +synonym: "Full support of intron positions by RNA-seq alignment evidence used in automatic assertion" EXACT [] +is_a: ECO:0000343 ! full support of intron positions by RNA-sequencing alignment evidence +is_a: ECO:0000347 ! support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +created_by: mchibucos +creation_date: 2013-04-04T04:40:19Z + +[Term] +id: ECO:0000349 +name: full support of intron positions by RNA-sequencing alignment evidence used in manual assertion +def: "A type of full support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion." [ECO:MCC] +synonym: "Full support of intron positions by RNA-seq alignment evidence used in manual assertion" EXACT [] +is_a: ECO:0000343 ! full support of intron positions by RNA-sequencing alignment evidence +is_a: ECO:0000346 ! support of intron positions by RNA-sequencing alignment evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2013-04-04T04:41:34Z + +[Term] +id: ECO:0000350 +name: partial support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +def: "A type of partial support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." [ECO:MCC] +synonym: "Partial support of intron positions by RNA-seq alignment evidence used in automatic assertion" EXACT [] +is_a: ECO:0000344 ! partial support of intron positions by RNA-sequencing alignment evidence +is_a: ECO:0000347 ! support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +created_by: mchibucos +creation_date: 2013-04-04T04:42:53Z + +[Term] +id: ECO:0000351 +name: partial support of intron positions by RNA-sequencing alignment evidence used in manual assertion +def: "A type of partial support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion." [ECO:MCC] +synonym: "Partial support of intron positions by RNA-seq alignment evidence used in manual assertion" EXACT [] +is_a: ECO:0000344 ! partial support of intron positions by RNA-sequencing alignment evidence +is_a: ECO:0000346 ! support of intron positions by RNA-sequencing alignment evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2013-04-04T04:43:56Z + +[Term] +id: ECO:0000352 +name: evidence used in manual assertion +def: "A type of evidence that is used in an manual assertion." [ECO:MCC] +is_a: ECO:0000000 {is_inferred="true"} ! evidence +created_by: mchibucos + +[Term] +id: ECO:0000353 +name: physical interaction evidence used in manual assertion +def: "A type of physical interaction evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_chemical_entity +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +synonym: "inferred from physical interaction" EXACT [GOECO:IPI] +synonym: "IPI" EXACT [GOECO:IPI] +xref: GOECO:IPI "inferred from physical interaction" +is_a: ECO:0000021 ! physical interaction evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ipi-inferred-physical-interaction xsd:string +created_by: mchibucos +creation_date: 2013-10-31T21:01:27Z + +[Term] +id: ECO:0000354 +name: gene neighbors evidence used in manual assertion +def: "A type of gene neighbors evidence that is used in a manual assertion." [ECO:MCC] +synonym: "ICL" RELATED [] +synonym: "inferred from genome cluster" BROAD [] +xref: GO_REF:0000025 "operon structure as IGC evidence" +is_a: ECO:0000084 ! gene neighbors evidence +is_a: ECO:0000317 ! genomic context evidence used in manual assertion +property_value: ECO:9000002 "IGC" xsd:string +created_by: mchibucos +creation_date: 2013-11-19T13:37:24Z + +[Term] +id: ECO:0000355 +name: phylogenetic distribution evidence +def: "A type of phylogenetic evidence characterized by the mapping of the character states of living organisms onto phylogenies using the method of maximum parsimony." [ECO:MCC, PMID:21238344] +comment: Example of use: "The presence of paired evaginated hemispheres and olfactory bulbs in both agnathan and gnathostome radiations suggests that such hemispheres were also present in the common ancestor." PMID: 7013637. Northcutt RG. (1981) Evolution of the telencephalon in nonmammals. Ann. Rev. Neurosci. +is_a: ECO:0000080 ! phylogenetic evidence +created_by: mchibucos +creation_date: 2014-04-22T17:02:31Z + +[Term] +id: ECO:0000358 +name: differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) +def: "A type of transcript expression evidence resulting from a microarray analysis with the use of the Gene Set Enrichment Analysis (GSEA) method or the Fisher's-Exact (FE) method to assess the differential expression." [PMID:19725948] +is_a: ECO:0000009 ! transcript expression evidence +created_by: mchibucos +creation_date: 2014-09-26T16:25:47Z + +[Term] +id: ECO:0000359 +name: differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) +def: "A type of transcript expression evidence resulting from the use of the Gene Set Enrichment Analysis (GSEA) method or Fisher's-Exact (FE) method to assess an RNA-seq dataset for differential gene expression." [PMC:2881125] +is_a: ECO:0000009 ! transcript expression evidence +created_by: mchibucos +creation_date: 2014-09-26T16:26:48Z + +[Term] +id: ECO:0000360 +name: biological target-disease association via drug evidence +def: "A type of experimental evidence resulting from the prediction of drug-target interactions by computational means." [https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-018-2199-x] +is_a: ECO:0000006 ! experimental evidence +created_by: mchibucos +creation_date: 2014-09-26T16:39:11Z + +[Term] +id: ECO:0000361 +name: inferential evidence +def: "A type of evidence where an assertion is derived from another assertion via logical inference or some non-logical but rational means." [ECO:MCC] +comment: Inference is the process of deriving logical conclusions from premises known or assumed to be true, or alternatively, arriving at conclusions via some non-logical means of observation of patterns of facts, which can result in identifying new meanings and contexts for understanding. Given the evidence provided by a set of premises, deductive reasoning involves drawing conclusions that necessarily follow, i.e. are certain, whereas inductive reasoning generates conclusions that are likely to be, but are not necessarily, true. +is_a: ECO:0000000 ! evidence +created_by: mchibucos +creation_date: 2015-07-14T11:19:37Z + +[Term] +id: ECO:0000362 +name: computational inference +def: "A type of inferential evidence based on a computationally derived unary inference or an inference chain in which at least one step is \ncomputationally derived." [ECO:MCC, GOC:MC] +synonym: "evidence based on computational logical inference" EXACT [] +is_a: ECO:0000361 ! inferential evidence +is_a: ECO:0007672 ! computational evidence +created_by: mchibucos +creation_date: 2015-07-14T11:24:27Z + +[Term] +id: ECO:0000363 +name: computational inference used in automatic assertion +def: "A type of evidence based on computational logical inference that is used in automatic assertion." [ECO:MCC] +synonym: "evidence based on computational logical inference used in automatic assertion" EXACT [] +xref: GO_REF:0000108 "Automatic assignment of GO terms using logical inference, based on on inter-ontology links" +is_a: ECO:0000362 ! computational inference +is_a: ECO:0007669 ! computational evidence used in automatic assertion +is_a: ECO:0007832 ! inferential evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2015-07-14T11:35:08Z + +[Term] +id: ECO:0000364 +name: evidence based on logical inference from manual annotation used in automatic assertion +def: "A type of evidence based on logical inference from a manually curated annotation that is used in an automatic assertion." [ECO:MCC] +is_a: ECO:0000363 ! computational inference used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2015-07-14T11:44:19Z + +[Term] +id: ECO:0000366 +name: evidence based on logical inference from automatic annotation used in automatic assertion +def: "A type of evidence based on logical inference from an automatically curated annotation that is used in an automatic assertion." [ECO:MCC] +is_a: ECO:0000363 ! computational inference used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2015-07-14T11:45:25Z + +[Term] +id: ECO:0000501 +name: evidence used in automatic assertion +def: "A type of evidence that is used in an automatic assertion." [ECO:cjm] +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +is_a: ECO:0000000 {is_inferred="true"} ! evidence +property_value: ECO:9000002 "IEA" xsd:string +property_value: seeAlso http://geneontology.org/page/automatically-assigned-evidence-codes xsd:string +created_by: cjm + +[Term] +id: ECO:0001001 +name: 3D cell culture evidence +def: "A type of cell proliferation assay evidence resulting from the growth of cells in a micro-environment that mimics real tissue and establishes physiological cell-cell and cell-substrate interactions that regulate proliferation and differentiation." [PMID:21042962, url:http\://volttecnologia.com.br/wp-content/uploads/2016/03/Drug-discovery.pdf] +is_a: ECO:0005007 ! cell proliferation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001002 +name: [3H]arachidonic acid release assay evidence +def: "A type of cell-based assay evidence resulting from labeling cells with [3H]arachidonic acid and subsequently determining the release of the arachidonic acid by the cells." [ISBN:0323152295, PMID:20693009] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001003 +name: [3H]-thymidine incorporation assay evidence +def: "A type of DNA synthesis cell proliferation assay evidence resulting from the measurement of cell proliferation rate by determining the incorporation of [3H]-thymidine into cellular nucleic acids." [OBI:0000669, url:http\://www.scientistsolutions.com/forum/cell-culture-and-tissue-culture-proliferationapoptosis/3h-thymidine-incorporation-assay-rat-1a] +is_a: ECO:0005008 ! DNA synthesis cell proliferation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001004 +name: 51Cr release assay evidence +def: "A type of cytotoxicity assay evidence resulting from the measurement of the amount of radioactive 51Cr released in the supernatant of a sample of 51Cr pre-labeled cells via cytolysis by effector cells (cytotoxic T-cells)." [PMC:3929704, url:http\://www4.mpbio.com/ecom/docs/proddata.nsf/5f64ffd4f38c2fda8525645d00769d68/53d2a75653615bab852568cb00572ff3?OpenDocument] +is_a: ECO:0005012 ! cytotoxicity assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001005 +name: 7-aminoactinomycin staining evidence +def: "A type of apoptotic assay evidence resulting from the use of 7-aminoactinomycin D (7-AAD) to distinguish viable, apoptotic, and dead cells, using the fact that permeability of the cell membrane, and fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells." [ECO:RCT] +synonym: "7-AAD staining evidence" EXACT [] +synonym: "7-amino-actinomycin D staining evidence" EXACT [] +is_a: ECO:0005019 ! staining evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001006 +name: adhesion assay evidence +def: "A type of cell-based assay evidence resulting from the analysis of cell binding either to extracellular matrix proteins or other cells." [PMID:15576904, PMID:21909903] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001007 +name: adoptive cell transfer evidence +def: "A type of ex vivo assay evidence derived by ex vivo selection of tumor-reactive lymphocytes, and their activation and numerical expansion before re-infusion to the autologous tumor-bearing host." [ECO:SN, url:http\://jco.ascopubs.org/content/23/10/2346.full.pdf+html, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf] +synonym: "Adoptive immunotherapy" EXACT [] +synonym: "passive immunization" EXACT [] +is_a: ECO:0005502 ! ex vivo assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001008 +name: alamarBlue assay evidence +def: "A type of cell viability assay evidence resulting from the continuous measurement of fluorescence intensity from the viable cells, where a cell permeable compound, resazurin (the active ingredient of alamarBlue), is reduced to fluorescent resorufin." [PMC:3478843, url:https\://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/cell-profilteration-assay-protocols/cell-viability-with-alamarblue.html] +is_a: ECO:0005004 ! cell viability assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001009 +name: allograft transplantation phenotypic evidence +def: "A type of anatomical perturbation phenotypic evidence resulting from the transplantation of an organ or tissue from one individual of the same species with a different genotype." [ECO:SN, url:http\://www.nature.com/subjects/allograft, url:http\://www.organdonor.gov/about/terms_and_topics/] +synonym: "allograft transplantation experiment evidence" EXACT [] +synonym: "allografting" EXACT [] +synonym: "Allotransplantation" EXACT [] +synonym: "tissue grafting" NARROW [] +is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001010 +name: anion-exchange chromatography evidence +def: "A type of chromatography evidence where a positively charged ion exchange resin with an affinity for molecules having net negative surface charges is used to separate the molecules." [ECO:SN, PMID:20978968, url:http\://www.bio-rad.com/en-us/applications-technologies/anion-exchange-chromatography, url:http\://www.uta.edu/faculty/sawasthi/Enzymology-4351-5324/Class%20Syllabus%20Enzymology/Ion%20Exchange%20Chromatography-1.pdf] +is_a: ECO:0000325 ! chromatography evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001011 +name: annexin-V staining evidence +def: "A type of apoptotic assay evidence resulting from the addition and subsequent binding of Annexin V to phosphatidylserine in cells to detect if they are viable, apoptotic, or necrotic." [PMC:3169266] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001012 +name: cognitive assay phenotypic evidence +def: "A type of experimental phenotypic evidence resulting from behavioral phenotyping and analysis to assess cognitive functioning." [PMID:24462904] +synonym: "behavioral assay evidence" EXACT [] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001013 +name: blocking monoclonal antibody evidence +def: "A type of immunological assay evidence resulting from inhibitory effect of monoclonal antibody combining with an antigen over other antibodies." [PMID:11292349, url:http\://www.sciencedirect.com/science/article/pii/S0140673600034966] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001014 +name: blocking peptide evidence +def: "A type of immunological assay evidence resulting from the use of peptides to block antibodies from binding to their targets." [ECO:RCT] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001015 +name: blocking polyclonal antibody evidence +def: "A type of immunological assay evidence resulting from the inhibitory effect of polyclonal antibodies (antibodies secreted by different B cell lineages), which while not reacting with a specific antigen, serve to prevent other antibodies from doing so." [] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001016 +name: blood test evidence +def: "A type of direct assay evidence where a blood sample is extracted from an organism to analyze different blood components." [ECO:SN, url:https\://www.nhlbi.nih.gov/health/health-topics/topics/bdt] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001017 +name: Boyden chamber assay evidence +def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by measuring the number of cells that migrate from the upper compartment of a chamber (separated by a micro-porous membrane) to the lower compartment, in which chemotactic agents are present." [PMID:13872176, PMID:15576901] +is_a: ECO:0005021 ! chemotaxis assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001018 +name: bromodeoxyuridine incorporation assay evidence +alt_id: ECO:0005010 +def: "A type of nucleotide analog incorporation evidence resulting from the incorporation of bromodeoxyuridine (BrdU) as a thymidine analog into nuclear DNA, resulting in a label that can be tracked using antibody probes." [PMID:23690005] +synonym: "5-bromo-2'-deoxyuridine incorporation assay evidence" EXACT [] +synonym: "BrdU incorporation assay evidence" EXACT [] +synonym: "BrdUrd incorporation assay evidence" EXACT [] +synonym: "BUdR incorporation assay evidence" EXACT [] +synonym: "cell proliferation marker detection assay evidence" BROAD [] +is_a: ECO:0001107 ! nucleotide analog incorporation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001019 +name: caspase assay evidence +def: "A type of apoptotic assay evidence resulting from the detection of caspase activation, which results in the cleaving of intracellular substrates during apoptosis." [PMID:18314058, PMID:25086023] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001020 +name: cell counting evidence +def: "A type of cell-based assay evidence resulting from the quantification of a sample of cells." [ECO:RCT] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001021 +name: cell permeability assay evidence +def: "A type of cell-based assay evidence resulting from the quantification of cell permeability." [PMID:16962665] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001022 +name: carboxyfluorescein diacetate succinimidyl ester staining evidence +def: "A type of staining evidence resulting from monitoring division by the use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to label intracellular molecules with carboxyfluorescein; when a labeled cell divides, the number of carboxyfluorescein-tagged molecules is split." [PMC:3185625] +synonym: "CFDA-SE staining evidence" EXACT [] +synonym: "CFSE-staining evidence" EXACT [] +is_a: ECO:0005019 ! staining evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001023 +name: chemiluminescence-linked immunoassay evidence +def: "A type of protein detection assay evidence resulting from a complex (a chemiluminescent compound, protein and a steroidal hapten) utilized as a labeled antigen, which emits light when treated with hydrogen peroxide and copper acetate at a high pH." [PMID:659901] +synonym: "CLIA evidence" EXACT [] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001024 +name: chimeric protein phenotypic evidence +def: "A type of mutant phenotype evidence resulting from the fusion of two or more different genes to create one protein product." [PMID:22588898, PMID:25832756] +synonym: "chimeric protein evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001025 +name: co-electrophoresis evidence +def: "A type of gel electrophoresis evidence resulting from the electrophoresis of two substances together, allowing for the characterization of ligand/nucleic acid binding interactions." [PMID:7668395] +is_a: ECO:0000337 ! gel electrophoresis evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001026 +name: co-localization evidence +def: "A type of imaging assay evidence resulting from the limited observation of the co-occurrence of molecules in a subcellular location." [PMC:3074624] +is_a: ECO:0000324 ! imaging assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001027 +name: colony counting evidence +def: "A type of direct assay evidence resulting from the counting of microbial colonies that arise from viable cells grown on a plate or dish." [PMID:16558698, PMID:23457446] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001028 +name: co-sedimentation assay evidence +def: "A type of physical interaction evidence resulting from the separation of a mixture of molecules under the influence of a force such as artificial gravity, where molecules sedimenting together are assumed to interact." [MI:0027] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001029 +name: comet assay evidence +def: "A type of gel electrophoresis evidence resulting in the assessment of DNA breakage in a cell, based on size and shape of DNA migration." [OBI:0302736, PMID:10737956] +is_a: ECO:0000337 ! gel electrophoresis evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001030 +name: conditional knockout evidence +def: "A type of knockout evidence resulting from a temporally-restricted and/or tissue-specific targeted gene removal." [PMC:3572410] +is_a: ECO:0001091 ! knockout phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001031 +name: conditional knockin evidence +def: "A type of knockin evidence resulting from the targeted introduction of a temporally-restricted and/or tissue-specific mutation." [PMID:16832820] +is_a: ECO:0001090 ! knockin evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001032 +name: constitutively active mutant evidence +def: "A type of experimental phenotypic evidence resulting from a constitutively active mutant (CAM), resulting in mutant proteins that remain active in the absence of upstream signals." [PMID:12217490] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001033 +name: cross-linking evidence +def: "A type of protein binding evidence resulting from the identification of two interacting proteins (that exist in close proximity) by linking through covalent bonds followed by identification" [PMID:15530987, PMID:19241040] +is_a: ECO:0000024 ! protein binding evidence +property_value: IAO:0000112 "To confirm this intriguing observation, we cross-linked AdpA and DNA in the presence of increasing amounts of ATP (figure 2b(iii)). In this assay, ATP prevented the formation of AdpA - DNA complexes in a concentration-dependent manner, an outcome consistent with the results obtained by SPR analysis." xsd:string {xref="PMID:22870392"} +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001034 +name: crystallography evidence +def: "A type of structure determination evidence resulting from the use of a narrow beam of x-rays to identify molecular structures by creating a diffraction pattern." [MI:0114] +is_a: ECO:0005031 ! structure determination evidence +property_value: IAO:0000112 "The crystal structures of E. coli MarR and Enterococcus faecalis SlyA-like proteins show their cross-sections are ~70 angstroms, suggesting they would protect ~20 bp of DNA (Alekshun et al., 2001; Wu et al., 2003). This suggests the presence of two SlyA dimers at the SlyA I site and at least three dimers at the SlyA II site." xsd:string {xref="PMID:17892462"} +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001035 +name: cytochemistry evidence +def: "A type of histochemistry evidence resulting from localizing chemical components of cells and organelles on histological sections." [PMID:11597006] +is_a: ECO:0001050 ! histochemistry evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001036 +name: cytochrome C release assay evidence +def: "A type of apoptotic assay evidence resulting from the identification of an accumulation of cytochrome C in the cytoplasm after its release from the mitochondria, an early event in apoptosis." [PMID:10914021, PMID:12815469] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001037 +name: 4',6-diamidino-2-phenylindole staining evidence +def: "A type of staining evidence resulting from 4',6-diamidino-2-phenylindole (DAPI) binding to AT regions of DNA and emitting a blue fluorescence." [PMID:8580206, url:https\://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/protocols/dapi-imaging-protocol.html] +synonym: "DAPI staining evidence" EXACT [] +synonym: "diaminophenylindole staining" EXACT [] +is_a: ECO:0005019 ! staining evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001038 +name: deletion mutation phenotypic evidence +alt_id: ECO:0005510 +def: "A type of mutant phenotype evidence resulting from a mutation in which there is the removal of one or more contiguous nucleotides that may result in altered function or other measurable properties." [SO:0000159] +comment: As a child of 'experimental phenotypic evidence', this is not to be confused with the biological processes deletion mutation / deletion deficiency in which DNA is lost during DNA replication. +comment: Contrast with knockout phenotypic evidence in which any number of changes to the DNA result in elimination of the function of the gene in question. +comment: The length of the DNA deleted can range from a single nucleotide to enough material that could include multiple genes. +synonym: "deletion mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000112 "In contrast, and in full agreement with the previous results of Wade et al. (17), the longer deletion in the TB23 fragment, that removes MelR binding site 2, results in a sharp reduction in the repression of the melR promoter by MelR." xsd:string {xref="PMID:18346968"} +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001039 +name: DNA laddering assay evidence +def: "A type of apoptotic assay evidence resulting from the visualization via gel electrophoresis of the fragmented DNA (DNA ladder) which is the result of apoptotic DNA fragmentation." [PMC:4401164] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001040 +name: RNA dot blot assay evidence +def: "A type of RNA detection assay evidence based on the direct application of a RNA mixture in a circular motion on a matrix for hybridization with labeled DNA fragments." [ECO:SW, PMID:21424648] +synonym: "RNA dot blot" RELATED [] +is_a: ECO:0005518 ! RNA detection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001042 +name: dominant-negative mutant phenotypic evidence +def: "A type of mutant phenotype evidence resulting from a mutated gene producing mutant polypeptides that disrupt the activity of the wild-type genes." [PMC:2217636, PMID:8018332] +synonym: "dominant-negative mutant evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001043 +name: Edman degradation evidence used in manual assertion +def: "A type of Edman degradation evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001044 ! Edman degradation evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:59:00Z + +[Term] +id: ECO:0001044 +name: Edman degradation evidence +def: "A type of protein detection assay evidence resulting from labeling a termonal amino acid residue and cleaving it from a peptide by sequentially removing one residue at a time from the amino end of the peptide, without disrupting the bonds." [doi:10.3891/acta.chem.scand.04-0283, OBI:0000705] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001045 +name: eTag assay evidence +def: "A type of affinity evidence resulting from the quantitative analysis of gene and protein expression monitored by proprietary eTag reporters." [PMID:15137949] +is_a: ECO:0000023 ! affinity evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001046 +name: filter binding assay evidence +def: "A type of physical interaction evidence resulting from a mixture of two molecules passed through a nitrocellulose filter, where one may be immobilized on the filter, and if the immobilized molecule is capable of binding to the other, it will be retained on the filter as well." [PMID:23028069] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001047 +name: fluorescence in situ hybridization evidence +def: "A type of nucleic acid localization evidence and in situ hybridization evidence resulting from the use of fluorescent probes to detect complementary sequences of nucleic acids." [PMC:346675, PMID:6812046] +is_a: ECO:0001534 ! nucleic acid localization evidence +is_a: ECO:0001836 ! in situ hybridization evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001048 +name: fluorescence resonance energy transfer evidence +def: "A type of dynamic fluorescence quenching evidence resulting from the measurement of the proximity of two fluorophores, where the energy from an excited molecular fluorophore to another fluorophore can only occur within ~10nm." [PMID:11516318, PMID:15696158] +synonym: "FRET" EXACT [] +is_a: ECO:0006273 ! dynamic fluorescence quenching evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001049 +name: gel-filtration evidence +def: "A type of chromatography evidence resulting from the exclusion of proteins based on molecular size by filtration through a porous matrix (swollen gel), from which larger molecules are excluded." [CHMO:0001011, doi:10.1038/nmeth0506-410] +is_a: ECO:0000325 ! chromatography evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001050 +name: histochemistry evidence +def: "A type of histology evidence resulting from the identification of chemical components in cells and tissues." [MMO:0000497] +is_a: ECO:0001051 ! histology evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001051 +name: histology evidence +def: "A type of imaging assay evidence resulting from the qualitative microscopic examination of cells or tissues." [OBI:0600020] +is_a: ECO:0000324 ! imaging assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001052 +name: high-performance liquid chromatography evidence +def: "A type of chromatography evidence resulting from the separation of molecules in a solute by forcing the solvent containing the sample through a column packed with nonporous particles at high pressure." [PMID:16376355] +synonym: "HPLC evidence" EXACT [] +is_a: ECO:0000325 ! chromatography evidence +property_value: IAO:0000112 "No OD increases with the mutant and the wild type were measured with DMSO provided as electron acceptor at 2 and 10 mM; however, HPLC analyses of cultures with 2 mM DMSO revealed that DMSO was completely consumed in wild type cultures, whereas no DMSO consumption was evident in the mutant cultures (Figure 3)." xsd:string {xref="PMID:21450087"} +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001053 +name: immunocytochemistry evidence +def: "A type of protein detection assay evidence resulting from the use of antibodies linked to coloring agents to localize structures in cell cultures to identify proteins." [MI:1200, OBI:0001986] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001054 +name: immunodepletion evidence +def: "A type of immunological assay evidence resulting from the use of antibodies to remove specific proteins from a sample." [BAO:0002505] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001055 +name: immunohistochemistry evidence +def: "A type of protein detection assay evidence resulting from the use of antibodies to detect proteins in localized cells of tissue sections." [OBI:0001986] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001056 +name: induced mutation evidence +def: "A type of genetic transformation evidence resulting from a mutation induced by a mutagenic compounds or irradiation." [OBI:0001154] +comment: This term was made obsolete for a couple of reasons: the def. doesn’t make sense as the suggested means of mutagenesis fail to introduce exogenous DNA (it is a child of genetic transformation evidence) and the def. is too much like random mutagenesis evidence. +property_value: IAO:0000234 SIB:PG xsd:string +is_obsolete: true +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001057 +name: in vitro acetylation assay evidence +def: "A type of acetylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] +is_a: ECO:0001546 ! acetylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001058 +name: in vitro cleavage assay evidence +def: "A type of cleavage assay evidence used in an in vitro experiment." [PMID:21121091] +is_a: ECO:0001547 ! cleavage assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001059 +name: in vitro deubiquitination assay evidence +def: "A type of deubiquitination assay evidence used in an in vitro experiment." [ECO:SN, PMID:19692941, SIB:PG] +is_a: ECO:0001552 ! deubiquitination assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001060 +name: in vitro deacetylation assay evidence +def: "A type of deacetylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] +is_a: ECO:0001548 ! deacetylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001061 +name: in vitro defarnesylation assay evidence +def: "A type of defarnesylation assay evidence used in an in vitro experiment." [ECO:SN, PMID:16126733, SIB:PG] +is_a: ECO:0001549 ! defarnesylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001062 +name: in vitro demethylation assay evidence +def: "A type of demethylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Demethylation] +is_a: ECO:0001550 ! demethylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001063 +name: in vitro desumoylation assay evidence +def: "A type of desumoylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] +is_a: ECO:0001551 ! desumoylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001064 +name: in vitro farnesylation assay evidence +def: "A type of farnesylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Farnesyltransferase] +is_a: ECO:0001553 ! farnesylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001065 +name: in vitro methylation assay evidence +def: "A type of methylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Methylation] +is_a: ECO:0001554 ! methylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001066 +name: in vitro palmitoylation assay evidence +def: "A type of palmitoylation assay evidence used in an in vitro experiment." [ECO:SN, PMID:17077383, SIB:PG] +is_a: ECO:0001555 ! palmitoylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001067 +name: in vitro phosphatase assay evidence +def: "A type of phosphatase assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Phosphatase] +is_a: ECO:0001556 ! phosphatase assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001068 +name: in vitro protein kinase assay evidence +def: "A type of protein kinase assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Protein_kinase, url:http\://en.wikipedia.org/wiki/Protein_phosphorylation] +is_a: ECO:0001558 ! protein kinase assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001069 +name: in vitro polyADP-ribosylation assay evidence +def: "A type of polyADP-ribosylation assay evidence used in an in vitro experiment." [ECO:SN, PMID:21870253, SIB:PG] +is_a: ECO:0001557 ! polyADP-ribosylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001070 +name: in vitro sumoylation assay evidence +def: "A type of sumoylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] +is_a: ECO:0001559 ! sumoylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001071 +name: in vitro transcription assay evidence +def: "A type of transcription assay evidence used in an in vitro experiment." [ECO:SN, ECO:SW, PMID:21125481, SIB:PG] +is_a: ECO:0000168 ! transcription assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001072 +name: in vitro translation assay evidence +def: "A type of translation assay evidence used in an in vitro experiment." [ECO:SN, PMID:18230759, SIB:PG] +is_a: ECO:0001561 ! translation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001073 +name: in vitro ubiquitination assay evidence +def: "A type of ubiquitination assay evidenceused in an in vitro experiment." [ECO:SN, PMID:19692941, SIB:PG] +is_a: ECO:0001562 ! ubiquitination assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001074 +name: in vivo acetylation assay evidence +def: "A type of acetylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] +is_a: ECO:0001546 ! acetylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001075 +name: in vivo cleavage assay evidence +def: "A type of cleavage assay evidence used in an in vivo experiment." [ECO:SN, PMID:22154596] +is_a: ECO:0001547 ! cleavage assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001076 +name: in vivo deacetylation assay evidence +def: "A type of deacetylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] +is_a: ECO:0001548 ! deacetylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001077 +name: in vivo defarnesylation assay evidence +def: "A type of defarnesylation assay evidence used in an in vivo experiment." [ECO:SN, PMID:15556768, PMID:16507103, SIB:PG] +is_a: ECO:0001549 ! defarnesylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001078 +name: in vivo demethylation assay evidence +def: "A type of demethylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Demethylation] +is_a: ECO:0001550 ! demethylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001079 +name: in vivo deubiquitination assay evidence +def: "A type of deubiquitination assay evidence used in an in vivo experiment." [ECO:SN, PMID:19692941, SIB:PG] +is_a: ECO:0001552 ! deubiquitination assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001080 +name: in vivo desumoylation assay evidence +def: "A type of desumoylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] +is_a: ECO:0001551 ! desumoylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001081 +name: in vivo farnesylation assay evidence +def: "A type of farnesylation assay evidence used in an in vivo experiment." [ECO:SN, PMID:9030603, SIB:PG] +is_a: ECO:0001553 ! farnesylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001082 +name: in vivo methylation assay evidence +def: "A type of methylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Methylation] +is_a: ECO:0001554 ! methylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001083 +name: in vivo palmitoylation assay evidence +def: "A type of palmitoylation assay evidence used in an in vivo experiment." [ECO:SN, PMID:10329400, SIB:PG] +is_a: ECO:0001555 ! palmitoylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001084 +name: in vivo phosphatase assay evidence +def: "A type of phosphatase assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Phosphatase] +is_a: ECO:0001556 ! phosphatase assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001085 +name: in vivo protein kinase assay evidence +def: "A type of protein kinase assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Protein_kinase, url:http\://en.wikipedia.org/wiki/Protein_phosphorylation] +is_a: ECO:0001558 ! protein kinase assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001086 +name: in vivo sumoylation assay evidence +def: "A type of sumoylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] +is_a: ECO:0001559 ! sumoylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001087 +name: in vivo transcription assay evidence +def: "A type of transcription assay evidence used in an in vivo experiment." [ECO:SN, PMID:12181418, SIB:PG] +is_a: ECO:0000168 ! transcription assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001088 +name: in vivo translation assay evidence +def: "A type of translation assay evidence used in an in vivo experiment." [ECO:SN, PMID:24901308, SIB:PG] +is_a: ECO:0001561 ! translation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001089 +name: in vivo ubiquitination assay evidence +def: "A type of ubiquitination assay evidence used in an in vivo experiment." [ECO:SN, PMID:19692941, SIB:PG] +is_a: ECO:0001562 ! ubiquitination assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001090 +name: knockin evidence +def: "A type of genetic transformation evidence resulting from the targetted replacement of a wild-type DNA sequence with a different sequence." [PMID:18077807, PMID:21800101] +is_a: ECO:0005027 ! genetic transformation evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001091 +name: knockout phenotypic evidence +def: "A type of genetic transformation evidence resulting from a change in the DNA that results in the elimination of the function of the gene, allowing for functional analysis." [PMC:2782548] +comment: A change in the DNA may result from any number of processes such (deletion, insertion, frame shift, etc.) Additionally, the gene may be made inoperative via homologous recombination, site-specific nucleases, zinc finger nucleases, transcription activator-like effector nucleases (TALENs), or CRISPR (Clustered regularly interspaced short palindromic repeats). +comment: Contrast with deletion mutation phenotypic evidence in which DNA is deleted which may result in altering the function of the resulting protein(s). +synonym: "gene deletion evidence" NARROW [] +synonym: "gene knockout evidence" NARROW [] +synonym: "knockout evidence" EXACT [] +is_a: ECO:0005027 ! genetic transformation evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001092 +name: lipid binding assay evidence +def: "A type of physical interaction evidence resulting from the qualitative and quantitative analysis of the affinity with which a protein binds to a lipid." [PMID:22848065, PMID:23681540] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001093 +name: luminescence-based mammalian interactome mapping assay evidence +def: "A type of physical interaction evidence resulting from the analysis of protein-protein interactions by use of a luciferase enzyme fused to a particular protein (or proteins), which are coexpressed with epitope-tagged partners in mammalian cells." [MI:0729, PMID:15761153] +synonym: "LUMIER assay" EXACT [] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001094 +name: macroscopy evidence +def: "A type of experimental evidence that is visible to the naked eye cf. microscopy evidence which requires the aid of a microscope." [] +is_a: ECO:0000006 ! experimental evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001095 +name: mammalian 2-hybrid assay evidence +def: "A type of bait-prey hybrid interaction evidence resulting from the analysis of proteins of interest attached to two portions of the transcriptional activator, and in interaction bring the two portions together to increase expression of the reporter gene." [ECO:RCT, PMID:9043710] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001096 +name: mass spectrometry evidence +def: "A type of spectrometry evidence resulting from identifying the amount and type of material entities present in a sample by fragmenting it and measuring the mass-to-charge ratio of the resulting particles." [OBI:0000470] +is_a: ECO:0005504 ! spectrometry evidence +property_value: IAO:0000112 "The MS analyses showed that two VapB10 peaks (836.437, 1584.789 m/z) and three VapC10-His6 peaks (1049.526, 1299.733 and 1455.853 m/z) were detected in MS-DIGEST program, indicating that both VapB10 and VapC10-His6 had the expected peptide masses (Figure S3). These results suggest that VapB10 binds to VapC10-His6 forming the TA complex VapBC10 in vivo, which may cause the counteraction of the VapC10-induced growth arrest (Figure 2)." xsd:string {xref="PMID:24260461"} +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001097 +name: medical imaging evidence +def: "A type of imaging assay evidence resulting from the use of technology to visualize and provide information about the body to diagnose, treat, or monitor medical conditions." [url:http\://www.fda.gov/Radiation-EmittingProducts/RadiationEmittingProductsandProcedures/MedicalImaging/ucm2005914.htm] +is_a: ECO:0000324 ! imaging assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001098 +name: microscopy evidence +def: "A type of direct assay evidence resulting from the use of a microscope." [ECO:MCC] +comment: Microscopy may include any of the following: optical, electron, scanning probe, or X-ray. +is_a: ECO:0000324 ! imaging assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001099 +name: motility wound healing assay evidence +alt_id: ECO:0001135 +def: "A type of cell-based assay evidence to measure cell motility in which a \"wound\" is created in a cell monolayer and the migration of the cells is captured by imaging at regular intervals during cell migration to close the wound." [] +synonym: "wound healing assay evidence" BROAD [] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001100 +name: MTS assay evidence +def: "A type of apoptotic assay evidence resulting from the reduction of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) in combination with phenazine methyl sulfate (PMS) as an intermediate electron acceptor reagent to produce a soluble formazan dye in viable cells." [NBK:144065, PMID:1867954] +synonym: "3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium" NARROW [] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001101 +name: MTT assay evidence +def: "A type of apoptotic assay evidence resulting from the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to its insoluble purple formazan dye in viable cells and measures cell viability / metabolic activity." [NBK:144065, PMID:6606682] +synonym: "3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide" NARROW [] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001102 +name: multiplex bead-based immunoassay evidence +def: "A type of protein detection assay evidence resulting from determination of analyte concentration by fluorescent bead sets attached to an antibody (to a specific analyte) with a fluorescent reporter dye label attached to a second antibody (to a specific analyte)." [PMC:1534009] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001103 +name: natural variation mutant evidence +def: "A type of experimental phenotypic evidence resulting from the observation of the impact of a natural mutation on the expressed traits." [ECO:RCT] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001104 +name: nuclear fragmentation evidence +def: "A type of apoptotic assay evidence resulting from the nuclei fragmenting into smaller pieces during programmed cell death." [PMID:16738861] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001105 +name: nuclear magnetic resonance evidence +def: "A type of magnetic resonance evidence in which atomic nuclei in a strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting electromagnetic signal is captured." [url:https\://en.wikipedia.org/wiki/Nuclear_magnetic_resonance] +is_a: ECO:0006252 ! magnetic resonance evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001106 +name: nuclease protection assay evidence +def: "A type of RNA detection assay evidence resulting from the detection and quantitation of specific RNAs (from a sample of total cellular RNA) that are bound by antisense RNA or DNA probes and therefore protected from nucleases." [PMID:17486122, PMID:18428580] +is_a: ECO:0005518 ! RNA detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001107 +name: nucleotide analog incorporation assay evidence +def: "A type of DNA synthesis cell proliferation assay evidence resulting from the analysis and measurement of the incorporation of fluorescently labeled nucleotide analog(s) into the DNA." [PMC:3149870] +is_a: ECO:0005008 ! DNA synthesis cell proliferation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001108 +name: phage display evidence +def: "A type of protein binding evidence resulting from the panning of a phage library where the individual phage displayed a different peptide or protein by fusion to coat proteins on the capsid." [MI:0084, PMID:11680867] +is_a: ECO:0000024 ! protein binding evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001109 +name: phosphoamino acid analysis evidence +def: "A type of direct assay evidence resulting from the identification of the phosphorylated residue in a protein by amino acid analysis." [PMID:11680867, PMID:18429115] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001110 +name: peptide affinity enrichment evidence +def: "A type of affinity evidence resulting from the appending of affinity tags on proteins so that they may be purified from their biological source using an affinity technique." [] +synonym: "phosphopeptide affinity enrichment evidence" NARROW [] +is_a: ECO:0000023 ! affinity evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001111 +name: physical examination evidence +def: "A type of direct assay evidence resulting from the physical examination and measurement of the feature of a subject or sample." [ECO:RCT] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001112 +name: peptide array evidence +def: "A type of protein binding evidence resulting from protein-bound peptides detected from a collection of peptides arranged as an array and incubated with the partner protein in order to map protein-protein interaction sites." [PMID:21243154] +synonym: "phosphopeptide array evidence" NARROW [] +is_a: ECO:0000024 ! protein binding evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001113 +name: point mutation phenotypic evidence +def: "A type of mutant phenotype evidence resulting from the change in a single nucleotide." [SO:1000008] +synonym: "point mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001114 +name: propidium iodide staining evidence +def: "A type of staining evidence resulting from the binding and labeling of DNA (during apoptosis, when there is a loss of nuclear DNA content) with propidium iodide (PI) to identify the cells from which it originated." [PMID:17406435] +is_a: ECO:0005019 ! staining evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001115 +name: fluorescence evidence +def: "A type of direct assay evidence resulting from the analysis of a molecule by its intrinsic fluorescence, or by attaching it with a fluorophore." [url:http\://www.nature.com/subjects/biological-fluorescence] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001116 +name: protein dot blot assay evidence +def: "A type of protein detection assay evidence resulting from protein samples immobilized on a membrane, then subsequently incubated and imaged." [doi:10.1007/978-94-009-0951-9_24] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001117 +name: protein microarray evidence +def: "A type of protein detection assay evidence resulting from proteins immobilized to prepare protein chips, or microwells, that can be utilized to determine presence of proteins, monitor differential expression profiles, and/or study protein interactions." [PMC:1828913] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001118 +name: protein sequencing assay evidence +def: "A type of sequencing assay evidence resulting from determining the sequence of amino acids in a protein." [ERO:0001287] +is_a: ECO:0000220 ! sequencing assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001119 +name: quantitative mass spectrometry evidence +def: "A type of mass spectrometry evidence resulting from quantitative analysis of peptides, proteins, and proteomes." [PMID:22665296] +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001120 +name: radioisotope assay evidence +def: "A type of direct assay evidence resulting from the assessment of biological reactions by use of a radioactive isotope to label the reactant." [ISBN:978-3-642-50036-7] +synonym: "radioassay" EXACT [] +synonym: "radioisotopic assay" EXACT [] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001121 +name: radioimmunoassay evidence +def: "A type of protein detection assay evidence resulting from the use of a radioligand to measure the binding of a substance to a specific antibody or other receptor system." [MeSH:D011863] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001123 +name: resonant mirror biosensor evidence +def: "A type of direct assay evidence from the analysis of the real-time interaction between an analyte in solution and its interactant linked to the surface of the resonant mirror." [PMID:19151938] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001124 +name: restriction fragment detection evidence +def: "A type of DNA detection assay evidence resulting from homologous DNA fragments digested by restriction enzymes, and the resulting restriction fragments are sorted by length to illustrate differences." [doi:10.1007/978-94-009-0951-9_24, url:http\://www.ncbi.nlm.nih.gov/probe/docs/techrflp/] +is_a: ECO:0005519 ! DNA detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001126 +name: spectrophotometry evidence +def: "A type of spectrometry evidence resulting from the evaluation of a molecule in a fluid or solid by its ability to alter the transmission of light at specific wavelengths." [MMO:0000173] +is_a: ECO:0005504 ! spectrometry evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001127 +name: surface plasmon resonance evidence +def: "A type of physical interaction evidence based on real-time, rapid plasmon generation on the interface between a planar surface and vacuum that measures changes in refractive index close to the sensor surface when an analyte and its immobilized ligand bind to observe and characterize molecular interaction." [ECO:SW, PMID:11578932, PMID:19151937, PMID:8574707] +comment: Plasmons are generated by an incident light beam. +synonym: "SPR evidence" EXACT [PMID:11578932] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000112 "Electrophoretic mobility shift assay (EMSA) and surface plasmon resonance (SPR) demonstrated that AdpAHis6 specifically bound the oriC fragment (283-bp) containing the in silico-predicted A-boxes in a concentration-dependent manner (figures 1b and 2a), but not the remaining part of oriC (data not shown)." xsd:string {xref="PMID:22870392"} +property_value: IAO:0000112 "Further confirmation of the specific interaction was obtained by conducting the competing surface plasmon resonance (SPR) assay with the unlabeled DNA fragments. As shown in Additional file 3, a significantly lower response was observed when either the unlabeled S2 or S5 was added together with MtrA, which indicated that they could compete the binding of MtrA with the promoter DNA on the chip." xsd:string {xref="PMID:20843371"} +property_value: IAO:0000112 "Our SPR analysis revealed that also housekeeping genes required for ribosome function (rplR) and beta subunit RNA polymerase (rpoB) belong to the LexA regulon, a feature of the SOS network not yet observed in bacteria." xsd:string {xref="PMID:24713082"} +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001128 +name: syngeneic transplantation experiment evidence +def: "A type of experimental phenotypic evidence resulting from a transplantation in which the transplanted material (stem cells) is from an individual's identical twin." [PMID:18469352] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001129 +name: TACE activity assay evidence +def: "A type of enzymatic activity assay evidence resulting from the analysis of tumor necrosis factor (TNF)-a converting enzyme (TACE), which releases a soluble TNF-a from the membrane-bound precursor protein." [PMC:1808921] +synonym: "Tumor necrosis factor - alpha converting enzyme activity assay" EXACT [] +is_a: ECO:0000005 ! enzymatic activity assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001130 +name: tissue microarray evidence +def: "A type of cytochemistry evidence resulting from the analysis of section(s) of a paraffin block containing embedded tissues cores arranged in an array pattern." [PMID:11770905] +is_a: ECO:0001035 ! cytochemistry evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001131 +name: transgenic organism evidence +def: "A type of genetic transformation evidence resulting from an organism that has had its expressed phenotype altered by modification." [OBI:1000048] +is_a: ECO:0005027 ! genetic transformation evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001132 +name: tryptic phosphopeptide mapping assay evidence +def: "A type of mass spectrometry evidence resulting from the analysis of prepared phosphopeptides separated in two dimensions on a TLC plate." [PMID:18429120] +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001133 +name: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence +def: "A type of apoptotic assay evidence resulting from the visualization of DNA fragmentation by detection of exposed 3'-OH ends, localized by terminal deoxynucleotidyl transferase (TdT) which then catalyzes the addition of labeled dUTPs." [PMID:22566045] +synonym: "TdT-mediated dUTP-biotin nick end labeling assay" EXACT [] +synonym: "terminal deoxynucleotidyl transferase-dUTP nick end labeling assay evidence" EXACT [] +synonym: "TUNEL assay evidence" EXACT [] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001134 +name: urine test evidence +def: "A type of direct assay evidence resulting from the analysis of a urine sample." [ECO:RCT] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001136 +name: WST-1 assay evidence +alt_id: ECO:0005009 +def: "A type of cell proliferation assay evidence resulting from the assessment of metabolic activity from a water-soluble tetrazolium salt WST-1 being reduced outside the cell (by reacting with mitochondrial succinate-tetrazolium reductase) to form a formazan dye." [PMID:18417231] +synonym: "metabolic cell proliferation assay evidence" BROAD [] +is_a: ECO:0005007 ! cell proliferation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001137 +name: xenotransplantation phenotypic evidence +def: "A type of anatomical perturbation phenotypic evidence resulting from the transplantation, implantation, or infusion of live cells, tissues, or organs between individuals of different species." [ECO:SN, url:http\://ilarjournal.oxfordjournals.org/content/37/1/16.full.pdf+html, url:http\://www.fda.gov/BiologicsBloodVaccines/Xenotransplantation/] +synonym: "tissue grafting" NARROW [] +synonym: "Xenograft transplantation" EXACT [] +synonym: "Xenografting" EXACT [] +synonym: "Xenotransplantation" EXACT [] +synonym: "xenotransplantation experiment evidence" EXACT [] +is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001138 +name: 3D cell culture evidence used in manual assertion +def: "A type of 3D cell culture evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001001 ! 3D cell culture evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:18:35Z + +[Term] +id: ECO:0001139 +name: 51Cr release assay evidence used in manual assertion +def: "A type of 51Cr release assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001004 ! 51Cr release assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:20:55Z + +[Term] +id: ECO:0001140 +name: 7-aminoactinomycin staining evidence used in manual assertion +def: "A type of 7-aminoactinomycin staining evidence that is used in a manual assertion." [ECO:MCC] +synonym: "7-AAD staining evidence" BROAD [] +synonym: "7-amino-actinomycin D staining evidence" BROAD [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001005 ! 7-aminoactinomycin staining evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:22:01Z + +[Term] +id: ECO:0001141 +name: [3H]-thymidine incorporation assay evidence used in manual assertion +def: "A type of [3H]-thymidine incorporation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001003 ! [3H]-thymidine incorporation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:31:52Z + +[Term] +id: ECO:0001142 +name: [3H]arachidonic acid release assay evidence used in manual assertion +def: "A type of [3H]arachidonic acid release assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001002 ! [3H]arachidonic acid release assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:32:44Z + +[Term] +id: ECO:0001143 +name: adhesion assay evidence used in manual assertion +def: "A type of adhesion assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001006 ! adhesion assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:33:38Z + +[Term] +id: ECO:0001144 +name: adoptive cell transfer evidence used in manual assertion +def: "A type of adoptive cell transfer evidence that is used in a manual assertion." [ECO:MCC] +synonym: "Adoptive immunotherapy" BROAD [] +synonym: "passive immunization" BROAD [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001007 ! adoptive cell transfer evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:36:19Z + +[Term] +id: ECO:0001145 +name: alamarBlue assay evidence used in manual assertion +def: "A type of alamarBlue assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001008 ! alamarBlue assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:37:21Z + +[Term] +id: ECO:0001146 +name: allograft transplantation phenotypic evidence used in manual assertion +def: "A type of allograft transplantation phenotypic evidence that is used in a manual assertion." [ECO:MCC] +synonym: "allograft transplantation evidence used in manual assertion" EXACT [] +synonym: "allografting" BROAD [] +synonym: "Allotransplantation" BROAD [] +synonym: "tissue grafting" RELATED [] +is_a: ECO:0001009 ! allograft transplantation phenotypic evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:38:22Z + +[Term] +id: ECO:0001147 +name: anion-exchange chromatography evidence used in manual assertion +def: "A type of anion-exchange chromatography evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001010 ! anion-exchange chromatography evidence +is_a: ECO:0007680 ! chromatography evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:44:30Z + +[Term] +id: ECO:0001148 +name: annexin-V staining evidence used in manual assertion +def: "A type of annexin-V staining evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001011 ! annexin-V staining evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:52:01Z + +[Term] +id: ECO:0001149 +name: cognitive assay phenotypic evidence used in manual assertion +def: "A type of cognitive assay phenotypic evidence used that is used in a manual assertion." [ECO:MCC] +synonym: "behavioral assay evidence used in manual assertion" EXACT [] +is_a: ECO:0001012 ! cognitive assay phenotypic evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:53:38Z + +[Term] +id: ECO:0001150 +name: blocking monoclonal antibody evidence used in manual assertion +def: "A type of blocking monoclonal antibody evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001013 ! blocking monoclonal antibody evidence +is_a: ECO:0006078 ! immunological assay evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:54:44Z + +[Term] +id: ECO:0001151 +name: blocking peptide evidence used in manual assertion +def: "A type of blocking peptide evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001014 ! blocking peptide evidence +is_a: ECO:0006078 ! immunological assay evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:55:33Z + +[Term] +id: ECO:0001152 +name: blocking polyclonal antibody evidence used in manual assertion +def: "A type of blocking polyclonal antibody evidence tthat is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001015 ! blocking polyclonal antibody evidence +is_a: ECO:0006078 ! immunological assay evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:01:39Z + +[Term] +id: ECO:0001153 +name: blood test evidence used in manual assertion +def: "A type of blood test evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001016 ! blood test evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:02:47Z + +[Term] +id: ECO:0001154 +name: Boyden chamber assay evidence used in manual assertion +def: "A type of Boyden chamber assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001017 ! Boyden chamber assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:03:53Z + +[Term] +id: ECO:0001155 +name: bromodeoxyuridine incorporation assay evidence used in manual assertion +def: "A type of bromodeoxyuridine incorporation assay evidence used in manual assertion." [ECO:MCC] +synonym: "5-bromo-2'-deoxyuridine incorporation assay evidence" BROAD [] +synonym: "BrdU incorporation assay evidence" BROAD [] +synonym: "BrdUrd incorporation assay evidence" BROAD [] +synonym: "BUdR incorporation assay evidence" BROAD [] +is_a: ECO:0001018 ! bromodeoxyuridine incorporation assay evidence +is_a: ECO:0001241 ! nucleotide analog incorporation assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:04:49Z + +[Term] +id: ECO:0001156 +name: caspase assay evidence used in manual assertion +def: "A type of caspase assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001019 ! caspase assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:06:06Z + +[Term] +id: ECO:0001157 +name: cell counting evidence used in manual assertion +def: "A type of cell counting evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001020 ! cell counting evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:07:13Z + +[Term] +id: ECO:0001158 +name: cell permeability assay evidence used in manual assertion +def: "A type of cell permeability assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001021 ! cell permeability assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:07:58Z + +[Term] +id: ECO:0001159 +name: carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion +def: "A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in a manual assertion." [ECO:MCC] +synonym: "CFDA-SE staining evidence" BROAD [] +synonym: "CFSE-staining evidence" BROAD [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001022 ! carboxyfluorescein diacetate succinimidyl ester staining evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:08:54Z + +[Term] +id: ECO:0001160 +name: chemiluminescence-linked immunoassay evidence used in manual assertion +def: "A type of chemiluminescence-linked immunoassay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "CLIA evidence" BROAD [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001023 ! chemiluminescence-linked immunoassay evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:09:55Z + +[Term] +id: ECO:0001161 +name: chimeric protein phenotypic evidence used in manual assertion +def: "A type of chimeric protein phenotypic evidence that is used in a manual assertion." [ECO:MCC] +synonym: "chimeric protein evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0001024 ! chimeric protein phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:10:40Z + +[Term] +id: ECO:0001162 +name: co-electrophoresis evidence used in manual assertion +def: "A type of co-electrophoresis evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001025 ! co-electrophoresis evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:11:22Z + +[Term] +id: ECO:0001163 +name: co-localization evidence used in manual assertion +def: "A type of co-localization evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001026 ! co-localization evidence +is_a: ECO:0001256 ! imaging assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:12:04Z + +[Term] +id: ECO:0001164 +name: co-sedimentation assay evidence used in manual assertion +def: "A type of co-sedimentation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +is_a: ECO:0001028 ! co-sedimentation assay evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:12:55Z + +[Term] +id: ECO:0001165 +name: colony counting evidence used in manual assertion +def: "A type of colony counting evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001027 ! colony counting evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:14:57Z + +[Term] +id: ECO:0001166 +name: comet assay evidence used in manual assertion +def: "A type of comet assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001029 ! comet assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:16:01Z + +[Term] +id: ECO:0001167 +name: conditional knockin evidence used in manual assertion +def: "A type of conditional knockin evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001031 ! conditional knockin evidence +is_a: ECO:0001224 ! knockin evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:17:22Z + +[Term] +id: ECO:0001168 +name: conditional knockout evidence used in manual assertion +def: "A type of conditional knockout evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001030 ! conditional knockout evidence +is_a: ECO:0001225 ! knockout evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:17:58Z + +[Term] +id: ECO:0001169 +name: constitutively active mutant evidence used in manual assertion +def: "A type of constitutively active mutant evidence that is used a in manual assertion." [ECO:MCC] +is_a: ECO:0001032 ! constitutively active mutant evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:20:23Z + +[Term] +id: ECO:0001170 +name: cross-linking evidence used in manual assertion +def: "A type of cross-linking evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001033 ! cross-linking evidence +is_a: ECO:0006076 ! protein binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:21:29Z + +[Term] +id: ECO:0001171 +name: crystallography evidence used in manual assertion +def: "A type of crystallography evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001034 ! crystallography evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:22:20Z + +[Term] +id: ECO:0001172 +name: cytochemistry evidence used in manual assertion +def: "A type of cytochemistry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001035 ! cytochemistry evidence +is_a: ECO:0001185 ! histochemistry evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:23:10Z + +[Term] +id: ECO:0001173 +name: cytochrome C release assay evidence used in manual assertion +def: "A type of cytochrome C release assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001036 ! cytochrome C release assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:23:53Z + +[Term] +id: ECO:0001174 +name: 4',6-diamidino-2-phenylindole staining evidence used in manual assertion +def: "A type of 4',6-diamidino-2-phenylindole staining evidence that is used in a manual assertion." [ECO:MCC] +synonym: "DAPI staining evidence" BROAD [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001037 ! 4',6-diamidino-2-phenylindole staining evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:25:03Z + +[Term] +id: ECO:0001175 +name: deletion mutation phenotypic evidence used in manual assertion +def: "A type of deletion mutation phenotypic evidence that is used in a manual assertion." [ECO:MCC] +synonym: "deletion mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0001038 ! deletion mutation phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:25:54Z + +[Term] +id: ECO:0001176 +name: DNA laddering assay evidence used in manual assertion +def: "A type of DNA laddering assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001039 ! DNA laddering assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:26:46Z + +[Term] +id: ECO:0001177 +name: RNA dot blot assay evidence used in manual assertion +def: "A type of RNA dot blot assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "RNA dot blot evidence" BROAD [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001040 ! RNA dot blot assay evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:45:52Z + +[Term] +id: ECO:0001179 +name: dominant-negative mutant phenotypic evidence used in manual assertion +def: "A type of dominant-negative mutant phenotypic evidence that is used in a manual assertion." [ECO:MCC] +synonym: "dominant-negative mutant evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0001042 ! dominant-negative mutant phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:47:44Z + +[Term] +id: ECO:0001180 +name: eTag assay evidence used in manual assertion +def: "A type of eTag assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001045 ! eTag assay evidence +is_a: ECO:0006075 ! affinity evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:59:39Z + +[Term] +id: ECO:0001181 +name: filter binding assay evidence used in manual assertion +def: "A type of filter binding assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +is_a: ECO:0001046 ! filter binding assay evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:03:28Z + +[Term] +id: ECO:0001182 +name: fluorescence in situ hybridization evidence used in manual assertion +def: "A type of fluorescence in situ hybridization evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001047 ! fluorescence in situ hybridization evidence +is_a: ECO:0001838 ! in situ hybridization evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:04:20Z + +[Term] +id: ECO:0001183 +name: fluorescence resonance energy transfer evidence used in manual assertion +def: "A type of fluorescence resonance energy transfer evidence that is used in a manual assertion." [ECO:MCC] +synonym: "FRET evidence" BROAD [] +is_a: ECO:0001048 ! fluorescence resonance energy transfer evidence +is_a: ECO:0006327 ! dynamic fluorescence quenching evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:05:22Z + +[Term] +id: ECO:0001184 +name: gel-filtration evidence used in manual assertion +def: "A type of gel-filtration evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001049 ! gel-filtration evidence +is_a: ECO:0007680 ! chromatography evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:06:14Z + +[Term] +id: ECO:0001185 +name: histochemistry evidence used in manual assertion +def: "A type of histochemistry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001050 ! histochemistry evidence +is_a: ECO:0001187 ! histology evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:07:19Z + +[Term] +id: ECO:0001186 +name: immunocytochemistry evidence used in manual assertion +def: "A type of immunocytochemistry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001053 ! immunocytochemistry evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:12:39Z + +[Term] +id: ECO:0001187 +name: histology evidence used in manual assertion +def: "A type of histology evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001051 ! histology evidence +is_a: ECO:0001256 ! imaging assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:09:50Z + +[Term] +id: ECO:0001188 +name: immunodepletion evidence used in manual assertion +def: "A type of immunodepletion evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001054 ! immunodepletion evidence +is_a: ECO:0006078 ! immunological assay evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:16:19Z + +[Term] +id: ECO:0001189 +name: immunohistochemistry evidence used in manual assertion +def: "A type of immunohistochemistry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001055 ! immunohistochemistry evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:17:22Z + +[Term] +id: ECO:0001190 +name: in vitro acetylation assay evidence used in manual assertion +def: "A type of in vitro acetylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001057 ! in vitro acetylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:19:24Z + +[Term] +id: ECO:0001191 +name: in vitro cleavage assay evidence used in manual assertion +def: "A type of in vitro cleavage assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001058 ! in vitro cleavage assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:21:22Z + +[Term] +id: ECO:0001192 +name: in vitro deacetylation assay evidence used in manual assertion +def: "A type of in vitro deacetylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001060 ! in vitro deacetylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:23:42Z + +[Term] +id: ECO:0001193 +name: in vitro defarnesylation assay evidence used in manual assertion +def: "A type of in vitro defarnesylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001061 ! in vitro defarnesylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:25:45Z + +[Term] +id: ECO:0001194 +name: in vitro demethylation assay evidence used in manual assertion +def: "A type of in vitro demethylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001062 ! in vitro demethylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:27:00Z + +[Term] +id: ECO:0001195 +name: in vitro desumoylation assay evidence used in manual assertion +def: "A type of in vitro desumoylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001063 ! in vitro desumoylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:29:01Z + +[Term] +id: ECO:0001196 +name: in vitro deubiquitination assay evidence used in manual assertion +def: "A type of in vitro deubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001059 ! in vitro deubiquitination assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:29:48Z + +[Term] +id: ECO:0001197 +name: in vitro farnesylation assay evidence used in manual assertion +def: "A type of in vitro farnesylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001064 ! in vitro farnesylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:30:50Z + +[Term] +id: ECO:0001198 +name: in vitro methylation assay evidence used in manual assertion +def: "A type of in vitro methylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001065 ! in vitro methylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:31:54Z + +[Term] +id: ECO:0001199 +name: in vitro palmitoylation assay evidence used in manual assertion +def: "A type of in vitro palmitoylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001066 ! in vitro palmitoylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:32:57Z + +[Term] +id: ECO:0001200 +name: in vitro phosphatase assay evidence used in manual assertion +def: "A type of in vitro phosphatase assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001067 ! in vitro phosphatase assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:34:16Z + +[Term] +id: ECO:0001201 +name: in vitro polyADP-ribosylation assay evidence used in manual assertion +def: "A type of in vitro polyADP-ribosylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001069 ! in vitro polyADP-ribosylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:34:57Z + +[Term] +id: ECO:0001202 +name: in vitro protein kinase assay evidence used in manual assertion +def: "A type of in vitro protein kinase assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001068 ! in vitro protein kinase assay evidence +is_a: ECO:0005801 ! enzymatic activity assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:39:57Z + +[Term] +id: ECO:0001203 +name: in vitro sumoylation assay evidence used in manual assertion +def: "A type of in vitro sumoylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001070 ! in vitro sumoylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:41:25Z + +[Term] +id: ECO:0001204 +name: in vitro transcription assay evidence used in manual assertion +def: "A type of in vitro transcription assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001071 ! in vitro transcription assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:44:40Z + +[Term] +id: ECO:0001205 +name: in vitro translation assay evidence used in manual assertion +def: "A type of in vitro translation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001072 ! in vitro translation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:46:30Z + +[Term] +id: ECO:0001206 +name: in vitro ubiquitination assay evidence used in manual assertion +def: "A type of in vitro ubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001073 ! in vitro ubiquitination assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:47:18Z + +[Term] +id: ECO:0001207 +name: in vivo acetylation assay evidence used in manual assertion +def: "A type of in vivo acetylation assay evidence that is used in manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001074 ! in vivo acetylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:48:56Z + +[Term] +id: ECO:0001208 +name: in vivo cleavage assay evidence used in manual assertion +def: "A type of in vivo cleavage assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001075 ! in vivo cleavage assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:49:43Z + +[Term] +id: ECO:0001209 +name: in vivo deacetylation assay evidence used in manual assertion +def: "A type of in vivo deacetylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001076 ! in vivo deacetylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:50:47Z + +[Term] +id: ECO:0001210 +name: in vivo defarnesylation assay evidence used in manual assertion +def: "A type of in vivo defarnesylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001077 ! in vivo defarnesylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:52:41Z + +[Term] +id: ECO:0001211 +name: in vivo demethylation assay evidence used in manual assertion +def: "A type of in vivo demethylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001078 ! in vivo demethylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:53:40Z + +[Term] +id: ECO:0001212 +name: in vivo desumoylation assay evidence used in manual assertion +def: "A type of in vivo desumoylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001080 ! in vivo desumoylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:54:36Z + +[Term] +id: ECO:0001213 +name: in vivo deubiquitination assay evidence used in manual assertion +def: "A type of in vivo deubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001079 ! in vivo deubiquitination assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:56:28Z + +[Term] +id: ECO:0001214 +name: in vivo farnesylation assay evidence used in manual assertion +def: "A type of in vivo farnesylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001081 ! in vivo farnesylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:57:18Z + +[Term] +id: ECO:0001215 +name: in vivo methylation assay evidence used in manual assertion +def: "A type of in vivo methylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001082 ! in vivo methylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:03:16Z + +[Term] +id: ECO:0001216 +name: in vivo palmitoylation assay evidence used in manual assertion +def: "A type of in vivo palmitoylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001083 ! in vivo palmitoylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:04:07Z + +[Term] +id: ECO:0001217 +name: in vivo phosphatase assay evidence used in manual assertion +def: "A type of in vivo phosphatase assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001084 ! in vivo phosphatase assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:06:08Z + +[Term] +id: ECO:0001218 +name: in vivo protein kinase assay evidence used in manual assertion +def: "A type of in vivo protein kinase assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001085 ! in vivo protein kinase assay evidence +is_a: ECO:0005801 ! enzymatic activity assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:07:58Z + +[Term] +id: ECO:0001219 +name: in vivo sumoylation assay evidence used in manual assertion +def: "A type of in vivo sumoylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001086 ! in vivo sumoylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:09:49Z + +[Term] +id: ECO:0001220 +name: in vivo transcription assay evidence used in manual assertion +def: "A type of in vivo transcription assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001087 ! in vivo transcription assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:11:20Z + +[Term] +id: ECO:0001221 +name: in vivo translation assay evidence used in manual assertion +def: "A type of in vivo translation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001088 ! in vivo translation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:42:50Z + +[Term] +id: ECO:0001222 +name: in vivo ubiquitination assay evidence used in manual assertion +def: "A type of in vivo ubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001089 ! in vivo ubiquitination assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:44:11Z + +[Term] +id: ECO:0001223 +name: induced mutation evidence used in manual assertion +def: "A type of induced mutation evidence that is used in a manual assertion." [ECO:MCC] +property_value: IAO:0000234 SIB:PG xsd:string +is_obsolete: true +created_by: mchibucos +creation_date: 2014-03-17T18:45:56Z + +[Term] +id: ECO:0001224 +name: knockin evidence used in manual assertion +def: "A type of knockin evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001090 ! knockin evidence +is_a: ECO:0006049 ! genetic transformation evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:49:35Z + +[Term] +id: ECO:0001225 +name: knockout evidence used in manual assertion +def: "A type of knockout evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001091 ! knockout phenotypic evidence +is_a: ECO:0006049 ! genetic transformation evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:51:31Z + +[Term] +id: ECO:0001226 +name: lipid binding assay evidence used in manual assertion +def: "A type of lipid binding assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +is_a: ECO:0001092 ! lipid binding assay evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:52:19Z + +[Term] +id: ECO:0001227 +name: luminescence-based mammalian interactome mapping assay evidence used in manual assertion +def: "A type of LUMIER assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "LUMIER assay evidence" BROAD [] +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +is_a: ECO:0001093 ! luminescence-based mammalian interactome mapping assay evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:55:16Z + +[Term] +id: ECO:0001228 +name: macroscopy evidence used in manual assertion +def: "A type of macroscopy evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001094 ! macroscopy evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:56:21Z + +[Term] +id: ECO:0001229 +name: mammalian 2-hybrid assay evidence used in manual assertion +def: "A type of mammalian 2-hybrid assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001095 ! mammalian 2-hybrid assay evidence +is_a: ECO:0006077 ! bait-prey hybrid interaction evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:57:25Z + +[Term] +id: ECO:0001230 +name: mass spectrometry evidence used in manual assertion +def: "A type of mass spectrometry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:59:50Z + +[Term] +id: ECO:0001231 +name: medical imaging evidence used in manual assertion +def: "A type of medical imaging evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001097 ! medical imaging evidence +is_a: ECO:0001256 ! imaging assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:04:43Z + +[Term] +id: ECO:0001232 +name: microscopy evidence used in manual assertion +def: "A type of microscopy evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001098 ! microscopy evidence +is_a: ECO:0001256 ! imaging assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:05:37Z + +[Term] +id: ECO:0001233 +name: motility wound healing assay evidence used in manual assertion +alt_id: ECO:0001262 +def: "A type of motility wound healing assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "wound healing assay evidence used in manual assertion" BROAD [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001099 ! motility wound healing assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:08:13Z + +[Term] +id: ECO:0001234 +name: MTS assay evidence used in manual assertion +def: "A type of MTS assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium" RELATED [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001100 ! MTS assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:08:51Z + +[Term] +id: ECO:0001235 +name: MTT assay evidence used in manual assertion +def: "A type of MTT assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide" RELATED [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001101 ! MTT assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:13:17Z + +[Term] +id: ECO:0001236 +name: multiplex bead-based immunoassay evidence used in manual assertion +def: "A type of multiplex bead-based immunoassay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001102 ! multiplex bead-based immunoassay evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:14:03Z + +[Term] +id: ECO:0001237 +name: natural variation mutant evidence used in manual assertion +def: "A type of natural variation mutant evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001103 ! natural variation mutant evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:15:48Z + +[Term] +id: ECO:0001238 +name: nuclear magnetic resonance evidence used in manual assertion +def: "A type of nuclear magnetic resonance evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001105 ! nuclear magnetic resonance evidence +is_a: ECO:0006285 ! magnetic resonance evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:18:50Z + +[Term] +id: ECO:0001239 +name: nuclear fragmentation evidence used in manual assertion +def: "A type of nuclear fragmentation evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001104 ! nuclear fragmentation evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:19:55Z + +[Term] +id: ECO:0001240 +name: nuclease protection assay evidence used in manual assertion +def: "A type of nuclease protection assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001106 ! nuclease protection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:23:41Z + +[Term] +id: ECO:0001241 +name: nucleotide analog incorporation assay evidence used in manual assertion +def: "A type of nucleotide analog incorporation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001107 ! nucleotide analog incorporation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:25:17Z + +[Term] +id: ECO:0001242 +name: phage display evidence used in manual assertion +def: "A type of phage display evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001108 ! phage display evidence +is_a: ECO:0006076 ! protein binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:27:17Z + +[Term] +id: ECO:0001243 +name: phosphoamino acid analysis evidence used in manual assertion +def: "A type of phosphoamino acid analysis evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001109 ! phosphoamino acid analysis evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:28:59Z + +[Term] +id: ECO:0001244 +name: peptide affinity enrichment evidence used in manual assertion +def: "A type of peptide affinity enrichment evidence that is used in a manual assertion." [ECO:MCC] +synonym: "phosphopeptide affinity enrichment evidence" RELATED [] +is_a: ECO:0001110 ! peptide affinity enrichment evidence +is_a: ECO:0006075 ! affinity evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:30:13Z + +[Term] +id: ECO:0001245 +name: peptide array evidence used in manual assertion +def: "A type of peptide array evidence that is used in a manual assertion." [ECO:MCC] +synonym: "phosphopeptide array evidence" RELATED [] +is_a: ECO:0001112 ! peptide array evidence +is_a: ECO:0006076 ! protein binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:16:45Z + +[Term] +id: ECO:0001246 +name: physical examination evidence used in manual assertion +def: "A type of physical examination evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001111 ! physical examination evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:17:27Z + +[Term] +id: ECO:0001247 +name: point mutation phenotypic evidence used in manual assertion +def: "A type of point mutation phenotypic evidence that is used in a manual assertion." [ECO:MCC] +synonym: "point mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0001113 ! point mutation phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:18:10Z + +[Term] +id: ECO:0001248 +name: propidium iodide staining evidence used in manual assertion +def: "A type of propidium iodide staining evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001114 ! propidium iodide staining evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:18:58Z + +[Term] +id: ECO:0001249 +name: fluorescence evidence used in manual assertion +def: "A type of protein detection by fluorescence evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001115 ! fluorescence evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:19:35Z + +[Term] +id: ECO:0001250 +name: protein dot blot assay evidence used in manual assertion +def: "A type of protein dot blot assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001116 ! protein dot blot assay evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:20:30Z + +[Term] +id: ECO:0001251 +name: protein microarray evidence used in manual assertion +def: "A type of protein microarray evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001117 ! protein microarray evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:21:11Z + +[Term] +id: ECO:0001252 +name: protein sequencing assay evidence used in manual assertion +def: "A type of protein sequencing assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001118 ! protein sequencing assay evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:22:08Z + +[Term] +id: ECO:0001253 +name: quantitative mass spectrometry evidence used in manual assertion +def: "A type of quantitative mass spectrometry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001119 ! quantitative mass spectrometry evidence +is_a: ECO:0001230 ! mass spectrometry evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:23:15Z + +[Term] +id: ECO:0001254 +name: radioisotope assay evidence used in manual assertion +def: "A type of radioisotope assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "radioassay evidence" BROAD [] +synonym: "radioisotopic assay evidence" BROAD [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001120 ! radioisotope assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:27:05Z + +[Term] +id: ECO:0001255 +name: radioimmunoassay evidence used in manual assertion +def: "A type of radioimmunoassay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001121 ! radioimmunoassay evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:28:04Z + +[Term] +id: ECO:0001256 +name: imaging assay evidence used in manual assertion +def: "A type of imaging assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "radiologic test evidence" RELATED [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0000324 ! imaging assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:00Z + +[Term] +id: ECO:0001257 +name: restriction fragment detection evidence used in manual assertion +def: "A type of restriction fragment detection evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001124 ! restriction fragment detection evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:05Z + +[Term] +id: ECO:0001258 +name: spectrophotometry evidence used in manual assertion +def: "A type of spectrophotometry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001126 ! spectrophotometry evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:09Z + +[Term] +id: ECO:0001259 +name: syngeneic transplantation experiment evidence used in manual assertion +def: "A type of syngeneic transplantation experiment evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001128 ! syngeneic transplantation experiment evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:13Z + +[Term] +id: ECO:0001260 +name: xenotransplantation phenotypic evidence used in manual assertion +def: "A type of xenotransplantation phenotypic evidence that is used in a manual assertion." [ECO:MCC] +synonym: "tissue grafting" RELATED [] +synonym: "xenograft transplantation evidence" BROAD [] +synonym: "xenografting evidence" BROAD [] +synonym: "xenotransplantation evidence" BROAD [] +synonym: "xenotransplantation experiment evidence used in manual assertion" EXACT [] +is_a: ECO:0001137 ! xenotransplantation phenotypic evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:18Z + +[Term] +id: ECO:0001261 +name: WST-1 assay evidence used in manual assertion +def: "A type of WST-1 assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "metabolic cell proliferation assay evidence" BROAD [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001136 ! WST-1 assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:22Z + +[Term] +id: ECO:0001263 +name: urine test evidence used in manual assertion +def: "A type of urine test evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001134 ! urine test evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:30Z + +[Term] +id: ECO:0001264 +name: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion +def: "A type of terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "TdT-mediated dUTP-biotin nick end labeling assay evidence" BROAD [] +synonym: "terminal deoxynucleotidyl transferase-dUTP nick end labeling assay evidence" BROAD [] +synonym: "TUNEL assay evidence" BROAD [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001133 ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:34Z + +[Term] +id: ECO:0001265 +name: tryptic phosphopeptide mapping assay evidence used in manual assertion +def: "A type of tryptic phosphopeptide mapping assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001132 ! tryptic phosphopeptide mapping assay evidence +is_a: ECO:0001230 ! mass spectrometry evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:38Z + +[Term] +id: ECO:0001266 +name: transgenic organism evidence used in manual assertion +def: "A type of transgenic organism evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001131 ! transgenic organism evidence +is_a: ECO:0006049 ! genetic transformation evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:41Z + +[Term] +id: ECO:0001267 +name: tissue microarray evidence used in manual assertion +def: "A type of tissue microarray evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001130 ! tissue microarray evidence +is_a: ECO:0001172 ! cytochemistry evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:45Z + +[Term] +id: ECO:0001268 +name: TACE activity assay evidence used in manual assertion +def: "A type of TACE activity assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "Tumor necrosis factor - alpha converting enzyme activity assay evidence" BROAD [] +is_a: ECO:0001129 ! TACE activity assay evidence +is_a: ECO:0005801 ! enzymatic activity assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:49Z + +[Term] +id: ECO:0001269 +name: surface plasmon resonance evidence used in manual assertion +def: "A type of surface plasmon resonance evidence that is used in a manual assertion." [ECO:MCC] +synonym: "SPR evidence" BROAD [] +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +is_a: ECO:0001127 ! surface plasmon resonance evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:54Z + +[Term] +id: ECO:0001270 +name: restriction landmark genomic scanning evidence used in manual assertion +def: "A type of restriction landmark genome scanning evidence that is used in a manual assertion." [ECO:MCC] +synonym: "restriction landmark genome scanning evidence" BROAD [] +synonym: "RLGS evidence" BROAD [] +is_a: ECO:0000089 ! restriction landmark genomic scanning evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:58Z + +[Term] +id: ECO:0001271 +name: resonant mirror biosensor evidence used in manual assertion +def: "A type of resonant mirror biosensor evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001123 ! resonant mirror biosensor evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:30:02Z + +[Term] +id: ECO:0001272 +name: high-performance liquid chromatography evidence used in manual assertion +def: "A type of high-performance liquid chromatography evidence that is used in a manual assertion." [ECO:MCC] +synonym: "HPLC evidence" BROAD [] +is_a: ECO:0001052 ! high-performance liquid chromatography evidence +is_a: ECO:0007680 ! chromatography evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: mchibucos +creation_date: 2014-03-16T20:49:54Z + +[Term] +id: ECO:0001273 +name: ectopic expression evidence used in manual assertion +def: "A type of ectopic expression evidence that is used in a manual assertion." [ECO:MCC] +synonym: "analysis of overexpression/ectopic expression phenotype" RELATED [] +is_a: ECO:0000017 ! ectopic expression evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T22:34:56Z + +[Term] +id: ECO:0001522 +name: small molecule detection assay evidence +def: "A type of molecule detection assay evidence resulting from the detection and quantification of a small molecule (a low molecular weight (typically < 900 daltons) organic compound), such as lipids, sugars, animo acids, drugs, etc." [] +synonym: "metabolite detection assay evidence" NARROW [] +is_a: ECO:0005516 ! molecule detection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro + +[Term] +id: ECO:0001533 +name: localization evidence +def: "A type of direct assay evidence in which the sub-cellular location of a protein or nucleic acid sequence is determined." [] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_ExpGenomicCleanup" xsd:string +created_by: jbmunro +creation_date: 2018-05-17T12:00:00Z + +[Term] +id: ECO:0001534 +name: nucleic acid localization evidence +def: "A type of localization evidence based on localization of a specific segment of DNA or RNA within tissue by the application of a complementary strand of nucleic acid to which a reporter molecule (i.e. either radio-, fluorescent- or antigen-labeled probe) is attached and quantified using either autoradiography, fluorescence microscopy, or immunohistochemistry." [] +is_a: ECO:0001533 ! localization evidence +property_value: IAO:0000234 "ECO_ExpGenomicCleanup" xsd:string +created_by: jbmunro +creation_date: 2018-05-17T12:00:00Z + +[Term] +id: ECO:0001546 +name: acetylation assay evidence +def: "A type of direct assay evidence where acetylated residues are detected in a protein." [SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001547 +name: cleavage assay evidence +def: "A type of direct assay evidence where the cleavage of a protein into protein fragments by a protease is detected." [PMID:21121091, PMID:22154596] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001548 +name: deacetylation assay evidence +def: "A type of direct assay evidence where the removal of acetyl groups are detected in a protein." [SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001549 +name: defarnesylation assay evidence +def: "A type of direct assay evidence where the removal of farnesyl groups from a protein is detected." [PMID:15556768, PMID:16126733, PMID:16507103, SIB:PG] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001550 +name: demethylation assay evidence +def: "A type of direct assay evidence where the removal of methyl groups from a substrate (RNA/DNA or protein) is detected." [SIB:PG, url:http\://en.wikipedia.org/wiki/Demethylation] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001551 +name: desumoylation assay evidence +def: "A type of direct assay evidence where the removal of sumo groups from a protein is detected." [SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001552 +name: deubiquitination assay evidence +def: "A type of direct assay evidence where the removal of ubiquitin groups from a protein is detected." [PMID:19692941, SIB:PG] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001553 +name: farnesylation assay evidence +def: "A type of direct assay evidence where farnesylated residues in proteins are detected." [PMID:9030603, SIB:PG, url:http\://en.wikipedia.org/wiki/Farnesyltransferase] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001554 +name: methylation assay evidence +def: "A type of direct assay evidence where methylated residues of a substarte (RNA/DNA or protein) are detected." [SIB:PG, url:http\://en.wikipedia.org/wiki/Methylation] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001555 +name: palmitoylation assay evidence +def: "A type of direct assay evidence where palmitoylated residues in a protein are detected." [PMID:10329400, PMID:17077383, SIB:PG] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001556 +name: phosphatase assay evidence +def: "A type of direct assay evidence where the removal of phosphatase groups from a protein is detected." [SIB:PG, url:http\://en.wikipedia.org/wiki/Phosphatase] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001557 +name: polyADP-ribosylation assay evidence +def: "A type of direct assay evidence where ADP-ribosylated residues in proteins are detected." [PMID:21870253, PMID:2820766, SIB:PG] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001558 +name: protein kinase assay evidence +def: "A type of enzymatic activity assay evidence that measures transfer of a phosphate to a peptide or protein substrate by a protein kinase." [url:https\://www.ncbi.nlm.nih.gov/books/NBK91991/] +is_a: ECO:0000005 ! enzymatic activity assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: jbmunro +created_by: mchibucos +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001559 +name: sumoylation assay evidence +def: "A type of direct assay evidence where sumoylated residues on a protein are detected." [SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:000156 +name: single-cell RNA-sequencing evidence used in automatic assertion +def: "A type of single cell RNA-sequencing evidence that is used in automatic assertion." [] +is_a: ECO:0001560 ! single-cell RNA-sequencing evidence +is_a: ECO:0006069 ! RNA-sequencing evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: jbmunro +creation_date: 2019-01-02T12:00:00Z + +[Term] +id: ECO:0001560 +name: single-cell RNA-sequencing evidence +def: "A type of RNA-sequencing evidence that uses a single cell as the source of the RNA." [] +synonym: "scRNA-seq evidence" EXACT [] +is_a: ECO:0000295 ! RNA-sequencing evidence +property_value: IAO:0000234 "Bgee" xsd:string +created_by: jbmunro +creation_date: 2019-01-02T12:00:00Z + +[Term] +id: ECO:0001561 +name: translation assay evidence +def: "A type of direct assay evidence where de novo protein synthesis is detected." [PMID:18230759, PMID:24901308, SIB:PG] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001562 +name: ubiquitination assay evidence +def: "A type of direct assay evidence where ubiquitinated residues on a protein are detected." [PMID:19692941, SIB:PG] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001563 +name: cell growth assay evidence +def: "A type of direct assay evidence where biological cell development, i.e. an increase in cell mass and size are measured." [GO:0016049, PMID:11057898] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000112 "However, as shown in Fig. 5A, the recombinant mycobacterial cells became sensitive to the anti-TB drugs isoniazid and streptomycin, as evidenced by their inhibited growth in the presence of 25 mug/mL of isoniazid or 0.5 mug/mL of streptomycin in the medium." xsd:string {xref="PMID:20843371"} +property_value: IAO:0000112 "Similar growth profiles of these strains were observed in liquid media with the corresponding inducers (data not shown). These indicate that the expression of vapC10 alone led to growth arrest of E. coli, and the simultaneous expression of vapB10 could neutralize this growth-inhibition effect, suggesting that vapC10 encodes a TA toxin and vapB10 encodes the cognate antitoxin." xsd:string {xref="PMID:24260461"} +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001565 +name: cell-based assay evidence +def: "A type of direct assay evidence resulting from the study of cells." [] +is_a: ECO:0000002 ! direct assay evidence +created_by: jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001566 +name: quantitative reverse transcription polymerase chain reaction evidence +def: "A type of reverse transcription polymerase chain reaction evidence that combines reverse transcription polymerase chain reaction and real time polymerase chain reaction to quantitatively assay for the detection of RNA levels." [] +synonym: "qRT-PCR evidence" EXACT [] +synonym: "quantitative real-time RT-PCR evidence" EXACT [] +synonym: "quantitative RT-PCR evidence" EXACT [] +synonym: "real-time qRT-PCR evidence" EXACT [] +synonym: "real-time quantitative reverse transcription PCR evidence" EXACT [] +synonym: "real-time reverse transcription polymerase chain reaction evidence" EXACT [] +synonym: "real-time RT-PCR evidence" EXACT [] +synonym: "RRT-PCR evidence" EXACT [] +synonym: "rRT-PCR evidence" EXACT [] +synonym: "RT-qPCR evidence" EXACT [] +is_a: ECO:0000109 ! reverse transcription polymerase chain reaction evidence +property_value: IAO:0000112 "The real-time RT-PCR validated that Zur repressed the first gene of each of the three operons, znuA, znuCB and ykgM-rpmJ2 (Additional file 5)." xsd:string {xref="PMID:19552825"} +created_by: jbmunro +creation_date: 2018-07-07T12:00:00Z + +[Term] +id: ECO:0001567 +name: quantitative reverse transcription polymerase chain reaction evidence used in manual assertion +def: "A type of quantitative reverse transcription polymerase chain reaction evidence used in manual assertion." [] +is_a: ECO:0001566 ! quantitative reverse transcription polymerase chain reaction evidence +is_a: ECO:0001808 ! reverse transcription polymerase chain reaction evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: jbmunro +creation_date: 2018-07-07T12:00:00Z + +[Term] +id: ECO:0001568 +name: quantitative reverse transcription polymerase chain reaction evidence used in automatic assertion. +alt_id: ECO:000156 +def: "A type of quantitative reverse transcription polymerase chain reaction evidence used in automatic assertion." [] +is_a: ECO:0001566 ! quantitative reverse transcription polymerase chain reaction evidence +is_a: ECO:0007475 ! reverse transcription polymerase chain reaction evidence used in automatic assertion +created_by: jbmunro +creation_date: 2018-07-07T12:00:00Z + +[Term] +id: ECO:000157 +name: colony diameter phenotype evidence used in manual assertion +def: "A type of colony diameter phenotype evidence that is used in manual assertion." [] +is_a: ECO:0001571 ! colony diameter phenotype evidence +is_a: ECO:0007082 ! colony size phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: jbmunro +creation_date: 2019-01-03T12:00:00Z + +[Term] +id: ECO:0001570 +name: single-cell RNA-sequencing evidence used in manual assertion +def: "A type of single cell RNA-sequencing evidence that is used in manual assertion." [] +is_a: ECO:0001560 ! single-cell RNA-sequencing evidence +is_a: ECO:0006068 ! RNA-sequencing evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: jbmunro +creation_date: 2019-01-02T12:00:00Z + +[Term] +id: ECO:0001571 +name: colony diameter phenotype evidence +def: "A type of colony size phenotypic evidence resulting from measurement of a microbial colony's diameter." [] +is_a: ECO:0007081 ! colony size phenotypic evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-01-03T12:00:00Z + +[Term] +id: ECO:0001572 +name: colony diameter phenotype evidence used in automatic assertion +def: "A type of colony diameter phenotype evidence that is used in automatic assertion" [] +is_a: ECO:0001571 ! colony diameter phenotype evidence +is_a: ECO:0007627 ! colony size phenotypic evidence used in automatic assertion +created_by: jbmunro +creation_date: 2019-01-03T12:00:00Z + +[Term] +id: ECO:0001574 +name: membrane fusion assay evidence +def: "A type of direct assay evidence in which a broad variety of physiological processes are measured when two separate membranes merge into a single contiguous membrane." [PMID:1836993] +comment: Measurements can include but are not limited to: synaptic transmission, fertilization, and viral entry. +is_a: ECO:0000002 ! direct assay evidence +created_by: jbmunro +creation_date: 2019-03-01T12:00:00Z + +[Term] +id: ECO:0001575 +name: membrane fusion assay evidence used in automatic assertion +def: "A type of membrane fusion assay evidence that is used in automatic assertion." [] +is_a: ECO:0001574 ! membrane fusion assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: jbmunro +creation_date: 2019-03-01T12:00:00Z + +[Term] +id: ECO:0001576 +name: membrane fusion assay evidence used in manual assertion +def: "A type of membrane fusion assay evidence that is used in manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001574 ! membrane fusion assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: jbmunro +creation_date: 2019-03-01T12:00:00Z + +[Term] +id: ECO:0001577 +name: spheroplast fusion assay evidence +def: "A type of membrane fusion assay evidence based on the fusion of spheroplasts." [] +comment: A spheroplast is a Gram-negative bacterium cell in which the cell wall has been almost completely removed. {xref="PMID:25870259"} +is_a: ECO:0001574 ! membrane fusion assay evidence +created_by: jbmunro +creation_date: 2019-03-01T12:00:00Z + +[Term] +id: ECO:0001578 +name: spheroplast fusion assay evidence used in automatic assertion +def: "A type of spheroplast fusion assay evidence used in automatic assertion." [] +is_a: ECO:0001575 ! membrane fusion assay evidence used in automatic assertion +is_a: ECO:0001577 ! spheroplast fusion assay evidence +created_by: jbmunro +creation_date: 2019-03-01T12:00:00Z + +[Term] +id: ECO:0001579 +name: spheroplast fusion assay evidence used in manual assertion +def: "A type of spheroplast fusion assay evidence that is used in manual assertion." [] +is_a: ECO:0001576 ! membrane fusion assay evidence used in manual assertion +is_a: ECO:0001577 ! spheroplast fusion assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: jbmunro +creation_date: 2019-03-01T12:00:00Z + +[Term] +id: ECO:0001580 +name: liquid chromatography coupled with tandem mass spectrometry evidence +def: "A type of mass spectrometry evidence where liquid chromatography is used to separate particles in solution, followed by fragmentation and measurement of the mass-to-charge ratio of the resulting particles to identify the amount and type of material entities present in a sample." [] +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-03-26T12:00:00Z + +[Term] +id: ECO:0001581 +name: liquid chromatography coupled with tandem mass spectrometry evidence used in automatic assertion +def: "A type of liquid chromatography coupled with tandem mass spectrometry evidence that is used in an automatic assertion." [] +is_a: ECO:0001580 ! liquid chromatography coupled with tandem mass spectrometry evidence +is_a: ECO:0007433 ! mass spectrometry evidence used in automatic assertion +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-03-26T12:00:00Z + +[Term] +id: ECO:0001582 +name: liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion +def: "A type of liquid chromatography coupled with tandem mass spectrometry evidence that is used in a manual assertion." [] +is_a: ECO:0001230 ! mass spectrometry evidence used in manual assertion +is_a: ECO:0001580 ! liquid chromatography coupled with tandem mass spectrometry evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-03-26T12:00:00Z + +[Term] +id: ECO:0001583 +name: small interfering RNA knockdown evidence +def: "A type of anti-sense experiment evidence where gene expression is disrupted through the introduction of double-stranded RNA molecules, 20-25 base pairs in length, which operate within the RNA interference pathway." [] +synonym: "short interfering RNA" EXACT [] +synonym: "silencing RNA" EXACT [] +synonym: "siRNA" EXACT [] +is_a: ECO:0000018 ! anti-sense experiment evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-03T12:00:00Z + +[Term] +id: ECO:0001584 +name: small interfering RNA knockdown evidence used in automatic assertion +def: "A type of small interfering RNA knockdown evidence that is used in an automatic assertion." [] +is_a: ECO:0001583 ! small interfering RNA knockdown evidence +is_a: ECO:0007556 ! anti-sense experiment evidence used in automatic assertion +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-03T12:00:00Z + +[Term] +id: ECO:0001585 +name: small interfering RNA knockdown evidence used in manual assertion +def: "A type of small interfering RNA knockdown evidence that is used in a manual assertion." [] +is_a: ECO:0001583 ! small interfering RNA knockdown evidence +is_a: ECO:0006050 ! anti-sense experiment evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-03T12:00:00Z + +[Term] +id: ECO:0001586 +name: ion trap mass spectrometry evidence +def: "A type of mass spectrometry evidence where a combination of magnetic and/or electric fields are utilized to capture charged particles in tandem with mass spectrometry." [PMID:1561330] +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001587 +name: ion trap mass spectrometry evidence used in automatic assertion +def: "A type of ion trap mass spectrometry evidence that is used in an automatic assertion." [] +is_a: ECO:0001586 ! ion trap mass spectrometry evidence +is_a: ECO:0007433 ! mass spectrometry evidence used in automatic assertion +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001588 +name: ion trap mass spectrometry evidence used in manual assertion +def: "A type of ion trap mass spectrometry evidence that is used in a manual assertion." [] +is_a: ECO:0001230 ! mass spectrometry evidence used in manual assertion +is_a: ECO:0001586 ! ion trap mass spectrometry evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001589 +name: atomic force microscopy evidence +def: "A type of microscopy evidence where a mechanical probe is applied to a sample (i.e. a type of scanning probe microscopy) which can result in production of an image (with resolution in the order of fractions of a nanometer), force measurement, or sample manipulation." [] +comment: Atomic force microscopy can be used for force measurement (force spectroscopy), imaging (rendering a three-dimensional shape (topography), and manipulation of the sample. +synonym: "AFM" EXACT [] +synonym: "scanning force microscopy" EXACT [] +synonym: "SFM" EXACT [] +is_a: ECO:0001098 ! microscopy evidence +property_value: IAO:0000112 "AFM revealed loop structures stabilized by multiple EspR dimer of dimers suggesting the presence of several distant EspR binding sites in the espACD upstream region." xsd:string {xref="PMID:22479184"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001590 +name: atomic force microscopy evidence used in automatic assertion +def: "A type of atomic force microscopy evidence that is used in an automatic assertion." [] +is_a: ECO:0001589 ! atomic force microscopy evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001591 +name: atomic force microscopy evidence used in manual assertion +def: "A type of atomic force microscopy evidence that is used in a manual assertion." [] +is_a: ECO:0001589 ! atomic force microscopy evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001592 +name: polysome profiling evidence +def: "A type of experimental evidence employed to study the associations between multiple ribosomes and mRNA that utilizes density gradient centrifugation to separate out the lysate of cells of interest with the objective of isolating polysomes/polyribosomes which are complexes of an mRNA molecule and two or more ribosomes that act to translate mRNA. The optical density of the fractions is then determined." [PMID:30545912] +comment: Once isolated, gel electrophoresis, immunoblot, and other protein profiling techniques can be applied to garner more information. +is_a: ECO:0000006 ! experimental evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001593 +name: polysome profiling evidence used in automatic assertion +def: "A type of polysome profiling evidence that is used in an automatic assertion." [] +is_a: ECO:0001592 ! polysome profiling evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001594 +name: polysome profiling evidence used in manual assertion +def: "A type of polysome profiling evidence that is used in a manual assertion." [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001592 ! polysome profiling evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001598 +name: multilocus sequence typing evidence +def: "A type of nucleotide sequencing assay evidence in which DNA sequence variations within a specific set of genes (typically housekeeping genes) are used to characterizes strains by their unique allelic profiles." [PMID:9501229] +comment: See PMID:24713082 for use example. +synonym: "MLST" EXACT [] +is_a: ECO:0000219 ! nucleotide sequencing assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001599 +name: multilocus sequence typing evidence used in automatic assertion +def: "A type of multilocus sequence typing evidence that is used in an automatic assertion." [] +is_a: ECO:0001598 ! multilocus sequence typing evidence +is_a: ECO:0007813 ! nucleotide sequencing assay evidence used in automatic assertion +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001600 +name: multilocus sequence typing evidence used in manual assertion +def: "A type of multilocus sequence typing evidence that is used in a manual assertion" [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001598 ! multilocus sequence typing evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001601 +name: protein-oligonucleotide microarray binding evidence +def: "A type of protein-binding evidence that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites." [PMID:22146299] +synonym: "PBM evidence" EXACT [] +is_a: ECO:0000024 ! protein binding evidence +is_a: ECO:0000136 ! nucleic acid binding evidence +created_by: swolfish +creation_date: 2015-06-05T14:36:00Z + +[Term] +id: ECO:0001603 +name: cell staining evidence +def: "A type of cell-based assay evidence in which living cells are stained with a vital stain, i.e. dye that can be used on living cells without causing cell death." [GOC:MAH] +synonym: "vital staining evidence" RELATED [GOC:MAH] +is_a: ECO:0001565 ! cell-based assay evidence +created_by: swolfish +creation_date: 2015-06-08T11:51:37Z + +[Term] +id: ECO:0001801 +name: alkaline phosphatase reporter gene assay evidence +def: "A type of reporter gene assay evidence based on the fusion of selected genes with the phoA gene to express alkaline phosphatase in periplasmic space for protein tracing." [ECO:SW, PMID:11823238] +comment: Alkaline phosphatase reporter assay produces a hybrid protein with alkaline phosphatase activity following transportation across the cellular membrane. +synonym: "SEAP reporter assay" RELATED [] +synonym: "Secreted Embryonic Alkaline Phosphatase reporter assay" RELATED [] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-10-29T15:53:45Z + +[Term] +id: ECO:0001802 +name: beta-galactosidase reporter gene assay evidence +alt_id: ECO:0000297 +def: "A type of reporter gene assay evidence based on the fusion of the lacZ gene to a specific promoter for the expression of beta-galactosidase which will appear blue when grown on a X-gal medium." [ECO:SW, PMID:20439410] +comment: The assay is often performed using a plasmid borne construction on a lacZ strain. +synonym: "beta-gal reporter gene assay" EXACT [] +synonym: "beta-galactosidase assay evidence" EXACT [] +synonym: "lacZ reporter gene assay" RELATED [] +synonym: "LacZ transcript localization evidence" EXACT [] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000112 "For each of the three genes, there was a significant increase of beta-galactosidase activity in Deltazur compared to WT when they grew in TMH with the addition of zinc. Thus, Zur repressed the promoter activities of znuC, znuA and ykgM." xsd:string {xref="PMID:19552825"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-10-29T15:57:10Z + +[Term] +id: ECO:0001803 +name: chloramphenicol acetyltransferase reporter gene assay evidence +def: "A type of reporter gene assay evidence based on the fusion of the CAT gene to a specific promoter for the expression of chloramphenicol acetyltransferase which confers resistance to the chloramphenicol antibiotic." [ECO:SW, PMID:1630936] +comment: The amount of acetylated chloramphenicol is directly proportional to the amount of CAT enzyme present. +synonym: "CAT reporter gene assay" EXACT [PMID:1630936] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-10-29T16:06:42Z + +[Term] +id: ECO:0001804 +name: beta-glucuronidase reporter gene assay evidence +def: "A type of reporter gene assay evidence where the beta-glucuronidase enzyme from Escherichia coli is used as the reporter to transform non-fluorescent substrates into fluorescents for detection." [ECO:SW] +synonym: "GUS reporter gene assay" EXACT [] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-10-29T16:11:02Z + +[Term] +id: ECO:0001805 +name: luciferase reporter gene assay evidence +def: "A type of reporter gene assay evidence where luciferase, an oxidative enzyme, is used as the reporter to detect a gene product with bioluminescence." [ECO:SW, PMID:17623934] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-10-29T16:15:39Z + +[Term] +id: ECO:0001806 +name: chromatin immunoprecipitation- exonuclease evidence +def: "A type of chromatin immunoprecipitation evidence that uses gama-exonuclease to digest TF-unbound DNA after ChIP for the identification of transcription factor binding site locations with high-resolution data." [ECO:SW, PMID:25249628] +synonym: "ChIP-exo" EXACT [PMID:25249628] +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-10-29T16:37:36Z + +[Term] +id: ECO:0001807 +name: electrophoretic mobility shift assay evidence used in manual assertion +def: "A type of electrophoretic mobility shift that is used in a manual assertion." [ECO:SW] +synonym: "electrophoretic mobility shift assay" BROAD [] +synonym: "EMSA evidence" BROAD [] +synonym: "gel retardation assay" RELATED [] +is_a: ECO:0000096 ! electrophoretic mobility shift assay evidence +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: swolfish +creation_date: 2015-11-03T12:13:17Z + +[Term] +id: ECO:0001808 +name: reverse transcription polymerase chain reaction evidence used in manual assertion +def: "A type of reverse transcription polymerase chain reaction evidence that is used in a manual assertion." [ECO:SW] +is_a: ECO:0000109 ! reverse transcription polymerase chain reaction evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: swolfish +creation_date: 2015-11-03T12:22:46Z + +[Term] +id: ECO:0001809 +name: DNA affinity chromatography evidence +def: "A type of chromatography evidence where immobilized promoter DNA is labeled and bound to a matrix through which a protein mixture is poured and then eluted to purify specific DNA binding proteins." [ECO:SW, PMID:11694305, PMID:11725488] +comment: Biotinylation is commonly used for labeling and beads are commonly used as the matrix. Bound protein will remain attached to the beads. Results can be detected through gel electrophoresis and then sequenced by mass-spectrometry. This technique can be used to demonstrate binding of a purified protein, or to purify the binding protein from crude extract or protein mixture. +synonym: "DNA affinity purification" RELATED [] +is_a: ECO:0000325 ! chromatography evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-03T13:48:49Z + +[Term] +id: ECO:0001810 +name: DNAse footprinting evidence +def: "A type of nucleic acid binding evidence where proteins that have been bound to DNA protect a binding site from enzymatic cleavage with DNAse, thereby detecting protein-DNA interactions." [ECO:SW, PMID:212715, PMID:22194258] +comment: In DNAse footingprinting amplified DNA combined with TF and DNAse is electrophoresed for fragment comparison with a non-TF control sample. TF-bound fragments do not appear in the gel. Such fragments can be isolated, purified, and sequenced. This technique is often used to identify the binding motif of a TF resolving the protected region to 50-100 bp which can be further examined for TF-binding sites. +synonym: "DNase protection" RELATED [] +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000112 "Subsequent DNase I footprinting experiments (Fig. 5d) indicated that His-PhoP protected a single region located from 102 to 47 bp upstream of rovA. This footprint was considered the PhoP site." xsd:string {xref="PMID:21966533"} +property_value: IAO:0000112 "The subsequent DNase I footprinting experiments (Figure 3a) showed that His-OmpR-P protected a single region within the ompR promoter. Therefore, OmpR stimulated its own gene at the transcriptional level, which was mediated through the binding of OmpR-P to its own promoter." xsd:string {xref="PMID:21345178"} +property_value: IAO:0000112 "To precisely determine the PhoP-binding sites of target genes, DNase I footprinting assay was performed on 17 PhoP-dependent promoter DNA regions (both coding and noncoding strands) (Additional file 6). DNase I footprinting results confirmed the direct binding of His-PhoP to these promoter regions in vitro." xsd:string {xref="PMID:18366809"} +property_value: IAO:0000112 "We therefore pursued DNase I footprinting assays on several of the Crp-associated sequences that gave an unusual downshift in our initial EMSA trials, in an effort to identify a consensus binding sequence (see Fig. S2 in the supplemental material). We mapped sites upstream of crp itself, SCO4561 and SCO2977, and identified a consensus binding sequence [GTG(N)6GNCAC]; derivatives of this motif could be found in all of the secondary metabolism-associated target sequences, although notably, one-half of the palindrome seemed to be better conserved than the other [GTG(N)6GNGAN] (Fig. 2C; Table 1)." xsd:string {xref="PMID:23232715"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-03T13:55:15Z + +[Term] +id: ECO:0001811 +name: fluorescence anisotropy evidence +def: "A type of fluorescence evidence based on rapid and quantitative analysis of diverse molecular interactions, enzyme activities, and nucleic acid hybridization which uses a fluorophore to measure the binding constants and kinetics of reactions that cause a change in the rotational time of the molecules." [ECO:SW, PMID:20232898] +comment: The degree of polarization of a fluorophore is inversely related to its molecular rotation. When the fluorophore is bound to a small molecule, the rate at which it tumbles can decrease significantly from when it is bound tightly to a large protein. If the fluorophore is attached to the larger protein in a binding pair, the difference in polarization between bound and unbound states will be smaller and less accurate. The degree of binding is calculated by using the difference in anisotropy of the partially bound, free, and fully bound states measured by titrating the two binding partners. Fluorescence polarixation assays are homogeneous. +synonym: "FA" EXACT [] +synonym: "Fluorescence polarization" RELATED [PMCID:3277431, PMID:20232898] +synonym: "FP" RELATED [PMCID:3277431, PMID:20232898] +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000112 "Since these FA analyses are conducted with 300 nM scOhrR, this suggests that oxidation leads to at least a 100-fold reduction in DNA-binding affinity." xsd:string {xref="PMID:19129220"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-03T14:01:36Z + +[Term] +id: ECO:0001812 +name: genomic systematic evolution of ligands by exponential amplification evidence +def: "A type of systematic evolution of ligands by exponential amplification evidence based on a restricted genomic DNA library to identify naturally occurring genomic aptamers and RNA-protein interaction networks with RNA-binding protein as bait and high-throughput sequencing." [ECO:SW, PMID:20541015, PMID:21720957] +comment: The reported sequences should always be verified for presence in the genome sequence. +synonym: "genomic SELEX" EXACT [PMID:20541015] +is_a: ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-03T14:05:37Z + +[Term] +id: ECO:0001813 +name: heteronuclear single quantum coherence spectroscopy evidence +def: "A type of nuclear magnetic resonance spectroscopy evidence based on two-dimensional NMR for elucidation of the chemical structure of an isolated or synthesized chemical compound where the characteristic transfer magnetization of a proton to a nitrogen or carbon isotope is monitored by NMR, generating a specific peak in the spectrum." [ECO:SW, PMID:19856946] +comment: Chemical shifts in the spectrum indicating binding can be detected from analysis of a protein spectrum in the presence or absence of its cognate DNA binding site. +synonym: "HSQC" EXACT [PMID:19856946] +is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-03T14:11:06Z + +[Term] +id: ECO:0001814 +name: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence +def: "A type of nucleic acid binding evidence where the synthetic molecule methidiumpropyl-EDTA (MPE) is used to cleave ligand-protected DNA, followed by analysis of the restriction fragments to generate a footprint (i.e. size and location) of small molecule binding sites on the DNA." [ECO:SW, PMID:6225070] +synonym: "Methidiumpropyl-EDTA Fe(II) footprinting" RELATED [PMID:6225070] +synonym: "MPE Fe(II) footprinting" RELATED [PMID:6225070] +synonym: "MPE-EDTA Fe(II) footprinting" RELATED [PMID:6225070] +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-03T14:24:04Z + +[Term] +id: ECO:0001815 +name: copper-phenanthroline footprinting evidence +def: "A type of nucleic acid binding evidence where nucleic acid that has been bound to protein is cleaved with 1,10-phenanthroline-copper complex resulting in a high-resolution footprint of sequence-specific protein-DNA contacts." [ECO:SW, PMID:11691942] +synonym: "1,10-Phenanthroline-copper footprinting" EXACT [PMID:1384472] +synonym: "OP-Cu Complex" RELATED [PMID:1384472] +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T08:37:51Z + +[Term] +id: ECO:0001816 +name: green fluorescent protein reporter gene assay evidence +alt_id: ECO:0000296 +def: "A type of reporter gene assay evidence based on the fusion of select genes with the green fluorescent protein (GFP) gene for detection with bioluminescence of the gene product when exposed to blue ultraviolet light." [ECO:SW, PMID:11989662] +synonym: "GFP promoter fusion" RELATED [] +synonym: "GFP reporter gene assay" EXACT [PMID:11989662] +synonym: "green fluorescent protein promoter fusion" RELATED [] +synonym: "green fluorescent protein transcript localization evidence" EXACT [] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T08:58:42Z + +[Term] +id: ECO:0001817 +name: glutathione S-transferase pull-down assay evidence +def: "A type of bait-prey hybrid interaction evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions." [ECO:SW, PMID:26096507] +comment: The recombinant protein can also be eluted following the pull-down and analyzed further with qPCR or sequencing. +synonym: "GST pull-down assay" EXACT [PMID:26096507] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T09:05:56Z + +[Term] +id: ECO:0001818 +name: hydroxyl-radical footprinting evidence +def: "A type of nucleic acid binding evidence used to identify protein-binding sites on the DNA molecule where DNA that has been bound to protein is digested with hydroxyl radical produced by reduction of hydrogen peroxide with iron (II), followed by separating the cleavage products on a denaturing electrophoresis gel." [ECO:SW, PMID:18546600, PMID:19378159, PMID:3090544] +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T09:20:26Z + +[Term] +id: ECO:0001819 +name: primer extension assay evidence +def: "A type of transcript expression evidence used to determine expression levels of mRNA where a labeled synthetic oligonucleotide primer is annealed to mRNA downstream of the presumed transcription start site of a gene. The mRNA is extended with reverse transcriptase, producing cDNA which is electrophoresed, and the band size is enables determination of the 5' end of the mRNA (i.e. the transcription start site)." [ECO:SW, PMID:23378648] +comment: The most commonly used radiolabel is 32P. +is_a: ECO:0000009 ! transcript expression evidence +property_value: IAO:0000112 "The primer extension assay (Fig. 6a) defined the transcription start sites the three sRNA genes qrr2 - 4, and this assay also indicated that the promoter activity of all the thee qrr genes was under the positive control of OpaR." xsd:string {xref="PMID:22506036"} +property_value: IAO:0000112 "The primer extension assay detected two transcriptional start sites located at 343 and 78 bp upstream of rovA (Fig. 2); therefore, two promoters (named P2 and P1, respectively) were transcribed for rovA." xsd:string {xref="PMID:21966533"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T09:33:18Z + +[Term] +id: ECO:0001820 +name: rapid amplification of cDNA ends polymerase chain reaction evidence +def: "A type of nucleotide sequencing assay evidence in which RT-PCR (cDNA synthesis) is first used to produce a cDNA copy of a region of the RNA transcript being investigated followed by PCR to capture either the unknown 5' or 3' end of the transcript for sequencing, depending on whether 5' RACE-PCR or 3' RACE-PCR are being undertaken." [ECO:SW, PMID:17498297, PMID:7685466] +comment: 5' and 3' RACE-PCR utilize different protocols to amplify an unknown end using the known sequence of the center of the transcript. For cDNA synthesis, 5' RACE-PCR uses an anti-sense oligonucleotide primer (gene specific primer (GSP)) that recognizes a known sequence in the middle of the gene of interest and the reverse transcriptase to add base pairs to the 3' end of the primer. Next, terminal deoxynucleotidyl transferase (TdT) is used to add a string of identical nucleotides to the 3' end of the cDNA. A PCR reaction is then carried out, using a second anti-sense gene specific primer (GSP2) that binds to the known sequence, and a sense (forward) universal primer (UP) that binds the homopolymeric tail added to the 3' ends of the cDNAs to amplify a cDNA product from the 5' end. In contrast, 3' RACE-PCR utilizes the naturally occurring 3' polyA tail of the transcript and uses an Oligo-dT-adaptor primer (a primer with a short sequence of deoxy-thymine nucleotides) that complements the polyA tail and adds a special adaptor sequence to the 5' end of each cDNA. PCR is then used to amplify 3' cDNA from a known region using a sense GSP, and an anti-sense primer complementary to the adaptor sequence. +comment: RACE PCR is frequently used to verify transcription start sites relevant to the function of transcription factor binding sites such as repression. +synonym: "3’ RACE-PCR" EXACT [] +synonym: "5’ RACE-PCR" EXACT [] +synonym: "RACE PCR" EXACT [PMI:17498297] +is_a: ECO:0000219 ! nucleotide sequencing assay evidence +property_value: IAO:0000112 "5' RACE analysis was employed to localize the espR promoter using RNA extracted from Mtb H37Rv grown to mid-log phase. The espR transcript starts with a poly-G (7) sequence 144 bp upstream of the translational start codon." xsd:string {xref="PMID:22479184"} +property_value: IAO:0000112 "The 5'-end of the espA mRNA was located 66 bp upstream of the translation start codon using 5' RACE (Fig. S3)." xsd:string {xref="PMID:22479184"} +property_value: IAO:0000112 "Using 5' rapid amplification of cDNA ends (RACE), we mapped the transcriptional start sites of rpoQ and VF_A1016 (Fig. 4A). Neither start site was detected in the DeltarpoQ mutant, supporting the idea that transcription of rpoQ and VF_A1016 requires RpoQ (data not shown)." xsd:string {xref="PMID:22233679"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T09:48:38Z + +[Term] +id: ECO:0001821 +name: RNA sequencing assay evidence +comment: Use ECO:0000295 (RNA-seq evidence) in place of this term. +property_value: IAO:0000234 "CollecTF" xsd:string +is_obsolete: true +created_by: swolfish +creation_date: 2015-11-04T10:15:57Z + +[Term] +id: ECO:0001822 +name: survival rate analysis evidence +def: "A type of knockout evidence based on the survival of an organism in a particular environment where a gene for an enzyme or regulator is knocked out and results are used as a natural reporter." [ECO:SW] +is_a: ECO:0001091 ! knockout phenotypic evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T10:48:59Z + +[Term] +id: ECO:0001823 +name: x-ray crystallography evidence +def: "A type of crystallography evidence where a purified sample at high concentration is crystallised and the crystals are exposed to an x ray beam to obtain three dimensional molecular structure of proteins and biological macromolecules." [ECO:SW, PMID:23135450, PMID:24648090] +comment: X-ray crystallography can be used for a crystal used to probe at the tridimensional structure of proteins. In some cases, it is possible to co-crystallize the protein bound to its DNA binding site, providing detail on the particular arrangement of the two components. +is_a: ECO:0001034 ! crystallography evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T10:56:22Z + +[Term] +id: ECO:0001824 +name: DNA adenine methyltransferase identification evidence +def: "A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site." [ECO:SW, PMID:16938559, PMID:17545983, PMID:19588092] +comment: The methylation tag is detected with methylation-sensitive restriction enzymes, DpnI and DpnII. +synonym: "DamID" EXACT [PMID:19588092] +is_a: ECO:0000023 ! affinity evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-05T15:56:13Z + +[Term] +id: ECO:0001825 +name: isothermal titration calorimetry evidence +def: "A type of affinity evidence where the absorbed or released heat of a biomolecular binding event is directly measured in a reference cell and sample cell after the addition of a ligand using a microcalorimeter for a complete thermodynamic profile of the molecular interaction." [ECO:SW, PMID:10527727] +comment: When ITC is used to study TF, the temperature of two identical cells containing a known concentration of TF is monitored. After the ligand is added to the cells in precisely measured aliquots, the temperature difference in each cell is observed to determine target binding. This value can be used to compute the energetics of the reaction and hence, binding affinity of the TF for the DNA fragment. The thermodynamic profile that is measured includes the values of binding constant (K(a)), stoichiometry (n), and the enthalpy of binding (DeltaH(b)). +synonym: "ITC" EXACT [PMID:10527727] +is_a: ECO:0000023 ! affinity evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-05T16:06:20Z + +[Term] +id: ECO:0001826 +name: ultraviolet light footprinting evidence +def: "A type of nucleic acid binding evidence where DNA is non-specifically fragmented with ultraviolet light while protein-bound regions are protected from UV damage and strand breakage patterns are analyzed by PAGE and sequenced to detect protein-DNA contacts." [ECO:SW, PMID:2842760, PMID:7602584] +synonym: "ultraviolet footprinting" RELATED [] +synonym: "UV footprinting" RELATED [] +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-05T16:13:36Z + +[Term] +id: ECO:0001827 +name: methylation interference footprinting evidence +def: "A type of nucleic acid binding evidence used to identify protein binding sites on the DNA molecule where multiple copies of a DNA fragment containing a putative TF-binding site are randomly methylated with dimethyl sulfate (DMS) and cleaved at the methyl group, followed by separating the cleavage products on a denaturing electrophoresis gel." [ECO:SW, PMID:1583685] +synonym: "methylation interference footprinting evidence" EXACT [] +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-05T16:16:27Z + +[Term] +id: ECO:0001828 +name: inference of sequence features from visual inspection used in manual assertion +def: "A type of inference of sequence features from visual inspection that is used in a manual assertion." [ECO:RCJ] +synonym: "visual sequence inspection evidence" BROAD [] +is_a: ECO:0007751 ! inferential evidence used in manual assertion +is_a: ECO:0007850 ! inference of sequence features from visual inspection +created_by: swolfish +creation_date: 2015-11-10T14:40:16Z + +[Term] +id: ECO:0001829 +name: ferric uptake regulator titration assay evidence +def: "A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis." [ECO:SW, PMID:10713425, PMID:7642488, PMID:8107138] +synonym: "Fluorescence polarization" RELATED [PMID:15689115] +synonym: "FURTA" EXACT [PMID:10713425] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-10T14:55:36Z + +[Term] +id: ECO:0001830 +name: host colonization assay evidence +def: "A type of experimental evidence in which the colonization or adhesion capacity is measured." [] +comment: Has to do with the ability of the bacterium or virus to bind to a host. +synonym: "adhesion assay evidence" EXACT [] +is_a: ECO:0000006 ! experimental evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001831 +name: host colonization assay evidence used in automatic assertion +def: "A type of host colonization assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001830 ! host colonization assay evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001832 +name: host colonization assay evidence used in manual assertion +def: "A type of host colonization assay evidence that is used in a manual assertion." [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001830 ! host colonization assay evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001833 +name: infection assay evidence +def: "A type of host colonization assay evidence in which the infection capacity is measured by assessing changes in the host." [] +comment: Has to do with the outcome of a bacterial or viral / host interaction, be it a measure of cell death, cytotoxicity, invasion, etc. +is_a: ECO:0001830 ! host colonization assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001834 +name: infection assay evidence used in automatic assertion +def: "A type of infection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001831 ! host colonization assay evidence used in automatic assertion +is_a: ECO:0001833 ! infection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001835 +name: infection assay evidence used in manual assertion +def: "A type of infection assay evidence that is used in a manual assertion." [] +is_a: ECO:0001832 ! host colonization assay evidence used in manual assertion +is_a: ECO:0001833 ! infection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001836 +name: in situ hybridization evidence +def: "A type of expression pattern evidence in which single-stranded DNA or RNA (probe) are annealed to complementary DNA or RNA in a portion or section of tissue." [] +is_a: ECO:0000008 ! expression pattern evidence +property_value: IAO:0000234 "planarian anatomy ontology (PLANA)" xsd:string +created_by: jbmunro +creation_date: 2019-09-26T12:00:00Z + +[Term] +id: ECO:0001837 +name: in situ hybridization evidence used in automatic assertion +def: "A type of in situ hybridization evidence that is used in an automatic assertion." [] +is_a: ECO:0001836 ! in situ hybridization evidence +is_a: ECO:0007298 ! expression pattern evidence used in automatic assertion +created_by: jbmunro +creation_date: 2019-09-26T12:00:00Z + +[Term] +id: ECO:0001838 +name: in situ hybridization evidence used in manual assertion +def: "A type of in situ hybridization evidence that is used in a manual assertion." [] +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0001836 ! in situ hybridization evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: jbmunro +creation_date: 2019-09-26T12:00:00Z + +[Term] +id: ECO:0001839 +name: colorimetric in situ hybridization evidence +def: "A type of nucleic acid localization evidence and in situ hybridization evidence resulting from the use of colorimetric probes to detect complementary sequences of nucleic acids." [PMID:23497040] +is_a: ECO:0001836 ! in situ hybridization evidence +property_value: IAO:0000234 "planarian anatomy ontology (PLANA)" xsd:string +created_by: jbmunro +creation_date: 2019-09-26T12:00:00Z + +[Term] +id: ECO:0001840 +name: colorimetric in situ hybridization evidence used in automatic assertion +def: "A type of colorimetric in situ hybridization evidence that is used in an automatic assertion." [] +is_a: ECO:0001837 ! in situ hybridization evidence used in automatic assertion +is_a: ECO:0001839 ! colorimetric in situ hybridization evidence +created_by: jbmunro +creation_date: 2019-09-26T12:00:00Z + +[Term] +id: ECO:0001841 +name: colorimetric in situ hybridization evidence used in manual assertion +def: "A type of colorimetric in situ hybridization evidence that is used in a manual assertion." [] +is_a: ECO:0001838 ! in situ hybridization evidence used in manual assertion +is_a: ECO:0001839 ! colorimetric in situ hybridization evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: jbmunro +creation_date: 2019-09-26T12:00:00Z + +[Term] +id: ECO:0001842 +name: random mutagenesis of specific target DNA evidence +def: "A type of random mutagenesis phenotypic evidence resulting from the random mutation of a specifically targeted region of DNA." [] +is_a: ECO:0005529 ! random mutagenesis phenotypic evidence +property_value: IAO:0000234 "ECO" xsd:string +created_by: jbmunro +creation_date: 2019-12-09T12:00:00Z + +[Term] +id: ECO:0001843 +name: random mutagenesis of specific target DNA evidence used in automatic assertion +def: "A type of random mutagenesis of specific target DNA evidence that is used in an automatic assertion." [] +is_a: ECO:0001842 ! random mutagenesis of specific target DNA evidence +is_a: ECO:0007478 ! random mutagenesis evidence used in automatic assertion +property_value: IAO:0000234 "ECO" xsd:string +created_by: jbmunro +creation_date: 2019-12-09T12:00:00Z + +[Term] +id: ECO:0001844 +name: random mutagenesis of specific target DNA evidence used in manual assertion +def: "A type of random mutagenesis of specific target DNA evidence that is used in a manual assertion." [] +is_a: ECO:0001842 ! random mutagenesis of specific target DNA evidence +is_a: ECO:0005530 ! random mutagenesis evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 "ECO" xsd:string +created_by: jbmunro +creation_date: 2019-12-09T12:00:00Z + +[Term] +id: ECO:0001845 +name: cell population optical density evidence +def: "A type of cell-based assay evidence in which optical density is used to measure some aspect of a population of cells. Examples of aspects that can affect optical density measurements include, but are not limited to, cell number and cell shape." [] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 "CACAO" xsd:string +created_by: jbmunro +creation_date: 2020-01-28T12:00:00Z + +[Term] +id: ECO:0001846 +name: cell population optical density evidence used in automatic assertion +def: "A type of cell population optical density evidence that is used in an automatic assertion." [] +is_a: ECO:0001845 ! cell population optical density evidence +property_value: IAO:0000234 "CACAO" xsd:string +created_by: jbmunro +creation_date: 2020-01-28T12:00:00Z + +[Term] +id: ECO:0001847 +name: cell population optical density evidence used in manual assertion +def: "A type of cell population optical density evidence that is used in a manual assertion." [] +is_a: ECO:0001845 ! cell population optical density evidence +property_value: IAO:0000234 "CACAO" xsd:string +created_by: jbmunro +creation_date: 2020-01-28T12:00:00Z + +[Term] +id: ECO:0005004 +name: cell viability assay evidence +def: "A type of cell-based assay evidence resulting from analyzing the ability of cells to survive or live successfully." [NBK:144065] +is_a: ECO:0001565 ! cell-based assay evidence +created_by: snadendla +creation_date: 2015-02-13T12:08:01Z + +[Term] +id: ECO:0005007 +name: cell proliferation assay evidence +def: "A type of cell-based assay evidence resulting from the measurement of the number of cells, which is a reflection of the balance between cell division and cell loss through cell differentiation or cell death." [ECO:RCT] {comment="PMID:11057898", comment="GO:0008283"} +is_a: ECO:0001565 ! cell-based assay evidence +created_by: snadendla +creation_date: 2015-02-11T16:27:13Z + +[Term] +id: ECO:0005008 +name: DNA synthesis cell proliferation assay evidence +def: "A type of cell proliferation assay evidence resulting from the measure of DNA synthesis in the presece of a label." [PMC:3908118] +is_a: ECO:0005007 ! cell proliferation assay evidence +created_by: snadendla +creation_date: 2015-02-11T16:30:10Z + +[Term] +id: ECO:0005011 +name: ATP bioluminescence assay evidence +def: "A type of cell viability assay evidence resulting from the analysis of ATP concentration (by measuring light intensity) when luciferase catalyzes the oxidation of luciferin in the presence of ATP, magnesium ions and molecular oxygen." [PMID:15344559] +is_a: ECO:0005004 ! cell viability assay evidence +created_by: snadendla +creation_date: 2015-02-11T16:47:30Z + +[Term] +id: ECO:0005012 +name: cytotoxicity assay evidence +def: "A type of cell-based assay evidence resulting from the ability of an agent to induce cellular necrosis or apoptosis and the subsequent analysis of cell death mechanism." [] +is_a: ECO:0001565 ! cell-based assay evidence +created_by: snadendla +creation_date: 2015-02-12T11:39:35Z + +[Term] +id: ECO:0005014 +name: in vitro cell based assay evidence +comment: Use children of ECO:0001565 (cell-based assay evidence) in place of this term. +is_obsolete: true +created_by: snadendla +creation_date: 2015-02-12T13:26:55Z + +[Term] +id: ECO:0005019 +name: staining evidence +def: "A type of direct assay evidence resulting from the use of stains or dyes to aid in analysis of a microscopic image." [ECO:RCT] +is_a: ECO:0000002 ! direct assay evidence +created_by: snadendla +creation_date: 2015-02-19T11:48:57Z + +[Term] +id: ECO:0005021 +name: chemotaxis assay evidence +def: "A type of cell-based assay evidence resulting from the evaluation of chemotactic ability (i.e. the movement, or lack of movement, in response to a chemical stimulus) of prokaryotic or eukaryotic cells." [PMC:3667641] +is_a: ECO:0001565 ! cell-based assay evidence +created_by: snadendla +creation_date: 2015-02-19T13:10:20Z + +[Term] +id: ECO:0005027 +name: genetic transformation evidence +def: "A type of mutant phenotype evidence resulting from the conversion of one genotype into another by the introduction of exogenous DNA." [ECO:RCT] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: snadendla +creation_date: 2015-03-16T15:33:50Z + +[Term] +id: ECO:0005031 +name: structure determination evidence +def: "A type of experimental evidence resulting from the visualization and examination of chemical and/or molecular structures." [ECO:RCT] +is_a: ECO:0000006 ! experimental evidence +property_value: IAO:0000112 "The secondary structure of VapC10 (Figure S2), predicted with the 3DJIGSAW prediction tool [36] and the DALI server [37], exhibited homology with several well studied VapC toxins, such as the first PIN domain structure for the protein PAE2754 from the archae bacterium Pyrobaculum aerophilum (30)." xsd:string {xref="PMID:24260461"} +created_by: snadendla +creation_date: 2015-03-20T12:16:17Z + +[Term] +id: ECO:0005033 +name: electron microscopy evidence +def: "A type of microscopy evidence and structure determination evidence resulting from an electron beam utilized to create an image." [ECO:RCT] +is_a: ECO:0001098 ! microscopy evidence +is_a: ECO:0005031 ! structure determination evidence +property_value: IAO:0000112 "Electron micrographs showed that all the cells are rod-shaped and those of the parent strain Ye9 and the envZ mutant strain EZ10 are flagellated, but the ompR mutant cells lack flagella." xsd:string {xref="PMID:20830609"} +created_by: snadendla +creation_date: 2015-03-20T12:21:48Z + +[Term] +id: ECO:0005034 +name: apoptotic assay evidence +def: "A type of cell-based assay evidence resulting from the occurrence, visualization, and/or analysis of apoptosis." [ECO:RCT] +is_a: ECO:0001565 ! cell-based assay evidence +created_by: snadendla +creation_date: 2015-04-07T21:58:24Z + +[Term] +id: ECO:0005500 +name: in vivo polyADP-ribosylation assay evidence +def: "A type of polyADP-ribosylation assay evidence used in an in vivo experiment." [ECO:SN, PMID:2820766, SIB:PG] +is_a: ECO:0001557 ! polyADP-ribosylation assay evidence +created_by: snadendla +creation_date: 2015-06-15T15:35:05Z + +[Term] +id: ECO:0005501 +name: in vivo polyADP-ribosylation assay evidence used in manual assertion +def: "A type of in vivo polyADP-ribosylation assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005500 ! in vivo polyADP-ribosylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2015-06-15T15:37:23Z + +[Term] +id: ECO:0005502 +name: ex vivo assay evidence +def: "A type of direct assay evidence derived by studying an organ, tissues, or cells taken from an organism and studied in an external environment with the minimum alteration of natural conditions." [ECO:SN, PMID:16305312, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf] +is_a: ECO:0000002 ! direct assay evidence +created_by: snadendla +creation_date: 2015-06-26T11:46:15Z + +[Term] +id: ECO:0005503 +name: two-dimensional polyacrylamide gel electrophoresis evidence +def: "A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape." [ECO:SW, url:http\://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html] +comment: Two-dimensional polyacrylamide gel electrophoresis is commonly employed to establish a proteome for an organism and demonstrate expression changes. +synonym: "2D PAGE" EXACT [] +synonym: "2D-PAGE" EXACT [] +is_a: ECO:0000337 ! gel electrophoresis evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-01T16:23:20Z + +[Term] +id: ECO:0005504 +name: spectrometry evidence +def: "A type of experimental evidence resulting from the use of a spectrometer." [ECO:RCT] +is_a: ECO:0000006 ! experimental evidence +created_by: snadendla +creation_date: 2015-08-14T15:48:08Z + +[Term] +id: ECO:0005505 +name: regular expression motif search evidence +def: "A type of motif similarity evidence where the motif, a local alignment of sequences, is represented by a regular expression, which captures the variability of nucleotides at each position in a discrete form and can then be used to search genetic sequences by identifying matches to the regular expression." [ECO:SW, PMID:17337630] +comment: Regular expression is a computational tool used for determining whether a given genome contains a DNA region resembling a transcription factor binding motif. For example A/T=W indicates A or T may be present in a column of the alignment. (PMID: 20708667). +is_a: ECO:0000028 ! motif similarity evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-09T13:51:52Z + +[Term] +id: ECO:0005506 +name: missense mutation phenotypic evidence +def: "A type of point mutation phenotypic evidence resulting from a change in a base (or bases) causing the substitution of one amino acid for another." [SO:0001583] +synonym: "missense mutation evidence" EXACT [] +is_a: ECO:0001113 ! point mutation phenotypic evidence +created_by: snadendla +creation_date: 2015-09-18T16:52:34Z + +[Term] +id: ECO:0005507 +name: nonsense mutation phenotypic evidence +def: "A type of point mutation phenotypic evidence resulting in an incomplete and usually nonfunctional protein product because of a premature stop codon." [url:https\://ghr.nlm.nih.gov/primer/mutationsanddisorders/possiblemutations] +synonym: "nonsense mutation evidence" EXACT [] +is_a: ECO:0001113 ! point mutation phenotypic evidence +created_by: snadendla +creation_date: 2015-09-18T16:55:32Z + +[Term] +id: ECO:0005508 +name: silent mutation evidence +def: "A type of point mutation phenotypic evidence resulting from the change of a DNA nucleotide that does not cause any changes in the amino acid sequence." [url:http\://www.sciencemag.org/news/2006/12/sound-silent-mutation] +is_a: ECO:0001113 ! point mutation phenotypic evidence +created_by: snadendla +creation_date: 2015-09-18T17:01:12Z + +[Term] +id: ECO:0005509 +name: insertion mutation phenotypic evidence +def: "A type of mutant phenotype evidence resulting from a mutation in which there is an insertion of one or more contiguous nucleotides." [GO:0070705] +synonym: "insertion mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: snadendla +creation_date: 2015-09-18T17:02:03Z + +[Term] +id: ECO:0005511 +name: duplication mutation evidence +def: "A type of experimental phenotypic evidence resulting from a mutation in which inserted nucleotide(s) have a sequence identical to, or derived from, adjacent nucleotides." [SO:1000035] +is_a: ECO:0000059 ! experimental phenotypic evidence +created_by: snadendla +creation_date: 2015-09-18T17:03:25Z + +[Term] +id: ECO:0005512 +name: frameshift mutation phenotypic evidence +def: "A type of mutant phenotype evidence resulting from any mutation that causes an insertion or deletion of nucleotides in a DNA sequence that is not divisible by three and therefore changes the codon reading frame and the translated amino acids." [url:https\://ghr.nlm.nih.gov/primer/mutationsanddisorders/possiblemutations] +synonym: "frameshift mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: snadendla +creation_date: 2015-09-18T17:05:49Z + +[Term] +id: ECO:0005513 +name: repeat expansion mutation phenotypic evidence +def: "A type of mutant phenotype evidence resulting from a mutation that increases the number of times that a DNA sequence is repeated." [PMID:9397685, url:https\://ghr.nlm.nih.gov/primer/mutationsanddisorders/possiblemutations] +synonym: "repeat expansion mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: snadendla +creation_date: 2015-09-18T17:06:34Z + +[Term] +id: ECO:0005514 +name: splice site mutation phenotypic evidence +def: "A type of mutant phenotype evidence resulting from a mutation in which there is a change in nucleotides at a splice site." [ECO:RCT] +synonym: "splice site mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: snadendla +creation_date: 2015-09-18T17:07:29Z + +[Term] +id: ECO:0005515 +name: translocation mutation phenotypic evidence +def: "A type of mutant phenotype evidence resulting from a mutation in which a region of a chromosome is transferred to a nonhomologous chromosome." [ECO:RCT] +synonym: "translocation mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: snadendla +creation_date: 2015-09-18T17:08:01Z + +[Term] +id: ECO:0005516 +name: molecule detection assay evidence +def: "A type of experimental evidence resulting from the detection and analysis of molecular markers." [ECO:RCT] +is_a: ECO:0000006 ! experimental evidence +created_by: snadendla +creation_date: 2015-09-24T15:54:21Z + +[Term] +id: ECO:0005517 +name: protein detection assay evidence +def: "A type of molecule detection assay evidence resulting from the detection and quantification of a protein, or multiple proteins." [ECO:RCT] +is_a: ECO:0005516 ! molecule detection assay evidence +created_by: snadendla +creation_date: 2015-09-25T14:27:40Z + +[Term] +id: ECO:0005518 +name: RNA detection assay evidence +def: "A type of molecule detection assay evidence resulting from the detection of specific RNAs." [ECO:RCT] +is_a: ECO:0005516 ! molecule detection assay evidence +created_by: snadendla +creation_date: 2015-09-25T14:43:53Z + +[Term] +id: ECO:0005519 +name: DNA detection assay evidence +def: "A type of molecule detection assay evidence resulting from the detection and visualization of a sequence of DNA." [ECO:RCT] +is_a: ECO:0005516 ! molecule detection assay evidence +created_by: snadendla +creation_date: 2015-09-25T19:33:25Z + +[Term] +id: ECO:0005520 +name: interferometric reflectance imaging sensor evidence +def: "A type of molecule detection assay evidence based on changes in surface-reflected light due to ligand binding on the surface of a semi-transparent substrate to detect proteins, DNA, and other biological material in a multiplexed, high-throughput microarray format without labels." [ECO:SW, PMID:21587155, PMID:24271115] +comment: Differences in the optical path lengths between a surface layer and a buried silica layer can be measured very precisely allowing optical height information to be converted to accumulated mass on the surface. For the purpose of TF-binding site identification, IRIS is coupled with DNA-arrays with immobilized potential binding regions exposed to the protein of interest and eluted. Protein-bound fragments exhibit changes in reflectance that can be used to gauge binding quantitatively. +synonym: "IRIS" EXACT [] +synonym: "Spectral Reflectance Imaging Biosensor" EXACT [] +synonym: "SRIB" EXACT [] +is_a: ECO:0005516 ! molecule detection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-28T16:01:01Z + +[Term] +id: ECO:0005521 +name: S1 nuclease protection assay evidence +def: "A type of nuclease protection assay evidence where RNA is hybridized with complementary DNA probes and exposed to S1 nuclease for unbound RNA degradation after which the intact RNA is run on a gel for probe size determination and RNA identification to detect and map specific RNAs in a complex mixture of total cellular RNA." [ECO:SW, PMID:21390683] +is_a: ECO:0001106 ! nuclease protection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-28T17:16:56Z + +[Term] +id: ECO:0005522 +name: DNA dot blot assay evidence +def: "A type of DNA detection assay evidence resulting from evaluation of the relative abundance of target sequences hybridizing to DNA samples that are immobilized on a nitrocellulose or nylon membrane." [PMID:18265189] +is_a: ECO:0005519 ! DNA detection assay evidence +created_by: snadendla +creation_date: 2015-09-30T10:03:03Z + +[Term] +id: ECO:0005523 +name: DNA dot blot assay evidence used in manual assertion +def: "A type of DNA dot blot assay evidence that is used in a manual assertion." [ECO:SN, ECO:SW] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0005522 ! DNA dot blot assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2015-09-30T10:04:00Z + +[Term] +id: ECO:0005526 +name: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence +def: "A type of protein detection assay evidence where soft ionization is used for the analysis of biomolecules for mass determination in a three step process of plate preparation, radiation, and ionization with high throughput technology." [ECO:SW, PMID:21964792, PMID:25354905] +comment: MALDI-TOF MS is commonly used for protein identification in constructing a proteome where protein fingerprints obtained by microbial cells are compared with a database of reference spectra by means of various algorithms integrated in systems recently made commercially available. +synonym: "MALDI-TOF Mass Spectrometry" EXACT [] +synonym: "MALDI-TOF-MS" EXACT [] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-30T10:51:56Z + +[Term] +id: ECO:0005527 +name: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion +def: "A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in a manual assertion." [ECO:SN, ECO:SW] +synonym: "MALDI-TOF mass spectrometry evidence" BROAD [] +synonym: "MALDI-TOF-MS evidence" BROAD [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2015-09-30T10:58:51Z + +[Term] +id: ECO:0005528 +name: site-directed mutagenesis phenotypic evidence +def: "A type of mutant phenotype phenotype evidence based on target-specified mutagenesis for the characterization of various gene and protein interactions, structures, and functions where oligonucleotide primers are used to alter a nucleotide sequence." [ECO:SW, PMID:21204030, PMID:24011050, PMID:3541892] +comment: Site-directed mutagenesis is often performed in tandem with EMSA. It is often implemented only in conserved motif positions or serially through all positions of a site. +synonym: "site directed mutagenesis" EXACT [] +synonym: "site-directed mutagenesis evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000112 "If the AGAA sequence is mutated CTCC (Pxyn2aM, see Table 1), no DNA-protein complex is formed under either repressing or inducing conditions (Figure 1). This result indicates that this part of the xyn2 promoter is essential for binding a transcription factor under repressing conditions." xsd:string {xref="PMID:21087492"} +property_value: IAO:0000112 "To identify key residues required for specific GlnR binding we mutated the highly conserved AC-n9-AC and AT-n9-AC DNA binding motifs. Figure 4 shows that the highly conserved adenosine residues in the motif are critical as GlnR binding is abolished when these residues are mutated." xsd:string {xref="PMID:23642041"} +property_value: IAO:0000112 "We also tested a hyaS promoter in which one (highest score) of the three putative AdpA-binding sites was mutated (at position -134 to -129, see Additional file 3: Figure S1a): the affinity of AdpA for this promoter region was reduced and one protein-DNA complex disappeared (Additional file 3: Figure S1b). These results suggest that one dimer of AdpA binds the adjacent sites -129 and -123 of S. lividans hyaS promoter and another dimer binds the -100 site resulting in the formation of the two DNA-AdpA complexes depicted in Figure 2." xsd:string {xref="PMID:24694298"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-30T11:34:41Z + +[Term] +id: ECO:0005529 +name: random mutagenesis phenotypic evidence +def: "A type of mutant phenotype evidence where a mutagenic product or process is used to alter the nucleotide sequence and thus induce genetic mutation. Examples include, but are not limited to mutagenic compounds, irradiation, PCR w/ degenerate primers, etc." [ECO:SW, PMID:15153637, PMID:18265275] +comment: Random mutagenesis is not often used in TF-binding site determination; however, it is sometimes used to investigate the effect of mutations in TF binding domains in conjunction with expression assays. Random mutations can also be introduced via chemicals, error-prone PCR (EP-PCR), degenerate oligonucleotides-Pfu(DOP), UV irradiation, mutator strains, nucleotide analogs, or DNA recombination. +synonym: "random mutagenesis evidence" NARROW [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-30T11:55:03Z + +[Term] +id: ECO:0005530 +name: random mutagenesis evidence used in manual assertion +def: "A type of random mutagenesis phenotypic evidence that is used in a manual assertion." [] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005529 ! random mutagenesis phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +created_by: snadendla +creation_date: 2015-09-30T11:57:00Z + +[Term] +id: ECO:0005531 +name: motif discovery evidence +def: "A type of multiple sequence alignment evidence based on a set of algorithms that infer over-represented motifs from a set of biological sequences returning one or more local multiple sequence alignment defining the inferred motifs." [ECO:SW, PMID:10812473, PMID:16845028, PMID:8211139] +comment: Relationship type between sequences (e.g. orthologs from multiple genomes or co-expressed genes in the same genome) is not necessarily assumed. The local multiple sequence alignment is typically ungapped. A common application of motif discovery is to infer the binding motif of a given transcription factor (TF). This can be based on the results of a whole-genome expression analysis, assuming that co-expressed genes are co-regulated by the same TF, from peaks in ChIP-seq experiments with the TF, or from the comparative genomics analysis of the promoter regions of orthologs. {xref="PMID:19892760", xref="PMID:23032607"} +is_a: ECO:0005555 ! multiple sequence alignment evidence +property_value: IAO:0000112 "A sequence alignment of the phlA gene promoter region from strain 2P24 with other well-studied 2,4-DAPG producing Pseudomonas strains revealed a very well conserved palindromic sequence GAAACN5GTTTC (Fig. S1)." xsd:string {xref="PMID:23209661"} +property_value: IAO:0000112 "In order to identify a potential regulatory motif for KstR2, we used the promoter regions of the kstR2 orthologues as a training set for the motif identification program MEME (Bailey & Elkan, 1994). This identified a potential regulatory sequence that contains a 14 bp inverted palindromic motif AnCAAGnnCTTGnT (Fig. 2)." xsd:string {xref="PMID:20167624"} +property_value: IAO:0000112 "We mapped sites upstream of crp itself, SCO4561 and SCO2977, and identified a consensus binding sequence [GTG(N)6GNCAC]; derivatives of this motif could be found in all of the secondary metabolism-associated target sequences, although notably, one-half of the palindrome seemed to be better conserved than the other [GTG(N)6GNGAN] (Fig. 2C; Table 1)." xsd:string {xref="PMID:23232715"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-30T15:04:45Z + +[Term] +id: ECO:0005532 +name: consensus search evidence +def: "A type of motif similarity evidence where the motif is represented by a consensus sequence which incorporates the most frequent nucleotide at each position acting as a generic representative for the sequences in the alignment, and is compared to the genetic sequence for a perfect or imperfect match." [ECO:SW, PMID:15130839] +comment: This technique is used to identify transcription factor binding sites by searching a bacterial genome for DNA regions resembling a given TF-binding motif. PMID: 17085555. +is_a: ECO:0000028 ! motif similarity evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-09T14:12:54Z + +[Term] +id: ECO:0005533 +name: phylogenetic footprinting evidence +def: "A type of motif discovery evidence based on a set of algorithms that infer motifs in non-coding DNA sequences from the conservation of functional regulatory sites through evolution at a higher rate than their local surroundings for the identification of an evolutionary 'footprint'." [ECO:SW, PMID:16477324, PMID:3199442] +comment: The name derives to experimental footprinting techniques where transcription factor (TF)-binding sites are identified by sequencing the region of DNA protected from biochemical agents by the bound TF. This technique is used to identify or verify transcription factor-binding motifs, most commonly in the Eukaryota (PMID: 11997340). +is_a: ECO:0005531 ! motif discovery evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-30T16:23:02Z + +[Term] +id: ECO:0005534 +name: comparative genomics motif search evidence +def: "A type of motif similarity evidence based on comparative genomics which uses a search method to scan a set of genomes for instances of a specific motif, and then applies a comparative criterion to analyze the collective strength of these predictions, based on the assumption that functional instances of the motif will be preserved by natural selection and their prediction will therefore be consistent across genomes." [ECO:SW, PMID:10854408] +comment: This technique is used to predict transcription factor binding sites in gene promoter regions by identifying conserved DNA regions across multiple genomes. PMID: 22305460. Orthology is a typical comparative criterion used. +is_a: ECO:0000028 ! motif similarity evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-09T14:30:38Z + +[Term] +id: ECO:0005535 +name: machine learning prediction of motif instance evidence +def: "A type of match to sequence model evidence which captures the application of a broad suite of supervised and unsupervised machine learning algorithms trained on known motif instances and applied to the prediction of novel putative instances of a motif in a set of biological sequences." [ECO:SW, PMID:17117497, PMID:2014171] +comment: Machine learning algorithms include, but are not limited to artificial neural networks, random-forest, k-means clustering, hidden Markov models. This suite of methods can be used to identify or verify transcription factor-binding sites in genomic sequences (PMID: 15703297). +is_a: ECO:0000202 ! match to sequence model evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-01T11:58:36Z + +[Term] +id: ECO:0005536 +name: position-specific scoring matrix motif search evidence +def: "A type of motif similarity evidence where the motif is represented by a position-specific frequency matrix (PSFM) that specifies the frequency of each base at each position of the motif, from which a position-specific scoring matrix (PSSM) can be derived under the framework of a log-likelihood ratio providing a scoring system based on the likelihood of a sequence given the motif, versus a genomic-frequency based null hypothesis." [ECO:SW, PMID:10812473] +comment: This technique is used to predict transcription factor binding sites in gene promoter regions by using a position weight matrix approach. (PMID: 15604457). +synonym: "PSSM" NARROW [] +synonym: "PSSM motif search" EXACT [] +is_a: ECO:0000028 ! motif similarity evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-09T15:04:35Z + +[Term] +id: ECO:0005537 +name: xylE reporter gene assay evidence +def: "A type of reporter gene assay evidence based on the fusion of the xylE gene to a specific promoter for the expression of catechol 2,3-dioxygenase that converts the colorless catechol substrate to yellow 2-hydroxymuconic semialdehyde for identification of the expression of a particular gene." [ECO:SW, PMID:2592344] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-28T15:31:28Z + +[Term] +id: ECO:0005538 +name: computationally derived logical inference +is_obsolete: true +created_by: snadendla +creation_date: 2015-11-17T15:15:50Z + +[Term] +id: ECO:0005539 +name: computationally derived logical inference used in automatic assertion +is_obsolete: true +created_by: snadendla +creation_date: 2015-11-17T15:17:47Z + +[Term] +id: ECO:0005540 +name: computationally derived logical inference from automatic assertion used in automatic assertion +is_obsolete: true +created_by: snadendla +creation_date: 2015-11-17T15:24:42Z + +[Term] +id: ECO:0005541 +name: computationally derived logical inference from manual assertion used in automatic assertion +is_obsolete: true +created_by: snadendla +creation_date: 2015-11-17T15:32:03Z + +[Term] +id: ECO:0005542 +name: biological system reconstruction evidence by experimental evidence from single species used in manual assertion +def: "A type of biological system reconstruction evidence by experimental evidence from single species that is used in a manual assertion." [ECO:SN, PMID:19450514] +comment: Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof. +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0005553 ! biological system reconstruction evidence by experimental evidence from single species +created_by: snadendla +creation_date: 2015-12-16T15:32:41Z + +[Term] +id: ECO:0005543 +name: biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion +def: "A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in a manual assertion." [ECO:SN, PMID:22232657] +comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments. +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0005552 ! biological system reconstruction evidence by experimental evidence from mixed species +created_by: snadendla +creation_date: 2015-12-16T15:45:01Z + +[Term] +id: ECO:0005544 +name: biological system reconstruction evidence based on orthology evidence used in manual assertion +def: "A type of biological system reconstruction evidence based on orthology evidence that is used in a manual assertion." [ECO:SN] +comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. +is_a: ECO:0005545 ! biological system reconstruction evidence based on orthology evidence +is_a: ECO:0005610 ! biological system reconstruction evidence based on homology evidence used in manual assertion +created_by: snadendla +creation_date: 2015-12-18T16:20:49Z + +[Term] +id: ECO:0005545 +name: biological system reconstruction evidence based on orthology evidence +def: "A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by orthology from an existing experimentally supported model to a process, pathway or complex in another species." [ECO:SN, PMID:15660128] +comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. +is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence +created_by: snadendla +creation_date: 2015-12-16T15:57:37Z + +[Term] +id: ECO:0005546 +name: biological system reconstruction evidence based on paralogy evidence used in manual assertion +def: "A type of biological system reconstruction evidence based on paralogy evidence that is used in a manual assertion." [ECO:SN, PMID:15660128] +comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. +is_a: ECO:0005550 ! biological system reconstruction evidence based on paralogy evidence +is_a: ECO:0005610 ! biological system reconstruction evidence based on homology evidence used in manual assertion +created_by: snadendla +creation_date: 2015-12-16T16:05:15Z + +[Term] +id: ECO:0005547 +name: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion +def: "A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in a manual assertion." [ECO:SN, PMID:17876790] +comment: Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components. +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0005548 ! biological system reconstruction evidence based on inference from background scientific knowledge +created_by: snadendla +creation_date: 2015-12-16T16:07:42Z + +[Term] +id: ECO:0005548 +name: biological system reconstruction evidence based on inference from background scientific knowledge +def: "A type of biological system reconstruction based solely on background scientific knowledge of that biological system." [ECO:SN, PMID:17876790] +comment: The available knowledge is usually a combination of partial or weak experimental evidence where either some components are missing or no physical interaction evidence can be found but the system is inferred by similarity to related systems in taxonomically-disparate organisms with experimental evidence. Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components. +is_a: ECO:0000088 ! biological system reconstruction evidence +created_by: snadendla +creation_date: 2015-12-18T15:32:15Z + +[Term] +id: ECO:0005549 +name: biological system reconstruction evidence based on homology evidence +def: "A type of biological system reconstruction where the evidence is inferred by homology based on conservation of sequence, function, and composition from an existing experimentally supported model to a process, pathway, or complex." [ECO:SN, PMID:15660128] +comment: Inference may be based on paralogy and/or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments in the same or another species. +is_a: ECO:0000088 ! biological system reconstruction evidence +created_by: snadendla +creation_date: 2015-12-18T15:49:46Z + +[Term] +id: ECO:0005550 +name: biological system reconstruction evidence based on paralogy evidence +def: "A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by paralogy from an existing experimentally supported model to a process, pathway, or complex in the same species." [ECO:SN, PMID:15660128] +comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. +is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence +created_by: snadendla +creation_date: 2015-12-18T16:24:08Z + +[Term] +id: ECO:0005551 +name: biological system reconstruction evidence by experimental evidence +def: "A type of biological system reconstruction evidence that uses experimental evidence as support." [ECO:SN, PMID:19450514, PMID:22232657] +is_a: ECO:0000088 ! biological system reconstruction evidence +created_by: snadendla +creation_date: 2015-12-18T16:34:13Z + +[Term] +id: ECO:0005552 +name: biological system reconstruction evidence by experimental evidence from mixed species +def: "A type of biological system reconstruction evidence where the experimental evidence is derived by using a mix of species used in the same experiment." [ECO:SN, PMID:22232657] +comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments. +is_a: ECO:0005551 ! biological system reconstruction evidence by experimental evidence +created_by: snadendla +creation_date: 2015-12-18T16:35:13Z + +[Term] +id: ECO:0005553 +name: biological system reconstruction evidence by experimental evidence from single species +def: "A type of biological system reconstruction where the experimental evidence is derived by using multiple experiments in a single species." [ECO:SN] +comment: Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof. +is_a: ECO:0005551 ! biological system reconstruction evidence by experimental evidence +created_by: snadendla +creation_date: 2015-12-18T16:36:44Z + +[Term] +id: ECO:0005554 +name: pairwise sequence alignment evidence +def: "A type of sequence alignment evidence where two sequences are aligned to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences (protein or nucleic acid)." [ECO:SN, url:http\://www.ebi.ac.uk/Tools/psa/] +is_a: ECO:0000200 ! sequence alignment evidence +created_by: snadendla +creation_date: 2016-02-08T14:30:46Z + +[Term] +id: ECO:0005555 +name: multiple sequence alignment evidence +def: "A type of sequence alignment evidence where three or more biological sequences (protein or nucleic acid) of similar length are aligned to infer homology and the evolutionary relationships between the sequences studied." [ECO:SN, url:http\://www.ebi.ac.uk/Tools/msa/] +is_a: ECO:0000200 ! sequence alignment evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2016-02-08T14:31:24Z + +[Term] +id: ECO:0005556 +name: multiple sequence alignment evidence used in manual assertion +def: "A type of multiple sequence alignment evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000247 ! sequence alignment evidence used in manual assertion +is_a: ECO:0005555 ! multiple sequence alignment evidence +property_value: ECO:9000002 "ISA" xsd:string +created_by: snadendla +creation_date: 2016-02-08T14:59:18Z + +[Term] +id: ECO:0005557 +name: multiple sequence alignment evidence used in automatic assertion +def: "A type of multiple sequence alignment evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0000248 ! sequence alignment evidence used in automatic assertion +is_a: ECO:0005555 ! multiple sequence alignment evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-02-08T15:00:50Z + +[Term] +id: ECO:0005558 +name: motif discovery evidence used in manual assertion +def: "A type of motif discovery evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005531 ! motif discovery evidence +is_a: ECO:0005556 ! multiple sequence alignment evidence used in manual assertion +property_value: ECO:9000002 "ISA" xsd:string +created_by: snadendla +creation_date: 2016-02-08T15:07:47Z + +[Term] +id: ECO:0005559 +name: motif discovery evidence used in automatic assertion +def: "A type of motif discovery evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0005531 ! motif discovery evidence +is_a: ECO:0005557 ! multiple sequence alignment evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-02-08T15:08:19Z + +[Term] +id: ECO:0005560 +name: pairwise sequence alignment evidence used in manual assertion +def: "A type of pairwise sequence alignment evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000247 ! sequence alignment evidence used in manual assertion +is_a: ECO:0005554 ! pairwise sequence alignment evidence +property_value: ECO:9000002 "ISA" xsd:string +created_by: snadendla +creation_date: 2016-02-08T15:17:05Z + +[Term] +id: ECO:0005561 +name: pairwise sequence alignment evidence used in automatic assertion +def: "A type of pairwise sequence alignment evidence that is used in an automatic evidence." [ECO:SN] +is_a: ECO:0000248 ! sequence alignment evidence used in automatic assertion +is_a: ECO:0005554 ! pairwise sequence alignment evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-02-08T15:17:52Z + +[Term] +id: ECO:0005562 +name: in-gel protein kinase assay evidence +def: "A type of protein kinase assay evidence resulting from the evaluation of the phosphorylation activity of kinase on a gel-incorporated kinase substrate." [PMID:12372853] +is_a: ECO:0001558 ! protein kinase assay evidence +created_by: snadendla +creation_date: 2016-02-09T12:05:29Z + +[Term] +id: ECO:0005563 +name: macroscopic current trace evidence +def: "A type of voltage clamp recording evidence that involves recording trace of the current (pA) going through the analyzed ion channels determined from voltage clamp current recording." [ECO:SN, PMID:17938230, url:http\://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch03.pdf] +is_a: ECO:0005576 ! voltage clamp recording evidence +created_by: snadendla +creation_date: 2016-02-09T12:40:03Z + +[Term] +id: ECO:0005564 +name: current density evidence +def: "A type of electrophysiology assay evidence that involves measuring the amount of current (pA) going through the ion channels per unit of membrane." [ECO:SN, url:http\://circres.ahajournals.org/content/102/11/1298.full, url:http\://www.bem.fi/book/04/04.htm] +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: snadendla +creation_date: 2016-02-09T12:43:07Z + +[Term] +id: ECO:0005565 +name: single channel conductance evidence +is_obsolete: true +created_by: snadendla +creation_date: 2016-02-09T12:45:42Z + +[Term] +id: ECO:0005566 +name: sustained current evidence +def: "A type of electrophysiology assay evidence that involves the measurement of the current that persists after the fast and slow inactivation of the analyzed channels." [ECO:SN, url:http\://www.nature.com/ncomms/journal/v3/n2/full/ncomms1717.html] +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: snadendla +creation_date: 2016-02-09T12:46:54Z + +[Term] +id: ECO:0005567 +name: steady state activation curve evidence +is_obsolete: true +created_by: snadendla +creation_date: 2016-02-09T12:48:17Z + +[Term] +id: ECO:0005568 +name: steady state inactivation curve evidence +is_obsolete: true +created_by: snadendla +creation_date: 2016-02-09T12:50:45Z + +[Term] +id: ECO:0005569 +name: window current trace evidence +is_obsolete: true +created_by: snadendla +creation_date: 2016-02-09T12:57:16Z + +[Term] +id: ECO:0005570 +name: use dependence of inactivation evidence +def: "A type of electrophysiology assay evidence in which the amount of current remaining after a series of depolarization at different frequencies described as the normalized current related to the time course." [ECO:SN, url:http\://circres.ahajournals.org/content/89/8/700.full] +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: snadendla +creation_date: 2016-02-09T12:58:18Z + +[Term] +id: ECO:0005571 +name: current clamp recording evidence +def: "A type of electrophysiology assay evidence that involves recording the change in membrane potential caused by applying specific current to the cell." [ECO:SN, url:http\://www.nature.com/nprot/journal/v4/n8/full/nprot.2009.91.html] +synonym: "current clamp voltage recording evidence" EXACT [] +xref: birnlex:2278 "current clamp voltage recording protocol" +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: snadendla +creation_date: 2016-02-12T13:58:32Z + +[Term] +id: ECO:0005572 +name: whole-cell voltage clamp recording evidence +def: "A type of voltage clamp recording evidence that involves direct measurement of ionic current across a membrane while controlling the membrane potential by maintaining the voltage." [ECO:SN, url:http\://circres.ahajournals.org/content/72/1/91.full.pdf] +xref: birnlex:2281 "whole-cell voltage clamp recording protocol" +is_a: ECO:0005576 ! voltage clamp recording evidence +created_by: snadendla +creation_date: 2016-02-12T14:06:40Z + +[Term] +id: ECO:0005573 +name: cell-attached single-channel recording evidence +def: "A type of patch-clamp recording evidence that involves isolation of a patch of membrane electrically from the external solution and to record current flowing into the patch and this is achieved by pressing a fire-polished glass pipette, which has been filled with a suitable electrolyte solution, against the surface of a cell and applying light suction." [ECO:SN, url:http\://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch04.pdf] +synonym: "cell-attached patch-clamp recording evidence" EXACT [] +xref: birnlex:2283 "cell-attached single-channel recording protocol" +is_a: ECO:0006012 ! patch-clamp recording evidence +created_by: snadendla +creation_date: 2016-02-12T14:07:39Z + +[Term] +id: ECO:0005574 +name: cell-detached inside-out single-channel recording evidence +def: "A type of patch-clamp recording evidence that involves study of individual ion channels by gently pulling the patch of membrane away from the cell, and the patch remains attached to the pipette with its cytoplasmic surface exposed to the bathing solution." [ECO:SN, url:http\://www.acnp.org/g4/GN401000005/CH005.html] +synonym: "cell-detached inside-out patch-clamp recording evidence" EXACT [] +xref: birnlex:2286 "cell-detached inside-out single-channel recording protocol" +is_a: ECO:0006012 ! patch-clamp recording evidence +created_by: snadendla +creation_date: 2016-02-12T14:16:53Z + +[Term] +id: ECO:0005575 +name: reconstituted bilayer single-channel patch recording evidence +def: "A type of patch-clamp reocrding evidence that involves ion channel analysis by incorporating the ion channels into liposomes suitable for patch-clamping which allows isolation and study of a specific population of channels into that bilayer membrane." [ECO:SN, url:http\://nanion.de/images/stories/papers/Bilayers_lab-on-a-chip08.pdf] +xref: birnlex:2287 "reconstituted bilayer single-channel patch recording protocol" +is_a: ECO:0006012 ! patch-clamp recording evidence +created_by: snadendla +creation_date: 2016-02-12T14:17:46Z + +[Term] +id: ECO:0005576 +name: voltage clamp recording evidence +def: "A type of electrophysiology assay evidence that involves recording membrane potential by controlling the membrane voltage and measuring the transmembrane current required to maintain that voltage." [ECO:SN, url:http\://neurobio.drexelmed.edu/GaoWeb/Resources/Recording%20_Techniques.pdf, url:http\://www.acnp.org/g4/GN401000005/CH005.html] +synonym: "voltage clamp current recording evidence" EXACT [] +xref: birnlex:2279 "voltage clamp current recording protocol" +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: snadendla +creation_date: 2016-02-12T14:18:36Z + +[Term] +id: ECO:0005577 +name: electroencephalography recording evidence +def: "A type of electrophysiology assay evidence that involves recording the electrical activity of the brain by means of electrodes placed on the surface of the head." [ECO:SN, url:http\://www.jove.com/science-education/5420/electro-encephalography-eeg] +xref: birnlex:2293 "electroencephalography recording protocol" +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: snadendla +creation_date: 2016-02-12T14:19:21Z + +[Term] +id: ECO:0005578 +name: super-resolution microscopy evidence +def: "A type of microscopy evidence where the images are taken with a higher resolution than the diffraction limit that help in studying nanoscopic sub-cellular structures." [ECO:SN, url:http\://journal.frontiersin.org/article/10.3389/fncel.2015.00007/full] +is_a: ECO:0001098 ! microscopy evidence +created_by: snadendla +creation_date: 2016-02-12T15:20:47Z + +[Term] +id: ECO:0005579 +name: immunogold labelling evidence used in manual assertion +def: "A type of immunogold labelling evidence that is used in a manual assertion." [ECO:SN] +synonym: "immunogold labelling" BROAD [] +is_a: ECO:0000090 ! immunogold labelling evidence +is_a: ECO:0006031 ! immunolocalization evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-02-12T15:38:03Z + +[Term] +id: ECO:0005580 +name: flow cytometry evidence used in manual assertion +def: "A type of flow cytometry evidence that is used in a manual assertion." [ECO:SN] +synonym: "FCM" BROAD [] +is_a: ECO:0000268 ! flow cytometry evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-02-12T16:04:23Z + +[Term] +id: ECO:0005581 +name: enzyme-linked immunoabsorbent assay evidence used in manual assertion +def: "A type of enzyme-linked immunoabsorbent assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "ELISA evidence|enzyme-linked immunosorbent assay evidence" BROAD [] +is_a: ECO:0000267 ! enzyme-linked immunoabsorbent assay evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-02-12T16:23:38Z + +[Term] +id: ECO:0005582 +name: cell-detached outside-out single-channel recording evidence +def: "A type of patch-clamp recording evidence where a part of cellular membrane is detached from the cell and whose external face is exposed to the extracellular medium which allows isolating and studying a single or at least a small number of channels into that fragment (patch)." [ECO:SN, url:http\://www.nature.com/nprot/journal/v2/n11/full/nprot.2007.403.html] +synonym: "cell-detached outside-out patch-clamp evidence" EXACT [] +is_a: ECO:0006012 ! patch-clamp recording evidence +created_by: snadendla +creation_date: 2016-03-02T10:47:25Z + +[Term] +id: ECO:0005583 +name: cut-open oocyte voltage clamp recording evidence +def: "A type of voltage clamp recording evidence that involves inserting the oocyte in a chamber that separates the surface into 3 regions where the top portion of the oocyte membrane is the region that is clamped from which currents are actually recorded while the bottom portion is the region of the oocyte that is cut-open, making it possible to inject current intracellularly through a low resistance pathway." [ECO:SN, PMID:9711615] +is_a: ECO:0005576 ! voltage clamp recording evidence +created_by: snadendla +creation_date: 2016-03-02T11:29:32Z + +[Term] +id: ECO:0005584 +name: macropatch voltage clamp recording evidence +def: "A type of voltage clamp recording evidence where recording is done on a fragment of the cell membrane (attached to or detached from the cell) using a large tip diameter pipette that allows isolating and studying a small number of channels into that fragment (patch)." [ECO:SN, url:http\://jxb.oxfordjournals.org/content/50/Special_Issue/1037.full.pdf] +is_a: ECO:0005576 ! voltage clamp recording evidence +created_by: snadendla +creation_date: 2016-03-02T11:33:48Z + +[Term] +id: ECO:0005585 +name: high throughput mass spectrometry evidence +def: "A type of mass spectrometry evidence where mass-to-charge ratio (m/z) of gas-phase ions is measured which is used to identify and quantify molecules in complex solutions within the framework of high throughput studies." [ECO:SN, PMID:20805795] +comment: There are significant differences in data analysis and data evaluation if to compare a HTP mass-spec and a regular mass-spec. The regular mass spec method is quite a reliable technique for identifying a protein molecular weight and analysing its post-modifications, other mass-spec applications might require the additional quality controls and more stringent data evaluation. More complex sample mixture is processed, more quality questions should be addressed, such as a sample contamination and sensitivity of the mass spectrometer while using HTP mass-spec.\nFor HTP mass-spec, the analysis of the small data sets allows to manually assign the spectra to proteins or peptides, but for large data sets only the statistical tools can be used instead. +is_a: ECO:0001096 ! mass spectrometry evidence +created_by: snadendla +creation_date: 2016-03-02T12:33:07Z + +[Term] +id: ECO:0005586 +name: high throughput mass spectrometry evidence used in manual assertion +def: "A type of high throughput mass spectrometry evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001230 ! mass spectrometry evidence used in manual assertion +is_a: ECO:0005585 ! high throughput mass spectrometry evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-03-02T12:50:02Z + +[Term] +id: ECO:0005587 +name: confocal microscopy evidence +def: "A type of microscopy evidence where sharp images are taken by excluding most of the light from the specimen that is not from the microscope's focal plane, thus allowing better contrast and less haze, which makes it possible to build three-dimensional reconstructions of a volume of the specimen by assembling a series of thin slices taken along the vertical axis." [ECO:SN, url:http\://www.physics.emory.edu/faculty/weeks//lab/papers/ebbe05.pdf] +is_a: ECO:0001098 ! microscopy evidence +created_by: snadendla +creation_date: 2016-03-17T12:29:45Z + +[Term] +id: ECO:0005588 +name: wide-field microscopy evidence +def: "A type of microscopy evidence where imaging of a thin cell or a tissue specimen is performed by full aperture emission of light gathered by the microscope's objective, which maximizes the recorded signal and simultaneously minimizes the required exposure times." [ECO:SN, url:http\://jcb.rupress.org/content/172/1/9.full, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048960/, url:http\://zeiss-campus.magnet.fsu.edu/articles/livecellimaging/techniques.html] +is_a: ECO:0001098 ! microscopy evidence +created_by: snadendla +creation_date: 2016-03-17T12:30:27Z + +[Term] +id: ECO:0005589 +name: confocal microscopy evidence used in manual assertion +def: "A type confocal microscopy evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001232 ! microscopy evidence used in manual assertion +is_a: ECO:0005587 ! confocal microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-03-17T12:32:24Z + +[Term] +id: ECO:0005590 +name: wide-field microscopy evidence used in manual assertion +def: "A type of wide-field microscopy evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001232 ! microscopy evidence used in manual assertion +is_a: ECO:0005588 ! wide-field microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-03-17T12:34:42Z + +[Term] +id: ECO:0005591 +name: immunogold labelling electron microscopy assay evidence +def: "A type of immunogold labelling evidence where electron microscopy technique is used to detect the location of the antibodies labeled with colloidal gold particles." [ECO:SN, PMID:4110101] +is_a: ECO:0000090 ! immunogold labelling evidence +is_a: ECO:0005033 ! electron microscopy evidence +created_by: snadendla +creation_date: 2016-03-17T15:43:00Z + +[Term] +id: ECO:0005592 +name: immunogold labelling electron microscopy assay evidence used in manual assertion +def: "A type of immunogold labelling electron microscopy assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005579 ! immunogold labelling evidence used in manual assertion +is_a: ECO:0005591 ! immunogold labelling electron microscopy assay evidence +is_a: ECO:0006003 ! electron microscopy evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-03-17T16:39:37Z + +[Term] +id: ECO:0005593 +name: immunodetection assay evidence +def: "A type of direct assay evidence that involves the use of antibodies to detect biomolecules." [ECO:SN] +is_a: ECO:0000002 ! direct assay evidence +created_by: snadendla +creation_date: 2016-03-17T17:07:33Z + +[Term] +id: ECO:0005594 +name: immunoperoxidase immunolocalization evidence +def: "A type of immunolocalization evidence involving enzymatic detection of a peroxidase tagged antibody." [ECO:SN] +is_a: ECO:0000087 ! immunolocalization evidence +created_by: snadendla +creation_date: 2016-03-17T17:11:34Z + +[Term] +id: ECO:0005595 +name: immunoperoxidase immunolocalization evidence used in manual assertion +def: "A type of immunoperoxidase immunolocalization evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005594 ! immunoperoxidase immunolocalization evidence +is_a: ECO:0006031 ! immunolocalization evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-03-17T17:14:05Z + +[Term] +id: ECO:0005596 +name: immunoperoxidase immunolocalization electron microscopy evidence +def: "A type of immunoperoxidase immunolocalization evidence where electron microscopy technique is used for enzymatic detection of a peroxidase tagged antibody." [ECO:SN] +synonym: "Immunoperoxidase labelling electron microscopy" EXACT [] +is_a: ECO:0005033 ! electron microscopy evidence +is_a: ECO:0005594 ! immunoperoxidase immunolocalization evidence +created_by: snadendla +creation_date: 2016-03-17T17:16:38Z + +[Term] +id: ECO:0005597 +name: immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion +def: "A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "immunoperoxidase labelling electron microscopy evidence" BROAD [] +is_a: ECO:0005595 ! immunoperoxidase immunolocalization evidence used in manual assertion +is_a: ECO:0005596 ! immunoperoxidase immunolocalization electron microscopy evidence +is_a: ECO:0006003 ! electron microscopy evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-03-17T17:23:56Z + +[Term] +id: ECO:0005598 +name: wide-field fluorescence microscopy evidence +def: "A type of wide-field microscopy evidence where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter." [ECO:SN, url:http\://www.bristol.ac.uk/synaptic/research/techniques/widefield.html] +comment: The optical filter is used to select the wavelength of excitation light. The detection camera is typically a CCD camera. Both topographical and dynamic information of a sample are obtained. +is_a: ECO:0005588 ! wide-field microscopy evidence +created_by: snadendla +creation_date: 2016-03-18T11:53:56Z + +[Term] +id: ECO:0005599 +name: immunofluorescence wide-field microscopy evidence +def: "A type of wide-field fluorescence microscopy evidence where fluorescently tagged antibodies are imaged that are used to bind to their antigens." [ECO:SN, url:http\://journals.plos.org/plosone/article?id=10.1371/journal.pone.0057135] +synonym: "wide-field epifluorescence microscopy" EXACT [] +is_a: ECO:0000007 ! immunofluorescence evidence +is_a: ECO:0005598 ! wide-field fluorescence microscopy evidence +created_by: snadendla +creation_date: 2016-03-18T11:55:40Z + +[Term] +id: ECO:0005600 +name: immunofluorescence confocal microscopy evidence +def: "A type of confocal microscopy evidence where light passes through immunofluorescent-stained tissue sections or cells and then the emitted light passes through the dichroic mirror and is focused onto the pinhole for subsequent detection." [ECO:SN, url:http\://www.physics.emory.edu/faculty/weeks/confocal/, url:https\://www.hycultbiotech.com/media/wysiwyg/protocol_Immunofluorescence.pdf] +comment: This allows visualization of sub cellular distribution of biomolecules of interest. +is_a: ECO:0000007 ! immunofluorescence evidence +is_a: ECO:0005587 ! confocal microscopy evidence +property_value: IAO:0000112 "The endogenous protein expression and localization for WalR and WalK was also checked using confocal immunofluorescence microscopy. WalR could be localized to the cytoplasm of B. anthracis but WalK could not be detected once again (data not shown)." xsd:string {xref="PMID:24490131"} +created_by: snadendla +creation_date: 2016-03-18T12:48:42Z + +[Term] +id: ECO:0005601 +name: immunofluorescence confocal microscopy evidence used in manual assertion +def: "A type of immunofluorescence confocal microscopy evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005589 ! confocal microscopy evidence used in manual assertion +is_a: ECO:0005600 ! immunofluorescence confocal microscopy evidence +is_a: ECO:0005804 ! immunofluorescence evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-03-18T12:52:04Z + +[Term] +id: ECO:0005603 +name: protein mass spectrometry evidence +def: "A type of mass spectrometry evidence where one or more protein analyses are performed." [ECO:SN] +is_a: ECO:0001096 ! mass spectrometry evidence +created_by: snadendla +creation_date: 2016-03-18T13:11:37Z + +[Term] +id: ECO:0005604 +name: cross-streak test evidence +def: "A type of cell proliferation assay evidence where the investigated organism or a potential antagonist is inoculated so as to grow along the diameter line of the agar medium in a Petri dish, then, potentially susceptible microbes (usually bacteria) are streaked perpendicularly to the growth line and inhibition of growth of a test microbe in the vicinity of that line suggests that the antagonist secretes one or more diffusible antibiotics." [ECO:SN, url:www.jstor.org/stable/3792785] +is_a: ECO:0005007 ! cell proliferation assay evidence +created_by: snadendla +creation_date: 2016-03-29T15:40:54Z + +[Term] +id: ECO:0005605 +name: disk diffusion test evidence +def: "A type of zone of inhibition evidence where sensitivity/resistance to an antimicrobial agent is tested by impregnating small filter disks with a known concentration of antimicrobial agent which is then placed on a Mueller-Hinton agar plate inoculated with the test organism." [ECO:SN, url:http\://www.cdc.gov/nczved/resources/cholera/ch9.pdf] +is_a: ECO:0007083 ! zone of inhibition evidence +property_value: IAO:0000112 "Disk diffusion assays were used to determine if ohr and ohrR mutations affected resistance to ROS. The ohr mutant was less resistant than its parental strain when challenged with organic peroxides as shown by the zones of growth inhibition: 4.1 +- 0.2 cm for CuOOH and 3.1 +- 0.1 cm for tBOOH versus to 2.3 +- 0.2 and 2.5 +- 0.3 cm for wild type strain." xsd:string {xref="PMID:21569462"} +created_by: snadendla +creation_date: 2016-03-29T16:02:22Z + +[Term] +id: ECO:0005606 +name: cell transfection experiment evidence +def: "A type of experimental evidence where a foreign genetic material is introduced into the cell for study of gene function and regulation and protein function." [ECO:SN, url:http\://ch.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/transfection/, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911531/] +is_a: ECO:0000006 ! experimental evidence +created_by: snadendla +creation_date: 2016-03-29T16:59:17Z + +[Term] +id: ECO:0005607 +name: tethered cell assay evidence +def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by observing the rotational behavior of the cells tethered to a coverslip by their flagella." [ECO:SN, PMID:1103143] +is_a: ECO:0005021 ! chemotaxis assay evidence +created_by: snadendla +creation_date: 2016-03-31T15:51:10Z + +[Term] +id: ECO:0005608 +name: tumble frequency assay evidence +def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by measuring chemotaxis in response to sudden concentration changes from one uniform spatial environment to another." [ECO:SN, PMID:4576832] +synonym: "Temporal assay" EXACT [] +is_a: ECO:0005021 ! chemotaxis assay evidence +created_by: snadendla +creation_date: 2016-03-31T16:42:35Z + +[Term] +id: ECO:0005609 +name: capillary assay evidence +def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by measuring the rate of accumulation of the cells into a capillary." [ECO:SN, PMID:4632978] +comment: To determine the presence of negative chemotaxis, a repellent is added to the organismal suspension (but not in the capillary) and the organisms that fled into the capillary are measured. +comment: To determine the presence of positive chemotaxis, an attractant is placed in the capillary and the presence of positive chemotaxis is determined based on the amount of organism that accumulated in the capillary. +is_a: ECO:0005021 ! chemotaxis assay evidence +created_by: snadendla +creation_date: 2016-03-31T16:45:29Z + +[Term] +id: ECO:0005610 +name: biological system reconstruction evidence based on homology evidence used in manual assertion +def: "A type of biological system reconstruction evidence based on homology evidence that is used in a manual assertion." [ECO:SN] +comment: Inference may be based on paralogy and/or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments in the same or another species. +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence +created_by: snadendla +creation_date: 2016-03-31T17:00:03Z + +[Term] +id: ECO:0005611 +name: inference from experimental data evidence +def: "A type of direct assay evidence used in manual assertion where phenotype or disease association is made with the gene because of involvement in the molecular mechanism of disease or involvement in a pathway known to contribute to a disease." [ECO:SN] +synonym: "IED" EXACT [] +synonym: "inferred from experimental data" EXACT [] +is_obsolete: true +created_by: snadendla +creation_date: 2016-04-12T16:55:16Z + +[Term] +id: ECO:0005612 +name: inference from phenotype manipulation evidence +def: "A type of mutant phenotype evidence used in manual assertion where variations or changes, such as mutations or abnormal levels of the product(s) of a single gene of interest, including non-mutational changes such as inhibition with antibodies or other inhibitors are determined." [ECO:SN] +comment: Changes can include any gene mutation/knockout, over-expression or ectopic expression of wild-type or mutant genes, anti-sense experiments, RNAi experiments, specific protein inhibitors. +synonym: "inferred from phenotype manipulation" EXACT [] +synonym: "IPM" EXACT [] +is_obsolete: true +created_by: snadendla +creation_date: 2016-04-12T17:09:43Z + +[Term] +id: ECO:0005613 +name: inference by association of genotype from phenotype +def: "A type of natural variation mutant evidence used in manual assertion where alleles are directly linked to phenotype and Mendelian diseases as a result of various experiments." [ECO:SN] +comment: Used for 1) polymorphism or segregation of genetic markers (SNPs, mutations, RFLPs, microsatellites); 2) polymorphism or segregation of physical markers (FISH, centromeric or heterochromatic regions, chromosomal banding patterns); 3) detection of polymorphisms in inbred stock. +synonym: "IAGP" EXACT [] +synonym: "inferred by association of genotype from phenotype" EXACT [] +is_a: ECO:0001237 ! natural variation mutant evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-04-12T17:24:00Z + +[Term] +id: ECO:0005614 +name: two-dimensional polyacrylamide gel electrophoresis evidence used in manual assertion +def: "A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in a manual assertion." [ECO:SN] +synonym: "2D-PAGE evidence" BROAD [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005503 ! two-dimensional polyacrylamide gel electrophoresis evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-05-06T11:03:08Z + +[Term] +id: ECO:0005615 +name: alkaline phosphatase reporter gene assay evidence used in manual assertion +def: "A type of alkaline phosphatase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "SEAP reporter assay" RELATED [] +synonym: "secreted embryonic alkaline phosphatase reporter assay" RELATED [] +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0001801 ! alkaline phosphatase reporter gene assay evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T11:05:59Z + +[Term] +id: ECO:0005616 +name: beta-galactosidase reporter gene assay evidence used in manual assertion +def: "A type of beta-galactosidase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "beta-gal reporter gene assay evidence" BROAD [] +synonym: "lacZ reporter gene assay" RELATED [] +synonym: "LacZ transcript localization evidence used in manual assertion" EXACT [] +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0001802 ! beta-galactosidase reporter gene assay evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T11:08:57Z + +[Term] +id: ECO:0005617 +name: chloramphenicol acetyltransferase reporter gene assay evidence used in manual assertion +def: "A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "CAT reporter gene assay evidence" BROAD [] +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0001803 ! chloramphenicol acetyltransferase reporter gene assay evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T11:11:07Z + +[Term] +id: ECO:0005618 +name: chromatin immunoprecipitation-chip evidence used in automatic assertion +def: "A type of chromatin immunoprecipitation-chip evidence that is used in an automatic assertion." [ECO:SN] +synonym: "ChIP-chip evidence" BROAD [] +synonym: "ChIP-on-chip evidence" BROAD [] +is_a: ECO:0000230 ! chromatin immunoprecipitation-chip evidence +is_a: ECO:0007786 ! chromatin immunoprecipitation evidence used in automatic assertion +created_by: snadendla +creation_date: 2016-05-06T11:17:04Z + +[Term] +id: ECO:0005619 +name: chromatin immunoprecipitation- exonuclease evidence used in automatic assertion +def: "A type of chromatin immunoprecipitation- exonuclease evidence that is used in an automatic assertion." [ECO:SN] +synonym: "ChIP-exo evidence" BROAD [] +is_a: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence +is_a: ECO:0007786 ! chromatin immunoprecipitation evidence used in automatic assertion +created_by: snadendla +creation_date: 2016-05-06T11:22:25Z + +[Term] +id: ECO:0005620 +name: chromatin immunoprecipitation-PCR evidence used in manual assertion +def: "A type of chromatin immunoprecipitation-PCR evidence that is used in a manual assertion." [ECO:SN] +synonym: "ChIP-PCR evidence" BROAD [] +is_a: ECO:0000227 ! chromatin immunoprecipitation-PCR evidence +is_a: ECO:0005644 ! immunoprecipitation evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T11:25:01Z + +[Term] +id: ECO:0005621 +name: chromatin immunoprecipitation-seq evidence used in automatic assertion +def: "A type of chromatin immunoprecipitation-seq evidence that is used in an automatic assertion." [ECO:SN] +synonym: "ChIP-SEQ evidence" BROAD [] +synonym: "ChIP-seq evidence" BROAD [] +is_a: ECO:0000229 ! chromatin immunoprecipitation-seq evidence +is_a: ECO:0007786 ! chromatin immunoprecipitation evidence used in automatic assertion +created_by: snadendla +creation_date: 2016-05-06T11:29:13Z + +[Term] +id: ECO:0005622 +name: comparative genomics motif search evidence used in manual assertion +def: "A type of comparative genomics motif search evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000257 ! motif similarity evidence used in manual assertion +is_a: ECO:0005534 ! comparative genomics motif search evidence +property_value: ECO:9000002 "ISM" xsd:string +created_by: snadendla +creation_date: 2016-05-06T14:49:36Z + +[Term] +id: ECO:0005623 +name: comparative genomics motif search evidence used in automatic assertion +def: "A type of comparative genomics motif search evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0000258 ! motif similarity evidence used in automatic assertion +is_a: ECO:0005534 ! comparative genomics motif search evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-05-06T14:52:21Z + +[Term] +id: ECO:0005624 +name: consensus search evidence used in manual assertion +def: "A type of consensus search evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000257 ! motif similarity evidence used in manual assertion +is_a: ECO:0005532 ! consensus search evidence +property_value: ECO:9000002 "ISM" xsd:string +created_by: snadendla +creation_date: 2016-05-06T14:54:53Z + +[Term] +id: ECO:0005625 +name: consensus search evidence used in automatic assertion +def: "A type of consensus search evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0000258 ! motif similarity evidence used in automatic assertion +is_a: ECO:0005532 ! consensus search evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-05-06T14:58:22Z + +[Term] +id: ECO:0005626 +name: copper-phenanthroline footprinting evidence used in manual assertion +def: "A type of copper-phenanthroline footprinting evidence that is used in a manual assertion." [ECO:SN] +synonym: "1,10-phenanthroline-copper footprinting evidence" BROAD [] +synonym: "OP-Cu Complex" RELATED [] +is_a: ECO:0001815 ! copper-phenanthroline footprinting evidence +is_a: ECO:0006083 ! nucleic acid binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T15:01:25Z + +[Term] +id: ECO:0005627 +name: DNA adenine methyltransferase identification evidence used in manual assertion +def: "A type of DNA adenine methyltransferase identification evidence that is used in a manual assertion." [ECO:SN] +synonym: "DamID evidence" BROAD [] +is_a: ECO:0001824 ! DNA adenine methyltransferase identification evidence +is_a: ECO:0006075 ! affinity evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T15:05:14Z + +[Term] +id: ECO:0005628 +name: DNA adenine methyltransferase identification evidence used in automatic assertion +def: "A type of DNA adenine methyltransferase identification evidence that is used in an automatic assertion." [ECO:SN] +synonym: "DamID evidence" BROAD [] +is_a: ECO:0001824 ! DNA adenine methyltransferase identification evidence +is_a: ECO:0007384 ! affinity evidence used in automatic assertion +created_by: snadendla +creation_date: 2016-05-06T15:06:46Z + +[Term] +id: ECO:0005629 +name: DNA affinity chromatography evidence used in manual assertion +def: "A type of DNA affinity chromatography evidence that is used in a manual assertion." [ECO:SN] +synonym: "DNA affinity purification evidence" BROAD [] +is_a: ECO:0001809 ! DNA affinity chromatography evidence +is_a: ECO:0007680 ! chromatography evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T15:08:40Z + +[Term] +id: ECO:0005630 +name: cDNA to DNA expression microarray evidence used in automatic assertion +def: "A type of cDNA to DNA expression microarray evidence that is used in an automatic assertion." [ECO:SN] +synonym: "DNA microarray evidence" BROAD [] +synonym: "RNA microarray evidence" BROAD [] +is_a: ECO:0000097 ! cDNA to DNA expression microarray evidence +is_a: ECO:0007301 ! expression microarray evidence used in automatic assertion +created_by: snadendla +creation_date: 2016-05-06T15:31:51Z + +[Term] +id: ECO:0005631 +name: DNAse footprinting evidence used in manual assertion +def: "A type of DNAse footprinting evidence that is used in a manual assertion." [ECO:SN] +synonym: "DNase protection evidence" BROAD [] +is_a: ECO:0001810 ! DNAse footprinting evidence +is_a: ECO:0006083 ! nucleic acid binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T15:35:41Z + +[Term] +id: ECO:0005632 +name: fluorescence anisotropy evidence used in manual assertion +def: "A type of fluorescence anisotropy evidence that is used in a manual assertion." [ECO:SN] +synonym: "FA evidence" BROAD [] +synonym: "fluorescence polarization" RELATED [] +synonym: "FP" RELATED [] +is_a: ECO:0001249 ! fluorescence evidence used in manual assertion +is_a: ECO:0001811 ! fluorescence anisotropy evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-05-06T15:45:40Z + +[Term] +id: ECO:0005633 +name: ferric uptake regulator titration assay evidence used in manual assertion +def: "A type of ferric uptake regulator titration assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "FURTA evidence" BROAD [] +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0001829 ! ferric uptake regulator titration assay evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T15:49:18Z + +[Term] +id: ECO:0005634 +name: genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion +def: "A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in a manual assertion." [ECO:SN] +synonym: "genomic SELEX evidence" BROAD [] +is_a: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence +is_a: ECO:0006088 ! systematic evolution of ligands by exponential amplification evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T16:41:30Z + +[Term] +id: ECO:0005635 +name: genomic systematic evolution of ligands by exponential amplification evidence used in automatic assertion +def: "A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion." [ECO:SN] +synonym: "genomic SELEX evidence" BROAD [] +is_a: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence +is_a: ECO:0007510 ! systematic evolution of ligands by exponential amplification evidence used in automatic assertion +created_by: snadendla +creation_date: 2016-05-06T16:43:03Z + +[Term] +id: ECO:0005636 +name: green fluorescent protein reporter gene assay evidence used in manual assertion +def: "A type of green fluorescent protein reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "GFP promoter fusion" RELATED [] +synonym: "GFP reporter gene assay evidence" BROAD [] +synonym: "green fluorescent protein promoter fusion" RELATED [] +synonym: "green fluorescent protein transcript localization evidence used in manual assertion" EXACT [] +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0001816 ! green fluorescent protein reporter gene assay evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T16:46:32Z + +[Term] +id: ECO:0005637 +name: green fluorescent protein reporter gene assay evidence used in automatic assertion +def: "A type of green fluorescent protein reporter gene assay evidence that is used in an automatic assertion." [ECO:SN] +synonym: "GFP promoter fusion" RELATED [] +synonym: "GFP reporter gene assay evidence" BROAD [] +synonym: "green fluorescent protein promoter fusion" RELATED [] +synonym: "green fluorescent protein transcript localization evidence used in automatic assertion" EXACT [] +is_a: ECO:0001816 ! green fluorescent protein reporter gene assay evidence +is_a: ECO:0007765 ! reporter gene assay evidence used in automatic assertion +created_by: snadendla +creation_date: 2016-05-06T16:48:29Z + +[Term] +id: ECO:0005638 +name: cell growth regulation assay evidence used in manual assertion +def: "A type of cell growth regulation assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "growth curve analysis" RELATED [] +is_a: ECO:0000095 ! cell growth regulation assay evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-05-06T16:52:15Z + +[Term] +id: ECO:0005639 +name: cell growth regulation assay evidence used in automatic assertion +def: "A type of cell growth regulation assay evidence that is used in an automatic assertion." [ECO:SN] +synonym: "growth curve analysis" RELATED [] +is_a: ECO:0000095 ! cell growth regulation assay evidence +is_a: ECO:0007778 ! cell growth assay evidence used in automatic assertion +created_by: snadendla +creation_date: 2016-05-06T16:53:28Z + +[Term] +id: ECO:0005640 +name: glutathione S-transferase pull-down assay evidence used in manual assertion +def: "A type of glutathione S-transferase pull-down assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "GST pull-down assay evidence" BROAD [] +is_a: ECO:0001817 ! glutathione S-transferase pull-down assay evidence +is_a: ECO:0006077 ! bait-prey hybrid interaction evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T16:56:39Z + +[Term] +id: ECO:0005641 +name: beta-glucuronidase reporter gene assay evidence used in manual assertion +def: "A type of beta-glucuronidase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "GUS reporter gene assay evidence" BROAD [] +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0001804 ! beta-glucuronidase reporter gene assay evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T16:58:29Z + +[Term] +id: ECO:0005642 +name: heteronuclear single quantum coherence spectroscopy evidence used in manual assertion +def: "A type of heteronuclear single quantum coherence spectroscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "HSQC evidence" BROAD [] +is_a: ECO:0001813 ! heteronuclear single quantum coherence spectroscopy evidence +is_a: ECO:0006165 ! nuclear magnetic resonance spectroscopy evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:10:13Z + +[Term] +id: ECO:0005643 +name: hydroxyl-radical footprinting evidence used in manual assertion +def: "A type of hydroxyl-radical footprinting evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001818 ! hydroxyl-radical footprinting evidence +is_a: ECO:0006083 ! nucleic acid binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:14:48Z + +[Term] +id: ECO:0005644 +name: immunoprecipitation evidence used in manual assertion +def: "A type of immunoprecipitation evidence that is used in a manual assertion." [ECO:SN] +synonym: "immunoprecipitation" BROAD [] +is_a: ECO:0000085 ! immunoprecipitation evidence +is_a: ECO:0006076 ! protein binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:20:49Z + +[Term] +id: ECO:0005645 +name: interferometric reflectance imaging sensor evidence used in manual assertion +def: "A type of interferometric reflectance imaging sensor evidence that is used in a manual assertion." [ECO:SN] +synonym: "IRIS evidence" BROAD [] +synonym: "spectral reflectance imaging biosensor evidence" BROAD [] +synonym: "SRIB evidence" BROAD [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0005520 ! interferometric reflectance imaging sensor evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:27:58Z + +[Term] +id: ECO:0005646 +name: interferometric reflectance imaging sensor evidence used in automatic assertion +def: "A type of interferometric reflectance imaging sensor evidence that is used in an automatic assertion." [ECO:SN] +synonym: "IRIS evidence" BROAD [] +synonym: "spectral reflectance imaging biosensor evidence" BROAD [] +synonym: "SRIB evidence" BROAD [] +is_a: ECO:0005520 ! interferometric reflectance imaging sensor evidence +is_a: ECO:0007816 ! molecule detection assay evidence used in automatic assertion +created_by: snadendla +creation_date: 2016-05-06T23:29:23Z + +[Term] +id: ECO:0005647 +name: isothermal titration calorimetry evidence used in manual assertion +def: "A type of isothermal titration calorimetry evidence that is used in a manual assertion." [ECO:SN] +synonym: "ITC evidence" BROAD [] +is_a: ECO:0001825 ! isothermal titration calorimetry evidence +is_a: ECO:0006075 ! affinity evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:36:26Z + +[Term] +id: ECO:0005648 +name: luciferase reporter gene assay evidence used in manual assertion +def: "A type of luciferase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0001805 ! luciferase reporter gene assay evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:44:52Z + +[Term] +id: ECO:0005649 +name: machine learning prediction of motif instance evidence used in manual assertion +def: "A type of machine learning prediction of motif instance evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000255 ! match to sequence model evidence used in manual assertion +is_a: ECO:0005535 ! machine learning prediction of motif instance evidence +property_value: ECO:9000002 "ISM" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:51:30Z + +[Term] +id: ECO:0005650 +name: machine learning prediction of motif instance evidence used in automatic assertion +def: "A type of machine learning prediction of motif instance evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0000256 ! match to sequence model evidence used in automatic assertion +is_a: ECO:0005535 ! machine learning prediction of motif instance evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:54:00Z + +[Term] +id: ECO:0005651 +name: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in automatic assertion +def: "A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in an automatic assertion." [ECO:SN] +synonym: "MALDI-TOF mass spectrometry evidence" BROAD [] +synonym: "MALDI-TOF-MS evidence" BROAD [] +is_a: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence +is_a: ECO:0007819 ! protein detection assay evidence used in automatic assertion +created_by: snadendla +creation_date: 2016-05-06T23:57:30Z + +[Term] +id: ECO:0005652 +name: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in manual assertion +def: "A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in a manual assertion." [ECO:SN] +synonym: "Methidiumpropyl-EDTA Fe(II) footprinting evidence" BROAD [] +synonym: "MPE Fe(II) footprinting" RELATED [] +synonym: "MPE-EDTA Fe(II) footprinting evidence" BROAD [] +is_a: ECO:0001814 ! methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence +is_a: ECO:0006083 ! nucleic acid binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-09T14:42:28Z + +[Term] +id: ECO:0005653 +name: northern assay evidence used in manual assertion +def: "A type of northern assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "northern assay evidence" BROAD [] +synonym: "RNA blot evidence" BROAD [] +synonym: "transcript levels (e.g. Northerns)" RELATED [] +is_a: ECO:0000106 ! northern blot evidence +is_a: ECO:0000291 ! transcript expression evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T14:48:03Z + +[Term] +id: ECO:0005654 +name: phylogenetic footprinting evidence used in manual assertion +def: "A type of phylogenetic footprinting evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005533 ! phylogenetic footprinting evidence +is_a: ECO:0005558 ! motif discovery evidence used in manual assertion +property_value: ECO:9000002 "ISA" xsd:string +created_by: snadendla +creation_date: 2016-05-09T14:50:52Z + +[Term] +id: ECO:0005655 +name: phylogenetic footprinting evidence used in automatic assertion +def: "A type of phylogenetic footprinting evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0005533 ! phylogenetic footprinting evidence +is_a: ECO:0005559 ! motif discovery evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-05-09T14:51:55Z + +[Term] +id: ECO:0005656 +name: methylation interference footprinting evidence used in manual assertion +def: "A type of methylation interference footprinting evidence that is used in a manual assertion." [ECO:SN] +synonym: "premethylation interference footprinting evidence used in manual assertion" EXACT [] +is_a: ECO:0001827 ! methylation interference footprinting evidence +is_a: ECO:0006083 ! nucleic acid binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-09T14:55:25Z + +[Term] +id: ECO:0005657 +name: primer extension assay evidence used in manual assertion +def: "A type of primer extension assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000291 ! transcript expression evidence used in manual assertion +is_a: ECO:0001819 ! primer extension assay evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T14:58:38Z + +[Term] +id: ECO:0005658 +name: position-specific scoring matrix motif search evidence used in manual assertion +def: "A type of position-specific scoring matrix motif search evidence that is used in a manual assertion." [ECO:SN] +synonym: "PSSM motif search evidence" BROAD [] +is_a: ECO:0000257 ! motif similarity evidence used in manual assertion +is_a: ECO:0005536 ! position-specific scoring matrix motif search evidence +property_value: ECO:9000002 "ISM" xsd:string +created_by: snadendla +creation_date: 2016-05-09T15:01:31Z + +[Term] +id: ECO:0005659 +name: position-specific scoring matrix motif search evidence used in automatic assertion +def: "A type of position-specific scoring matrix motif search evidence that is used in an automatic assertion." [ECO:SN] +synonym: "PSSM motif search evidence" BROAD [] +is_a: ECO:0000258 ! motif similarity evidence used in automatic assertion +is_a: ECO:0005536 ! position-specific scoring matrix motif search evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-05-09T15:02:31Z + +[Term] +id: ECO:0005660 +name: quantitative polymerase chain reaction evidence used in manual assertion +def: "A type of quantitative polymerase chain reaction evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000231 ! quantitative polymerase chain reaction evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T15:09:01Z + +[Term] +id: ECO:0005661 +name: rapid amplification of cDNA ends polymerase chain reaction evidence used in manual assertion +def: "A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in a manual assertion." [ECO:SN] +synonym: "RACE PCR evidence" BROAD [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001820 ! rapid amplification of cDNA ends polymerase chain reaction evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T15:19:20Z + +[Term] +id: ECO:0005662 +name: regular expression motif search evidence used in manual assertion +def: "A type of regular expression motif search evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000257 ! motif similarity evidence used in manual assertion +is_a: ECO:0005505 ! regular expression motif search evidence +property_value: ECO:9000002 "ISM" xsd:string +created_by: snadendla +creation_date: 2016-05-09T16:13:55Z + +[Term] +id: ECO:0005663 +name: regular expression motif search evidence used in automatic assertion +def: "A type of regular expression motif search evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0000258 ! motif similarity evidence used in automatic assertion +is_a: ECO:0005505 ! regular expression motif search evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-05-09T16:15:03Z + +[Term] +id: ECO:0005664 +name: RNA sequencing assay evidence used in manual assertion +comment: Use ECO:0006068 (RNA-seq evidence used in manual assertion) in place of this term. +is_obsolete: true +created_by: snadendla +creation_date: 2016-05-09T16:18:35Z + +[Term] +id: ECO:0005665 +name: RNA sequencing assay evidence used in automatic assertion +comment: Use ECO:0006069 (RNA-seq evidence used in automatic assertion) in place of this term. +is_obsolete: true +created_by: snadendla +creation_date: 2016-05-09T16:19:38Z + +[Term] +id: ECO:0005666 +name: S1 nuclease protection assay evidence used in manual assertion +def: "A type of S1 nuclease protection assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001240 ! nuclease protection assay evidence used in manual assertion +is_a: ECO:0005521 ! S1 nuclease protection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T16:22:58Z + +[Term] +id: ECO:0005667 +name: site-directed mutagenesis phenotypic evidence used in manual assertion +def: "A type of site-directed phenotypic mutagenesis evidence that is used in a manual assertion." [ECO:SN] +synonym: "site directed mutagenesis evidence" BROAD [] +synonym: "site-directed mutagenesis evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005528 ! site-directed mutagenesis phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T16:29:13Z + +[Term] +id: ECO:0005668 +name: survival rate analysis evidence used in manual assertion +def: "A type of survival rate analysis evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001225 ! knockout evidence used in manual assertion +is_a: ECO:0001822 ! survival rate analysis evidence +property_value: ECO:9000002 "IMP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T16:31:08Z + +[Term] +id: ECO:0005669 +name: ultraviolet light footprinting evidence used in manual assertion +def: "A type of ultraviolet light footprinting evidence that is used in a manual assertion." [ECO:SN] +synonym: "UV footprinting" RELATED [] +is_a: ECO:0001826 ! ultraviolet light footprinting evidence +is_a: ECO:0006083 ! nucleic acid binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-09T16:33:44Z + +[Term] +id: ECO:0005670 +name: x-ray crystallography evidence used in manual assertion +def: "A type of x-ray crystallography evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001171 ! crystallography evidence used in manual assertion +is_a: ECO:0001823 ! x-ray crystallography evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-10T11:53:00Z + +[Term] +id: ECO:0005671 +name: x-ray crystallography evidence used in automatic assertion +def: "A type of x-ray crystallography evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0001823 ! x-ray crystallography evidence +is_a: ECO:0007374 ! crystallography evidence used in automatic assertion +created_by: snadendla +creation_date: 2016-05-10T11:54:03Z + +[Term] +id: ECO:0005672 +name: xylE reporter gene assay evidence used in manual assertion +def: "A type of xylE reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0005537 ! xylE reporter gene assay evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-10T11:57:15Z + +[Term] +id: ECO:0005673 +name: ad-hoc qualitative phenotype observation evidence +def: "A type of experimental phenotypic evidence where a non-standard trait (e.g. natural competence) is assessed qualitatively (e.g. presence/absence) and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor." [ECO:SN, PMID:15150239] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2016-05-10T12:57:41Z + +[Term] +id: ECO:0005674 +name: ad-hoc qualitative phenotype observation evidence used in manual assertion +def: "A type of ad-hoc qualitative phenotype observation that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005673 ! ad-hoc qualitative phenotype observation evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-10T14:28:47Z + +[Term] +id: ECO:0005675 +name: ad-hoc quantitative phenotype observation evidence +def: "A type of experimental phenotypic evidence where a quantifiable phenotypic trait (e.g. glucose intake) is assessed in a quantitative manner after induction of the regulatory mechanism and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor." [ECO:SN, PMID:15150239] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2016-05-10T14:30:50Z + +[Term] +id: ECO:0005676 +name: ad-hoc quantitative phenotype observation evidence used in manual assertion +def: "A type of ad-hoc quantitative phenotype observation evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005675 ! ad-hoc quantitative phenotype observation evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-10T14:34:50Z + +[Term] +id: ECO:0005800 +name: dilution assay evidence +def: "A type of direct assay evidence where the activity of a solution is determined by exposing an indicator culture or organism to a series of dilutions and determining the lowest concentration that elicits a biological response." [http://www.sciencedirect.com/science/article/pii/016770129400068I] +comment: For chemicals, bacteriocins, or phage, the indicator culture is typically present in a soft-agar layer on top of nutrient agar and a small quantity of each dilution is spotted onto the soft agar overlay. +synonym: "critical dilution assay" EXACT [] +synonym: "spot assay" EXACT [] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0005801 +name: enzymatic activity assay evidence used in manual assertion +def: "A type of enzyme assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "enzyme assays" BROAD [] +is_a: ECO:0000005 ! enzymatic activity assay evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string + +[Term] +id: ECO:0005802 +name: cell transfection experiment evidence used in manual assertion +def: "A type of cell transfection experiment evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0005606 ! cell transfection experiment evidence +property_value: ECO:9000002 "EXP" xsd:string + +[Term] +id: ECO:0005803 +name: motility assay evidence +def: "A type of direct assay evidence where the motility of a cell or cells is determined." [ECO:SN] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000112 "A motility assay at 37 degrees C revealed a non-motile phenotype for all strains, as expected (data not shown)." xsd:string {xref="PMID:20830609"} + +[Term] +id: ECO:0005804 +name: immunofluorescence evidence used in manual assertion +def: "A type of immunofluorescence evidence that is used in a manual assertion." [ECO:SN] +synonym: "immunofluorescence" BROAD [] +is_a: ECO:0000007 ! immunofluorescence evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string + +[Term] +id: ECO:0005805 +name: yeast 2-hybrid evidence used in manual assertion +def: "A type of yeast 2-hybrid evidence that is used in a manual assertion." [ECO:SN] +synonym: "yeast two-hybrid assay" BROAD [] +is_a: ECO:0000068 ! yeast 2-hybrid evidence +is_a: ECO:0006077 ! bait-prey hybrid interaction evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string + +[Term] +id: ECO:0006001 +name: Cya fusion reporter assay evidence +def: "A type of evidence derived from exploiting the CyaA (calmodulin-activated adenylate cyclase toxin) secreted by Bordatella pertussis to synthesize cAMP and alter cellular physiology in a host cell for various biological applications." [PMID:10217833, PMID:14702323] +is_a: ECO:0000017 ! ectopic expression evidence +is_a: ECO:0000049 ! reporter gene assay evidence +created_by: rctauber +creation_date: 2016-06-15T08:42:16Z + +[Term] +id: ECO:0006002 +name: Cya fusion reporter assay evidence used in manual assertion +def: "A type of Cya fusion reporter assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0001273 ! ectopic expression evidence used in manual assertion +is_a: ECO:0006001 ! Cya fusion reporter assay evidence +property_value: ECO:9000002 "IEP" xsd:string +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: rctauber +creation_date: 2010-06-15T08:44:10Z + +[Term] +id: ECO:0006003 +name: electron microscopy evidence used in manual assertion +def: "A type of electron microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001232 ! microscopy evidence used in manual assertion +is_a: ECO:0005033 ! electron microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-10-12T12:32:29Z + +[Term] +id: ECO:0006004 +name: super-resolution microscopy evidence used in manual assertion +def: "A type of super-resolution microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001232 ! microscopy evidence used in manual assertion +is_a: ECO:0005578 ! super-resolution microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-10-12T12:32:29Z + +[Term] +id: ECO:0006005 +name: fractionation evidence used in manual assertion +def: "A type of fractionation evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000100 ! fractionation evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-10-12T12:32:29Z + +[Term] +id: ECO:0006006 +name: electrophysiology assay evidence used in manual assertion +def: "A type of electrophysiology assay evidence that is used in a manual assertion." [] {comment="ECO:RCT"} +is_a: ECO:0000164 ! electrophysiology assay evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-10-12T12:32:29Z + +[Term] +id: ECO:0006007 +name: chromatin immunoprecipitation-chip evidence used in manual assertion +def: "A type of chromatin immunoprecipitation-chip evidence that is used in a manual assertion." [ECO:RCT] +synonym: "ChIP-chip evidence" BROAD [] +synonym: "ChIP-on-chip evidence" BROAD [] +is_a: ECO:0000230 ! chromatin immunoprecipitation-chip evidence +is_a: ECO:0005644 ! immunoprecipitation evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2016-10-21T11:55:53Z + +[Term] +id: ECO:0006008 +name: chromatin immunoprecipitation- exonuclease evidence used in manual assertion +def: "A type of chromatin immunoprecipitation- exonuclease evidence that is used in a manual assertion." [ECO:RCT] +synonym: "ChIP-exo" BROAD [] +is_a: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence +is_a: ECO:0005644 ! immunoprecipitation evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2016-10-21T11:55:53Z + +[Term] +id: ECO:0006009 +name: chromatin immunoprecipitation-seq evidence used in manual assertion +def: "A type of chromatin immunoprecipitation-seq evidence that is used in a manual assertion." [ECO:RCT] +synonym: "ChIP-SEQ evidence" BROAD [] +synonym: "ChIP-seq evidence" BROAD [] +is_a: ECO:0000229 ! chromatin immunoprecipitation-seq evidence +is_a: ECO:0005644 ! immunoprecipitation evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2016-10-21T11:55:53Z + +[Term] +id: ECO:0006010 +name: mRNA interactome capture evidence +def: "A type of nucleic acid binding evidence in which RNA-binding proteins are identified through cross-linking RNA and proteins by UV light, capturing RNA-protein complexes on oligo(dT) beads, and finally identifying by mass spectrometry." [PMID:27729395] +synonym: "ultraviolet light cross-linking RNA binding evidence" EXACT [] +is_a: ECO:0000136 ! nucleic acid binding evidence +created_by: rctauber +creation_date: 2016-10-21T12:28:17Z + +[Term] +id: ECO:0006011 +name: mRNA interactome capture evidence used in manual assertion +def: "A type of mRNA interactome capture evidence that is used in a manual assertion." [ECO:RCT] +synonym: "ultraviolet light cross-linking RNA binding evidence" BROAD [] +is_a: ECO:0006010 ! mRNA interactome capture evidence +is_a: ECO:0006083 ! nucleic acid binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2016-10-21T12:28:17Z + +[Term] +id: ECO:0006012 +name: patch-clamp recording evidence +def: "A type of electrophysiology assay evidence in which a glass micropipette is sealed to the surface of a cell membrane (patch) to study ion channels." [ECO:RCT] +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: rctauber +creation_date: 2016-11-28T11:44:05Z + +[Term] +id: ECO:0006013 +name: patch-clamp recording evidence used in manual assertion +def: "A type of patch-clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +is_a: ECO:0006012 ! patch-clamp recording evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-11-28T11:44:05Z + +[Term] +id: ECO:0006014 +name: whole-cell patch-clamp recording evidence +def: "A type of patch-clamp recording evidence in which a glass micropipette filled with a prepared solution is used to form a seal with the cell membrane followed by rupture of membrane to provide accurate and high resolution electrical property measurements of the whole cell." [PMID:27341060] +is_a: ECO:0006012 ! patch-clamp recording evidence +created_by: rctauber +creation_date: 2016-11-28T11:44:05Z + +[Term] +id: ECO:0006015 +name: whole-cell patch-clamp recording evidence used in manual assertion +def: "A type of whole-cell patch-clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006013 ! patch-clamp recording evidence used in manual assertion +is_a: ECO:0006014 ! whole-cell patch-clamp recording evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-11-28T11:44:05Z + +[Term] +id: ECO:0006016 +name: author statement from published clinical study +def: "A type of traceable author statement that is based on a publication about a clinical study." [] +synonym: "published clinical study evidence" EXACT [] +synonym: "traceable author statement from published clinical study" RELATED [] +is_a: ECO:0000033 ! author statement supported by traceable reference +created_by: rctauber +creation_date: 2016-11-30T09:44:47Z + +[Term] +id: ECO:0006017 +name: author statement from published clinical study used in manual assertion +def: "A type of author statement from published clinical study that is used in a manual assertion." [] +comment: Created and used by the Human Phenotype Ontology (HPO) Annotations group. Here it is used when evidence for an HPO annotation is information extracted from articles in the medical literature. +synonym: "PCS" EXACT [HPO:PCS] +synonym: "published clinical study evidence" BROAD [] +is_a: ECO:0000304 ! author statement supported by traceable reference used in manual assertion +is_a: ECO:0006016 ! author statement from published clinical study +property_value: ECO:9000002 "TAS" xsd:string +property_value: seeAlso http://human-phenotype-ontology.github.io/documentation.html#annot xsd:string +created_by: rctauber +creation_date: 2016-11-30T09:44:47Z + +[Term] +id: ECO:0006018 +name: inference based on individual clinical experience +def: "A type of curator inference that is based on the individual clinical experience of a clinician" [] +synonym: "individual clinical experience evidence" EXACT [] +is_a: ECO:0000205 ! curator inference +created_by: rctauber +creation_date: 2016-11-30T09:44:47Z + +[Term] +id: ECO:0006019 +name: inference based on individual clinical experience used in manual assertion +def: "A type of inference based on individual clinical experience that is used in a manual assertion." [] +comment: Created and used by the Human Phenotype Ontology (HPO) Annotations group. Here it is used for annotating disorders with a limited amount of published data, and is accompanied by a reference to the individual or center performing the annotation. +synonym: "ICE" EXACT [HPO:ICE] +synonym: "individual clinical experience evidence" BROAD [] +is_a: ECO:0000305 ! curator inference used in manual assertion +is_a: ECO:0006018 ! inference based on individual clinical experience +property_value: ECO:9000002 "IC" xsd:string +property_value: seeAlso http://human-phenotype-ontology.github.io/documentation.html#annot xsd:string +created_by: rctauber +creation_date: 2016-11-30T09:44:47Z + +[Term] +id: ECO:0006020 +name: biofilm formation assay evidence +def: "A type of cell proliferation assay evidence in which biofilm growth is monitored and detected from attachment to development." [PMID:10547784] +synonym: "biofilm assay evidence" EXACT [] +is_a: ECO:0005007 ! cell proliferation assay evidence +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006021 +name: biofilm formation assay evidence used in manual assertion +def: "A type of biofilm formation assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0006020 ! biofilm formation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006022 +name: microtiter plate biofilm assay evidence +def: "A type of biofilm formation assay evidence in which microtiter dishes or tubes are inoculated and incubated to promote biofilm formation, and then biofilms are detected by staining with crystal violet or safranin to observe phenotypes." [PMID:10547784] +synonym: "96-well biofilm assay evidence" EXACT [] +is_a: ECO:0006020 ! biofilm formation assay evidence +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006023 +name: microtiter plate biofilm assay evidence used in manual assertion +def: "A type of microtiter plate biofilm assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006021 ! biofilm formation assay evidence used in manual assertion +is_a: ECO:0006022 ! microtiter plate biofilm assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006024 +name: air-liquid interface assay evidence +def: "A type of biofilm formation assay evidence in which biofilm formation is analyzed, without staining, over 4 to 48 hours by growth on a tilted multiwell plate." [PMID:18770545] +comment: Tilting of the plate positions the air-liquid interface on a clear portion to improve visability. +synonym: "ALI biofilm assay evidence" EXACT [] +is_a: ECO:0006020 ! biofilm formation assay evidence +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006025 +name: air-liquid interface assay evidence used in manual assertion +def: "A type of air-liquid interface assay evidence that is used in a manual assertion." [ECO:RCT] +synonym: "ALI biofilm assay evidence" BROAD [] +is_a: ECO:0006021 ! biofilm formation assay evidence used in manual assertion +is_a: ECO:0006024 ! air-liquid interface assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006026 +name: colony biofilm assay evidence +def: "A type of biofilm formation assay evidence in which a colony is grown on a semipermeable membrane on an agar plate. The plate serves to supply nutrients and the semipermeable membrane can be relocated to fresh plates." [PMID:18770545] +comment: The plates commonly contain different carbon sources or antibiotic treatments to observe properties of the cells. +is_a: ECO:0006020 ! biofilm formation assay evidence +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006027 +name: colony biofilm assay evidence used in manual assertion +def: "A type of colony biofilm assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006021 ! biofilm formation assay evidence used in manual assertion +is_a: ECO:0006026 ! colony biofilm assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006028 +name: Kadouri drip-fed biofilm assay evidence +def: "A type of biofilm formation assay evidence in which mature bacterial biofilms are grown in a multiwell plate that has a growth medium continually pumped through the wells while waste is continually pumped out." [PMID:18770545] +is_a: ECO:0006020 ! biofilm formation assay evidence +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006029 +name: Kadouri drip-fed biofilm assay evidence used in manual assertion +def: "A type of Kadouri drip-fed biofilm assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006021 ! biofilm formation assay evidence used in manual assertion +is_a: ECO:0006028 ! Kadouri drip-fed biofilm assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006030 +name: co-immunoprecipitation evidence used in manual assertion +def: "A type of co-immunoprecipitation evidence that is used in a manual assertion." [ECO:RCT] +synonym: "co-immunoprecipitation" BROAD [] +is_a: ECO:0000070 ! co-immunoprecipitation evidence +is_a: ECO:0005644 ! immunoprecipitation evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2016-01-13T11:32:26Z + +[Term] +id: ECO:0006031 +name: immunolocalization evidence used in manual assertion +def: "A type of immunolocalization evidence that is used in a manual assertion." [ECO:RCT] +synonym: "immunolocalization" BROAD [] +is_a: ECO:0000087 ! immunolocalization evidence +is_a: ECO:0001232 ! microscopy evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-01-23T07:54:37Z + +[Term] +id: ECO:0006032 +name: optogenetic evidence +def: "A type of experimental phenotypic evidence arising from experiment in which neuronal activity is manipulated using genetically encoded, optically activated neuronal actuators." [GOC:DOS, PMID:17035522] +synonym: "optogenetic actuator evidence" EXACT [] +is_a: ECO:0000059 ! experimental phenotypic evidence +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006033 +name: optogenetic evidence used in manual assertion +def: "A type of optogenetic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "optogenetic actuator evidence" BROAD [] +is_a: ECO:0006032 ! optogenetic evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006034 +name: fluorescent sensor evidence +def: "A type of fluorescence evidence that is based on direct, quantitative measurement of some cellular property using a fluorescent sensor." [GOC:DOS] +comment: Examples include sensors for ion concentration and potential difference across a membrane. +is_a: ECO:0001115 ! fluorescence evidence +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006035 +name: fluorescent sensor evidence used in manual assertion +def: "A type of fluorescent sensor evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001249 ! fluorescence evidence used in manual assertion +is_a: ECO:0006034 ! fluorescent sensor evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006036 +name: genetically encoded fluorescent sensor evidence +def: "A type of fluorescent sensor evidence that is based on direct, quantitative measurement of some cellular property using a genetically encoded fluorescent sensor." [GOC:DOS] +synonym: "optogenetic sensor evidence" EXACT [] +is_a: ECO:0006034 ! fluorescent sensor evidence +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006037 +name: genetically encoded fluorescent sensor evidence used in manual assertion +def: "A type of genetically encoded fluorescent sensor evidence that is used in a manual assertion." [ECO:RCT] +synonym: "optogenetic sensor evidence" BROAD [] +is_a: ECO:0006035 ! fluorescent sensor evidence used in manual assertion +is_a: ECO:0006036 ! genetically encoded fluorescent sensor evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006038 +name: genetically encoded fluorescent electrophysiology assay evidence +def: "A type of fluorescent sensor evidence and electrophysiology assay evidence where the electrical properties of cells or tissues are studied using fluorescent sensors." [GOC:DOS] +comment: Examples include genetically encoded sensors that detect potential difference across plasma membrane. +synonym: "electrophysiology - optical assay evidence" EXACT [] +is_a: ECO:0000164 ! electrophysiology assay evidence +is_a: ECO:0006034 ! fluorescent sensor evidence +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006039 +name: genetically encoded fluorescent electrophysiology assay evidence used in manual assertion +def: "A type of genetically encoded fluorescent electrophysiology assay evidence that is used in a manual assertion." [ECO:RCT] +synonym: "electrophysiology - optical assay evidence" BROAD [] +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +is_a: ECO:0006035 ! fluorescent sensor evidence used in manual assertion +is_a: ECO:0006038 ! genetically encoded fluorescent electrophysiology assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006040 +name: genetically encoded fluorescent ion concentration sensor assay evidence +def: "A type of genetically encoded fluorescent electrophysiology assay evidence that is based on direct, quantitative measurement of ion concentration using a genetically encoded fluorescent sensor." [GOC:DOS] +comment: Examples include the genetically encoded calcium indicator GCaMP. +is_a: ECO:0006038 ! genetically encoded fluorescent electrophysiology assay evidence +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006041 +name: genetically encoded fluorescent ion concentration sensor assay evidence used in manual assertion +def: "A type of genetically encoded fluorescent ion concentration sensor assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006039 ! genetically encoded fluorescent electrophysiology assay evidence used in manual assertion +is_a: ECO:0006040 ! genetically encoded fluorescent ion concentration sensor assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006042 +name: cell fractionation evidence used in manual assertion +def: "A type of cell fractionation evidence that is used in a manual assertion." [ECO:RCT] +synonym: "cell fractionation" BROAD [] +is_a: ECO:0000004 ! cell fractionation evidence +is_a: ECO:0006005 ! fractionation evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006043 +name: extracellular recording evidence +def: "A type of electrophysiology assay evidence resulting from the use of electrodes to measure in vivo electrical activity coming from adjacent neurons." [url:http\://www.nature.com/subjects/extracellular-recording] +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006044 +name: extracellular recording evidence used in manual assertion +def: "A type of extracellular recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +is_a: ECO:0006043 ! extracellular recording evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006045 +name: single-unit extracellular recording evidence +def: "A type of extracellular recording evidence in which an extracellular microelectrode is used to measure the electrical activity of a single neuron." [doi:10.1385/0-89603-185-3\:1] +is_a: ECO:0006043 ! extracellular recording evidence +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006046 +name: single-unit extracellular recording evidence used in manual assertion +def: "A type of single-unit extracellular recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006044 ! extracellular recording evidence used in manual assertion +is_a: ECO:0006045 ! single-unit extracellular recording evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006047 +name: field potential recording evidence +def: "A type of extracellular recording evidence in which electrical activity is measured in either tissue or at a cellular level with microelectrodes." [ECO:RCT] +synonym: "local field potential recording evidence" NARROW [] +is_a: ECO:0006043 ! extracellular recording evidence +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006048 +name: field potential recording evidence used in manual assertion +def: "A type of field potential recording evidence that is used in a manual assertion." [ECO:RCT] +synonym: "local field potential recording evidence" RELATED [] +is_a: ECO:0006044 ! extracellular recording evidence used in manual assertion +is_a: ECO:0006047 ! field potential recording evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006049 +name: genetic transformation evidence used in manual assertion +def: "A type of genetic transformation evidence that is used in a manual assertion" [ECO:RCT] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005027 ! genetic transformation evidence +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2017-02-21T18:08:58Z + +[Term] +id: ECO:0006050 +name: anti-sense experiment evidence used in manual assertion +def: "A type of anti-sense experiment evidence that is used in a manual assertion." [ECO:RCT] +synonym: "anti-sense experiments" BROAD [] +is_a: ECO:0000018 ! anti-sense experiment evidence +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2017-02-21T18:08:58Z + +[Term] +id: ECO:0006051 +name: morpholino experiment evidence used in manual assertion +def: "A type of morpholino experiment evidence that is used in a manual assertion." [ECO:RCT] +synonym: "anti-sense evidence" BROAD [] +is_a: ECO:0000292 ! morpholino experiment evidence +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2017-02-21T18:08:58Z + +[Term] +id: ECO:0006052 +name: RNAi evidence used in manual assertion +def: "A type of RNAi evidence that is used in a manual assertion." [ECO:RCT] +synonym: "RNAi experiment" BROAD [] +is_a: ECO:0000019 ! RNAi evidence +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2017-02-21T18:08:58Z + +[Term] +id: ECO:0006053 +name: pharmacological assay evidence +def: "A type of experimental phenotypic evidence that arises from assaying the response of a cell, tissue, organ or organism following exposure to a receptor agonist or antagonist." [GOC:DOS] +is_a: ECO:0000059 ! experimental phenotypic evidence +created_by: dosumis +creation_date: 2017-03-07T08:51:02Z + +[Term] +id: ECO:0006054 +name: pharmacological assay evidence used in manual assertion +def: "A type of experimental phenotypic evidence used in a manual assertion that arises from assaying the response of a cell, tissue, organ or organism following exposure to a receptor agonist or antagonist." [GOC:DOS] +is_a: ECO:0006053 ! pharmacological assay evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2017-03-07T08:51:02Z + +[Term] +id: ECO:0006055 +name: high throughput evidence +def: "A type of evidence where data generation is automated with equipment to allow for assaying samples or molecules in parallel." [ECO:MCC] +comment: Some relevant articles include: PMID: 23340846, doi:10.1038/nmeth0607-523 +synonym: "high throughput cell biology evidence" NARROW [] +synonym: "high throughput screening evidence" NARROW [] +synonym: "high-throughput evidence" EXACT [] +synonym: "HT evidence" EXACT [] +is_a: ECO:0000000 ! evidence +created_by: mchibucos +creation_date: 2017-03-28T14:48:39Z + +[Term] +id: ECO:0006056 +name: high throughput evidence used in manual assertion +def: "A type of evidence that is used in a manual assertion where data generation is automated with equipment to allow for assaying large numbers of samples or molecules in parallel." [ECO:RCT] +synonym: "high throughput cell biology evidence" RELATED [] +synonym: "high throughput screening evidence" RELATED [] +synonym: "high-throughput evidence" BROAD [] +synonym: "HT evidence" BROAD [] +synonym: "HTP" EXACT [GOECO:HTP] +synonym: "inferred from high throughput experiment" EXACT [GOECO:HTP] +xref: GOECO:HTP "inferred from high throughput experiment" +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0006055 ! high throughput evidence +property_value: ECO:9000002 "HTP" xsd:string {comment="Default"} +created_by: rctauber +creation_date: 2017-03-28T14:48:39Z + +[Term] +id: ECO:0006057 +name: high throughput evidence used in automatic assertion +def: "A type of evidence that is used in an automatic assertion where data generation is automated with equipment to allow for assaying samples or molecules in parallel." [ECO:RCT] +synonym: "high throughput cell biology evidence" RELATED [] +synonym: "high throughput screening evidence" RELATED [] +synonym: "high-throughput evidence" BROAD [] +synonym: "HT evidence" BROAD [] +is_a: ECO:0000501 ! evidence used in automatic assertion +is_a: ECO:0006055 ! high throughput evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2017-03-28T14:48:39Z + +[Term] +id: ECO:0006058 +name: high throughput cell biology evidence +def: "A type of high throughput evidence where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel." [ECO:MCC] +comment: HT methodologies may incorporate techniques from optics, chemistry, biology or image analysis (https://en.wikipedia.org/wiki/High_throughput_biology) +synonym: "omics experiment" RELATED [] +is_a: ECO:0006055 ! high throughput evidence +created_by: mchibucos +creation_date: 2017-03-28T14:48:39Z + +[Term] +id: ECO:0006059 +name: high throughput cell biology evidence used in manual assertion +def: "A type of high throughput evidence used in a manual assertion where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel." [ECO:RCT] +synonym: "omics experiment" RELATED [] +is_a: ECO:0006056 ! high throughput evidence used in manual assertion +is_a: ECO:0006058 ! high throughput cell biology evidence +property_value: ECO:9000002 "HTP" xsd:string +created_by: rctauber +creation_date: 2017-03-28T14:48:39Z + +[Term] +id: ECO:0006060 +name: high throughput cell biology evidence used in automatic assertion +def: "A type of high throughput evidence used in an automatic assertion where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel." [ECO:RCT] +synonym: "omics experiment" RELATED [] +is_a: ECO:0006057 ! high throughput evidence used in automatic assertion +is_a: ECO:0006058 ! high throughput cell biology evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2017-03-28T14:48:39Z + +[Term] +id: ECO:0006061 +name: immunofluorescence wide-field microscopy evidence used in manual assertion +def: "A type of wide-field fluorescence microscopy evidence used in a manual assertion where fluorescently tagged antibodies are imaged that are used to bind to their antigens." [ECO:SN, url:http\://journals.plos.org/plosone/article?id=10.1371/journal.pone.0057135] +synonym: "wide-field epifluorescence microscopy evidence" BROAD [] +is_a: ECO:0005599 ! immunofluorescence wide-field microscopy evidence +is_a: ECO:0005804 ! immunofluorescence evidence used in manual assertion +is_a: ECO:0006062 ! wide-field fluorescence microscopy evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-05-15T08:57:07Z + +[Term] +id: ECO:0006062 +name: wide-field fluorescence microscopy evidence used in manual assertion +def: "A type of wide-field microscopy evidence that is used in a manual assertion where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter." [ECO:SN, url:http\://www.bristol.ac.uk/synaptic/research/techniques/widefield.html] +is_a: ECO:0005590 ! wide-field microscopy evidence used in manual assertion +is_a: ECO:0005598 ! wide-field fluorescence microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-05-15T09:01:19Z + +[Term] +id: ECO:0006063 +name: over expression analysis evidence used in manual assertion +def: "A type of experimental phenotypic evidence that is used in a manual assertion where a gene and/or gene product is investigated in a transgenic organism that has been engineered to overexpress that gene product." [PMID:22419077] +synonym: "analysis of overexpression/ectopic expression phenotype" RELATED [] +is_a: ECO:0000120 ! over expression analysis evidence +is_a: ECO:0006049 ! genetic transformation evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2017-05-15T09:03:49Z + +[Term] +id: ECO:0006064 +name: cell-free assay evidence used in manual assertion +def: "A type of cell-free assay evidence that is used in a manual assertion." [PMID:18453125] +synonym: "in vitro assay evidence" BROAD [] +is_a: ECO:0000183 ! cell-free assay evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-05-15T09:08:17Z + +[Term] +id: ECO:0006065 +name: in vitro cell based assay evidence used in manual assertion +comment: Use children of ECO:0001565 (cell-based assay evidence) in place of this term. +is_obsolete: true +created_by: rctauber +creation_date: 2017-05-15T09:11:13Z + +[Term] +id: ECO:0006066 +name: fluorescence recovery after photobleaching evidence +def: "A type of fluorescence evidence where quantitative information on the diffusion properties of a sample is produced from the measurement of diffusion of fluorescent probes over time into an area that has been photobleached by a high-intensity laser pulse." [PMID:26314367] +comment: FRAP is a fluorescence microscopy-based technique. {xref="PMID:26314367"} +synonym: "FRAP evidence" EXACT [] +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000234 "dosumis" xsd:string +created_by: rctauber +creation_date: 2017-05-16T10:55:40Z + +[Term] +id: ECO:0006067 +name: fluorescence recovery after photobleaching evidence used in manual assertion +def: "A type of fluorescence evidence that is used in a manual assertion where quantitative information on the diffusion properties of a sample is produced from the measurement of diffusion of fluorescent probes over time into an area that has been photobleached by a high-intensity laser pulse." [] +synonym: "FRAP evidence" BROAD [] +is_a: ECO:0001249 ! fluorescence evidence used in manual assertion +is_a: ECO:0006066 ! fluorescence recovery after photobleaching evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-05-16T10:55:40Z + +[Term] +id: ECO:0006068 +name: RNA-sequencing evidence used in manual assertion +def: "A type of high throughput nucleotide sequencing assay evidence used in a manual assertion based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC] +synonym: "RNA sequencing|differential gene expression evidence from RNA-seq experiment" RELATED [] +synonym: "RNA-seq evidence used in manual assertion" EXACT [] +synonym: "RNAseq evidence used in manual assertion" EXACT [] +synonym: "whole transcriptome shotgun sequencing" BROAD [] +synonym: "WTSS evidence" BROAD [] +is_a: ECO:0000291 ! transcript expression evidence used in manual assertion +is_a: ECO:0000295 ! RNA-sequencing evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2017-06-28T10:37:02Z + +[Term] +id: ECO:0006069 +name: RNA-sequencing evidence used in automatic assertion +def: "A type of high throughput nucleotide sequencing assay evidence used in an automatic assertion based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC] +synonym: "RNA sequencing|differential gene expression evidence from RNA-seq experiment" RELATED [] +synonym: "RNA-seq evidence used in automatic assertion" EXACT [] +synonym: "RNAseq evidence used in automatic assertion" EXACT [] +synonym: "whole transcriptome shotgun sequencing" BROAD [] +synonym: "WTSS evidence" BROAD [] +is_a: ECO:0000295 ! RNA-sequencing evidence +is_a: ECO:0007303 ! transcript expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2017-06-28T10:37:02Z + +[Term] +id: ECO:0006070 +name: immuno-labelling electron microscopy evidence +def: "A type of electron microscopy evidence resulting from the use of antibodies to identify the localization of antigens in cells and tissues." [PMID:25151300] +synonym: "immunolabelling electron microscopy evidence" EXACT [] +is_a: ECO:0005033 ! electron microscopy evidence +property_value: IAO:0000234 "dosumis" xsd:string +created_by: rctauber +creation_date: 2017-07-05T09:59:10Z + +[Term] +id: ECO:0006071 +name: immuno-labelling electron microscopy evidence used in manual assertion +def: "A type of electron microscopy evidence used in a manual assertion resulting from the use of antibodies to identify the localization of antigens in cells and tissues." [PMID:25151300] +synonym: "immunolabelling electron microscopy evidence" BROAD [] +is_a: ECO:0006003 ! electron microscopy evidence used in manual assertion +is_a: ECO:0006070 ! immuno-labelling electron microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000119 PMID:25151300 xsd:string +property_value: IAO:0000234 "dosumis" xsd:string +created_by: rctauber +creation_date: 2017-07-05T09:59:10Z + +[Term] +id: ECO:0006072 +name: immunofluorescence super resolution microscopy evidence +def: "A type of super-resolution microscopy evidence resulting from the use of fluorescently-tagged antibodies to identify the localization of antigens in cells and tissue." [PMID:19245833] +is_a: ECO:0005578 ! super-resolution microscopy evidence +property_value: IAO:0000234 "dosumis" xsd:string +created_by: rctauber +creation_date: 2017-07-05T09:59:10Z + +[Term] +id: ECO:0006073 +name: immunofluorescence super resolution microscopy evidence used in manual assertion +def: "A type of super-resolution microscopy evidence used in a manual assertion resulting from the use of fluorescently-tagged antibodies to identify the localization of antigens in cells and tissue." [PMID:19245833] +is_a: ECO:0006004 ! super-resolution microscopy evidence used in manual assertion +is_a: ECO:0006072 ! immunofluorescence super resolution microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "dosumis" xsd:string +created_by: rctauber +creation_date: 2017-07-05T09:59:10Z + +[Term] +id: ECO:0006074 +name: co-purification evidence used in manual assertion +def: "A type of physical interaction evidence that is used in manual assertion where a cellular component subunit is isolated as part of purification of its larger complex." [TAIR:TED] +synonym: "co-purification" BROAD [] +is_a: ECO:0000022 ! co-purification evidence +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:19Z + +[Term] +id: ECO:0006075 +name: affinity evidence used in manual assertion +def: "A type of physical interaction evidence that is used in manual assertion that depends on the strength of the interaction between two entities." [ECO:MCC] +synonym: "ligand binding evidence" RELATED [] +is_a: ECO:0000023 ! affinity evidence +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:20Z + +[Term] +id: ECO:0006076 +name: protein binding evidence used in manual assertion +def: "A type of affinity evidence that is used in manual assertion resulting from the binding of a molecule to a protein or protein complex." [GO:0005515] +is_a: ECO:0000024 ! protein binding evidence +is_a: ECO:0006075 ! affinity evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:21Z + +[Term] +id: ECO:0006077 +name: bait-prey hybrid interaction evidence used in manual assertion +def: "A type of bait-prey hybrid interaction evidence that is used in manual assertion where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC] +synonym: "bait-prey protein pull-down evidence" EXACT [] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence +is_a: ECO:0006076 ! protein binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:22Z + +[Term] +id: ECO:0006078 +name: immunological assay evidence used in manual assertion +def: "A type of affinity evidence that is used in manual assertion resulting from quantitation of the analyte which depends on the reaction of an antigen (analyte) and an antibody." [ERO:0001362] +is_a: ECO:0000040 ! immunological assay evidence +is_a: ECO:0006075 ! affinity evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:23Z + +[Term] +id: ECO:0006079 +name: yeast one-hybrid evidence used in manual assertion +def: "A type of hybrid interaction evidence that is used in manual assertion that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors." [ECO:MCC] +synonym: "yeast one-hybrid assay" BROAD [] +is_a: ECO:0000066 ! yeast one-hybrid evidence +is_a: ECO:0006077 ! bait-prey hybrid interaction evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:24Z + +[Term] +id: ECO:0006080 +name: split-ubiquitin functional complementation evidence used in manual assertion +def: "A type of split-ubiquitin functional complementation evidence that is used in a manual assertion that is based on detection of protein-protein interaction between a bait and prey protein by in vivo reconstitution of split-ubiquitin (when bait and prey interact) and release of a reporter protein." [PMID:15064465] +synonym: "split-ubiquitin assay" BROAD [] +is_a: ECO:0000074 ! split-ubiquitin functional complementation evidence +is_a: ECO:0006293 ! protein fragment functional complementation evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:25Z + +[Term] +id: ECO:0006081 +name: far-Western blotting evidence used in manual assertion +def: "A type of physical interaction evidence that is used in a manual assertion that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes." [PMID:18079728] +synonym: "far-Western analysis" BROAD [] +is_a: ECO:0000076 ! far-Western blotting evidence +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:26Z + +[Term] +id: ECO:0006082 +name: affinity chromatography evidence used in manual assertion +def: "A type of affinity evidence that is used in a manual assertion that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bond compound." [ECO:MCC] +synonym: "affinity chromatography" BROAD [] +is_a: ECO:0000079 ! affinity chromatography evidence +is_a: ECO:0006075 ! affinity evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:27Z + +[Term] +id: ECO:0006083 +name: nucleic acid binding evidence used in manual assertion +def: "A type of affinity evidence that is used in a manual assertion resulting from the binding of a molecule to a nucleic acid." [GO:0003676] +is_a: ECO:0000136 ! nucleic acid binding evidence +is_a: ECO:0006075 ! affinity evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:28Z + +[Term] +id: ECO:0006084 +name: ribohomopolymer binding assay evidence used in manual assertion +def: "A type of nucleic acid binding evidence that is used in a manual assertion resulting from an enzyme displaying binding activity to specific ribohomopolymer." [PMC:102612] +synonym: "ribohomopolymer binding assay" BROAD [] +is_a: ECO:0000138 ! ribohomopolymer binding assay evidence +is_a: ECO:0006083 ! nucleic acid binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:29Z + +[Term] +id: ECO:0006085 +name: protein:ion binding evidence used in manual assertion +def: "A type of protein binding evidence that is used in a manual assertion resulting from a metal ion binding to a protein at a specific binding site." [PMID:2377604] +is_a: ECO:0000142 ! protein:ion binding evidence +is_a: ECO:0006076 ! protein binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:30Z + +[Term] +id: ECO:0006086 +name: Southwestern blot evidence used in manual assertion +def: "A type of nucleic acid binding evidence that is used in a manual assertion in which DNA-protein binding is detected using labeled DNA as probes, hybridized to electrophoretically separated proteins." [ECO:RCT] +synonym: "Southwestern analysis" BROAD [] +is_a: ECO:0000144 ! Southwestern blot evidence +is_a: ECO:0006083 ! nucleic acid binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:31Z + +[Term] +id: ECO:0006087 +name: Northwestern blot evidence used in manual assertion +def: "A type of nucleic acid binding evidence that is used in a manual assertion in which RNA-protein binding is detected using labeled RNA as probes, hybridized to electrophoretically separated proteins." [ECO:RCT] +synonym: "Northwestern analysis" BROAD [] +is_a: ECO:0000146 ! Northwestern blot evidence +is_a: ECO:0006083 ! nucleic acid binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:32Z + +[Term] +id: ECO:0006088 +name: systematic evolution of ligands by exponential amplification evidence used in manual assertion +def: "A type of evidence that is used in a manual assertion arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand." [ECO:MCC] +synonym: "in vitro evolution evidence" RELATED [] +synonym: "in vitro selection evidence" BROAD [] +synonym: "SELEX evidence" BROAD [] +is_a: ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:33Z + +[Term] +id: ECO:0006089 +name: bacterial one-hybrid evidence used in manual assertion +def: "A type of hybrid interaction evidence that is used in a manual assertion that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey)." [ECO:MCC] +synonym: "B1H evidence" BROAD [] +is_a: ECO:0000294 ! bacterial one-hybrid evidence +is_a: ECO:0006077 ! bait-prey hybrid interaction evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:34Z + +[Term] +id: ECO:0006090 +name: protein-oligonucleotide microarray binding evidence used in manual assertion +def: "A type of protein-oligonucleotide microarray binding evidence that is used in a manual assertion that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites." [PMID:22146299] +synonym: "PBM evidence" BROAD [] +is_a: ECO:0001601 ! protein-oligonucleotide microarray binding evidence +is_a: ECO:0006076 ! protein binding evidence used in manual assertion +is_a: ECO:0006083 ! nucleic acid binding evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:35Z + +[Term] +id: ECO:0006091 +name: functional complementation evidence used in manual assertion +def: "A type of genetic interaction evidence that is used in a manual assertion where a wild-type copy of the gene in question is inserted into a mutant cell to see if it restores the wild-type phenotype in the mutant background." [PMID:27403640] +synonym: "functional complementation" BROAD [] +is_a: ECO:0000012 ! functional complementation evidence +is_a: ECO:0000316 ! genetic interaction evidence used in manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:36Z + +[Term] +id: ECO:0006092 +name: transgenic rescue experiment evidence used in manual assertion +def: "A type of functional complementation evidence that is used in a manual assertion that is used in manual assertion resulting from the introduction of a transgene to prevent, or \"rescue\" an organism from a condition." [url:http\://www.nature.com/gt/journal/v11/n15/full/3302282a.html] +is_a: ECO:0000013 ! transgenic rescue experiment evidence +is_a: ECO:0006091 ! functional complementation evidence used in manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:37Z + +[Term] +id: ECO:0006093 +name: transient rescue experiment evidence used in manual assertion +def: "A type of transient rescue experiment evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000038 ! transient rescue experiment evidence +is_a: ECO:0006091 ! functional complementation evidence used in manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:38Z + +[Term] +id: ECO:0006094 +name: suppressor/enhancer interaction phenotypic evidence used in manual assertion +def: "A type of suppressor/enhancer interaction phenotypic evidence that is used in a manual assertion." [url:http\://www.wormbook.org/chapters/www\:geneticsuppression/geneticsuppression.html] +synonym: "'traditional' genetic interactions (e.g. suppressors, synthetic lethals)" BROAD [] +synonym: "suppressor/enhancer interaction evidence used in manual assertion" EXACT [] +is_a: ECO:0000052 ! suppressor/enhancer interaction phenotypic evidence +is_a: ECO:0000316 ! genetic interaction evidence used in manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:39Z + +[Term] +id: ECO:0006095 +name: double mutant phenotypic evidence used in manual assertion +def: "A type of double mutant phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "double mutant analysis" BROAD [] +synonym: "double mutant phenotype evidence used in manual assertion" EXACT [] +is_a: ECO:0000054 ! double mutant phenotypic evidence +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:40Z + +[Term] +id: ECO:0006096 +name: epistatic interaction phenotypic evidence used in manual assertion +def: "A type of epistatic interaction phenotypic evidence that is used in a manual assertion." [PMID:18852697] +synonym: "epistatic interaction evidence used in manual assertion" EXACT [] +synonym: "epistatic interactions" BROAD [] +is_a: ECO:0000056 ! epistatic interaction phenotypic evidence +is_a: ECO:0000316 ! genetic interaction evidence used in manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:41Z + +[Term] +id: ECO:0006097 +name: functional complementation in heterologous system evidence used in manual assertion +def: "A type of functional complementation evidence that is used in a manual assertion that is based on the insertion of a wild-type copy of a gene into a heterologous organism, with the mutation occurring in a homologous gene." [TAIR:TED] +synonym: "functional complementation in heterologous system" BROAD [] +is_a: ECO:0000064 ! functional complementation in heterologous system evidence +is_a: ECO:0006091 ! functional complementation evidence used in manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:42Z + +[Term] +id: ECO:0006098 +name: temperature-sensitive mutant phenotypic evidence +def: "A type of mutant phenotype evidence resulting from altered gene function at higher temperatures." [GOC:PG, PMID:19596904] +synonym: "temperature-sensitive mutant phenotype evidence" EXACT [] +synonym: "Ts mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000234 "pgaudet" xsd:string +created_by: rctauber +creation_date: 2017-09-18T07:58:28Z + +[Term] +id: ECO:0006099 +name: temperature-sensitive mutant phenotypic evidence used in manual assertion +def: "A type of temperature-sensitive mutant phenotypic evidence that is used in a manual assertion." [GOC:PG, PMID:19596904] +synonym: "temperature-sensitive mutant phenotype evidence used in manual assertion" EXACT [] +synonym: "Ts mutation evidence" BROAD [] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0006098 ! temperature-sensitive mutant phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 "pgaudet" xsd:string +created_by: rctauber +creation_date: 2017-09-18T07:58:28Z + +[Term] +id: ECO:0006100 +name: recessive mutant phenotype evidence +def: "A type of mutant phenotype evidence based on the analysis of a mutation which, in diploid organisms, must be present in both alleles for a phenotype to manifest itself." [GOC:PG, NBK:21578] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000234 "pgaudet" xsd:string +created_by: rctauber +creation_date: 2017-09-18T07:58:28Z + +[Term] +id: ECO:0006101 +name: recessive mutant phenotype evidence used in manual assertion +def: "A type of mutant phenotype evidence that is used in a manual assertion based on the analysis of a mutation which, in diploid organisms, must be present in both alleles for a phenotype to manifest itself." [GOC:PG, NBK:21578] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0006100 ! recessive mutant phenotype evidence +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 "pgaudet" xsd:string +created_by: rctauber +creation_date: 2017-09-18T07:58:28Z + +[Term] +id: ECO:0006135 +name: quantum mechanics/molecular mechanics simulation evidence +def: "A type of computational evidence where the active site of the molecular system is described with highly accurate quantum theory, while the contribution of the rest of the system is described with molecular mechanical force field." [ECO:SN, PMID:26930454] +synonym: "QM/MM evidence" EXACT [] +is_a: ECO:0007672 ! computational evidence +created_by: snadendla + +[Term] +id: ECO:0006136 +name: quantum mechanics/molecular mechanics simulation evidence used in automatic assertion +def: "A type of quantum mechanics/molecular mechanics simulation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006135 ! quantum mechanics/molecular mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006137 +name: quantum mechanics/molecular mechanics simulation evidence used in manual assertion +def: "A type of quantum mechanics/molecular mechanics simulation evidence that is used in manual assertion." [ECO:SN] +is_a: ECO:0006135 ! quantum mechanics/molecular mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006138 +name: molecular mechanics simulation evidence +def: "A type of computational evidence where the energy of a molecular system is predicted as a function of its conformation." [ECO:SN, url:http\://vergil.chemistry.gatech.edu/courses/chem6485/pdf/molmech.pdf] +synonym: "MM evidence" EXACT [] +is_a: ECO:0007672 ! computational evidence +created_by: snadendla + +[Term] +id: ECO:0006139 +name: molecular mechanics simulation evidence used in automatic assertion +def: "A type of molecular mechanics simulation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006138 ! molecular mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006140 +name: molecular mechanics simulation evidence used in manual assertion +def: "A type of molecular mechanics simulation evidence that is used in manual assertion." [ECO:SN] +is_a: ECO:0006138 ! molecular mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006141 +name: quantum mechanics simulation evidence +def: "A type of computational evidence where the behavior of matter and light on the atomic and subatomic scale is described." [ECO:SN, url:https\://www.britannica.com/science/quantum-mechanics-physics] +synonym: "QM evidence" EXACT [] +synonym: "quantum physics evidence" EXACT [] +synonym: "quantum theory evidence" EXACT [] +is_a: ECO:0007672 ! computational evidence +created_by: snadendla + +[Term] +id: ECO:0006142 +name: quantum mechanics simulation evidence used in automatic assertion +def: "A type of quantum mechanics simulation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006141 ! quantum mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006143 +name: quantum mechanics simulation evidence used in manual assertion +def: "A type of quantum mechanics simulation evidence that is used in manual assertion." [ECO:SN] +is_a: ECO:0006141 ! quantum mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006144 +name: density functional theory simulation evidence +def: "A type of quantum mechanics simulation evidence that results from the calculation of ground-state electronic structure of atoms, molecules and solid state materials." [ECO:SN, url:https\://www.sciencedirect.com/topics/physics-and-astronomy/density-functional-theory] +synonym: "DFT evidence" EXACT [] +is_a: ECO:0006141 ! quantum mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006145 +name: density functional theory simulation evidence used in manual assertion +def: "A type of density functional theory simulation evidence that is used in manual assertion." [ECO:SN] +is_a: ECO:0006144 ! density functional theory simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006146 +name: density functional theory simulation evidence used in automatic assertion +def: "A type of density functional theory simulation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006144 ! density functional theory simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006151 +name: documented statement evidence +def: "A type of evidence in which information is recorded in some documentation system, for example, but not limited to a publication, survey, or medical record." [ECO:SN] +is_a: ECO:0000000 ! evidence +created_by: snadendla + +[Term] +id: ECO:0006152 +name: medical practitioner statement evidence +def: "A type of documented statement evidence in which information is stated by a health care professional, for example, but not limited to a doctor, nurse, or psychologist." [ECO:SN] +is_a: ECO:0006151 ! documented statement evidence +created_by: snadendla + +[Term] +id: ECO:0006153 +name: self-reported individual's statement evidence +def: "A type of documented statement evidence in which information is provided by an individual through means including, but not limited to, paper form, electronic form, or verbal communication, and is captured in a documented record." [ECO:SN] +is_a: ECO:0006151 ! documented statement evidence +created_by: snadendla + +[Term] +id: ECO:0006154 +name: self-reported patient statement evidence +def: "A type of self-reported individual's statement evidence in which information is provided by a patient in a clinical setting." [ECO:SN] +is_a: ECO:0006153 ! self-reported individual's statement evidence +property_value: IAO:0000234 "Pablo Botas for Foundation 29, Dx29 project" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006155 +name: documented statement evidence used in manual assertion +def: "A type of documented statement evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0006151 ! documented statement evidence +created_by: snadendla + +[Term] +id: ECO:0006156 +name: documented statement evidence used in automatic assertion +def: "A type of documented statement evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0000501 ! evidence used in automatic assertion +is_a: ECO:0006151 ! documented statement evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006157 +name: self-reported individual's statement evidence used in manual assertion +def: "A type of self-reported individual's statement evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006153 ! self-reported individual's statement evidence +is_a: ECO:0006155 ! documented statement evidence used in manual assertion +created_by: snadendla + +[Term] +id: ECO:0006158 +name: self-reported individual's statement evidence used in automatic assertion +def: "A type of self-reported individual's statement evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006153 ! self-reported individual's statement evidence +is_a: ECO:0006156 ! documented statement evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006159 +name: self-reported patient statement evidence used in manual assertion +def: "A type of self-reported patient statement evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006154 ! self-reported patient statement evidence +is_a: ECO:0006157 ! self-reported individual's statement evidence used in manual assertion +created_by: snadendla + +[Term] +id: ECO:0006160 +name: self-reported patient statement evidence used in automatic assertion +def: "A type of self-reported patient statement evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006154 ! self-reported patient statement evidence +is_a: ECO:0006158 ! self-reported individual's statement evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006161 +name: medical practitioner statement evidence used in manual assertion +def: "A type of medical practitioner statement evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006152 ! medical practitioner statement evidence +is_a: ECO:0006155 ! documented statement evidence used in manual assertion +created_by: snadendla + +[Term] +id: ECO:0006162 +name: medical practitioner statement evidence used in automatic assertion +def: "A type of medical practitioner statement evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006152 ! medical practitioner statement evidence +is_a: ECO:0006156 ! documented statement evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006163 +name: nuclear magnetic resonance spectroscopy evidence +def: "A type of nuclear magnetic resonance evidence used for quantification of metabolites or for the determination of chemical structure or composition." [ECO:SN, PMID: 16428685, PMID: 23036848] +synonym: "NMR Spectroscopy" EXACT [] +is_a: ECO:0001105 ! nuclear magnetic resonance evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: snadendla + +[Term] +id: ECO:0006164 +name: nuclear magnetic resonance spectroscopy evidence used in automatic assertion +def: "A type of nuclear magnetic resonance spectroscopy evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence +is_a: ECO:0007441 ! nuclear magnetic resonance evidence used in automatic assertion +property_value: IAO:0000234 SIB:PG xsd:string +created_by: snadendla + +[Term] +id: ECO:0006165 +name: nuclear magnetic resonance spectroscopy evidence used in manual assertion +def: "A type of nuclear magnetic resonance spectroscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "NMR spectroscopy evidence" BROAD [] +is_a: ECO:0001238 ! nuclear magnetic resonance evidence used in manual assertion +is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: snadendla + +[Term] +id: ECO:0006166 +name: nuclear magnetic resonance imaging evidence +def: "A type of nuclear magnetic resonance evidence used to image anatomy and physiological processes." [url:https\://en.wikipedia.org/wiki/Magnetic_resonance_imaging] +is_a: ECO:0001105 ! nuclear magnetic resonance evidence +property_value: IAO:0000234 "bjonnh" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006167 +name: nuclear magnetic resonance imaging evidence used in automatic assertion +def: "A type of nuclear magnetic resonance imaging evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006166 ! nuclear magnetic resonance imaging evidence +is_a: ECO:0007441 ! nuclear magnetic resonance evidence used in automatic assertion +property_value: IAO:0000234 "bjonnh" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006168 +name: nuclear magnetic resonance imaging evidence used in manual assertion +def: "A type of nuclear magnetic resonance imaging evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001238 ! nuclear magnetic resonance evidence used in manual assertion +is_a: ECO:0006166 ! nuclear magnetic resonance imaging evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "bjonnh" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006169 +name: quantitative western immunoblotting evidence +def: "A type of qualitative western immunoblotting evidence where detection of the signal is carried out through digital imaging and the signal is normalized with methods such as standard curves built from reference housekeeping proteins or total transferred protein measurements." [ECO:SN, PMID:25852189] +is_a: ECO:0000112 ! qualitative western immunoblotting evidence +property_value: IAO:0000234 GO:Val xsd:string +created_by: snadendla + +[Term] +id: ECO:0006170 +name: quantitative western immunoblotting evidence used in manual evidence +def: "A type of quantitative western immunoblotting evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000279 ! qualitative western immunoblotting evidence used in manual assertion +is_a: ECO:0006169 ! quantitative western immunoblotting evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006171 +name: quantitative western immunoblotting evidence used in automatic assertion +def: "A type of quantitatative western immunoblotting evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006169 ! quantitative western immunoblotting evidence +is_a: ECO:0007306 ! qualitative western immunoblotting evidence used in automatic assertion +created_by: snadendla + +[Term] +id: ECO:0006172 +name: mixed support of intron positions by RNA-sequencing alignment evidence +def: "A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignments from a mixture of samples support all of the intron positions (exon pairs) predicted for a transcript." [ECO:SN] +comment: Mixed support entails that all exon pairs represented in the transcript are supported, but require a combination of evidence from multiple samples. For example, for a transcript containing five exons, if liver RNA-seq reads support the first two introns and brain RNA-seq supports the last three introns, then neither sample meets the criteria for full support (ECO:0000343) but the combination of samples qualifies as Mixed support. Mixed support may result from differential expression, or low expression levels that benefit from combining data. +synonym: "mixed support of intron positions by RNA-seq alignment evidence" EXACT [] +is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence +property_value: IAO:0000234 "NCBI-RefSeq" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006173 +name: mixed support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +def: "A type of mixed support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." [ECO:SN] +synonym: "mixed support of intron positions by RNA-seq alignment evidence used in automatic assertion" EXACT [] +is_a: ECO:0000347 ! support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +is_a: ECO:0006172 ! mixed support of intron positions by RNA-sequencing alignment evidence +property_value: IAO:0000234 "NCBI-RefSeq" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006174 +name: mixed support of intron positions by RNA-sequencing alignment evidence used in manual assertion +def: "A type of mixed support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion" [ECO:SN] +synonym: "mixed support of intron positions by RNA-seq alignment evidence used in manual assertion" EXACT [] +is_a: ECO:0000346 ! support of intron positions by RNA-sequencing alignment evidence used in manual assertion +is_a: ECO:0006172 ! mixed support of intron positions by RNA-sequencing alignment evidence +property_value: ECO:9000002 "IEP" xsd:string +property_value: IAO:0000234 "NCBI-RefSeq" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006175 +name: nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence +def: "A type of nuclear magnetic resonance spectroscopy evidence resulting from measuring the change in a certain type of NMR spectrum as a result of the spontenaous exchange of hydrogen and deuterium nuclei between the sample and the solvent." [PMID:20960033, PMID:28538145, url:https\://en.wikipedia.org/wiki/Hydrogen%E2%80%93deuterium_exchange#NMR_spectroscopy] +synonym: "NMR HDX" EXACT [] +is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006176 +name: proton-based nuclear magnetic resonance evidence +def: "A type of nuclear magnetic resonance spectroscopy evidence in which the electromagnetic singal being measured comes from the perturbation of hydrogen nuclei resulting in a one dimensional spectrum." [url:https\://en.wikipedia.org/wiki/Proton_nuclear_magnetic_resonance] +synonym: "1D NMR" BROAD [] +synonym: "proton NMR" EXACT [] +is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006177 +name: circular dichroism evidence +def: "A type of spectrometry evidence derived from irradiating a sample with light (either visible, infrared or ultraviolet wavelengths) and observing the difference of absorption of left-handed and right-handed light by the sample by comparing the extent of circular polarization in the incident and the emitted light." [PMID:17464384, url:https\://en.wikipedia.org/wiki/Circular_dichroism] +synonym: "CD" EXACT [] +is_a: ECO:0005031 ! structure determination evidence +is_a: ECO:0005504 ! spectrometry evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006178 +name: synchrotron radiation circular dichroism evidence +def: "A type of circular dichroism evidence where the source of radiation is radially accelerated particles, most often moving on a circular path in a synchrotron, typically providing lower wavelengths and therefore higher signal-to-noise ratio compared to regular circular dichroism measurements." [PMID:20658968] +synonym: "SRCD" EXACT [] +is_a: ECO:0006177 ! circular dichroism evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006179 +name: far-UV circular dichroism evidence +def: "A type of circular dichroism evidence whose spectrum is in the range of 190-230 nm, providing information mainly about amide bonds and through which the relative proportion of secondary structure elements of a protein can be estimated." [PMID:17464384] +synonym: "far-UV CD" EXACT [] +is_a: ECO:0006177 ! circular dichroism evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006180 +name: near-UV circular dichroism evidence +def: "A type of circular dichroism evidence whose spectrum is in the range of 250-350 nm , providing information mainly about aromatic residues, i.e. phenylalanine, tyrosine and tryptophan, through which it provides information on the tertiary structure of a protein." [PMID:17464384] +synonym: "near-UV CD" EXACT [] +is_a: ECO:0006177 ! circular dichroism evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006181 +name: cryogenic electron microscopy evidence +def: "A type of electron microscopy evidence where the sample being studied has been cooled to cryogenic temperatures (typically below -153 degrees Celsius) prior to the experiment." [PMID:31078399, url:https\://en.wikipedia.org/wiki/Cryogenic_electron_microscopy] +synonym: "cryo-EM" EXACT [] +is_a: ECO:0005033 ! electron microscopy evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006182 +name: small-angle X-ray scattering evidence +def: "A type of light scattering evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight and structural transitions by irradiating the sample with an X-ray beam and the scattered intensity is probed at small angles (typically in the range of 0.1-10 degrees) by a detector." [PMID:26320411, url:https\://en.wikipedia.org/wiki/Small-angle_X-ray_scattering] +synonym: "SAXS" EXACT [] +is_a: ECO:0007061 ! light scattering evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006183 +name: particle scattering evidence +def: "A type of structure determination evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight, diffusion and interaction strength by irradiating the sample with a particle beam and the scattered intensity is probed at a certain angle by a detector." [DisProt:BalintMeszaros] +is_a: ECO:0005031 ! structure determination evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006184 +name: small-angle neutron scattering evidence +def: "A type of particle scattering evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight and structural transitions by irradiating the sample with a beam of thermal neutrons and the scattered intensity is probed at small angles (typically in the range of 0.1-10 degrees) by a detector." [PMID:17714935, url:https\://en.wikipedia.org/wiki/Small-angle_neutron_scattering] +synonym: "SANS" EXACT [] +is_a: ECO:0006183 ! particle scattering evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006185 +name: author inference +def: "A type of inferential evidence where an assertion is derived by the authors of a scientific publication from another assertion and/or primary data via logical inference or other rational means." [DisProt:BalintMeszaros] +is_a: ECO:0000204 ! author statement +is_a: ECO:0000361 ! inferential evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006186 +name: combinatorial experimental and author inference evidence contained in single publication +def: "A type of combinatorial experimental and author inference evidence where the experimental results and author inference are contained in a single publication. " [ECO:MG] +is_a: ECO:0007011 ! combinatorial experimental and author inference evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006187 +name: X-ray crystallography-based structural model with missing residue coordinates +def: "A type of X-ray cystallography evidence in which a structural model is built lacking coordinates for some residues indicating high flexibility or structural disorder in that area of the molecule." [DisProt:BalintMeszaros] +is_a: ECO:0001823 ! x-ray crystallography evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006188 +name: X-ray crystallography-based structural model with high relative B-factor values +def: "A type of X-ray cystallography evidence in which a structural model is built that contains high relative B-factor values indicating flexibility in that area of the molecule." [DisProt:BalintMeszaros] +is_a: ECO:0001823 ! x-ray crystallography evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006189 +name: cryogenic electron microscopy-based structural model with missing residue coordinates +def: "A type of cryogenic electron microscopy evidence in which a structural model is built lacking coordinates for some residues indicating high flexibility or structural disorder in that area of the molecule." [DisProt:BalintMeszaros] +is_a: ECO:0006181 ! cryogenic electron microscopy evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006190 +name: cryogenic electron microscopy-based structural model with high relative B-factor values +def: "A type of cryogenic electron microscopy evidence in which a structural model is built that contains high relative B-factor values indicating flexibility in that area of the molecule." [DisProt:BalintMeszaros] +is_a: ECO:0006181 ! cryogenic electron microscopy evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006191 +name: Fourier-transform infrared spectroscopy evidence +def: "A type of spectrophotometry evidence resulting from the evaluation of a molecule in a fluid or solid state by its ability to alter the transmission of infrared light, where the raw data collected is transformed into the specturm by Fourier transform." [url:https\://en.wikipedia.org/wiki/Fourier-transform_infrared_spectroscopy] +synonym: "FT-IR" EXACT [] +synonym: "FTIR" EXACT [] +is_a: ECO:0001126 ! spectrophotometry evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006192 +name: heat capacity-based evidence +def: "A type of direct assay evidence derived from measuring the amount of heat absorbed or released by a sample to monitor chemical reactions, phase transitions or other processes affecting the physical state or chemical composition." [url:https\://en.wikipedia.org/wiki/Calorimetry] +synonym: "calorimetry evidence" BROAD [] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006193 +name: differential scanning calorimetry evidence +def: "A type of heat capacity-based evidence where the heat required to increase the temperature of the sample is continuously monitored and compared to the heat required to increase the temperature of a reference (having a largely identical composition compared to the sample, such as the empty buffer compared to a protein solution) over a range of temperatures." [url:https\://en.wikipedia.org/wiki/Differential_scanning_calorimetry] +synonym: "DSC" EXACT [] +is_a: ECO:0006192 ! heat capacity-based evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006194 +name: selective antibody-based structural conformation evidence +def: "A type of immunodetection assay evidence that involves the use of antibodies that are selective to conformations of the epitope to which they bind in order to provide information on conformations present in a target molecule." [DisProt:BalintMeszaros] +is_a: ECO:0005031 ! structure determination evidence +is_a: ECO:0005593 ! immunodetection assay evidence +property_value: IAO:0000112 "Here, we describe the generation, characterisation, and utility of a monoclonal antibody that selectively binds with high affinity to the asymmetric TNF trimer–small molecule complex. The antibody helps to define the molecular dynamics of the apo TNF trimer, reveals the mode of action and specificity of the small molecule inhibitors, acts as a chaperone in solving the human TNF–TNFR1 complex crystal structure, and facilitates the measurement of small molecule target occupancy in complex biological samples." xsd:string {xref="PMID:33495445"} +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006195 +name: protein hydrogen-deuterium exchange mass spectrometry evidence +def: "A type of mass spectrometry evidence derived from measuring changes in mass associated with the isotopic exchange between amide hydrogens of the protein backbone and its surrounding solvent, providing information on the structural state, the dynamics and the intrinsic chemical properties of the underlying amino acid sequence." [PMID:31249422, url:https\://en.wikipedia.org/wiki/Hydrogen%E2%80%93deuterium_exchange#Mass_spectrometry] +synonym: "HDX-MS" EXACT [] +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006196 +name: nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence used in manual assertion +def: "A type of nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence that is used in a manual assertion." [ECO:SN] +synonym: "NMR HDX" EXACT [] +is_a: ECO:0006165 ! nuclear magnetic resonance spectroscopy evidence used in manual assertion +is_a: ECO:0006175 ! nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006197 +name: nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence used in automatic assertion +def: "A type of nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence that is used in an automatic assertion." [ECO:SN] +synonym: "NMR HDX" EXACT [] +is_a: ECO:0006164 ! nuclear magnetic resonance spectroscopy evidence used in automatic assertion +is_a: ECO:0006175 ! nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006198 +name: proton-based nuclear magnetic resonance evidence used in manual assertion +def: "A type of proton-based nuclear magnetic resonance evidence that is used in a manual assertion." [ECO:SN] +synonym: "1D NMR" BROAD [] +synonym: "proton NMR" EXACT [] +is_a: ECO:0006165 ! nuclear magnetic resonance spectroscopy evidence used in manual assertion +is_a: ECO:0006176 ! proton-based nuclear magnetic resonance evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006199 +name: proton-based nuclear magnetic resonance evidence used in automatic assertion +def: "A type of proton-based nuclear magnetic resonance evidence that is used in an automatic assertion." [ECO:SN] +synonym: "1D NMR" BROAD [] +synonym: "proton NMR" EXACT [] +is_a: ECO:0006164 ! nuclear magnetic resonance spectroscopy evidence used in automatic assertion +is_a: ECO:0006176 ! proton-based nuclear magnetic resonance evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006200 +name: circular dichroism evidence used in manual assertion +def: "A type of circular dichroism evidence that is used in a manual assertion." [ECO:SN] +synonym: "CD" EXACT [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0006177 ! circular dichroism evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006201 +name: circular dichroism evidence used in automatic assertion +def: "A type of circular dichroism evidence that is used in an automatic assertion." [ECO:SN] +synonym: "CD" EXACT [] +is_a: ECO:0006177 ! circular dichroism evidence +is_a: ECO:0007815 ! structure determination evidence used in automatic assertion +is_a: ECO:0007821 ! spectrometry evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006202 +name: synchrotron radiation circular dichroism evidence used in manual assertion +def: "A type of synchrotron radiation circular dichroism evidence that is used in a manual assertion." [ECO:SN] +synonym: "SRCD" EXACT [] +is_a: ECO:0006178 ! synchrotron radiation circular dichroism evidence +is_a: ECO:0006200 ! circular dichroism evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006203 +name: synchrotron radiation circular dichroism evidence used in automatic assertion +def: "A type of synchrotron radiation circular dichroism evidence that is used in an automatic assertion." [ECO:SN] +synonym: "SRCD" EXACT [] +is_a: ECO:0006178 ! synchrotron radiation circular dichroism evidence +is_a: ECO:0006201 ! circular dichroism evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006204 +name: far-UV circular dichroism evidence used in manual assertion +def: "A type of far-UV circular dichroism evidence that is used in a manual assertion." [ECO:SN] +synonym: "far-UV CD" EXACT [] +is_a: ECO:0006179 ! far-UV circular dichroism evidence +is_a: ECO:0006200 ! circular dichroism evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006205 +name: far-UV circular dichroism evidence used in automatic assertion +def: "A type of far-UV circular dichroism evidence that is used in an automatic assertion." [ECO:SN] +synonym: "far-UV CD" EXACT [] +is_a: ECO:0006179 ! far-UV circular dichroism evidence +is_a: ECO:0006201 ! circular dichroism evidence used in automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006206 +name: near-UV circular dichroism evidence used in manual assertion +def: "A type of near-UV circular dichroism evidence that is used in a manual assertion." [ECO:SN] +synonym: "near-UV CD" EXACT [] +is_a: ECO:0006180 ! near-UV circular dichroism evidence +is_a: ECO:0006200 ! circular dichroism evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006207 +name: near-UV circular dichroism evidence used in automatic assertion +def: "A type of near-UV circular dichroism evidence that is used in an automatic assertion." [ECO:SN] +synonym: "near-UV CD" EXACT [] +is_a: ECO:0006180 ! near-UV circular dichroism evidence +is_a: ECO:0006201 ! circular dichroism evidence used in automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006208 +name: cryogenic electron microscopy evidence used in manual assertion +def: "A type of cryogenic electron microscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "cryo-EM" EXACT [] +is_a: ECO:0006003 ! electron microscopy evidence used in manual assertion +is_a: ECO:0006181 ! cryogenic electron microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006209 +name: cryogenic electron microscopy evidence used in automatic assertion +def: "A type of cryogenic electron microscopy evidence that is used in an automatic assertion." [ECO:SN] +synonym: "cryo-EM" EXACT [] +is_a: ECO:0006181 ! cryogenic electron microscopy evidence +is_a: ECO:0007493 ! electron microscopy evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006210 +name: small-angle X-ray scattering evidence used in manual assertion +def: "A type of small-angle X-ray scattering evidence that is used in a manual assertion." [ECO:SN] +synonym: "SAXS" EXACT [] +is_a: ECO:0006182 ! small-angle X-ray scattering evidence +is_a: ECO:0007062 ! light scattering evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006211 +name: small-angle X-ray scattering evidence used in automatic assertion +def: "A type of small-angle X-ray scattering evidence that is used in an automatic assertion." [ECO:SN] +synonym: "SAXS" EXACT [] +is_a: ECO:0006182 ! small-angle X-ray scattering evidence +is_a: ECO:0007617 ! light scattering evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006212 +name: particle scattering evidence used in manual assertion +def: "A type of particle scattering evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0006183 ! particle scattering evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006213 +name: particle scattering evidence used in automatic assertion +def: "A type of particle scattering evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006183 ! particle scattering evidence +is_a: ECO:0007815 ! structure determination evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006214 +name: small-angle neutron scattering evidence used in manual assertion +def: "A type of small-angle neutron scattering evidence that is used in a manual assertion." [ECO:SN] +synonym: "SANS" EXACT [] +is_a: ECO:0006184 ! small-angle neutron scattering evidence +is_a: ECO:0006212 ! particle scattering evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006215 +name: small-angle neutron scattering evidence used in automatic assertion +def: "A type of small-angle neutron scattering evidence that is used in an automatic assertion." [ECO:SN] +synonym: "SANS" EXACT [] +is_a: ECO:0006184 ! small-angle neutron scattering evidence +is_a: ECO:0006213 ! particle scattering evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006216 +name: author inference used in manual assertion +def: "A type of author inference that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000302 ! author statement used in manual assertion +is_a: ECO:0006185 ! author inference +is_a: ECO:0007751 ! inferential evidence used in manual assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006217 +name: author inference used in automatic assertion +def: "A type of author inference that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006185 ! author inference +is_a: ECO:0007319 ! author statement used in automatic assertion +is_a: ECO:0007832 ! inferential evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006218 +name: combinatorial experimental and author inference evidence contained in single publication used in manual assertion +def: "A type of combinatorial experimental and author inference evidence contained in single publication that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006186 ! combinatorial experimental and author inference evidence contained in single publication +is_a: ECO:0007013 ! combinatorial experimental and author inference evidence used in manual assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006219 +name: combinatorial experimental and author inference evidence contained in single publication used in automatic assertion +def: "A type of combinatorial experimental and author inference evidence contained in single publication that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006186 ! combinatorial experimental and author inference evidence contained in single publication +is_a: ECO:0007592 ! combinatorial experimental and author inference evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006220 +name: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion +def: "A type of X-ray crystallography-based structural model with missing residue coordinates that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005670 ! x-ray crystallography evidence used in manual assertion +is_a: ECO:0006187 ! X-ray crystallography-based structural model with missing residue coordinates +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006221 +name: X-ray crystallography-based structural model with missing residue coordinates used in automatic assertion +def: "A type of X-ray crystallography-based structural model with missing residue coordinates that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0005671 ! x-ray crystallography evidence used in automatic assertion +is_a: ECO:0006187 ! X-ray crystallography-based structural model with missing residue coordinates +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006222 +name: X-ray crystallography-based structural model with high relative B-factor values used in manual assertion +def: "A type of X-ray crystallography-based structural model with high relative B-factor values that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005670 ! x-ray crystallography evidence used in manual assertion +is_a: ECO:0006188 ! X-ray crystallography-based structural model with high relative B-factor values +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006223 +name: X-ray crystallography-based structural model with high relative B-factor values used in automatic assertion +def: "A type of X-ray crystallography-based structural model with high relative B-factor values that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0005671 ! x-ray crystallography evidence used in automatic assertion +is_a: ECO:0006188 ! X-ray crystallography-based structural model with high relative B-factor values +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006224 +name: cryogenic electron microscopy-based structural model with missing residue coordinates used in manual assertion +def: "A type of cryogenic electron microscopy-based structural model with missing residue coordinates that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006189 ! cryogenic electron microscopy-based structural model with missing residue coordinates +is_a: ECO:0006208 ! cryogenic electron microscopy evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006225 +name: cryogenic electron microscopy-based structural model with missing residue coordinates used in automatic assertion +def: "A type of cryogenic electron microscopy-based structural model with missing residue coordinates that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006189 ! cryogenic electron microscopy-based structural model with missing residue coordinates +is_a: ECO:0006209 ! cryogenic electron microscopy evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006226 +name: cryogenic electron microscopy-based structural model with high relative B-factor values used in manual assertion +def: "A type of cryogenic electron microscopy-based structural model with high relative B-factor values that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006190 ! cryogenic electron microscopy-based structural model with high relative B-factor values +is_a: ECO:0006208 ! cryogenic electron microscopy evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006227 +name: cryogenic electron microscopy-based structural model with high relative B-factor values used in automatic assertion +def: "A type of cryogenic electron microscopy-based structural model with high relative B-factor values that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006190 ! cryogenic electron microscopy-based structural model with high relative B-factor values +is_a: ECO:0006209 ! cryogenic electron microscopy evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006228 +name: Fourier-transform infrared spectroscopy evidence used in manual assertion +def: "A type of Fourier-transform infrared spectroscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "FT-IR" EXACT [] +synonym: "FTIR" EXACT [] +is_a: ECO:0001258 ! spectrophotometry evidence used in manual assertion +is_a: ECO:0006191 ! Fourier-transform infrared spectroscopy evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006229 +name: Fourier-transform infrared spectroscopy evidence used in automatic assertion +def: "A type of Fourier-transform infrared spectroscopy evidence that is used in an automatic assertion." [ECO:SN] +synonym: "FT-IR" EXACT [] +synonym: "FTIR" EXACT [] +is_a: ECO:0006191 ! Fourier-transform infrared spectroscopy evidence +is_a: ECO:0007458 ! spectrophotometry evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006230 +name: heat capacity-based evidence used in manual assertion +def: "A type of heat capacity-based evidence that is used in a manual assertion." [ECO:SN] +synonym: "calorimetry evidence" BROAD [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0006192 ! heat capacity-based evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006231 +name: heat capacity-based evidence used in automatic assertion +def: "A type of heat capacity-based evidence that is used in an automatic assertion." [ECO:SN] +synonym: "calorimetry evidence" BROAD [] +is_a: ECO:0006192 ! heat capacity-based evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006232 +name: differential scanning calorimetry evidence used in manual assertion +def: "A type of differential scanning calorimetry evidence that is used in a manual assertion." [ECO:SN] +synonym: "DSC" EXACT [] +is_a: ECO:0006193 ! differential scanning calorimetry evidence +is_a: ECO:0006230 ! heat capacity-based evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006233 +name: differential scanning calorimetry evidence used in automatic assertion +def: "A type of differential scanning calorimetry evidence that is used in an automatic assertion." [ECO:SN] +synonym: "DSC" EXACT [] +is_a: ECO:0006193 ! differential scanning calorimetry evidence +is_a: ECO:0006231 ! heat capacity-based evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006234 +name: selective antibody-based structural conformation evidence used in manual assertion +def: "A type of selective antibody-based structural conformation evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0006194 ! selective antibody-based structural conformation evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006235 +name: selective antibody-based structural conformation evidence used in automatic assertion +def: "A type of selective antibody-based structural conformation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006194 ! selective antibody-based structural conformation evidence +is_a: ECO:0007796 ! immunodetection assay evidence used in automatic assertion +is_a: ECO:0007815 ! structure determination evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006236 +name: protein hydrogen-deuterium exchange mass spectrometry evidence used in manual assertion +def: "A type of protein hydrogen-deuterium exchange mass spectrometry evidence that is used in a manual assertion." [ECO:SN] +synonym: "HDX-MS" EXACT [] +is_a: ECO:0001230 ! mass spectrometry evidence used in manual assertion +is_a: ECO:0006195 ! protein hydrogen-deuterium exchange mass spectrometry evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006237 +name: protein hydrogen-deuterium exchange mass spectrometry evidence used in automatic assertion +def: "A type of protein hydrogen-deuterium exchange mass spectrometry evidence that is used in an automatic assertion." [ECO:SN] +synonym: "HDX-MS" EXACT [] +is_a: ECO:0006195 ! protein hydrogen-deuterium exchange mass spectrometry evidence +is_a: ECO:0007433 ! mass spectrometry evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006238 +name: galactokinase reporter gene assay evidence +def: "A type of reporter gene assay evidence based on the fusion of the galK gene to a specific promoter for the expression of the enzyme galactokinase." [url:https\://www.sciencedirect.com/science/article/pii/007668796608039X] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 OMP:DebbySiegele xsd:string +created_by: snadendla + +[Term] +id: ECO:0006239 +name: polyadenylated transcript 3'-end-sequencing evidence +def: "A type of RNA-sequencing evidence based on sequencing of the 3' ends of cDNA molecules that are selected by presence of a polyadenylated tail." [PMID:31617559] +is_a: ECO:0000295 ! RNA-sequencing evidence +property_value: IAO:0000234 NCBI-RefSeq:MurphyTerence xsd:string +created_by: snadendla + +[Term] +id: ECO:0006240 +name: colony boundary assay evidence +def: "A type of colony morphology phenotypic evidence based on the presence or absence of a visually apparent boundary formed between colonies of multiple isolates or strains of the same microbial species growing on a solid surface such as that of an agar culture medium." [PMID:18621670] +is_a: ECO:0007077 ! colony morphology phenotypic evidence +property_value: IAO:0000234 OMP:DebbySiegele xsd:string +created_by: snadendla + +[Term] +id: ECO:0006241 +name: galactokinase reporter gene assay evidence used in manual assertion +def: "A type of galactokinase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0006238 ! galactokinase reporter gene assay evidence +property_value: ECO:9000002 "IEP" xsd:string +property_value: IAO:0000234 OMP:DebbySiegele xsd:string +created_by: snadendla + +[Term] +id: ECO:0006242 +name: galactokinase reporter gene assay evidence used in automatic assertion +def: "A type of galactokinase reporter gene assay evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006238 ! galactokinase reporter gene assay evidence +is_a: ECO:0007765 ! reporter gene assay evidence used in automatic assertion +property_value: IAO:0000234 OMP:DebbySiegele xsd:string +created_by: snadendla + +[Term] +id: ECO:0006243 +name: polyadenylated transcript 3'-end-sequencing evidence used in manual assertion +def: "A type of polyadenylated transcript 3'-end-sequencing evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006068 ! RNA-sequencing evidence used in manual assertion +is_a: ECO:0006239 ! polyadenylated transcript 3'-end-sequencing evidence +property_value: ECO:9000002 "IEP" xsd:string +property_value: IAO:0000234 NCBI-RefSeq:MurphyTerence xsd:string +created_by: snadendla + +[Term] +id: ECO:0006244 +name: polyadenylated transcript 3'-end-sequencing evidence used in automatic assertion +def: "A type of polyadenylated transcript 3'-end-sequencing evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006069 ! RNA-sequencing evidence used in automatic assertion +is_a: ECO:0006239 ! polyadenylated transcript 3'-end-sequencing evidence +property_value: IAO:0000234 NCBI-RefSeq:MurphyTerence xsd:string +created_by: snadendla + +[Term] +id: ECO:0006245 +name: colony boundary assay evidence used in manual assertion +def: "A type of colony boundary assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006240 ! colony boundary assay evidence +is_a: ECO:0007078 ! colony morphology phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 OMP:DebbySiegele xsd:string +created_by: snadendla + +[Term] +id: ECO:0006246 +name: colony boundary assay evidence used in automatic assertion +def: "A type of colony boundary assay evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006240 ! colony boundary assay evidence +is_a: ECO:0007625 ! colony morphology phenotypic evidence used in automatic assertion +property_value: IAO:0000234 OMP:DebbySiegele xsd:string +created_by: snadendla + +[Term] +id: ECO:0006247 +name: analytical ultracentrifugation evidence +def: "A type of molecule detection assay evidence where the hydrodynamic behaviour of macromolecules is used to study their size, shape and interactions, by spinning the sample solution at high centrifugal field (up to above 100000 rpm and 1000000 g), and monitoring the evolution of sample concentration profile versus the axis of rotation." [PMID:12192063] +synonym: "AUC" EXACT [] +is_a: ECO:0005516 ! molecule detection assay evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006248 +name: fluorescence polarization evidence +def: "A type of physical interaction evidence based on the measurement of the change in the degree of polarization of a fluorophore upon binding to a molecular partner." [PMID:21372817] +synonym: "FP" EXACT [] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006249 +name: obsolete bait-prey protein pull-down evidence +def: "OBSOLETE A type of protein binding evidence resulting from an affinity purification in-vitro technique used to detect physical interactions between two or more proteins, where a molecule of interest - a \"bait\" - is immobilized and incubated with a protein source containing putative \"prey\" proteins." [PMID:28667618] +comment: This term has been obsoleted as this is same as the term bait-prey hybrid interaction evidence. +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +is_obsolete: true +created_by: snadendla + +[Term] +id: ECO:0006250 +name: rotary shadowing electron microscopy evidence +def: "A type of electron microscopy evidence where a heavy metal is evaporated onto surface adsorbed molecules and macromolecular assemblies allowing high-contrast visualization of both individual macromolecules and the surface structure of macromolecular assemblies." [PMID:19247619] +synonym: "RS-TEM" EXACT [] +is_a: ECO:0005033 ! electron microscopy evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006251 +name: electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence +def: "A type of mass spectrometry evidence resulting from the ionization of polar functional groups producing multiple charged ions followed by the detection of ion cyclotron frequencies within a magnetic field, making it suitable for the study of large molecules (>2 kDa)." [PMID:10575730] +synonym: "ESI FT-ICR MS" EXACT [] +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006252 +name: magnetic resonance evidence +def: "A type of experimental evidence in which particles in strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting eletromagnetic signal is captured." [ECO:MG] +is_a: ECO:0000006 ! experimental evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006253 +name: electron paramagnetic resonance evidence +def: "A type of magnetic resonance evidence in which unpaired electrons in a strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting electromagnetic signal is captured." [PMID:21826602, url:https\://en.wikipedia.org/wiki/Electron_paramagnetic_resonance] +synonym: "EPR" EXACT [] +synonym: "ESR" EXACT [] +is_a: ECO:0006252 ! magnetic resonance evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006254 +name: site-directed spin-labelling electron paramagnetic resonance evidence +def: "A type of electron paramagnetic resonance evidence where the signal is obtained by labeling the protein in specific sites with a functional group (most often a nitroxide) containing an unpaired electron using site-directed mutagenesis." [PMID:21826602, url:https\://en.wikipedia.org/wiki/Site-directed_spin_labeling] +synonym: "SDSL" EXACT [] +is_a: ECO:0006253 ! electron paramagnetic resonance evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006255 +name: deglycosylation assay evidence +def: "A type of direct assay evidence where the removal of glycan groups from a substrate (RNA, DNA, or protein) is detected." [url:https\://en.wikipedia.org/wiki/Glycosylation] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006256 +name: protein fragment functional complementation evidence +def: "A type of physical interaction evidence that is based on the detection of a protein-protein interaction between a bait and prey protein by reconstitution of a reporter protein serving as a detectable entity (usually an enzyme or a fluorescent protein), the two halves of which are each attached separately to the bait and prey." [PMID:31274323] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006257 +name: beta galactosidase functional complementation evidence +def: "A type of protein fragment functional complementaion evidence where the interaction between bait and prey assemble a functional beta galactosidase enzyme." [PMID:9237989] +is_a: ECO:0006256 ! protein fragment functional complementation evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006258 +name: GAL4-VP16 functional complementation evidence +def: "A type of protein fragment functional complementaion evidence where the interaction between bait and prey assemble a transcriptional activation complex consisting of the GAL4 DNA-binding domain and the transactivation domain from the herpes simplex virus protein VP16." [PSI-MI:0728] +is_a: ECO:0006256 ! protein fragment functional complementation evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006259 +name: Raman spectroscopy evidence +def: "A type of spectrophotometry evidence used to determine a structural fingerprint of the molecule in solution by probing its vibrational modes via the inelastic scattering of photons (called Raman scattering)." [url:https\://en.wikipedia.org/wiki/Raman_spectroscopy] +synonym: "Raman optical activity" EXACT [] +synonym: "ROA" EXACT [] +is_a: ECO:0001126 ! spectrophotometry evidence +is_a: ECO:0005031 ! structure determination evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006260 +name: protein thermal shift assay evidence +def: "A type of heat capacity-based evidence in which the denaturation temperature of a protein sample is measured under varying conditions, such as pH, redox potential, concentration of other molecules or changes in the proteoform, to determine the effect of these factors on protein thermal stability." [url:https\://en.wikipedia.org/wiki/Thermal_shift_assay] +is_a: ECO:0006192 ! heat capacity-based evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006261 +name: microscale thermophoresis evidence +def: "A type of physical interaction evidence resulting from measuring the change of the fluorescent activity of a molecule as a function of binding to a non-fluorescent partner and its movement in a microspcopic thermal gradient affecting the intensity of fluorescence." [PMID:23270813, url:https\://en.wikipedia.org/wiki/Microscale_thermophoresis] +synonym: "MST" EXACT [] +is_a: ECO:0000021 ! physical interaction evidence +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006262 +name: native protein gel electrophoresis evidence +def: "A type of gel electrophoresis evidence resulting from the use of non-denaturing conditions so that the proteins being measured are in their native structural states." [url:https\://en.wikipedia.org/wiki/Gel_electrophoresis_of_proteins] +synonym: "BN-PAGE" NARROW [] +synonym: "CN-PAGE" NARROW [] +synonym: "native gel" EXACT [] +synonym: "native PAGE" NARROW [] +synonym: "QPNC-PAGE" NARROW [] +is_a: ECO:0000337 ! gel electrophoresis evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006263 +name: turbidity measurement evidence +def: "A type of direct assay evidence where the optical properties of a solution is measured in the visible light spectrum, assessing the haziness or cloudiness as a result of the properties of the solutes or the changes in their material states." [url:https\://en.wikipedia.org/wiki/Turbidity] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006264 +name: competitive binding evidence +def: "A type of affinity evidence derived from an experimental setup where the binding properties of a ligand are assessed by measuring the competition with another ligand with already known binding properties." [PMID:21115850] +synonym: "competition assay" EXACT [] +is_a: ECO:0000023 ! affinity evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006265 +name: protein unfolding evidence +def: "A type of structure determination evidence obtained by monitoring structural changes of a protein as a response to changes of an environmental parameter, such as pH or temperature, or the concentration of a denaturant, such as urea." [url:https\://en.wikipedia.org/wiki/Equilibrium_unfolding] +synonym: "protein denaturation assay" EXACT [] +is_a: ECO:0005031 ! structure determination evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006266 +name: urea-induced protein unfolding evidence +def: "A type of protein unfolding evidence where the unfolding of the protein is triggered by increasing concentrations of urea." [PMID:19708649] +synonym: "chemical denaturation" BROAD [] +synonym: "chemical unfolding" BROAD [] +is_a: ECO:0006265 ! protein unfolding evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006267 +name: pH-induced protein unfolding evidence +def: "A type of protein unfolding evidence where the unfolding of the protein is triggered by changes in pH." [PMID:23185611] +is_a: ECO:0006265 ! protein unfolding evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006268 +name: temperature-induced protein unfolding evidence +def: "A type of protein unfolding evidence where the unfolding of the protein is triggered by changes in temperature." [PMID:23185611] +synonym: "thermal dentauration" EXACT [] +synonym: "thermal unfolding" EXACT [] +is_a: ECO:0006265 ! protein unfolding evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006269 +name: cell aggregation evidence +def: "A type of cell-based assay evidence resulting from the analysis of cells irreversibly binding to each other." [PMID:24092433] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006270 +name: RNA-protein binding evidence +def: "A type of affinity evidence resulting from the qualitative and quantitative analysis of the affinity with which a protein binds to RNA." [PMID:30804549] +is_a: ECO:0000024 ! protein binding evidence +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006271 +name: fluorescence microscopy evidence +def: "A type of microscopy evidence that is obtained by using the fluorescence of the studied sample instead of or in addition to the scattering, reflection, attenuation or absorption of light." [url:https\://en.wikipedia.org/wiki/Fluorescence_microscope] +is_a: ECO:0001098 ! microscopy evidence +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006272 +name: viscosity measurement evidence +def: "A type of direct assay evidence where the viscosity of a solution is measured to quantify the material properties of the solution, or the changes of these properties in response to changing composition or environmental factors." [url:https\://en.wikipedia.org/wiki/Viscometer] +synonym: "viscometry" EXACT [] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006273 +name: dynamic fluorescence quenching evidence +def: "A type of physical interaction evidence derived from the decrease (quenching) of fluorescence as a result of the interaction between a fluorescence donor and acceptor in their excited state." [url:https\://en.wikipedia.org/wiki/Quenching_(fluorescence)] +synonym: "Dexter" NARROW [] +synonym: "exciplex" NARROW [] +synonym: "FRET" NARROW [] +synonym: "quenching" BROAD [] +is_a: ECO:0000021 ! physical interaction evidence +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006274 +name: static fluorescence quenching evidence +def: "A type of physical interaction evidence derived from the decrease (quenching) of fluorescence as a result of the intramolecular interaction between a fluorescence donor and acceptor creating a non-fluorescent ground state." [url:https\://en.wikipedia.org/wiki/Quenching_(fluorescence)] +synonym: "quenching" BROAD [] +is_a: ECO:0000021 ! physical interaction evidence +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006275 +name: analytical ultracentrifugation evidence used in manual assertion +def: "A type of analytical ultracentrifugation evidence that is used in a manual assertion." [ECO:SN] +synonym: "AUC" EXACT [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0006247 ! analytical ultracentrifugation evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006276 +name: analytical ultracentrifugation evidence used in automatic assertion +def: "A type of analytical ultracentrifugation evidence that is used in an automatic assertion." [ECO:SN] +synonym: "AUC" EXACT [] +is_a: ECO:0006247 ! analytical ultracentrifugation evidence +is_a: ECO:0007816 ! molecule detection assay evidence used in automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006277 +name: fluorescence polarization evidence used in manual assertion +def: "A type of fluorescence polarization evidence that is used in a manual assertion." [ECO:SN] +synonym: "FP" EXACT [] +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +is_a: ECO:0006248 ! fluorescence polarization evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006278 +name: fluorescence polarization evidence used in automatic assertion +def: "A type of fluorescence polarization evidence that is used in an automatic assertion." [ECO:SN] +synonym: "FP" EXACT [] +is_a: ECO:0006248 ! fluorescence polarization evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006279 +name: obsolete bait-prey protein pull-down evidence used in manual assertion +def: "OBSOLETE A type of bait-prey protein pull-down evidence that is used in a manual assertion." [ECO:SN] +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +is_obsolete: true +created_by: snadendla + +[Term] +id: ECO:0006280 +name: obsolete bait-prey protein pull-down evidence used in automatic assertion +def: "OBSOLETE A type of bait-prey protein pull-down evidence that is used in an automatic assertion." [ECO:SN] +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +is_obsolete: true +created_by: snadendla + +[Term] +id: ECO:0006281 +name: rotary shadowing electron microscopy evidence used in manual assertion +def: "A type of rotary shadowing electron microscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "RS-TEM" EXACT [] +is_a: ECO:0006003 ! electron microscopy evidence used in manual assertion +is_a: ECO:0006250 ! rotary shadowing electron microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006282 +name: rotary shadowing electron microscopy evidence used in automatic assertion +def: "A type of rotary shadowing electron microscopy evidence that is used in an automatic assertion." [ECO:SN] +synonym: "RS-TEM" EXACT [] +is_a: ECO:0006250 ! rotary shadowing electron microscopy evidence +is_a: ECO:0007493 ! electron microscopy evidence used in automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006283 +name: electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence used in manual assertion +def: "A type of electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence that is used in a manual assertion." [ECO:SN] +synonym: "ESI FT-ICR MS" EXACT [] +is_a: ECO:0001230 ! mass spectrometry evidence used in manual assertion +is_a: ECO:0006251 ! electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006284 +name: electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence used in automatic assertion +def: "A type of electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence that is used in an automatic assertion." [ECO:SN] +synonym: "ESI FT-ICR MS" EXACT [] +is_a: ECO:0006251 ! electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence +is_a: ECO:0007433 ! mass spectrometry evidence used in automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006285 +name: magnetic resonance evidence used in manual assertion +def: "A type of magnetic resonance evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0006252 ! magnetic resonance evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006286 +name: magnetic resonance evidence used in automatic assertion +def: "A type of magnetic resonance evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006252 ! magnetic resonance evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006287 +name: electron paramagnetic resonance evidence used in manual assertion +def: "A type of electron paramagnetic resonance evidence that is used in a manual assertion." [ECO:SN] +synonym: "EPR" EXACT [] +synonym: "ESR" EXACT [] +is_a: ECO:0006253 ! electron paramagnetic resonance evidence +is_a: ECO:0006285 ! magnetic resonance evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006288 +name: electron paramagnetic resonance evidence used in automatic assertion +def: "A type of electron paramagnetic resonance evidence that is used in an automatic assertion." [ECO:SN] +synonym: "EPR" EXACT [] +synonym: "ESR" EXACT [] +is_a: ECO:0006253 ! electron paramagnetic resonance evidence +is_a: ECO:0006286 ! magnetic resonance evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006289 +name: site-directed spin-labelling electron paramagnetic resonance evidence used in manual assertion +def: "A type of site-directed spin-labelling electron paramagnetic resonance evidence that is used in a manual assertion." [ECO:SN] +synonym: "SDSL" EXACT [] +is_a: ECO:0006254 ! site-directed spin-labelling electron paramagnetic resonance evidence +is_a: ECO:0006287 ! electron paramagnetic resonance evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006290 +name: site-directed spin-labelling electron paramagnetic resonance evidence used in automatic assertion +def: "A type of site-directed spin-labelling electron paramagnetic resonance evidence that is used in an automatic assertion." [ECO:SN] +synonym: "SDSL" EXACT [] +is_a: ECO:0006254 ! site-directed spin-labelling electron paramagnetic resonance evidence +is_a: ECO:0006288 ! electron paramagnetic resonance evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006291 +name: deglycosylation assay evidence used in manual assertion +def: "A type of deglycosylation assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0006255 ! deglycosylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006292 +name: deglycosylation assay evidence used in automatic assertion +def: "A type of deglycosylation assay evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006255 ! deglycosylation assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006293 +name: protein fragment functional complementation evidence used in manual assertion +def: "A type of protein fragment functional complementation evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +is_a: ECO:0006256 ! protein fragment functional complementation evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006294 +name: protein fragment functional complementation evidence used in automatic assertion +def: "A type of protein fragment functional complementation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006256 ! protein fragment functional complementation evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006295 +name: beta galactosidase functional complementation evidence used in manual assertion +def: "A type of beta galactosidase functional complementation evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006257 ! beta galactosidase functional complementation evidence +is_a: ECO:0006293 ! protein fragment functional complementation evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006296 +name: beta galactosidase functional complementation evidence used in automatic assertion +def: "A type of beta galactosidase functional complementation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006257 ! beta galactosidase functional complementation evidence +is_a: ECO:0006294 ! protein fragment functional complementation evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006297 +name: GAL4-VP16 functional complementation evidence used in manual assertion +def: "A type of GAL4-VP16 functional complementation evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006258 ! GAL4-VP16 functional complementation evidence +is_a: ECO:0006293 ! protein fragment functional complementation evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006298 +name: GAL4-VP16 functional complementation evidence used in automatic assertion +def: "A type of GAL4-VP16 functional complementation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006258 ! GAL4-VP16 functional complementation evidence +is_a: ECO:0006294 ! protein fragment functional complementation evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006299 +name: Raman spectroscopy evidence used in manual assertion +def: "A type of Raman spectroscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "Raman optical activity" EXACT [] +synonym: "ROA" EXACT [] +is_a: ECO:0001258 ! spectrophotometry evidence used in manual assertion +is_a: ECO:0006259 ! Raman spectroscopy evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006300 +name: Raman spectroscopy evidence used in automatic assertion +def: "A type of Raman spectroscopy evidence that is used in an automatic assertion." [ECO:SN] +synonym: "Raman optical activity" EXACT [] +synonym: "ROA" EXACT [] +is_a: ECO:0006259 ! Raman spectroscopy evidence +is_a: ECO:0007458 ! spectrophotometry evidence used in automatic assertion +is_a: ECO:0007815 ! structure determination evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006301 +name: protein thermal shift assay evidence used in manual assertion +def: "A type of protein thermal shift assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006230 ! heat capacity-based evidence used in manual assertion +is_a: ECO:0006260 ! protein thermal shift assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006302 +name: protein thermal shift assay evidence used in automatic assertion +def: "A type of protein thermal shift assay evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006231 ! heat capacity-based evidence used in automatic assertion +is_a: ECO:0006260 ! protein thermal shift assay evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006303 +name: microscale thermophoresis evidence used in manual assertion +def: "A type of microscale thermophoresis evidence that is used in a manual assertion." [ECO:SN] +synonym: "MST" EXACT [] +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +is_a: ECO:0001249 ! fluorescence evidence used in manual assertion +is_a: ECO:0006261 ! microscale thermophoresis evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006304 +name: microscale thermophoresis evidence used in automatic assertion +def: "A type of microscale thermophoresis evidence that is used in an automatic assertion." [ECO:SN] +synonym: "MST" EXACT [] +is_a: ECO:0006261 ! microscale thermophoresis evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +is_a: ECO:0007450 ! fluorescence evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006305 +name: native protein gel electrophoresis evidence used in manual assertion +def: "A type of native protein gel electrophoresis evidence that is used in a manual assertion." [ECO:SN] +synonym: "BN-PAGE" NARROW [] +synonym: "CN-PAGE" NARROW [] +synonym: "native gel" EXACT [] +synonym: "native PAGE" NARROW [] +synonym: "QPNC-PAGE" NARROW [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0006262 ! native protein gel electrophoresis evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006306 +name: native protein gel electrophoresis evidence used in automatic assertion +def: "A type of native protein gel electrophoresis evidence that is used in an automatic assertion." [ECO:SN] +synonym: "BN-PAGE" NARROW [] +synonym: "CN-PAGE" NARROW [] +synonym: "native gel" EXACT [] +synonym: "native PAGE" NARROW [] +synonym: "QPNC-PAGE" NARROW [] +is_a: ECO:0006262 ! native protein gel electrophoresis evidence +is_a: ECO:0007769 ! gel electrophoresis evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006307 +name: turbidity measurement evidence used in manual assertion +def: "A type of turbidity measurement evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0006263 ! turbidity measurement evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006308 +name: turbidity measurement evidence used in automatic assertion +def: "A type of turbidity measurement evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006263 ! turbidity measurement evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006309 +name: competitive binding evidence used in manual assertion +def: "A type of competitive binding evidence that is used in a manual assertion." [ECO:SN] +synonym: "competition assay" EXACT [] +is_a: ECO:0006075 ! affinity evidence used in manual assertion +is_a: ECO:0006264 ! competitive binding evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006310 +name: competitive binding evidence used in automatic assertion +def: "A type of competitive binding evidence that is used in an automatic assertion." [ECO:SN] +synonym: "competition assay" EXACT [] +is_a: ECO:0006264 ! competitive binding evidence +is_a: ECO:0007384 ! affinity evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006311 +name: protein unfolding evidence used in manual assertion +def: "A type of protein unfolding evidence that is used in a manual assertion." [ECO:SN] +synonym: "protein denaturation assay" EXACT [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0006265 ! protein unfolding evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006312 +name: protein unfolding evidence used in automatic assertion +def: "A type of protein unfolding evidence that is used in an automatic assertion." [ECO:SN] +synonym: "protein denaturation assay" EXACT [] +is_a: ECO:0006265 ! protein unfolding evidence +is_a: ECO:0007815 ! structure determination evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006313 +name: urea-induced protein unfolding evidence used in manual assertion +def: "A type of urea-induced protein unfolding evidence that is used in a manual assertion." [ECO:SN] +synonym: "chemical denaturation" BROAD [] +synonym: "chemical unfolding" BROAD [] +is_a: ECO:0006266 ! urea-induced protein unfolding evidence +is_a: ECO:0006311 ! protein unfolding evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006314 +name: urea-induced protein unfolding evidence used in automatic assertion +def: "A type of urea-induced protein unfolding evidence that is used in an automatic assertion." [ECO:SN] +synonym: "chemical denaturation" BROAD [] +synonym: "chemical unfolding" BROAD [] +is_a: ECO:0006266 ! urea-induced protein unfolding evidence +is_a: ECO:0006312 ! protein unfolding evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006315 +name: pH-induced protein unfolding evidence used in manual assertion +def: "A type of pH-induced protein unfolding evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006267 ! pH-induced protein unfolding evidence +is_a: ECO:0006311 ! protein unfolding evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006316 +name: pH-induced protein unfolding evidence used in automatic assertion +def: "A type of pH-induced protein unfolding evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006267 ! pH-induced protein unfolding evidence +is_a: ECO:0006312 ! protein unfolding evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006317 +name: temperature-induced protein unfolding evidence used in manual assertion +def: "A type of temperature-induced protein unfolding evidence that is used in a manual assertion." [ECO:SN] +synonym: "thermal dentauration" EXACT [] +synonym: "thermal unfolding" EXACT [] +is_a: ECO:0006268 ! temperature-induced protein unfolding evidence +is_a: ECO:0006311 ! protein unfolding evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006318 +name: temperature-induced protein unfolding evidence used in automatic assertion +def: "A type of temperature-induced protein unfolding evidence that is used in an automatic assertion." [ECO:SN] +synonym: "thermal dentauration" EXACT [] +synonym: "thermal unfolding" EXACT [] +is_a: ECO:0006268 ! temperature-induced protein unfolding evidence +is_a: ECO:0006312 ! protein unfolding evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006319 +name: cell aggregation evidence used in manual assertion +def: "A type of cell aggregation evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0006269 ! cell aggregation evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006320 +name: cell aggregation evidence used in automatic assertion +def: "A type of cell aggregation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006269 ! cell aggregation evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006321 +name: RNA-protein binding evidence used in manual assertion +def: "A type of RNA-protein binding evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006076 ! protein binding evidence used in manual assertion +is_a: ECO:0006083 ! nucleic acid binding evidence used in manual assertion +is_a: ECO:0006270 ! RNA-protein binding evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006322 +name: RNA-protein binding evidence used in automatic assertion +def: "A type of RNA-protein binding evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006270 ! RNA-protein binding evidence +is_a: ECO:0007373 ! protein binding evidence used in automatic assertion +is_a: ECO:0007505 ! nucleic acid binding evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006323 +name: fluorescence microscopy evidence used in manual assertion +def: "A type of fluorescence microscopy evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001232 ! microscopy evidence used in manual assertion +is_a: ECO:0001249 ! fluorescence evidence used in manual assertion +is_a: ECO:0006271 ! fluorescence microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006324 +name: fluorescence microscopy evidence used in automatic assertion +def: "A type of fluorescence microscopy evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006271 ! fluorescence microscopy evidence +is_a: ECO:0007435 ! microscopy evidence used in automatic assertion +is_a: ECO:0007450 ! fluorescence evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006325 +name: viscosity measurement evidence used in manual assertion +def: "A type of viscosity measurement evidence that is used in a manual assertion." [ECO:SN] +synonym: "viscometry" EXACT [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0006272 ! viscosity measurement evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006326 +name: viscosity measurement evidence used in automatic assertion +def: "A type of viscosity measurement evidence that is used in an automatic assertion." [ECO:SN] +synonym: "viscometry" EXACT [] +is_a: ECO:0006272 ! viscosity measurement evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006327 +name: dynamic fluorescence quenching evidence used in manual assertion +def: "A type of dynamic fluorescence quenching evidence that is used in a manual assertion." [ECO:SN] +synonym: "Dexter" NARROW [] +synonym: "exciplex" NARROW [] +synonym: "FRET" NARROW [] +synonym: "quenching" BROAD [] +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +is_a: ECO:0001249 ! fluorescence evidence used in manual assertion +is_a: ECO:0006273 ! dynamic fluorescence quenching evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006328 +name: dynamic fluorescence quenching evidence used in automatic assertion +def: "A type of dynamic fluorescence quenching evidence that is used in an automatic assertion." [ECO:SN] +synonym: "Dexter" NARROW [] +synonym: "exciplex" NARROW [] +synonym: "FRET" NARROW [] +synonym: "quenching" BROAD [] +is_a: ECO:0006273 ! dynamic fluorescence quenching evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +is_a: ECO:0007450 ! fluorescence evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006329 +name: static fluorescence quenching evidence used in manual assertion +def: "A type of static fluorescence quenching evidence that is used in a manual assertion." [ECO:SN] +synonym: "quenching" BROAD [] +is_a: ECO:0000353 ! physical interaction evidence used in manual assertion +is_a: ECO:0001249 ! fluorescence evidence used in manual assertion +is_a: ECO:0006274 ! static fluorescence quenching evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006330 +name: static fluorescence quenching evidence used in automatic assertion +def: "A type of static fluorescence quenching evidence that is used in an automatic assertion." [ECO:SN] +synonym: "quenching" BROAD [] +is_a: ECO:0006274 ! static fluorescence quenching evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +is_a: ECO:0007450 ! fluorescence evidence used in automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0007000 +name: high throughput mutant phenotypic evidence +def: "A type of high throughput evidence derived from high throughput analysis of differences in alleles of a corresponding gene." [GO:IMP] +synonym: "high throughput mutant phenotype evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +is_a: ECO:0006055 ! high throughput evidence +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007001 +name: high throughput mutant phenotypic evidence used in manual assertion +def: "A type of high throughput mutant phenotypic evidence that is used in a manual assertion." [GO:IMP] +comment: When using the HMP evidence code, the guidelines for IMP should be adhered to (http://geneontology.org/page/imp-inferred-mutant-phenotype) +synonym: "high throughput mutant phenotype evidence used in manual assertion" EXACT [] +synonym: "HMP" EXACT [GOECO:HMP] +synonym: "inferred from high throughput mutant phenotype" EXACT [GOECO:HMP] +xref: GOECO:HMP "inferred from high throughput mutant phenotype" +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0006056 ! high throughput evidence used in manual assertion +is_a: ECO:0007000 ! high throughput mutant phenotypic evidence +property_value: ECO:9000002 "HMP" xsd:string {comment="Default"} +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007002 +name: high throughput genetic interaction phenotypic evidence +def: "A type of high throughput evidence resulting from the high throughput analysis of the effect that a given gene has on another gene or genes, and products." [PMID:11822023] +synonym: "high throughput genetic interaction evidence" EXACT [] +is_a: ECO:0000011 ! genetic interaction evidence +is_a: ECO:0006055 ! high throughput evidence +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007003 +name: high throughput genetic interaction phenotypic evidence used in manual assertion +def: "A type of high throughput genetic interaction phenotypic evidence that is used in a manual assertion." [PMID:11822023] +comment: When using the HGI evidence code, the guidelines for IGI should be adhered to (http://geneontology.org/page/igi-inferred-genetic-interaction) +synonym: "HGI" EXACT [GOECO:HGI] +synonym: "high throughput genetic interaction evidence used in manual assertion" EXACT [] +synonym: "inferred from high throughput genetic interaction" EXACT [GOECO:HGI] +xref: GOECO:HGI "inferred from high throughput genetic interaction" +is_a: ECO:0000316 ! genetic interaction evidence used in manual assertion +is_a: ECO:0006056 ! high throughput evidence used in manual assertion +is_a: ECO:0007002 ! high throughput genetic interaction phenotypic evidence +property_value: ECO:9000002 "HGI" xsd:string {comment="Default"} +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007004 +name: high throughput direct assay evidence +def: "A type of high throughput evidence derived from the high throughput direct measurement of some aspect of a biological feature." [ECO:MCC] +is_a: ECO:0000002 ! direct assay evidence +is_a: ECO:0006055 ! high throughput evidence +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007005 +name: high throughput direct assay evidence used in manual assertion +def: "A type of high throughput evidence that is used in a manual assertion derived from the high throughput direct measurement of some aspect of a biological feature." [ECO:MCC] +comment: When using the HDA evidence code, the guidelines for IDA should be adhered to (http://geneontology.org/page/ida-inferred-direct-assay) +synonym: "HDA" EXACT [GOECO:HDA] +synonym: "inferred from high throughput direct assay" EXACT [GOECO:HDA] +xref: GOECO:HDA "inferred from high throughput direct assay" +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0006056 ! high throughput evidence used in manual assertion +is_a: ECO:0007004 ! high throughput direct assay evidence +property_value: ECO:9000002 "HDA" xsd:string {comment="Default"} +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007006 +name: high throughput expression pattern evidence +def: "A type of high throughput evidence derived from the high throughput characterization of gene expression." [GO:IEP] +is_a: ECO:0000008 ! expression pattern evidence +is_a: ECO:0006055 ! high throughput evidence +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007007 +name: high throughput expression pattern evidence used in manual assertion +def: "A type of high throughput evidence that is used in a manual assertion derived from the high throughput characterization of gene expression." [GO:IEP] +comment: When using the HEP evidence code, the guidelines for EXP should be adhered to (http://geneontology.org/page/iep-inferred-expression-pattern) +synonym: "HEP" EXACT [GOECO:HEP] +synonym: "inferred from high throughput expression pattern" EXACT [GOECO:HEP] +xref: GOECO:HEP "inferred from high throughput expression pattern" +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0006056 ! high throughput evidence used in manual assertion +is_a: ECO:0007006 ! high throughput expression pattern evidence +property_value: ECO:9000002 "HEP" xsd:string {comment="Default"} +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007008 +name: radioligand binding assay evidence +def: "A type of protein binding evidence in which radioactive ligands are used to measure receptor-ligand interactions, such as in determining selectivity for a particular ligand." [PMID:27471749] +synonym: "competitive binding assay evidence" NARROW [] +synonym: "radioactive ligand binding assay evidence" EXACT [] +synonym: "radiometric ligand-binding assay evidence" EXACT [] +is_a: ECO:0000024 ! protein binding evidence +property_value: IAO:0000234 "fjungo" xsd:string +created_by: rctauber +creation_date: 2017-12-15T10:46:19Z + +[Term] +id: ECO:0007009 +name: radioligand binding assay evidence used in manual assertion +def: "A type of radioligand binding assay evidence that is used in a manual assertion." [ECO:RCT] +synonym: "competitive binding assay evidence" RELATED [] +synonym: "radioactive ligand binding assay evidence" BROAD [] +is_a: ECO:0006076 ! protein binding evidence used in manual assertion +is_a: ECO:0007008 ! radioligand binding assay evidence +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-12-15T10:48:44Z + +[Term] +id: ECO:0007011 +name: combinatorial experimental and author inference evidence +def: "A type of combinatorial evidence in which the author draws a conclusion based on a combination of prior scientific background knowledge and evidence from one or more experiments." [ECO:RCT] +synonym: "combinatorial evidence from author knowledge and experimental evidence" EXACT [] +is_a: ECO:0000212 ! combinatorial evidence +property_value: IAO:0000234 "sbello" xsd:string +created_by: rctauber +creation_date: 2018-02-15T11:17:11Z + +[Term] +id: ECO:0007012 +name: combinatorial experimental and curator inference evidence +def: "A type of combinatorial evidence in which the curator draws a conclusion based on a combination of prior scientific background knowledge and evidence from one or more experiments." [ECO:RCT] +synonym: "combinatorial evidence from curator knowledge and experimental evidence" EXACT [] +is_a: ECO:0000212 ! combinatorial evidence +property_value: IAO:0000234 "ZFIN" xsd:string +created_by: rctauber +creation_date: 2018-02-15T11:17:11Z + +[Term] +id: ECO:0007013 +name: combinatorial experimental and author inference evidence used in manual assertion +def: "A type of combinatorial evidence from author knowledge and experimental evidence that is used in a manual assertion." [ECO:RCT] +synonym: "combinatorial evidence from author knowledge and experimental evidence used in manual assertion" EXACT [] +is_a: ECO:0000244 ! combinatorial evidence used in manual assertion +is_a: ECO:0007011 ! combinatorial experimental and author inference evidence +created_by: rctauber +creation_date: 2018-02-15T11:22:10Z + +[Term] +id: ECO:0007014 +name: combinatorial experimental and curator inference evidence used in manual assertion +def: "A type of combinatorial evidence from curator knowledge and experimental evidence that is used in a manual assertion." [ECO:RCT] +synonym: "combinatorial evidence from curator knowledge and experimental evidence used in manual assertion" EXACT [] +is_a: ECO:0000244 ! combinatorial evidence used in manual assertion +is_a: ECO:0007012 ! combinatorial experimental and curator inference evidence +created_by: rctauber +creation_date: 2018-02-15T11:22:10Z + +[Term] +id: ECO:0007015 +name: voltammetry evidence +def: "A type of substance quantification evidence where an electrical gradient potential is applied to analyze an analyte of interest, resulting in a measurement amount of electrical current across the potential range applied." [PMID:28127962] +is_a: ECO:0000335 ! substance quantification evidence +property_value: IAO:0000234 "SynGO" xsd:string +created_by: rctauber +creation_date: 2018-02-28T12:11:00Z + +[Term] +id: ECO:0007016 +name: voltammetry evidence used in manual assertion +def: "A type of voltammetry evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0007015 ! voltammetry evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-02-28T12:12:34Z + +[Term] +id: ECO:0007017 +name: photoconversion evidence +def: "A type of fluorescence evidence where a molecule tagged with a fluorescent protein is exposed to ultraviolet or blue light to shift the spectral emission properties for visualization." [ECO:RCT, PMID:28574633] +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000234 "SynGO" xsd:string +created_by: rctauber +creation_date: 2018-03-02T09:01:00Z + +[Term] +id: ECO:0007018 +name: photoconversion evidence used in manual assertion +def: "A type of photoconversion evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001249 ! fluorescence evidence used in manual assertion +is_a: ECO:0007017 ! photoconversion evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-03-02T09:05:04Z + +[Term] +id: ECO:0007019 +name: agglutination test evidence +def: "A type of immunological assay evidence that involves observation of visible clumping of antibody and antigen into a complex." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC380037/] +synonym: "agglutination assay evidence" EXACT [] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007020 +name: agglutination test evidence used in manual assertion +def: "A type of agglutination test evidence that is used in a manual assertion." [ECO:RCT] +synonym: "agglutination assay evidence" BROAD [] +is_a: ECO:0006078 ! immunological assay evidence used in manual assertion +is_a: ECO:0007019 ! agglutination test evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007021 +name: slide agglutination test evidence +def: "A type of agglutination test evidence that involves observaiton of visible clumping of antibody and antigen into a complex on a slide." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC380037/] +is_a: ECO:0007019 ! agglutination test evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007022 +name: slide agglutination test evidence used in manual assertion +def: "A type of slide agglutination test evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0007020 ! agglutination test evidence used in manual assertion +is_a: ECO:0007021 ! slide agglutination test evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007023 +name: direct Coombs test evidence +def: "A type of agglutination test evidence that results in detection of nonagglutinating antibodies or complement proteins on red blood cells in vivo." [URL:https\://courses.lumenlearning.com/microbiology/chapter/agglutination-assays/] +synonym: "direct antihuman globulin test (DAT)" EXACT [] +is_a: ECO:0007019 ! agglutination test evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007024 +name: direct Coombs test evidence used in manual assertion +def: "A type of direct Coombs test evidence that is used in a manual assertion." [ECO:RCT] +synonym: "direct antihuman globulin test (DAT)" BROAD [] +is_a: ECO:0007020 ! agglutination test evidence used in manual assertion +is_a: ECO:0007023 ! direct Coombs test evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007025 +name: indirect Coombs test evidence +def: "A type of agglutination test evidence that results in screening of antibodies against red blood cell antigens (other than the A and B antigens) that are unbound in a patient's serum in vitro." [URL:https\://courses.lumenlearning.com/microbiology/chapter/agglutination-assays/] +synonym: "indirect antiglobulin test (IAT)" EXACT [] +is_a: ECO:0007019 ! agglutination test evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007026 +name: indirect Coombs test evidence used in manual assertion +def: "A type of indirect Coombs test evidence that is used in a manual assertion." [ECO:RCT] +synonym: "indirect antiglobulin test (IAT)" BROAD [] +is_a: ECO:0007020 ! agglutination test evidence used in manual assertion +is_a: ECO:0007025 ! indirect Coombs test evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007027 +name: direct hemagglutination assay evidence +def: "A type of agglutination test evidence where some bacteria and viruses cross-link red blood cells and clump together." [URL:https\://courses.lumenlearning.com/microbiology/chapter/agglutination-assays/] +is_a: ECO:0007019 ! agglutination test evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007028 +name: direct hemagglutination assay evidence used in manual assertion +def: "A type of direct hemagglutination assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0007020 ! agglutination test evidence used in manual assertion +is_a: ECO:0007027 ! direct hemagglutination assay evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007029 +name: viral hemagglutination inhibition assay evidence +def: "A type of agglutination test evidence where antiviral antibodies in a patient's serum or in a lab-produced antiserum neutralize the virus and block it from agglutinating the red blood cells." [URL:https\://courses.lumenlearning.com/microbiology/chapter/agglutination-assays/] +is_a: ECO:0007019 ! agglutination test evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007030 +name: viral hemagglutination inhibition assay evidence used in manual assertion +def: "A type of viral hemagglutination inhibition assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0007020 ! agglutination test evidence used in manual assertion +is_a: ECO:0007029 ! viral hemagglutination inhibition assay evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007031 +name: compement fixation assay evidence +def: "A type of immunological assay evidence that results in the detection of presence of specific antibodies in the patient's serum based on the use of complement, a biologically labile serum factor." [URL:http\://laboratoryinfo.com/complement-fixation-test/] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007032 +name: compement fixation assay evidence used in manual assertion +def: "A type of compement fixation assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006078 ! immunological assay evidence used in manual assertion +is_a: ECO:0007031 ! compement fixation assay evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007033 +name: neutralization test assay evidence +def: "A type of immunogical assay evidence that results in detection of specific neutralizing antibodies in a sample." [PMID:24899440, url:http\://www.creative-biolabs.com/drug-discovery/therapeutics/neutralization-assay.htm?gclid=Cj0KEQjwqvvLBRDIt-D7q7iqiOcBEiQAxi68ERRz00a0x9sWSKSLDu7d5-kMi4AdiLNdUq1mXzePdywaArli8P8HAQ)] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007034 +name: neutralization test assay evidence used in manual assertion +def: "A type of neutralization test assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006078 ! immunological assay evidence used in manual assertion +is_a: ECO:0007033 ! neutralization test assay evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007035 +name: copper transport assay evidence +def: "A type of transport assay evidence where the rate of copper transport is estimated." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689948/] +is_a: ECO:0000134 ! transport assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007036 +name: copper transport assay evidence used in manual assertion +def: "A type of copper transport assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0007035 ! copper transport assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007037 +name: 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence +def: "A type of staining evidence that involves the use of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride to evaluate the respiratory activity of cells." [PMID:1622256] +synonym: "CTC staining" EXACT [] +is_a: ECO:0005019 ! staining evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007038 +name: 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence used in manual assertion +def: "A type of 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence that is used in a manual assertion." [ECO:RCT] +synonym: "CTC staining" BROAD [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0007037 ! 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007039 +name: plaque assay evidence +def: "A type of cell growth assay evidence resulting in direct quantification of infectious virons and antiviral substances through the counting of discrete plaques (infectious units and cellular dead zones) in cell culture." [PMID:25407402] +is_a: ECO:0001563 ! cell growth assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007040 +name: plaque assay evidence used in manual assertion +def: "A type of plaque assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0007039 ! plaque assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007041 +name: epifluorescence microscopy evidence +def: "A type of fluorescence microscopy evidence where the light source is mounted above the specimen and the excitation light passes through the microscope objective lens on its way toward the specimen." [URL:http\://www.rsc.org/publishing/journals/prospect/ontology.asp?id=CMO\:0001096|URL\:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713126/] +is_a: ECO:0006271 ! fluorescence microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007042 +name: epifluorescence microscopy evidence used in manual assertion +def: "A type of epifluorescence microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006323 ! fluorescence microscopy evidence used in manual assertion +is_a: ECO:0007041 ! epifluorescence microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007043 +name: transmission electron microscopy evidence +def: "A type of electron microscopy evidence where a beam of electrons are transmitted through an ultra thin specimen forming an image which is magnified and focused onto an imaging device, such as a fluorescent screen, on a layer of photographic film, or to be detected by a sensor such as a CCD camera." [ERO:0000329|URL\:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907272/] +synonym: "conventional transmission electron microscopy(CTEM)" EXACT [] +synonym: "TEM" EXACT [] +is_a: ECO:0005033 ! electron microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007044 +name: transmission electron microscopy evidence used in manual assertion +def: "A type of transmission electron microscopy evidence that is used in a manual assertion." [ECO:RCT] +synonym: "conventional transmission electron microscopy(CTEM)" BROAD [] +synonym: "TEM" BROAD [] +is_a: ECO:0006003 ! electron microscopy evidence used in manual assertion +is_a: ECO:0007043 ! transmission electron microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007045 +name: scanning electron microscopy evidence +def: "A type of electron microscopy evidence where a focused beam of high-energy electrons is used to generate a variety of signals at the surface of solid specimens." [URL:https\://serc.carleton.edu/research_education/geochemsheets/techniques/SEM.html] +is_a: ECO:0005033 ! electron microscopy evidence +property_value: IAO:0000112 "With a general growth of the recombinant mycobacterial strains resulting in minimal change, the cell morphology was further examined using the scanning electron microscopy (SEM) technique. As shown in Fig. 5B, the cell lengthened when 20 ng/mL tetracycline was added to the medium to induce expression of the antisense mtrA mRNA (right panel)." xsd:string {xref="PMID:20843371"} +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007046 +name: scanning electron microscopy evidence used in manual assertion +def: "A type of scanning electron microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006003 ! electron microscopy evidence used in manual assertion +is_a: ECO:0007045 ! scanning electron microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007047 +name: time-lapsed microscopy evidence +def: "A type of microscopy evidence where a serial images are taken at regular time points to capture the dynamics of what is being observed." [URL:https\://www.nature.com/subjects/time-lapse-imaging] +is_a: ECO:0001098 ! microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007048 +name: time-lapsed microscopy evidence used in manual assertion +def: "A type of time-lapsed microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001232 ! microscopy evidence used in manual assertion +is_a: ECO:0007047 ! time-lapsed microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007049 +name: phase contrast microscopy evidence +def: "A type of microscopy evidence where a transparent specimen is illuminated with visible light and small phase shifts in the light passing through the specimen are used to produce an image." [CHMO:0000110] +is_a: ECO:0001098 ! microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007050 +name: phase contrast microscopy evidence used in manual assertion +def: "A type of phase contrast microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001232 ! microscopy evidence used in manual assertion +is_a: ECO:0007049 ! phase contrast microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007051 +name: transmitted light brightfied mircoscopy evidence +def: "A type of microscopy evidence where a sample is illuminated with transmitted white light from below and observed from above." [BAO:0000457] +is_a: ECO:0001098 ! microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007052 +name: transmitted light brightfied mircoscopy evidence used in manual assertion +def: "A type of transmitted light brightfied mircoscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001232 ! microscopy evidence used in manual assertion +is_a: ECO:0007051 ! transmitted light brightfied mircoscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007053 +name: koehler illumination microscopy evidence +def: "A type of microscopy evidence that results in a bright image without glare and minimum heating of the specimen by employing both field and an aperture iris diaphragm for illumination." [URL:http\://www.gonda.ucla.edu/bri_core/kohler.htm] +is_a: ECO:0001098 ! microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007054 +name: koehler illumination microscopy evidence used in manual assertion +def: "A type of koehler illumination microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001232 ! microscopy evidence used in manual assertion +is_a: ECO:0007053 ! koehler illumination microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007055 +name: differential interference contrast microscopy evidence +def: "A type of microscopy evidence that results in visualization of living cells and transparent specimens by taking advantage of differences in the light refraction of different parts of the specimen." [URL:http\://www.microscopemaster.com/differential-interference-contrast.html] +synonym: "DIC microscopy" EXACT [] +synonym: "NIC microscopy" EXACT [] +synonym: "Nomarski interference contrast microscopy" EXACT [] +synonym: "Nomarski microscopy" EXACT [] +is_a: ECO:0001098 ! microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007056 +name: differential interference contrast microscopy evidence used in manual assertion +def: "A type of differential interference contrast microscopy evidence that is used in a manual assertion." [ECO:RCT] +synonym: "DIC microscopy" BROAD [] +synonym: "NIC microscopy" BROAD [] +synonym: "Nomarski interference contrast microscopy" BROAD [] +synonym: "Nomarski microscopy" BROAD [] +is_a: ECO:0001232 ! microscopy evidence used in manual assertion +is_a: ECO:0007055 ! differential interference contrast microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007057 +name: extended field laser confocal microscopy evidence +def: "A type of confocal microscopy evidence that results in creation of a continuous confocal multi-colour mosaic from thousands of individually captured images." [PMID:18627634] +synonym: "EFLCM" EXACT [] +is_a: ECO:0005587 ! confocal microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007058 +name: extended field laser confocal microscopy evidence used in manual assertion +def: "A type of extended field laser confocal microscopy evidence that is used in a manual assertion." [ECO:RCT] +synonym: "EFLCM" BROAD [] +is_a: ECO:0005589 ! confocal microscopy evidence used in manual assertion +is_a: ECO:0007057 ! extended field laser confocal microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007059 +name: confocal laser scanning microscopy evidence +def: "A type of confocal microscopy evidence that results in an image that is built up pixel-by-pixel by collecting the emitted photons from the fluorophores in the sample by passing a laser beam through a light source aperture which is then focused by an objective lens into a small area on the surface of the sample.\n" [URL:http\://bitesizebio.com/19958/what-is-confocal-laser-scanning-microscopy/] +synonym: "CLSM" EXACT [] +synonym: "confocal laser scanning fluorescence microscopy" EXACT [] +synonym: "confocal-laser scanning microscopy" EXACT [] +synonym: "fluorescence confocal scanning laser microscopy" EXACT [] +synonym: "LSCM" EXACT [] +synonym: "scanning confocal fluorescence microscopy" EXACT [] +is_a: ECO:0005587 ! confocal microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007060 +name: confocal laser scanning microscopy evidence used in manual assertion +def: "A type of confocal laser scanning microscopy evidence that is used in a manual assertion." [ECO:RCT] +synonym: "CLSM" BROAD [] +synonym: "confocal laser scanning fluorescence microscopy" BROAD [] +synonym: "confocal-laser scanning microscopy" BROAD [] +synonym: "fluorescence confocal scanning laser microscopy" BROAD [] +synonym: "LSCM" BROAD [] +synonym: "scanning confocal fluorescence microscopy" BROAD [] +is_a: ECO:0005589 ! confocal microscopy evidence used in manual assertion +is_a: ECO:0007059 ! confocal laser scanning microscopy evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007061 +name: light scattering evidence +def: "A type of structure determination evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight, diffusion and interaction strength by illuminating the sample with a laser beam and the scattered intensity is probed at a certain angle by a detector." [PMID:9013660, url:http\://www.lsinstruments.ch/technology/dynamic_light_scattering_dls/, url:http\://www.soft-matter.uni-tuebingen.de/index.html?dls.html] +is_a: ECO:0005031 ! structure determination evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007062 +name: light scattering evidence used in manual assertion +def: "A type of light scattering evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0007061 ! light scattering evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007063 +name: dynamic light scattering assay evidence +def: "A type of light scattering evidence derived by analyzing the fluctuations in the internsity of the scattered light by the particles in motion." [PMID:9013660, url:http\://www.soft-matter.uni-tuebingen.de/index.html?dls.html] +synonym: "Photon Correlation Spectroscopy" EXACT [] +synonym: "Quasi-Elastic Light Scattering" EXACT [] +is_a: ECO:0007061 ! light scattering evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007064 +name: dynamic light scattering assay evidence used in manual assertion +def: "A type of dynamic light scattering assay evidence that is used in a manual assertion." [ECO:RCT] +synonym: "Photon Correlation Spectroscopy" BROAD [] +synonym: "Quasi-Elastic Light Scattering" BROAD [] +is_a: ECO:0007062 ! light scattering evidence used in manual assertion +is_a: ECO:0007063 ! dynamic light scattering assay evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007065 +name: static light scattering assay evidence +def: "A type of light scattering evidence derived by measuring the average intensity of scattered light by the particles at multiple angles." [PMID:9013660] +synonym: "Rayleigh scattering" EXACT [] +is_a: ECO:0007061 ! light scattering evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007066 +name: static light scattering assay evidence used in manual assertion +def: "A type of static light scattering assay evidence that is used in a manual assertion." [ECO:RCT] +synonym: "Rayleigh scattering" BROAD [] +is_a: ECO:0007062 ! light scattering evidence used in manual assertion +is_a: ECO:0007065 ! static light scattering assay evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007067 +name: colony papillation assay phenotypic evidence +def: "A type of colony morphology phenotypic evidence resulting from the formation of papillae (microcolonies) that protrude outwards from the main colony by mutant cells." [PMID:27447898] +synonym: "colony papillation assay evidence" EXACT [] +is_a: ECO:0007077 ! colony morphology phenotypic evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007068 +name: colony papillation assay phenotypic evidence used in manual assertion +def: "A type of colony papillation assay phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "colony papillation assay evidence used in manual assertion" EXACT [] +is_a: ECO:0007067 ! colony papillation assay phenotypic evidence +is_a: ECO:0007078 ! colony morphology phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007069 +name: crystal violet staining evidence +def: "A type of staining evidence that is derived from the use of gentian violet as a general biological stain and an acid-base indicator." [BAO:0002468] +synonym: "gentian violet" EXACT [] +synonym: "hexamethyl pararosaniline chloride" EXACT [] +synonym: "methyl violet 10B" EXACT [] +is_a: ECO:0005019 ! staining evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007070 +name: crystal violet staining evidence used in manual assertion +def: "A type of crystal violet staining evidence that is used in a manual assertion." [ECO:RCT] +synonym: "gentian violet" BROAD [] +synonym: "hexamethyl pararosaniline chloride" BROAD [] +synonym: "methyl violet 10B" BROAD [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0007069 ! crystal violet staining evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007071 +name: flow cell biofilm assay evidence +def: "A type of biofilm formation assay evidence derived from in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3438488/] +is_a: ECO:0006020 ! biofilm formation assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007072 +name: flow cell biofilm assay evidence used in manual assertion +def: "A type of flow cell biofilm assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006021 ! biofilm formation assay evidence used in manual assertion +is_a: ECO:0007071 ! flow cell biofilm assay evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007073 +name: bacterial 2-hybrid assay evidence +def: "A type of bait-prey hybrid interaction evidence that involves the detection of protein-protein interaction by fusing the protein target (the \"bait\") to RNA polymerase and fusing protein or peptide library to be analyzed (the \"prey\") to DNA-binding domain." [URL:http\://www.pnas.org/content/97/13/7382.full.pdf] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007074 +name: bacterial 2-hybrid assay evidence used in manual assertion +def: "A type of bacterial 2-hybrid assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006077 ! bait-prey hybrid interaction evidence used in manual assertion +is_a: ECO:0007073 ! bacterial 2-hybrid assay evidence +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007075 +name: phenomic profiling assay evidence +def: "A type of experimental phenotypic evidence that results from the comparison of phenotype profiles (the physical and biochemical traits of organisms in particular environments) across multiple strains; that is, a meta-analysis of a set of phenotypic patterns from many tests in response to a series of changing genetic and/or environmental factors." [EDAM:topic\:3298, PMID:2118507] +comment: A phenome is defined as the SET of physical and biochemical traits, or, as the SET of all phenotypes expressed, i.e. a phenome is defined as the sum total of all phenotypic traits. +comment: D.S. notes: I think this term is meant to be used for high throughput experiments where a large number of mutants is screened for their ability to grow in a large number of conditions. The individual mutants can then clustered based on the similarity of their phenotypic profiles-whether they grew or didn't grow across all the conditions. The goal being to try to identify genes whose products function in the same biological pathways based on their having similar phenotypes across multiple conditions. +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007076 +name: phenomic profiling assay evidence used in manual assertion +def: "A type of phenomic profiling assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0007075 ! phenomic profiling assay evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007077 +name: colony morphology phenotypic evidence +def: "A type of experimental phenotypic evidence resulting from studying the physical characteristics of an assemblage of microorganisms growing on a solid surface such as the surface of an agar culture medium." [OMP:0000100] +synonym: "colony morphology evidence" EXACT [] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007078 +name: colony morphology phenotypic evidence used in manual assertion +def: "A type of colony morphology phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "colony morphology evidence used in manual assertion" EXACT [] +is_a: ECO:0007077 ! colony morphology phenotypic evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007079 +name: colony color phenotypic evidence +def: "A type of colony morphology evidence resulting from studying the colony pigmentation." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671912/] +synonym: "colony color evidence" EXACT [] +synonym: "colony pigmentation" EXACT [] +is_a: ECO:0007077 ! colony morphology phenotypic evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007080 +name: colony color phenotypic evidence used in manual assertion +def: "A type of colony color phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "colony color evidence used in manual assertion" EXACT [] +synonym: "colony pigmentation" BROAD [] +is_a: ECO:0007078 ! colony morphology phenotypic evidence used in manual assertion +is_a: ECO:0007079 ! colony color phenotypic evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007081 +name: colony size phenotypic evidence +def: "A type of colony morphology phenotypic evidence resulting from measuring the area or diameter of the colony." [URL:http\://www.nature.com/nrmicro/journal/v4/n4/full/nrmicro1384.html] +synonym: "colony size evidence" EXACT [] +is_a: ECO:0007077 ! colony morphology phenotypic evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007082 +name: colony size phenotypic evidence used in manual assertion +def: "A type of colony size phenotypic evidence that is used in an manual assertion." [ECO:RCT] +synonym: "colony area phenotype evidence" NARROW [] +synonym: "colony diameter phenotype evidence" NARROW [] +synonym: "colony size evidence used in manual assertion" EXACT [] +is_a: ECO:0007078 ! colony morphology phenotypic evidence used in manual assertion +is_a: ECO:0007081 ! colony size phenotypic evidence +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007083 +name: zone of inhibition evidence +def: "A type of cell growth assay evidence resulting from assessing the sensitivity or resistance of bacteria or fungi to an antimicrobial agent or chemical by measuring the size of the zone of inhibition that results when the test organism is grown on a solid surface in the presence of the antimicrobial agent or chemical." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC153338/] +is_a: ECO:0001563 ! cell growth assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007084 +name: zone of inhibition evidence used in manual assertion +def: "A type of zone of inhibition evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0007083 ! zone of inhibition evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007085 +name: Etest evidence +def: "A type of zone of inhibition evidence resulting from testing the antimicrobial susceptibility by using predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip." [URL:http\://www.joponline.org/doi/pdf/10.1902/jop.1992.63.7.576] +synonym: "epsilometer test" EXACT [] +is_a: ECO:0007083 ! zone of inhibition evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007086 +name: Etest evidence used in manual assertion +def: "A type of Etest evidence that is used in a manual assertion." [ECO:RCT] +synonym: "epsilometer test" BROAD [] +is_a: ECO:0007084 ! zone of inhibition evidence used in manual assertion +is_a: ECO:0007085 ! Etest evidence +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007087 +name: ribosome profiling evidence +def: "A type of expression pattern evidence in which translation is measured by the positions of ribosomes active in a cell, identified through deep sequencing of ribosome-protected mRNA fragments." [PMID:27015305] +synonym: "ribo-seq evidence" EXACT [] +synonym: "ribosome footprinting evidence" EXACT [] +synonym: "translation profiling" NARROW [] +synonym: "translatome profiling" NARROW [] +is_a: ECO:0000008 ! expression pattern evidence +property_value: IAO:0000234 GOC:VW xsd:string +created_by: rctauber +creation_date: 2018-03-28T10:27:09Z + +[Term] +id: ECO:0007088 +name: ribosome profiling evidence used in manual assertion +def: "A type of ribosome profiling evidence that is used in a manual assertion." [ECO:RCT] +synonym: "ribo-seq evidence" BROAD [] +synonym: "ribosome footprinting evidence" BROAD [] +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +is_a: ECO:0007087 ! ribosome profiling evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2018-03-28T10:33:26Z + +[Term] +id: ECO:0007089 +name: loss-of-function mutant phenotype evidence used in manual assertion +def: "A type of loss-of-function mutant phenotype evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000016 ! loss-of-function mutant phenotype evidence +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007090 +name: structural similarity evidence used in manual assertion +def: "A type of structural similarity evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000027 ! structural similarity evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007092 +name: gain-of-function mutant phenotypic evidence used in manual assertion +def: "A type of gain-of-function mutant phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "gain-of-function mutant phenotype evidence used in manual assertion" EXACT [] +is_a: ECO:0000042 ! gain-of-function mutant phenotypic evidence +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007093 +name: voucher specimen phenotypic analysis evidence used in manual assertion +def: "A type of voucher specimen phenotypic analysis evidence that is used in a manual assertion." [ECO:RCT] +synonym: "voucher specimen analysis evidence used in manual assertion" EXACT [] +is_a: ECO:0000050 ! voucher specimen phenotypic analysis evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007094 +name: positional similarity evidence used in manual assertion +def: "A type of positional similarity evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000060 ! positional similarity evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007095 +name: quantitative trait analysis evidence used in manual assertion +def: "A type of quantitative trait analysis evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000061 ! quantitative trait analysis evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007096 +name: compositional similarity evidence used in manual assertion +def: "A type of compositional similarity evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000063 ! compositional similarity evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007097 +name: developmental similarity evidence used in manual assertion +def: "A type of developmental similarity evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000067 ! developmental similarity evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007098 +name: morphological similarity evidence used in manual assertion +def: "A type of morphological similarity evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000071 ! morphological similarity evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007099 +name: gene expression similarity evidence used in manual assertion +def: "A type of gene expression similarity evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000075 ! gene expression similarity evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007100 +name: methylation-specific polymerase chain reaction evidence used in manual assertion +def: "A type of methylation-specific polymerase chain reaction evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000077 ! methylation-specific polymerase chain reaction evidence +is_a: ECO:0001198 ! in vitro methylation assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007101 +name: southern hybridization evidence used in manual assertion +def: "A type of southern hybridization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000078 ! southern hybridization evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007102 +name: intermethylated site amplification evidence used in manual assertion +def: "A type of intermethylated site amplification evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000086 ! amplification of intermethylated sites evidence +is_a: ECO:0001198 ! in vitro methylation assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007103 +name: epitope-tagged protein immunolocalization evidence used in manual assertion +def: "A type of epitope-tagged protein immunolocalization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000092 ! epitope-tagged protein immunolocalization evidence +is_a: ECO:0006031 ! immunolocalization evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007104 +name: co-fractionation evidence used in manual assertion +def: "A type of co-fractionation evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000102 ! co-fractionation evidence +is_a: ECO:0006005 ! fractionation evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007106 +name: green fluorescent protein fusion protein localization evidence used in manual assertion +def: "A type of green fluorescent protein fusion protein localization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000126 ! green fluorescent protein fusion protein localization evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007107 +name: yellow fluorescent protein fusion protein localization evidence used in manual assertion +def: "A type of yellow fluorescent protein fusion protein localization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000128 ! yellow fluorescent protein fusion protein localization evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007108 +name: beta-glucuronidase fusion protein localization evidence used in manual assertion +def: "A type of beta-glucuronidase fusion protein localization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000130 ! beta-glucuronidase fusion protein localization evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007109 +name: beta-galactosidase fusion protein localization evidence used in manual assertion +def: "A type of beta-galactosidase fusion protein localization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000132 ! beta-galactosidase fusion protein localization evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007110 +name: thin layer chromatography evidence used in manual assertion +def: "A type of thin layer chromatography evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000140 ! thin layer chromatography evidence +is_a: ECO:0007680 ! chromatography evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007111 +name: in vitro recombinant protein transcription reconstitution assay evidence used in manual assertion +def: "A type of in vitro recombinant protein transcription reconstitution assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000152 ! in vitro recombinant protein transcription reconstitution assay evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007112 +name: protein separation followed by direct sequencing evidence used in manual assertion +def: "A type of protein separation followed by direct sequencing evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000158 ! protein separation followed by direct sequencing evidence +is_a: ECO:0006005 ! fractionation evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007113 +name: protein separation followed by fragment identification evidence used in manual assertion +def: "A type of protein separation followed by fragment identification evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000160 ! protein separation followed by fragment identification evidence +is_a: ECO:0006005 ! fractionation evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007114 +name: heterologous system uptake evidence used in manual assertion +def: "A type of heterologous system uptake evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000162 ! heterologous system uptake evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007115 +name: two-electrode voltage clamp recording evidence used in manual assertion +def: "A type of two-electrode voltage clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000166 ! two-electrode voltage clamp recording evidence +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007116 +name: biochemical trait analysis evidence used in manual assertion +def: "A type of biochemical trait analysis evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000172 ! biochemical trait analysis evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007117 +name: mutant physiological response evidence used in manual assertion +def: "A type of mutant physiological response evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000174 ! mutant physiological response evidence +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007118 +name: mutant visible phenotype evidence used in manual assertion +def: "A type of mutant visible phenotype evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000176 ! mutant visible phenotype evidence +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007119 +name: in vivo assay evidence used in manual assertion +def: "A type of in vivo assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000178 ! in vivo assay evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007120 +name: animal model system study evidence used in manual assertion +def: "A type of animal model system study evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000179 ! animal model system study evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007121 +name: clinical study evidence used in manual assertion +def: "A type of clinical study evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000180 ! clinical study evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007122 +name: in vitro assay evidence used in manual assertion +def: "A type of in vitro assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000181 ! in vitro assay evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007123 +name: enzyme inhibition evidence used in manual assertion +def: "A type of enzyme inhibition evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000184 ! enzyme inhibition evidence +is_a: ECO:0005801 ! enzymatic activity assay evidence used in manual assertion +is_a: ECO:0007707 ! protein inhibition evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007125 +name: Illumina sequencing evidence used in manual assertion +def: "A type of Illumina sequencing evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000222 ! Illumina sequencing evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007126 +name: 454 pyrosequencing evidence used in manual assertion +def: "A type of 454 pyrosequencing evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000223 ! 454 pyrosequencing evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007127 +name: SOLiD sequencing evidence used in manual assertion +def: "A type of SOLiD sequencing evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000224 ! SOLiD sequencing evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007128 +name: chain termination sequencing evidence used in manual assertion +def: "A type of chain termination sequencing evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000225 ! chain termination sequencing evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007129 +name: chromatin immunoprecipitation-qPCR evidence used in manual assertion +def: "A type of chromatin immunoprecipitation-qPCR evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000228 ! chromatin immunoprecipitation-qPCR evidence +is_a: ECO:0005644 ! immunoprecipitation evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007130 +name: 4C evidence used in manual assertion +def: "A type of 4C evidence that is used in a manual assertion." [ECO:RCT] +synonym: "circularized chromosome conformation capture evidence used in manual assertion" EXACT [] +is_a: ECO:0000234 ! 4C evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007131 +name: 5C evidence used in manual assertion +def: "A type of 5C evidence that is used in a manual assertion." [ECO:RCT] +synonym: "carbon-copy chromosome conformation capture evidence used in manual assertion" EXACT [] +is_a: ECO:0000235 ! 5C evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007133 +name: 3C-qPCR evidence used in manual assertion +def: "A type of 3C-qPCR evidence that is used in a manual assertion." [ECO:RCT] +synonym: "chromosome conformation capture-qPCR evidence used in manual assertion" EXACT [] +is_a: ECO:0000237 ! 3C-qPCR evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007134 +name: Hi-C evidence used in manual assertion +def: "A type of Hi-C evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000238 ! Hi-C evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007135 +name: 3C-seq evidence used in manual assertion +def: "A type of 3C-seq evidence that is used in a manual assertion." [ECO:RCT] +synonym: "chromosome conformation capture sequencing evidence used in manual assertion" EXACT [] +is_a: ECO:0000239 ! 3C-seq evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007136 +name: environmental perturbation phenotypic evidence used in manual assertion +def: "A type of environmental perturbation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "environmental perturbation evidence used in manual assertion" EXACT [] +is_a: ECO:0000241 ! environmental perturbation phenotypic evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007137 +name: tissue ablation phenotypic evidence used in manual assertion +def: "A type of tissue ablation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "tissue ablation evidence used in manual assertion" EXACT [] +is_a: ECO:0000242 ! tissue ablation phenotypic evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007138 +name: tissue grafting phenotypic evidence used in manual assertion +def: "A type of tissue grafting phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "tissue grafting evidence used in manual assertion" EXACT [] +is_a: ECO:0000243 ! tissue grafting phenotypic evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007141 +name: cytochalasin experiment evidence used in manual assertion +def: "A type of cytochalasin experiment evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000299 ! cytochalasin experiment evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007142 +name: green fluorescent protein immunolocalization evidence used in manual assertion +def: "A type of green fluorescent protein immunolocalization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000300 ! green fluorescent protein immunolocalization evidence +is_a: ECO:0006031 ! immunolocalization evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007143 +name: beta-galactosidase protein immunolocalization evidence used in manual assertion +def: "A type of beta-galactosidase protein immunolocalization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000301 ! beta-galactosidase protein immunolocalization evidence +is_a: ECO:0006031 ! immunolocalization evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007144 +name: cap analysis of gene expression evidence used in manual assertion +def: "A type of cap analysis of gene expression evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000291 ! transcript expression evidence used in manual assertion +is_a: ECO:0000309 ! cap analysis of gene expression evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007145 +name: nano-cap analysis of gene expression evidence used in manual assertion +def: "A type of nano-cap analysis of gene expression evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000291 ! transcript expression evidence used in manual assertion +is_a: ECO:0000310 ! nano-cap analysis of gene expression evidence +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007146 +name: particle size and count assay evidence used in manual assertion +def: "A type of particle size and count assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0000334 ! particle size and count assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007147 +name: competitive growth assay evidence used in manual assertion +def: "A type of competitive growth assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0000336 ! competitive growth assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007148 +name: pulsed-field gel electrophoresis evidence used in manual assertion +def: "A type of pulsed-field gel electrophoresis evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0000338 ! pulsed-field gel electrophoresis evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007149 +name: two-dimensional agarose gel electrophoresis evidence used in manual assertion +def: "A type of two-dimensional agarose gel electrophoresis evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0000339 ! two-dimensional agarose gel electrophoresis evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007150 +name: plasmid maintenance assay evidence used in manual assertion +def: "A type of plasmid maintenance assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0000340 ! plasmid maintenance assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007151 +name: specific protein inhibition by antibody evidence used in manual assertion +def: "A type of specific protein inhibition by antibody evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000341 ! specific protein inhibition by antibody evidence +is_a: ECO:0007707 ! protein inhibition evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007152 +name: single exon transcript confirmation via alignment evidence used in manual assertion +def: "A type of single exon transcript confirmation via alignment evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000247 ! sequence alignment evidence used in manual assertion +is_a: ECO:0000345 ! single exon transcript confirmation via alignment evidence +property_value: ECO:9000002 "ISA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007153 +name: phylogenetic distribution evidence used in manual assertion +def: "A type of phylogenetic distribution evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000252 ! similarity evidence used in manual assertion +is_a: ECO:0000355 ! phylogenetic distribution evidence +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007154 +name: differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) used in manual assertion +def: "A type of differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000291 ! transcript expression evidence used in manual assertion +is_a: ECO:0000358 ! differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007155 +name: differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) used in manual assertion +def: "A type of differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000291 ! transcript expression evidence used in manual assertion +is_a: ECO:0000359 ! differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007156 +name: biological target-disease association via drug evidence used in manual assertion +def: "A type of biological target-disease association via drug that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0000360 ! biological target-disease association via drug evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007157 +name: cell staining evidence used in manual assertion +def: "A type of cell staining evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001603 ! cell staining evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007158 +name: visual sequence inspection evidence used in manual assertion +def: "A type of visual sequence inspection evidence that is used in a manual assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007159 +name: ATP bioluminescence assay evidence used in manual assertion +def: "A type of ATP bioluminescence assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005011 ! ATP bioluminescence assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007160 +name: missense mutation pohenotypic evidence used in manual assertion +def: "A type of missense mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "missense mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0001247 ! point mutation phenotypic evidence used in manual assertion +is_a: ECO:0005506 ! missense mutation phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007161 +name: nonsense mutation phenotypic evidence used in manual assertion +def: "A type of nonsense mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "nonsense mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0001247 ! point mutation phenotypic evidence used in manual assertion +is_a: ECO:0005507 ! nonsense mutation phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007162 +name: silent mutation evidence used in manual assertion +def: "A type of silent mutation evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001247 ! point mutation phenotypic evidence used in manual assertion +is_a: ECO:0005508 ! silent mutation evidence +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007163 +name: insertion mutation phenotypic evidence used in manual assertion +def: "A type of insertion mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "insertion mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005509 ! insertion mutation phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007164 +name: duplication mutation evidence used in manual assertion +def: "A type of duplication mutation evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005511 ! duplication mutation evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007165 +name: frameshift mutation phenotypic evidence used in manual assertion +def: "A type of frameshift mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "frameshift mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005512 ! frameshift mutation phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007166 +name: repeat expansion mutation phenotypic evidence used in manual assertion +def: "A type of repeat expansion mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "repeat expansion mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005513 ! repeat expansion mutation phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007167 +name: splice site mutation phenotypic evidence used in manual assertion +def: "A type of splice site mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "splice site mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005514 ! splice site mutation phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007168 +name: translocation mutation phenotypic evidence used in manual assertion +def: "A type of translocation mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "translocation mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005515 ! translocation mutation phenotypic evidence +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007169 +name: in-gel protein kinase assay evidence used in manual assertion +def: "A type of in-gel protein kinase assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005562 ! in-gel protein kinase assay evidence +is_a: ECO:0005801 ! enzymatic activity assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007170 +name: macroscopic current trace evidence used in manual assertion +def: "A type of macroscopic current trace evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005563 ! macroscopic current trace evidence +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007171 +name: current density evidence used in manual assertion +def: "A type of current density evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005564 ! current density evidence +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007172 +name: sustained current evidence used in manual assertion +def: "A type of sustained current evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005566 ! sustained current evidence +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007173 +name: use dependence of inactivation evidence used in manual assertion +def: "A type of use dependence of inactivation evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005570 ! use dependence of inactivation evidence +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007174 +name: current clamp recording evidence used in manual assertion +def: "A type of current clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005571 ! current clamp recording evidence +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007175 +name: whole-cell voltage clamp recording evidence used in manual assertion +def: "A type of whole-cell voltage clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005572 ! whole-cell voltage clamp recording evidence +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007176 +name: cell-attached single-channel recording evidence used in manual assertion +def: "A type of cell-attached single-channel recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005573 ! cell-attached single-channel recording evidence +is_a: ECO:0006013 ! patch-clamp recording evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007177 +name: cell-detached inside-out single-channel recording evidence used in manual assertion +def: "A type of cell-detached inside-out single-channel recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005574 ! cell-detached inside-out single-channel recording evidence +is_a: ECO:0006013 ! patch-clamp recording evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007178 +name: reconstituted bilayer single-channel patch recording evidence used in manual assertion +def: "A type of reconstituted bilayer single-channel patch recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005575 ! reconstituted bilayer single-channel patch recording evidence +is_a: ECO:0006013 ! patch-clamp recording evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007179 +name: electroencephalography recording evidence used in manual assertion +def: "A type of electroencephalography recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005577 ! electroencephalography recording evidence +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007180 +name: cell-detached outside-out single-channel recording evidence used in manual assertion +def: "A type of cell-detached outside-out single-channel recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005582 ! cell-detached outside-out single-channel recording evidence +is_a: ECO:0006013 ! patch-clamp recording evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007181 +name: cut-open oocyte voltage clamp recording evidence used in manual assertion +def: "A type of cut-open oocyte voltage clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005583 ! cut-open oocyte voltage clamp recording evidence +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007182 +name: macropatch voltage clamp recording evidence used in manual assertion +def: "A type of macropatch voltage clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005584 ! macropatch voltage clamp recording evidence +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007184 +name: protein mass spectrometry evidence used in manual assertion +def: "A type of protein mass spectrometry evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001230 ! mass spectrometry evidence used in manual assertion +is_a: ECO:0005603 ! protein mass spectrometry evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007185 +name: cross-streak test evidence used in manual assertion +def: "A type of cross-streak test evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005604 ! cross-streak test evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007186 +name: tethered cell assay evidence used in manual assertion +def: "A type of tethered cell assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005607 ! tethered cell assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007187 +name: tumble frequency assay evidence used in manual assertion +def: "A type of tumble frequency assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005608 ! tumble frequency assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007188 +name: capillary assay evidence used in manual assertion +def: "A type of capillary assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005609 ! capillary assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007189 +name: inference from experimental data evidence used in manual assertion +def: "A type of inference from experimental data evidence that is used in a manual assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007190 +name: inference from phenotype manipulation evidence used in manual assertion +def: "A type of inference from phenotype manipulation evidence that is used in a manual assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007191 +name: inference by association of genotype from phenotype used in manual assertion +def: "A type of inference by association of genotype from phenotype that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001237 ! natural variation mutant evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007192 +name: motility assay evidence used in manual assertion +def: "A type of motility assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005803 ! motility assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007193 +name: loss-of-function mutant phenotype evidence used in automatic assertion +def: "A type of loss-of-function mutant phenotype evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000016 ! loss-of-function mutant phenotype evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007194 +name: structural similarity evidence used in automatic assertion +def: "A type of structural similarity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000027 ! structural similarity evidence +is_a: ECO:0007824 ! phenotypic similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007196 +name: gain-of-function mutant phenotypic evidence used in automatic assertion +def: "A type of gain-of-function mutant phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "gain-of-function mutant phenotype evidence used in automatic assertion" EXACT [] +is_a: ECO:0000042 ! gain-of-function mutant phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007197 +name: voucher specimen phenotypic analysis evidence used in automatic assertion +def: "A type of voucher specimen phenotypic analysis evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "voucher specimen analysis evidence used in automatic assertion" EXACT [] +is_a: ECO:0000050 ! voucher specimen phenotypic analysis evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007198 +name: positional similarity evidence used in automatic assertion +def: "A type of positional similarity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000060 ! positional similarity evidence +is_a: ECO:0007824 ! phenotypic similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007199 +name: quantitative trait analysis evidence used in automatic assertion +def: "A type of quantitative trait analysis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000061 ! quantitative trait analysis evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007200 +name: compositional similarity evidence used in automatic assertion +def: "A type of compositional similarity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000063 ! compositional similarity evidence +is_a: ECO:0007824 ! phenotypic similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007201 +name: developmental similarity evidence used in automatic assertion +def: "A type of developmental similarity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000067 ! developmental similarity evidence +is_a: ECO:0007824 ! phenotypic similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007202 +name: morphological similarity evidence used in automatic assertion +def: "A type of morphological similarity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000071 ! morphological similarity evidence +is_a: ECO:0007824 ! phenotypic similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007203 +name: gene expression similarity evidence used in automatic assertion +def: "A type of gene expression similarity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000075 ! gene expression similarity evidence +is_a: ECO:0007824 ! phenotypic similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007204 +name: methylation-specific polymerase chain reaction evidence used in automatic assertion +def: "A type of methylation-specific polymerase chain reaction evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000077 ! methylation-specific polymerase chain reaction evidence +is_a: ECO:0007401 ! in vitro methylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007205 +name: southern hybridization evidence used in automatic assertion +def: "A type of southern hybridization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000078 ! southern hybridization evidence +is_a: ECO:0007817 ! DNA detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007206 +name: intermethylated site amplification evidence used in automatic assertion +def: "A type of intermethylated site amplification evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000086 ! amplification of intermethylated sites evidence +is_a: ECO:0007401 ! in vitro methylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007207 +name: epitope-tagged protein immunolocalization evidence used in automatic assertion +def: "A type of epitope-tagged protein immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000092 ! epitope-tagged protein immunolocalization evidence +is_a: ECO:0007486 ! immunolocalization evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007208 +name: co-fractionation evidence used in automatic assertion +def: "A type of co-fractionation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000102 ! co-fractionation evidence +is_a: ECO:0007534 ! fractionation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007210 +name: green fluorescent protein fusion protein localization evidence used in automatic assertion +def: "A type of green fluorescent protein fusion protein localization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000126 ! green fluorescent protein fusion protein localization evidence +is_a: ECO:0007808 ! fusion protein localization evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007211 +name: yellow fluorescent protein fusion protein localization evidence used in automatic assertion +def: "A type of yellow fluorescent protein fusion protein localization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000128 ! yellow fluorescent protein fusion protein localization evidence +is_a: ECO:0007808 ! fusion protein localization evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007212 +name: beta-glucuronidase fusion protein localization evidence used in automatic assertion +def: "A type of beta-glucuronidase fusion protein localization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000130 ! beta-glucuronidase fusion protein localization evidence +is_a: ECO:0007808 ! fusion protein localization evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007213 +name: beta-galactosidase fusion protein localization evidence used in automatic assertion +def: "A type of beta-galactosidase fusion protein localization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000132 ! beta-galactosidase fusion protein localization evidence +is_a: ECO:0007808 ! fusion protein localization evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007214 +name: thin layer chromatography evidence used in automatic assertion +def: "A type of thin layer chromatography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000140 ! thin layer chromatography evidence +is_a: ECO:0007681 ! chromatography evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007215 +name: in vitro recombinant protein transcription reconstitution assay evidence used in automatic assertion +def: "A type of in vitro recombinant protein transcription reconstitution assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000152 ! in vitro recombinant protein transcription reconstitution assay evidence +is_a: ECO:0007804 ! in vitro transcription reconstitution assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007216 +name: protein separation followed by direct sequencing evidence used in automatic assertion +def: "A type of protein separation followed by direct sequencing evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000158 ! protein separation followed by direct sequencing evidence +is_a: ECO:0007787 ! protein separation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007217 +name: protein separation followed by fragment identification evidence used in automatic assertion +def: "A type of protein separation followed by fragment identification evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000160 ! protein separation followed by fragment identification evidence +is_a: ECO:0007787 ! protein separation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007218 +name: heterologous system uptake evidence used in automatic assertion +def: "A type of heterologous system uptake evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000162 ! heterologous system uptake evidence +is_a: ECO:0007789 ! transport assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007219 +name: two-electrode voltage clamp recording evidence used in automatic assertion +def: "A type of two-electrode voltage clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000166 ! two-electrode voltage clamp recording evidence +is_a: ECO:0007766 ! voltage clamp recording evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007220 +name: biochemical trait analysis evidence used in automatic assertion +def: "A type of biochemical trait analysis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000172 ! biochemical trait analysis evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007221 +name: mutant physiological response evidence used in automatic assertion +def: "A type of mutant physiological response evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000174 ! mutant physiological response evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007222 +name: mutant visible phenotype evidence used in automatic assertion +def: "A type of mutant visible phenotype evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000176 ! mutant visible phenotype evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007223 +name: in vivo assay evidence used in automatic assertion +def: "A type of in vivo assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000178 ! in vivo assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007224 +name: animal model system study evidence used in automatic assertion +def: "A type of animal model system study evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000179 ! animal model system study evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007225 +name: clinical study evidence used in automatic assertion +def: "A type of clinical study evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000180 ! clinical study evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007226 +name: in vitro assay evidence used in automatic assertion +def: "A type of in vitro assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000181 ! in vitro assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007227 +name: enzyme inhibition evidence used in automatic assertion +def: "A type of enzyme inhibition evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000184 ! enzyme inhibition evidence +is_a: ECO:0007468 ! enzymatic activity assay evidence used in automatic assertion +is_a: ECO:0007785 ! protein inhibition evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007229 +name: Illumina sequencing evidence used in automatic assertion +def: "A type of Illumina sequencing evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000222 ! Illumina sequencing evidence +is_a: ECO:0007814 ! high throughput nucleotide sequencing assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007230 +name: 454 pyrosequencing evidence used in automatic assertion +def: "A type of 454 pyrosequencing evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000223 ! 454 pyrosequencing evidence +is_a: ECO:0007814 ! high throughput nucleotide sequencing assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007231 +name: SOLiD sequencing evidence used in automatic assertion +def: "A type of SOLiD sequencing evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000224 ! SOLiD sequencing evidence +is_a: ECO:0007814 ! high throughput nucleotide sequencing assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007232 +name: chain termination sequencing evidence used in automatic assertion +def: "A type of chain termination sequencing evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000225 ! chain termination sequencing evidence +is_a: ECO:0007813 ! nucleotide sequencing assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007233 +name: chromatin immunoprecipitation-qPCR evidence used in automatic assertion +def: "A type of chromatin immunoprecipitation-qPCR evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000228 ! chromatin immunoprecipitation-qPCR evidence +is_a: ECO:0007786 ! chromatin immunoprecipitation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007234 +name: 4C evidence used in automatic assertion +def: "A type of 4C evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "circularized chromosome conformation capture evidence used in automatic assertion" EXACT [] +is_a: ECO:0000234 ! 4C evidence +is_a: ECO:0007822 ! chromosome conformation-based evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007235 +name: 5C evidence used in automatic assertion +def: "A type of 5C evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "carbon-copy chromosome conformation capture evidence used in automatic assertion" EXACT [] +is_a: ECO:0000235 ! 5C evidence +is_a: ECO:0007822 ! chromosome conformation-based evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007237 +name: 3C-qPCR evidence used in automatic assertion +def: "A type of 3C-qPCR evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "chromosome conformation capture-qPCR evidence used in automatic assertion" EXACT [] +is_a: ECO:0000237 ! 3C-qPCR evidence +is_a: ECO:0007823 ! 3C evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007238 +name: Hi-C evidence used in automatic assertion +def: "A type of Hi-C evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000238 ! Hi-C evidence +is_a: ECO:0007822 ! chromosome conformation-based evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007239 +name: 3C-seq evidence used in automatic assertion +def: "A type of 3C-seq evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "chromosome conformation capture sequencing evidence used in automatic assertion" EXACT [] +is_a: ECO:0000239 ! 3C-seq evidence +is_a: ECO:0007822 ! chromosome conformation-based evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007240 +name: environmental perturbation phenotypic evidence used in automatic assertion +def: "A type of environmental perturbation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "environmental perturbation evidence used in automatic assertion" EXACT [] +is_a: ECO:0000241 ! environmental perturbation phenotypic evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007241 +name: tissue ablation phenotypic evidence used in automatic assertion +def: "A type of tissue ablation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "tissue ablation evidence used in automatic assertion" EXACT [] +is_a: ECO:0000242 ! tissue ablation phenotypic evidence +is_a: ECO:0007810 ! anatomical perturbation phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007242 +name: tissue grafting phenotypic evidence used in automatic assertion +def: "A type of tissue grafting phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "tissue grafting evidence used in automatic assertion" EXACT [] +is_a: ECO:0000243 ! tissue grafting phenotypic evidence +is_a: ECO:0007810 ! anatomical perturbation phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007245 +name: cytochalasin experiment evidence used in automatic assertion +def: "A type of cytochalasin experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000299 ! cytochalasin experiment evidence +is_a: ECO:0007811 ! cleavage arrested development evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007246 +name: green fluorescent protein immunolocalization evidence used in automatic assertion +def: "A type of green fluorescent protein immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000300 ! green fluorescent protein immunolocalization evidence +is_a: ECO:0007486 ! immunolocalization evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007247 +name: beta-galactosidase protein immunolocalization evidence used in automatic assertion +def: "A type of beta-galactosidase protein immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000301 ! beta-galactosidase protein immunolocalization evidence +is_a: ECO:0007486 ! immunolocalization evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007248 +name: cap analysis of gene expression evidence used in automatic assertion +def: "A type of cap analysis of gene expression evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000309 ! cap analysis of gene expression evidence +is_a: ECO:0007303 ! transcript expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007249 +name: nano-cap analysis of gene expression evidence used in automatic assertion +def: "A type of nano-cap analysis of gene expression evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000310 ! nano-cap analysis of gene expression evidence +is_a: ECO:0007303 ! transcript expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007250 +name: particle size and count assay evidence used in automatic assertion +def: "A type of particle size and count assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000334 ! particle size and count assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007251 +name: competitive growth assay evidence used in automatic assertion +def: "A type of competitive growth assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000336 ! competitive growth assay evidence +is_a: ECO:0007775 ! cell proliferation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007252 +name: pulsed-field gel electrophoresis evidence used in automatic assertion +def: "A type of pulsed-field gel electrophoresis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000338 ! pulsed-field gel electrophoresis evidence +is_a: ECO:0007769 ! gel electrophoresis evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007253 +name: two-dimensional agarose gel electrophoresis evidence used in automatic assertion +def: "A type of two-dimensional agarose gel electrophoresis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000339 ! two-dimensional agarose gel electrophoresis evidence +is_a: ECO:0007769 ! gel electrophoresis evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007254 +name: plasmid maintenance assay evidence used in automatic assertion +def: "A type of plasmid maintenance assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000340 ! plasmid maintenance assay evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007255 +name: specific protein inhibition by antibody evidence used in automatic assertion +def: "A type of specific protein inhibition by antibody evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000341 ! specific protein inhibition by antibody evidence +is_a: ECO:0007785 ! protein inhibition evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007256 +name: single exon transcript confirmation via alignment evidence used in automatic assertion +def: "A type of single exon transcript confirmation via alignment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000248 ! sequence alignment evidence used in automatic assertion +is_a: ECO:0000345 ! single exon transcript confirmation via alignment evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007257 +name: phylogenetic distribution evidence used in automatic assertion +def: "A type of phylogenetic distribution evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000355 ! phylogenetic distribution evidence +is_a: ECO:0007826 ! phylogenetic evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007258 +name: differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) used in automatic assertion +def: "A type of differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000358 ! differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) +is_a: ECO:0007303 ! transcript expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007259 +name: differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) used in automatic assertion +def: "A type of differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000359 ! differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) +is_a: ECO:0007303 ! transcript expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007260 +name: biological target-disease association via drug evidence used in automatic assertion +def: "A type of biological target-disease association via drug that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000360 ! biological target-disease association via drug evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007261 +name: cell staining evidence used in automatic assertion +def: "A type of cell staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001603 ! cell staining evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007262 +name: visual sequence inspection evidence used in automatic assertion +def: "A type of visual sequence inspection evidence that is used in an automatic assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007263 +name: ATP bioluminescence assay evidence used in automatic assertion +def: "A type of ATP bioluminescence assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005011 ! ATP bioluminescence assay evidence +is_a: ECO:0007774 ! cell viability assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007264 +name: missense mutation phenotypic evidence used in automatic assertion +def: "A type of missense mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "missense mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005506 ! missense mutation phenotypic evidence +is_a: ECO:0007448 ! point mutation phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007265 +name: nonsense mutation phenotypic evidence used in automatic assertion +def: "A type of nonsense mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "nonsense mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005507 ! nonsense mutation phenotypic evidence +is_a: ECO:0007448 ! point mutation phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007266 +name: silent mutation evidence used in automatic assertion +def: "A type of silent mutation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005508 ! silent mutation evidence +is_a: ECO:0007448 ! point mutation phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007267 +name: insertion mutation phenotypic evidence used in automatic assertion +def: "A type of insertion mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "insertion mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005509 ! insertion mutation phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007268 +name: duplication mutation evidence used in automatic assertion +def: "A type of duplication mutation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005511 ! duplication mutation evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007269 +name: frameshift mutation phenotypic evidence used in automatic assertion +def: "A type of frameshift mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "frameshift mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005512 ! frameshift mutation phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007270 +name: repeat expansion mutation phenotypic evidence used in automatic assertion +def: "A type of repeat expansion mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "repeat expansion mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005513 ! repeat expansion mutation phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007271 +name: splice site mutation phenotypic evidence used in automatic assertion +def: "A type of splice site mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "splice site mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005514 ! splice site mutation phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007272 +name: translocation mutation phenotypic evidence used in automatic assertion +def: "A type of translocation mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "translocation mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005515 ! translocation mutation phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007273 +name: in-gel protein kinase assay evidence used in automatic assertion +def: "A type of in-gel protein kinase assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005562 ! in-gel protein kinase assay evidence +is_a: ECO:0007767 ! protein kinase assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007274 +name: macroscopic current trace evidence used in automatic assertion +def: "A type of macroscopic current trace evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005563 ! macroscopic current trace evidence +is_a: ECO:0007766 ! voltage clamp recording evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007275 +name: current density evidence used in automatic assertion +def: "A type of current density evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005564 ! current density evidence +is_a: ECO:0007535 ! electrophysiology assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007276 +name: sustained current evidence used in automatic assertion +def: "A type of sustained current evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005566 ! sustained current evidence +is_a: ECO:0007535 ! electrophysiology assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007277 +name: use dependence of inactivation evidence used in automatic assertion +def: "A type of use dependence of inactivation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005570 ! use dependence of inactivation evidence +is_a: ECO:0007535 ! electrophysiology assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007278 +name: current clamp recording evidence used in automatic assertion +def: "A type of current clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005571 ! current clamp recording evidence +is_a: ECO:0007535 ! electrophysiology assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007279 +name: whole-cell voltage clamp recording evidence used in automatic assertion +def: "A type of whole-cell voltage clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005572 ! whole-cell voltage clamp recording evidence +is_a: ECO:0007766 ! voltage clamp recording evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007280 +name: cell-attached single-channel recording evidence used in automatic assertion +def: "A type of cell-attached single-channel recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005573 ! cell-attached single-channel recording evidence +is_a: ECO:0007537 ! patch-clamp recording evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007281 +name: cell-detached inside-out single-channel recording evidence used in automatic assertion +def: "A type of cell-detached inside-out single-channel recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005574 ! cell-detached inside-out single-channel recording evidence +is_a: ECO:0007537 ! patch-clamp recording evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007282 +name: reconstituted bilayer single-channel patch recording evidence used in automatic assertion +def: "A type of reconstituted bilayer single-channel patch recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005575 ! reconstituted bilayer single-channel patch recording evidence +is_a: ECO:0007537 ! patch-clamp recording evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007283 +name: electroencephalography recording evidence used in automatic assertion +def: "A type of electroencephalography recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005577 ! electroencephalography recording evidence +is_a: ECO:0007535 ! electrophysiology assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007284 +name: cell-detached outside-out single-channel recording evidence used in automatic assertion +def: "A type of cell-detached outside-out single-channel recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005582 ! cell-detached outside-out single-channel recording evidence +is_a: ECO:0007537 ! patch-clamp recording evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007285 +name: cut-open oocyte voltage clamp recording evidence used in automatic assertion +def: "A type of cut-open oocyte voltage clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005583 ! cut-open oocyte voltage clamp recording evidence +is_a: ECO:0007766 ! voltage clamp recording evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007286 +name: macropatch voltage clamp recording evidence used in automatic assertion +def: "A type of macropatch voltage clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005584 ! macropatch voltage clamp recording evidence +is_a: ECO:0007766 ! voltage clamp recording evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007288 +name: protein mass spectrometry evidence used in automatic assertion +def: "A type of protein mass spectrometry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005603 ! protein mass spectrometry evidence +is_a: ECO:0007433 ! mass spectrometry evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007289 +name: cross-streak test evidence used in automatic assertion +def: "A type of cross-streak test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005604 ! cross-streak test evidence +is_a: ECO:0007775 ! cell proliferation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007290 +name: tethered cell assay evidence used in automatic assertion +def: "A type of tethered cell assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005607 ! tethered cell assay evidence +is_a: ECO:0007780 ! chemotaxis assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007291 +name: tumble frequency assay evidence used in automatic assertion +def: "A type of tumble frequency assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005608 ! tumble frequency assay evidence +is_a: ECO:0007780 ! chemotaxis assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007292 +name: capillary assay evidence used in automatic assertion +def: "A type of capillary assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005609 ! capillary assay evidence +is_a: ECO:0007780 ! chemotaxis assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007293 +name: inference from experimental data evidence used in automatic assertion +def: "A type of inference from experimental data evidence that is used in an automatic assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007294 +name: inference from phenotype manipulation evidence used in automatic assertion +def: "A type of inference from phenotype manipulation evidence that is used in an automatic assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007295 +name: inference by association of genotype from phenotype used in automatic assertion +def: "A type of inference by association of genotype from phenotype that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005613 ! inference by association of genotype from phenotype +is_a: ECO:0007191 ! inference by association of genotype from phenotype used in manual assertion +is_a: ECO:0007440 ! natural variation mutant evidence used in automatic assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007296 +name: motility assay evidence used in automatic assertion +def: "A type of motility assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005803 ! motility assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007297 +name: experimental evidence used in automatic assertion +def: "A type of experimental evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000006 ! experimental evidence +is_a: ECO:0000501 ! evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007298 +name: expression pattern evidence used in automatic assertion +def: "A type of expression pattern evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000008 ! expression pattern evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007299 +name: Affymetrix GeneChip evidence used in automatic assertion +def: "A type of Affymetrix GeneChip evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000101 ! Affymetrix GeneChip evidence +is_a: ECO:0007300 ! cRNA to DNA expression microarray evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007300 +name: cRNA to DNA expression microarray evidence used in automatic assertion +def: "A type of cRNA to DNA expression microarray evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000062 ! cRNA to DNA expression microarray evidence +is_a: ECO:0007301 ! expression microarray evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007301 +name: expression microarray evidence used in automatic assertion +def: "A type of expression microarray evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000058 ! expression microarray evidence +is_a: ECO:0007303 ! transcript expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007302 +name: differential methylation hybridization evidence used in automatic assertion +def: "A type of differential methylation hybridization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000069 ! differential methylation hybridization evidence +is_a: ECO:0007401 ! in vitro methylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007303 +name: transcript expression evidence used in automatic assertion +def: "A type of transcript expression evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "transcriptomics evidence used in automatic assertion" EXACT [] +is_a: ECO:0000009 ! transcript expression evidence +is_a: ECO:0007298 ! expression pattern evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007304 +name: Nimblegen array evidence used in automatic assertion +def: "A type of Nimblegen array evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000105 ! Nimblegen array evidence +is_a: ECO:0005630 ! cDNA to DNA expression microarray evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007305 +name: array-based sequence capture evidence used in automatic assertion +def: "A type of array-based sequence capture evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000093 ! array-based sequence capture evidence +is_a: ECO:0007817 ! DNA detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007306 +name: qualitative western immunoblotting evidence used in automatic assertion +alt_id: ECO:0007287 +def: "A type of qualitative western immunoblotting evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "protein expression level evidence based on western blot used in automatic assertion" EXACT [] +is_a: ECO:0000112 ! qualitative western immunoblotting evidence +is_a: ECO:0007309 ! protein expression evidence used in automatic assertion +is_a: ECO:0007770 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence used in automatic assertion +is_a: ECO:0007796 ! immunodetection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007307 +name: direct assay evidence used in automatic assertion +def: "A type of direct assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000002 ! direct assay evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007309 +name: protein expression evidence used in automatic assertion +alt_id: ECO:0007308 +def: "A type of protein expression evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "protein expression level evidence used in automatic assertion" EXACT [] +is_a: ECO:0000010 ! protein expression evidence +is_a: ECO:0007298 ! expression pattern evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007310 +name: expression library screen evidence used in automatic assertion +def: "A type of expression library screen evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000114 ! expression library screen evidence +is_a: ECO:0007309 ! protein expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007311 +name: heterologous protein expression evidence used in automatic assertion +def: "A type of heterologous protein expression evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000154 ! heterologous protein expression evidence +is_a: ECO:0007309 ! protein expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007312 +name: spatial pattern of protein expression evidence used in automatic assertion +def: "A type of spatial pattern of protein expression evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000045 ! spatial pattern of protein expression evidence +is_a: ECO:0007309 ! protein expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007313 +name: DNA to cDNA expression microarray evidence used in automatic assertion +def: "A type of DNA to cDNA expression microarray evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000104 ! DNA to cDNA expression microarray evidence +is_a: ECO:0007301 ! expression microarray evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007314 +name: differential hybridization evidence used in automatic assertion +def: "A type of differential hybridization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000116 ! differential hybridization evidence +is_a: ECO:0007303 ! transcript expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007315 +name: RNA protection assay evidence used in automatic assertion +def: "A type of RNA protection assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000110 ! RNA protection assay evidence +is_a: ECO:0007316 ! nuclease protection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007316 +name: nuclease protection assay evidence used in automatic assertion +def: "A type of nuclease protection assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001106 ! nuclease protection assay evidence +is_a: ECO:0007818 ! RNA detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007317 +name: spatial pattern of transcript expression evidence used in automatic assertion +def: "A type of spatial pattern of transcript expression evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000047 ! spatial pattern of transcript expression evidence +is_a: ECO:0007303 ! transcript expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007318 +name: subtractive hybridization evidence used in automatic assertion +def: "A type of subtractive hybridization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000118 ! subtractive hybridization evidence +is_a: ECO:0007303 ! transcript expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007319 +name: author statement used in automatic assertion +def: "A type of author statement that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000204 ! author statement +is_a: ECO:0006156 ! documented statement evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007320 +name: author statement without traceable support used in automatic assertion +def: "A type of author statement without traceable support that is used in an automatic assertion." [ECO:RCT] +synonym: "non-traceable author statement used in automatic assertion" RELATED [] +is_a: ECO:0000034 ! author statement without traceable support +is_a: ECO:0007319 ! author statement used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007321 +name: author statement supported by traceable reference used in automatic assertion +def: "A type of author statement supported by traceable reference that is used in an automatic assertion." [ECO:RCT] +synonym: "traceable author statement used in automatic assertion" RELATED [] +is_a: ECO:0000033 ! author statement supported by traceable reference +is_a: ECO:0007319 ! author statement used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007322 +name: curator inference used in automatic assertion +def: "A type of curator inference that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000205 ! curator inference +is_a: ECO:0007832 ! inferential evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007323 +name: inference from background scientific knowledge used in automatic assertion +def: "A type of inference from background scientific knowledge that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000001 ! inference from background scientific knowledge +is_a: ECO:0007322 ! curator inference used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007324 +name: no evidence data found used in automatic assertion +def: "An inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000035 ! no evidence data found +is_a: ECO:0007322 ! curator inference used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007325 +name: mutant phenotype evidence used in automatic assertion +def: "A type of mutant phenotype evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000015 ! mutant phenotype evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007326 +name: genetic interaction evidence used in automatic assertion +def: "A type of genetic interaction evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000011 ! genetic interaction evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007327 +name: genomic context evidence used in automatic assertion +def: "A type of genomic context evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000177 ! genomic context evidence +is_a: ECO:0000251 ! similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007328 +name: biological aspect of ancestor evidence used in automatic assertion +def: "A type of biological aspect of ancestor evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000308 ! biological aspect of ancestor evidence +is_a: ECO:0007826 ! phylogenetic evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007329 +name: biological aspect of descendant evidence used in automatic assertion +def: "A type of biological aspect of descendant evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000214 ! biological aspect of descendant evidence +is_a: ECO:0007826 ! phylogenetic evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007330 +name: phylogenetic determination of loss of key residues evidence used in automatic assertion +def: "A type of phylogenetic determination of loss of key residues evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000216 ! phylogenetic determination of loss of key residues evidence +is_a: ECO:0007826 ! phylogenetic evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007331 +name: rapid divergence from ancestral sequence evidence used in automatic assertion +def: "A type of rapid divergence from ancestral sequence evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000215 ! rapid divergence from ancestral sequence evidence +is_a: ECO:0007826 ! phylogenetic evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007332 +name: physical interaction evidence used in automatic assertion +def: "A type of physical interaction evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000021 ! physical interaction evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007333 +name: gene neighbors evidence used in automatic assertion +def: "A type of gene neighbors evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000084 ! gene neighbors evidence +is_a: ECO:0007327 ! genomic context evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007334 +name: Edman degradation evidence used in automatic assertion +def: "A type of Edman degradation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001044 ! Edman degradation evidence +is_a: ECO:0007819 ! protein detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007335 +name: 3D cell culture evidence used in automatic assertion +def: "A type of 3D cell culture evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001001 ! 3D cell culture evidence +is_a: ECO:0007775 ! cell proliferation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007336 +name: 51Cr release assay evidence used in automatic assertion +def: "A type of 51Cr release assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001004 ! 51Cr release assay evidence +is_a: ECO:0007781 ! cytotoxicity assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007337 +name: 7-aminoactinomycin staining evidence used in automatic assertion +def: "A type of 7-aminoactinomycin staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001005 ! 7-aminoactinomycin staining evidence +is_a: ECO:0007798 ! staining evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007338 +name: [3H]-thymidine incorporation assay evidence used in automatic assertion +def: "A type of [3H]-thymidine incorporation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001003 ! [3H]-thymidine incorporation assay evidence +is_a: ECO:0007776 ! DNA synthesis cell proliferation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007339 +name: [3H]arachidonic acid release assay evidence used in automatic assertion +def: "A type of [3H]arachidonic acid release assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001002 ! [3H]arachidonic acid release assay evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007340 +name: adhesion assay evidence used in automatic assertion +def: "A type of adhesion assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001006 ! adhesion assay evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007341 +name: adoptive cell transfer evidence used in automatic assertion +def: "A type of adoptive cell transfer evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001007 ! adoptive cell transfer evidence +is_a: ECO:0007793 ! ex vivo assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007342 +name: alamarBlue assay evidence used in automatic assertion +def: "A type of alamarBlue assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001008 ! alamarBlue assay evidence +is_a: ECO:0007774 ! cell viability assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007343 +name: allograft transplantation phenotypic evidence used in automatic assertion +def: "A type of allograft transplantation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "allograft transplantation evidence used in automatic assertion" EXACT [] +is_a: ECO:0001009 ! allograft transplantation phenotypic evidence +is_a: ECO:0007810 ! anatomical perturbation phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007344 +name: anion-exchange chromatography evidence used in automatic assertion +def: "A type of anion-exchange chromatography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001010 ! anion-exchange chromatography evidence +is_a: ECO:0007681 ! chromatography evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007345 +name: annexin-V staining evidence used in automatic assertion +def: "A type of annexin-V staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001011 ! annexin-V staining evidence +is_a: ECO:0007777 ! apoptotic assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007346 +name: cognitive assay phenotypic evidence used in automatic assertion +def: "A type of cognitive assay phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "behavioral assay evidence used in automatic assertion" EXACT [] +is_a: ECO:0001012 ! cognitive assay phenotypic evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007347 +name: blocking monoclonal antibody evidence used in automatic assertion +def: "A type of blocking monoclonal antibody evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001013 ! blocking monoclonal antibody evidence +is_a: ECO:0007348 ! immunological assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007348 +name: immunological assay evidence used in automatic assertion +def: "A type of immunological assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000040 ! immunological assay evidence +is_a: ECO:0007384 ! affinity evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007349 +name: blocking peptide evidence used in automatic assertion +def: "A type of blocking peptide evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001014 ! blocking peptide evidence +is_a: ECO:0007348 ! immunological assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007350 +name: blocking polyclonal antibody evidence used in automatic assertion +def: "A type of blocking polyclonal antibody evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001015 ! blocking polyclonal antibody evidence +is_a: ECO:0007348 ! immunological assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007351 +name: blood test evidence used in automatic assertion +def: "A type of blood test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001016 ! blood test evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007352 +name: Boyden chamber assay evidence used in automatic assertion +def: "A type of Boyden chamber assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001017 ! Boyden chamber assay evidence +is_a: ECO:0007780 ! chemotaxis assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007353 +name: bromodeoxyuridine incorporation assay evidence used in automatic assertion +def: "A type of bromodeoxyuridine incorporation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001018 ! bromodeoxyuridine incorporation assay evidence +is_a: ECO:0007354 ! nucleotide analog incorporation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007354 +name: nucleotide analog incorporation assay evidence used in automatic assertion +def: "A type of nucleotide analog incorporation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001107 ! nucleotide analog incorporation assay evidence +is_a: ECO:0007776 ! DNA synthesis cell proliferation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007355 +name: caspase assay evidence used in automatic assertion +def: "A type of caspase assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001019 ! caspase assay evidence +is_a: ECO:0007777 ! apoptotic assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007356 +name: cell counting evidence used in automatic assertion +def: "A type of cell counting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001020 ! cell counting evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007357 +name: cell permeability assay evidence used in automatic assertion +def: "A type of cell permeability assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001021 ! cell permeability assay evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007358 +name: carboxyfluorescein diacetate succinimidyl ester staining evidence used in automatic assertion +def: "A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001022 ! carboxyfluorescein diacetate succinimidyl ester staining evidence +is_a: ECO:0007798 ! staining evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007359 +name: chemiluminescence-linked immunoassay evidence used in automatic assertion +def: "A type of chemiluminescence-linked immunoassay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001023 ! chemiluminescence-linked immunoassay evidence +is_a: ECO:0007819 ! protein detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007360 +name: chimeric protein phenotypic evidence used in automatic assertion +def: "A type of chimeric protein phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "chimeric protein evidence used in automatic assertion" EXACT [] +is_a: ECO:0001024 ! chimeric protein phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007361 +name: co-electrophoresis evidence used in automatic assertion +def: "A type of co-electrophoresis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001025 ! co-electrophoresis evidence +is_a: ECO:0007769 ! gel electrophoresis evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007362 +name: co-localization evidence used in automatic assertion +def: "A type of co-localization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001026 ! co-localization evidence +is_a: ECO:0007363 ! imaging assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007363 +name: imaging assay evidence used in automatic assertion +def: "A type of imaging assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000324 ! imaging assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007364 +name: co-sedimentation assay evidence used in automatic assertion +def: "A type of co-sedimentation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001028 ! co-sedimentation assay evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007365 +name: colony counting evidence used in automatic assertion +def: "A type of colony counting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001027 ! colony counting evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007366 +name: comet assay evidence used in automatic assertion +def: "A type of comet assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001029 ! comet assay evidence +is_a: ECO:0007769 ! gel electrophoresis evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007367 +name: conditional knockin evidence used in automatic assertion +def: "A type of conditional knockin evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001031 ! conditional knockin evidence +is_a: ECO:0007368 ! knockin evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007368 +name: knockin evidence used in automatic assertion +def: "A type of knockin evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001090 ! knockin evidence +is_a: ECO:0007427 ! genetic transformation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007369 +name: conditional knockout evidence used in automatic assertion +def: "A type of conditional knockout evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001030 ! conditional knockout evidence +is_a: ECO:0007370 ! knockout evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007370 +name: knockout evidence used in automatic assertion +def: "A type of knockout evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001091 ! knockout phenotypic evidence +is_a: ECO:0007427 ! genetic transformation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007371 +name: constitutively active mutant evidence used in automatic assertion +def: "A type of constitutively active mutant evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001032 ! constitutively active mutant evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007372 +name: cross-linking evidence used in automatic assertion +def: "A type of cross-linking evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001033 ! cross-linking evidence +is_a: ECO:0007373 ! protein binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007373 +name: protein binding evidence used in automatic assertion +def: "A type of protein binding evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000024 ! protein binding evidence +is_a: ECO:0007384 ! affinity evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007374 +name: crystallography evidence used in automatic assertion +def: "A type of crystallography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001034 ! crystallography evidence +is_a: ECO:0007815 ! structure determination evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007375 +name: cytochemistry evidence used in automatic assertion +def: "A type of cytochemistry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001035 ! cytochemistry evidence +is_a: ECO:0007376 ! histochemistry evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007376 +name: histochemistry evidence used in automatic assertion +def: "A type of histochemistry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001050 ! histochemistry evidence +is_a: ECO:0007389 ! histology evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007377 +name: cytochrome C release assay evidence used in automatic assertion +def: "A type of cytochrome C release assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001036 ! cytochrome C release assay evidence +is_a: ECO:0007777 ! apoptotic assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007378 +name: 4',6-diamidino-2-phenylindole staining evidence used in automatic assertion +def: "A type of 4',6-diamidino-2-phenylindole staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001037 ! 4',6-diamidino-2-phenylindole staining evidence +is_a: ECO:0007798 ! staining evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007379 +name: deletion mutation phenotypic evidence used in automatic assertion +def: "A type of deletion mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "deletion mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0001038 ! deletion mutation phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007380 +name: DNA laddering assay evidence used in automatic assertion +def: "A type of DNA laddering assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001039 ! DNA laddering assay evidence +is_a: ECO:0007777 ! apoptotic assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007381 +name: RNA dot blot assay evidence used in automatic assertion +def: "A type of RNA dot blot assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001040 ! RNA dot blot assay evidence +is_a: ECO:0007818 ! RNA detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007382 +name: dominant-negative mutant phenotypic evidence used in automatic assertion +def: "A type of dominant-negative mutant phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "dominant-negative mutant evidence used in automatic assertion" EXACT [] +is_a: ECO:0001042 ! dominant-negative mutant phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007383 +name: eTag assay evidence used in automatic assertion +def: "A type of eTag assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001045 ! eTag assay evidence +is_a: ECO:0007384 ! affinity evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007384 +name: affinity evidence used in automatic assertion +def: "A type of affinity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000023 ! affinity evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007385 +name: filter binding assay evidence used in automatic assertion +def: "A type of filter binding assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001046 ! filter binding assay evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007386 +name: fluorescence in situ hybridization evidence used in automatic assertion +def: "A type of fluorescence in situ hybridization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001047 ! fluorescence in situ hybridization evidence +is_a: ECO:0001837 ! in situ hybridization evidence used in automatic assertion +is_a: ECO:0007809 ! nucleic acid localization evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007387 +name: fluorescence resonance energy transfer evidence used in automatic assertion +def: "A type of fluorescence resonance energy transfer evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001048 ! fluorescence resonance energy transfer evidence +is_a: ECO:0006328 ! dynamic fluorescence quenching evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007388 +name: gel-filtration evidence used in automatic assertion +def: "A type of gel-filtration evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001049 ! gel-filtration evidence +is_a: ECO:0007681 ! chromatography evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007389 +name: histology evidence used in automatic assertion +def: "A type of histology evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001051 ! histology evidence +is_a: ECO:0007363 ! imaging assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007390 +name: immunocytochemistry evidence used in automatic assertion +def: "A type of immunocytochemistry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001053 ! immunocytochemistry evidence +is_a: ECO:0007819 ! protein detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007391 +name: immunodepletion evidence used in automatic assertion +def: "A type of immunodepletion evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001054 ! immunodepletion evidence +is_a: ECO:0007348 ! immunological assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007392 +name: immunohistochemistry evidence used in automatic assertion +def: "A type of immunohistochemistry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001055 ! immunohistochemistry evidence +is_a: ECO:0007819 ! protein detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007393 +name: in vitro acetylation assay evidence used in automatic assertion +def: "A type of in vitro acetylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001057 ! in vitro acetylation assay evidence +is_a: ECO:0007794 ! acetylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007394 +name: in vitro cleavage assay evidence used in automatic assertion +def: "A type of in vitro cleavage assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001058 ! in vitro cleavage assay evidence +is_a: ECO:0007782 ! cleavage assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007395 +name: in vitro deacetylation assay evidence used in automatic assertion +def: "A type of in vitro deacetylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001060 ! in vitro deacetylation assay evidence +is_a: ECO:0007771 ! deacetylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007396 +name: in vitro defarnesylation assay evidence used in automatic assertion +def: "A type of in vitro defarnesylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001061 ! in vitro defarnesylation assay evidence +is_a: ECO:0007790 ! defarnesylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007397 +name: in vitro demethylation assay evidence used in automatic assertion +def: "A type of in vitro demethylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001062 ! in vitro demethylation assay evidence +is_a: ECO:0007795 ! demethylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007398 +name: in vitro desumoylation assay evidence used in automatic assertion +def: "A type of in vitro desumoylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001063 ! in vitro desumoylation assay evidence +is_a: ECO:0007802 ! desumoylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007399 +name: in vitro deubiquitination assay evidence used in automatic assertion +def: "A type of in vitro deubiquitination assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001059 ! in vitro deubiquitination assay evidence +is_a: ECO:0007791 ! deubiquitination assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007400 +name: in vitro farnesylation assay evidence used in automatic assertion +def: "A type of in vitro farnesylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001064 ! in vitro farnesylation assay evidence +is_a: ECO:0007800 ! farnesylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007401 +name: in vitro methylation assay evidence used in automatic assertion +def: "A type of in vitro methylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001065 ! in vitro methylation assay evidence +is_a: ECO:0007783 ! methylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007402 +name: in vitro palmitoylation assay evidence used in automatic assertion +def: "A type of in vitro palmitoylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001066 ! in vitro palmitoylation assay evidence +is_a: ECO:0007792 ! palmitoylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007403 +name: in vitro phosphatase assay evidence used in automatic assertion +def: "A type of in vitro phosphatase assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001067 ! in vitro phosphatase assay evidence +is_a: ECO:0007772 ! phosphatase assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007404 +name: in vitro polyADP-ribosylation assay evidence used in automatic assertion +def: "A type of in vitro polyADP-ribosylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001069 ! in vitro polyADP-ribosylation assay evidence +is_a: ECO:0007797 ! polyADP-ribosylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007405 +name: in vitro protein kinase assay evidence used in automatic assertion +def: "A type of in vitro protein kinase assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001068 ! in vitro protein kinase assay evidence +is_a: ECO:0007767 ! protein kinase assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007406 +name: in vitro sumoylation assay evidence used in automatic assertion +def: "A type of in vitro sumoylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001070 ! in vitro sumoylation assay evidence +is_a: ECO:0007788 ! sumoylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007407 +name: in vitro transcription assay evidence used in automatic assertion +def: "A type of in vitro transcription assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001071 ! in vitro transcription assay evidence +is_a: ECO:0007768 ! transcription assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007408 +name: in vitro translation assay evidence used in automatic assertion +def: "A type of in vitro translation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001072 ! in vitro translation assay evidence +is_a: ECO:0007799 ! translation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007409 +name: in vitro ubiquitination assay evidence used in automatic assertion +def: "A type of in vitro ubiquitination assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001073 ! in vitro ubiquitination assay evidence +is_a: ECO:0007801 ! ubiquitination assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007410 +name: in vivo acetylation assay evidence used in automatic assertion +def: "A type of in vivo acetylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001074 ! in vivo acetylation assay evidence +is_a: ECO:0007794 ! acetylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007411 +name: in vivo cleavage assay evidence used in automatic assertion +def: "A type of in vivo cleavage assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001075 ! in vivo cleavage assay evidence +is_a: ECO:0007782 ! cleavage assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007412 +name: in vivo deacetylation assay evidence used in automatic assertion +def: "A type of in vivo deacetylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001076 ! in vivo deacetylation assay evidence +is_a: ECO:0007771 ! deacetylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007413 +name: in vivo defarnesylation assay evidence used in automatic assertion +def: "A type of in vivo defarnesylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001077 ! in vivo defarnesylation assay evidence +is_a: ECO:0007790 ! defarnesylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007414 +name: in vivo demethylation assay evidence used in automatic assertion +def: "A type of in vivo demethylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001078 ! in vivo demethylation assay evidence +is_a: ECO:0007795 ! demethylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007415 +name: in vivo desumoylation assay evidence used in automatic assertion +def: "A type of in vivo desumoylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001080 ! in vivo desumoylation assay evidence +is_a: ECO:0007802 ! desumoylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007416 +name: in vivo deubiquitination assay evidence used in automatic assertion +def: "A type of in vivo deubiquitination assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001079 ! in vivo deubiquitination assay evidence +is_a: ECO:0007791 ! deubiquitination assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007417 +name: in vivo farnesylation assay evidence used in automatic assertion +def: "A type of in vivo farnesylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001081 ! in vivo farnesylation assay evidence +is_a: ECO:0007800 ! farnesylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007418 +name: in vivo methylation assay evidence used in automatic assertion +def: "A type of in vivo methylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001082 ! in vivo methylation assay evidence +is_a: ECO:0007783 ! methylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007419 +name: in vivo palmitoylation assay evidence used in automatic assertion +def: "A type of in vivo palmitoylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001083 ! in vivo palmitoylation assay evidence +is_a: ECO:0007792 ! palmitoylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007420 +name: in vivo phosphatase assay evidence used in automatic assertion +def: "A type of in vivo phosphatase assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001084 ! in vivo phosphatase assay evidence +is_a: ECO:0007772 ! phosphatase assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007421 +name: in vivo protein kinase assay evidence used in automatic assertion +def: "A type of in vivo protein kinase assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001085 ! in vivo protein kinase assay evidence +is_a: ECO:0007767 ! protein kinase assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007422 +name: in vivo sumoylation assay evidence used in automatic assertion +def: "A type of in vivo sumoylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001086 ! in vivo sumoylation assay evidence +is_a: ECO:0007788 ! sumoylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007423 +name: in vivo transcription assay evidence used in automatic assertion +def: "A type of in vivo transcription assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001087 ! in vivo transcription assay evidence +is_a: ECO:0007768 ! transcription assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007424 +name: in vivo translation assay evidence used in automatic assertion +def: "A type of in vivo translation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001088 ! in vivo translation assay evidence +is_a: ECO:0007799 ! translation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007425 +name: in vivo ubiquitination assay evidence used in automatic assertion +def: "A type of in vivo ubiquitination assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001089 ! in vivo ubiquitination assay evidence +is_a: ECO:0007801 ! ubiquitination assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007426 +name: induced mutation evidence used in automatic assertion +def: "A type of induced mutation evidence that is used in an automatic assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007427 +name: genetic transformation evidence used in automatic assertion +def: "A type of genetic transformation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005027 ! genetic transformation evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007428 +name: lipid binding assay evidence used in automatic assertion +def: "A type of lipid binding assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001092 ! lipid binding assay evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007429 +name: luminescence-based mammalian interactome mapping assay evidence used in automatic assertion +def: "A type of luminescence-based mammalian interactome mapping assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001093 ! luminescence-based mammalian interactome mapping assay evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007430 +name: macroscopy evidence used in automatic assertion +def: "A type of macroscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001094 ! macroscopy evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007431 +name: mammalian 2-hybrid assay evidence used in automatic assertion +def: "A type of mammalian 2-hybrid assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001095 ! mammalian 2-hybrid assay evidence +is_a: ECO:0007432 ! bait-prey hybrid interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007432 +name: bait-prey hybrid interaction evidence used in automatic assertion +def: "A type of bait-prey hybrid interaction evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "bait-prey protein pull-down evidence" EXACT [] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence +is_a: ECO:0007373 ! protein binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007433 +name: mass spectrometry evidence used in automatic assertion +def: "A type of mass spectrometry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001096 ! mass spectrometry evidence +is_a: ECO:0007821 ! spectrometry evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007434 +name: medical imaging evidence used in automatic assertion +def: "A type of medical imaging evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001097 ! medical imaging evidence +is_a: ECO:0007363 ! imaging assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007435 +name: microscopy evidence used in automatic assertion +def: "A type of microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001098 ! microscopy evidence +is_a: ECO:0007363 ! imaging assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007436 +name: motility wound healing assay evidence used in automatic assertion +def: "A type of motility wound healing assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001099 ! motility wound healing assay evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007437 +name: MTS assay evidence used in automatic assertion +def: "A type of MTS assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001100 ! MTS assay evidence +is_a: ECO:0007777 ! apoptotic assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007438 +name: MTT assay evidence used in automatic assertion +def: "A type of MTT assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001101 ! MTT assay evidence +is_a: ECO:0007777 ! apoptotic assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007439 +name: multiplex bead-based immunoassay evidence used in automatic assertion +def: "A type of multiplex bead-based immunoassay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001102 ! multiplex bead-based immunoassay evidence +is_a: ECO:0007819 ! protein detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007440 +name: natural variation mutant evidence used in automatic assertion +def: "A type of natural variation mutant evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001103 ! natural variation mutant evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007441 +name: nuclear magnetic resonance evidence used in automatic assertion +def: "A type of nuclear magnetic resonance evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001105 ! nuclear magnetic resonance evidence +is_a: ECO:0006286 ! magnetic resonance evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007442 +name: nuclear fragmentation evidence used in automatic assertion +def: "A type of nuclear fragmentation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001104 ! nuclear fragmentation evidence +is_a: ECO:0007777 ! apoptotic assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007443 +name: phage display evidence used in automatic assertion +def: "A type of phage display evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001108 ! phage display evidence +is_a: ECO:0007373 ! protein binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007444 +name: phosphoamino acid analysis evidence used in automatic assertion +def: "A type of phosphoamino acid analysis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001109 ! phosphoamino acid analysis evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007445 +name: peptide affinity enrichment evidence used in automatic assertion +def: "A type of peptide affinity enrichment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001110 ! peptide affinity enrichment evidence +is_a: ECO:0007384 ! affinity evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007446 +name: peptide array evidence used in automatic assertion +def: "A type of peptide array evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001112 ! peptide array evidence +is_a: ECO:0007373 ! protein binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007447 +name: physical examination evidence used in automatic assertion +def: "A type of physical examination evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001111 ! physical examination evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007448 +name: point mutation phenotypic evidence used in automatic assertion +def: "A type of point mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "point mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0001113 ! point mutation phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007449 +name: propidium iodide staining evidence used in automatic assertion +def: "A type of propidium iodide staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001114 ! propidium iodide staining evidence +is_a: ECO:0007798 ! staining evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007450 +name: fluorescence evidence used in automatic assertion +def: "A type of fluorescence evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001115 ! fluorescence evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007451 +name: protein dot blot assay evidence used in automatic assertion +def: "A type of protein dot blot assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001116 ! protein dot blot assay evidence +is_a: ECO:0007819 ! protein detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007452 +name: protein microarray evidence used in automatic assertion +def: "A type of protein microarray evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001117 ! protein microarray evidence +is_a: ECO:0007819 ! protein detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007453 +name: protein sequencing assay evidence used in automatic assertion +def: "A type of protein sequencing assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001118 ! protein sequencing assay evidence +is_a: ECO:0007812 ! sequencing assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007454 +name: quantitative mass spectrometry evidence used in automatic assertion +def: "A type of quantitative mass spectrometry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001119 ! quantitative mass spectrometry evidence +is_a: ECO:0007433 ! mass spectrometry evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007455 +name: radioisotope assay evidence used in automatic assertion +def: "A type of radioisotope assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001120 ! radioisotope assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007456 +name: radioimmunoassay evidence used in automatic assertion +def: "A type of radioimmunoassay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001121 ! radioimmunoassay evidence +is_a: ECO:0007819 ! protein detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007457 +name: restriction fragment detection evidence used in automatic assertion +def: "A type of restriction fragment detection evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001124 ! restriction fragment detection evidence +is_a: ECO:0007817 ! DNA detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007458 +name: spectrophotometry evidence used in automatic assertion +def: "A type of spectrophotometry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001126 ! spectrophotometry evidence +is_a: ECO:0007821 ! spectrometry evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007459 +name: syngeneic transplantation experiment evidence used in automatic assertion +def: "A type of syngeneic transplantation experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001128 ! syngeneic transplantation experiment evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007460 +name: xenotransplantation phenotypic evidence used in automatic assertion +def: "A type of xenotransplantation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "xenotransplantation experiment evidence used in automatic assertion" EXACT [] +is_a: ECO:0001137 ! xenotransplantation phenotypic evidence +is_a: ECO:0007810 ! anatomical perturbation phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007461 +name: WST-1 assay evidence used in automatic assertion +def: "A type of WST-1 assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001136 ! WST-1 assay evidence +is_a: ECO:0007775 ! cell proliferation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007462 +name: urine test evidence used in automatic assertion +def: "A type of urine test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001134 ! urine test evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007463 +name: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in automatic assertion +def: "A type of terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001133 ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence +is_a: ECO:0007777 ! apoptotic assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007464 +name: tryptic phosphopeptide mapping assay evidence used in automatic assertion +def: "A type of tryptic phosphopeptide mapping assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001132 ! tryptic phosphopeptide mapping assay evidence +is_a: ECO:0007433 ! mass spectrometry evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007465 +name: transgenic organism evidence used in automatic assertion +def: "A type of transgenic organism evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001131 ! transgenic organism evidence +is_a: ECO:0007427 ! genetic transformation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007466 +name: tissue microarray evidence used in automatic assertion +def: "A type of tissue microarray evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001130 ! tissue microarray evidence +is_a: ECO:0007375 ! cytochemistry evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007467 +name: TACE activity assay evidence used in automatic assertion +def: "A type of TACE activity assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001129 ! TACE activity assay evidence +is_a: ECO:0007468 ! enzymatic activity assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007468 +name: enzymatic activity assay evidence used in automatic assertion +def: "A type of enzyme assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000005 ! enzymatic activity assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007469 +name: surface plasmon resonance evidence used in automatic assertion +def: "A type of surface plasmon resonance evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001127 ! surface plasmon resonance evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007470 +name: restriction landmark genomic scanning evidence used in automatic assertion +def: "A type of restriction landmark genomic scanning evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000089 ! restriction landmark genomic scanning evidence +is_a: ECO:0007817 ! DNA detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007471 +name: resonant mirror biosensor evidence used in automatic assertion +def: "A type of resonant mirror biosensor evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001123 ! resonant mirror biosensor evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007472 +name: high-performance liquid chromatography evidence used in automatic assertion +def: "A type of high-performance liquid chromatography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001052 ! high-performance liquid chromatography evidence +is_a: ECO:0007681 ! chromatography evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007473 +name: ectopic expression evidence used in automatic assertion +def: "A type of ectopic expression evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000017 ! ectopic expression evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007474 +name: electrophoretic mobility shift assay evidence used in automatic assertion +def: "A type of electrophoretic mobility shift assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000096 ! electrophoretic mobility shift assay evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007475 +name: reverse transcription polymerase chain reaction evidence used in automatic assertion +alt_id: ECO:000108 +def: "A type of reverse transcription polymerase chain reaction evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000109 ! reverse transcription polymerase chain reaction evidence +is_a: ECO:0007818 ! RNA detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007476 +name: in vivo polyADP-ribosylation assay evidence used in automatic assertion +def: "A type of in vivo polyADP-ribosylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005500 ! in vivo polyADP-ribosylation assay evidence +is_a: ECO:0007797 ! polyADP-ribosylation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007477 +name: DNA dot blot assay evidence used in automatic assertion +def: "A type of DNA dot blot assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005522 ! DNA dot blot assay evidence +is_a: ECO:0007817 ! DNA detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007478 +name: random mutagenesis evidence used in automatic assertion +def: "A type of random mutagenesis phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005529 ! random mutagenesis phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007479 +name: biological system reconstruction evidence by experimental evidence from single species used in automatic assertion +def: "A type of biological system reconstruction evidence by experimental evidence from single species that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005553 ! biological system reconstruction evidence by experimental evidence from single species +is_a: ECO:0007828 ! biological system reconstruction evidence by experimental evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007480 +name: biological system reconstruction evidence by experimental evidence from mixed species used in automatic assertion +def: "A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005552 ! biological system reconstruction evidence by experimental evidence from mixed species +is_a: ECO:0007828 ! biological system reconstruction evidence by experimental evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007481 +name: biological system reconstruction evidence based on orthology evidence used in automatic assertion +def: "A type of biological system reconstruction evidence based on orthology evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005545 ! biological system reconstruction evidence based on orthology evidence +is_a: ECO:0007482 ! biological system reconstruction evidence based on homology evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007482 +name: biological system reconstruction evidence based on homology evidence used in automatic assertion +def: "A type of biological system reconstruction evidence based on homology evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence +is_a: ECO:0007827 ! biological system reconstruction evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007483 +name: biological system reconstruction evidence based on paralogy evidence used in automatic assertion +def: "A type of biological system reconstruction evidence based on paralogy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005550 ! biological system reconstruction evidence based on paralogy evidence +is_a: ECO:0007482 ! biological system reconstruction evidence based on homology evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007484 +name: biological system reconstruction evidence based on inference from background scientific knowledge used in automatic assertion +def: "A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005548 ! biological system reconstruction evidence based on inference from background scientific knowledge +is_a: ECO:0007827 ! biological system reconstruction evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007485 +name: immunogold labelling evidence used in automatic assertion +def: "A type of immunogold labelling evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000090 ! immunogold labelling evidence +is_a: ECO:0007486 ! immunolocalization evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007486 +name: immunolocalization evidence used in automatic assertion +def: "A type of immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000087 ! immunolocalization evidence +is_a: ECO:0007435 ! microscopy evidence used in automatic assertion +is_a: ECO:0007796 ! immunodetection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007487 +name: flow cytometry evidence used in automatic assertion +def: "A type of flow cytometry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000268 ! flow cytometry evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007488 +name: enzyme-linked immunoabsorbent assay evidence used in automatic assertion +def: "A type of enzyme-linked immunoabsorbent assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000267 ! enzyme-linked immunoabsorbent assay evidence +is_a: ECO:0007819 ! protein detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007489 +name: high throughput mass spectrometry evidence used in automatic assertion +def: "A type of high throughput mass spectrometry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005585 ! high throughput mass spectrometry evidence +is_a: ECO:0007433 ! mass spectrometry evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007490 +name: confocal microscopy evidence used in automatic assertion +def: "A type of confocal microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005587 ! confocal microscopy evidence +is_a: ECO:0007435 ! microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007491 +name: wide-field microscopy evidence used in automatic assertion +def: "A type of wide-field microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005588 ! wide-field microscopy evidence +is_a: ECO:0007435 ! microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007492 +name: immunogold labelling electron microscopy assay evidence used in automatic assertion +def: "A type of immunogold labelling electron microscopy assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005591 ! immunogold labelling electron microscopy assay evidence +is_a: ECO:0007485 ! immunogold labelling evidence used in automatic assertion +is_a: ECO:0007493 ! electron microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007493 +name: electron microscopy evidence used in automatic assertion +def: "A type of electron microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005033 ! electron microscopy evidence +is_a: ECO:0007435 ! microscopy evidence used in automatic assertion +is_a: ECO:0007815 ! structure determination evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007494 +name: immunoperoxidase immunolocalization evidence used in automatic assertion +def: "A type of immunoperoxidase immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005594 ! immunoperoxidase immunolocalization evidence +is_a: ECO:0007486 ! immunolocalization evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007495 +name: immunoperoxidase immunolocalization electron microscopy evidence used in automatic assertion +def: "A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005596 ! immunoperoxidase immunolocalization electron microscopy evidence +is_a: ECO:0007493 ! electron microscopy evidence used in automatic assertion +is_a: ECO:0007494 ! immunoperoxidase immunolocalization evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007496 +name: immunofluorescence confocal microscopy evidence used in automatic assertion +def: "A type of immunofluorescence confocal microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005600 ! immunofluorescence confocal microscopy evidence +is_a: ECO:0007490 ! confocal microscopy evidence used in automatic assertion +is_a: ECO:0007497 ! immunofluorescence evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007497 +name: immunofluorescence evidence used in automatic assertion +def: "A type of immunofluorescence evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000007 ! immunofluorescence evidence +is_a: ECO:0007819 ! protein detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007498 +name: two-dimensional polyacrylamide gel electrophoresis evidence used in automatic assertion +def: "A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005503 ! two-dimensional polyacrylamide gel electrophoresis evidence +is_a: ECO:0007769 ! gel electrophoresis evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007499 +name: alkaline phosphatase reporter gene assay evidence used in automatic assertion +def: "A type of alkaline phosphatase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001801 ! alkaline phosphatase reporter gene assay evidence +is_a: ECO:0007765 ! reporter gene assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007500 +name: beta-galactosidase reporter gene assay evidence used in automatic assertion +def: "A type of beta-galactosidase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "LacZ transcript localization evidence used in automatic assertion" EXACT [] +is_a: ECO:0001802 ! beta-galactosidase reporter gene assay evidence +is_a: ECO:0007765 ! reporter gene assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007501 +name: chloramphenicol acetyltransferase reporter gene assay evidence used in automatic assertion +def: "A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001803 ! chloramphenicol acetyltransferase reporter gene assay evidence +is_a: ECO:0007765 ! reporter gene assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007502 +name: chromatin immunoprecipitation-PCR evidence used in automatic assertion +def: "A type of chromatin immunoprecipitation-PCR evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000227 ! chromatin immunoprecipitation-PCR evidence +is_a: ECO:0007786 ! chromatin immunoprecipitation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007503 +name: immunoprecipitation evidence used in automatic assertion +def: "A type of immunoprecipitation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000085 ! immunoprecipitation evidence +is_a: ECO:0007373 ! protein binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007504 +name: copper-phenanthroline footprinting evidence used in automatic assertion +def: "A type of copper-phenanthroline footprinting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001815 ! copper-phenanthroline footprinting evidence +is_a: ECO:0007505 ! nucleic acid binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007505 +name: nucleic acid binding evidence used in automatic assertion +def: "A type of nucleic acid binding evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000136 ! nucleic acid binding evidence +is_a: ECO:0007384 ! affinity evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007506 +name: DNA affinity chromatography evidence used in automatic assertion +def: "A type of DNA affinity chromatography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001809 ! DNA affinity chromatography evidence +is_a: ECO:0007681 ! chromatography evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007507 +name: DNAse footprinting evidence used in automatic assertion +def: "A type of DNAse footprinting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001810 ! DNAse footprinting evidence +is_a: ECO:0007505 ! nucleic acid binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007508 +name: fluorescence anisotropy evidence used in automatic assertion +def: "A type of fluorescence anisotropy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001811 ! fluorescence anisotropy evidence +is_a: ECO:0007450 ! fluorescence evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007509 +name: ferric uptake regulator titration assay evidence used in automatic assertion +def: "A type of ferric uptake regulator titration assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001829 ! ferric uptake regulator titration assay evidence +is_a: ECO:0007765 ! reporter gene assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007510 +name: systematic evolution of ligands by exponential amplification evidence used in automatic assertion +def: "A type of systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007511 +name: glutathione S-transferase pull-down assay evidence used in automatic assertion +def: "A type of glutathione S-transferase pull-down assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001817 ! glutathione S-transferase pull-down assay evidence +is_a: ECO:0007432 ! bait-prey hybrid interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007512 +name: beta-glucuronidase reporter gene assay evidence used in automatic assertion +def: "A type of beta-glucuronidase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001804 ! beta-glucuronidase reporter gene assay evidence +is_a: ECO:0007765 ! reporter gene assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007513 +name: heteronuclear single quantum coherence spectroscopy evidence used in automatic assertion +def: "A type of heteronuclear single quantum coherence spectroscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001813 ! heteronuclear single quantum coherence spectroscopy evidence +is_a: ECO:0006164 ! nuclear magnetic resonance spectroscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007514 +name: hydroxyl-radical footprinting evidence used in automatic assertion +def: "A type of hydroxyl-radical footprinting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001818 ! hydroxyl-radical footprinting evidence +is_a: ECO:0007505 ! nucleic acid binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007515 +name: isothermal titration calorimetry evidence used in automatic assertion +def: "A type of isothermal titration calorimetry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001825 ! isothermal titration calorimetry evidence +is_a: ECO:0007384 ! affinity evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007516 +name: luciferase reporter gene assay evidence used in automatic assertion +def: "A type of luciferase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001805 ! luciferase reporter gene assay evidence +is_a: ECO:0007765 ! reporter gene assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007517 +name: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in automatic assertion +def: "A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001814 ! methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence +is_a: ECO:0007505 ! nucleic acid binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007518 +name: northern blot evidence used in automatic assertion +def: "A type of northern blot evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000106 ! northern blot evidence +is_a: ECO:0007303 ! transcript expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007519 +name: methylation interference footprinting evidence used in automatic assertion +def: "A type of methylation interference footprinting evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "premethylation interference footprinting evidence used in automatic assertion" EXACT [] +is_a: ECO:0001827 ! methylation interference footprinting evidence +is_a: ECO:0007505 ! nucleic acid binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007520 +name: primer extension assay evidence used in automatic assertion +def: "A type of primer extension assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001819 ! primer extension assay evidence +is_a: ECO:0007303 ! transcript expression evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007521 +name: quantitative polymerase chain reaction evidence used in automatic assertion +def: "A type of quantitative polymerase chain reaction evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000231 ! quantitative polymerase chain reaction evidence +is_a: ECO:0007817 ! DNA detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007522 +name: rapid amplification of cDNA ends polymerase chain reaction evidence used in automatic assertion +def: "A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001820 ! rapid amplification of cDNA ends polymerase chain reaction evidence +is_a: ECO:0007813 ! nucleotide sequencing assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007523 +name: S1 nuclease protection assay evidence used in automatic assertion +def: "A type of S1 nuclease protection assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005521 ! S1 nuclease protection assay evidence +is_a: ECO:0007316 ! nuclease protection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007524 +name: site-directed mutagenesis phenotypic evidence used in automatic assertion +def: "A type of site-directed mutagenesis phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "site-directed mutagenesis evidence used in automatic assertion" EXACT [] +is_a: ECO:0005528 ! site-directed mutagenesis phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007525 +name: survival rate analysis evidence used in automatic assertion +def: "A type of survival rate analysis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001822 ! survival rate analysis evidence +is_a: ECO:0007370 ! knockout evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007526 +name: ultraviolet light footprinting evidence used in automatic assertion +def: "A type of ultraviolet light footprinting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001826 ! ultraviolet light footprinting evidence +is_a: ECO:0007505 ! nucleic acid binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007527 +name: xylE reporter gene assay evidence used in automatic assertion +def: "A type of xylE reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005537 ! xylE reporter gene assay evidence +is_a: ECO:0007765 ! reporter gene assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007528 +name: ad-hoc qualitative phenotype observation evidence used in automatic assertion +def: "A type of ad-hoc qualitative phenotype observation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005673 ! ad-hoc qualitative phenotype observation evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007529 +name: ad-hoc quantitative phenotype observation evidence used in automatic assertion +def: "A type of ad-hoc quantitative phenotype observation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005675 ! ad-hoc quantitative phenotype observation evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007530 +name: cell transfection experiment evidence used in automatic assertion +def: "A type of cell transfection experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005606 ! cell transfection experiment evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007531 +name: yeast 2-hybrid evidence used in automatic assertion +def: "A type of yeast 2-hybrid evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000068 ! yeast 2-hybrid evidence +is_a: ECO:0007432 ! bait-prey hybrid interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007532 +name: Cya fusion reporter assay evidence used in automatic assertion +def: "A type of Cya fusion reporter assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006001 ! Cya fusion reporter assay evidence +is_a: ECO:0007473 ! ectopic expression evidence used in automatic assertion +is_a: ECO:0007765 ! reporter gene assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007533 +name: super-resolution microscopy evidence used in automatic assertion +def: "A type of super-resolution microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005578 ! super-resolution microscopy evidence +is_a: ECO:0007435 ! microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007534 +name: fractionation evidence used in automatic assertion +def: "A type of fractionation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000100 ! fractionation evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007535 +name: electrophysiology assay evidence used in automatic assertion +def: "A type of electrophysiology assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000164 ! electrophysiology assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007536 +name: mRNA interactome capture evidence used in automatic assertion +def: "A type of mRNA interactome capture evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006010 ! mRNA interactome capture evidence +is_a: ECO:0007505 ! nucleic acid binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007537 +name: patch-clamp recording evidence used in automatic assertion +def: "A type of patch-clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006012 ! patch-clamp recording evidence +is_a: ECO:0007535 ! electrophysiology assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007538 +name: whole-cell patch-clamp recording evidence used in automatic assertion +def: "A type of whole-cell patch-clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006014 ! whole-cell patch-clamp recording evidence +is_a: ECO:0007537 ! patch-clamp recording evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007539 +name: author statement from published clinical study used in automatic assertion +def: "A type of author statement from published clinical study that is used in an automatic assertion." [ECO:RCT] +synonym: "traceable author statement from published clinical study used in automatic assertion" RELATED [] +is_a: ECO:0006016 ! author statement from published clinical study +is_a: ECO:0007321 ! author statement supported by traceable reference used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007540 +name: inference based on individual clinical experience used in automatic assertion +def: "A type of inference based on individual clinical experience that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006018 ! inference based on individual clinical experience +is_a: ECO:0007322 ! curator inference used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007541 +name: biofilm formation assay evidence used in automatic assertion +def: "A type of biofilm formation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006020 ! biofilm formation assay evidence +is_a: ECO:0007775 ! cell proliferation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007542 +name: microtiter plate biofilm assay evidence used in automatic assertion +def: "A type of microtiter plate biofilm assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006022 ! microtiter plate biofilm assay evidence +is_a: ECO:0007541 ! biofilm formation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007543 +name: air-liquid interface assay evidence used in automatic assertion +def: "A type of air-liquid interface assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006024 ! air-liquid interface assay evidence +is_a: ECO:0007541 ! biofilm formation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007544 +name: colony biofilm assay evidence used in automatic assertion +def: "A type of colony biofilm assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006026 ! colony biofilm assay evidence +is_a: ECO:0007541 ! biofilm formation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007545 +name: Kadouri drip-fed biofilm assay evidence used in automatic assertion +def: "A type of Kadouri drip-fed biofilm assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006028 ! Kadouri drip-fed biofilm assay evidence +is_a: ECO:0007541 ! biofilm formation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007546 +name: co-immunoprecipitation evidence used in automatic assertion +def: "A type of co-immunoprecipitation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000070 ! co-immunoprecipitation evidence +is_a: ECO:0007503 ! immunoprecipitation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007547 +name: optogenetic evidence used in automatic assertion +def: "A type of optogenetic evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006032 ! optogenetic evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007548 +name: fluorescent sensor evidence used in automatic assertion +def: "A type of fluorescent sensor evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006034 ! fluorescent sensor evidence +is_a: ECO:0007450 ! fluorescence evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007549 +name: genetically encoded fluorescent sensor evidence used in automatic assertion +def: "A type of genetically encoded fluorescent sensor evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006036 ! genetically encoded fluorescent sensor evidence +is_a: ECO:0007548 ! fluorescent sensor evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007550 +name: genetically encoded fluorescent electrophysiology assay evidence used in automatic assertion +def: "A type of genetically encoded fluorescent electrophysiology assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006038 ! genetically encoded fluorescent electrophysiology assay evidence +is_a: ECO:0007535 ! electrophysiology assay evidence used in automatic assertion +is_a: ECO:0007548 ! fluorescent sensor evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007551 +name: genetically encoded fluorescent ion concentration sensor assay evidence used in automatic assertion +def: "A type of genetically encoded fluorescent ion concentration sensor assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006040 ! genetically encoded fluorescent ion concentration sensor assay evidence +is_a: ECO:0007550 ! genetically encoded fluorescent electrophysiology assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007552 +name: cell fractionation evidence used in automatic assertion +def: "A type of cell fractionation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000004 ! cell fractionation evidence +is_a: ECO:0007534 ! fractionation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007553 +name: extracellular recording evidence used in automatic assertion +def: "A type of extracellular recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006043 ! extracellular recording evidence +is_a: ECO:0007535 ! electrophysiology assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007554 +name: single-unit extracellular recording evidence used in automatic assertion +def: "A type of single-unit extracellular recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006045 ! single-unit extracellular recording evidence +is_a: ECO:0007553 ! extracellular recording evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007555 +name: field potential recording evidence used in automatic assertion +def: "A type of field potential recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006047 ! field potential recording evidence +is_a: ECO:0007553 ! extracellular recording evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007556 +name: anti-sense experiment evidence used in automatic assertion +def: "A type of anti-sense experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000018 ! anti-sense experiment evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007557 +name: morpholino experiment evidence used in automatic assertion +def: "A type of morpholino experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000292 ! morpholino experiment evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007558 +name: RNAi evidence used in automatic assertion +def: "A type of RNAi evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000019 ! RNAi evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007559 +name: pharmacological assay evidence used in automatic assertion +def: "A type of pharmacological assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006053 ! pharmacological assay evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007560 +name: immunofluorescence wide-field microscopy evidence used in automatic assertion +def: "A type of immunofluorescence wide-field microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005599 ! immunofluorescence wide-field microscopy evidence +is_a: ECO:0007497 ! immunofluorescence evidence used in automatic assertion +is_a: ECO:0007561 ! wide-field fluorescence microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007561 +name: wide-field fluorescence microscopy evidence used in automatic assertion +def: "A type of wide-field fluorescence microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005598 ! wide-field fluorescence microscopy evidence +is_a: ECO:0007491 ! wide-field microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007562 +name: over expression analysis evidence used in automatic assertion +def: "A type of over expression analysis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000120 ! over expression analysis evidence +is_a: ECO:0007427 ! genetic transformation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007563 +name: cell-free assay evidence used in automatic assertion +def: "A type of cell-free assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000183 ! cell-free assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007564 +name: fluorescence recovery after photobleaching evidence used in automatic assertion +def: "A type of fluorescence recovery after photobleaching evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006066 ! fluorescence recovery after photobleaching evidence +is_a: ECO:0007450 ! fluorescence evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007565 +name: immuno-labelling electron microscopy evidence used in automatic assertion +def: "A type of immuno-labelling electron microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006070 ! immuno-labelling electron microscopy evidence +is_a: ECO:0007493 ! electron microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007566 +name: immunofluorescence super resolution microscopy evidence used in automatic assertion +def: "A type of immunofluorescence super resolution microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006072 ! immunofluorescence super resolution microscopy evidence +is_a: ECO:0007533 ! super-resolution microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007567 +name: co-purification evidence used in automatic assertion +def: "A type of co-purification evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000022 ! co-purification evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007568 +name: yeast one-hybrid evidence used in automatic assertion +def: "A type of yeast one-hybrid evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000066 ! yeast one-hybrid evidence +is_a: ECO:0007432 ! bait-prey hybrid interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007569 +name: split-ubiquitin functional complementation evidence used in automatic assertion +def: "A type of split-ubiquitin functional complementation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000074 ! split-ubiquitin functional complementation evidence +is_a: ECO:0006294 ! protein fragment functional complementation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007570 +name: far-Western blotting evidence used in automatic assertion +def: "A type of far-Western blotting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000076 ! far-Western blotting evidence +is_a: ECO:0007332 ! physical interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007571 +name: affinity chromatography evidence used in automatic assertion +def: "A type of affinity chromatography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000079 ! affinity chromatography evidence +is_a: ECO:0007384 ! affinity evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007572 +name: ribohomopolymer binding assay evidence used in automatic assertion +def: "A type of ribohomopolymer binding assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000138 ! ribohomopolymer binding assay evidence +is_a: ECO:0007505 ! nucleic acid binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007573 +name: protein:ion binding evidence used in automatic assertion +def: "A type of protein:ion binding evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000142 ! protein:ion binding evidence +is_a: ECO:0007373 ! protein binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007574 +name: Southwestern blot evidence used in automatic assertion +def: "A type of Southwestern blot evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000144 ! Southwestern blot evidence +is_a: ECO:0007505 ! nucleic acid binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007575 +name: Northwestern blot evidence used in automatic assertion +def: "A type of Northwestern blot evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000146 ! Northwestern blot evidence +is_a: ECO:0007505 ! nucleic acid binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007576 +name: bacterial one-hybrid evidence used in automatic assertion +def: "A type of bacterial one-hybrid evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000294 ! bacterial one-hybrid evidence +is_a: ECO:0007432 ! bait-prey hybrid interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007577 +name: protein-oligonucleotide microarray binding evidence used in automatic assertion +def: "A type of protein-oligonucleotide microarray binding evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001601 ! protein-oligonucleotide microarray binding evidence +is_a: ECO:0007373 ! protein binding evidence used in automatic assertion +is_a: ECO:0007505 ! nucleic acid binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007578 +name: functional complementation evidence used in automatic assertion +def: "A type of functional complementation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000012 ! functional complementation evidence +is_a: ECO:0007326 ! genetic interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007579 +name: transgenic rescue experiment evidence used in automatic assertion +def: "A type of transgenic rescue experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000013 ! transgenic rescue experiment evidence +is_a: ECO:0007578 ! functional complementation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007580 +name: transient rescue experiment evidence used in automatic assertion +def: "A type of transient rescue experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000038 ! transient rescue experiment evidence +is_a: ECO:0007578 ! functional complementation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007581 +name: suppressor/enhancer interaction phenotypic evidence used in automatic assertion +def: "A type of suppressor/enhancer interaction phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "suppressor/enhancer interaction evidence used in automatic assertion" EXACT [] +is_a: ECO:0000052 ! suppressor/enhancer interaction phenotypic evidence +is_a: ECO:0007326 ! genetic interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007582 +name: double mutant phenotypic evidence used in automatic assertion +def: "A type of double mutant phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "double mutant phenotype evidence used in automatic assertion" EXACT [] +is_a: ECO:0000054 ! double mutant phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007583 +name: epistatic interaction phenotypic evidence used in automatic assertion +def: "A type of epistatic interaction phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "epistatic interaction evidence used in automatic assertion" EXACT [] +is_a: ECO:0000056 ! epistatic interaction phenotypic evidence +is_a: ECO:0007326 ! genetic interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007584 +name: functional complementation in heterologous system evidence used in automatic assertion +def: "A type of functional complementation in heterologous system evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000064 ! functional complementation in heterologous system evidence +is_a: ECO:0007578 ! functional complementation evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007585 +name: temperature-sensitive mutant phenotypic evidence used in automatic assertion +def: "A type of temperature-sensitive mutant phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "temperature-sensitive mutant phenotype evidence used in automatic assertion" EXACT [] +is_a: ECO:0006098 ! temperature-sensitive mutant phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007586 +name: recessive mutant phenotype evidence used in automatic assertion +def: "A type of recessive mutant phenotype evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006100 ! recessive mutant phenotype evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007587 +name: high throughput mutant phenotypic evidence used in automatic assertion +def: "A type of high throughput mutant phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "high throughput mutant phenotype evidence used in automatic assertion" EXACT [] +is_a: ECO:0006057 ! high throughput evidence used in automatic assertion +is_a: ECO:0007000 ! high throughput mutant phenotypic evidence +is_a: ECO:0007325 ! mutant phenotype evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007588 +name: high throughput genetic interaction phenotypic evidence used in automatic assertion +def: "A type of high throughput genetic interaction phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "high throughput genetic interaction evidence used in automatic assertion" EXACT [] +is_a: ECO:0006057 ! high throughput evidence used in automatic assertion +is_a: ECO:0007002 ! high throughput genetic interaction phenotypic evidence +is_a: ECO:0007326 ! genetic interaction evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007589 +name: high throughput direct assay evidence used in automatic assertion +def: "A type of high throughput direct assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006057 ! high throughput evidence used in automatic assertion +is_a: ECO:0007004 ! high throughput direct assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007590 +name: high throughput expression pattern evidence used in automatic assertion +def: "A type of high throughput expression pattern evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006057 ! high throughput evidence used in automatic assertion +is_a: ECO:0007006 ! high throughput expression pattern evidence +is_a: ECO:0007298 ! expression pattern evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007591 +name: radioligand binding assay evidence used in automatic assertion +def: "A type of radioligand binding assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007008 ! radioligand binding assay evidence +is_a: ECO:0007373 ! protein binding evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007592 +name: combinatorial experimental and author inference evidence used in automatic assertion +def: "A type of combinatorial evidence from author knowledge and experimental evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "combinatorial evidence from author knowledge and experimental evidence used in automatic assertion" EXACT [] +is_a: ECO:0000213 ! combinatorial evidence used in automatic assertion +is_a: ECO:0007011 ! combinatorial experimental and author inference evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007593 +name: combinatorial experimental and curator inference evidence used in automatic assertion +def: "A type of combinatorial evidence from curator knowledge and experimental evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "combinatorial evidence from curator knowledge and experimental evidence used in automatic assertion" EXACT [] +is_a: ECO:0000213 ! combinatorial evidence used in automatic assertion +is_a: ECO:0007012 ! combinatorial experimental and curator inference evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007594 +name: voltammetry evidence used in automatic assertion +def: "A type of voltammetry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007015 ! voltammetry evidence +is_a: ECO:0007805 ! substance quantification evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007595 +name: photoconversion evidence used in automatic assertion +def: "A type of photoconversion evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007017 ! photoconversion evidence +is_a: ECO:0007450 ! fluorescence evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007596 +name: agglutination test evidence used in automatic assertion +def: "A type of agglutination test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007019 ! agglutination test evidence +is_a: ECO:0007348 ! immunological assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007597 +name: slide agglutination test evidence used in automatic assertion +def: "A type of slide agglutination test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007021 ! slide agglutination test evidence +is_a: ECO:0007596 ! agglutination test evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007598 +name: direct Coombs test evidence used in automatic assertion +def: "A type of direct Coombs test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007023 ! direct Coombs test evidence +is_a: ECO:0007596 ! agglutination test evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007599 +name: indirect Coombs test evidence used in automatic assertion +def: "A type of indirect Coombs test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007025 ! indirect Coombs test evidence +is_a: ECO:0007596 ! agglutination test evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007600 +name: direct hemagglutination assay evidence used in automatic assertion +def: "A type of direct hemagglutination assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007027 ! direct hemagglutination assay evidence +is_a: ECO:0007596 ! agglutination test evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007601 +name: viral hemagglutination inhibition assay evidence used in automatic assertion +def: "A type of viral hemagglutination inhibition assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007029 ! viral hemagglutination inhibition assay evidence +is_a: ECO:0007596 ! agglutination test evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007602 +name: compement fixation assay evidence used in automatic assertion +def: "A type of compement fixation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007031 ! compement fixation assay evidence +is_a: ECO:0007348 ! immunological assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007603 +name: neutralization test assay evidence used in automatic assertion +def: "A type of neutralization test assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007033 ! neutralization test assay evidence +is_a: ECO:0007348 ! immunological assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007604 +name: copper transport assay evidence used in automatic assertion +def: "A type of copper transport assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007035 ! copper transport assay evidence +is_a: ECO:0007789 ! transport assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007605 +name: 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence used in automatic assertion +def: "A type of 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007037 ! 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence +is_a: ECO:0007798 ! staining evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007606 +name: plaque assay evidence used in automatic assertion +def: "A type of plaque assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007039 ! plaque assay evidence +is_a: ECO:0007778 ! cell growth assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007607 +name: epifluorescence microscopy evidence used in automatic assertion +def: "A type of epifluorescence microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006324 ! fluorescence microscopy evidence used in automatic assertion +is_a: ECO:0007041 ! epifluorescence microscopy evidence +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007608 +name: transmission electron microscopy evidence used in automatic assertion +def: "A type of transmission electron microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007043 ! transmission electron microscopy evidence +is_a: ECO:0007493 ! electron microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007609 +name: scanning electron microscopy evidence used in automatic assertion +def: "A type of scanning electron microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007045 ! scanning electron microscopy evidence +is_a: ECO:0007493 ! electron microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007610 +name: time-lapsed microscopy evidence used in automatic assertion +def: "A type of time-lapsed microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007047 ! time-lapsed microscopy evidence +is_a: ECO:0007435 ! microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007611 +name: phase contrast microscopy evidence used in automatic assertion +def: "A type of phase contrast microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007049 ! phase contrast microscopy evidence +is_a: ECO:0007435 ! microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007612 +name: transmitted light brightfied mircoscopy evidence used in automatic assertion +def: "A type of transmitted light brightfied mircoscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007051 ! transmitted light brightfied mircoscopy evidence +is_a: ECO:0007435 ! microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007613 +name: koehler illumination microscopy evidence used in automatic assertion +def: "A type of koehler illumination microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007053 ! koehler illumination microscopy evidence +is_a: ECO:0007435 ! microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007614 +name: differential interference contrast microscopy evidence used in automatic assertion +def: "A type of differential interference contrast microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007055 ! differential interference contrast microscopy evidence +is_a: ECO:0007435 ! microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007615 +name: extended field laser confocal microscopy evidence used in automatic assertion +def: "A type of extended field laser confocal microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007057 ! extended field laser confocal microscopy evidence +is_a: ECO:0007490 ! confocal microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007616 +name: confocal laser scanning microscopy evidence used in automatic assertion +def: "A type of confocal laser scanning microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007059 ! confocal laser scanning microscopy evidence +is_a: ECO:0007490 ! confocal microscopy evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007617 +name: light scattering evidence used in automatic assertion +def: "A type of light scattering evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007061 ! light scattering evidence +is_a: ECO:0007815 ! structure determination evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007618 +name: dynamic light scattering assay evidence used in automatic assertion +def: "A type of dynamic light scattering assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007063 ! dynamic light scattering assay evidence +is_a: ECO:0007617 ! light scattering evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007619 +name: static light scattering assay evidence used in automatic assertion +def: "A type of static light scattering assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007065 ! static light scattering assay evidence +is_a: ECO:0007617 ! light scattering evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007620 +name: colony papillation assay phenotypic evidence used in automatic assertion +def: "A type of colony papillation assay phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "colony papillation assay evidence used in automatic assertion" EXACT [] +is_a: ECO:0007067 ! colony papillation assay phenotypic evidence +is_a: ECO:0007625 ! colony morphology phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007621 +name: crystal violet staining evidence used in automatic assertion +def: "A type of crystal violet staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007069 ! crystal violet staining evidence +is_a: ECO:0007798 ! staining evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007622 +name: flow cell biofilm assay evidence used in automatic assertion +def: "A type of flow cell biofilm assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007071 ! flow cell biofilm assay evidence +is_a: ECO:0007541 ! biofilm formation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007623 +name: bacterial 2-hybrid assay evidence used in automatic assertion +def: "A type of bacterial 2-hybrid assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007073 ! bacterial 2-hybrid assay evidence +is_a: ECO:0007432 ! bait-prey hybrid interaction evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007624 +name: phenomic profiling assay evidence used in automatic assertion +def: "A type of phenomic profiling assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007075 ! phenomic profiling assay evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007625 +name: colony morphology phenotypic evidence used in automatic assertion +def: "A type of colony morphology phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "colony morphology evidence used in automatic assertion" EXACT [] +is_a: ECO:0007077 ! colony morphology phenotypic evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007626 +name: colony color phenotypic evidence used in automatic assertion +def: "A type of colony color phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "colony color evidence used in automatic assertion" EXACT [] +is_a: ECO:0007079 ! colony color phenotypic evidence +is_a: ECO:0007625 ! colony morphology phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007627 +name: colony size phenotypic evidence used in automatic assertion +def: "A type of colony size phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "colony size evidence used in automatic assertion" EXACT [] +is_a: ECO:0007081 ! colony size phenotypic evidence +is_a: ECO:0007625 ! colony morphology phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007628 +name: zone of inhibition evidence used in automatic assertion +def: "A type of zone of inhibition evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007083 ! zone of inhibition evidence +is_a: ECO:0007778 ! cell growth assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007629 +name: Etest evidence used in automatic assertion +def: "A type of Etest evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007085 ! Etest evidence +is_a: ECO:0007628 ! zone of inhibition evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007630 +name: ribosome profiling evidence used in automatic assertion +def: "A type of ribosome profiling evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007087 ! ribosome profiling evidence +is_a: ECO:0007298 ! expression pattern evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007631 +name: computational inference used in manual assertion +def: "A type of evidence based on computational logical inference that is used in a manual assertion." [ECO:RCT] +synonym: "evidence based on computational logical inference used in manual assertion" EXACT [] +is_a: ECO:0000362 ! computational inference +is_a: ECO:0007668 ! computational evidence used in manual assertion +is_a: ECO:0007751 ! inferential evidence used in manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007632 +name: transcriptional activation assay evidence used in manual assertion +def: "A type of transcriptional activation assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000170 ! transcriptional activation assay evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-18T08:34:35Z + +[Term] +id: ECO:0007633 +name: transcriptional activation assay evidence used in automatic assertion +def: "A type of transcriptional activation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000170 ! transcriptional activation assay evidence +is_a: ECO:0007768 ! transcription assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-05-18T08:34:35Z + +[Term] +id: ECO:0007634 +name: experimental phenotypic evidence used in manual assertion +def: "A type of experimental phenotypic evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000059 ! experimental phenotypic evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-06-20T02:12:00Z + +[Term] +id: ECO:0007635 +name: experimental phenotypic evidence used in automatic assertion +def: "A type of experimental phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000059 ! experimental phenotypic evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2018-06-20T02:12:00Z + +[Term] +id: ECO:0007636 +name: curator inference from database +def: "A type of curator inference from authoritative resource based on information located in a queryable database and is optimized for computers." [ECO:RCT] +is_a: ECO:0007647 ! curator inference from authoritative source + +[Term] +id: ECO:0007637 +name: curator inference from encyclopedia +def: "A type of curator inference from published work where the reference is to an entry in a compendium that provides summarized information on a subject." [url:https\://en.wikipedia.org/wiki/Encyclopedia] +is_a: ECO:0007647 ! curator inference from authoritative source + +[Term] +id: ECO:0007638 +name: curator inference from Wikipedia +def: "A type of curator inference from encyclopedia where the reference is to a Wikipedia article." [ECO:RCT] +is_a: ECO:0007637 ! curator inference from encyclopedia + +[Term] +id: ECO:0007639 +name: curator inference from Britannica +def: "A type of curator inference from encyclopedia where the reference is to an Encyclopedia Britannica article." [ECO:RCT] +is_a: ECO:0007637 ! curator inference from encyclopedia + +[Term] +id: ECO:0007640 +name: curator inference from MedlinePlus encyclopedia +def: "A type of curator inference from encyclopedia in which the reference is to an article in the National Library of Medicine's MedLinePlus encyclopedia." [ECO:RCT] +is_a: ECO:0007637 ! curator inference from encyclopedia + +[Term] +id: ECO:0007641 +name: curator inference from dictionary +def: "A type of curator inference from published work in which the entry comes from a collection of words with definitions, usages, pronounciations, and more." [url:https\://en.wikipedia.org/wiki/Dictionary] +is_a: ECO:0007647 ! curator inference from authoritative source + +[Term] +id: ECO:0007642 +name: curator inference from Oxford Dictionary +def: "A type of curator inference from dictionary in which the reference is to an entry in the Oxford Dictionaries." [ECO:RCT] +is_a: ECO:0007641 ! curator inference from dictionary + +[Term] +id: ECO:0007643 +name: curator inference from Merriam-Webster Dictionary +def: "A type of curator inference from dictionary in which the reference is to an entry in the Merriam-Webster Dictionary." [ECO:RCT] +is_a: ECO:0007641 ! curator inference from dictionary + +[Term] +id: ECO:0007644 +name: curator inference from MedlinePlus dictionary +def: "A type of curator inference from dictionary in which the reference is to an entry in the National Library of Medicine's MedLinePlus dictionary." [ECO:RCT] +is_a: ECO:0007641 ! curator inference from dictionary + +[Term] +id: ECO:0007645 +name: curator inference from journal publication +def: "A type of curator inference from published work reporting on research findings." [url:https\://en.wikipedia.org/wiki/Scientific_journal] +is_a: ECO:0007647 ! curator inference from authoritative source + +[Term] +id: ECO:0007646 +name: curator inference from book +def: "A type of curator inference from published work based on a book, which may be a reference to a URL (for ebooks) or a DOI." [ECO:RCT] +is_a: ECO:0007647 ! curator inference from authoritative source + +[Term] +id: ECO:0007647 +name: curator inference from authoritative source +def: "A type of curator inference that is from what is generally considered an authoritative source on the topic, including model organism databases, newspaper articles, books, journal publications, etc." [ECO:RCT] +is_a: ECO:0000205 ! curator inference + +[Term] +id: ECO:0007648 +name: manually integrated combinatorial computational evidence +def: "A type of manually integrated combinatorial evidence in which two or more distinct types of computational evidence are integrated manually." [ECO:RCT] +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007649 +name: manually integrated combinatorial computational evidence used in manual assertion +def: "A type of manually integrated combinatorial computational evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0007648 ! manually integrated combinatorial computational evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007650 +name: manually integrated combinatorial computational evidence used in automatic assertion +def: "A type of manually integrated combinatorial computational evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000501 ! evidence used in automatic assertion +is_a: ECO:0007648 ! manually integrated combinatorial computational evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007651 +name: automatically integrated combinatorial computational evidence +def: "A type of automatically integrated combinatorial evidence in which two or more distinct types of computational evidence are integrated automatically." [ECO:RCT] +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007652 +name: automatically integrated combinatorial computational evidence used in manual assertion +def: "A type of automatically integrated combinatorial computational evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0007651 ! automatically integrated combinatorial computational evidence +property_value: ECO:9000002 "RCA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007653 +name: automatically integrated combinatorial computational evidence used in automatic assertion +def: "A type of automatically integrated combinatorial computational evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000501 ! evidence used in automatic assertion +is_a: ECO:0007651 ! automatically integrated combinatorial computational evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007654 +name: combinatorial experimental evidence +def: "A type of combinatorial evidence in which two or more distinct types of experimental evidence are integrated." [ECO:RCT] +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007655 +name: manually integrated combinatorial experimental evidence +def: "A type of manually integrated combinatorial evidence in which two or more distinct types of experimental evidence are integrated manually." [ECO:RCT] +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007656 +name: manually integrated combinatorial experimental evidence used in manual assertion +def: "A type of manually integrated combinatorial experimental evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0007655 ! manually integrated combinatorial experimental evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007657 +name: manually integrated combinatorial experimental evidence used in automatic assertion +def: "A type of manually integrated combinatorial experimental evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000501 ! evidence used in automatic assertion +is_a: ECO:0007655 ! manually integrated combinatorial experimental evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007658 +name: automatically integrated combinatorial experimental evidence +def: "A type of automatically integrated combinatorial evidence in which two or more distinct types of experimental evidence are integrated automatically." [ECO:RCT] +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007659 +name: automatically integrated combinatorial experimental evidence used in manual assertion +def: "A type of automatically integrated combinatorial experimental evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0007658 ! automatically integrated combinatorial experimental evidence +property_value: ECO:9000002 "RCA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007660 +name: automatically integrated combinatorial experimental evidence used in automatic assertion +def: "A type of automatically integrated combinatorial experimental evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000501 ! evidence used in automatic assertion +is_a: ECO:0007658 ! automatically integrated combinatorial experimental evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007661 +name: combinatorial computational and experimental evidence +def: "A type of combinatorial evidence in which at least one line of experimental evidence and at least one line of computational evidence have been integrated." [ECO:RCT] +is_a: ECO:0000212 ! combinatorial evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007662 +name: manually integrated combinatorial computational and experimental evidence +def: "A type of manually integrated combinatorial evidence in which at least one line of experimental evidence and at least one line of computational evidence have been integrated manually." [ECO:RCT] +is_a: ECO:0007661 ! combinatorial computational and experimental evidence +is_a: ECO:0007674 ! manually integrated combinatorial evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007663 +name: manually integrated combinatorial computational and experimental evidence used in manual assertion +def: "A type of manually integrated combinatorial computational and experimental evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0007662 ! manually integrated combinatorial computational and experimental evidence +is_a: ECO:0007675 ! manually integrated combinatorial evidence used in manual assertion +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007664 +name: manually integrated combinatorial computational and experimental evidence used in automatic assertion +def: "A type of manually integrated combinatorial computational and experimental evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007662 ! manually integrated combinatorial computational and experimental evidence +is_a: ECO:0007676 ! manually integrated combinatorial evidence used in automatic assertion +is_a: ECO:0007829 ! combinatorial computational and experimental evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007665 +name: automatically integrated combinatorial computational and experimental evidence +def: "A type of automatically integrated combinatorial evidence in which at least one line of experimental evidence and at least one line of computational evidence have been integrated automatically." [ECO:RCT] +is_a: ECO:0007661 ! combinatorial computational and experimental evidence +is_a: ECO:0007673 ! automatically integrated combinatorial evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007666 +name: automatically integrated combinatorial computational and experimental evidence used in manual assertion +def: "A type of automatically integrated combinatorial computational and experimental evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000245 ! automatically integrated combinatorial evidence used in manual assertion +is_a: ECO:0007665 ! automatically integrated combinatorial computational and experimental evidence +property_value: ECO:9000002 "RCA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007667 +name: automatically integrated combinatorial computational and experimental evidence used in automatic assertion +def: "A type of automatically integrated combinatorial computational and experimental evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000053 ! automatically integrated combinatorial evidence used in automatic assertion +is_a: ECO:0007665 ! automatically integrated combinatorial computational and experimental evidence +is_a: ECO:0007829 ! combinatorial computational and experimental evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007668 +name: computational evidence used in manual assertion +def: "A type of computational evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0007672 ! computational evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007669 +name: computational evidence used in automatic assertion +def: "A type of computational evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "IEA" EXACT [] +synonym: "inferred from electronic annotation" EXACT [GOECO:IEA] +xref: GO_REF:0000003 "Gene Ontology annotation based on Enzyme Commission mapping." +xref: GO_REF:0000004 "Gene Ontology annotation based on Swiss-Prot keyword mapping." +xref: GO_REF:0000020 "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins." +xref: GO_REF:0000041 "IEA (UniProt UniPathway)" +xref: GO_REF:0000043 "Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping." +xref: GO_REF:0000044 "Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt." +xref: GO_REF:0000116 "Automatic Gene Ontology annotation based on Rhea mapping." +xref: GOECO:IEA "inferred from electronic annotation" +is_a: ECO:0000501 ! evidence used in automatic assertion +is_a: ECO:0007672 ! computational evidence +property_value: ECO:9000002 "IEA" xsd:string {comment="Default"} +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007672 +name: computational evidence +def: "A type of evidence in which data are produced, and/or generated, and/or analyzed on a computer." [ECO:RCT] +synonym: "in silico evidence" EXACT [] +is_a: ECO:0000000 ! evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007673 +name: automatically integrated combinatorial evidence +def: "A type of combinatorial evidence in which at least two distinct types of evidence have been integrated automatically." [ECO:RCT] +is_a: ECO:0000212 ! combinatorial evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007674 +name: manually integrated combinatorial evidence +def: "A type of combinatorial evidence in which at least two distinct types of evidence have been integrated manually." [ECO:RCT] +is_a: ECO:0000212 ! combinatorial evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007675 +name: manually integrated combinatorial evidence used in manual assertion +def: "A type of manually integrated combinatorial evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000244 ! combinatorial evidence used in manual assertion +is_a: ECO:0007674 ! manually integrated combinatorial evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007676 +name: manually integrated combinatorial evidence used in automatic assertion +def: "A type of manually integrated combinatorial evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000213 ! combinatorial evidence used in automatic assertion +is_a: ECO:0007674 ! manually integrated combinatorial evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007677 +name: combinatorial computational evidence +def: "A type of combinatorial evidence in which at least two distinct types of computational evidence have been integrated." [ECO:RCT] +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007678 +name: combinatorial computational evidence used in manual assertion +def: "A type of combinatorial computational evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0007677 ! combinatorial computational evidence + +[Term] +id: ECO:0007679 +name: combinatorial computational evidence used in automatic assertion +def: "A type of combinatorial computational evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000501 ! evidence used in automatic assertion +is_a: ECO:0007677 ! combinatorial computational evidence +property_value: ECO:9000002 "IEA" xsd:string + +[Term] +id: ECO:0007680 +name: chromatography evidence used in manual assertion +def: "A type of chromatography evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0000325 ! chromatography evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-01-21T06:28:00Z + +[Term] +id: ECO:0007681 +name: chromatography evidence used in automatic assertion +def: "A type of chromatography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000325 ! chromatography evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-01-21T06:28:00Z + +[Term] +id: ECO:0007682 +name: reporter gene assay evidence used in manual assertion +def: "A type of reporter gene assay evidence that is used in a manual assertion." [] +is_a: ECO:0000049 ! reporter gene assay evidence +is_a: ECO:0000270 ! expression pattern evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007684 +name: protein separation evidence used in manual assertion +def: "A type of protein separation evidence that is used in a manual assertion." [] +is_a: ECO:0000156 ! protein separation evidence +is_a: ECO:0006005 ! fractionation evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007685 +name: substance quantification evidence used in manual assertion +def: "A type of substance quantification evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0000335 ! substance quantification evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007686 +name: voltage clamp recording evidence used in manual assertion +def: "A type of voltage clamp recording evidence that is used in a manual assertion." [] +is_a: ECO:0005576 ! voltage clamp recording evidence +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007687 +name: protein kinase assay evidence used in manual assertion +def: "A type of protein kinase assay evidence that is used in a manual assertion." [] +is_a: ECO:0001558 ! protein kinase assay evidence +is_a: ECO:0005801 ! enzymatic activity assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007688 +name: gel electrophoresis evidence used in manual assertion +def: "A type of gel electrophoresis evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0000337 ! gel electrophoresis evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007689 +name: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence used in manual assertion +def: "A type of sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007690 +name: ex vivo assay evidence used in manual assertion +def: "A type of ex vivo assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005502 ! ex vivo assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007691 +name: cleavage assay evidence used in manual assertion +def: "A type of cleavage assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001547 ! cleavage assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007692 +name: deacetylation assay evidence used in manual assertion +def: "A type of deacetylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001548 ! deacetylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007693 +name: transcription assay evidence used in manual assertion +def: "A type of transcription assay evidence that is used in a manual assertion." [] +is_a: ECO:0000168 ! transcription assay evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007694 +name: phosphatase assay evidence used in manual assertion +def: "A type of phosphatase assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001556 ! phosphatase assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007695 +name: cell-based assay evidence used in manual assertion +def: "A type of cell-based assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001565 ! cell-based assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007696 +name: cell proliferation assay evidence used in manual assertion +def: "A type of cell proliferation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005007 ! cell proliferation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007697 +name: DNA synthesis cell proliferation assay evidence used in manual assertion +def: "A type of DNA synthesis cell proliferation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005008 ! DNA synthesis cell proliferation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007698 +name: apoptotic assay evidence used in manual assertion +def: "A type of apoptotic assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007699 +name: cell growth assay evidence used in manual assertion +def: "A type of cell growth assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001563 ! cell growth assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007700 +name: disk diffusion test evidence used in manual assertion +def: "A type of disk diffusion test evidence that is used in a manual assertion." [] +is_a: ECO:0005605 ! disk diffusion test evidence +is_a: ECO:0007084 ! zone of inhibition evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007701 +name: chemotaxis assay evidence used in manual assertion +def: "A type of chemotaxis assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005021 ! chemotaxis assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007702 +name: cytotoxicity assay evidence used in manual assertion +def: "A type of cytotoxicity assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005012 ! cytotoxicity assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007703 +name: cell viability assay evidence used in manual assertion +def: "A type of cell viability assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005004 ! cell viability assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007704 +name: methylation assay evidence used in manual assertion +def: "A type of methylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001554 ! methylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007705 +name: protein assay evidence used in manual assertion +def: "A type of protein assay evidence that is used in a manual assertion." [] +is_obsolete: true +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007706 +name: chromatin immunoprecipitation evidence used in manual assertion +def: "A type of chromatin immunoprecipitation evidence that is used in a manual assertion." [] +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +is_a: ECO:0005644 ! immunoprecipitation evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007707 +name: protein inhibition evidence used in manual assertion +def: "A type of protein inhibition evidence that is used in a manual assertion." [] +is_a: ECO:0000020 ! protein inhibition evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007708 +name: sumoylation assay evidence used in manual assertion +def: "A type of sumoylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001559 ! sumoylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007709 +name: transport assay evidence used in manual assertion +def: "A type of transport assay evidence that is used in a manual assertion." [] +is_a: ECO:0000134 ! transport assay evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007710 +name: defarnesylation assay evidence used in manual assertion +def: "A type of defarnesylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001549 ! defarnesylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007711 +name: deubiquitination assay evidence used in manual assertion +def: "A type of deubiquitination assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001552 ! deubiquitination assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007712 +name: palmitoylation assay evidence used in manual assertion +def: "A type of palmitoylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001555 ! palmitoylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007713 +name: acetylation assay evidence used in manual assertion +def: "A type of acetylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001546 ! acetylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007714 +name: demethylation assay evidence used in manual assertion +def: "A type of demethylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001550 ! demethylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007715 +name: polyADP-ribosylation assay evidence used in manual assertion +def: "A type of polyADP-ribosylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001557 ! polyADP-ribosylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007716 +name: staining evidence used in manual assertion +def: "A type of staining evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005019 ! staining evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007717 +name: translation assay evidence used in manual assertion +def: "A type of translation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001561 ! translation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007718 +name: ubiquitination assay evidence used in manual assertion +def: "A type of ubiquitination assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001562 ! ubiquitination assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007719 +name: immunodetection assay evidence used in manual assertion +def: "A type of immunodetection assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0005593 ! immunodetection assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007720 +name: desumoylation assay evidence used in manual assertion +def: "A type of desumoylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001551 ! desumoylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007721 +name: farnesylation assay evidence used in manual assertion +def: "A type of farnesylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001553 ! farnesylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007722 +name: reconstitution assay evidence used in manual assertion +def: "A type of reconstitution assay evidence that is used in a manual assertion." [] +is_a: ECO:0000003 ! reconstitution assay evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007723 +name: in vitro transcription reconstitution assay evidence used in manual assertion +def: "A type of in vitro transcription reconstitution assay evidence that is used in a manual assertion." [] +is_a: ECO:0000150 ! in vitro transcription reconstitution assay evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007724 +name: localization evidence used in manual assertion +def: "A type of localization evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001533 ! localization evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007725 +name: protein localization evidence used in manual assertion +def: "A type of protein localization evidence that is used in a manual assertion." [] +is_a: ECO:0000122 ! protein localization evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007726 +name: fusion protein localization evidence used in manual assertion +def: "A type of fusion protein localization evidence that is used in a manual assertion." [] +is_a: ECO:0000124 ! fusion protein localization evidence +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007727 +name: nucleic acid localization evidence used in manual assertion +def: "A type of nucleic acid localization evidence that is used in a manual assertion." [] +is_a: ECO:0000314 ! direct assay evidence used in manual assertion +is_a: ECO:0001534 ! nucleic acid localization evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007728 +name: anatomical perturbation phenotypic evidence used in manual assertion +def: "A type of anatomical perturbation phenotypic evidence that is used in a manual assertion." [] +is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007730 +name: cleavage arrested development evidence used in manual assertion +def: "A type of cleavage arrested development evidence that is used in a manual assertion." [] +is_a: ECO:0000298 ! cleavage arrested development evidence +is_a: ECO:0007634 ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007731 +name: spectrometry evidence used in manual assertion +def: "A type of spectrometry evidence that is used in a manual assertion." [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0005504 ! spectrometry evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007732 +name: sequencing assay evidence used in manual assertion +def: "A type of sequencing assay evidence that is used in a manual assertion." [] +is_a: ECO:0000220 ! sequencing assay evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007733 +name: nucleotide sequencing assay evidence used in manual assertion +def: "A type of nucleotide sequencing assay evidence that is used in a manual assertion." [] +is_a: ECO:0000219 ! nucleotide sequencing assay evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007734 +name: high throughput nucleotide sequencing assay evidence used in manual assertion +def: "A type of high throughput nucleotide sequencing assay evidence that is used in a manual assertion." [] +is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007735 +name: structure determination evidence used in manual assertion +def: "A type of structure determination evidence that is used in a manual assertion." [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0005031 ! structure determination evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007736 +name: molecule detection assay evidence used in manual assertion +def: "A type of molecule detection assay evidence that is used in a manual assertion." [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0005516 ! molecule detection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007737 +name: DNA detection assay evidence used in manual assertion +def: "A type of DNA detection assay evidence that is used in a manual assertion." [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0005519 ! DNA detection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007738 +name: protein detection assay evidence used in manual assertion +def: "A type of protein detection assay evidence that is used in a manual assertion." [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0005517 ! protein detection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007739 +name: RNA detection assay evidence used in manual assertion +def: "A type of RNA detection assay evidence that is used in a manual assertion." [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0005518 ! RNA detection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007740 +name: small molecule detection assay evidence used in manual assertion +def: "A type of small molecule detection assay evidence that is used in a manual assertion." [] +is_a: ECO:0000269 ! experimental evidence used in manual assertion +is_a: ECO:0001522 ! small molecule detection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007741 +name: chromosome conformation-based evidence used in manual assertion +def: "A type of chromosome conformation-based evidence that is used in a manual assertion." [] +is_a: ECO:0000232 ! chromosome conformation-based evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007742 +name: 3C evidence used in manual assertion +def: "A type of 3C evidence that is used in a manual assertion." [] +is_a: ECO:0000233 ! 3C evidence +is_a: ECO:0000269 ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007744 +name: combinatorial computational and experimental evidence used in manual assertion +def: "A type of combinatorial computational and experimental evidence that is used in a manual assertion." [] +is_a: ECO:0000244 ! combinatorial evidence used in manual assertion +is_a: ECO:0007661 ! combinatorial computational and experimental evidence +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007745 +name: combinatorial experimental evidence used in manual assertion +def: "A type of combinatorial experimental evidence that is used in a manual assertion." [] +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0007654 ! combinatorial experimental evidence +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007746 +name: biological system reconstruction evidence used in manual assertion +def: "A type of biological system reconstruction evidence that is used in a manual assertion." [] +is_a: ECO:0000088 ! biological system reconstruction evidence +is_a: ECO:0000352 ! evidence used in manual assertion +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007747 +name: biological system reconstruction evidence by experimental evidence used in manual assertion +def: "A type of biological system reconstruction evidence by experimental evidence that is used in a manual assertion." [] +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0005551 ! biological system reconstruction evidence by experimental evidence +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007748 +name: phenotypic similarity evidence used in manual assertion +def: "A type of phenotypic similarity evidence that is used in a manual assertion." [] +is_a: ECO:0000057 ! phenotypic similarity evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007749 +name: transcript splice pattern evidence used in manual assertion +def: "A type of transcript splice pattern evidence that is used in a manual assertion." [] +is_a: ECO:0000247 ! sequence alignment evidence used in manual assertion +is_a: ECO:0000326 ! transcript splice pattern evidence +property_value: ECO:9000002 "ISA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007750 +name: phylogenetic evidence used in manual assertion +def: "A type of phylogenetic evidence that is used in a manual assertion." [] +is_a: ECO:0000080 ! phylogenetic evidence +is_a: ECO:0000252 ! similarity evidence used in manual assertion +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007751 +name: inferential evidence used in manual assertion +def: "A type of inferential evidence that is used in a manual assertion." [] +is_a: ECO:0000352 ! evidence used in manual assertion +is_a: ECO:0000361 ! inferential evidence +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007752 +name: curator inference from authoritative source used in manual assertion +def: "A type of curator inference from authoritative source that is used in a manual assertion." [] +is_a: ECO:0000305 ! curator inference used in manual assertion +is_a: ECO:0007647 ! curator inference from authoritative source +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007753 +name: curator inference from encyclopedia used in manual assertion +def: "A type of curator inference from encyclopedia that is used in a manual assertion." [] +is_a: ECO:0007637 ! curator inference from encyclopedia +is_a: ECO:0007752 ! curator inference from authoritative source used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007754 +name: curator inference from Wikipedia used in manual assertion +def: "A type of curator inference from Wikipedia that is used in a manual assertion." [] +is_a: ECO:0007638 ! curator inference from Wikipedia +is_a: ECO:0007753 ! curator inference from encyclopedia used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007755 +name: curator inference from MedlinePlus encyclopedia used in manual assertion +def: "A type of curator inference from MedlinePlus encyclopedia that is used in a manual assertion." [] +is_a: ECO:0007640 ! curator inference from MedlinePlus encyclopedia +is_a: ECO:0007753 ! curator inference from encyclopedia used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007756 +name: curator inference from Britannica used in manual assertion +def: "A type of curator inference from Britannica that is used in a manual assertion." [] +is_a: ECO:0007639 ! curator inference from Britannica +is_a: ECO:0007753 ! curator inference from encyclopedia used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007757 +name: curator inference from book used in manual assertion +def: "A type of curator inference from book that is used in a manual assertion." [] +is_a: ECO:0007646 ! curator inference from book +is_a: ECO:0007752 ! curator inference from authoritative source used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007758 +name: curator inference from journal publication used in manual assertion +def: "A type of curator inference from journal publication that is used in a manual assertion." [] +is_a: ECO:0007645 ! curator inference from journal publication +is_a: ECO:0007752 ! curator inference from authoritative source used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007759 +name: curator inference from database used in manual assertion +def: "A type of curator inference from database that is used in a manual assertion." [] +is_a: ECO:0007636 ! curator inference from database +is_a: ECO:0007752 ! curator inference from authoritative source used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007760 +name: curator inference from dictionary used in manual assertion +def: "A type of curator inference from dictionary that is used in a manual assertion." [] +is_a: ECO:0007641 ! curator inference from dictionary +is_a: ECO:0007752 ! curator inference from authoritative source used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007761 +name: curator inference from MedlinePlus dictionary used in manual assertion +def: "A type of curator inference from MedlinePlus dictionary that is used in a manual assertion." [] +is_a: ECO:0007644 ! curator inference from MedlinePlus dictionary +is_a: ECO:0007760 ! curator inference from dictionary used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007762 +name: curator inference from Oxford Dictionary used in manual assertion +def: "A type of curator inference from Oxford Dictionary that is used in a manual assertion." [] +is_a: ECO:0007642 ! curator inference from Oxford Dictionary +is_a: ECO:0007760 ! curator inference from dictionary used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007763 +name: curator inference from Merriam-Webster Dictionary used in manual assertion +def: "A type of curator inference from Merriam-Webster Dictionary that is used in a manual assertion." [] +is_a: ECO:0007643 ! curator inference from Merriam-Webster Dictionary +is_a: ECO:0007760 ! curator inference from dictionary used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007765 +name: reporter gene assay evidence used in automatic assertion +def: "A type of reporter gene assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000049 ! reporter gene assay evidence +is_a: ECO:0007298 ! expression pattern evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007766 +name: voltage clamp recording evidence used in automatic assertion +def: "A type of voltage clamp recording evidence that is used in an automatic assertion." [] +is_a: ECO:0005576 ! voltage clamp recording evidence +is_a: ECO:0007535 ! electrophysiology assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007767 +name: protein kinase assay evidence used in automatic assertion +def: "A type of protein kinase assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001558 ! protein kinase assay evidence +is_a: ECO:0007468 ! enzymatic activity assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007768 +name: transcription assay evidence used in automatic assertion +def: "A type of transcription assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000168 ! transcription assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007769 +name: gel electrophoresis evidence used in automatic assertion +def: "A type of gel electrophoresis evidence that is used in an automatic assertion." [] +is_a: ECO:0000337 ! gel electrophoresis evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007770 +name: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence used in automatic assertion +def: "A type of sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence that is used in an automatic assertion." [] +is_a: ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence +is_a: ECO:0007769 ! gel electrophoresis evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007771 +name: deacetylation assay evidence used in automatic assertion +def: "A type of deacetylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001548 ! deacetylation assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007772 +name: phosphatase assay evidence used in automatic assertion +def: "A type of phosphatase assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001556 ! phosphatase assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007773 +name: cell-based assay evidence used in automatic assertion +def: "A type of cell-based assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001565 ! cell-based assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007774 +name: cell viability assay evidence used in automatic assertion +def: "A type of cell viability assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005004 ! cell viability assay evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007775 +name: cell proliferation assay evidence used in automatic assertion +def: "A type of cell proliferation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005007 ! cell proliferation assay evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007776 +name: DNA synthesis cell proliferation assay evidence used in automatic assertion +def: "A type of DNA synthesis cell proliferation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005008 ! DNA synthesis cell proliferation assay evidence +is_a: ECO:0007775 ! cell proliferation assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007777 +name: apoptotic assay evidence used in automatic assertion +def: "A type of apoptotic assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005034 ! apoptotic assay evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007778 +name: cell growth assay evidence used in automatic assertion +def: "A type of cell growth assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001563 ! cell growth assay evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007779 +name: disk diffusion test evidence used in automatic assertion +def: "A type of disk diffusion test evidence that is used in an automatic assertion." [] +is_a: ECO:0005605 ! disk diffusion test evidence +is_a: ECO:0007628 ! zone of inhibition evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007780 +name: chemotaxis assay evidence used in automatic assertion +def: "A type of chemotaxis assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005021 ! chemotaxis assay evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007781 +name: cytotoxicity assay evidence used in automatic assertion +def: "A type of cytotoxicity assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005012 ! cytotoxicity assay evidence +is_a: ECO:0007773 ! cell-based assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007782 +name: cleavage assay evidence used in automatic assertion +def: "A type of cleavage assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001547 ! cleavage assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007783 +name: methylation assay evidence used in automatic assertion +def: "A type of methylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001554 ! methylation assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007784 +name: protein assay evidence used in automatic assertion +def: "A type of protein assay evidence that is used in an automatic assertion." [] +is_obsolete: true +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007785 +name: protein inhibition evidence used in automatic assertion +def: "A type of protein inhibition evidence that is used in an automatic assertion." [] +is_a: ECO:0000020 ! protein inhibition evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007786 +name: chromatin immunoprecipitation evidence used in automatic assertion +def: "A type of chromatin immunoprecipitation evidence that is used in an automatic assertion." [] +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +is_a: ECO:0007503 ! immunoprecipitation evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007787 +name: protein separation evidence used in automatic assertion +def: "A type of protein separation evidence that is used in an automatic assertion." [] +is_a: ECO:0000156 ! protein separation evidence +is_a: ECO:0007534 ! fractionation evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007788 +name: sumoylation assay evidence used in automatic assertion +def: "A type of sumoylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001559 ! sumoylation assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007789 +name: transport assay evidence used in automatic assertion +def: "A type of transport assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000134 ! transport assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007790 +name: defarnesylation assay evidence used in automatic assertion +def: "A type of defarnesylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001549 ! defarnesylation assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007791 +name: deubiquitination assay evidence used in automatic assertion +def: "A type of deubiquitination assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001552 ! deubiquitination assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007792 +name: palmitoylation assay evidence used in automatic assertion +def: "A type of palmitoylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001555 ! palmitoylation assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007793 +name: ex vivo assay evidence used in automatic assertion +def: "A type of ex vivo assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005502 ! ex vivo assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007794 +name: acetylation assay evidence used in automatic assertion +def: "A type of acetylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001546 ! acetylation assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007795 +name: demethylation assay evidence used in automatic assertion +def: "A type of demethylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001550 ! demethylation assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007796 +name: immunodetection assay evidence used in automatic assertion +def: "A type of immunodetection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005593 ! immunodetection assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007797 +name: polyADP-ribosylation assay evidence used in automatic assertion +def: "A type of polyADP-ribosylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001557 ! polyADP-ribosylation assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007798 +name: staining evidence used in automatic assertion +def: "A type of staining evidence that is used in an automatic assertion." [] +is_a: ECO:0005019 ! staining evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007799 +name: translation assay evidence used in automatic assertion +def: "A type of translation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001561 ! translation assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007800 +name: farnesylation assay evidence used in automatic assertion +def: "A type of farnesylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001553 ! farnesylation assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007801 +name: ubiquitination assay evidence used in automatic assertion +def: "A type of ubiquitination assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001562 ! ubiquitination assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007802 +name: desumoylation assay evidence used in automatic assertion +def: "A type of desumoylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001551 ! desumoylation assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007803 +name: reconstitution assay evidence used in automatic assertion +def: "A type of reconstitution assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000003 ! reconstitution assay evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007804 +name: in vitro transcription reconstitution assay evidence used in automatic assertion +def: "A type of in vitro transcription reconstitution assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000150 ! in vitro transcription reconstitution assay evidence +is_a: ECO:0007803 ! reconstitution assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007805 +name: substance quantification evidence used in automatic assertion +def: "A type of substance quantification evidence that is used in an automatic assertion." [] +is_a: ECO:0000335 ! substance quantification evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007806 +name: localization evidence used in automatic assertion +def: "A type of localization evidence that is used in an automatic assertion." [] +is_a: ECO:0001533 ! localization evidence +is_a: ECO:0007307 ! direct assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007807 +name: protein localization evidence used in automatic assertion +def: "A type of protein localization evidence that is used in an automatic assertion." [] +is_a: ECO:0000122 ! protein localization evidence +is_a: ECO:0007806 ! localization evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007808 +name: fusion protein localization evidence used in automatic assertion +def: "A type of fusion protein localization evidence that is used in an automatic assertion." [] +is_a: ECO:0000124 ! fusion protein localization evidence +is_a: ECO:0007807 ! protein localization evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007809 +name: nucleic acid localization evidence used in automatic assertion +def: "A type of nucleic acid localization evidence that is used in an automatic assertion." [] +is_a: ECO:0001534 ! nucleic acid localization evidence +is_a: ECO:0007806 ! localization evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007810 +name: anatomical perturbation phenotypic evidence used in automatic assertion +def: "A type of anatomical perturbation phenotypic evidence that is used in an automatic assertion." [] +is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007811 +name: cleavage arrested development evidence used in automatic assertion +def: "A type of cleavage arrested development evidence that is used in an automatic assertion." [] +is_a: ECO:0000298 ! cleavage arrested development evidence +is_a: ECO:0007635 ! experimental phenotypic evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007812 +name: sequencing assay evidence used in automatic assertion +def: "A type of sequencing assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000220 ! sequencing assay evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007813 +name: nucleotide sequencing assay evidence used in automatic assertion +def: "A type of nucleotide sequencing assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000219 ! nucleotide sequencing assay evidence +is_a: ECO:0007812 ! sequencing assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007814 +name: high throughput nucleotide sequencing assay evidence used in automatic assertion +def: "A type of high throughput nucleotide sequencing assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence +is_a: ECO:0007813 ! nucleotide sequencing assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007815 +name: structure determination evidence used in automatic assertion +def: "A type of structure determination evidence that is used in an automatic assertion." [] +is_a: ECO:0005031 ! structure determination evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007816 +name: molecule detection assay evidence used in automatic assertion +def: "A type of molecule detection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005516 ! molecule detection assay evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007817 +name: DNA detection assay evidence used in automatic assertion +def: "A type of DNA detection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005519 ! DNA detection assay evidence +is_a: ECO:0007816 ! molecule detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007818 +name: RNA detection assay evidence used in automatic assertion +def: "A type of RNA detection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005518 ! RNA detection assay evidence +is_a: ECO:0007816 ! molecule detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007819 +name: protein detection assay evidence used in automatic assertion +def: "A type of protein detection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005517 ! protein detection assay evidence +is_a: ECO:0007816 ! molecule detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007820 +name: small molecule detection assay evidence used in automatic assertion +def: "A type of small molecule detection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001522 ! small molecule detection assay evidence +is_a: ECO:0007816 ! molecule detection assay evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007821 +name: spectrometry evidence used in automatic assertion +def: "A type of spectrometry evidence that is used in an automatic assertion." [] +is_a: ECO:0005504 ! spectrometry evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007822 +name: chromosome conformation-based evidence used in automatic assertion +def: "A type of chromosome conformation-based evidence that is used in an automatic assertion." [] +is_a: ECO:0000232 ! chromosome conformation-based evidence +is_a: ECO:0007297 ! experimental evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007823 +name: 3C evidence used in automatic assertion +def: "A type of 3C evidence that is used in an automatic assertion." [] +is_a: ECO:0000233 ! 3C evidence +is_a: ECO:0007822 ! chromosome conformation-based evidence used in automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007824 +name: phenotypic similarity evidence used in automatic assertion +def: "A type of phenotypic similarity evidence that is used in an automatic assertion." [] +is_a: ECO:0000057 ! phenotypic similarity evidence +is_a: ECO:0000251 ! similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007825 +name: transcript splice pattern evidence used in automatic assertion +def: "A type of transcript splice pattern evidence that is used in an automatic assertion." [] +is_a: ECO:0000248 ! sequence alignment evidence used in automatic assertion +is_a: ECO:0000326 ! transcript splice pattern evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007826 +name: phylogenetic evidence used in automatic assertion +def: "A type of phylogenetic evidence that is used in an automatic assertion." [] +is_a: ECO:0000080 ! phylogenetic evidence +is_a: ECO:0000251 ! similarity evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007827 +name: biological system reconstruction evidence used in automatic assertion +def: "A type of biological system reconstruction evidence that is used in an automatic assertion." [] +is_a: ECO:0000088 ! biological system reconstruction evidence +is_a: ECO:0000501 ! evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007828 +name: biological system reconstruction evidence by experimental evidence used in automatic assertion +def: "A type of biological system reconstruction evidence by experimental evidence that is used in an automatic assertion." [] +is_a: ECO:0005551 ! biological system reconstruction evidence by experimental evidence +is_a: ECO:0007827 ! biological system reconstruction evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007829 +name: combinatorial computational and experimental evidence used in automatic assertion +def: "A type of combinatorial computational and experimental evidence that is used in an automatic assertion." [] +is_a: ECO:0000213 ! combinatorial evidence used in automatic assertion +is_a: ECO:0007661 ! combinatorial computational and experimental evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007830 +name: combinatorial experimental evidence used in automatic assertion +def: "A type of combinatorial experimental evidence that is used in an automatic assertion." [] +is_a: ECO:0000501 ! evidence used in automatic assertion +is_a: ECO:0007654 ! combinatorial experimental evidence +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007832 +name: inferential evidence used in automatic assertion +def: "A type of inferential evidence that is used in an automatic assertion." [] +is_a: ECO:0000361 ! inferential evidence +is_a: ECO:0000501 ! evidence used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007833 +name: curator inference from authoritative source used in automatic assertion +def: "A type of curator inference from authoritative source that is used in an automatic assertion." [] +is_a: ECO:0007322 ! curator inference used in automatic assertion +is_a: ECO:0007647 ! curator inference from authoritative source +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007834 +name: curator inference from encyclopedia used in automatic assertion +def: "A type of curator inference from encyclopedia that is used in an automatic assertion." [] +is_a: ECO:0007637 ! curator inference from encyclopedia +is_a: ECO:0007833 ! curator inference from authoritative source used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007835 +name: curator inference from Wikipedia used in automatic assertion +def: "A type of curator inference from Wikipedia that is used in an automatic assertion." [] +is_a: ECO:0007638 ! curator inference from Wikipedia +is_a: ECO:0007834 ! curator inference from encyclopedia used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007836 +name: curator inference from Britannica used in automatic assertion +def: "A type of curator inference from Britannica that is used in an automatic assertion." [] +is_a: ECO:0007639 ! curator inference from Britannica +is_a: ECO:0007834 ! curator inference from encyclopedia used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007837 +name: curator inference from MedlinePlus encyclopedia used in automatic assertion +def: "A type of curator inference from MedlinePlus encyclopedia that is used in an automatic assertion." [] +is_a: ECO:0007640 ! curator inference from MedlinePlus encyclopedia +is_a: ECO:0007834 ! curator inference from encyclopedia used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007838 +name: curator inference from book used in automatic assertion +def: "A type of curator inference from book that is used in an automatic assertion." [] +is_a: ECO:0007646 ! curator inference from book +is_a: ECO:0007833 ! curator inference from authoritative source used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007839 +name: curator inference from dictionary used in automatic assertion +def: "A type of curator inference from dictionary that is used in an automatic assertion." [] +is_a: ECO:0007641 ! curator inference from dictionary +is_a: ECO:0007833 ! curator inference from authoritative source used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007840 +name: curator inference from MedlinePlus dictionary used in automatic assertion +def: "A type of curator inference from MedlinePlus dictionary that is used in an automatic assertion." [] +is_a: ECO:0007644 ! curator inference from MedlinePlus dictionary +is_a: ECO:0007839 ! curator inference from dictionary used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007841 +name: curator inference from Merriam-Webster Dictionary used in automatic assertion +def: "A type of curator inference from Merriam-Webster Dictionary that is used in an automatic assertion." [] +is_a: ECO:0007643 ! curator inference from Merriam-Webster Dictionary +is_a: ECO:0007839 ! curator inference from dictionary used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007842 +name: curator inference from Oxford Dictionary used in automatic assertion +def: "A type of curator inference from Oxford Dictionary that is used in an automatic assertion." [] +is_a: ECO:0007642 ! curator inference from Oxford Dictionary +is_a: ECO:0007839 ! curator inference from dictionary used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007843 +name: curator inference from journal publication used in automatic assertion +def: "A type of curator inference from journal publication that is used in an automatic assertion." [] +is_a: ECO:0007645 ! curator inference from journal publication +is_a: ECO:0007833 ! curator inference from authoritative source used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007844 +name: radioisotope quantification assay evidence +def: "A type of radioisotope assay evidence in which radioisotopic labeling is used to quantify a substance after a biochemical process or extraction from a biological sample." [] +is_a: ECO:0001120 ! radioisotope assay evidence +property_value: IAO:0000234 "dosumis" xsd:string +created_by: rcjackson +creation_date: 2019-06-12T10:46:00Z + +[Term] +id: ECO:0007845 +name: radioisotope quantification assay evidence used in automatic assertion +def: "A type of radioisotope quantification assay evidence that is used in an automatic assertion." [] +is_a: ECO:0007455 ! radioisotope assay evidence used in automatic assertion +is_a: ECO:0007844 ! radioisotope quantification assay evidence +created_by: rcjackson +creation_date: 2019-06-12T10:46:00Z + +[Term] +id: ECO:0007846 +name: radioisotope quantification assay evidence used in manual assertion +def: "A type of radioisotope quantification assay evidence that is used in a manual assertion." [] +is_a: ECO:0001254 ! radioisotope assay evidence used in manual assertion +is_a: ECO:0007844 ! radioisotope quantification assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rcjackson +creation_date: 2019-06-12T10:46:00Z + +[Term] +id: ECO:0007847 +name: fluorescence quantification assay evidence +def: "A type of fluorescence evidence in which fluorescent labeling is used to quantify a substance after a biochemical process or extraction from a biological sample." [] +is_a: ECO:0001115 ! fluorescence evidence +created_by: rcjackson +creation_date: 2019-06-12T10:46:00Z + +[Term] +id: ECO:0007848 +name: fluorescence quantification assay evidence used in automatic assertion +def: "A type of fluorescence quantification assay evidence that is used in an automatic assertion." [] +is_a: ECO:0007450 ! fluorescence evidence used in automatic assertion +is_a: ECO:0007847 ! fluorescence quantification assay evidence +created_by: rcjackson +creation_date: 2019-06-12T10:46:00Z + +[Term] +id: ECO:0007849 +name: fluorescence quantification assay evidence used in manual assertion +def: "A type of fluorescence quantification assay evidence that is used in a manual assertion." [] +is_a: ECO:0001249 ! fluorescence evidence used in manual assertion +is_a: ECO:0007847 ! fluorescence quantification assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rcjackson +creation_date: 2019-06-12T10:46:00Z + +[Term] +id: ECO:0007850 +name: inference of sequence features from visual inspection +def: "A type of inferential evidence in which sequence features are inferred based on visual inspection of the sequence." [ECO:RCJ] +comment: This type of inference may be made by either a curator or an author. +is_a: ECO:0000361 ! inferential evidence +created_by: beckyjackson +creation_date: 2019-11-22T06:51:00Z + +[Typedef] +id: used_in +name: used_in +def: "A relation connecting a piece of evidence to an assertion method, where that assertion method is supported by the evidence." [GOC:cjm] +comment: In the future we may use a more generic relation with weaker domain and range constraints taken from IAO, RO or OBI. +xref: ECO:9000000 +domain: ECO:0000000 ! evidence +range: ECO:0000217 ! assertion method +inverse_of: uses ! uses +created_by: mchibucos +creation_date: 2010-12-09T05:00:20Z + +[Typedef] +id: uses +name: uses +xref: ECO:9000001 + diff --git a/OWLTools-Annotation/src/test/resources/eco.20211012.obo b/OWLTools-Annotation/src/test/resources/eco.20211012.obo new file mode 100644 index 000000000..10b1c17aa --- /dev/null +++ b/OWLTools-Annotation/src/test/resources/eco.20211012.obo @@ -0,0 +1,21936 @@ +format-version: 1.2 +data-version: releases/2021-10-08 +date: 08:10:2021 13:56 +subsetdef: go_groupings "Grouping classes used by GO" +subsetdef: valid_with_biological_process "GO biological process terms should be used in the GO with/from field" +subsetdef: valid_with_cellular_component "GO cellular component terms should be used in the GO with/from field" +subsetdef: valid_with_chemical_entity "Chemical entity IDs should be used in the GO with/from field" +subsetdef: valid_with_gene "Gene IDs should be used in the GO with/from field" +subsetdef: valid_with_molecular_function "GO molecular function terms should be used in the GO with/from field" +subsetdef: valid_with_protein "Protein IDs should be used in the GO with/from field" +subsetdef: valid_with_protein_complex "Protein complex IDs should be used in the GO with/from field" +subsetdef: valid_with_transcript "Transcript IDs should be used in the GO with/from field" +default-namespace: eco +remark: ECO (https://github.com/evidenceontology/evidenceontology) is released into the public domain under CC0 1.0 Universal (CC0 1.0). Anyone is free to copy, modify, or distribute the work, even for commercial purposes, without asking permission. Please see the Public Domain Dedication (https://creativecommons.org/publicdomain/zero/1.0/) for an easy-to-read description of CC0 1.0 or the full legal code (https://creativecommons.org/publicdomain/zero/1.0/legalcode) for more detailed information. To get a sense of why ECO is CC0 as opposed to licensed under CC-BY, please read this thoughtful discussion (https://github.com/OBOFoundry/OBOFoundry.github.io/issues/285) on the OBO Foundry GitHub site. +ontology: eco +property_value: http://purl.org/dc/elements/1.1/description "The Evidence & Conclusion Ontology (ECO) describes types of scientific evidence within the biological research domain that arise from laboratory experiments, computational methods, literature curation, or other means." xsd:string +property_value: http://purl.org/dc/elements/1.1/title "Evidence & Conclusion Ontology (ECO)" xsd:string +property_value: http://purl.org/dc/terms/license https://creativecommons.org/publicdomain/zero/1.0/ + +[Term] +id: ECO:0000000 +name: evidence +def: "A type of information that is used to support an assertion." [ECO:MCC] +synonym: "evidence code" RELATED [] +synonym: "evidence_code" RELATED [] +disjoint_from: ECO:0000217 ! assertion method + +[Term] +id: ECO:0000001 +name: inference from background scientific knowledge +def: "A type of curator inference where conclusions are drawn based on the background scientific knowledge of the curator." [ECO:SN] +is_a: ECO:0000205 ! curator inference + +[Term] +id: ECO:0000002 +name: direct assay evidence +alt_id: ECO:0005006 +def: "A type of experimental evidence resulting from the direct measurement of some aspect of a biological feature." [ECO:SN] +is_a: ECO:0000006 ! experimental evidence + +[Term] +id: ECO:0000003 +name: reconstitution assay evidence +def: "A type of direct assay evidence based on reconstructing a biological sample from its disassociated state to its original state." [ECO:KAV, PMID:26029343] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000004 +name: cell fractionation evidence +def: "A type of fractionation evidence where sub-cellular components are separated based on their physical properties such as density in a sucrose density gradient." [ECO:KIM, TAIR:TED] +comment: If using this term for Gene Ontology annotation, it would be used most typically for annotations to the cellular component ontology. +synonym: "cell fractionation" RELATED [] +is_a: ECO:0000100 ! fractionation evidence + +[Term] +id: ECO:0000005 +name: enzymatic activity assay evidence +alt_id: ECO:0005001 +def: "A type of protein assay evidence where the catalytic activity of an enzyme is determined." [url:http\://www.sciencedirect.com/science/article/pii/S2213020914000068] +synonym: "enzyme assay evidence" EXACT [] +synonym: "enzyme assays" RELATED [] +xref: MI:0415 "enzymatic study" +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000006 +name: experimental evidence +alt_id: ECO:0005023 +def: "A type of evidence resulting from manipulation of variables in order to discover cause and effect." [url:http\://holah.co.uk/page/experimental/] +subset: go_groupings +is_a: ECO:0000000 ! evidence + +[Term] +id: ECO:0000007 +name: immunofluorescence evidence +def: "A type of protein detection assay evidence where a fluorescently labeled antibody is used to detect the presence or localization of a biomolecule within a cell." [ECO:MCC, TAIR:TED] +synonym: "immunofluorescence" RELATED [] +is_a: ECO:0005517 ! protein detection assay evidence + +[Term] +id: ECO:0000008 +name: expression pattern evidence +def: "A type of experimental evidence that is based on characterization of gene expression." [ECO:MCC, GO:IEP] +comment: Use this evidence type when the annotation is inferred from the timing or location of expression of a gene. It may be difficult to determine whether the expression pattern truly indicates that a gene plays a role in a given process. +is_a: ECO:0000006 ! experimental evidence +property_value: IAO:0000112 "The 10 previously identified GlnR-regulated genes were all confirmed to be under GlnR control during nitrogen stress (i.e. differential expression in the wild type compared to the DeltaglnR mutant), but in addition a total of 392 genes were significantly up-regulated and 291 significantly down regulated (Additional file 1: Table S1). This indicates that GlnR mediates (directly or indirectly) the expression of over 680 genes." xsd:string {xref="PMID:23642041"} + +[Term] +id: ECO:0000009 +name: transcript expression evidence +alt_id: ECO:0000048 +def: "A type of expression pattern evidence where abundance of a transcript is analyzed." [ECO:RCT] +synonym: "transcript expression level evidence" EXACT [] +synonym: "transcriptomics evidence" EXACT [] +is_a: ECO:0000008 ! expression pattern evidence + +[Term] +id: ECO:0000010 +name: protein expression evidence +alt_id: ECO:0000046 +def: "A type of expression pattern evidence resulting from protein abundance quantification techniques." [NBK:22011, PMC:4029002, url:http\://www.informatics.jax.org/glossary/gain-of-function, url:https\://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/overview-protein-expression-systems.html] +synonym: "protein expression level evidence" EXACT [] +is_a: ECO:0000008 ! expression pattern evidence +property_value: IAO:0000112 "In the BL21(DE3)(pJS429) cells, the levels of ClpP2s and VapC10 remained unchanged over a 2-hour period of translation arrest, but the VapB10 level showed to be decreased with a half-life (Figure 7C) similar to that observed in the strain BL21(DE3)(pJS883) (Figure 7B). These indicate that ClpXP2s could degrade VapB10 regardless of the presence or absence of VapC10." xsd:string {xref="PMID:24260461"} + +[Term] +id: ECO:0000011 +name: genetic interaction evidence +def: "A type of experimental phenotypic evidence resulting from the effect that a given gene has on another gene or genes, and the products." [ECO:RCT, PMID:11822023] +xref: TAIR:TED +is_a: ECO:0000059 ! experimental phenotypic evidence + +[Term] +id: ECO:0000012 +name: functional complementation evidence +def: "A type of genetic interaction evidence where a wild-type copy of the gene in question is inserted into a mutant cell to see if it restores the wild-type phenotype in the mutant background." [PMID:27403640] +synonym: "functional complementation" RELATED [] +is_a: ECO:0000011 ! genetic interaction evidence +property_value: IAO:0000112 "In addition, complementation of the ompR mutation in strain AR6 with plasmid pBR3 resulted in an increase in beta-galactosidase activity (1303 +- 80 Miller units), indicating that the His-tagged OmpR protein, expressed from the gene introduced in trans, was able to positively regulate flhDC expression." xsd:string {xref="PMID:20830609"} +property_value: IAO:0000112 "Trans-complementation of fimR on pDL276 (pHR6) in strain DeltafimR harboring pfim(445 b)-cat restored wild-type pfim expression (Figure S1). Taken together, pfim is negatively regulated by FimR." xsd:string {xref="PMID:23823757"} + +[Term] +id: ECO:0000013 +name: transgenic rescue experiment evidence +def: "A type of functional complementation evidence resulting from the introduction of a transgene to prevent, or \"rescue\" an organism from a condition." [url:http\://www.mdpi.com/1420-3049/19/9/13932/pdf, url:http\://www.nature.com/gt/journal/v11/n15/full/3302282a.html] +is_a: ECO:0000012 ! functional complementation evidence + +[Term] +id: ECO:0000015 +name: mutant phenotype evidence +def: "A type of experimental phenotypic evidence in which an observable phenotypic difference results from a change or mutation in DNA." [GO:IMP] +comment: Note that mutations need not be negative. Changes to DNA sequence (mutations) may be detrimental, have no impact, or be beneficial. +comment: The allele that encodes the phenotype most common in a particular natural population is referred to as the wild type allele, while any other form of that allele is known as the mutant form. +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000112 "Indeed, the GroEL protein appears to be more abundant in the LM3 wild type strain compared to the LM3-2 mutant strain, suggesting the involvement of the CcpA protein in the positive regulation of its expression." xsd:string {xref="PMID:17129387"} + +[Term] +id: ECO:0000016 +name: loss-of-function mutant phenotype evidence +def: "A type of mutant phenotype evidence where a phenotype is associated with altered gene product which lacks the molecular function of the wild-type gene." [SO:0002054] +is_a: ECO:0000015 ! mutant phenotype evidence + +[Term] +id: ECO:0000017 +name: ectopic expression evidence +def: "A type of experimental phenotypic evidence where a transgenic strain carrying the construct of a promoter cDNA fusion in which a gene of interest is driven by a defined promoter or enhancer is ectopically expressed in the defined pattern to characterize potential cellular properties and functions of a protein of interest." [PMID:10948520, PMID:19301619] +synonym: "analysis of overexpression/ectopic expression phenotype" RELATED [] +is_a: ECO:0000059 ! experimental phenotypic evidence + +[Term] +id: ECO:0000018 +name: anti-sense experiment evidence +def: "A type of mutant phenotype evidence where a phenotype is observed while expressing an anti-sense version of a gene product in a wild-type (for that gene product) background." [ECO:SN] +synonym: "anti-sense experiments" RELATED [] +is_a: ECO:0000015 ! mutant phenotype evidence + +[Term] +id: ECO:0000019 +name: RNAi evidence +def: "A type of mutant phenotype evidence where an RNA construct is introduced into a cell and the expression of the gene bearing its complementary sequence is suppressed." [ECO:MCC] +synonym: "RNAi experiment" RELATED [] +is_a: ECO:0000015 ! mutant phenotype evidence + +[Term] +id: ECO:0000020 +name: protein inhibition evidence +def: "A type of direct assay evidence based on the inhibition of the molecular function of a protein." [ECO:MCC] +synonym: "specific protein inhibition evidence" NARROW [] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000021 +name: physical interaction evidence +alt_id: ECO:0005025 +def: "A type of experimental evidence that is based on characterization of an interaction between a gene product and another molecule." [ECO:SN] +comment: Molecules interacted with might include protein, nucleic acid, ion, or complex. +xref: MI:0045 "experimental interaction detection" +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000022 +name: co-purification evidence +def: "A type of physical interaction evidence where a cellular component subunit is isolated as part of purification of its larger complex." [TAIR:TED] +synonym: "co-purification" RELATED [] +xref: MI:0025 "copurification" +is_a: ECO:0000021 ! physical interaction evidence + +[Term] +id: ECO:0000023 +name: affinity evidence +def: "A type of physical interaction evidence that depends on the strength of the interaction between two entities." [ECO:MCC, PSI-MI:MI\:0400] +synonym: "ligand binding evidence" NARROW [] +xref: MI:0400 "affinity technology" +is_a: ECO:0000021 ! physical interaction evidence + +[Term] +id: ECO:0000024 +name: protein binding evidence +def: "A type of affinity evidence resulting from the binding of a molecule to a protein or protein complex." [GO:0005515, url:https\://en.wikipedia.org/wiki/Mutation] +is_a: ECO:0000023 ! affinity evidence + +[Term] +id: ECO:0000025 +name: bait-prey hybrid interaction evidence +def: "A type of protein binding evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC, PSI-MI:MI\:0090] +comment: Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode. +synonym: "bait-prey protein pull-down evidence" EXACT [] +xref: MI:0090 "protein complementation assay" +is_a: ECO:0000024 ! protein binding evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string + +[Term] +id: ECO:0000026 +name: nucleic acid hybridization evidence +def: "A type of experimental genomic evidence resulting from a process in which single stranded nucleic acids are allowed to interact so that complexes, or hybrids, are formed by molecules with sufficiently similar, complementary sequences." [OBI:0302903] +comment: This term has been made obsolete with the clean up of experimental genomic evidence branch. +is_obsolete: true + +[Term] +id: ECO:0000027 +name: structural similarity evidence +def: "A type of similarity evidence based on structural similarity of an annotated gene or gene product to another gene or group of genes." [ECO:MCC, TAIR:TED] +comment: For GO annotation, in the case of a single gene, an accession for the related gene's sequence is entered in the evidence_with field. +is_a: ECO:0000057 ! phenotypic similarity evidence +property_value: IAO:0000112 "The secondary structure of VapC10 (Figure S2), predicted with the 3DJIGSAW prediction tool [36] and the DALI server [37], exhibited homology with several well studied VapC toxins, such as the first PIN domain structure for the protein PAE2754 from the archae bacterium Pyrobaculum aerophilum (30)." xsd:string {xref="PMID:24260461"} + +[Term] +id: ECO:0000028 +name: motif similarity evidence +def: "A type of match to sequence model evidence that is based on the presence of a recognized domain or motif in a gene product's (usually protein) primary sequence." [ECO:SN] +synonym: "recognized domains" RELATED [] +is_a: ECO:0000202 ! match to sequence model evidence +property_value: IAO:0000112 "A CRP box-like sequence was found in the promoter-proximate region of sycO-ypkA-yopJ [4], indicating the direct association of CRP with the sycO-ypkA-yopJ promoter region." xsd:string {xref="PMID:19703315"} +property_value: IAO:0000112 "To identify further CopR target genes, the binding motif TGAAGATTTnnTGAAGATTT was used to search for similar sequences in the whole C. glutamicum genome using the ERGO (TM) bioinformatics suite (Integrated Genomics, Illinois, USA) allowing four mutations, no deletions and no insertions. 46 hits were found, but only in six cases the putative CopR binding site was located in intergenic regions up to 200 bp upstream of the start codon of the neighbouring gene (cg1336, cg2976, cg3187, cg3337, cg3357 and cg0414)." xsd:string {xref="PMID:21799779"} + +[Term] +id: ECO:0000029 +name: match to InterPro member signature evidence +def: "A type of match to sequence model evidence resulting from a positive match of a protein, or set of proteins to a predictive model (signature) in the InterPro database." [PMC:2686546, url:http\://www.ncbi.nlm.nih.gov/mesh?term=Nucleic+Acid+Hybridization] +is_a: ECO:0000202 ! match to sequence model evidence + +[Term] +id: ECO:0000030 +name: BLAST evidence used in manual assertion +def: "A type of BLAST evidence that is used in a manual assertion." [ECO:MCC] +synonym: "curated BLAST analysis" RELATED [] +is_a: ECO:0000206 ! BLAST evidence +is_a: ECO:0005560 {is_inferred="true"} ! pairwise sequence alignment evidence used in manual assertion +intersection_of: ECO:0000206 ! BLAST evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISA" xsd:string + +[Term] +id: ECO:0000031 +name: protein BLAST evidence used in manual assertion +def: "A type of protein BLAST evidence that is used in a manual assertion." [ECO:SN] +synonym: "curated protein BLAST analysis" RELATED [] +xref: GO_REF:0000012 "Pairwise alignment (TIGR)" +xref: GO_REF:0000027 "BLAST search criteria for ISS assignment in PAMGO_GAT" +is_a: ECO:0000030 {is_inferred="true"} ! BLAST evidence used in manual assertion +is_a: ECO:0000208 ! protein BLAST evidence +intersection_of: ECO:0000208 ! protein BLAST evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISA" xsd:string + +[Term] +id: ECO:0000032 +name: nucleotide BLAST evidence used in manual assertion +def: "A type of nucleotide BLAST evidence that is used in a manual assertion." [ECO:SN] +synonym: "curated nucleic acid BLAST analysis" RELATED [] +is_a: ECO:0000030 {is_inferred="true"} ! BLAST evidence used in manual assertion +is_a: ECO:0000207 ! nucleotide BLAST evidence +intersection_of: ECO:0000207 ! nucleotide BLAST evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISA" xsd:string + +[Term] +id: ECO:0000033 +name: author statement supported by traceable reference +def: "A type of author statement in which the author makes a statement that is not supported by information in that particular publication, but rather can be traced to a reference cited by that publication." [ECO:RCT, GO:TAS] +synonym: "traceable author statement" RELATED [] +is_a: ECO:0000204 ! author statement +disjoint_from: ECO:0000034 ! author statement without traceable support + +[Term] +id: ECO:0000034 +name: author statement without traceable support +def: "A type of author statement that is not associated with results presented or a cited reference." [ECO:SN] +synonym: "non-traceable author statement" RELATED [] +is_a: ECO:0000204 ! author statement + +[Term] +id: ECO:0000035 +name: no evidence data found +def: "A type of curator inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources." [ECO:SN] +comment: An assertion of "no evidence data found" carries the assumption that a more-or-less exhaustive search has been conducted. +is_a: ECO:0000205 ! curator inference + +[Term] +id: ECO:0000037 +name: not_recorded +comment: The evidence not_recorded appears in some legacy annotations; it should not be used for new annotations. +is_obsolete: true + +[Term] +id: ECO:0000038 +name: transient rescue experiment evidence +def: "A type of functional complementation evidence resulting from the introduction of nucleic acids, which are not permanently incorporated into the genome, to temporarily prevent, or \"rescue\" an organism from a condition." [] +is_a: ECO:0000012 ! functional complementation evidence + +[Term] +id: ECO:0000039 +name: protein assay evidence +alt_id: ECO:0005022 +def: "A type of direct assay evidence resulting from determining the presence, abundance, structure, function, or activity of proteins." [PMID:18429326] +is_obsolete: true + +[Term] +id: ECO:0000040 +name: immunological assay evidence +alt_id: ECO:0005018 +def: "A type of affinity evidence resulting from quantitation of the analyte which depends on the reaction of an antigen (analyte) and an antibody." [ERO:0001362, url:http\://www.ncbi.nlm.nih.gov/books/NBK21589/\,http\://cores.ucsf.edu/protein-assay.html] +is_a: ECO:0000023 ! affinity evidence + +[Term] +id: ECO:0000041 +name: similarity evidence +def: "A type of evidence resulting from comparing likeness of distinct biological entities." [ECO:SN] +synonym: "inferred from similarity" RELATED [] +synonym: "IS" EXACT [] +is_a: ECO:0000000 ! evidence + +[Term] +id: ECO:0000042 +name: gain-of-function mutant phenotypic evidence +def: "A type of mutant phenotype evidence resulting from an altered gene product which possesses a new molecular function or a new pattern of gene expression." [SO:0002053, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3614608/] +synonym: "gain-of-function mutant phenotype evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence + +[Term] +id: ECO:0000044 +name: sequence similarity evidence +def: "A type of similarity based on biomolecular sequence." [ECO:MCC, TAIR:TED] +comment: A sequence similarity analysis may involve a gene or a gene product, and it could be based on similarity to a single other gene or to a group of other genes. +is_a: ECO:0000041 ! similarity evidence +is_a: ECO:0007672 ! computational evidence + +[Term] +id: ECO:0000045 +name: spatial pattern of protein expression evidence +def: "A type of protein expression evidence that accounts for the position of RNA translation to a protein product ranging in scale from differing tissues to regions of an anatomical structure." [] +is_a: ECO:0000010 ! protein expression evidence + +[Term] +id: ECO:0000047 +name: spatial pattern of transcript expression evidence +def: "A type of transcript expression evidence that accounts for the position of transcription ranging in scale from the position of genes on the chromosome to differing tissues to regions of an anatomical structure." [] +is_a: ECO:0000009 ! transcript expression evidence + +[Term] +id: ECO:0000049 +name: reporter gene assay evidence +def: "A type of expression pattern evidence that is based on the expression pattern of a reporter gene." [TAIR:TED] +synonym: "expression of a reporter gene" RELATED [] +is_a: ECO:0000008 ! expression pattern evidence + +[Term] +id: ECO:0000050 +name: voucher specimen phenotypic analysis evidence +def: "A type of experimental phenotypic evidence based on an authors phenotypic description of a species (or higher-level group), which explicitly references an observation made of a voucher specimen (a specimen with a permanent museum catalog)." [TAIR:TED] +synonym: "IVS" EXACT [] +synonym: "voucher specimen analysis evidence" EXACT [] +is_a: ECO:0000059 ! experimental phenotypic evidence + +[Term] +id: ECO:0000051 +name: genetic similarity evidence +def: "A type of similarity based on genotype without respect to expression." [ECO:MCC, PhenoScape:IGTS] +comment: A genetic similarity analysis might consider genetic markers, polymorphisms, alleles, or other characteristics sometimes considered as part of the field of traditional genetics. Although an attempt has been made to treat as distinct the concepts of "genetic", "genotypic", "genomic", and "sequence", there is considerable overlap in usage throughout the field of biology. +synonym: "IGTS" EXACT [] +synonym: "inferred from genetic similarity" RELATED [] +is_a: ECO:0000041 ! similarity evidence + +[Term] +id: ECO:0000052 +name: suppressor/enhancer interaction phenotypic evidence +def: "A type of genetic interaction evidence resulting from a second mutation that either suppresses or enhances the phenotype expressed from an original mutation." [url:http\://biorxiv.org/content/early/2015/10/03/021592, url:http\://www.wormbook.org/chapters/www\:geneticsuppression/geneticsuppression.html] +synonym: "'traditional' genetic interactions (e.g. suppressors, synthetic lethals)" RELATED [] +synonym: "suppressor/enhancer interaction evidence" EXACT [] +xref: TAIR:TED +is_a: ECO:0000011 ! genetic interaction evidence + +[Term] +id: ECO:0000053 +name: automatically integrated combinatorial evidence used in automatic assertion +alt_id: ECO:0000246 +def: "A type of automatically integrated combinatorial evidence that is used in an automatic assertion." [ECO:MCC, ECO:RCT] +comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments. +xref: TAIR:TED +is_a: ECO:0000213 {is_inferred="true"} ! combinatorial evidence used in automatic assertion +is_a: ECO:0007673 {is_inferred="true"} ! automatically integrated combinatorial evidence +intersection_of: ECO:0007673 ! automatically integrated combinatorial evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string + +[Term] +id: ECO:0000054 +name: double mutant phenotypic evidence +def: "A type of mutant phenotype evidence resulting from an experiment typically constructed to determine if two different genes have an observable genetic interaction (functional connection) as the result of a mutation occurring in the alleles of the two genes of interest." [ECO:RCT, PMID:18305163] +synonym: "double mutant analysis" RELATED [] +synonym: "double mutant phenotype evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000112 "In the hns slyA double mutant, hlyE expression was twofold greater than in the parent, and slightly higher than the hns single mutant, suggesting that slyA has a small negative effect on hlyE expressionin the absence of H-NS." xsd:string {xref="PMID:17892462"} + +[Term] +id: ECO:0000055 +name: array experiment evidence +comment: This general 'array experiment' term should not be used - replace with the specific type of array (e.g. ECO:0000097, ECO:0000062, ECO:0000104, etc.) +is_obsolete: true + +[Term] +id: ECO:0000056 +name: epistatic interaction phenotypic evidence +def: "A type of genetic interaction evidence resulting from the suppression of one allelic effect by an allele at another genetic locus." [PMID:18852697] +comment: Epistasis' can be used in different contexts in different areas of genetics. It is sometimes used to mean 'genetic interaction', whereas other times it may be specific to mutations that block the effects of other mutations. +synonym: "epistatic interaction evidence" EXACT [] +synonym: "epistatic interactions" RELATED [] +is_a: ECO:0000011 ! genetic interaction evidence + +[Term] +id: ECO:0000057 +name: phenotypic similarity evidence +def: "A type of similarity based on the expression of a genotype in an environment." [ECO:MCC, PhenoScape:IPTS] +comment: Phenotype is defined as the outcome of the expression of a genotype in a given environment. A comparison might involve whole organisms or sub-parts of organisms. +synonym: "inferred from phenotypic similarity" RELATED [] +synonym: "IPTS" EXACT [] +synonym: "phenotype similarity evidence" EXACT [] +is_a: ECO:0000041 ! similarity evidence + +[Term] +id: ECO:0000058 +name: expression microarray evidence +alt_id: ECO:0000356 +def: "A type of transcript expression evidence resulting from simultaneous profiling of the expression levels of thousands of genes in a single experiment allowing analysis of genes and their networks." [url:http\://www.illumina.com/techniques/microarrays/gene-expression-arrays.html, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435252/] +synonym: "differential gene expression evidence from microarray experiment" NARROW [] +is_a: ECO:0000009 ! transcript expression evidence +property_value: IAO:0000112 "S. lividans AdpA directly regulates at least the six AdpA-dependent genes listed above and identified by microarrays and qRT-PCR analysis." xsd:string {xref="PMID:24694298"} +property_value: IAO:0000112 "To identify the regulon of the response regulator CopR, the transcriptome of the DeltacopRS deletion mutant was compared to that of the wild type using DNA microarrays. For cells grown in standard CGXII medium (1.25 microM CuSO4), no significant gene expression differences were observed, indicating that the CopRS two-component system is not active under this condition (data not shown)." xsd:string {xref="PMID:21799779"} + +[Term] +id: ECO:0000059 +name: experimental phenotypic evidence +alt_id: ECO:0000014 +alt_id: ECO:0005017 +def: "A type of experimental evidence that is based on an observable characteristic trait, which is the result of the expression of an organisms genotype in an environment." [ECO:SN] +comment: Observable characteristic traits can be morphology, development, behavior, biochemical or physiological properties, etc. +synonym: "inferred from phenotype" RELATED [] +is_a: ECO:0000006 ! experimental evidence + +[Term] +id: ECO:0000060 +name: positional similarity evidence +def: "A type of phenotypic similarity evidence based on the similarity of stucture locations or arrangements." [TAIR:TED] +synonym: "IPS" EXACT [] +is_a: ECO:0000057 ! phenotypic similarity evidence + +[Term] +id: ECO:0000061 +name: quantitative trait analysis evidence +def: "A type of phenotypic evidence that is based on a gene product that is associated with a quantitative trait locus, but has not been cloned." [TAIR:TED] +synonym: "QTL analysis evidence" EXACT [] +synonym: "quantitative trait analysis" RELATED [] +is_a: ECO:0000059 ! experimental phenotypic evidence + +[Term] +id: ECO:0000062 +name: cRNA to DNA expression microarray evidence +def: "A type of expression microarray evidence where expression level is quantified by sample biotin-labeled cRNA (transcribed from an unknown RNA sample) hybridized to DNA oligonuclotides immoblized on a solid surface." [url:https\://link.springer.com/chapter/10.1007/978-94-017-9716-0_30] +synonym: "genomic microarray evidence" EXACT [] +is_a: ECO:0000058 ! expression microarray evidence + +[Term] +id: ECO:0000063 +name: compositional similarity evidence +def: "A type of phenotypic similarity evidence based on the similarity of the histological makeup of structures." [TAIR:TED] +synonym: "ICS" EXACT [] +is_a: ECO:0000057 ! phenotypic similarity evidence + +[Term] +id: ECO:0000064 +name: functional complementation in heterologous system evidence +def: "A type of functional complementation evidence that is based on the insertion of a wild-type copy of a gene into a heterologous organism, with the mutation occurring in a homologous gene." [TAIR:TED] +synonym: "functional complementation in heterologous system" RELATED [] +is_a: ECO:0000012 ! functional complementation evidence + +[Term] +id: ECO:0000066 +name: yeast one-hybrid evidence +def: "A type of bait-prey hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors." [ECO:MCC, PSI-MI:MI\:0432, TAIR:TED] +comment: The assay involves screening a library of candidate proteins for the ability bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to a yeast reporter gene (TAIR:TED). +synonym: "yeast one-hybrid assay" RELATED [] +xref: MI:0432 "one hybrid" +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence + +[Term] +id: ECO:0000067 +name: developmental similarity evidence +def: "A type of phenotypic similarity evidence based on the similarity of embryological or post-embryonic orgin of structures." [TAIR:TED] +synonym: "IDS" EXACT [] +is_a: ECO:0000057 ! phenotypic similarity evidence + +[Term] +id: ECO:0000068 +name: yeast 2-hybrid evidence +def: "A type of bait-prey hybrid interaction evidence that is based on detection of protein-protein interaction by activation of a yeast reporter gene after a bait protein fused to a DNA-binding domain (which has been transfected into a yeast cell) is used to screen a cDNA library of clones fused to an activation domain." [PMID:12734586] +synonym: "yeast two-hybrid assay" RELATED [] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence + +[Term] +id: ECO:0000069 +name: differential methylation hybridization evidence +alt_id: ECO:0000065 +def: "A type of in vitro methylation assay evidence where methylation-sensitive restriction enzymes are utilized to compare differential methylation between two samples by first shearing DNA, followed by end-blunting, ligation of linkers, methylation sensitive restriction, PCR using linker primers, dye labeling and relative quantification of methylated DNA fragments by two-colored array hybridization to a CpG island microarray for visual assessment." [PMID:18987809] +comment: Color is indicative of methylation status - be it hypo- (pseudo-green), hyper- (pseudo-red), or equal methylation (pseudo-yellow). +synonym: "CpG island microarray evidence" EXACT [] +is_a: ECO:0001065 ! in vitro methylation assay evidence + +[Term] +id: ECO:0000070 +name: co-immunoprecipitation evidence +def: "A type of immunoprecipitation evidence that involves precipitating two or more proteins via binding to an antibody specific to a single protein, followed by protein identification." [ECO:MCC] +comment: If performing GO annotation, the interacting protein is referenced in the evidence_with column. +synonym: "co-immunoprecipitation" RELATED [] +xref: MI:0019 "coimmunoprecipitation" +is_a: ECO:0000085 ! immunoprecipitation evidence + +[Term] +id: ECO:0000071 +name: morphological similarity evidence +def: "A type of phenotypic similarity evidence based on the similarity of shape, structure, or configuration in structures." [TAIR:TED] +synonym: "IMS" EXACT [] +is_a: ECO:0000057 ! phenotypic similarity evidence + +[Term] +id: ECO:0000072 +name: Sos-recruitment assay evidence +is_obsolete: true + +[Term] +id: ECO:0000073 +name: experimental genomic evidence +def: "A type of experimental evidence that is based on the characterization of an attribute of the genome underlying a gene product." [ECO:MCC] +comment: This term has been made obsolete with the clean up of experimental genomic evidence branch. +synonym: "inferred from genomic analysis" EXACT [] +is_obsolete: true + +[Term] +id: ECO:0000074 +name: split-ubiquitin functional complementation evidence +def: "A type of protein fragment functional complementation evidence that is based on detection of protein-protein interaction between a bait and prey protein by in vivo reconstitution of split-ubiquitin (when bait and prey interact) and release of a reporter protein." [PMID:15064465] +comment: The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquitin (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough to reconstitute.TAIR:TED +synonym: "split-ubiquitin assay" RELATED [] +xref: MI:0112 "ubiquitin reconstruction" +is_a: ECO:0006256 ! protein fragment functional complementation evidence + +[Term] +id: ECO:0000075 +name: gene expression similarity evidence +def: "A type of phenotypic similarity evidence that is based on the categorization of genes by the similarity of expression profiles." [PMID:19958477] +synonym: "IGES" EXACT [] +is_a: ECO:0000057 ! phenotypic similarity evidence + +[Term] +id: ECO:0000076 +name: far-Western blotting evidence +def: "A type of physical interaction evidence that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes." [PMID:18079728] +comment: The interacting protein is referenced in the evidence_with column. +synonym: "far-Western analysis" RELATED [] +xref: MI:0047 +is_a: ECO:0000021 ! physical interaction evidence + +[Term] +id: ECO:0000077 +name: methylation-specific polymerase chain reaction evidence +def: "A type of in vitro methylation assay evidence resulting from initial modification of DNA by sodium bisulfite, converting all unmethylated cytosines to uracil, and subsequent amplification with primers specific for methylated versus unmethylated DNA." [PMC:38513, PMID:8790415] +comment: The product is the amplification of existing sequences - i.e. more DNA that can be further processed e.g. sequenced, DNA fingerprinting. +synonym: "methylation-specific PCR evidence" EXACT [] +synonym: "MS-PCR" EXACT [] +synonym: "MSP" EXACT [] +is_a: ECO:0001065 ! in vitro methylation assay evidence + +[Term] +id: ECO:0000078 +name: southern hybridization evidence +def: "A type of DNA detection assay evidence that is based on detection of a specific DNA sequence by hybridization of labeled probes to any immobilized DNA fragment with sequence similarity." [PMID:18432697] +comment: The DNA fragments are prepared through gel electrophoresis then transferred to a filter membrane. +synonym: "Southern blot" EXACT [] +synonym: "Southern blotting" RELATED [] +is_a: ECO:0005519 ! DNA detection assay evidence + +[Term] +id: ECO:0000079 +name: affinity chromatography evidence +def: "A type of affinity evidence that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bound compound." [ECO:MCC, TAIR:TED] +comment: "Used when an annotation is made based on affinity chromatography, which is a selective separation technique by which a compound (e.g., an antibody) is immobilized on a polymeric matrix and used to bind selectively other compounds. Following removal of the unattached components, the bound compound is displaced by changing the concentration of protons, salts, or cofactors in the eluent" (from original definition by TAIR:TED). Types of highly specific interaction might include those of antigen and antibody, enzyme and substrate, or receptor and ligand. +synonym: "affinity chromatography" RELATED [] +xref: MI:0004 "affinity chromatography technology" +is_a: ECO:0000023 ! affinity evidence + +[Term] +id: ECO:0000080 +name: phylogenetic evidence +def: "A type of similarity that indicates common ancestry." [ECO:MCC, PhenoScape:IP] +synonym: "IP" EXACT [PhenoScape:IP] +is_a: ECO:0000041 ! similarity evidence +property_value: IAO:0000112 "Comparative phylogenomics along with MLST (multilocus sequence typing) and whole genome sequecing has shown that ribotype 078 lineage is different than other C. difficile lineages [22]." xsd:string {xref="PMID:24713082"} + +[Term] +id: ECO:0000081 +name: targeting sequence prediction evidence +def: "A type of motif similarity evidence that is based on detection of a targeting sequence in the primary sequence of a protein through computational prediction and/or a manual examination of the sequence." [ECO:RCT, TAIR:TED] +synonym: "targeting sequence prediction" RELATED [] +is_a: ECO:0000028 ! motif similarity evidence + +[Term] +id: ECO:0000082 +name: polymerase chain reaction evidence +def: "A type of experimental genomic evidence where DNA polymerase is used to synthesize new strand of DNA complementary to the offered template strand." [OBI:0000415] +comment: This term has been made obsolete because PCR is not evidence. The children have been appropriately placed under other parents. +synonym: "PCR evidence" EXACT [] +is_obsolete: true + +[Term] +id: ECO:0000083 +name: transmembrane domain prediction evidence +def: "A type of motif similarity evidence that is based on detection of one, or more, transmembrane domains in the primary sequence of a protein through computational prediction and/or a manual examination of the sequence." [ECO:RCT, TAIR:TED] +synonym: "transmembrane domain prediction" RELATED [] +is_a: ECO:0000028 ! motif similarity evidence + +[Term] +id: ECO:0000084 +name: gene neighbors evidence +def: "A type of genomic context evidence in which a gene product's identity is supported on the basis of the identity of neighboring genes." [ECO:MCC, GOC:MG] +comment: Genomic cluster analyses include synteny and operon structure. +synonym: "ICL" RELATED [] +synonym: "inferred from genome cluster" EXACT [] +xref: GO_REF:0000025 "Operon structure as IGC evidence" +is_a: ECO:0000177 ! genomic context evidence +created_by: mgiglio +creation_date: 2009-03-20T11:55:18Z + +[Term] +id: ECO:0000085 +name: immunoprecipitation evidence +def: "A type of protein binding evidence that involves precipitation of a multivalent antigen by a bivalent antibody for protein isolation." [ECO:MCC, ECO:SW, TAIR:TED] +comment: Transcription factors isolated in this way can be incubated with a radiolabeled probe to demonstrate binding. +synonym: "immunoprecipitation" RELATED [] +is_a: ECO:0000024 ! protein binding evidence +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000086 +name: amplification of intermethylated sites evidence +def: "A type of in vitro methylation assay evidence resulting from a genome-wide estimate of DNA methylation by differential enzymatic digestion of genomic DNA with methylation-sensitive and methylation-insensitive isoschizomers, followed by restrained PCR amplification of sequences methylated at both ends and resolving the PCR products in a denaturing polyacrylamide-sequencing gel to generate fingerprints that consist of multiple anonymous bands that represent the DNA methylome of the cell." [PMID:18987810, url:https\://www.ncbi.nlm.nih.gov/probe/docs/techpcr/] +comment: The bands can be individually isolated and characterized which leads to the identification of hypo- and hypermethylation events. +synonym: "AIMS" EXACT [] +is_a: ECO:0001065 ! in vitro methylation assay evidence + +[Term] +id: ECO:0000087 +name: immunolocalization evidence +def: "A type of microscopy evidence where antibodies are used to detect the location of molecules or other structures within cells or tissues." [ECO:MCC, ECO:SN] +synonym: "immunolocalization" RELATED [] +is_a: ECO:0001098 ! microscopy evidence +is_a: ECO:0005593 ! immunodetection assay evidence + +[Term] +id: ECO:0000088 +name: biological system reconstruction evidence +def: "A type of evidence that is based on a combination of experimental evidences or existing models of that system in a related species used for the reconstruction of a biological system." [ECO:MCC, GOC:mg] +comment: The biological system in question might be a multi-step process or pathway or a physical complex comprising several components. The components in the experimental evidence can come from the same species or a mix of species. The experimental evidences may be only partial or weak. +synonym: "inferred from system reconstruction" RELATED [] +synonym: "ISR" RELATED [] +is_a: ECO:0000000 ! evidence +created_by: mgiglio +creation_date: 2009-03-20T12:00:17Z + +[Term] +id: ECO:0000089 +name: restriction landmark genomic scanning evidence +alt_id: ECO:0001125 +def: "A type of DNA detection assay evidence resulting from the use of restriction enzymes for direct end-labeling of DNA (creating landmarks), followed by high-resolution two-dimensional electrophoresis to visualize the landmarks." [PMID:8388788] +synonym: "restriction landmark genome scanning evidence" EXACT [] +synonym: "RLGS evidence" EXACT [] +is_a: ECO:0005519 ! DNA detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string + +[Term] +id: ECO:0000090 +name: immunogold labelling evidence +def: "A type of immunolocalization evidence where the antibodies are labeled with colloidal gold particles whose location is then detected via microscopy." [ECO:MCC] +synonym: "immunogold labelling" RELATED [] +is_a: ECO:0000087 ! immunolocalization evidence + +[Term] +id: ECO:0000092 +name: epitope-tagged protein immunolocalization evidence +def: "A type of immunolocalization evidence in which recombinant proteins fused with epitopes are recognized by antibodies." [TAIR:TED] +synonym: "immunolocalization of epitope-tagged protein" RELATED [] +is_a: ECO:0000087 ! immunolocalization evidence + +[Term] +id: ECO:0000093 +name: array-based sequence capture evidence +def: "A type of DNA detection assay evidence evidence where gene sequence or variation is determined or detected by fluorescently-labeled DNA fragments hybridized to sequence-specific oligonucleotides immobilized on an array." [PMID:21049075] +synonym: "DNA microarray" BROAD [] +synonym: "oligonucleotide microarray evidence" BROAD [] +synonym: "single nucleotide polymorphism array evidence" NARROW [] +synonym: "SNP array evidence" NARROW [] +is_a: ECO:0005519 ! DNA detection assay evidence + +[Term] +id: ECO:0000094 +name: biological assay evidence +comment: The children of 'biological assay evidence' have been moved under 'direct assay evidence', and this term has been deprecated with no children left. +is_obsolete: true + +[Term] +id: ECO:0000095 +name: cell growth regulation assay evidence +def: "A type of cell growth assay evidence that measures one or more aspects of cell growth over a specified time period to indicate an induced or repressed regulatory effect." [ECO:SW, PomBase:MAH] +comment: Cell growth aspects can include growth rate and extent of growth. A cell growth curve acts as a natural reporter. +synonym: "growth curve analysis" RELATED [] +is_a: ECO:0001563 ! cell growth assay evidence +created_by: mchibucos +creation_date: 2014-10-15T00:58:50Z + +[Term] +id: ECO:0000096 +name: electrophoretic mobility shift assay evidence +def: "A type of affinity evidence based on an electrophoretic mobility shift of macromolecules, where proteins, nucleic acids, or both, are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect interactions/complexes between proteins and/or nucleic acid." [ECO:KIM, ECO:SW, PMID:17703195, TAIR:TED] +comment: EMSA is often used for assessing TF-binding with fluorophore labelling. Protein-nucleic acid complexes generally migrate at a slower rate than the corresponding non-bonded nucleic acid. +synonym: "electrophoretic mobility shift assay" RELATED [] +synonym: "EMSA: electrophoretic mobility shift assay" EXACT [] +synonym: "Gel retardation assay" RELATED [PMID:17703195] +xref: MI:0413 "electrophoretic mobility shift assay" +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000112 "Each of the promoter-proximal regions of qrr2 - 4 was subjected to EMSA with the purified His-OpaR protein (Fig. 6b). The results showed that His-OpaR was able to bind to each of the three DNA fragments tested in a dose-dependent manner in vitro." xsd:string {xref="PMID:22506036"} +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000097 +name: cDNA to DNA expression microarray evidence +alt_id: ECO:0001041 +alt_id: ECO:0005524 +def: "A type of expression microarray evidence where expression level is quantified by fluroescently-labeled cDNA (reverse transcribed from an unknown RNA sample) hybridized to DNA immobilized on a solid surface." [ECO:RCT] +synonym: "DNA microarray" RELATED [] +synonym: "RNA microarray" RELATED [] +is_a: ECO:0000058 ! expression microarray evidence +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000098 +name: obsolete in situ hybridization evidence +def: "A type of nucleic acid hybridization evidence based on localization of a specific segment of DNA or RNA by the application of a complementary strand of nucleic acid to which a reporter molecule is attached." [url:https\://www.ncbi.nlm.nih.gov/probe/docs/techish/] +comment: This term has been made obsolete with the clean up of experimental genomic evidence branch. +synonym: "in situ hybridization" RELATED [] +is_obsolete: true + +[Term] +id: ECO:0000100 +name: fractionation evidence +def: "A type of direct assay evidence resulting from the separation of various cell components while preserving their individual functions." [NBK:26936, url:https\://www.ncbi.nlm.nih.gov/books/NBK26936/] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000101 +name: Affymetrix GeneChip evidence +def: "A type of cRNA to DNA expression microarray evidence resulting from the use of a proprietary Affymetrix GeneChip System for analyzing complex genetic information." [ERO:0001265] +synonym: "Affymetrix array experiment evidence" EXACT [] +is_a: ECO:0000062 ! cRNA to DNA expression microarray evidence + +[Term] +id: ECO:0000102 +name: co-fractionation evidence +def: "A type of fractionation evidence resulting from a protein fractionated with other compounds, factors, or macromolecules." [TAIR:TED] +synonym: "co-fractionation" RELATED [] +is_a: ECO:0000100 ! fractionation evidence + +[Term] +id: ECO:0000104 +name: DNA to cDNA expression microarray evidence +def: "A type of expression microarray evidence where expression levels are quantified by hybridization of fluorescently-labeled DNA to cDNA (reverse transcribed from an unknown RNA sample) immobilized on a solid surface." [ECO:RCT, PMID:15329382] +comment: REM is a 'reverse-format' microarray, where the unknown sample is immoblized to a surface and known DNA is hybridized to that. {xref="PMID:15329382"} +synonym: "microarray RNA expression level evidence" EXACT [] +synonym: "REM evidence" EXACT [] +synonym: "RNA expression microarray evidence" EXACT [] +synonym: "transcript levels (e.g. microarray data)" RELATED [] +is_a: ECO:0000058 ! expression microarray evidence + +[Term] +id: ECO:0000105 +name: Nimblegen array evidence +def: "A type of cDNA to DNA expression microarray evidence resulting from the use of a proprietary NimbleGen array to measure expression levels." [PMID:15607417, url:https\://roche-biochem.jp/pdf/products/microarray/user_guide/SeqCap/SeqCap_UserGuide_Delivery_ver3.0.pdf] +is_a: ECO:0000097 ! cDNA to DNA expression microarray evidence + +[Term] +id: ECO:0000106 +name: northern blot evidence +def: "A type of transcript expression evidence based on electrophoresis and probing to determine levels of RNA expression using a complementary hybridization probe on the separated RNA samples." [ECO:SW, TAIR:TED] +synonym: "northern assay evidence" EXACT [] +synonym: "RNA blot evidence" EXACT [] +synonym: "transcript levels (e.g. Northerns)" RELATED [] +is_a: ECO:0000009 ! transcript expression evidence +property_value: IAO:0000112 "Northern blot analysis indicated that there was one major band of 1.5-kb (which was used for the stability determination) and two other minor bands of approximately 3.0- and 6.0-kb, which constituted less than 5% of the total signal (Fig. 1C), suggesting that other genes may be cotranscribed along with SMU.1882." xsd:string {xref="PMID:21124877"} +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000109 +name: reverse transcription polymerase chain reaction evidence +alt_id: ECO:0000108 +def: "A type of RNA detection assay evidence where an RNA transcript is reverse transcribed into cDNA and amplified to qualitatively detect gene expression." [ECO:SW, PMID:11013345, PMID:12901609] +comment: The starting product for PCR, and therefore amplification volume, is directly correlated to the transcription rate. +synonym: "reverse transcription polymerase chain reaction transcription evidence" BROAD [] +synonym: "RT-PCR" EXACT [] +synonym: "transcript levels (e.g. RT-PCR)" RELATED [] +is_a: ECO:0005518 ! RNA detection assay evidence +property_value: IAO:0000112 "Taken together, the results of the RT-PCR analyses and the reporter fusion assays indicate that expression of SMU.1882 is up regulated in the presence of CovR." xsd:string {xref="PMID:21124877"} +property_value: IAO:0000112 "The RT-PCR assay indicated that the sycO, ypkA and yopJ genes (designated as pCD12, pCD13 and pCD14 in Y. pestis 91001 [19], respectively) were transcribed as a single primary RNA (Fig. 1), and thereby these three genes constituted a single operon in Y. pestis Microtus strain 201." xsd:string {xref="PMID:19703315"} +property_value: IAO:0000112 "Using reverse transcription-PCR, we found that primers designed to span the intergenic regions between zur and the znuC homolog, as well as znuC and znuB homolog each generated a PCR product (Figure 2A). This indicated that these genes were transcribed on the same mRNA." xsd:string {xref="PMID:24086521"} +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000110 +name: RNA protection assay evidence +def: "A type of nuclease protection assay evidence where mRNA is hybridized with radiolabeled RNA probes after which RNAse is added to digest the unbound, nonresistant single-stranded overhang regions, later the remaining probe target hybrids are purified and resolved on denaturing polyacrylamide gel, and quantified by autoradiography." [ECO:SW, PMID:23457339, TAIR:TED] +synonym: "ribonuclease protection assay" EXACT [] +synonym: "RNA protection assay" RELATED [] +synonym: "RNAse protection assay" RELATED [] +synonym: "RPA" NARROW [] +is_a: ECO:0001106 ! nuclease protection assay evidence +property_value: IAO:0000112 "fur RPAs were performed on RNA isolated from each strain, and an increase in fur expression was seen for G27, 26695, and the Fur swap strain under iron-depletion shock conditions while little to no increase was seen under iron-limited growth conditions (Fig. 4D and data not shown). This data shows that Fur autoregulation is consistent in each strain and further supports the notion that the AA difference in Fur is not responsible for the difference in sodB regulation between G27 and 26695." xsd:string {xref="PMID:19399190"} +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000112 +name: qualitative western immunoblotting evidence +alt_id: ECO:0005602 +def: "A type of protein expression evidence where proteins are separated using gel electrophoresis, blotted onto a membrane, and probed with an antibody that can be detected via light or radioactive emission." [ECO:SW, PMID:23050259, TAIR:TED] +comment: Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies. +synonym: "protein expression level evidence based on western blot" EXACT [] +synonym: "protein immunoblot" RELATED [] +synonym: "protein levels (e.g. Western blots)" RELATED [] +synonym: "Western blot expression analysis" RELATED [] +is_a: ECO:0000010 ! protein expression evidence +is_a: ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence +is_a: ECO:0005593 ! immunodetection assay evidence +property_value: IAO:0000112 "When we determined the proteolysis role of Lons in VapBC10 proteins by Western blot analysis using the strains BL21(DE3)(pJS882) and BL21(DE3)(pJS427), the levels of VapB10 and VapC10 remained stable over the course of translation inhibition (Figure 7D and E). Thus, Lon could not degrade VapBC10 proteins, consistent with our drop growth evidence (Figure 6B)." xsd:string {xref="PMID:24260461"} +property_value: IAO:0000234 "CollecTF" xsd:string + +[Term] +id: ECO:0000114 +name: expression library screen evidence +def: "A type of protein expression evidence where a protein of interest is isolated on the basis of its hybridization to an antibody raised to a homologous protein." [ECO:MCC, TAIR:TED] +synonym: "expression library screening" RELATED [] +is_a: ECO:0000010 ! protein expression evidence + +[Term] +id: ECO:0000116 +name: differential hybridization evidence +def: "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue is compared to another cell type or tissue when mRNA from each sample is reverse transcribed, labeled, and hybridized to a cDNA library to compare hybridization patterns." [PMID:14668817] +comment: Differential hybridization identifies differentially expressed cloned genes. Two different mRNA-derived probes are used to demonstrate RNA expression under a specific condition. +synonym: "differential hybridization" RELATED [] +is_a: ECO:0000009 ! transcript expression evidence + +[Term] +id: ECO:0000118 +name: subtractive hybridization evidence +def: "A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue are compared to another cell type or tissue when denatured ds-cDNA from the two samples are hybridized, and the common sequences are 'subtracted'." [PMID:11298187] +comment: Subtractive hybridization is used for isolation and identification of transcripts involving the removal of any sequences present in the control from the test probe to detect only transcripts present in the sample. The method is PCR-based for the comparison of two cDNA libraries. +synonym: "subtractive hybridization" RELATED [] +is_a: ECO:0000009 ! transcript expression evidence + +[Term] +id: ECO:0000120 +name: over expression analysis evidence +def: "A type of experimental phenotypic evidence in which a gene and/or gene product is investigated in a transgenic organism that has been engineered to overexpress that gene product." [PMID:22419077] +synonym: "analysis of overexpression/ectopic expression phenotype" RELATED [] +is_a: ECO:0005027 ! genetic transformation evidence +property_value: IAO:0000112 "In contrast, when RpoQ was overexpressed in either the wild type or the DeltalitR mutant, both were essentially nonmotile (Fig. 7), suggesting that quorum signaling regulates motility, at least in part, through an RpoQ mechanism downstream of LitR." xsd:string {xref="PMID:22233679"} + +[Term] +id: ECO:0000122 +name: protein localization evidence +def: "A type of localization evidence where sub-cellular localization of a protein is determined." [GO:0008104, url:http\://journals.plos.org/plosone/article?id=10.1371/journal.pone.0019937] +is_a: ECO:0001533 ! localization evidence + +[Term] +id: ECO:0000124 +name: fusion protein localization evidence +def: "A type of protein localization evidence resulting from the fusion of a protein of interest to a labeling protein which has enzymatic activity or fluorescence properties." [MI:0240] +is_a: ECO:0000122 ! protein localization evidence + +[Term] +id: ECO:0000126 +name: green fluorescent protein fusion protein localization evidence +def: "A type of fusion protein localization evidence resulting from the labeling of a protein of interest with green fluorescent protein (GFP) from the jellyfish Aequorea victoria." [PMID:9759496] +synonym: "GFP fusion protein localization evidence" EXACT [] +synonym: "localization of GFP/YFP fusion protein" RELATED [] +is_a: ECO:0000124 ! fusion protein localization evidence + +[Term] +id: ECO:0000128 +name: yellow fluorescent protein fusion protein localization evidence +def: "A type of fusion protein localization evidence resulting from the labeling of a protein of interest with yellow fluorescent protein (YFP), which was created by mutating green fluorescent protein (GFP) of the Aequorea victoria." [MI:0368, url:http\://www.jbc.org/content/276/31/29188.long] +synonym: "localization of GFP/YFP fusion protein" RELATED [] +synonym: "YFP fusion protein localization evidence" EXACT [] +is_a: ECO:0000124 ! fusion protein localization evidence + +[Term] +id: ECO:0000130 +name: beta-glucuronidase fusion protein localization evidence +def: "A type of fusion protein localization evidence resulting from the targeted insertion of the beta-glucuronidase (GUS) coding gene as a reporter gene for the localization of a particular gene product." [ECO:RCT] +synonym: "GUS fusion protein localization evidence" EXACT [] +synonym: "GUS staining evidence" NARROW [] +synonym: "localization of GUS fusion protein" RELATED [] +is_a: ECO:0000124 ! fusion protein localization evidence + +[Term] +id: ECO:0000132 +name: beta-galactosidase fusion protein localization evidence +def: "A type of fusion protein localization evidence resulting from the targeted insertion of the beta-galactosidase coding gene (lacZ) into a specific gene to create a beta-galactosidase-tagged fusion protein." [PMID:23681629] +synonym: "LacZ fusion protein localization evidence" EXACT [] +is_a: ECO:0000124 ! fusion protein localization evidence + +[Term] +id: ECO:0000134 +name: transport assay evidence +def: "A type of direct assay evidence resulting from the activity assessment of transport proteins." [PMID:18401524] +synonym: "transport assay" RELATED [] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000136 +name: nucleic acid binding evidence +def: "A type of affinity evidence resulting from the binding of a molecule to a nucleic acid." [GO:0003676] +is_a: ECO:0000023 ! affinity evidence + +[Term] +id: ECO:0000138 +name: ribohomopolymer binding assay evidence +def: "A type of nucleic acid binding evidence resulting from an enzyme displaying binding activity to specific ribohomopolymer." [PMC:102612] +synonym: "ribohomopolymer binding assay" RELATED [] +is_a: ECO:0000136 ! nucleic acid binding evidence + +[Term] +id: ECO:0000140 +name: thin layer chromatography evidence +def: "A type of chromatography evidence that uses a thin layer of adsorbent (such as silica gel, alumina, or cellulose) on a flat, inert substrate." [ECO:MCC] +synonym: "thin layer chromatography" RELATED [] +synonym: "TLC evidence" EXACT [] +is_a: ECO:0000325 ! chromatography evidence + +[Term] +id: ECO:0000142 +name: protein:ion binding evidence +def: "A type of protein binding evidence resulting from a metal ion binding to a protein at a specific binding site." [PMID:2377604] +is_a: ECO:0000024 ! protein binding evidence + +[Term] +id: ECO:0000144 +name: Southwestern blot evidence +def: "A type of nucleic acid binding evidence in which DNA-protein binding is detected using labeled DNA as probes, hybridized to electrophoretically separated proteins." [ECO:RCT, TAIR:TED] +synonym: "Southwestern analysis" RELATED [] +is_a: ECO:0000136 ! nucleic acid binding evidence + +[Term] +id: ECO:0000146 +name: Northwestern blot evidence +def: "A type of nucleic acid binding evidence in which RNA-protein binding is detected using labeled RNA as probes, hybridized to electrophoretically separated proteins." [ECO:RCT, TAIR:TED] +synonym: "Northwestern analysis" RELATED [] +is_a: ECO:0000136 ! nucleic acid binding evidence + +[Term] +id: ECO:0000148 +name: in vitro binding evidence +is_obsolete: true + +[Term] +id: ECO:0000150 +name: in vitro transcription reconstitution assay evidence +def: "A type of reconstitution assay evidence resulting from the use of reconstituted polymerase transcription systems to investigate RNA synthesis." [PMID:9237163] +synonym: "in vitro reconstitution assay with recombinant protein" RELATED [] +is_a: ECO:0000003 ! reconstitution assay evidence +property_value: IAO:0000112 "As shown in Fig. 5, addition of reconstituted RNA polymerase holoenzyme, but not the core enzyme, generated expected size bands of 193-nt and 158-nt when P1882 and Pami, respectively, were used as template (lanes 2 and 7). However, the addition of CovR did not lead to any observable increase in transcription from P1882." xsd:string {xref="PMID:21124877"} + +[Term] +id: ECO:0000152 +name: in vitro recombinant protein transcription reconstitution assay evidence +def: "A type of in vitro transcription reconstitution assay evidence resulting from the use of recombinant proteins in preparation of a fully-defined transcription system." [PMID:9237165] +synonym: "in vitro reconstitution assay with recombinant protein" RELATED [] +is_a: ECO:0000150 ! in vitro transcription reconstitution assay evidence + +[Term] +id: ECO:0000154 +name: heterologous protein expression evidence +def: "A type of protein expression evidence where a gene from one cell is inserted into a cell that does not typically contain that gene and heterologous protein expression is assessed." [ECO:KAV] +synonym: "protein expression in heterologous system" RELATED [] +is_a: ECO:0000010 ! protein expression evidence + +[Term] +id: ECO:0000156 +name: protein separation evidence +def: "A type of fractionation evidence resulting from the isolation of a single type of protein from a complex mixture." [ERO:0000526] +is_a: ECO:0000100 ! fractionation evidence + +[Term] +id: ECO:0000158 +name: protein separation followed by direct sequencing evidence +def: "A type of protein separation evidence that is followed by direct protein sequencing whereby a protein's amino acid sequence is determined experimentally by Edman degradation or by mass spectrometry." [] +synonym: "protein separation and direct sequencing" RELATED [] +is_a: ECO:0000156 ! protein separation evidence + +[Term] +id: ECO:0000160 +name: protein separation followed by fragment identification evidence +def: "A type of protein separation evidence which then utilizes the screening and identification of small-molecules that bind to proteins." [https://pubs.acs.org/doi/10.1021/bi3005126, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5632530/] +synonym: "protein separation and fragment identification" RELATED [] +is_a: ECO:0000156 ! protein separation evidence + +[Term] +id: ECO:0000162 +name: heterologous system uptake evidence +def: "A type of transport assay evidence in which the uptake mechanism of a transporter is investigated in a cell that does not normally express that protein." [doi:10.1146/annurev.pp.46.060195.002223, ECO:RCT] +comment: The transporter is a recombinant protein. +synonym: "uptake assay in heterologous system" RELATED [] +is_a: ECO:0000134 ! transport assay evidence + +[Term] +id: ECO:0000164 +name: electrophysiology assay evidence +def: "A type of direct assay evidence where electrical properties of cells or tissues are studied." [ECO:KAV] +comment: The scale of electrophysiology assays can range from small, e.g. a single ion channel, to large, e.g. an entire organ such as a heart. +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000166 +name: two-electrode voltage clamp recording evidence +def: "A type of voltage clamp recording evidence which involves using two electrodes inserted into a large cell (often a xenopus oocyte) where one electrode is used to measure the internal potential (voltage) of the cell and the other electrode is used to clamp the current." [ECO:SN, PMID:23529422] +synonym: "two-electrode voltage clamp technique" RELATED [] +is_a: ECO:0005576 ! voltage clamp recording evidence + +[Term] +id: ECO:0000168 +name: transcription assay evidence +def: "A type of direct assay evidence resulting from the detection and quantification of transcribed RNA." [MeSH:D014158, url:https\://bmcmolbiol.biomedcentral.com/articles/10.1186/1471-2199-15-7] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000170 +name: transcriptional activation assay evidence +def: "A type of transcription assay evidence in which sequence-specific binding proteins that increase transcription of a specific target gene or genes are identified, quantified, and/or analyzed." [url:http\://smcg.ccg.unam.mx/enp-unam/05-TranscripYRegulacion/transcription.pdf] +synonym: "transcriptional activation assay" RELATED [] +is_a: ECO:0000168 ! transcription assay evidence + +[Term] +id: ECO:0000172 +name: biochemical trait analysis evidence +def: "A type of experimental phenotypic evidence in which the biochemical aspect of a mutation is analyzed." [ECO:RCT] +comment: For example, the accumulation of a biosynthetic intermediate. +synonym: "analysis of biochemical trait" RELATED [] +is_a: ECO:0000059 ! experimental phenotypic evidence + +[Term] +id: ECO:0000174 +name: mutant physiological response evidence +def: "A type of mutant phenotype evidence in which the physiological response of a mutant when presented with an external stimulus is determined." [ECO:RCT] +comment: Examples include: abnormal growth of the root in response to gravity, delay in flowering in response to varying light conditions. +synonym: "analysis of physiological response" RELATED [] +is_a: ECO:0000015 ! mutant phenotype evidence + +[Term] +id: ECO:0000176 +name: mutant visible phenotype evidence +def: "A type of mutant phenotype evidence in which traits arising from a mutation are visually examined." [ECO:RCT] +synonym: "analysis of visible trait" RELATED [] +is_a: ECO:0000015 ! mutant phenotype evidence + +[Term] +id: ECO:0000177 +name: genomic context evidence +def: "A type of similarity evidence in which the location of the gene within the genome provides insight into the gene's function or evolutionary insight." [PMID:10958625, PMID:21609955] +comment: This type of evidence might include identity of neighboring genes, operon structure, synteny, phylogenetic analysis, or other whole-genome analysis. +is_a: ECO:0000041 ! similarity evidence + +[Term] +id: ECO:0000178 +name: in vivo assay evidence +def: "A type of direct assay evidence resulting from a sample of microorganisms or living tissue grown in a medium." [ECO:RCT, url:https\://www.cancer.gov/publications/dictionaries/cancer-terms?cdrid=46352] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000179 +name: animal model system study evidence +def: "A type of experimental evidence arising from the investigation of an animal model system." [ECO:MCC] +is_a: ECO:0000006 ! experimental evidence + +[Term] +id: ECO:0000180 +name: clinical study evidence +def: "A type of experimental evidence arising from a controlled investigation that uses human subjects." [ECO:MCC] +is_a: ECO:0000006 ! experimental evidence + +[Term] +id: ECO:0000181 +name: in vitro assay evidence +alt_id: ECO:0005013 +def: "A type of direct assay evidence resulting from a process performed outside the living organism, where the components of an organism have been isolated from their usual biological context, to permit a more detailed or more convenient analysis in an artificial setting." [ECO:RCT, url:https\://www.cancer.gov/publications/dictionaries/cancer-terms?cdrid=45733] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000182 +name: in vitro culture assay evidence +comment: Use ECO:0001563 (cell growth assay evidence) in place of this term. +is_obsolete: true + +[Term] +id: ECO:0000183 +name: cell-free assay evidence +def: "A type of direct assay evidence resulting from studies in which cytoplasmic and/or nuclear cellular components from resting, nonstimulated cells, are isolated by ultracentrifugation to provide molecular machinery that can be used in reactions in the absence of many of the other cellular components for the purpose of in vitro protein synthesis, transcription, DNA replication, etc." [PMID:18453125] +synonym: "in vitro assay" BROAD [] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0000184 +name: enzyme inhibition evidence +def: "A type of protein inhibition evidence and enzymatic acitivty evidence where the protein under study is an enzyme." [ECO:MCC] +is_a: ECO:0000005 ! enzymatic activity assay evidence +is_a: ECO:0000020 ! protein inhibition evidence + +[Term] +id: ECO:0000200 +name: sequence alignment evidence +alt_id: ECO:00000057 +def: "A type of sequence similarity evidence in which an annotation is made based on a manually-reviewed or published sequence alignment" [url:http\://www.geneontology.org/GO.evidence.shtml] +comment: Such alignments may be pairwise alignments or multiple alignments. +is_a: ECO:0000044 ! sequence similarity evidence +created_by: mchibucos +creation_date: 2010-03-18T12:21:31Z + +[Term] +id: ECO:0000201 +name: sequence orthology evidence +alt_id: ECO:00000060 +def: "A type of sequence similarity evidence in which orthology is established by multiple criteria, including amino acid and/or nucleotide sequence comparisons and one or more of the following: phylogenetic analysis, coincident expression, conserved map location, functional complementation, immunological cross-reaction, similarity in subcellular localization, subunit structure, substrate specificity, and response to specific inhibitors." [url:http\://www.geneontology.org/GO.evidence.shtml] +comment: Orthology is a relationship between genes in different species indicating that the genes derive from a common ancestor. +synonym: "ortholog evidence" RELATED [] +is_a: ECO:0000044 ! sequence similarity evidence +created_by: mchibucos +creation_date: 2010-03-18T12:30:06Z + +[Term] +id: ECO:0000202 +name: match to sequence model evidence +alt_id: ECO:00000063 +def: "A type of sequence similarity evidence in which the function of a gene product is predicted based on a sequence-based statistical model." [ECO:MCC] {comment="url:http://www.geneontology.org/GO.evidence.shtml"} +comment: Used when evidence from any kind of statistical model of a sequence or group of sequences is used to make a prediction about the function of a protein or RNA. Examples of relevant evidence are: Hidden Markov Models, PROSITE motifs, and tools such as tRNASCAN. +is_a: ECO:0000044 ! sequence similarity evidence +property_value: IAO:0000112 "A putative rho-independent terminator sequence (GCCTGACTACATAGATGTCAGGC) was identified at position 402-bp downstream of SMU.1882 by TransTerm (transterm.dev.java.net) program." xsd:string {xref="PMID:21124877"} +created_by: mchibucos +creation_date: 2010-03-18T12:32:30Z + +[Term] +id: ECO:0000203 +name: automatic assertion +alt_id: ECO:00000067 +def: "An assertion method that does not involve human review." [ECO:MCC] +comment: An automatic assertion is based on computationally generated information that is not reviewed by a person prior to making the assertion. For example, one common type of automatic assertion involves creating an association of evidence with an entity based on commonness of attributes of that entity and another entity for which an assertion already exists. The commonness is determined algorithmically. +is_a: ECO:0000217 ! assertion method +disjoint_from: ECO:0000218 ! manual assertion +created_by: mchibucos +creation_date: 2010-03-18T12:36:04Z + +[Term] +id: ECO:0000204 +name: author statement +alt_id: ECO:0007010 +def: "A type of documented statement evidence that is based on an assertion by the author of a paper, which is read by a curator." [ECO:MCC] +is_a: ECO:0006151 ! documented statement evidence +union_of: ECO:0000033 ! author statement supported by traceable reference +union_of: ECO:0000034 ! author statement without traceable support +created_by: mchibucos +creation_date: 2010-06-21T11:17:21Z + +[Term] +id: ECO:0000205 +name: curator inference +def: "A type of inferential evidence that is based on a conclusion drawn by a curator." [ECO:MCC] +is_a: ECO:0000361 ! inferential evidence +created_by: mchibucos +creation_date: 2010-08-18T05:55:12Z + +[Term] +id: ECO:0000206 +name: BLAST evidence +def: "A type of pairwise sequence alignment evidence obtained with basic local alignment search tool (BLAST)." [ECO:MCC] +is_a: ECO:0005554 ! pairwise sequence alignment evidence +created_by: mchibucos +creation_date: 2010-08-06T04:48:24Z + +[Term] +id: ECO:0000207 +name: nucleotide BLAST evidence +def: "A type of BLAST evidence in which nucleotide sequences are aligned." [ECO:MCC] +is_a: ECO:0000206 ! BLAST evidence +created_by: mchibucos +creation_date: 2010-08-06T04:49:58Z + +[Term] +id: ECO:0000208 +name: protein BLAST evidence +def: "A type of BLAST evidence in which amino acid or translated nucleotide sequences are aligned." [ECO:MCC] +is_a: ECO:0000206 ! BLAST evidence +property_value: IAO:0000112 "Blast search of S. meliloti genome for homologues of X. campestris Ohr protein revealed two paralogues, SMa2389 and SMc00040, showing 52 and 57% identity respectively with Ohr of X. campestris." xsd:string {xref="PMID:21569462"} +created_by: mchibucos +creation_date: 2010-08-06T04:50:45Z + +[Term] +id: ECO:0000209 +name: BLAST evidence used in automatic assertion +def: "A type of BLAST evidence that is used in an automatic assertion." [ECO:MCC] +is_a: ECO:0000206 ! BLAST evidence +is_a: ECO:0005561 {is_inferred="true"} ! pairwise sequence alignment evidence used in automatic assertion +intersection_of: ECO:0000206 ! BLAST evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-08-06T04:55:21Z + +[Term] +id: ECO:0000210 +name: nucleotide BLAST evidence used in automatic assertion +def: "A type of nucleotide BLAST evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000207 ! nucleotide BLAST evidence +is_a: ECO:0000209 {is_inferred="true"} ! BLAST evidence used in automatic assertion +intersection_of: ECO:0000207 ! nucleotide BLAST evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-08-06T05:09:51Z + +[Term] +id: ECO:0000211 +name: protein BLAST evidence used in automatic assertion +def: "A type of protein BLAST evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000208 ! protein BLAST evidence +is_a: ECO:0000209 {is_inferred="true"} ! BLAST evidence used in automatic assertion +intersection_of: ECO:0000208 ! protein BLAST evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-08-06T05:11:04Z + +[Term] +id: ECO:0000212 +name: combinatorial evidence +alt_id: ECO:0000036 +alt_id: ECO:0000043 +def: "A type of evidence in which at least two distinct types of evidence have been integrated." [ECO:MCC, ECO:RCT] +comment: A key aspect of this type of evidence is that two or more pieces of information are combined to generate an emergent type of evidence not possible with the constituent pieces of evidence alone. A combinatorial analysis typically involves incorporation of different types of evidence. +synonym: "inferred from in-silico analysis" RELATED [] +is_a: ECO:0000000 ! evidence +intersection_of: ECO:0000000 ! evidence +intersection_of: BFO:0000051 ECO:0000000 ! evidence +created_by: mchibucos +creation_date: 2010-08-06T05:24:57Z + +[Term] +id: ECO:0000213 +name: combinatorial evidence used in automatic assertion +def: "A type of combinatorial analysis that is used in an automatic assertion." [ECO:MCC] +comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments. +synonym: "inferred from in-silico analysis" RELATED [] +is_a: ECO:0000212 ! combinatorial evidence +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +intersection_of: ECO:0000212 ! combinatorial evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-08-06T05:28:33Z + +[Term] +id: ECO:0000214 +name: biological aspect of descendant evidence +def: "A type of phylogenetic evidence whereby an aspect of an ancestral gene is inferred through the characterization of an aspect of a descendant gene." [ECO:MCC] +comment: This type of evidence can be used in support of both positive and "not" GO annotations. It should be used to annotate ancestral genes but not extant ones. +is_a: ECO:0000080 ! phylogenetic evidence +created_by: mchibucos +creation_date: 2010-10-05T11:16:50Z + +[Term] +id: ECO:0000215 +name: rapid divergence from ancestral sequence evidence +def: "A type of phylogenetic evidence characterized by long phylogenetic tree branch lengths following a duplication event." [ECO:MCC] +comment: Long branch lengths indicate that descendant sequences may have acquired different or additional functions than the ancestral sequence. +is_a: ECO:0000080 ! phylogenetic evidence +created_by: mchibucos +creation_date: 2010-10-05T03:41:34Z + +[Term] +id: ECO:0000216 +name: phylogenetic determination of loss of key residues evidence +def: "A type of phylogenetic evidence characterized by the absence of key sequence residues." [ECO:MCC] +comment: The loss of certain residues important for function can indicate that a sequence lacks a particular function. +is_a: ECO:0000080 ! phylogenetic evidence +created_by: mchibucos +creation_date: 2010-10-05T03:43:17Z + +[Term] +id: ECO:0000217 +name: assertion method +def: "A means by which a statement is made about an entity." [ECO:MCC] +union_of: ECO:0000203 ! automatic assertion +union_of: ECO:0000218 ! manual assertion +created_by: mchibucos +creation_date: 2010-11-12T04:17:37Z + +[Term] +id: ECO:0000218 +name: manual assertion +def: "An assertion method that involves human review." [ECO:MCC] +comment: A manual assertion could be based on evidence that is generated by and interpreted by a human or it could involve human review of computationally generated information. +is_a: ECO:0000217 ! assertion method +created_by: mchibucos +creation_date: 2010-11-12T04:20:02Z + +[Term] +id: ECO:0000219 +name: nucleotide sequencing assay evidence +def: "A type of sequencing assay that determines the sequence of a biopolymer comprised of nucleotides." [ECO:MCC] +synonym: "DNA sequencing" RELATED [OBI:0000626] +is_a: ECO:0000220 ! sequencing assay evidence +created_by: mchibucos +creation_date: 2010-11-15T12:22:40Z + +[Term] +id: ECO:0000220 +name: sequencing assay evidence +def: "A type of experimental evidence where the order of molecules that constitute a biopolymer is determined." [ECO:MCC, OBI:0600047] +is_a: ECO:0000006 ! experimental evidence +created_by: mchibucos +creation_date: 2010-11-15T01:44:45Z + +[Term] +id: ECO:0000221 +name: high throughput nucleotide sequencing assay evidence +def: "A type of nucleotide sequencing assay evidence in which a sequence is derived from a parallelized sequencing technique." [ECO:MCC] +comment: Typically thousands to millions of reads are produced in parallel. +synonym: "next generation sequencing evidence" RELATED [] +is_a: ECO:0000219 ! nucleotide sequencing assay evidence +created_by: mchibucos +creation_date: 2010-11-15T02:19:34Z + +[Term] +id: ECO:0000222 +name: Illumina sequencing evidence +def: "A type of high throughput nucleotide sequencing evidence in which sequence is determined through hybridization of adaptor-ligated DNA to a glass slide on which each fragment is simultaneously clonally amplified, followed by progressive rounds of base incorporation with fluorescently-tagged nucleotides, washing, imaging, and cleavage." [OBI:0000724, PMID:26000844] +comment: In Illumina sequencing technology, adapters are ligated onto the ends of randomly fragmented DNA. After single-stranded fragments are bound to the inside surface of a flow cell channel, they undergo in-place bridge PCR amplification. To begin the sequencing cycle, labeled reversible terminators, primers and DNA polymerase are added, followed by laser excitation of the fluorescent label and high resolution scanning. After non-incorporated nucleotides are washed away and the dye is chemically removed, the next cycle repeats with four labeled reversible terminators, primers and DNA polymerase, again followed by imaging. +synonym: "Solexa sequencing result" RELATED [OBI:0000724] +is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence +created_by: mchibucos +creation_date: 2010-11-15T02:38:03Z + +[Term] +id: ECO:0000223 +name: 454 pyrosequencing evidence +def: "A type of high throughput nucleotide sequencing evidence in which the sequence for a ssDNA is determined by addition of primers and then nucleotides, one base pair at a time (which generate a specific fluorescence), to the target strand." [EFO:0005016] +comment: 454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA.\n\nThe target strand is usually prepared by fragmentation, adaptor ligation, and amplification by emulsion PCR. +synonym: "pyrosequencing" BROAD [OBI:0000730] +is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence +created_by: mchibucos +creation_date: 2010-11-15T03:50:14Z + +[Term] +id: ECO:0000224 +name: SOLiD sequencing evidence +def: "A type of high throughput nucleotide sequencing evidence in which sequence is determined through rounds of ligation-based sequencing with fluorescently-labeled Di-base probes and cleavage, after which the template is reset, with a primer offset by one base, for subsequent rounds of ligation." [OBI:0000706] +comment: SOLiD sequencing technology is based on sequencing by ligation. After library preparation, emulsion PCR and bead enrichment are performed, including 3' modification of templates on selected beads to allow covalent bonding to a slide. 3' modified beads are attached to a glass slide and primers are added. Four fluorescently labeled di-base probes are added and multiple cycles of ligation, detection and cleavage are performed. The number of cycles determines read length. After a number of ligation cycles, the extension product is removed and another round of ligation cycles is performed with a new primer complimentary to the n-1 position of the template. Five rounds of primer reset are performed for each sequence tag.\n\nSamples are prepared by fragmentation of a sample library, adaptor ligation, emulsion PCR, and attachment of resultant beads to glass slides. +is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence +created_by: mchibucos +creation_date: 2010-11-15T04:17:41Z + +[Term] +id: ECO:0000225 +name: chain termination sequencing evidence +def: "A type of nucleotide sequencing assay in which DNA sequence is determined by addition of a primed DNA template to a reaction mixture, followed by addition of DNA polymerase, deoxynucleosidetriphosphates (dNTPs - one of which may be radiolabeled), and one of four di-deoxynucleotidetriphosphates (ddNTPs), after which DNA elongation is terminated and the DNA is separated by size and imaged." [OBI:0000695] +comment: Chain termination sequencing, also called Sanger sequencing after its developer, uses dideoxynucleotide triphosphates (ddNTPs) as DNA chain terminators. Single-stranded DNA template, DNA primer, DNA polymerase, fluorescently or radioactively labeled nucleotides, and one of four ddNTPs are added together in each of four separate sequencing reactions. The synthesized and labeled DNA fragments from each of the four reactions are denatured, separated by size by gel electrophoresis, and visualized by autoradiography or UV light.\n\nThe ddNTPs lack an OH group so the next dNTP cannot be attached, causing chain termination. +synonym: "dye terminator sequencing" EXACT [] +synonym: "Sanger sequencing" EXACT [] +is_a: ECO:0000219 ! nucleotide sequencing assay evidence +created_by: mchibucos +creation_date: 2010-11-15T04:46:31Z + +[Term] +id: ECO:0000226 +name: chromatin immunoprecipitation evidence +def: "A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence." [ECO:MCC] +comment: Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq). +synonym: "ChIP evidence" EXACT [] +xref: MI:0402 "chromatin immunoprecipitation assay" +is_a: ECO:0000085 ! immunoprecipitation evidence +created_by: mchibucos +creation_date: 2010-11-16T01:29:08Z + +[Term] +id: ECO:0000227 +name: chromatin immunoprecipitation-PCR evidence +def: "A type of chromatin immunoprecipitation evidence that uses polymerase chain reaction (PCR) where specific primers are used on the pulled-down DNA for DNA detection." [ECO:MCC, ECO:SW, PMID:18388953] +comment: ChIP-PCR is often used to validate ChIP-chip results and less frequently,ChIP-Seq results. +synonym: "ChIP-PCR evidence" EXACT [] +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: mchibucos +creation_date: 2010-11-16T05:02:00Z + +[Term] +id: ECO:0000228 +name: chromatin immunoprecipitation-qPCR evidence +def: "A type of chromatin immunoprecipitation evidence in which protein-DNA interactions are investigated at known genomic binding sites through a process of ChIP, DNA purification, and finally quantitative polymerase chain reaction (qPCR) for DNA detection." [url:https\://www.diagenode.com/applications/chip-qpcr] +synonym: "ChIP-qPCR evidence" EXACT [] +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +property_value: IAO:0000112 "To confirm the in vivo binding of FimR to pfim, and to determine the impact of Mn2+ and Fe2+ on the binding activity of FimR, chromatin immunoprecipitation (ChIP) assay-quantitative real time PCR (qPCR) with anti-FimR antibody was employed as detailed in the materials and methods. The strongest binding of FimR to pfim was detected in cells grown in the presence of 50 microM MnCl2 and 50 microM FeSO4 (Figure 5, lane IV), whereas minimal amounts of MnCl2 (0.01 microM) and FeSO4 (0.1 microM) led to the weakest binding (Figure 5, lane I)." xsd:string {xref="PMID:23823757"} +created_by: mchibucos +creation_date: 2010-11-16T05:26:47Z + +[Term] +id: ECO:0000229 +name: chromatin immunoprecipitation-seq evidence +def: "A type of chromatin immunoprecipitation evidence that uses high-throughput sequencing where immunoprecipitated DNA fragments are funnelled into a massively parallel sequencer to produce multiple short reads for locating binding sites of DNA-associated proteins." [ECO:MCC, ECO:SW, OBI:0000716, PMID:22955991] +comment: ChIP-Seq experiments often use the input as a control to eliminate biases. To obtain similar results, ChIP-chip with high-density tiling arrays can be used. +synonym: "ChIP-SEQ evidence" EXACT [OBI:0000716] +synonym: "ChIP-seq evidence" EXACT [PMID:22955991] +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: mchibucos +creation_date: 2010-11-16T05:30:41Z + +[Term] +id: ECO:0000230 +name: chromatin immunoprecipitation-chip evidence +def: "A type of chromatin immunoprecipitation evidence that uses a tiling microarray for the detection of protein-bound DNA regions where chromatin is immunoprecipitated for protein tagging and DNA is sheared from the cross-linked protein-DNA complex to be arrayed revealing the protein-bound genomic regions." [ECO:MCC, ECO:SW, PMID:18388953, PMID:19381927] +comment: A fixating agent such as formaldehyde is used to cross link proteins and DNA. Sonication is applied for DNA shearing. For transcription factor tagging, an antibody or epitope is employed. After cross-linking is reversed, the DNA is amplified and labeled with a flourophore for microarray which produces a resolution of aproximately 500 bp. +synonym: "ChIP-chip evidence" EXACT [] +synonym: "ChIP-on-chip evidence" EXACT [] +xref: MI:0225 "chromatin immunoprecipitation array" +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: mchibucos +creation_date: 2010-11-16T05:43:55Z + +[Term] +id: ECO:0000231 +name: quantitative polymerase chain reaction evidence +def: "A type of DNA detection assay evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes or fluorescent probes for detection and quantification of specific sequences in a DNA sample." [ECO:MCC, ECO:SW] +comment: Quantitative PCR enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. The dye is activated upon binding double-stranded DNA.\n\nAlthough qRT-PCR is commonly found in literature synonymous to qPCR, it is preferable not to use this synonym as it is the accepted abbreviation for reverse transcription PCR. qPCR should be used instead (ISBN 978-0-470-68386-6 page 173).\n\nqPCR can be used to quantitatively determine levels of gene expression. +synonym: "Q-PCR evidence" EXACT [] +synonym: "qPCR evidence" EXACT [] +synonym: "qRT-PCR evidence" RELATED [] +synonym: "quantitative PCR evidence" EXACT [] +synonym: "quantitative real-time PCR evidence" EXACT [] +synonym: "real time polymerase chain reaction evidence" EXACT [] +synonym: "real-time PCR evidence" EXACT [] +synonym: "real-time quantitative PCR" EXACT [] +is_a: ECO:0005519 ! DNA detection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: mchibucos +creation_date: 2010-11-16T05:57:20Z + +[Term] +id: ECO:0000232 +name: chromosome conformation-based evidence +def: "A type of experimental evidence that is based on a five-step technique of cross-linking DNA in vivo, restriction digestion, intramolecular ligation, reversing DNA cross-links, DNA processing, and quantitation that serves to analyze the spatial organization of chromatin in a cell and quantify the number of interactions between genomic loci that are nearby in 3-D space." [ECO:MCC] +comment: Chromosome conformation capture technologies are used to study the structural properties and spatial organization of chromosomes inside cells. All types of chromosome conformation capture technology involve five primary steps: cross-linking of DNA in vivo, restriction digestion, intramolecular ligation, reversing cross-linkages, and DNA enrichment and quantitation. The last step is different for each type of chromosome conformation capture technology, and may include PCR (3C), inverse PCR (4C), or ligation-mediated amplification (5C). Methods for product quantitation can include agarose gel detection or real-time quantitative PCR in the case of 3C, or high-throughput sequencing or microarray analysis for 4C and 5C. +synonym: "3C" NARROW [] +synonym: "chromosome conformation capture" NARROW [] +synonym: "chromosome conformation capture-based evidence" EXACT [] +is_a: ECO:0000006 ! experimental evidence +created_by: mchibucos +creation_date: 2010-11-17T12:27:14Z + +[Term] +id: ECO:0000233 +name: 3C evidence +def: "A type of chromosome conformation-based evidence that is derived by 1) quantifying interactions between a single pair of genomic loci and not multiple interactions and 2) performing polymerase chain reaction (PCR) in the final product quantitation step." [ECO:MCC] +comment: 3C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C differs only in the fifth step, which involves performing either standard polymerase chain reaction (PCR) or quantitative PCR (qPCR) for product quantitation. +synonym: "3C" EXACT [] +synonym: "chromosome conformation capture evidence" EXACT [] +synonym: "chromosome conformation capture-PCR evidence" NARROW [] +is_a: ECO:0000232 ! chromosome conformation-based evidence +created_by: mchibucos +creation_date: 2010-11-17T12:46:15Z + +[Term] +id: ECO:0000234 +name: 4C evidence +def: "A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray." [ECO:MCC, PMID:17033624] +comment: 4C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 4C differs only in the fifth step, which involves inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation. +synonym: "chromosome conformation capture on chip" EXACT [] +synonym: "circularized 3C" EXACT [] +synonym: "circularized chromosome conformation capture evidence" EXACT [] +is_a: ECO:0000232 ! chromosome conformation-based evidence +created_by: mchibucos +creation_date: 2010-11-19T01:20:28Z + +[Term] +id: ECO:0000235 +name: 5C evidence +def: "A type of chromosome conformation-based evidence that is derived by performing ligation-mediated amplification (LMA) prior to product quantitation." [ECO:MCC] +comment: 5C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 5C differs only in the fifth step, which involves ligation-mediated amplification (LMA) followed by product quantitation. +synonym: "5C" EXACT [] +synonym: "carbon-copy 3C" EXACT [] +synonym: "carbon-copy chromosome conformation capture evidence" EXACT [] +is_a: ECO:0000232 ! chromosome conformation-based evidence +created_by: mchibucos +creation_date: 2010-11-19T01:22:20Z + +[Term] +id: ECO:0000236 +name: chromosome conformation capture-PCR evidence +def: "A type of chromosome conformation capture evidence that is derived by performing polymerase chain reaction (PCR) in the quantitation step." [ECO:MCC] +comment: See 3C evidence. 3C evidence was initially developed using PCR. +synonym: "3C" BROAD [] +synonym: "3C-PCR" EXACT [] +is_obsolete: true +created_by: mchibucos +creation_date: 2010-11-19T03:00:47Z + +[Term] +id: ECO:0000237 +name: 3C-qPCR evidence +def: "A type of 3C evidence evidence that 1) quantifies interactions between a single pair of genomic loci (not multiple interactions) and 2) performs quantitative polymerase chain reaction (qPCR) in the quantitation step." [ECO:MCC, PMID:17641637, PMID:26025624] +synonym: "3C-quantitative PCR" EXACT [] +synonym: "3C-real-time PCR" EXACT [] +synonym: "chromosome conformation capture-qPCR evidence" EXACT [] +is_a: ECO:0000233 ! 3C evidence +created_by: mchibucos +creation_date: 2010-11-19T03:06:47Z + +[Term] +id: ECO:0000238 +name: Hi-C evidence +def: "A type of chromosome conformation-based evidence that is derived by performing immunoprecipitation enrichment of the biotin labelled ligated fragments events prior to sequencing of the library and mapping it to the genome." [ECO:MCC] +comment: Hi-C is used to study the structural properties and spatial organization of chromosomes inside cells. Hi-C is similar to other types of chromosome conformation capture technology, but involves the addition of biotinylated nucleotides during the second or fourth step and enrichment for ligated fragments using immunoprecipitation after the fourth step. +is_a: ECO:0000232 ! chromosome conformation-based evidence +created_by: mchibucos +creation_date: 2010-11-19T04:24:03Z + +[Term] +id: ECO:0000239 +name: 3C-seq evidence +def: "A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with multiplexed high-throughput (HT) sequencing." [ECO:MCC, PMID:23411633] +comment: 3C-seq is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C-seq differs only in the fifth step, which involves inverse polymerase chain reaction (PCR) on a circularized 3C library prior to product quantitation. +synonym: "3C sequencing" EXACT [] +synonym: "chromosome conformation capture sequencing evidence" RELATED [] +synonym: "circularized 3C sequencing" EXACT [] +synonym: "circularized 3C-seq" EXACT [] +is_a: ECO:0000232 ! chromosome conformation-based evidence +created_by: mchibucos +creation_date: 2010-11-30T03:22:25Z + +[Term] +id: ECO:0000240 +name: anatomical perturbation phenotypic evidence +def: "A type of experimental phenotypic evidence resulting from the disruption of a structural feature." [ECO:MCC] +synonym: "anatomical perturbation evidence" EXACT [] +is_a: ECO:0000059 ! experimental phenotypic evidence +created_by: mchibucos +creation_date: 2010-12-06T12:24:16Z + +[Term] +id: ECO:0000241 +name: environmental perturbation phenotypic evidence +def: "A type of experimental phenotypic evidence resulting from modification to the surroundings or conditions in which an organism lives or operates." [ECO:MCC] +comment: Many environmental factors are subject to modification, such as temperature, pressure, humidity, radiation, and so forth. +synonym: "environmental perturbation evidence" EXACT [] +synonym: "mechanical constraint evidence" RELATED [] +is_a: ECO:0000059 ! experimental phenotypic evidence +created_by: mchibucos +creation_date: 2010-12-06T12:33:58Z + +[Term] +id: ECO:0000242 +name: tissue ablation phenotypic evidence +def: "A type of anatomical perturbation phenotypic evidence resulting from the surgical removal of tissue or subcellular components." [ECO:MCC] +synonym: "ablated tissue evidence" EXACT [] +synonym: "tissue ablation evidence" EXACT [] +is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence +created_by: mchibucos +creation_date: 2010-12-06T12:33:58Z + +[Term] +id: ECO:0000243 +name: tissue grafting phenotypic evidence +def: "A type of anatomical perturbation phenotypic evidence resulting from the addition of tissue to an organism through a graft procedure." [ECO:MCC] +synonym: "tissue grafting evidence" EXACT [] +is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence +created_by: mchibucos +creation_date: 2010-12-06T12:33:58Z + +[Term] +id: ECO:0000244 +name: combinatorial evidence used in manual assertion +def: "A type of combinatorial analysis that is used in a manual assertion." [ECO:MCC] +comment: Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments. +synonym: "inferred from in-silico analysis" RELATED [] +is_a: ECO:0000212 ! combinatorial evidence +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +intersection_of: ECO:0000212 ! combinatorial evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: mchibucos +creation_date: 2010-12-09T02:02:12Z + +[Term] +id: ECO:0000245 +name: automatically integrated combinatorial evidence used in manual assertion +def: "A type of automatically integrated combinatorial evidence that is used in a manual assertion." [ECO:RCT] +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +synonym: "computational combinatorial evidence used in manual assertion" RELATED [] +synonym: "inferred from reviewed computational analysis" EXACT [GOECO:RCA] +synonym: "RCA" EXACT [GOECO:RCA] +xref: GOECO:RCA "inferred from reviewed computational analysis" +is_a: ECO:0000244 {is_inferred="true"} ! combinatorial evidence used in manual assertion +is_a: ECO:0007673 {is_inferred="true"} ! automatically integrated combinatorial evidence +intersection_of: ECO:0007673 ! automatically integrated combinatorial evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "RCA" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/rca-inferred-reviewed-computational-analysis xsd:string +created_by: mchibucos +creation_date: 2010-12-09T02:37:54Z + +[Term] +id: ECO:0000246 +name: computational combinatorial evidence used in automatic assertion +def: "A type of computational combinatorial analysis that is used in an automatic assertion." [ECO:MCC] +comment: Merged with ECO:0000053. +is_obsolete: true +created_by: mchibucos +creation_date: 2010-12-09T02:37:54Z + +[Term] +id: ECO:0000247 +name: sequence alignment evidence used in manual assertion +def: "A type of sequence alignment evidence that is used in a manual assertion." [ECO:RCT] +subset: valid_with_gene +subset: valid_with_protein +synonym: "inferred from sequence alignment" EXACT [GOECO:ISA] +synonym: "ISA" EXACT [GOECO:ISA] +xref: GOECO:ISA "inferred from sequence alignment" +is_a: ECO:0000200 ! sequence alignment evidence +is_a: ECO:0000250 {is_inferred="true"} ! sequence similarity evidence used in manual assertion +intersection_of: ECO:0000200 ! sequence alignment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISA" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/isa-inferred-sequence-alignment xsd:string +created_by: mchibucos +creation_date: 2010-12-09T05:12:27Z + +[Term] +id: ECO:0000248 +name: sequence alignment evidence used in automatic assertion +def: "A type of sequence alignment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000200 ! sequence alignment evidence +is_a: ECO:0000249 {is_inferred="true"} ! sequence similarity evidence used in automatic assertion +intersection_of: ECO:0000200 ! sequence alignment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-09T05:12:27Z + +[Term] +id: ECO:0000249 +name: sequence similarity evidence used in automatic assertion +def: "A type of sequence similarity evidence that is used in an automatic assertion." [ECO:RCT] +xref: GO_REF:0000107 "Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl" +is_a: ECO:0000044 ! sequence similarity evidence +is_a: ECO:0000251 {is_inferred="true"} ! similarity evidence used in automatic assertion +is_a: ECO:0007669 {is_inferred="true"} ! computational evidence used in automatic assertion +intersection_of: ECO:0000044 ! sequence similarity evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-09T05:22:30Z + +[Term] +id: ECO:0000250 +name: sequence similarity evidence used in manual assertion +def: "A type of sequence similarity evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +synonym: "inferred from sequence or structural similarity" EXACT [GOECO:ISS] +synonym: "ISS" EXACT [GOECO:ISS] +xref: GOECO:ISS "inferred from sequence or structural similarity" +is_a: ECO:0000044 ! sequence similarity evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +is_a: ECO:0007668 {is_inferred="true"} ! computational evidence used in manual assertion +intersection_of: ECO:0000044 ! sequence similarity evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISS" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/iss-inferred-sequence-or-structural-similarity xsd:string +created_by: mchibucos +creation_date: 2010-12-09T05:22:30Z + +[Term] +id: ECO:0000251 +name: similarity evidence used in automatic assertion +def: "A type of similarity evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "inferred from similarity" RELATED [] +synonym: "IS" BROAD [] +is_a: ECO:0000041 ! similarity evidence +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +intersection_of: ECO:0000041 ! similarity evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-09T05:28:12Z + +[Term] +id: ECO:0000252 +name: similarity evidence used in manual assertion +def: "A type of similarity evidence that is used in a manual assertion." [ECO:RCT] +subset: go_groupings +synonym: "inferred from similarity" RELATED [] +synonym: "IS" BROAD [] +is_a: ECO:0000041 ! similarity evidence +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +intersection_of: ECO:0000041 ! similarity evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: mchibucos +creation_date: 2010-12-09T05:28:12Z + +[Term] +id: ECO:0000253 +name: genetic similarity evidence used in manual assertion +def: "A type of genetic similarity evidence that is used in a manual assertion." [ECO:RCT] +synonym: "IGTS" BROAD [] +synonym: "inferred from genetic similarity" RELATED [] +is_a: ECO:0000051 ! genetic similarity evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +intersection_of: ECO:0000051 ! genetic similarity evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: mchibucos +creation_date: 2010-12-10T01:42:44Z + +[Term] +id: ECO:0000254 +name: genetic similarity evidence used in automatic assertion +def: "A type of genetic similarity evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "IGTS" BROAD [] +synonym: "inferred from genetic similarity" RELATED [] +is_a: ECO:0000051 ! genetic similarity evidence +is_a: ECO:0000251 {is_inferred="true"} ! similarity evidence used in automatic assertion +intersection_of: ECO:0000051 ! genetic similarity evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T01:42:44Z + +[Term] +id: ECO:0000255 +name: match to sequence model evidence used in manual assertion +def: "A type of match to sequence model evidence that is used in a manual assertion." [ECO:RCT] +synonym: "inferred from sequence model" EXACT [GOECO:ISM] +synonym: "ISM" EXACT [GOECO:ISM] +xref: GO_REF:0000011 "Hidden Markov Models (TIGR)" +xref: GOECO:ISM "inferred from sequence model" +is_a: ECO:0000202 ! match to sequence model evidence +is_a: ECO:0000250 {is_inferred="true"} ! sequence similarity evidence used in manual assertion +intersection_of: ECO:0000202 ! match to sequence model evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISM" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ism-inferred-sequence-model xsd:string +created_by: mchibucos +creation_date: 2010-12-10T02:26:35Z + +[Term] +id: ECO:0000256 +name: match to sequence model evidence used in automatic assertion +def: "A type of match to sequence model evidence that is used in an automatic assertion." [ECO:RCT] +xref: GO_REF:0000002 "Gene Ontology annotation through association of InterPro records with GO terms." +is_a: ECO:0000202 ! match to sequence model evidence +is_a: ECO:0000249 {is_inferred="true"} ! sequence similarity evidence used in automatic assertion +intersection_of: ECO:0000202 ! match to sequence model evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T02:26:35Z + +[Term] +id: ECO:0000257 +name: motif similarity evidence used in manual assertion +def: "A type of motif similarity evidence that is used in a manual assertion." [ECO:RCT] +synonym: "recognized domains" RELATED [] +is_a: ECO:0000028 ! motif similarity evidence +is_a: ECO:0000255 {is_inferred="true"} ! match to sequence model evidence used in manual assertion +intersection_of: ECO:0000028 ! motif similarity evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T02:44:34Z + +[Term] +id: ECO:0000258 +name: motif similarity evidence used in automatic assertion +def: "A type of motif similarity evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "recognized domains" RELATED [] +is_a: ECO:0000028 ! motif similarity evidence +is_a: ECO:0000256 {is_inferred="true"} ! match to sequence model evidence used in automatic assertion +intersection_of: ECO:0000028 ! motif similarity evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T02:44:34Z + +[Term] +id: ECO:0000259 +name: match to InterPro member signature evidence used in automatic assertion +def: "A type of match to InterPro member signature evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000029 ! match to InterPro member signature evidence +is_a: ECO:0000256 {is_inferred="true"} ! match to sequence model evidence used in automatic assertion +intersection_of: ECO:0000029 ! match to InterPro member signature evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T02:54:51Z + +[Term] +id: ECO:0000260 +name: match to InterPro member signature evidence used in manual assertion +def: "A type of match to InterPro member signature evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000029 ! match to InterPro member signature evidence +is_a: ECO:0000255 {is_inferred="true"} ! match to sequence model evidence used in manual assertion +intersection_of: ECO:0000029 ! match to InterPro member signature evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T02:54:51Z + +[Term] +id: ECO:0000261 +name: targeting sequence prediction evidence used in automatic assertion +def: "A type of targeting sequence prediction that is used in an automatic assertion." [ECO:RCT] +synonym: "targeting sequence prediction" RELATED [] +is_a: ECO:0000081 ! targeting sequence prediction evidence +is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion +intersection_of: ECO:0000081 ! targeting sequence prediction evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T03:02:49Z + +[Term] +id: ECO:0000262 +name: targeting sequence prediction evidence used in manual assertion +def: "A type of targeting sequence prediction that is used in a manual assertion." [ECO:RCT] +synonym: "targeting sequence prediction" RELATED [] +is_a: ECO:0000081 ! targeting sequence prediction evidence +is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion +intersection_of: ECO:0000081 ! targeting sequence prediction evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T03:02:49Z + +[Term] +id: ECO:0000263 +name: transmembrane domain prediction evidence used in automatic assertion +def: "A type of transmembrane domain prediction that is used in an automatic assertion." [ECO:RCT] +synonym: "transmembrane domain prediction" RELATED [] +is_a: ECO:0000083 ! transmembrane domain prediction evidence +is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion +intersection_of: ECO:0000083 ! transmembrane domain prediction evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T03:28:35Z + +[Term] +id: ECO:0000264 +name: transmembrane domain prediction evidence used in manual assertion +def: "A type of transmembrane domain prediction that is used in a manual assertion." [ECO:RCT] +synonym: "transmembrane domain prediction" RELATED [] +is_a: ECO:0000083 ! transmembrane domain prediction evidence +is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion +intersection_of: ECO:0000083 ! transmembrane domain prediction evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T03:28:35Z + +[Term] +id: ECO:0000265 +name: sequence orthology evidence used in automatic assertion +def: "A type of sequence orthology evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "Ortholog evidence" RELATED [] +synonym: "ortholog evidence" RELATED [] +xref: GO_REF:0000035 "Automatic transfer of experimentally verified manual GO annotation data to plant orthologs using Ensembl Compara" +is_a: ECO:0000201 ! sequence orthology evidence +is_a: ECO:0000249 {is_inferred="true"} ! sequence similarity evidence used in automatic assertion +intersection_of: ECO:0000201 ! sequence orthology evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2010-12-10T03:39:41Z + +[Term] +id: ECO:0000266 +name: sequence orthology evidence used in manual assertion +def: "A type of sequence orthology evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +synonym: "inferred from sequence orthology" EXACT [] {name="GOECO:ISO"} +synonym: "ISO" EXACT [GOECO:ISO] +synonym: "Ortholog evidence" RELATED [] +synonym: "ortholog evidence" RELATED [] +xref: GOECO:ISO "inferred from sequence orthology" +is_a: ECO:0000201 ! sequence orthology evidence +is_a: ECO:0000250 {is_inferred="true"} ! sequence similarity evidence used in manual assertion +intersection_of: ECO:0000201 ! sequence orthology evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISO" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/iso-inferred-sequence-orthology xsd:string +created_by: mchibucos +creation_date: 2010-12-10T03:39:41Z + +[Term] +id: ECO:0000267 +name: enzyme-linked immunoabsorbent assay evidence +def: "A type of protein detection assay evidence where an antigen is bound to a substrate, and an antibody directly or indirectly linked to an enzyme is utilized to determine the amount of bound antigen which is visualized with a color change in the substrate." [ECO:MCC, ECO:SW, PMID:23949770] +comment: ELISA is sometimes used in the study of transcriptional regulation to identify a produced protein, especially secreted protein. +synonym: "ELISA evidence" EXACT [] +synonym: "enzyme-linked immunosorbent assay" NARROW [] +xref: MI:0411 "enzyme linked immunosorbent assay" +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: mchibucos +creation_date: 2010-12-14T01:57:13Z + +[Term] +id: ECO:0000268 +name: flow cytometry evidence +def: "A type of direct assay evidence in which cells or particles are characterized by passing them through a light source and monitoring the resultant light scatter or fluorochrome excitation." [ECO:MCC] +synonym: "FCM" EXACT [] +is_a: ECO:0000002 ! direct assay evidence +created_by: mchibucos +creation_date: 2010-12-14T02:07:08Z + +[Term] +id: ECO:0000269 +name: experimental evidence used in manual assertion +def: "A type of experimental evidence that is used in a manual assertion." [ECO:MCC] +synonym: "EXP" EXACT [GOECO:EXP] +synonym: "inferred from experiment" EXACT [GOECO:EXP] +xref: GOECO:EXP "inferred from experiment" +is_a: ECO:0000006 ! experimental evidence +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +intersection_of: ECO:0000006 ! experimental evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/exp-inferred-experiment xsd:string +created_by: mchibucos +creation_date: 2010-12-20T05:36:49Z + +[Term] +id: ECO:0000270 +name: expression pattern evidence used in manual assertion +def: "A type of expression pattern evidence that is used in a manual assertion." [ECO:MCC] +synonym: "IEP" EXACT [GOECO:IEP] +synonym: "inferred from expression pattern" EXACT [GOECO:IEP] +xref: GOECO:IEP "inferred from expression pattern" +is_a: ECO:0000008 ! expression pattern evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000008 ! expression pattern evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/iep-inferred-expression-pattern xsd:string +created_by: mchibucos +creation_date: 2010-12-20T05:46:39Z + +[Term] +id: ECO:0000271 +name: array experiment evidence used in manual assertion +comment: This general 'array experiment' term should not be used - replace with the specific type of array (e.g. ECO:0000273, ECO:0000276, ECO:0000285, etc.) +is_obsolete: true +created_by: mchibucos +creation_date: 2010-12-20T05:51:58Z + +[Term] +id: ECO:0000272 +name: Affymetrix GeneChip evidence used in manual assertion +def: "A type of Affymetrix GeneChip evidence that is used in a manual assertion." [ECO:MCC] +synonym: "Affymetrix array experiment evidence" BROAD [] +is_a: ECO:0000101 ! Affymetrix GeneChip evidence +is_a: ECO:0000276 {is_inferred="true"} ! cRNA to DNA expression microarray evidence used in manual assertion +intersection_of: ECO:0000101 ! Affymetrix GeneChip evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T05:53:36Z + +[Term] +id: ECO:0000273 +name: cDNA to DNA expression microarray evidence used in manual assertion +alt_id: ECO:0001178 +alt_id: ECO:0005525 +def: "A type of cDNA to DNA expression microarray evidence that is used in a manual assertion." [ECO:MCC] +synonym: "DNA microarray|RNA microarray" RELATED [] +is_a: ECO:0000097 ! cDNA to DNA expression microarray evidence +is_a: ECO:0000275 {is_inferred="true"} ! expression microarray evidence used in manual assertion +intersection_of: ECO:0000097 ! cDNA to DNA expression microarray evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T05:55:49Z + +[Term] +id: ECO:0000274 +name: differential methylation hybridization evidence used in manual assertion +def: "A type of differential methylation hybridization evidence that is used in a manual assertion." [ECO:RCT] +synonym: "CpG island microarray evidence" BROAD [] +synonym: "CpG island microarray evidence used in manual assertion" EXACT [] +is_a: ECO:0000069 ! differential methylation hybridization evidence +is_a: ECO:0001198 {is_inferred="true"} ! in vitro methylation assay evidence used in manual assertion +intersection_of: ECO:0000069 ! differential methylation hybridization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T05:58:44Z + +[Term] +id: ECO:0000275 +name: expression microarray evidence used in manual assertion +def: "A type of expression microarray evidence that is used in a manual assertion." [ECO:MCC] +synonym: "differential gene expression evidence from microarray experiment" RELATED [] +is_a: ECO:0000058 ! expression microarray evidence +is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion +intersection_of: ECO:0000058 ! expression microarray evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:01:26Z + +[Term] +id: ECO:0000276 +name: cRNA to DNA expression microarray evidence used in manual assertion +def: "A type of cRNA to DNA expression microarray evidence that is used in a manual assertion." [ECO:RCT] +synonym: "genomic microarray evidence" BROAD [] +synonym: "genomic microarray evidence used in manual assertion" EXACT [] +is_a: ECO:0000062 ! cRNA to DNA expression microarray evidence +is_a: ECO:0000275 {is_inferred="true"} ! expression microarray evidence used in manual assertion +intersection_of: ECO:0000062 ! cRNA to DNA expression microarray evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:03:28Z + +[Term] +id: ECO:0000277 +name: Nimblegen array evidence used in manual assertion +def: "A type of evidence arising from a Nimblegen array experiment that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000105 ! Nimblegen array evidence +is_a: ECO:0000273 {is_inferred="true"} ! cDNA to DNA expression microarray evidence used in manual assertion +intersection_of: ECO:0000105 ! Nimblegen array evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:05:20Z + +[Term] +id: ECO:0000278 +name: array-based sequence capture evidence used in manual assertion +def: "A type of array-based sequence capture evidence that is used in a manual assertion." [ECO:MCC] +synonym: "DNA microarray" BROAD [] +synonym: "oligonucleotide microarray evidence" BROAD [] +synonym: "oligonucleotide microarray evidence used in manual assertion" BROAD [] +synonym: "single nucleotide polymorphism array evidence" RELATED [] +synonym: "SNP array evidence" RELATED [] +is_a: ECO:0000093 ! array-based sequence capture evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000093 ! array-based sequence capture evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:09:45Z + +[Term] +id: ECO:0000279 +name: qualitative western immunoblotting evidence used in manual assertion +alt_id: ECO:0007183 +def: "A type of qualitative western immunoblotting evidence that is used in a manual assertion." [ECO:MCC] +synonym: "protein expression level evidence based on western blot used in manual assertion" EXACT [] +synonym: "protein immunoblot" RELATED [] +synonym: "protein levels (e.g. Western blots)" RELATED [] +synonym: "Western blot expression analysis" RELATED [] +is_a: ECO:0000112 ! qualitative western immunoblotting evidence +is_a: ECO:0000284 {is_inferred="true"} ! protein expression evidence used in manual assertion +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000112 ! qualitative western immunoblotting evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:20:01Z + +[Term] +id: ECO:0000281 +name: expression library screen evidence used in manual assertion +def: "A type of expression library screen evidence that is used in a manual assertion." [ECO:MCC] +synonym: "expression library screening" RELATED [] +is_a: ECO:0000114 ! expression library screen evidence +is_a: ECO:0000284 {is_inferred="true"} ! protein expression evidence used in manual assertion +intersection_of: ECO:0000114 ! expression library screen evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:24:05Z + +[Term] +id: ECO:0000282 +name: heterologous protein expression evidence used in manual assertion +def: "A type of heterologous protein expression analysis that is used in a manual assertion." [ECO:MCC] +synonym: "protein expression in heterologous system" RELATED [] +is_a: ECO:0000154 ! heterologous protein expression evidence +is_a: ECO:0000284 {is_inferred="true"} ! protein expression evidence used in manual assertion +intersection_of: ECO:0000154 ! heterologous protein expression evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:27:04Z + +[Term] +id: ECO:0000283 +name: spatial pattern of protein expression evidence used in manual assertion +def: "A type of protein expression spatial pattern analysis that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000045 ! spatial pattern of protein expression evidence +is_a: ECO:0000284 {is_inferred="true"} ! protein expression evidence used in manual assertion +intersection_of: ECO:0000045 ! spatial pattern of protein expression evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:31:44Z + +[Term] +id: ECO:0000284 +name: protein expression evidence used in manual assertion +alt_id: ECO:0000280 +def: "A type of protein expression analysis that is used in a manual assertion." [ECO:MCC] +synonym: "protein expression level evidence used in manual assertion" EXACT [] +is_a: ECO:0000010 ! protein expression evidence +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +intersection_of: ECO:0000010 ! protein expression evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:33:46Z + +[Term] +id: ECO:0000285 +name: DNA to cDNA expression microarray evidence used in manual assertion +def: "A type of DNA to cDNA expression microarray evidence that is used in a manual assertion." [ECO:MCC] +synonym: "microarray RNA expression level evidence" BROAD [] +synonym: "REM evidence" BROAD [] +synonym: "RNA expression microarray evidence" BROAD [] +synonym: "transcript levels (e.g. microarray data)" RELATED [] +is_a: ECO:0000104 ! DNA to cDNA expression microarray evidence +is_a: ECO:0000275 {is_inferred="true"} ! expression microarray evidence used in manual assertion +intersection_of: ECO:0000104 ! DNA to cDNA expression microarray evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:41:33Z + +[Term] +id: ECO:0000287 +name: differential hybridization evidence used in manual assertion +def: "A type of differential hybridization experiment that is used in a manual assertion." [ECO:MCC] +synonym: "differential hybridization" RELATED [] +is_a: ECO:0000116 ! differential hybridization evidence +is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion +intersection_of: ECO:0000116 ! differential hybridization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:55:14Z + +[Term] +id: ECO:0000288 +name: RNA protection assay evidence used in manual assertion +def: "A type of RNA protection assay that is used in a manual assertion." [ECO:MCC] +synonym: "ribonuclease protection assay" BROAD [] +synonym: "RNA protection assay|RPA" RELATED [] +synonym: "RNAse protection assay" RELATED [] +is_a: ECO:0000110 ! RNA protection assay evidence +is_a: ECO:0001240 {is_inferred="true"} ! nuclease protection assay evidence used in manual assertion +intersection_of: ECO:0000110 ! RNA protection assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:57:33Z + +[Term] +id: ECO:0000289 +name: spatial pattern of transcript expression evidence used in manual assertion +def: "A type of spatial pattern of transcript expression analysis that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000047 ! spatial pattern of transcript expression evidence +is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion +intersection_of: ECO:0000047 ! spatial pattern of transcript expression evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T06:59:18Z + +[Term] +id: ECO:0000290 +name: subtractive hybridization evidence used in manual assertion +def: "A type of subtractive hybridization experiment that is used in a manual assertion." [ECO:MCC] +synonym: "subtractive hybridization" RELATED [] +is_a: ECO:0000118 ! subtractive hybridization evidence +is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion +intersection_of: ECO:0000118 ! subtractive hybridization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T07:00:48Z + +[Term] +id: ECO:0000291 +name: transcript expression evidence used in manual assertion +alt_id: ECO:0000286 +def: "A type of transcript expression evidence that is used in a manual assertion." [ECO:MCC] +synonym: "transcript expression level evidence" BROAD [] +synonym: "transcriptomics evidence used in manual assertion" EXACT [] +is_a: ECO:0000009 ! transcript expression evidence +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +intersection_of: ECO:0000009 ! transcript expression evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2010-12-20T07:02:08Z + +[Term] +id: ECO:0000292 +name: morpholino experiment evidence +def: "A type of mutant phenotype evidence that uses anti-sense by introducing morpholino oligonucleotides into the cytosol of a cell." [ECO:MCC] +comment: Morpholino oligonucleotides modify gene expression by blocking access of other molecules to specific, approximately 25-base-long regions of the base-pairing surfaces of ribonucleic acid (RNA). To achieve this anti-sense knockdown technique, delivery of the morpholino can be achieved via microinjection, electroporation, or another method. +synonym: "anti-sense" BROAD [] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: mchibucos +creation_date: 2011-01-10T03:36:25Z + +[Term] +id: ECO:0000293 +name: systematic evolution of ligands by exponential amplification evidence +def: "A type of evidence arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand." [ECO:MCC, url:http\://en.wikipedia.org/wiki/Systematic_Evolution_of_Ligands_by_Exponential_Enrichment] +comment: SELEX begins with the synthesis of a large oligonucleotide library, either single-stranded DNA or RNA, which consists of random fixed-length sequences flanked by constant 5' and 3' ends that serve as primers. The library is exposed to a target ligand, typically a protein or small organic compound. Unbound sequences are removed, typically with affinity chromatography. Bound sequences are eluted and the nucleic acid is extracted, followed by PCR amplification (RNA is first reverse transcribed). PCR product is converted to single stranded (DNA) or transcribed (RNA). Oligonucleotides are bound to the ligand again and the process is repeated with increasing elution stringency. After several rounds of the process, recovered oligonucleotides are sequenced and analyzed to reveal the binding specificity of the protein. +synonym: "in vitro evolution evidence" RELATED [] +synonym: "in vitro selection evidence" EXACT [] +synonym: "SELEX evidence" EXACT [] +xref: MI:0657 "systematic evolution of ligands by exponential enrichment" +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000112 "A total of 160 SELEX fragments were cloned and sequenced, which were classified into 29 CRP-binding sequences, including 13 previously identified loci and 16 newly identified sites (Table S2). When the CRP-binding sequences are located upstream or near promoters of the flanking genes, these genes are predicted to be under the control of CRP." xsd:string {xref="PMID:21673794"} +created_by: mchibucos +creation_date: 2011-01-10T04:23:50Z + +[Term] +id: ECO:0000294 +name: bacterial one-hybrid evidence +def: "A type of bait-prey hybrid interaction evidence that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey)." [ECO:MCC] +comment: Bacterial one-hybrid assay is a method for screening a library of candidate proteins (prey plasmid) for the ability to bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to reporter genes (bait plasmid). Transformation of a bacterial host with two different plasmids is required: One is designed to express the "bait". The other plasmid contains a "prey" which, if bound to by the chimeric fusion product, drives expression of downstream reporter genes. +synonym: "B1H evidence" EXACT [] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence +property_value: IAO:0000112 "Bacterial one-hybrid assays confirmed the interaction between MtrA and the regulatory sequence of the dnaA initiator gene." xsd:string {xref="PMID:20843371"} +created_by: mchibucos +creation_date: 2011-01-10T06:16:20Z + +[Term] +id: ECO:0000295 +name: RNA-sequencing evidence +alt_id: ECO:0000357 +def: "A type of transcript evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC] +comment: Total RNA is isolated from cells and mRNA is purified by targeting the 3' polyadenylated (poly(A)) tail with poly(T) oligos covalently attached to a substrate. Next, cDNA fragments are generated either by hydrolysis of RNA into 200-300 base oligonucleotides, preceded by reverse transcription, or by reverse transcription initiated by random primers. The cDNA second strand is synthesized, fragments are adapter ligated and high-throughput sequencing is performed by Roche 454, Illumina, ABI-SOLiD, or another HT sequencing technology. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed. +synonym: "differential gene expression evidence from RNA-seq experiment" NARROW [] +synonym: "RNA sequencing" RELATED [] +synonym: "RNA-seq evidence" EXACT [] +synonym: "RNAseq evidence" EXACT [] +synonym: "whole transcriptome shotgun sequencing" EXACT [PMID:18611170] +synonym: "WTSS" EXACT [] +is_a: ECO:0000009 ! transcript expression evidence +created_by: mchibucos +creation_date: 2011-01-11T12:54:50Z + +[Term] +id: ECO:0000298 +name: cleavage arrested development evidence +def: "A type of experimental phenotypic evidence where embryonic development proceeds although cytokinesis is blocked." [ECO:MCC] +comment: In the embryos of some organisms, inhibition of cytokinesis blocks neither the cell cycle nor the unfolding of the developmental program. As such, embryonic morphology can be frozen, allowing analysis of the developmental fate of each cell present at the time of cleavage arrest. +is_a: ECO:0000059 ! experimental phenotypic evidence +created_by: mchibucos +creation_date: 2011-01-11T03:28:49Z + +[Term] +id: ECO:0000299 +name: cytochalasin experiment evidence +def: "A type of cleavage arrested development that arises after treatment with cytochalasin." [ECO:MCC] +comment: Cytochalasin inhibits cytokinesis by blocking the formation of contractile microfilaments. +is_a: ECO:0000298 ! cleavage arrested development evidence +created_by: mchibucos +creation_date: 2011-01-11T03:30:29Z + +[Term] +id: ECO:0000300 +name: green fluorescent protein immunolocalization evidence +def: "A type of immunolocalization evidence data that was generated using green fluorescent protein (GFP) as a marker." [ECO:MCC] +comment: This term describes immunolocalization of the protein; to describe transcriptional activation, use the term "localization of green fluorescent protein transcript". +synonym: "GFP immunolocalization evidence" EXACT [] +is_a: ECO:0000087 ! immunolocalization evidence +created_by: mchibucos +creation_date: 2011-01-11T03:48:50Z + +[Term] +id: ECO:0000301 +name: beta-galactosidase protein immunolocalization evidence +def: "A type of immunolocalization evidence that was generated using LacZ protein as a marker." [ECO:MCC] +comment: This term describes immunolocalization of the protein; to describe transcriptional activation, use the term "localization of LacZ transcript". +synonym: "LacZ protein immunolocalization evidence" EXACT [] +is_a: ECO:0000087 ! immunolocalization evidence +created_by: mchibucos +creation_date: 2011-01-11T04:06:40Z + +[Term] +id: ECO:0000302 +name: author statement used in manual assertion +alt_id: ECO:0007764 +def: "A type of author statement that is used in a manual assertion." [ECO:MCC] +synonym: "author inference used in manual assertion" EXACT [] +is_a: ECO:0000204 ! author statement +is_a: ECO:0006155 {is_inferred="true"} ! documented statement evidence used in manual assertion +intersection_of: ECO:0000204 ! author statement +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: mchibucos +creation_date: 2011-01-20T02:26:47Z + +[Term] +id: ECO:0000303 +name: author statement without traceable support used in manual assertion +def: "A type of author statement without traceable support that is used in a manual assertion." [ECO:MCC] +synonym: "NAS" EXACT [GOECO:NAS] +synonym: "non-traceable author statement" RELATED [GOECO:NAS] +xref: GOECO:NAS "non-traceable author statement" +is_a: ECO:0000034 ! author statement without traceable support +is_a: ECO:0000302 {is_inferred="true"} ! author statement used in manual assertion +intersection_of: ECO:0000034 ! author statement without traceable support +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "NAS" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/nas-non-traceable-author-statement xsd:string +created_by: mchibucos +creation_date: 2011-01-20T02:29:50Z + +[Term] +id: ECO:0000304 +name: author statement supported by traceable reference used in manual assertion +def: "A type of author statement supported by traceable reference that is used in a manual assertion." [ECO:MCC] +synonym: "TAS" EXACT [GOECO:TAS] +synonym: "traceable author statement" RELATED [GOECO:TAS] +xref: GOECO:TAS "traceable author statement" +is_a: ECO:0000033 ! author statement supported by traceable reference +is_a: ECO:0000302 {is_inferred="true"} ! author statement used in manual assertion +intersection_of: ECO:0000033 ! author statement supported by traceable reference +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "TAS" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/tas-traceable-author-statement xsd:string +created_by: mchibucos +creation_date: 2011-01-20T02:31:03Z + +[Term] +id: ECO:0000305 +name: curator inference used in manual assertion +def: "A type of curator inference that is used in a manual assertion." [ECO:MCC] +subset: valid_with_biological_process +subset: valid_with_cellular_component +subset: valid_with_molecular_function +synonym: "IC" EXACT [GOECO:IC] +synonym: "inferred by curator" EXACT [GOECO:IC] +xref: GOECO:IC "inferred by curator" +is_a: ECO:0000205 ! curator inference +is_a: ECO:0007751 {is_inferred="true"} ! inferential evidence used in manual assertion +intersection_of: ECO:0000205 ! curator inference +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ic-inferred-curator xsd:string +created_by: mchibucos +creation_date: 2011-01-20T02:33:32Z + +[Term] +id: ECO:0000306 +name: inference from background scientific knowledge used in manual assertion +def: "A type of inference from background scientific knowledge that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000001 ! inference from background scientific knowledge +is_a: ECO:0000305 {is_inferred="true"} ! curator inference used in manual assertion +intersection_of: ECO:0000001 ! inference from background scientific knowledge +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: mchibucos +creation_date: 2011-01-20T02:35:11Z + +[Term] +id: ECO:0000307 +name: no evidence data found used in manual assertion +def: "An inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources that is used in an manual assertion." [ECO:MCC] +synonym: "ND" EXACT [GOECO:ND] +synonym: "no biological data available" EXACT [GOECO:ND] +xref: GOECO:ND "no biological data available" +is_a: ECO:0000035 ! no evidence data found +is_a: ECO:0000305 {is_inferred="true"} ! curator inference used in manual assertion +intersection_of: ECO:0000035 ! no evidence data found +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ND" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/nd-no-biological-data-available xsd:string +created_by: mchibucos +creation_date: 2011-01-20T02:43:18Z + +[Term] +id: ECO:0000308 +name: biological aspect of ancestor evidence +def: "A type of phylogenetic evidence whereby an aspect of a descendant gene is inferred through the characterization of an aspect of an ancestral gene." [ECO:MCC] +comment: First, a characteristic of the most recent common ancestor (MRCA) is inferred from experimental evidence in descendants; this is followed by inferring a characteristic of a descendant of the MRCA. This type of evidence can be used in support of both positive and "not" GO annotations. +is_a: ECO:0000080 ! phylogenetic evidence +created_by: mchibucos +creation_date: 2011-02-04T05:41:26Z + +[Term] +id: ECO:0000309 +name: cap analysis of gene expression evidence +def: "A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by a cap-trapper system." [ECO:MCC] +comment: Complementary DNA (cDNA) is synthesized from microgram quantities of mRNA using first-strand cDNA primer (oligo dT12-18) and reverse transcriptase in the presence of trehalose and sorbitol, followed by selection of full-length cDNA with biotinylated cap-trapper. Linkers are attached to the 5' ends of full-length enriched cDNAs to introduce a recognition site for the restriction endonuclease MmeI, which creates a two base overhang. After amplification, sequencing tags are concatenated for high-throughput sequencing. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed. +synonym: "CAGE" EXACT [PMID:16489339] +is_a: ECO:0000009 ! transcript expression evidence +created_by: mchibucos +creation_date: 2011-02-17T01:33:09Z + +[Term] +id: ECO:0000310 +name: nano-cap analysis of gene expression evidence +def: "A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by reverse transcriptase template switching." [ECO:MCC] +comment: NanoCAGE captures the 5' ends of molecules by template switching. When polymerizing the cDNA of a capped mRNA, the reverse transcriptase adds extra cytosines that are complementary to the cap. Each 5' full length cDNAs is extended upon hybridization of the riboguanosine-tailed template switching oligonucleotides to these extra cytosines. In semisuppressive PCR, the short templates fold intramolecularly and prevent the binding of primers, which precludes amplification; longer molecules are less likely to fold and are thus amplified. Templates derived from reaction artifacts form stable homoduplexes, also precluding amplification. After template switching, semisuppressive PCR, and EcoP151 cleavage, 25-bp tags are ligated to bar code-containing oligonucleotide adaptors. After PCR amplification, the nanoCAGE tags are sequenced by synthesis. NanoCAGE requires as little as 10 nanograms of total RNA. +synonym: "nanoCAGE" RELATED [PMID:20543846] +is_a: ECO:0000009 ! transcript expression evidence +created_by: mchibucos +creation_date: 2011-02-17T01:33:34Z + +[Term] +id: ECO:0000311 +name: imported information +def: "A type of documented statement evidence that is based on work performed by a person or group prior to a use by a different person or group." [ECO:MCC] +is_a: ECO:0006151 ! documented statement evidence +created_by: mchibucos +creation_date: 2011-03-02T05:24:13Z + +[Term] +id: ECO:0000312 +name: imported information used in manual assertion +def: "A type of imported information that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000311 ! imported information +is_a: ECO:0006155 {is_inferred="true"} ! documented statement evidence used in manual assertion +intersection_of: ECO:0000311 ! imported information +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: mchibucos +creation_date: 2011-03-02T05:29:44Z + +[Term] +id: ECO:0000313 +name: imported information used in automatic assertion +def: "A type of imported information that is used in an automatic assertion." [ECO:MCC] +is_a: ECO:0000311 ! imported information +is_a: ECO:0006156 {is_inferred="true"} ! documented statement evidence used in automatic assertion +intersection_of: ECO:0000311 ! imported information +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2011-03-02T05:33:43Z + +[Term] +id: ECO:0000314 +name: direct assay evidence used in manual assertion +def: "A type of direct assay evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_chemical_entity +synonym: "IDA" EXACT [GOECO:IDA] +synonym: "inferred from direct assay" EXACT [GOECO:IDA] +xref: GOECO:IDA "inferred from direct assay" +is_a: ECO:0000002 ! direct assay evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000002 ! direct assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ida-inferred-direct-assay xsd:string +created_by: mchibucos +creation_date: 2011-10-28T04:59:31Z + +[Term] +id: ECO:0000315 +name: mutant phenotype evidence used in manual assertion +def: "A type of mutant phenotype evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_chemical_entity +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +synonym: "IMP" EXACT [GOECO:IMP] +synonym: "inferred from mutant phenotype" EXACT [GOECO:IMP] +xref: GOECO:IMP "inferred from mutant phenotype" +is_a: ECO:0000015 ! mutant phenotype evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0000015 ! mutant phenotype evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/imp-inferred-mutant-phenotype xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:12:49Z + +[Term] +id: ECO:0000316 +name: genetic interaction evidence used in manual assertion +def: "A type of genetic interaction experiment evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +synonym: "IGI" EXACT [GOECO:IGI] +synonym: "inferred from genetic interaction" EXACT [GOECO:IGI] +xref: GOECO:IGI "inferred from genetic interaction" +is_a: ECO:0000011 ! genetic interaction evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0000011 ! genetic interaction evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IGI" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/igi-inferred-genetic-interaction xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:14:42Z + +[Term] +id: ECO:0000317 +name: genomic context evidence used in manual assertion +def: "A type of genomic context evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +synonym: "IGC" EXACT [GOECO:IGC] +synonym: "inferred from genomic context" EXACT [GOECO:IGC] +xref: GOECO:IGC "inferred from genomic context" +is_a: ECO:0000177 ! genomic context evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +intersection_of: ECO:0000177 ! genomic context evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IGC" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/igc-inferred-genomic-context xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:22:53Z + +[Term] +id: ECO:0000318 +name: biological aspect of ancestor evidence used in manual assertion +def: "A type of biological aspect of ancestor evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +synonym: "IBA" EXACT [GOECO:IBA] +synonym: "inferred from biological aspect of ancestor" EXACT [GOECO:IBA] +xref: GOECO:IBA "inferred from biological aspect of ancestor" +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +is_a: ECO:0000308 ! biological aspect of ancestor evidence +intersection_of: ECO:0000308 ! biological aspect of ancestor evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IBA" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/iba-inferred-biological-aspect-ancestor xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:23:51Z + +[Term] +id: ECO:0000319 +name: biological aspect of descendant evidence used in manual assertion +def: "A type of biological aspect of descendant evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +synonym: "IBD" EXACT [GOECO:IBD] +synonym: "inferred from biological aspect of descendant" EXACT [GOECO:IBD] +xref: GOECO:IBD "inferred from biological aspect of descendant" +is_a: ECO:0000214 ! biological aspect of descendant evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +intersection_of: ECO:0000214 ! biological aspect of descendant evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IBD" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ibd-inferred-biological-aspect-descendent xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:25:09Z + +[Term] +id: ECO:0000320 +name: phylogenetic determination of loss of key residues evidence used in manual assertion +def: "A type of phylogenetic determination of loss of key residues evidence that is used in a manual assertion." [ECO:MCC] +synonym: "IKR" EXACT [GOECO:IKR] +synonym: "IMR" EXACT [GOECO:IKR] +synonym: "inferred from key residues" EXACT [GOECO:IKR] +synonym: "inferred from missing residues" EXACT [GOECO:IKR] +xref: GOECO:IKR "inferred from key residues" +xref: GOECO:IMR "inferred from missing residues" +is_a: ECO:0000216 ! phylogenetic determination of loss of key residues evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +intersection_of: ECO:0000216 ! phylogenetic determination of loss of key residues evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IKR" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ikr-inferred-key-residues xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:25:48Z + +[Term] +id: ECO:0000321 +name: rapid divergence from ancestral sequence evidence used in manual assertion +def: "A type of rapid divergence from ancestral sequence evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_gene +subset: valid_with_protein +synonym: "inferred from rapid divergence" EXACT [GOECO:IRD] +synonym: "IRD" EXACT [GOECO:IRD] +xref: GOECO:IRD "inferred from rapid divergence" +is_a: ECO:0000215 ! rapid divergence from ancestral sequence evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +intersection_of: ECO:0000215 ! rapid divergence from ancestral sequence evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IRD" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ird-inferred-rapid-divergence xsd:string +created_by: mchibucos +creation_date: 2011-10-28T05:26:42Z + +[Term] +id: ECO:0000322 +name: imported manually asserted information used in automatic assertion +def: "Evidence that was initially used in manual assertion by one person or group, which is imported by a second person or group and used in automatic assertion." [ECO:MCC] +is_a: ECO:0000313 ! imported information used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2011-12-13T12:04:48Z + +[Term] +id: ECO:0000323 +name: imported automatically asserted information used in automatic assertion +def: "A type of imported information used in automatic assertion that was initially used in automatic assertion by one person or group, which is imported by a second person or group and used in automatic assertion." [ECO:MCC] +is_a: ECO:0000313 ! imported information used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2011-12-13T02:06:25Z + +[Term] +id: ECO:0000324 +name: imaging assay evidence +alt_id: ECO:0001122 +alt_id: ECO:0005024 +def: "A type of direct assay evidence in which an image is created and analyzed." [] +synonym: "radiologic test evidence" NARROW [] +xref: ECO:MCC +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000112 "In the images shown in Figure 9, the MelR focus is 15 x 33 nm across the shortest and longest axes of the oval in the complex with the shortened arm (right hand panel), compared to 9 x 12 nm in the other complex (centre panel). The simplest explanation for these observations, that is consistent with the location of sites in the DNA fragment (Figure 9), is that one type of complex contains 3 MelR molecules bound at sites 2, 1 and 1' (centre panel), whilst the other contains 4 MelR molecules bound at sites 2, 1, 1' and R (right panel)." xsd:string {xref="PMID:18346968"} +created_by: mchibucos +creation_date: 2012-02-23T12:01:43Z + +[Term] +id: ECO:0000325 +name: chromatography evidence +def: "A type of experimental evidence that is based on separation of constituent parts of a mixture (the mobile phase) as they pass differentially through a stationary phase due to differences in partition coefficient and retention on the stationary phase." [ECO:MCC] +synonym: "chromatographic evidence" EXACT [] +is_a: ECO:0000006 ! experimental evidence +created_by: mchibucos +creation_date: 2012-05-16T11:46:27Z + +[Term] +id: ECO:0000326 +name: transcript splice pattern evidence +def: "A type of sequence alignment evidence that is based on comparing the position of exon junctions, relative to a reference genome, for a transcript annotation and a supporting transcript." [ECO:MCC] +is_a: ECO:0000200 ! sequence alignment evidence +created_by: mchibucos +creation_date: 2012-06-08T11:03:59Z + +[Term] +id: ECO:0000327 +name: whole transcript splice pattern evidence +def: "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features." [ECO:MCC] +comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child. +is_a: ECO:0000326 ! transcript splice pattern evidence +created_by: mchibucos +creation_date: 2012-06-08T11:11:24Z + +[Term] +id: ECO:0000328 +name: coding sequence splice pattern evidence +def: "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript." [ECO:MCC] +comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child. +is_a: ECO:0000326 ! transcript splice pattern evidence +created_by: mchibucos +creation_date: 2012-06-08T11:21:57Z + +[Term] +id: ECO:0000329 +name: whole transcript splice pattern evidence used in manual assertion +def: "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in manual assertion." [ECO:MCC] +comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child. +is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion +is_a: ECO:0000327 ! whole transcript splice pattern evidence +intersection_of: ECO:0000327 ! whole transcript splice pattern evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISA" xsd:string +created_by: mchibucos +creation_date: 2012-06-08T11:40:03Z + +[Term] +id: ECO:0000330 +name: coding sequence splice pattern evidence used in manual assertion +def: "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in manual assertion." [ECO:MCC] +comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child. +is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion +is_a: ECO:0000328 ! coding sequence splice pattern evidence +intersection_of: ECO:0000328 ! coding sequence splice pattern evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISA" xsd:string +created_by: mchibucos +creation_date: 2012-06-08T11:43:47Z + +[Term] +id: ECO:0000331 +name: coding sequence splice pattern evidence used in automatic assertion +def: "A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in automatic assertion." [ECO:MCC] +comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child. +is_a: ECO:0000328 ! coding sequence splice pattern evidence +is_a: ECO:0007825 {is_inferred="true"} ! transcript splice pattern evidence used in automatic assertion +intersection_of: ECO:0000328 ! coding sequence splice pattern evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2012-06-08T11:48:09Z + +[Term] +id: ECO:0000332 +name: whole transcript splice pattern evidence used in automatic assertion +def: "A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in automatic assertion." [ECO:MCC] +comment: This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child. +is_a: ECO:0000327 ! whole transcript splice pattern evidence +is_a: ECO:0007825 {is_inferred="true"} ! transcript splice pattern evidence used in automatic assertion +intersection_of: ECO:0000327 ! whole transcript splice pattern evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2012-06-08T11:49:17Z + +[Term] +id: ECO:0000333 +name: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence +def: "A type of gel electrophoresis evidence where proteins are separated according to their electrophoretic mobility." [ECO:MCC, PubMed:4861258, url:http\://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html, url:http\://www.ncbi.nlm.nih.gov/Class/NAWBIS/Modules/Protein/protein18.html] +comment: Electrophoretic mobility is a function of both the length and charge of a protein. The protein sample is linearized with sodium dodecyl sulfate (SDS), an anionic detergent. Typically, polypeptides bound with SDS possess an even distribution of charge per unit mass, and subsequent fractionation is by size. +synonym: "SDS-PAGE evidence" EXACT [] +is_a: ECO:0000337 ! gel electrophoresis evidence +created_by: mchibucos +creation_date: 2012-07-31T02:56:43Z + +[Term] +id: ECO:0000334 +name: particle size and count assay evidence +def: "A type of direct assay evidence resulting from an assay where both the size of and number of particles in a sample are determined." [ECO:MCC] +comment: An example of an instrument used to analyze particle sizes and counts is the Beckman Coulter® Z Series system. +is_a: ECO:0000002 ! direct assay evidence +created_by: mchibucos +creation_date: 2012-07-31T03:19:32Z + +[Term] +id: ECO:0000335 +name: substance quantification evidence +def: "A type of direct assay evidence where the quantity of a substance used as part of an assay is measured." [ECO:MCC] +comment: Two examples of substance quantification assay are (i) measuring how much of a radiolabelled compound is taken up by a cell and (ii) measuring how much of a substrate is turned over by a given enzyme. +is_a: ECO:0000002 ! direct assay evidence +created_by: mchibucos +creation_date: 2012-07-31T03:52:46Z + +[Term] +id: ECO:0000336 +name: competitive growth assay evidence +def: "A type of cell proliferation assay evidence in which a mutated strain of a microorganism, such as a yeast or bacterium, is grown competitively with wild-type cells and the relative fitness of the strains is assessed usually by means of a fluorescent marker and flow cytometric analysis." [GOC:MAH, PMID:14718172, PMID:20537132] +synonym: "fitness profiling" EXACT [GOC:MAH, PMID:20537132] +is_a: ECO:0005007 ! cell proliferation assay evidence +created_by: mchibucos +creation_date: 2012-08-24T12:03:58Z + +[Term] +id: ECO:0000337 +name: gel electrophoresis evidence +alt_id: ECO:0005026 +def: "A type of direct assay evidence where molecules have been sorted according to their size and charge by moving through a gel in the presence of an electric field." [ECO:MCC, wikipedia:Gel_electrophoresis] +comment: Molecules typically separated include DNA, RNA, or protein. The gel is typically made of agarose, polyacrylamide, or starch. +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000112 "We analyzed the electrophoretic pattern of cytosolic proteins extracted from LM3 and LM3-2 cells, grown under standard conditions up to mid-exponential phase (Fig. 1). The presence of differentially expressed proteins in the two strains, together with results of previous studies [28,29] demonstrated the role of CcpA as global regulator in L. plantarum." xsd:string {xref="PMID:17129387"} +created_by: mchibucos +creation_date: 2012-08-24T12:11:51Z + +[Term] +id: ECO:0000338 +name: pulsed-field gel electrophoresis evidence +def: "A type of gel electrophoresis evidence in which large DNA molecules are separated on agarose gel by alternately pulsed, perpendicularly oriented electrical fields, at least one of which is inhomogeneous." [PMID:6373014] +synonym: "PFGE" EXACT [] +is_a: ECO:0000337 ! gel electrophoresis evidence +created_by: mchibucos +creation_date: 2012-08-24T12:34:01Z + +[Term] +id: ECO:0000339 +name: two-dimensional agarose gel electrophoresis evidence +def: "A type of gel electrophoresis evidence where DNA molecules are electrophoresed on a low percentage agarose gel followed by high voltage electrophoresis on a higher percentage agarose gel in the presence of ethidium bromide." [PMID:16118435, PMID:8594382] +comment: The first dimension gel is intentionally run at low voltage in low percentage agarose to separate DNA molecules in proportion to their mass. The second dimension is run at high voltage in a gel of higher agarose concentration in the presence of ethidium bromide so that the mobility of a non-linear molecule is drastically influenced by its shape. +synonym: "2-D agarose gel electrophoresis" EXACT [] +is_a: ECO:0000337 ! gel electrophoresis evidence +created_by: mchibucos +creation_date: 2012-08-24T12:42:37Z + +[Term] +id: ECO:0000340 +name: plasmid maintenance assay evidence +def: "A type of experimental evidence in which a plasmid that is capable of being replicated is introduced into cells, cells are grown without selection for several generations, and retention of the plasmid is determined." [] {comment="ECO:MCC", comment="PMID:6323245"} +comment: Loss per generation is determined by comparing colony numbers on selective and non-selective media. +synonym: "minichromosome maintenance assay evidence" EXACT [PMID:6323245] +is_a: ECO:0000006 ! experimental evidence +created_by: mchibucos +creation_date: 2012-08-24T01:27:58Z + +[Term] +id: ECO:0000341 +name: specific protein inhibition by antibody evidence +def: "A type of specific protein inhibition evidence where the molecular function of a protein is inhibited by an antibody." [ECO:MCC] +is_a: ECO:0000020 ! protein inhibition evidence +created_by: mchibucos +creation_date: 2012-08-24T01:48:24Z + +[Term] +id: ECO:0000342 +name: support of intron positions by RNA-sequencing alignment evidence +def: "A type of RNA-seq evidence where intron locations in predicted transcripts are compared to intron locations supported by RNA-seq evidence." [ECO:MCC] +comment: RNA-seq alignment intron location evidence is used by NCBI RefSeq (http://www.ncbi.nlm.nih.gov/refseq). Exon-exon boundaries (or intron locations) of a RefSeq transcript, as determined by aligning the transcript to a reference chromosome, are compared to intron locations supported by RNAseq data. +synonym: "Support of intron positions by RNA-seq alignment evidence" EXACT [] +is_a: ECO:0000295 ! RNA-sequencing evidence +created_by: mchibucos +creation_date: 2013-04-03T04:22:05Z + +[Term] +id: ECO:0000343 +name: full support of intron positions by RNA-sequencing alignment evidence +def: "A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignment from a single sample supports all of the intron positions (exon pairs) predicted for a transcript." [ECO:MCC] +comment: Full support entails that all exon pairs represented in the transcript are supported. For example, for a transcript containing five exons, if liver RNA-seq reads support all four introns but brain RNA-seq supports only three introns, then the liver data fully support the transcript exons whereas the brain data do not. +synonym: "Full support of intron positions by RNA-seq alignment evidence" EXACT [] +is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence +created_by: mchibucos +creation_date: 2013-04-03T04:41:48Z + +[Term] +id: ECO:0000344 +name: partial support of intron positions by RNA-sequencing alignment evidence +def: "A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignment to a genome supports only some of the intron positions (exon pairs) predicted for a transcript." [ECO:MCC] +comment: Partial support can occur in two ways. First, one or more exon-exon pair (intron) is not supported by any RNA-seq sample included in the analysis. Second, no single RNA-seq sample provides support for all exon-exon pairs (introns) represented in a transcript, but all represented exons are supported by at least one sample. +synonym: "Partial support of intron positions by RNA-seq alignment evidence" EXACT [] +is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence +created_by: mchibucos +creation_date: 2013-04-03T04:50:46Z + +[Term] +id: ECO:0000345 +name: single exon transcript confirmation via alignment evidence +def: "A type of sequence alignment evidence resulting from the comparison of a single exon transcript to a collection / database of known single exon genes." [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897596/] +is_a: ECO:0000200 ! sequence alignment evidence +created_by: mchibucos +creation_date: 2013-04-03T05:10:34Z + +[Term] +id: ECO:0000346 +name: support of intron positions by RNA-sequencing alignment evidence used in manual assertion +def: "A type of support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion." [ECO:MCC] +synonym: "Support of intron positions by RNA-seq alignment evidence used in manual assertion" EXACT [] +is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence +is_a: ECO:0006068 {is_inferred="true"} ! RNA-sequencing evidence used in manual assertion +intersection_of: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2013-04-04T04:37:35Z + +[Term] +id: ECO:0000347 +name: support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +def: "A type of support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." [ECO:MCC] +synonym: "Support of intron positions by RNA-seq alignment evidence used in automatic assertion" EXACT [] +is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence +is_a: ECO:0006069 {is_inferred="true"} ! RNA-sequencing evidence used in automatic assertion +intersection_of: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: mchibucos +creation_date: 2013-04-04T04:38:45Z + +[Term] +id: ECO:0000348 +name: full support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +def: "A type of full support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." [ECO:MCC] +synonym: "Full support of intron positions by RNA-seq alignment evidence used in automatic assertion" EXACT [] +is_a: ECO:0000343 ! full support of intron positions by RNA-sequencing alignment evidence +is_a: ECO:0000347 {is_inferred="true"} ! support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +intersection_of: ECO:0000343 ! full support of intron positions by RNA-sequencing alignment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: mchibucos +creation_date: 2013-04-04T04:40:19Z + +[Term] +id: ECO:0000349 +name: full support of intron positions by RNA-sequencing alignment evidence used in manual assertion +def: "A type of full support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion." [ECO:MCC] +synonym: "Full support of intron positions by RNA-seq alignment evidence used in manual assertion" EXACT [] +is_a: ECO:0000343 ! full support of intron positions by RNA-sequencing alignment evidence +is_a: ECO:0000346 {is_inferred="true"} ! support of intron positions by RNA-sequencing alignment evidence used in manual assertion +intersection_of: ECO:0000343 ! full support of intron positions by RNA-sequencing alignment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2013-04-04T04:41:34Z + +[Term] +id: ECO:0000350 +name: partial support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +def: "A type of partial support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." [ECO:MCC] +synonym: "Partial support of intron positions by RNA-seq alignment evidence used in automatic assertion" EXACT [] +is_a: ECO:0000344 ! partial support of intron positions by RNA-sequencing alignment evidence +is_a: ECO:0000347 {is_inferred="true"} ! support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +intersection_of: ECO:0000344 ! partial support of intron positions by RNA-sequencing alignment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: mchibucos +creation_date: 2013-04-04T04:42:53Z + +[Term] +id: ECO:0000351 +name: partial support of intron positions by RNA-sequencing alignment evidence used in manual assertion +def: "A type of partial support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion." [ECO:MCC] +synonym: "Partial support of intron positions by RNA-seq alignment evidence used in manual assertion" EXACT [] +is_a: ECO:0000344 ! partial support of intron positions by RNA-sequencing alignment evidence +is_a: ECO:0000346 {is_inferred="true"} ! support of intron positions by RNA-sequencing alignment evidence used in manual assertion +intersection_of: ECO:0000344 ! partial support of intron positions by RNA-sequencing alignment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: mchibucos +creation_date: 2013-04-04T04:43:56Z + +[Term] +id: ECO:0000352 +name: evidence used in manual assertion +def: "A type of evidence that is used in an manual assertion." [ECO:MCC] +is_a: ECO:0000000 {is_inferred="true"} ! evidence +intersection_of: ECO:0000000 ! evidence +intersection_of: used_in ECO:0000218 ! manual assertion +relationship: used_in ECO:0000218 {is_inferred="true"} ! manual assertion +created_by: mchibucos + +[Term] +id: ECO:0000353 +name: physical interaction evidence used in manual assertion +def: "A type of physical interaction evidence that is used in a manual assertion." [ECO:MCC] +subset: valid_with_chemical_entity +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +synonym: "inferred from physical interaction" EXACT [GOECO:IPI] +synonym: "IPI" EXACT [GOECO:IPI] +xref: GOECO:IPI "inferred from physical interaction" +is_a: ECO:0000021 ! physical interaction evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000021 ! physical interaction evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string {comment="Default"} +property_value: seeAlso http://geneontology.org/page/ipi-inferred-physical-interaction xsd:string +created_by: mchibucos +creation_date: 2013-10-31T21:01:27Z + +[Term] +id: ECO:0000354 +name: gene neighbors evidence used in manual assertion +def: "A type of gene neighbors evidence that is used in a manual assertion." [ECO:MCC] +synonym: "ICL" RELATED [] +synonym: "inferred from genome cluster" BROAD [] +xref: GO_REF:0000025 "operon structure as IGC evidence" +is_a: ECO:0000084 ! gene neighbors evidence +is_a: ECO:0000317 {is_inferred="true"} ! genomic context evidence used in manual assertion +intersection_of: ECO:0000084 ! gene neighbors evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IGC" xsd:string +created_by: mchibucos +creation_date: 2013-11-19T13:37:24Z + +[Term] +id: ECO:0000355 +name: phylogenetic distribution evidence +def: "A type of phylogenetic evidence characterized by the mapping of the character states of living organisms onto phylogenies using the method of maximum parsimony." [ECO:MCC, PMID:21238344] +comment: Example of use: "The presence of paired evaginated hemispheres and olfactory bulbs in both agnathan and gnathostome radiations suggests that such hemispheres were also present in the common ancestor." PMID: 7013637. Northcutt RG. (1981) Evolution of the telencephalon in nonmammals. Ann. Rev. Neurosci. +is_a: ECO:0000080 ! phylogenetic evidence +created_by: mchibucos +creation_date: 2014-04-22T17:02:31Z + +[Term] +id: ECO:0000358 +name: differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) +def: "A type of transcript expression evidence resulting from a microarray analysis with the use of the Gene Set Enrichment Analysis (GSEA) method or the Fisher's-Exact (FE) method to assess the differential expression." [PMID:19725948] +is_a: ECO:0000009 ! transcript expression evidence +created_by: mchibucos +creation_date: 2014-09-26T16:25:47Z + +[Term] +id: ECO:0000359 +name: differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) +def: "A type of transcript expression evidence resulting from the use of the Gene Set Enrichment Analysis (GSEA) method or Fisher's-Exact (FE) method to assess an RNA-seq dataset for differential gene expression." [PMC:2881125] +is_a: ECO:0000009 ! transcript expression evidence +created_by: mchibucos +creation_date: 2014-09-26T16:26:48Z + +[Term] +id: ECO:0000360 +name: biological target-disease association via drug evidence +def: "A type of experimental evidence resulting from the prediction of drug-target interactions by computational means." [https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-018-2199-x] +is_a: ECO:0000006 ! experimental evidence +created_by: mchibucos +creation_date: 2014-09-26T16:39:11Z + +[Term] +id: ECO:0000361 +name: inferential evidence +def: "A type of evidence where an assertion is derived from another assertion via logical inference or some non-logical but rational means." [ECO:MCC] +comment: Inference is the process of deriving logical conclusions from premises known or assumed to be true, or alternatively, arriving at conclusions via some non-logical means of observation of patterns of facts, which can result in identifying new meanings and contexts for understanding. Given the evidence provided by a set of premises, deductive reasoning involves drawing conclusions that necessarily follow, i.e. are certain, whereas inductive reasoning generates conclusions that are likely to be, but are not necessarily, true. +is_a: ECO:0000000 ! evidence +created_by: mchibucos +creation_date: 2015-07-14T11:19:37Z + +[Term] +id: ECO:0000362 +name: computational inference +def: "A type of inferential evidence based on a computationally derived unary inference or an inference chain in which at least one step is \ncomputationally derived." [ECO:MCC, GOC:MC] +synonym: "evidence based on computational logical inference" EXACT [] +is_a: ECO:0000361 ! inferential evidence +is_a: ECO:0007672 ! computational evidence +created_by: mchibucos +creation_date: 2015-07-14T11:24:27Z + +[Term] +id: ECO:0000363 +name: computational inference used in automatic assertion +def: "A type of evidence based on computational logical inference that is used in automatic assertion." [ECO:MCC] +synonym: "evidence based on computational logical inference used in automatic assertion" EXACT [] +xref: GO_REF:0000108 "Automatic assignment of GO terms using logical inference, based on on inter-ontology links" +is_a: ECO:0000362 ! computational inference +is_a: ECO:0007669 {is_inferred="true"} ! computational evidence used in automatic assertion +is_a: ECO:0007832 {is_inferred="true"} ! inferential evidence used in automatic assertion +intersection_of: ECO:0000362 ! computational inference +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2015-07-14T11:35:08Z + +[Term] +id: ECO:0000364 +name: evidence based on logical inference from manual annotation used in automatic assertion +def: "A type of evidence based on logical inference from a manually curated annotation that is used in an automatic assertion." [ECO:MCC] +is_a: ECO:0000363 ! computational inference used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2015-07-14T11:44:19Z + +[Term] +id: ECO:0000366 +name: evidence based on logical inference from automatic annotation used in automatic assertion +def: "A type of evidence based on logical inference from an automatically curated annotation that is used in an automatic assertion." [ECO:MCC] +is_a: ECO:0000363 ! computational inference used in automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: mchibucos +creation_date: 2015-07-14T11:45:25Z + +[Term] +id: ECO:0000501 +name: evidence used in automatic assertion +def: "A type of evidence that is used in an automatic assertion." [ECO:cjm] +subset: valid_with_gene +subset: valid_with_protein +subset: valid_with_protein_complex +is_a: ECO:0000000 {is_inferred="true"} ! evidence +intersection_of: ECO:0000000 ! evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +relationship: used_in ECO:0000203 {is_inferred="true"} ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +property_value: seeAlso http://geneontology.org/page/automatically-assigned-evidence-codes xsd:string +created_by: cjm + +[Term] +id: ECO:0001001 +name: 3D cell culture evidence +def: "A type of cell proliferation assay evidence resulting from the growth of cells in a micro-environment that mimics real tissue and establishes physiological cell-cell and cell-substrate interactions that regulate proliferation and differentiation." [PMID:21042962, url:http\://volttecnologia.com.br/wp-content/uploads/2016/03/Drug-discovery.pdf] +is_a: ECO:0005007 ! cell proliferation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001002 +name: [3H]arachidonic acid release assay evidence +def: "A type of cell-based assay evidence resulting from labeling cells with [3H]arachidonic acid and subsequently determining the release of the arachidonic acid by the cells." [ISBN:0323152295, PMID:20693009] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001003 +name: [3H]-thymidine incorporation assay evidence +def: "A type of DNA synthesis cell proliferation assay evidence resulting from the measurement of cell proliferation rate by determining the incorporation of [3H]-thymidine into cellular nucleic acids." [OBI:0000669, url:http\://www.scientistsolutions.com/forum/cell-culture-and-tissue-culture-proliferationapoptosis/3h-thymidine-incorporation-assay-rat-1a] +is_a: ECO:0005008 ! DNA synthesis cell proliferation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001004 +name: 51Cr release assay evidence +def: "A type of cytotoxicity assay evidence resulting from the measurement of the amount of radioactive 51Cr released in the supernatant of a sample of 51Cr pre-labeled cells via cytolysis by effector cells (cytotoxic T-cells)." [PMC:3929704, url:http\://www4.mpbio.com/ecom/docs/proddata.nsf/5f64ffd4f38c2fda8525645d00769d68/53d2a75653615bab852568cb00572ff3?OpenDocument] +is_a: ECO:0005012 ! cytotoxicity assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001005 +name: 7-aminoactinomycin staining evidence +def: "A type of apoptotic assay evidence resulting from the use of 7-aminoactinomycin D (7-AAD) to distinguish viable, apoptotic, and dead cells, using the fact that permeability of the cell membrane, and fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells." [ECO:RCT] +synonym: "7-AAD staining evidence" EXACT [] +synonym: "7-amino-actinomycin D staining evidence" EXACT [] +is_a: ECO:0005019 ! staining evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001006 +name: adhesion assay evidence +def: "A type of cell-based assay evidence resulting from the analysis of cell binding either to extracellular matrix proteins or other cells." [PMID:15576904, PMID:21909903] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001007 +name: adoptive cell transfer evidence +def: "A type of ex vivo assay evidence derived by ex vivo selection of tumor-reactive lymphocytes, and their activation and numerical expansion before re-infusion to the autologous tumor-bearing host." [ECO:SN, url:http\://jco.ascopubs.org/content/23/10/2346.full.pdf+html, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf] +synonym: "Adoptive immunotherapy" EXACT [] +synonym: "passive immunization" EXACT [] +is_a: ECO:0005502 ! ex vivo assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001008 +name: alamarBlue assay evidence +def: "A type of cell viability assay evidence resulting from the continuous measurement of fluorescence intensity from the viable cells, where a cell permeable compound, resazurin (the active ingredient of alamarBlue), is reduced to fluorescent resorufin." [PMC:3478843, url:https\://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/cell-profilteration-assay-protocols/cell-viability-with-alamarblue.html] +is_a: ECO:0005004 ! cell viability assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001009 +name: allograft transplantation phenotypic evidence +def: "A type of anatomical perturbation phenotypic evidence resulting from the transplantation of an organ or tissue from one individual of the same species with a different genotype." [ECO:SN, url:http\://www.nature.com/subjects/allograft, url:http\://www.organdonor.gov/about/terms_and_topics/] +synonym: "allograft transplantation experiment evidence" EXACT [] +synonym: "allografting" EXACT [] +synonym: "Allotransplantation" EXACT [] +synonym: "tissue grafting" NARROW [] +is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001010 +name: anion-exchange chromatography evidence +def: "A type of chromatography evidence where a positively charged ion exchange resin with an affinity for molecules having net negative surface charges is used to separate the molecules." [ECO:SN, PMID:20978968, url:http\://www.bio-rad.com/en-us/applications-technologies/anion-exchange-chromatography, url:http\://www.uta.edu/faculty/sawasthi/Enzymology-4351-5324/Class%20Syllabus%20Enzymology/Ion%20Exchange%20Chromatography-1.pdf] +is_a: ECO:0000325 ! chromatography evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001011 +name: annexin-V staining evidence +def: "A type of apoptotic assay evidence resulting from the addition and subsequent binding of Annexin V to phosphatidylserine in cells to detect if they are viable, apoptotic, or necrotic." [PMC:3169266] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001012 +name: cognitive assay phenotypic evidence +def: "A type of experimental phenotypic evidence resulting from behavioral phenotyping and analysis to assess cognitive functioning." [PMID:24462904] +synonym: "behavioral assay evidence" EXACT [] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001013 +name: blocking monoclonal antibody evidence +def: "A type of immunological assay evidence resulting from inhibitory effect of monoclonal antibody combining with an antigen over other antibodies." [PMID:11292349, url:http\://www.sciencedirect.com/science/article/pii/S0140673600034966] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001014 +name: blocking peptide evidence +def: "A type of immunological assay evidence resulting from the use of peptides to block antibodies from binding to their targets." [ECO:RCT] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001015 +name: blocking polyclonal antibody evidence +def: "A type of immunological assay evidence resulting from the inhibitory effect of polyclonal antibodies (antibodies secreted by different B cell lineages), which while not reacting with a specific antigen, serve to prevent other antibodies from doing so." [] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001016 +name: blood test evidence +def: "A type of direct assay evidence where a blood sample is extracted from an organism to analyze different blood components." [ECO:SN, url:https\://www.nhlbi.nih.gov/health/health-topics/topics/bdt] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001017 +name: Boyden chamber assay evidence +def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by measuring the number of cells that migrate from the upper compartment of a chamber (separated by a micro-porous membrane) to the lower compartment, in which chemotactic agents are present." [PMID:13872176, PMID:15576901] +is_a: ECO:0005021 ! chemotaxis assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001018 +name: bromodeoxyuridine incorporation assay evidence +alt_id: ECO:0005010 +def: "A type of nucleotide analog incorporation evidence resulting from the incorporation of bromodeoxyuridine (BrdU) as a thymidine analog into nuclear DNA, resulting in a label that can be tracked using antibody probes." [PMID:23690005] +synonym: "5-bromo-2'-deoxyuridine incorporation assay evidence" EXACT [] +synonym: "BrdU incorporation assay evidence" EXACT [] +synonym: "BrdUrd incorporation assay evidence" EXACT [] +synonym: "BUdR incorporation assay evidence" EXACT [] +synonym: "cell proliferation marker detection assay evidence" BROAD [] +is_a: ECO:0001107 ! nucleotide analog incorporation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001019 +name: caspase assay evidence +def: "A type of apoptotic assay evidence resulting from the detection of caspase activation, which results in the cleaving of intracellular substrates during apoptosis." [PMID:18314058, PMID:25086023] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001020 +name: cell counting evidence +def: "A type of cell-based assay evidence resulting from the quantification of a sample of cells." [ECO:RCT] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001021 +name: cell permeability assay evidence +def: "A type of cell-based assay evidence resulting from the quantification of cell permeability." [PMID:16962665] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001022 +name: carboxyfluorescein diacetate succinimidyl ester staining evidence +def: "A type of staining evidence resulting from monitoring division by the use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to label intracellular molecules with carboxyfluorescein; when a labeled cell divides, the number of carboxyfluorescein-tagged molecules is split." [PMC:3185625] +synonym: "CFDA-SE staining evidence" EXACT [] +synonym: "CFSE-staining evidence" EXACT [] +is_a: ECO:0005019 ! staining evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001023 +name: chemiluminescence-linked immunoassay evidence +def: "A type of protein detection assay evidence resulting from a complex (a chemiluminescent compound, protein and a steroidal hapten) utilized as a labeled antigen, which emits light when treated with hydrogen peroxide and copper acetate at a high pH." [PMID:659901] +synonym: "CLIA evidence" EXACT [] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001024 +name: chimeric protein phenotypic evidence +def: "A type of mutant phenotype evidence resulting from the fusion of two or more different genes to create one protein product." [PMID:22588898, PMID:25832756] +synonym: "chimeric protein evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001025 +name: co-electrophoresis evidence +def: "A type of gel electrophoresis evidence resulting from the electrophoresis of two substances together, allowing for the characterization of ligand/nucleic acid binding interactions." [PMID:7668395] +is_a: ECO:0000337 ! gel electrophoresis evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001026 +name: co-localization evidence +def: "A type of imaging assay evidence resulting from the limited observation of the co-occurrence of molecules in a subcellular location." [PMC:3074624] +is_a: ECO:0000324 ! imaging assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001027 +name: colony counting evidence +def: "A type of direct assay evidence resulting from the counting of microbial colonies that arise from viable cells grown on a plate or dish." [PMID:16558698, PMID:23457446] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001028 +name: co-sedimentation assay evidence +def: "A type of physical interaction evidence resulting from the separation of a mixture of molecules under the influence of a force such as artificial gravity, where molecules sedimenting together are assumed to interact." [MI:0027] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001029 +name: comet assay evidence +def: "A type of gel electrophoresis evidence resulting in the assessment of DNA breakage in a cell, based on size and shape of DNA migration." [OBI:0302736, PMID:10737956] +is_a: ECO:0000337 ! gel electrophoresis evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001030 +name: conditional knockout evidence +def: "A type of knockout evidence resulting from a temporally-restricted and/or tissue-specific targeted gene removal." [PMC:3572410] +is_a: ECO:0001091 ! knockout phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001031 +name: conditional knockin evidence +def: "A type of knockin evidence resulting from the targeted introduction of a temporally-restricted and/or tissue-specific mutation." [PMID:16832820] +is_a: ECO:0001090 ! knockin evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001032 +name: constitutively active mutant evidence +def: "A type of experimental phenotypic evidence resulting from a constitutively active mutant (CAM), resulting in mutant proteins that remain active in the absence of upstream signals." [PMID:12217490] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001033 +name: cross-linking evidence +def: "A type of protein binding evidence resulting from the identification of two interacting proteins (that exist in close proximity) by linking through covalent bonds followed by identification" [PMID:15530987, PMID:19241040] +is_a: ECO:0000024 ! protein binding evidence +property_value: IAO:0000112 "To confirm this intriguing observation, we cross-linked AdpA and DNA in the presence of increasing amounts of ATP (figure 2b(iii)). In this assay, ATP prevented the formation of AdpA - DNA complexes in a concentration-dependent manner, an outcome consistent with the results obtained by SPR analysis." xsd:string {xref="PMID:22870392"} +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001034 +name: crystallography evidence +def: "A type of structure determination evidence resulting from the use of a narrow beam of x-rays to identify molecular structures by creating a diffraction pattern." [MI:0114] +is_a: ECO:0005031 ! structure determination evidence +property_value: IAO:0000112 "The crystal structures of E. coli MarR and Enterococcus faecalis SlyA-like proteins show their cross-sections are ~70 angstroms, suggesting they would protect ~20 bp of DNA (Alekshun et al., 2001; Wu et al., 2003). This suggests the presence of two SlyA dimers at the SlyA I site and at least three dimers at the SlyA II site." xsd:string {xref="PMID:17892462"} +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001035 +name: cytochemistry evidence +def: "A type of histochemistry evidence resulting from localizing chemical components of cells and organelles on histological sections." [PMID:11597006] +is_a: ECO:0001050 ! histochemistry evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001036 +name: cytochrome C release assay evidence +def: "A type of apoptotic assay evidence resulting from the identification of an accumulation of cytochrome C in the cytoplasm after its release from the mitochondria, an early event in apoptosis." [PMID:10914021, PMID:12815469] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001037 +name: 4',6-diamidino-2-phenylindole staining evidence +def: "A type of staining evidence resulting from 4',6-diamidino-2-phenylindole (DAPI) binding to AT regions of DNA and emitting a blue fluorescence." [PMID:8580206, url:https\://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/protocols/dapi-imaging-protocol.html] +synonym: "DAPI staining evidence" EXACT [] +synonym: "diaminophenylindole staining" EXACT [] +is_a: ECO:0005019 ! staining evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001038 +name: deletion mutation phenotypic evidence +alt_id: ECO:0005510 +def: "A type of mutant phenotype evidence resulting from a mutation in which there is the removal of one or more contiguous nucleotides that may result in altered function or other measurable properties." [SO:0000159] +comment: As a child of 'experimental phenotypic evidence', this is not to be confused with the biological processes deletion mutation / deletion deficiency in which DNA is lost during DNA replication. +comment: Contrast with knockout phenotypic evidence in which any number of changes to the DNA result in elimination of the function of the gene in question. +comment: The length of the DNA deleted can range from a single nucleotide to enough material that could include multiple genes. +synonym: "deletion mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000112 "In contrast, and in full agreement with the previous results of Wade et al. (17), the longer deletion in the TB23 fragment, that removes MelR binding site 2, results in a sharp reduction in the repression of the melR promoter by MelR." xsd:string {xref="PMID:18346968"} +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001039 +name: DNA laddering assay evidence +def: "A type of apoptotic assay evidence resulting from the visualization via gel electrophoresis of the fragmented DNA (DNA ladder) which is the result of apoptotic DNA fragmentation." [PMC:4401164] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001040 +name: RNA dot blot assay evidence +def: "A type of RNA detection assay evidence based on the direct application of a RNA mixture in a circular motion on a matrix for hybridization with labeled DNA fragments." [ECO:SW, PMID:21424648] +synonym: "RNA dot blot" RELATED [] +is_a: ECO:0005518 ! RNA detection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001042 +name: dominant-negative mutant phenotypic evidence +def: "A type of mutant phenotype evidence resulting from a mutated gene producing mutant polypeptides that disrupt the activity of the wild-type genes." [PMC:2217636, PMID:8018332] +synonym: "dominant-negative mutant evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001043 +name: Edman degradation evidence used in manual assertion +def: "A type of Edman degradation evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001044 ! Edman degradation evidence +intersection_of: ECO:0001044 ! Edman degradation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:59:00Z + +[Term] +id: ECO:0001044 +name: Edman degradation evidence +def: "A type of protein detection assay evidence resulting from labeling a termonal amino acid residue and cleaving it from a peptide by sequentially removing one residue at a time from the amino end of the peptide, without disrupting the bonds." [doi:10.3891/acta.chem.scand.04-0283, OBI:0000705] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001045 +name: eTag assay evidence +def: "A type of affinity evidence resulting from the quantitative analysis of gene and protein expression monitored by proprietary eTag reporters." [PMID:15137949] +is_a: ECO:0000023 ! affinity evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001046 +name: filter binding assay evidence +def: "A type of physical interaction evidence resulting from a mixture of two molecules passed through a nitrocellulose filter, where one may be immobilized on the filter, and if the immobilized molecule is capable of binding to the other, it will be retained on the filter as well." [PMID:23028069] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001047 +name: fluorescence in situ hybridization evidence +def: "A type of nucleic acid localization evidence and in situ hybridization evidence resulting from the use of fluorescent probes to detect complementary sequences of nucleic acids." [PMC:346675, PMID:6812046] +is_a: ECO:0001534 ! nucleic acid localization evidence +is_a: ECO:0001836 ! in situ hybridization evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001048 +name: fluorescence resonance energy transfer evidence +def: "A type of dynamic fluorescence quenching evidence resulting from the measurement of the proximity of two fluorophores, where the energy from an excited molecular fluorophore to another fluorophore can only occur within ~10nm." [PMID:11516318, PMID:15696158] +synonym: "FRET" EXACT [] +is_a: ECO:0006273 ! dynamic fluorescence quenching evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001049 +name: gel-filtration evidence +def: "A type of chromatography evidence resulting from the exclusion of proteins based on molecular size by filtration through a porous matrix (swollen gel), from which larger molecules are excluded." [CHMO:0001011, doi:10.1038/nmeth0506-410] +is_a: ECO:0000325 ! chromatography evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001050 +name: histochemistry evidence +def: "A type of histology evidence resulting from the identification of chemical components in cells and tissues." [MMO:0000497] +is_a: ECO:0001051 ! histology evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001051 +name: histology evidence +def: "A type of imaging assay evidence resulting from the qualitative microscopic examination of cells or tissues." [OBI:0600020] +is_a: ECO:0000324 ! imaging assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001052 +name: high-performance liquid chromatography evidence +def: "A type of chromatography evidence resulting from the separation of molecules in a solute by forcing the solvent containing the sample through a column packed with nonporous particles at high pressure." [PMID:16376355] +synonym: "HPLC evidence" EXACT [] +is_a: ECO:0000325 ! chromatography evidence +property_value: IAO:0000112 "No OD increases with the mutant and the wild type were measured with DMSO provided as electron acceptor at 2 and 10 mM; however, HPLC analyses of cultures with 2 mM DMSO revealed that DMSO was completely consumed in wild type cultures, whereas no DMSO consumption was evident in the mutant cultures (Figure 3)." xsd:string {xref="PMID:21450087"} +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001053 +name: immunocytochemistry evidence +def: "A type of protein detection assay evidence resulting from the use of antibodies linked to coloring agents to localize structures in cell cultures to identify proteins." [MI:1200, OBI:0001986] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001054 +name: immunodepletion evidence +def: "A type of immunological assay evidence resulting from the use of antibodies to remove specific proteins from a sample." [BAO:0002505] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001055 +name: immunohistochemistry evidence +def: "A type of protein detection assay evidence resulting from the use of antibodies to detect proteins in localized cells of tissue sections." [OBI:0001986] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001056 +name: induced mutation evidence +def: "A type of genetic transformation evidence resulting from a mutation induced by a mutagenic compounds or irradiation." [OBI:0001154] +comment: This term was made obsolete for a couple of reasons: the def. doesn’t make sense as the suggested means of mutagenesis fail to introduce exogenous DNA (it is a child of genetic transformation evidence) and the def. is too much like random mutagenesis evidence. +property_value: IAO:0000234 SIB:PG xsd:string +is_obsolete: true +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001057 +name: in vitro acetylation assay evidence +def: "A type of acetylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] +is_a: ECO:0001546 ! acetylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001058 +name: in vitro cleavage assay evidence +def: "A type of cleavage assay evidence used in an in vitro experiment." [PMID:21121091] +is_a: ECO:0001547 ! cleavage assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001059 +name: in vitro deubiquitination assay evidence +def: "A type of deubiquitination assay evidence used in an in vitro experiment." [ECO:SN, PMID:19692941, SIB:PG] +is_a: ECO:0001552 ! deubiquitination assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001060 +name: in vitro deacetylation assay evidence +def: "A type of deacetylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] +is_a: ECO:0001548 ! deacetylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001061 +name: in vitro defarnesylation assay evidence +def: "A type of defarnesylation assay evidence used in an in vitro experiment." [ECO:SN, PMID:16126733, SIB:PG] +is_a: ECO:0001549 ! defarnesylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001062 +name: in vitro demethylation assay evidence +def: "A type of demethylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Demethylation] +is_a: ECO:0001550 ! demethylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001063 +name: in vitro desumoylation assay evidence +def: "A type of desumoylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] +is_a: ECO:0001551 ! desumoylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001064 +name: in vitro farnesylation assay evidence +def: "A type of farnesylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Farnesyltransferase] +is_a: ECO:0001553 ! farnesylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001065 +name: in vitro methylation assay evidence +def: "A type of methylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Methylation] +is_a: ECO:0001554 ! methylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001066 +name: in vitro palmitoylation assay evidence +def: "A type of palmitoylation assay evidence used in an in vitro experiment." [ECO:SN, PMID:17077383, SIB:PG] +is_a: ECO:0001555 ! palmitoylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001067 +name: in vitro phosphatase assay evidence +def: "A type of phosphatase assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Phosphatase] +is_a: ECO:0001556 ! phosphatase assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001068 +name: in vitro protein kinase assay evidence +def: "A type of protein kinase assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Protein_kinase, url:http\://en.wikipedia.org/wiki/Protein_phosphorylation] +is_a: ECO:0001558 ! protein kinase assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001069 +name: in vitro polyADP-ribosylation assay evidence +def: "A type of polyADP-ribosylation assay evidence used in an in vitro experiment." [ECO:SN, PMID:21870253, SIB:PG] +is_a: ECO:0001557 ! polyADP-ribosylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001070 +name: in vitro sumoylation assay evidence +def: "A type of sumoylation assay evidence used in an in vitro experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] +is_a: ECO:0001559 ! sumoylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001071 +name: in vitro transcription assay evidence +def: "A type of transcription assay evidence used in an in vitro experiment." [ECO:SN, ECO:SW, PMID:21125481, SIB:PG] +is_a: ECO:0000168 ! transcription assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001072 +name: in vitro translation assay evidence +def: "A type of translation assay evidence used in an in vitro experiment." [ECO:SN, PMID:18230759, SIB:PG] +is_a: ECO:0001561 ! translation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001073 +name: in vitro ubiquitination assay evidence +def: "A type of ubiquitination assay evidenceused in an in vitro experiment." [ECO:SN, PMID:19692941, SIB:PG] +is_a: ECO:0001562 ! ubiquitination assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001074 +name: in vivo acetylation assay evidence +def: "A type of acetylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] +is_a: ECO:0001546 ! acetylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001075 +name: in vivo cleavage assay evidence +def: "A type of cleavage assay evidence used in an in vivo experiment." [ECO:SN, PMID:22154596] +is_a: ECO:0001547 ! cleavage assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001076 +name: in vivo deacetylation assay evidence +def: "A type of deacetylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] +is_a: ECO:0001548 ! deacetylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001077 +name: in vivo defarnesylation assay evidence +def: "A type of defarnesylation assay evidence used in an in vivo experiment." [ECO:SN, PMID:15556768, PMID:16507103, SIB:PG] +is_a: ECO:0001549 ! defarnesylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001078 +name: in vivo demethylation assay evidence +def: "A type of demethylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Demethylation] +is_a: ECO:0001550 ! demethylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001079 +name: in vivo deubiquitination assay evidence +def: "A type of deubiquitination assay evidence used in an in vivo experiment." [ECO:SN, PMID:19692941, SIB:PG] +is_a: ECO:0001552 ! deubiquitination assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001080 +name: in vivo desumoylation assay evidence +def: "A type of desumoylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] +is_a: ECO:0001551 ! desumoylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001081 +name: in vivo farnesylation assay evidence +def: "A type of farnesylation assay evidence used in an in vivo experiment." [ECO:SN, PMID:9030603, SIB:PG] +is_a: ECO:0001553 ! farnesylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001082 +name: in vivo methylation assay evidence +def: "A type of methylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Methylation] +is_a: ECO:0001554 ! methylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001083 +name: in vivo palmitoylation assay evidence +def: "A type of palmitoylation assay evidence used in an in vivo experiment." [ECO:SN, PMID:10329400, SIB:PG] +is_a: ECO:0001555 ! palmitoylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001084 +name: in vivo phosphatase assay evidence +def: "A type of phosphatase assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Phosphatase] +is_a: ECO:0001556 ! phosphatase assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001085 +name: in vivo protein kinase assay evidence +def: "A type of protein kinase assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://en.wikipedia.org/wiki/Protein_kinase, url:http\://en.wikipedia.org/wiki/Protein_phosphorylation] +is_a: ECO:0001558 ! protein kinase assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001086 +name: in vivo sumoylation assay evidence +def: "A type of sumoylation assay evidence used in an in vivo experiment." [ECO:SN, SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] +is_a: ECO:0001559 ! sumoylation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001087 +name: in vivo transcription assay evidence +def: "A type of transcription assay evidence used in an in vivo experiment." [ECO:SN, PMID:12181418, SIB:PG] +is_a: ECO:0000168 ! transcription assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001088 +name: in vivo translation assay evidence +def: "A type of translation assay evidence used in an in vivo experiment." [ECO:SN, PMID:24901308, SIB:PG] +is_a: ECO:0001561 ! translation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001089 +name: in vivo ubiquitination assay evidence +def: "A type of ubiquitination assay evidence used in an in vivo experiment." [ECO:SN, PMID:19692941, SIB:PG] +is_a: ECO:0001562 ! ubiquitination assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001090 +name: knockin evidence +def: "A type of genetic transformation evidence resulting from the targetted replacement of a wild-type DNA sequence with a different sequence." [PMID:18077807, PMID:21800101] +is_a: ECO:0005027 ! genetic transformation evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001091 +name: knockout phenotypic evidence +def: "A type of genetic transformation evidence resulting from a change in the DNA that results in the elimination of the function of the gene, allowing for functional analysis." [PMC:2782548] +comment: A change in the DNA may result from any number of processes such (deletion, insertion, frame shift, etc.) Additionally, the gene may be made inoperative via homologous recombination, site-specific nucleases, zinc finger nucleases, transcription activator-like effector nucleases (TALENs), or CRISPR (Clustered regularly interspaced short palindromic repeats). +comment: Contrast with deletion mutation phenotypic evidence in which DNA is deleted which may result in altering the function of the resulting protein(s). +synonym: "gene deletion evidence" NARROW [] +synonym: "gene knockout evidence" NARROW [] +synonym: "knockout evidence" EXACT [] +is_a: ECO:0005027 ! genetic transformation evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001092 +name: lipid binding assay evidence +def: "A type of physical interaction evidence resulting from the qualitative and quantitative analysis of the affinity with which a protein binds to a lipid." [PMID:22848065, PMID:23681540] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001093 +name: luminescence-based mammalian interactome mapping assay evidence +def: "A type of physical interaction evidence resulting from the analysis of protein-protein interactions by use of a luciferase enzyme fused to a particular protein (or proteins), which are coexpressed with epitope-tagged partners in mammalian cells." [MI:0729, PMID:15761153] +synonym: "LUMIER assay" EXACT [] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001094 +name: macroscopy evidence +def: "A type of experimental evidence that is visible to the naked eye cf. microscopy evidence which requires the aid of a microscope." [] +is_a: ECO:0000006 ! experimental evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001095 +name: mammalian 2-hybrid assay evidence +def: "A type of bait-prey hybrid interaction evidence resulting from the analysis of proteins of interest attached to two portions of the transcriptional activator, and in interaction bring the two portions together to increase expression of the reporter gene." [ECO:RCT, PMID:9043710] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001096 +name: mass spectrometry evidence +def: "A type of spectrometry evidence resulting from identifying the amount and type of material entities present in a sample by fragmenting it and measuring the mass-to-charge ratio of the resulting particles." [OBI:0000470] +is_a: ECO:0005504 ! spectrometry evidence +property_value: IAO:0000112 "The MS analyses showed that two VapB10 peaks (836.437, 1584.789 m/z) and three VapC10-His6 peaks (1049.526, 1299.733 and 1455.853 m/z) were detected in MS-DIGEST program, indicating that both VapB10 and VapC10-His6 had the expected peptide masses (Figure S3). These results suggest that VapB10 binds to VapC10-His6 forming the TA complex VapBC10 in vivo, which may cause the counteraction of the VapC10-induced growth arrest (Figure 2)." xsd:string {xref="PMID:24260461"} +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001097 +name: medical imaging evidence +def: "A type of imaging assay evidence resulting from the use of technology to visualize and provide information about the body to diagnose, treat, or monitor medical conditions." [url:http\://www.fda.gov/Radiation-EmittingProducts/RadiationEmittingProductsandProcedures/MedicalImaging/ucm2005914.htm] +is_a: ECO:0000324 ! imaging assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001098 +name: microscopy evidence +def: "A type of direct assay evidence resulting from the use of a microscope." [ECO:MCC] +comment: Microscopy may include any of the following: optical, electron, scanning probe, or X-ray. +is_a: ECO:0000324 ! imaging assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001099 +name: motility wound healing assay evidence +alt_id: ECO:0001135 +def: "A type of cell-based assay evidence to measure cell motility in which a \"wound\" is created in a cell monolayer and the migration of the cells is captured by imaging at regular intervals during cell migration to close the wound." [] +synonym: "wound healing assay evidence" BROAD [] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001100 +name: MTS assay evidence +def: "A type of apoptotic assay evidence resulting from the reduction of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) in combination with phenazine methyl sulfate (PMS) as an intermediate electron acceptor reagent to produce a soluble formazan dye in viable cells." [NBK:144065, PMID:1867954] +synonym: "3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium" NARROW [] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001101 +name: MTT assay evidence +def: "A type of apoptotic assay evidence resulting from the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to its insoluble purple formazan dye in viable cells and measures cell viability / metabolic activity." [NBK:144065, PMID:6606682] +synonym: "3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide" NARROW [] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001102 +name: multiplex bead-based immunoassay evidence +def: "A type of protein detection assay evidence resulting from determination of analyte concentration by fluorescent bead sets attached to an antibody (to a specific analyte) with a fluorescent reporter dye label attached to a second antibody (to a specific analyte)." [PMC:1534009] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001103 +name: natural variation mutant evidence +def: "A type of experimental phenotypic evidence resulting from the observation of the impact of a natural mutation on the expressed traits." [ECO:RCT] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001104 +name: nuclear fragmentation evidence +def: "A type of apoptotic assay evidence resulting from the nuclei fragmenting into smaller pieces during programmed cell death." [PMID:16738861] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001105 +name: nuclear magnetic resonance evidence +def: "A type of magnetic resonance evidence in which atomic nuclei in a strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting electromagnetic signal is captured." [url:https\://en.wikipedia.org/wiki/Nuclear_magnetic_resonance] +is_a: ECO:0006252 ! magnetic resonance evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001106 +name: nuclease protection assay evidence +def: "A type of RNA detection assay evidence resulting from the detection and quantitation of specific RNAs (from a sample of total cellular RNA) that are bound by antisense RNA or DNA probes and therefore protected from nucleases." [PMID:17486122, PMID:18428580] +is_a: ECO:0005518 ! RNA detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001107 +name: nucleotide analog incorporation assay evidence +def: "A type of DNA synthesis cell proliferation assay evidence resulting from the analysis and measurement of the incorporation of fluorescently labeled nucleotide analog(s) into the DNA." [PMC:3149870] +is_a: ECO:0005008 ! DNA synthesis cell proliferation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001108 +name: phage display evidence +def: "A type of protein binding evidence resulting from the panning of a phage library where the individual phage displayed a different peptide or protein by fusion to coat proteins on the capsid." [MI:0084, PMID:11680867] +is_a: ECO:0000024 ! protein binding evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001109 +name: phosphoamino acid analysis evidence +def: "A type of direct assay evidence resulting from the identification of the phosphorylated residue in a protein by amino acid analysis." [PMID:11680867, PMID:18429115] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001110 +name: peptide affinity enrichment evidence +def: "A type of affinity evidence resulting from the appending of affinity tags on proteins so that they may be purified from their biological source using an affinity technique." [] +synonym: "phosphopeptide affinity enrichment evidence" NARROW [] +is_a: ECO:0000023 ! affinity evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001111 +name: physical examination evidence +def: "A type of direct assay evidence resulting from the physical examination and measurement of the feature of a subject or sample." [ECO:RCT] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001112 +name: peptide array evidence +def: "A type of protein binding evidence resulting from protein-bound peptides detected from a collection of peptides arranged as an array and incubated with the partner protein in order to map protein-protein interaction sites." [PMID:21243154] +synonym: "phosphopeptide array evidence" NARROW [] +is_a: ECO:0000024 ! protein binding evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001113 +name: point mutation phenotypic evidence +def: "A type of mutant phenotype evidence resulting from the change in a single nucleotide." [SO:1000008] +synonym: "point mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001114 +name: propidium iodide staining evidence +def: "A type of staining evidence resulting from the binding and labeling of DNA (during apoptosis, when there is a loss of nuclear DNA content) with propidium iodide (PI) to identify the cells from which it originated." [PMID:17406435] +is_a: ECO:0005019 ! staining evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001115 +name: fluorescence evidence +def: "A type of direct assay evidence resulting from the analysis of a molecule by its intrinsic fluorescence, or by attaching it with a fluorophore." [url:http\://www.nature.com/subjects/biological-fluorescence] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001116 +name: protein dot blot assay evidence +def: "A type of protein detection assay evidence resulting from protein samples immobilized on a membrane, then subsequently incubated and imaged." [doi:10.1007/978-94-009-0951-9_24] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001117 +name: protein microarray evidence +def: "A type of protein detection assay evidence resulting from proteins immobilized to prepare protein chips, or microwells, that can be utilized to determine presence of proteins, monitor differential expression profiles, and/or study protein interactions." [PMC:1828913] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001118 +name: protein sequencing assay evidence +def: "A type of sequencing assay evidence resulting from determining the sequence of amino acids in a protein." [ERO:0001287] +is_a: ECO:0000220 ! sequencing assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001119 +name: quantitative mass spectrometry evidence +def: "A type of mass spectrometry evidence resulting from quantitative analysis of peptides, proteins, and proteomes." [PMID:22665296] +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001120 +name: radioisotope assay evidence +def: "A type of direct assay evidence resulting from the assessment of biological reactions by use of a radioactive isotope to label the reactant." [ISBN:978-3-642-50036-7] +synonym: "radioassay" EXACT [] +synonym: "radioisotopic assay" EXACT [] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001121 +name: radioimmunoassay evidence +def: "A type of protein detection assay evidence resulting from the use of a radioligand to measure the binding of a substance to a specific antibody or other receptor system." [MeSH:D011863] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001123 +name: resonant mirror biosensor evidence +def: "A type of direct assay evidence from the analysis of the real-time interaction between an analyte in solution and its interactant linked to the surface of the resonant mirror." [PMID:19151938] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001124 +name: restriction fragment detection evidence +def: "A type of DNA detection assay evidence resulting from homologous DNA fragments digested by restriction enzymes, and the resulting restriction fragments are sorted by length to illustrate differences." [doi:10.1007/978-94-009-0951-9_24, url:http\://www.ncbi.nlm.nih.gov/probe/docs/techrflp/] +is_a: ECO:0005519 ! DNA detection assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001126 +name: spectrophotometry evidence +def: "A type of spectrometry evidence resulting from the evaluation of a molecule in a fluid or solid by its ability to alter the transmission of light at specific wavelengths." [MMO:0000173] +is_a: ECO:0005504 ! spectrometry evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001127 +name: surface plasmon resonance evidence +def: "A type of physical interaction evidence based on real-time, rapid plasmon generation on the interface between a planar surface and vacuum that measures changes in refractive index close to the sensor surface when an analyte and its immobilized ligand bind to observe and characterize molecular interaction." [ECO:SW, PMID:11578932, PMID:19151937, PMID:8574707] +comment: Plasmons are generated by an incident light beam. +synonym: "SPR evidence" EXACT [PMID:11578932] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000112 "Electrophoretic mobility shift assay (EMSA) and surface plasmon resonance (SPR) demonstrated that AdpAHis6 specifically bound the oriC fragment (283-bp) containing the in silico-predicted A-boxes in a concentration-dependent manner (figures 1b and 2a), but not the remaining part of oriC (data not shown)." xsd:string {xref="PMID:22870392"} +property_value: IAO:0000112 "Further confirmation of the specific interaction was obtained by conducting the competing surface plasmon resonance (SPR) assay with the unlabeled DNA fragments. As shown in Additional file 3, a significantly lower response was observed when either the unlabeled S2 or S5 was added together with MtrA, which indicated that they could compete the binding of MtrA with the promoter DNA on the chip." xsd:string {xref="PMID:20843371"} +property_value: IAO:0000112 "Our SPR analysis revealed that also housekeeping genes required for ribosome function (rplR) and beta subunit RNA polymerase (rpoB) belong to the LexA regulon, a feature of the SOS network not yet observed in bacteria." xsd:string {xref="PMID:24713082"} +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001128 +name: syngeneic transplantation experiment evidence +def: "A type of experimental phenotypic evidence resulting from a transplantation in which the transplanted material (stem cells) is from an individual's identical twin." [PMID:18469352] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001129 +name: TACE activity assay evidence +def: "A type of enzymatic activity assay evidence resulting from the analysis of tumor necrosis factor (TNF)-a converting enzyme (TACE), which releases a soluble TNF-a from the membrane-bound precursor protein." [PMC:1808921] +synonym: "Tumor necrosis factor - alpha converting enzyme activity assay" EXACT [] +is_a: ECO:0000005 ! enzymatic activity assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001130 +name: tissue microarray evidence +def: "A type of cytochemistry evidence resulting from the analysis of section(s) of a paraffin block containing embedded tissues cores arranged in an array pattern." [PMID:11770905] +is_a: ECO:0001035 ! cytochemistry evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001131 +name: transgenic organism evidence +def: "A type of genetic transformation evidence resulting from an organism that has had its expressed phenotype altered by modification." [OBI:1000048] +is_a: ECO:0005027 ! genetic transformation evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001132 +name: tryptic phosphopeptide mapping assay evidence +def: "A type of mass spectrometry evidence resulting from the analysis of prepared phosphopeptides separated in two dimensions on a TLC plate." [PMID:18429120] +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001133 +name: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence +def: "A type of apoptotic assay evidence resulting from the visualization of DNA fragmentation by detection of exposed 3'-OH ends, localized by terminal deoxynucleotidyl transferase (TdT) which then catalyzes the addition of labeled dUTPs." [PMID:22566045] +synonym: "TdT-mediated dUTP-biotin nick end labeling assay" EXACT [] +synonym: "terminal deoxynucleotidyl transferase-dUTP nick end labeling assay evidence" EXACT [] +synonym: "TUNEL assay evidence" EXACT [] +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001134 +name: urine test evidence +def: "A type of direct assay evidence resulting from the analysis of a urine sample." [ECO:RCT] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001136 +name: WST-1 assay evidence +alt_id: ECO:0005009 +def: "A type of cell proliferation assay evidence resulting from the assessment of metabolic activity from a water-soluble tetrazolium salt WST-1 being reduced outside the cell (by reacting with mitochondrial succinate-tetrazolium reductase) to form a formazan dye." [PMID:18417231] +synonym: "metabolic cell proliferation assay evidence" BROAD [] +is_a: ECO:0005007 ! cell proliferation assay evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001137 +name: xenotransplantation phenotypic evidence +def: "A type of anatomical perturbation phenotypic evidence resulting from the transplantation, implantation, or infusion of live cells, tissues, or organs between individuals of different species." [ECO:SN, url:http\://ilarjournal.oxfordjournals.org/content/37/1/16.full.pdf+html, url:http\://www.fda.gov/BiologicsBloodVaccines/Xenotransplantation/] +synonym: "tissue grafting" NARROW [] +synonym: "Xenograft transplantation" EXACT [] +synonym: "Xenografting" EXACT [] +synonym: "Xenotransplantation" EXACT [] +synonym: "xenotransplantation experiment evidence" EXACT [] +is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-16T11:30:54Z + +[Term] +id: ECO:0001138 +name: 3D cell culture evidence used in manual assertion +def: "A type of 3D cell culture evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001001 ! 3D cell culture evidence +intersection_of: ECO:0001001 ! 3D cell culture evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:18:35Z + +[Term] +id: ECO:0001139 +name: 51Cr release assay evidence used in manual assertion +def: "A type of 51Cr release assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001004 ! 51Cr release assay evidence +intersection_of: ECO:0001004 ! 51Cr release assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:20:55Z + +[Term] +id: ECO:0001140 +name: 7-aminoactinomycin staining evidence used in manual assertion +def: "A type of 7-aminoactinomycin staining evidence that is used in a manual assertion." [ECO:MCC] +synonym: "7-AAD staining evidence" BROAD [] +synonym: "7-amino-actinomycin D staining evidence" BROAD [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001005 ! 7-aminoactinomycin staining evidence +intersection_of: ECO:0001005 ! 7-aminoactinomycin staining evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:22:01Z + +[Term] +id: ECO:0001141 +name: [3H]-thymidine incorporation assay evidence used in manual assertion +def: "A type of [3H]-thymidine incorporation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001003 ! [3H]-thymidine incorporation assay evidence +intersection_of: ECO:0001003 ! [3H]-thymidine incorporation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:31:52Z + +[Term] +id: ECO:0001142 +name: [3H]arachidonic acid release assay evidence used in manual assertion +def: "A type of [3H]arachidonic acid release assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001002 ! [3H]arachidonic acid release assay evidence +intersection_of: ECO:0001002 ! [3H]arachidonic acid release assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:32:44Z + +[Term] +id: ECO:0001143 +name: adhesion assay evidence used in manual assertion +def: "A type of adhesion assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001006 ! adhesion assay evidence +intersection_of: ECO:0001006 ! adhesion assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:33:38Z + +[Term] +id: ECO:0001144 +name: adoptive cell transfer evidence used in manual assertion +def: "A type of adoptive cell transfer evidence that is used in a manual assertion." [ECO:MCC] +synonym: "Adoptive immunotherapy" BROAD [] +synonym: "passive immunization" BROAD [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001007 ! adoptive cell transfer evidence +intersection_of: ECO:0001007 ! adoptive cell transfer evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:36:19Z + +[Term] +id: ECO:0001145 +name: alamarBlue assay evidence used in manual assertion +def: "A type of alamarBlue assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001008 ! alamarBlue assay evidence +intersection_of: ECO:0001008 ! alamarBlue assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:37:21Z + +[Term] +id: ECO:0001146 +name: allograft transplantation phenotypic evidence used in manual assertion +def: "A type of allograft transplantation phenotypic evidence that is used in a manual assertion." [ECO:MCC] +synonym: "allograft transplantation evidence used in manual assertion" EXACT [] +synonym: "allografting" BROAD [] +synonym: "Allotransplantation" BROAD [] +synonym: "tissue grafting" RELATED [] +is_a: ECO:0001009 ! allograft transplantation phenotypic evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0001009 ! allograft transplantation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:38:22Z + +[Term] +id: ECO:0001147 +name: anion-exchange chromatography evidence used in manual assertion +def: "A type of anion-exchange chromatography evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001010 ! anion-exchange chromatography evidence +is_a: ECO:0007680 {is_inferred="true"} ! chromatography evidence used in manual assertion +intersection_of: ECO:0001010 ! anion-exchange chromatography evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:44:30Z + +[Term] +id: ECO:0001148 +name: annexin-V staining evidence used in manual assertion +def: "A type of annexin-V staining evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001011 ! annexin-V staining evidence +intersection_of: ECO:0001011 ! annexin-V staining evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:52:01Z + +[Term] +id: ECO:0001149 +name: cognitive assay phenotypic evidence used in manual assertion +def: "A type of cognitive assay phenotypic evidence used that is used in a manual assertion." [ECO:MCC] +synonym: "behavioral assay evidence used in manual assertion" EXACT [] +is_a: ECO:0001012 ! cognitive assay phenotypic evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0001012 ! cognitive assay phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:53:38Z + +[Term] +id: ECO:0001150 +name: blocking monoclonal antibody evidence used in manual assertion +def: "A type of blocking monoclonal antibody evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001013 ! blocking monoclonal antibody evidence +is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion +intersection_of: ECO:0001013 ! blocking monoclonal antibody evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:54:44Z + +[Term] +id: ECO:0001151 +name: blocking peptide evidence used in manual assertion +def: "A type of blocking peptide evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001014 ! blocking peptide evidence +is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion +intersection_of: ECO:0001014 ! blocking peptide evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T15:55:33Z + +[Term] +id: ECO:0001152 +name: blocking polyclonal antibody evidence used in manual assertion +def: "A type of blocking polyclonal antibody evidence tthat is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001015 ! blocking polyclonal antibody evidence +is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion +intersection_of: ECO:0001015 ! blocking polyclonal antibody evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:01:39Z + +[Term] +id: ECO:0001153 +name: blood test evidence used in manual assertion +def: "A type of blood test evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001016 ! blood test evidence +intersection_of: ECO:0001016 ! blood test evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:02:47Z + +[Term] +id: ECO:0001154 +name: Boyden chamber assay evidence used in manual assertion +def: "A type of Boyden chamber assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001017 ! Boyden chamber assay evidence +intersection_of: ECO:0001017 ! Boyden chamber assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:03:53Z + +[Term] +id: ECO:0001155 +name: bromodeoxyuridine incorporation assay evidence used in manual assertion +def: "A type of bromodeoxyuridine incorporation assay evidence used in manual assertion." [ECO:MCC] +synonym: "5-bromo-2'-deoxyuridine incorporation assay evidence" BROAD [] +synonym: "BrdU incorporation assay evidence" BROAD [] +synonym: "BrdUrd incorporation assay evidence" BROAD [] +synonym: "BUdR incorporation assay evidence" BROAD [] +is_a: ECO:0001018 ! bromodeoxyuridine incorporation assay evidence +is_a: ECO:0001241 {is_inferred="true"} ! nucleotide analog incorporation assay evidence used in manual assertion +intersection_of: ECO:0001018 ! bromodeoxyuridine incorporation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:04:49Z + +[Term] +id: ECO:0001156 +name: caspase assay evidence used in manual assertion +def: "A type of caspase assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001019 ! caspase assay evidence +intersection_of: ECO:0001019 ! caspase assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:06:06Z + +[Term] +id: ECO:0001157 +name: cell counting evidence used in manual assertion +def: "A type of cell counting evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001020 ! cell counting evidence +intersection_of: ECO:0001020 ! cell counting evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:07:13Z + +[Term] +id: ECO:0001158 +name: cell permeability assay evidence used in manual assertion +def: "A type of cell permeability assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001021 ! cell permeability assay evidence +intersection_of: ECO:0001021 ! cell permeability assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:07:58Z + +[Term] +id: ECO:0001159 +name: carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion +def: "A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in a manual assertion." [ECO:MCC] +synonym: "CFDA-SE staining evidence" BROAD [] +synonym: "CFSE-staining evidence" BROAD [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001022 ! carboxyfluorescein diacetate succinimidyl ester staining evidence +intersection_of: ECO:0001022 ! carboxyfluorescein diacetate succinimidyl ester staining evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:08:54Z + +[Term] +id: ECO:0001160 +name: chemiluminescence-linked immunoassay evidence used in manual assertion +def: "A type of chemiluminescence-linked immunoassay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "CLIA evidence" BROAD [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001023 ! chemiluminescence-linked immunoassay evidence +intersection_of: ECO:0001023 ! chemiluminescence-linked immunoassay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:09:55Z + +[Term] +id: ECO:0001161 +name: chimeric protein phenotypic evidence used in manual assertion +def: "A type of chimeric protein phenotypic evidence that is used in a manual assertion." [ECO:MCC] +synonym: "chimeric protein evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0001024 ! chimeric protein phenotypic evidence +intersection_of: ECO:0001024 ! chimeric protein phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:10:40Z + +[Term] +id: ECO:0001162 +name: co-electrophoresis evidence used in manual assertion +def: "A type of co-electrophoresis evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001025 ! co-electrophoresis evidence +intersection_of: ECO:0001025 ! co-electrophoresis evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:11:22Z + +[Term] +id: ECO:0001163 +name: co-localization evidence used in manual assertion +def: "A type of co-localization evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001026 ! co-localization evidence +is_a: ECO:0001256 {is_inferred="true"} ! imaging assay evidence used in manual assertion +intersection_of: ECO:0001026 ! co-localization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:12:04Z + +[Term] +id: ECO:0001164 +name: co-sedimentation assay evidence used in manual assertion +def: "A type of co-sedimentation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +is_a: ECO:0001028 ! co-sedimentation assay evidence +intersection_of: ECO:0001028 ! co-sedimentation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:12:55Z + +[Term] +id: ECO:0001165 +name: colony counting evidence used in manual assertion +def: "A type of colony counting evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001027 ! colony counting evidence +intersection_of: ECO:0001027 ! colony counting evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:14:57Z + +[Term] +id: ECO:0001166 +name: comet assay evidence used in manual assertion +def: "A type of comet assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001029 ! comet assay evidence +intersection_of: ECO:0001029 ! comet assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:16:01Z + +[Term] +id: ECO:0001167 +name: conditional knockin evidence used in manual assertion +def: "A type of conditional knockin evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001031 ! conditional knockin evidence +is_a: ECO:0001224 {is_inferred="true"} ! knockin evidence used in manual assertion +intersection_of: ECO:0001031 ! conditional knockin evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:17:22Z + +[Term] +id: ECO:0001168 +name: conditional knockout evidence used in manual assertion +def: "A type of conditional knockout evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001030 ! conditional knockout evidence +is_a: ECO:0001225 {is_inferred="true"} ! knockout evidence used in manual assertion +intersection_of: ECO:0001030 ! conditional knockout evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:17:58Z + +[Term] +id: ECO:0001169 +name: constitutively active mutant evidence used in manual assertion +def: "A type of constitutively active mutant evidence that is used a in manual assertion." [ECO:MCC] +is_a: ECO:0001032 ! constitutively active mutant evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0001032 ! constitutively active mutant evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:20:23Z + +[Term] +id: ECO:0001170 +name: cross-linking evidence used in manual assertion +def: "A type of cross-linking evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001033 ! cross-linking evidence +is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion +intersection_of: ECO:0001033 ! cross-linking evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:21:29Z + +[Term] +id: ECO:0001171 +name: crystallography evidence used in manual assertion +def: "A type of crystallography evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001034 ! crystallography evidence +intersection_of: ECO:0001034 ! crystallography evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:22:20Z + +[Term] +id: ECO:0001172 +name: cytochemistry evidence used in manual assertion +def: "A type of cytochemistry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001035 ! cytochemistry evidence +is_a: ECO:0001185 {is_inferred="true"} ! histochemistry evidence used in manual assertion +intersection_of: ECO:0001035 ! cytochemistry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:23:10Z + +[Term] +id: ECO:0001173 +name: cytochrome C release assay evidence used in manual assertion +def: "A type of cytochrome C release assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001036 ! cytochrome C release assay evidence +intersection_of: ECO:0001036 ! cytochrome C release assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:23:53Z + +[Term] +id: ECO:0001174 +name: 4',6-diamidino-2-phenylindole staining evidence used in manual assertion +def: "A type of 4',6-diamidino-2-phenylindole staining evidence that is used in a manual assertion." [ECO:MCC] +synonym: "DAPI staining evidence" BROAD [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001037 ! 4',6-diamidino-2-phenylindole staining evidence +intersection_of: ECO:0001037 ! 4',6-diamidino-2-phenylindole staining evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:25:03Z + +[Term] +id: ECO:0001175 +name: deletion mutation phenotypic evidence used in manual assertion +def: "A type of deletion mutation phenotypic evidence that is used in a manual assertion." [ECO:MCC] +synonym: "deletion mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0001038 ! deletion mutation phenotypic evidence +intersection_of: ECO:0001038 ! deletion mutation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:25:54Z + +[Term] +id: ECO:0001176 +name: DNA laddering assay evidence used in manual assertion +def: "A type of DNA laddering assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001039 ! DNA laddering assay evidence +intersection_of: ECO:0001039 ! DNA laddering assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:26:46Z + +[Term] +id: ECO:0001177 +name: RNA dot blot assay evidence used in manual assertion +def: "A type of RNA dot blot assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "RNA dot blot evidence" BROAD [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001040 ! RNA dot blot assay evidence +intersection_of: ECO:0001040 ! RNA dot blot assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:45:52Z + +[Term] +id: ECO:0001179 +name: dominant-negative mutant phenotypic evidence used in manual assertion +def: "A type of dominant-negative mutant phenotypic evidence that is used in a manual assertion." [ECO:MCC] +synonym: "dominant-negative mutant evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0001042 ! dominant-negative mutant phenotypic evidence +intersection_of: ECO:0001042 ! dominant-negative mutant phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:47:44Z + +[Term] +id: ECO:0001180 +name: eTag assay evidence used in manual assertion +def: "A type of eTag assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001045 ! eTag assay evidence +is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion +intersection_of: ECO:0001045 ! eTag assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T16:59:39Z + +[Term] +id: ECO:0001181 +name: filter binding assay evidence used in manual assertion +def: "A type of filter binding assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +is_a: ECO:0001046 ! filter binding assay evidence +intersection_of: ECO:0001046 ! filter binding assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:03:28Z + +[Term] +id: ECO:0001182 +name: fluorescence in situ hybridization evidence used in manual assertion +def: "A type of fluorescence in situ hybridization evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001047 ! fluorescence in situ hybridization evidence +is_a: ECO:0001838 {is_inferred="true"} ! in situ hybridization evidence used in manual assertion +intersection_of: ECO:0001047 ! fluorescence in situ hybridization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:04:20Z + +[Term] +id: ECO:0001183 +name: fluorescence resonance energy transfer evidence used in manual assertion +def: "A type of fluorescence resonance energy transfer evidence that is used in a manual assertion." [ECO:MCC] +synonym: "FRET evidence" BROAD [] +is_a: ECO:0001048 ! fluorescence resonance energy transfer evidence +is_a: ECO:0006327 {is_inferred="true"} ! dynamic fluorescence quenching evidence used in manual assertion +intersection_of: ECO:0001048 ! fluorescence resonance energy transfer evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:05:22Z + +[Term] +id: ECO:0001184 +name: gel-filtration evidence used in manual assertion +def: "A type of gel-filtration evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001049 ! gel-filtration evidence +is_a: ECO:0007680 {is_inferred="true"} ! chromatography evidence used in manual assertion +intersection_of: ECO:0001049 ! gel-filtration evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:06:14Z + +[Term] +id: ECO:0001185 +name: histochemistry evidence used in manual assertion +def: "A type of histochemistry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001050 ! histochemistry evidence +is_a: ECO:0001187 {is_inferred="true"} ! histology evidence used in manual assertion +intersection_of: ECO:0001050 ! histochemistry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:07:19Z + +[Term] +id: ECO:0001186 +name: immunocytochemistry evidence used in manual assertion +def: "A type of immunocytochemistry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001053 ! immunocytochemistry evidence +intersection_of: ECO:0001053 ! immunocytochemistry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:12:39Z + +[Term] +id: ECO:0001187 +name: histology evidence used in manual assertion +def: "A type of histology evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001051 ! histology evidence +is_a: ECO:0001256 {is_inferred="true"} ! imaging assay evidence used in manual assertion +intersection_of: ECO:0001051 ! histology evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:09:50Z + +[Term] +id: ECO:0001188 +name: immunodepletion evidence used in manual assertion +def: "A type of immunodepletion evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001054 ! immunodepletion evidence +is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion +intersection_of: ECO:0001054 ! immunodepletion evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:16:19Z + +[Term] +id: ECO:0001189 +name: immunohistochemistry evidence used in manual assertion +def: "A type of immunohistochemistry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001055 ! immunohistochemistry evidence +intersection_of: ECO:0001055 ! immunohistochemistry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:17:22Z + +[Term] +id: ECO:0001190 +name: in vitro acetylation assay evidence used in manual assertion +def: "A type of in vitro acetylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001057 ! in vitro acetylation assay evidence +intersection_of: ECO:0001057 ! in vitro acetylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:19:24Z + +[Term] +id: ECO:0001191 +name: in vitro cleavage assay evidence used in manual assertion +def: "A type of in vitro cleavage assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001058 ! in vitro cleavage assay evidence +intersection_of: ECO:0001058 ! in vitro cleavage assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:21:22Z + +[Term] +id: ECO:0001192 +name: in vitro deacetylation assay evidence used in manual assertion +def: "A type of in vitro deacetylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001060 ! in vitro deacetylation assay evidence +intersection_of: ECO:0001060 ! in vitro deacetylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:23:42Z + +[Term] +id: ECO:0001193 +name: in vitro defarnesylation assay evidence used in manual assertion +def: "A type of in vitro defarnesylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001061 ! in vitro defarnesylation assay evidence +intersection_of: ECO:0001061 ! in vitro defarnesylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:25:45Z + +[Term] +id: ECO:0001194 +name: in vitro demethylation assay evidence used in manual assertion +def: "A type of in vitro demethylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001062 ! in vitro demethylation assay evidence +intersection_of: ECO:0001062 ! in vitro demethylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:27:00Z + +[Term] +id: ECO:0001195 +name: in vitro desumoylation assay evidence used in manual assertion +def: "A type of in vitro desumoylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001063 ! in vitro desumoylation assay evidence +intersection_of: ECO:0001063 ! in vitro desumoylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:29:01Z + +[Term] +id: ECO:0001196 +name: in vitro deubiquitination assay evidence used in manual assertion +def: "A type of in vitro deubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001059 ! in vitro deubiquitination assay evidence +intersection_of: ECO:0001059 ! in vitro deubiquitination assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:29:48Z + +[Term] +id: ECO:0001197 +name: in vitro farnesylation assay evidence used in manual assertion +def: "A type of in vitro farnesylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001064 ! in vitro farnesylation assay evidence +intersection_of: ECO:0001064 ! in vitro farnesylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:30:50Z + +[Term] +id: ECO:0001198 +name: in vitro methylation assay evidence used in manual assertion +def: "A type of in vitro methylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001065 ! in vitro methylation assay evidence +intersection_of: ECO:0001065 ! in vitro methylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:31:54Z + +[Term] +id: ECO:0001199 +name: in vitro palmitoylation assay evidence used in manual assertion +def: "A type of in vitro palmitoylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001066 ! in vitro palmitoylation assay evidence +intersection_of: ECO:0001066 ! in vitro palmitoylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:32:57Z + +[Term] +id: ECO:0001200 +name: in vitro phosphatase assay evidence used in manual assertion +def: "A type of in vitro phosphatase assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001067 ! in vitro phosphatase assay evidence +intersection_of: ECO:0001067 ! in vitro phosphatase assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:34:16Z + +[Term] +id: ECO:0001201 +name: in vitro polyADP-ribosylation assay evidence used in manual assertion +def: "A type of in vitro polyADP-ribosylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001069 ! in vitro polyADP-ribosylation assay evidence +intersection_of: ECO:0001069 ! in vitro polyADP-ribosylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:34:57Z + +[Term] +id: ECO:0001202 +name: in vitro protein kinase assay evidence used in manual assertion +def: "A type of in vitro protein kinase assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001068 ! in vitro protein kinase assay evidence +is_a: ECO:0005801 {is_inferred="true"} ! enzymatic activity assay evidence used in manual assertion +intersection_of: ECO:0001068 ! in vitro protein kinase assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:39:57Z + +[Term] +id: ECO:0001203 +name: in vitro sumoylation assay evidence used in manual assertion +def: "A type of in vitro sumoylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001070 ! in vitro sumoylation assay evidence +intersection_of: ECO:0001070 ! in vitro sumoylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:41:25Z + +[Term] +id: ECO:0001204 +name: in vitro transcription assay evidence used in manual assertion +def: "A type of in vitro transcription assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001071 ! in vitro transcription assay evidence +intersection_of: ECO:0001071 ! in vitro transcription assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:44:40Z + +[Term] +id: ECO:0001205 +name: in vitro translation assay evidence used in manual assertion +def: "A type of in vitro translation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001072 ! in vitro translation assay evidence +intersection_of: ECO:0001072 ! in vitro translation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:46:30Z + +[Term] +id: ECO:0001206 +name: in vitro ubiquitination assay evidence used in manual assertion +def: "A type of in vitro ubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001073 ! in vitro ubiquitination assay evidence +intersection_of: ECO:0001073 ! in vitro ubiquitination assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:47:18Z + +[Term] +id: ECO:0001207 +name: in vivo acetylation assay evidence used in manual assertion +def: "A type of in vivo acetylation assay evidence that is used in manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001074 ! in vivo acetylation assay evidence +intersection_of: ECO:0001074 ! in vivo acetylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:48:56Z + +[Term] +id: ECO:0001208 +name: in vivo cleavage assay evidence used in manual assertion +def: "A type of in vivo cleavage assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001075 ! in vivo cleavage assay evidence +intersection_of: ECO:0001075 ! in vivo cleavage assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:49:43Z + +[Term] +id: ECO:0001209 +name: in vivo deacetylation assay evidence used in manual assertion +def: "A type of in vivo deacetylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001076 ! in vivo deacetylation assay evidence +intersection_of: ECO:0001076 ! in vivo deacetylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:50:47Z + +[Term] +id: ECO:0001210 +name: in vivo defarnesylation assay evidence used in manual assertion +def: "A type of in vivo defarnesylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001077 ! in vivo defarnesylation assay evidence +intersection_of: ECO:0001077 ! in vivo defarnesylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:52:41Z + +[Term] +id: ECO:0001211 +name: in vivo demethylation assay evidence used in manual assertion +def: "A type of in vivo demethylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001078 ! in vivo demethylation assay evidence +intersection_of: ECO:0001078 ! in vivo demethylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:53:40Z + +[Term] +id: ECO:0001212 +name: in vivo desumoylation assay evidence used in manual assertion +def: "A type of in vivo desumoylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001080 ! in vivo desumoylation assay evidence +intersection_of: ECO:0001080 ! in vivo desumoylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:54:36Z + +[Term] +id: ECO:0001213 +name: in vivo deubiquitination assay evidence used in manual assertion +def: "A type of in vivo deubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001079 ! in vivo deubiquitination assay evidence +intersection_of: ECO:0001079 ! in vivo deubiquitination assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:56:28Z + +[Term] +id: ECO:0001214 +name: in vivo farnesylation assay evidence used in manual assertion +def: "A type of in vivo farnesylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001081 ! in vivo farnesylation assay evidence +intersection_of: ECO:0001081 ! in vivo farnesylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T17:57:18Z + +[Term] +id: ECO:0001215 +name: in vivo methylation assay evidence used in manual assertion +def: "A type of in vivo methylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001082 ! in vivo methylation assay evidence +intersection_of: ECO:0001082 ! in vivo methylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:03:16Z + +[Term] +id: ECO:0001216 +name: in vivo palmitoylation assay evidence used in manual assertion +def: "A type of in vivo palmitoylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001083 ! in vivo palmitoylation assay evidence +intersection_of: ECO:0001083 ! in vivo palmitoylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:04:07Z + +[Term] +id: ECO:0001217 +name: in vivo phosphatase assay evidence used in manual assertion +def: "A type of in vivo phosphatase assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001084 ! in vivo phosphatase assay evidence +intersection_of: ECO:0001084 ! in vivo phosphatase assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:06:08Z + +[Term] +id: ECO:0001218 +name: in vivo protein kinase assay evidence used in manual assertion +def: "A type of in vivo protein kinase assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001085 ! in vivo protein kinase assay evidence +is_a: ECO:0005801 {is_inferred="true"} ! enzymatic activity assay evidence used in manual assertion +intersection_of: ECO:0001085 ! in vivo protein kinase assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:07:58Z + +[Term] +id: ECO:0001219 +name: in vivo sumoylation assay evidence used in manual assertion +def: "A type of in vivo sumoylation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001086 ! in vivo sumoylation assay evidence +intersection_of: ECO:0001086 ! in vivo sumoylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:09:49Z + +[Term] +id: ECO:0001220 +name: in vivo transcription assay evidence used in manual assertion +def: "A type of in vivo transcription assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001087 ! in vivo transcription assay evidence +intersection_of: ECO:0001087 ! in vivo transcription assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:11:20Z + +[Term] +id: ECO:0001221 +name: in vivo translation assay evidence used in manual assertion +def: "A type of in vivo translation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001088 ! in vivo translation assay evidence +intersection_of: ECO:0001088 ! in vivo translation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:42:50Z + +[Term] +id: ECO:0001222 +name: in vivo ubiquitination assay evidence used in manual assertion +def: "A type of in vivo ubiquitination assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001089 ! in vivo ubiquitination assay evidence +intersection_of: ECO:0001089 ! in vivo ubiquitination assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:44:11Z + +[Term] +id: ECO:0001223 +name: induced mutation evidence used in manual assertion +def: "A type of induced mutation evidence that is used in a manual assertion." [ECO:MCC] +property_value: IAO:0000234 SIB:PG xsd:string +is_obsolete: true +created_by: mchibucos +creation_date: 2014-03-17T18:45:56Z + +[Term] +id: ECO:0001224 +name: knockin evidence used in manual assertion +def: "A type of knockin evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001090 ! knockin evidence +is_a: ECO:0006049 {is_inferred="true"} ! genetic transformation evidence used in manual assertion +intersection_of: ECO:0001090 ! knockin evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:49:35Z + +[Term] +id: ECO:0001225 +name: knockout evidence used in manual assertion +def: "A type of knockout evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001091 ! knockout phenotypic evidence +is_a: ECO:0006049 {is_inferred="true"} ! genetic transformation evidence used in manual assertion +intersection_of: ECO:0001091 ! knockout phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:51:31Z + +[Term] +id: ECO:0001226 +name: lipid binding assay evidence used in manual assertion +def: "A type of lipid binding assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +is_a: ECO:0001092 ! lipid binding assay evidence +intersection_of: ECO:0001092 ! lipid binding assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:52:19Z + +[Term] +id: ECO:0001227 +name: luminescence-based mammalian interactome mapping assay evidence used in manual assertion +def: "A type of LUMIER assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "LUMIER assay evidence" BROAD [] +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +is_a: ECO:0001093 ! luminescence-based mammalian interactome mapping assay evidence +intersection_of: ECO:0001093 ! luminescence-based mammalian interactome mapping assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:55:16Z + +[Term] +id: ECO:0001228 +name: macroscopy evidence used in manual assertion +def: "A type of macroscopy evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001094 ! macroscopy evidence +intersection_of: ECO:0001094 ! macroscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:56:21Z + +[Term] +id: ECO:0001229 +name: mammalian 2-hybrid assay evidence used in manual assertion +def: "A type of mammalian 2-hybrid assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001095 ! mammalian 2-hybrid assay evidence +is_a: ECO:0006077 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in manual assertion +intersection_of: ECO:0001095 ! mammalian 2-hybrid assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:57:25Z + +[Term] +id: ECO:0001230 +name: mass spectrometry evidence used in manual assertion +def: "A type of mass spectrometry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001096 ! mass spectrometry evidence +intersection_of: ECO:0001096 ! mass spectrometry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T18:59:50Z + +[Term] +id: ECO:0001231 +name: medical imaging evidence used in manual assertion +def: "A type of medical imaging evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001097 ! medical imaging evidence +is_a: ECO:0001256 {is_inferred="true"} ! imaging assay evidence used in manual assertion +intersection_of: ECO:0001097 ! medical imaging evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:04:43Z + +[Term] +id: ECO:0001232 +name: microscopy evidence used in manual assertion +def: "A type of microscopy evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001098 ! microscopy evidence +is_a: ECO:0001256 {is_inferred="true"} ! imaging assay evidence used in manual assertion +intersection_of: ECO:0001098 ! microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:05:37Z + +[Term] +id: ECO:0001233 +name: motility wound healing assay evidence used in manual assertion +alt_id: ECO:0001262 +def: "A type of motility wound healing assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "wound healing assay evidence used in manual assertion" BROAD [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001099 ! motility wound healing assay evidence +intersection_of: ECO:0001099 ! motility wound healing assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:08:13Z + +[Term] +id: ECO:0001234 +name: MTS assay evidence used in manual assertion +def: "A type of MTS assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium" RELATED [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001100 ! MTS assay evidence +intersection_of: ECO:0001100 ! MTS assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:08:51Z + +[Term] +id: ECO:0001235 +name: MTT assay evidence used in manual assertion +def: "A type of MTT assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide" RELATED [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001101 ! MTT assay evidence +intersection_of: ECO:0001101 ! MTT assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:13:17Z + +[Term] +id: ECO:0001236 +name: multiplex bead-based immunoassay evidence used in manual assertion +def: "A type of multiplex bead-based immunoassay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001102 ! multiplex bead-based immunoassay evidence +intersection_of: ECO:0001102 ! multiplex bead-based immunoassay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:14:03Z + +[Term] +id: ECO:0001237 +name: natural variation mutant evidence used in manual assertion +def: "A type of natural variation mutant evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001103 ! natural variation mutant evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0001103 ! natural variation mutant evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:15:48Z + +[Term] +id: ECO:0001238 +name: nuclear magnetic resonance evidence used in manual assertion +def: "A type of nuclear magnetic resonance evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001105 ! nuclear magnetic resonance evidence +is_a: ECO:0006285 {is_inferred="true"} ! magnetic resonance evidence used in manual assertion +intersection_of: ECO:0001105 ! nuclear magnetic resonance evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:18:50Z + +[Term] +id: ECO:0001239 +name: nuclear fragmentation evidence used in manual assertion +def: "A type of nuclear fragmentation evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001104 ! nuclear fragmentation evidence +intersection_of: ECO:0001104 ! nuclear fragmentation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:19:55Z + +[Term] +id: ECO:0001240 +name: nuclease protection assay evidence used in manual assertion +def: "A type of nuclease protection assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001106 ! nuclease protection assay evidence +intersection_of: ECO:0001106 ! nuclease protection assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:23:41Z + +[Term] +id: ECO:0001241 +name: nucleotide analog incorporation assay evidence used in manual assertion +def: "A type of nucleotide analog incorporation assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001107 ! nucleotide analog incorporation assay evidence +intersection_of: ECO:0001107 ! nucleotide analog incorporation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:25:17Z + +[Term] +id: ECO:0001242 +name: phage display evidence used in manual assertion +def: "A type of phage display evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001108 ! phage display evidence +is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion +intersection_of: ECO:0001108 ! phage display evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:27:17Z + +[Term] +id: ECO:0001243 +name: phosphoamino acid analysis evidence used in manual assertion +def: "A type of phosphoamino acid analysis evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001109 ! phosphoamino acid analysis evidence +intersection_of: ECO:0001109 ! phosphoamino acid analysis evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:28:59Z + +[Term] +id: ECO:0001244 +name: peptide affinity enrichment evidence used in manual assertion +def: "A type of peptide affinity enrichment evidence that is used in a manual assertion." [ECO:MCC] +synonym: "phosphopeptide affinity enrichment evidence" RELATED [] +is_a: ECO:0001110 ! peptide affinity enrichment evidence +is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion +intersection_of: ECO:0001110 ! peptide affinity enrichment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T19:30:13Z + +[Term] +id: ECO:0001245 +name: peptide array evidence used in manual assertion +def: "A type of peptide array evidence that is used in a manual assertion." [ECO:MCC] +synonym: "phosphopeptide array evidence" RELATED [] +is_a: ECO:0001112 ! peptide array evidence +is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion +intersection_of: ECO:0001112 ! peptide array evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:16:45Z + +[Term] +id: ECO:0001246 +name: physical examination evidence used in manual assertion +def: "A type of physical examination evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001111 ! physical examination evidence +intersection_of: ECO:0001111 ! physical examination evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:17:27Z + +[Term] +id: ECO:0001247 +name: point mutation phenotypic evidence used in manual assertion +def: "A type of point mutation phenotypic evidence that is used in a manual assertion." [ECO:MCC] +synonym: "point mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0001113 ! point mutation phenotypic evidence +intersection_of: ECO:0001113 ! point mutation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:18:10Z + +[Term] +id: ECO:0001248 +name: propidium iodide staining evidence used in manual assertion +def: "A type of propidium iodide staining evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001114 ! propidium iodide staining evidence +intersection_of: ECO:0001114 ! propidium iodide staining evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:18:58Z + +[Term] +id: ECO:0001249 +name: fluorescence evidence used in manual assertion +def: "A type of protein detection by fluorescence evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001115 ! fluorescence evidence +intersection_of: ECO:0001115 ! fluorescence evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:19:35Z + +[Term] +id: ECO:0001250 +name: protein dot blot assay evidence used in manual assertion +def: "A type of protein dot blot assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001116 ! protein dot blot assay evidence +intersection_of: ECO:0001116 ! protein dot blot assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:20:30Z + +[Term] +id: ECO:0001251 +name: protein microarray evidence used in manual assertion +def: "A type of protein microarray evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001117 ! protein microarray evidence +intersection_of: ECO:0001117 ! protein microarray evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:21:11Z + +[Term] +id: ECO:0001252 +name: protein sequencing assay evidence used in manual assertion +def: "A type of protein sequencing assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001118 ! protein sequencing assay evidence +intersection_of: ECO:0001118 ! protein sequencing assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:22:08Z + +[Term] +id: ECO:0001253 +name: quantitative mass spectrometry evidence used in manual assertion +def: "A type of quantitative mass spectrometry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001119 ! quantitative mass spectrometry evidence +is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion +intersection_of: ECO:0001119 ! quantitative mass spectrometry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:23:15Z + +[Term] +id: ECO:0001254 +name: radioisotope assay evidence used in manual assertion +def: "A type of radioisotope assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "radioassay evidence" BROAD [] +synonym: "radioisotopic assay evidence" BROAD [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001120 ! radioisotope assay evidence +intersection_of: ECO:0001120 ! radioisotope assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:27:05Z + +[Term] +id: ECO:0001255 +name: radioimmunoassay evidence used in manual assertion +def: "A type of radioimmunoassay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001121 ! radioimmunoassay evidence +intersection_of: ECO:0001121 ! radioimmunoassay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:28:04Z + +[Term] +id: ECO:0001256 +name: imaging assay evidence used in manual assertion +def: "A type of imaging assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "radiologic test evidence" RELATED [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0000324 ! imaging assay evidence +intersection_of: ECO:0000324 ! imaging assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:00Z + +[Term] +id: ECO:0001257 +name: restriction fragment detection evidence used in manual assertion +def: "A type of restriction fragment detection evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001124 ! restriction fragment detection evidence +intersection_of: ECO:0001124 ! restriction fragment detection evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:05Z + +[Term] +id: ECO:0001258 +name: spectrophotometry evidence used in manual assertion +def: "A type of spectrophotometry evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001126 ! spectrophotometry evidence +intersection_of: ECO:0001126 ! spectrophotometry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:09Z + +[Term] +id: ECO:0001259 +name: syngeneic transplantation experiment evidence used in manual assertion +def: "A type of syngeneic transplantation experiment evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001128 ! syngeneic transplantation experiment evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0001128 ! syngeneic transplantation experiment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:13Z + +[Term] +id: ECO:0001260 +name: xenotransplantation phenotypic evidence used in manual assertion +def: "A type of xenotransplantation phenotypic evidence that is used in a manual assertion." [ECO:MCC] +synonym: "tissue grafting" RELATED [] +synonym: "xenograft transplantation evidence" BROAD [] +synonym: "xenografting evidence" BROAD [] +synonym: "xenotransplantation evidence" BROAD [] +synonym: "xenotransplantation experiment evidence used in manual assertion" EXACT [] +is_a: ECO:0001137 ! xenotransplantation phenotypic evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0001137 ! xenotransplantation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:18Z + +[Term] +id: ECO:0001261 +name: WST-1 assay evidence used in manual assertion +def: "A type of WST-1 assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "metabolic cell proliferation assay evidence" BROAD [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001136 ! WST-1 assay evidence +intersection_of: ECO:0001136 ! WST-1 assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:22Z + +[Term] +id: ECO:0001263 +name: urine test evidence used in manual assertion +def: "A type of urine test evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001134 ! urine test evidence +intersection_of: ECO:0001134 ! urine test evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:30Z + +[Term] +id: ECO:0001264 +name: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion +def: "A type of terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "TdT-mediated dUTP-biotin nick end labeling assay evidence" BROAD [] +synonym: "terminal deoxynucleotidyl transferase-dUTP nick end labeling assay evidence" BROAD [] +synonym: "TUNEL assay evidence" BROAD [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001133 ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence +intersection_of: ECO:0001133 ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:34Z + +[Term] +id: ECO:0001265 +name: tryptic phosphopeptide mapping assay evidence used in manual assertion +def: "A type of tryptic phosphopeptide mapping assay evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001132 ! tryptic phosphopeptide mapping assay evidence +is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion +intersection_of: ECO:0001132 ! tryptic phosphopeptide mapping assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:38Z + +[Term] +id: ECO:0001266 +name: transgenic organism evidence used in manual assertion +def: "A type of transgenic organism evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001131 ! transgenic organism evidence +is_a: ECO:0006049 {is_inferred="true"} ! genetic transformation evidence used in manual assertion +intersection_of: ECO:0001131 ! transgenic organism evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:41Z + +[Term] +id: ECO:0001267 +name: tissue microarray evidence used in manual assertion +def: "A type of tissue microarray evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0001130 ! tissue microarray evidence +is_a: ECO:0001172 {is_inferred="true"} ! cytochemistry evidence used in manual assertion +intersection_of: ECO:0001130 ! tissue microarray evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:45Z + +[Term] +id: ECO:0001268 +name: TACE activity assay evidence used in manual assertion +def: "A type of TACE activity assay evidence that is used in a manual assertion." [ECO:MCC] +synonym: "Tumor necrosis factor - alpha converting enzyme activity assay evidence" BROAD [] +is_a: ECO:0001129 ! TACE activity assay evidence +is_a: ECO:0005801 {is_inferred="true"} ! enzymatic activity assay evidence used in manual assertion +intersection_of: ECO:0001129 ! TACE activity assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:49Z + +[Term] +id: ECO:0001269 +name: surface plasmon resonance evidence used in manual assertion +def: "A type of surface plasmon resonance evidence that is used in a manual assertion." [ECO:MCC] +synonym: "SPR evidence" BROAD [] +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +is_a: ECO:0001127 ! surface plasmon resonance evidence +intersection_of: ECO:0001127 ! surface plasmon resonance evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:54Z + +[Term] +id: ECO:0001270 +name: restriction landmark genomic scanning evidence used in manual assertion +def: "A type of restriction landmark genome scanning evidence that is used in a manual assertion." [ECO:MCC] +synonym: "restriction landmark genome scanning evidence" BROAD [] +synonym: "RLGS evidence" BROAD [] +is_a: ECO:0000089 ! restriction landmark genomic scanning evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000089 ! restriction landmark genomic scanning evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:29:58Z + +[Term] +id: ECO:0001271 +name: resonant mirror biosensor evidence used in manual assertion +def: "A type of resonant mirror biosensor evidence that is used in a manual assertion." [ECO:MCC] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001123 ! resonant mirror biosensor evidence +intersection_of: ECO:0001123 ! resonant mirror biosensor evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T20:30:02Z + +[Term] +id: ECO:0001272 +name: high-performance liquid chromatography evidence used in manual assertion +def: "A type of high-performance liquid chromatography evidence that is used in a manual assertion." [ECO:MCC] +synonym: "HPLC evidence" BROAD [] +is_a: ECO:0001052 ! high-performance liquid chromatography evidence +is_a: ECO:0007680 {is_inferred="true"} ! chromatography evidence used in manual assertion +intersection_of: ECO:0001052 ! high-performance liquid chromatography evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: mchibucos +creation_date: 2014-03-16T20:49:54Z + +[Term] +id: ECO:0001273 +name: ectopic expression evidence used in manual assertion +def: "A type of ectopic expression evidence that is used in a manual assertion." [ECO:MCC] +synonym: "analysis of overexpression/ectopic expression phenotype" RELATED [] +is_a: ECO:0000017 ! ectopic expression evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0000017 ! ectopic expression evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: mchibucos +creation_date: 2014-03-17T22:34:56Z + +[Term] +id: ECO:0001522 +name: small molecule detection assay evidence +def: "A type of molecule detection assay evidence resulting from the detection and quantification of a small molecule (a low molecular weight (typically < 900 daltons) organic compound), such as lipids, sugars, animo acids, drugs, etc." [] +synonym: "metabolite detection assay evidence" NARROW [] +is_a: ECO:0005516 ! molecule detection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro + +[Term] +id: ECO:0001533 +name: localization evidence +def: "A type of direct assay evidence in which the sub-cellular location of a protein or nucleic acid sequence is determined." [] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_ExpGenomicCleanup" xsd:string +created_by: jbmunro +creation_date: 2018-05-17T12:00:00Z + +[Term] +id: ECO:0001534 +name: nucleic acid localization evidence +def: "A type of localization evidence based on localization of a specific segment of DNA or RNA within tissue by the application of a complementary strand of nucleic acid to which a reporter molecule (i.e. either radio-, fluorescent- or antigen-labeled probe) is attached and quantified using either autoradiography, fluorescence microscopy, or immunohistochemistry." [] +is_a: ECO:0001533 ! localization evidence +property_value: IAO:0000234 "ECO_ExpGenomicCleanup" xsd:string +created_by: jbmunro +creation_date: 2018-05-17T12:00:00Z + +[Term] +id: ECO:0001546 +name: acetylation assay evidence +def: "A type of direct assay evidence where acetylated residues are detected in a protein." [SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001547 +name: cleavage assay evidence +def: "A type of direct assay evidence where the cleavage of a protein into protein fragments by a protease is detected." [PMID:21121091, PMID:22154596] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001548 +name: deacetylation assay evidence +def: "A type of direct assay evidence where the removal of acetyl groups are detected in a protein." [SIB:PG, url:http\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001549 +name: defarnesylation assay evidence +def: "A type of direct assay evidence where the removal of farnesyl groups from a protein is detected." [PMID:15556768, PMID:16126733, PMID:16507103, SIB:PG] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001550 +name: demethylation assay evidence +def: "A type of direct assay evidence where the removal of methyl groups from a substrate (RNA/DNA or protein) is detected." [SIB:PG, url:http\://en.wikipedia.org/wiki/Demethylation] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001551 +name: desumoylation assay evidence +def: "A type of direct assay evidence where the removal of sumo groups from a protein is detected." [SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001552 +name: deubiquitination assay evidence +def: "A type of direct assay evidence where the removal of ubiquitin groups from a protein is detected." [PMID:19692941, SIB:PG] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001553 +name: farnesylation assay evidence +def: "A type of direct assay evidence where farnesylated residues in proteins are detected." [PMID:9030603, SIB:PG, url:http\://en.wikipedia.org/wiki/Farnesyltransferase] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001554 +name: methylation assay evidence +def: "A type of direct assay evidence where methylated residues of a substarte (RNA/DNA or protein) are detected." [SIB:PG, url:http\://en.wikipedia.org/wiki/Methylation] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001555 +name: palmitoylation assay evidence +def: "A type of direct assay evidence where palmitoylated residues in a protein are detected." [PMID:10329400, PMID:17077383, SIB:PG] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001556 +name: phosphatase assay evidence +def: "A type of direct assay evidence where the removal of phosphatase groups from a protein is detected." [SIB:PG, url:http\://en.wikipedia.org/wiki/Phosphatase] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001557 +name: polyADP-ribosylation assay evidence +def: "A type of direct assay evidence where ADP-ribosylated residues in proteins are detected." [PMID:21870253, PMID:2820766, SIB:PG] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001558 +name: protein kinase assay evidence +def: "A type of enzymatic activity assay evidence that measures transfer of a phosphate to a peptide or protein substrate by a protein kinase." [url:https\://www.ncbi.nlm.nih.gov/books/NBK91991/] +is_a: ECO:0000005 ! enzymatic activity assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: jbmunro +created_by: mchibucos +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001559 +name: sumoylation assay evidence +def: "A type of direct assay evidence where sumoylated residues on a protein are detected." [SIB:PG, url:http\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:000156 +name: single-cell RNA-sequencing evidence used in automatic assertion +def: "A type of single cell RNA-sequencing evidence that is used in automatic assertion." [] +is_a: ECO:0001560 ! single-cell RNA-sequencing evidence +is_a: ECO:0006069 {is_inferred="true"} ! RNA-sequencing evidence used in automatic assertion +intersection_of: ECO:0001560 ! single-cell RNA-sequencing evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: jbmunro +creation_date: 2019-01-02T12:00:00Z + +[Term] +id: ECO:0001560 +name: single-cell RNA-sequencing evidence +def: "A type of RNA-sequencing evidence that uses a single cell as the source of the RNA." [] +synonym: "scRNA-seq evidence" EXACT [] +is_a: ECO:0000295 ! RNA-sequencing evidence +property_value: IAO:0000234 "Bgee" xsd:string +created_by: jbmunro +creation_date: 2019-01-02T12:00:00Z + +[Term] +id: ECO:0001561 +name: translation assay evidence +def: "A type of direct assay evidence where de novo protein synthesis is detected." [PMID:18230759, PMID:24901308, SIB:PG] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001562 +name: ubiquitination assay evidence +def: "A type of direct assay evidence where ubiquitinated residues on a protein are detected." [PMID:19692941, SIB:PG] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001563 +name: cell growth assay evidence +def: "A type of direct assay evidence where biological cell development, i.e. an increase in cell mass and size are measured." [GO:0016049, PMID:11057898] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000112 "However, as shown in Fig. 5A, the recombinant mycobacterial cells became sensitive to the anti-TB drugs isoniazid and streptomycin, as evidenced by their inhibited growth in the presence of 25 mug/mL of isoniazid or 0.5 mug/mL of streptomycin in the medium." xsd:string {xref="PMID:20843371"} +property_value: IAO:0000112 "Similar growth profiles of these strains were observed in liquid media with the corresponding inducers (data not shown). These indicate that the expression of vapC10 alone led to growth arrest of E. coli, and the simultaneous expression of vapB10 could neutralize this growth-inhibition effect, suggesting that vapC10 encodes a TA toxin and vapB10 encodes the cognate antitoxin." xsd:string {xref="PMID:24260461"} +property_value: IAO:0000234 "ECO_AssayCleanup" xsd:string +created_by: mchibucos / jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001565 +name: cell-based assay evidence +def: "A type of direct assay evidence resulting from the study of cells." [] +is_a: ECO:0000002 ! direct assay evidence +created_by: jbmunro +creation_date: 2017-11-30T14:00:00Z + +[Term] +id: ECO:0001566 +name: quantitative reverse transcription polymerase chain reaction evidence +def: "A type of reverse transcription polymerase chain reaction evidence that combines reverse transcription polymerase chain reaction and real time polymerase chain reaction to quantitatively assay for the detection of RNA levels." [] +synonym: "qRT-PCR evidence" EXACT [] +synonym: "quantitative real-time RT-PCR evidence" EXACT [] +synonym: "quantitative RT-PCR evidence" EXACT [] +synonym: "real-time qRT-PCR evidence" EXACT [] +synonym: "real-time quantitative reverse transcription PCR evidence" EXACT [] +synonym: "real-time reverse transcription polymerase chain reaction evidence" EXACT [] +synonym: "real-time RT-PCR evidence" EXACT [] +synonym: "RRT-PCR evidence" EXACT [] +synonym: "rRT-PCR evidence" EXACT [] +synonym: "RT-qPCR evidence" EXACT [] +is_a: ECO:0000109 ! reverse transcription polymerase chain reaction evidence +property_value: IAO:0000112 "The real-time RT-PCR validated that Zur repressed the first gene of each of the three operons, znuA, znuCB and ykgM-rpmJ2 (Additional file 5)." xsd:string {xref="PMID:19552825"} +created_by: jbmunro +creation_date: 2018-07-07T12:00:00Z + +[Term] +id: ECO:0001567 +name: quantitative reverse transcription polymerase chain reaction evidence used in manual assertion +def: "A type of quantitative reverse transcription polymerase chain reaction evidence used in manual assertion." [] +is_a: ECO:0001566 ! quantitative reverse transcription polymerase chain reaction evidence +is_a: ECO:0001808 {is_inferred="true"} ! reverse transcription polymerase chain reaction evidence used in manual assertion +intersection_of: ECO:0001566 ! quantitative reverse transcription polymerase chain reaction evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: jbmunro +creation_date: 2018-07-07T12:00:00Z + +[Term] +id: ECO:0001568 +name: quantitative reverse transcription polymerase chain reaction evidence used in automatic assertion. +alt_id: ECO:000156 +def: "A type of quantitative reverse transcription polymerase chain reaction evidence used in automatic assertion." [] +is_a: ECO:0001566 ! quantitative reverse transcription polymerase chain reaction evidence +is_a: ECO:0007475 {is_inferred="true"} ! reverse transcription polymerase chain reaction evidence used in automatic assertion +intersection_of: ECO:0001566 ! quantitative reverse transcription polymerase chain reaction evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: jbmunro +creation_date: 2018-07-07T12:00:00Z + +[Term] +id: ECO:000157 +name: colony diameter phenotype evidence used in manual assertion +def: "A type of colony diameter phenotype evidence that is used in manual assertion." [] +is_a: ECO:0001571 ! colony diameter phenotype evidence +is_a: ECO:0007082 {is_inferred="true"} ! colony size phenotypic evidence used in manual assertion +intersection_of: ECO:0001571 ! colony diameter phenotype evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: jbmunro +creation_date: 2019-01-03T12:00:00Z + +[Term] +id: ECO:0001570 +name: single-cell RNA-sequencing evidence used in manual assertion +def: "A type of single cell RNA-sequencing evidence that is used in manual assertion." [] +is_a: ECO:0001560 ! single-cell RNA-sequencing evidence +is_a: ECO:0006068 {is_inferred="true"} ! RNA-sequencing evidence used in manual assertion +intersection_of: ECO:0001560 ! single-cell RNA-sequencing evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: jbmunro +creation_date: 2019-01-02T12:00:00Z + +[Term] +id: ECO:0001571 +name: colony diameter phenotype evidence +def: "A type of colony size phenotypic evidence resulting from measurement of a microbial colony's diameter." [] +is_a: ECO:0007081 ! colony size phenotypic evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-01-03T12:00:00Z + +[Term] +id: ECO:0001572 +name: colony diameter phenotype evidence used in automatic assertion +def: "A type of colony diameter phenotype evidence that is used in automatic assertion" [] +is_a: ECO:0001571 ! colony diameter phenotype evidence +is_a: ECO:0007627 {is_inferred="true"} ! colony size phenotypic evidence used in automatic assertion +intersection_of: ECO:0001571 ! colony diameter phenotype evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: jbmunro +creation_date: 2019-01-03T12:00:00Z + +[Term] +id: ECO:0001574 +name: membrane fusion assay evidence +def: "A type of direct assay evidence in which a broad variety of physiological processes are measured when two separate membranes merge into a single contiguous membrane." [PMID:1836993] +comment: Measurements can include but are not limited to: synaptic transmission, fertilization, and viral entry. +is_a: ECO:0000002 ! direct assay evidence +created_by: jbmunro +creation_date: 2019-03-01T12:00:00Z + +[Term] +id: ECO:0001575 +name: membrane fusion assay evidence used in automatic assertion +def: "A type of membrane fusion assay evidence that is used in automatic assertion." [] +is_a: ECO:0001574 {is_inferred="true"} ! membrane fusion assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001574 ! membrane fusion assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: jbmunro +creation_date: 2019-03-01T12:00:00Z + +[Term] +id: ECO:0001576 +name: membrane fusion assay evidence used in manual assertion +def: "A type of membrane fusion assay evidence that is used in manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001574 {is_inferred="true"} ! membrane fusion assay evidence +intersection_of: ECO:0001574 ! membrane fusion assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: jbmunro +creation_date: 2019-03-01T12:00:00Z + +[Term] +id: ECO:0001577 +name: spheroplast fusion assay evidence +def: "A type of membrane fusion assay evidence based on the fusion of spheroplasts." [] +comment: A spheroplast is a Gram-negative bacterium cell in which the cell wall has been almost completely removed. {xref="PMID:25870259"} +is_a: ECO:0001574 ! membrane fusion assay evidence +created_by: jbmunro +creation_date: 2019-03-01T12:00:00Z + +[Term] +id: ECO:0001578 +name: spheroplast fusion assay evidence used in automatic assertion +def: "A type of spheroplast fusion assay evidence used in automatic assertion." [] +is_a: ECO:0001575 {is_inferred="true"} ! membrane fusion assay evidence used in automatic assertion +is_a: ECO:0001577 {is_inferred="true"} ! spheroplast fusion assay evidence +intersection_of: ECO:0001577 ! spheroplast fusion assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: jbmunro +creation_date: 2019-03-01T12:00:00Z + +[Term] +id: ECO:0001579 +name: spheroplast fusion assay evidence used in manual assertion +def: "A type of spheroplast fusion assay evidence that is used in manual assertion." [] +is_a: ECO:0001576 {is_inferred="true"} ! membrane fusion assay evidence used in manual assertion +is_a: ECO:0001577 {is_inferred="true"} ! spheroplast fusion assay evidence +intersection_of: ECO:0001577 ! spheroplast fusion assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: jbmunro +creation_date: 2019-03-01T12:00:00Z + +[Term] +id: ECO:0001580 +name: liquid chromatography coupled with tandem mass spectrometry evidence +def: "A type of mass spectrometry evidence where liquid chromatography is used to separate particles in solution, followed by fragmentation and measurement of the mass-to-charge ratio of the resulting particles to identify the amount and type of material entities present in a sample." [] +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-03-26T12:00:00Z + +[Term] +id: ECO:0001581 +name: liquid chromatography coupled with tandem mass spectrometry evidence used in automatic assertion +def: "A type of liquid chromatography coupled with tandem mass spectrometry evidence that is used in an automatic assertion." [] +is_a: ECO:0001580 {is_inferred="true"} ! liquid chromatography coupled with tandem mass spectrometry evidence +is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion +intersection_of: ECO:0001580 ! liquid chromatography coupled with tandem mass spectrometry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-03-26T12:00:00Z + +[Term] +id: ECO:0001582 +name: liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion +def: "A type of liquid chromatography coupled with tandem mass spectrometry evidence that is used in a manual assertion." [] +is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion +is_a: ECO:0001580 {is_inferred="true"} ! liquid chromatography coupled with tandem mass spectrometry evidence +intersection_of: ECO:0001580 ! liquid chromatography coupled with tandem mass spectrometry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-03-26T12:00:00Z + +[Term] +id: ECO:0001583 +name: small interfering RNA knockdown evidence +def: "A type of anti-sense experiment evidence where gene expression is disrupted through the introduction of double-stranded RNA molecules, 20-25 base pairs in length, which operate within the RNA interference pathway." [] +synonym: "short interfering RNA" EXACT [] +synonym: "silencing RNA" EXACT [] +synonym: "siRNA" EXACT [] +is_a: ECO:0000018 ! anti-sense experiment evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-03T12:00:00Z + +[Term] +id: ECO:0001584 +name: small interfering RNA knockdown evidence used in automatic assertion +def: "A type of small interfering RNA knockdown evidence that is used in an automatic assertion." [] +is_a: ECO:0001583 ! small interfering RNA knockdown evidence +is_a: ECO:0007556 {is_inferred="true"} ! anti-sense experiment evidence used in automatic assertion +intersection_of: ECO:0001583 ! small interfering RNA knockdown evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-03T12:00:00Z + +[Term] +id: ECO:0001585 +name: small interfering RNA knockdown evidence used in manual assertion +def: "A type of small interfering RNA knockdown evidence that is used in a manual assertion." [] +is_a: ECO:0001583 ! small interfering RNA knockdown evidence +is_a: ECO:0006050 {is_inferred="true"} ! anti-sense experiment evidence used in manual assertion +intersection_of: ECO:0001583 ! small interfering RNA knockdown evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-03T12:00:00Z + +[Term] +id: ECO:0001586 +name: ion trap mass spectrometry evidence +def: "A type of mass spectrometry evidence where a combination of magnetic and/or electric fields are utilized to capture charged particles in tandem with mass spectrometry." [PMID:1561330] +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001587 +name: ion trap mass spectrometry evidence used in automatic assertion +def: "A type of ion trap mass spectrometry evidence that is used in an automatic assertion." [] +is_a: ECO:0001586 ! ion trap mass spectrometry evidence +is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion +intersection_of: ECO:0001586 ! ion trap mass spectrometry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001588 +name: ion trap mass spectrometry evidence used in manual assertion +def: "A type of ion trap mass spectrometry evidence that is used in a manual assertion." [] +is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion +is_a: ECO:0001586 ! ion trap mass spectrometry evidence +intersection_of: ECO:0001586 ! ion trap mass spectrometry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001589 +name: atomic force microscopy evidence +def: "A type of microscopy evidence where a mechanical probe is applied to a sample (i.e. a type of scanning probe microscopy) which can result in production of an image (with resolution in the order of fractions of a nanometer), force measurement, or sample manipulation." [] +comment: Atomic force microscopy can be used for force measurement (force spectroscopy), imaging (rendering a three-dimensional shape (topography), and manipulation of the sample. +synonym: "AFM" EXACT [] +synonym: "scanning force microscopy" EXACT [] +synonym: "SFM" EXACT [] +is_a: ECO:0001098 ! microscopy evidence +property_value: IAO:0000112 "AFM revealed loop structures stabilized by multiple EspR dimer of dimers suggesting the presence of several distant EspR binding sites in the espACD upstream region." xsd:string {xref="PMID:22479184"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001590 +name: atomic force microscopy evidence used in automatic assertion +def: "A type of atomic force microscopy evidence that is used in an automatic assertion." [] +is_a: ECO:0001589 ! atomic force microscopy evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001591 +name: atomic force microscopy evidence used in manual assertion +def: "A type of atomic force microscopy evidence that is used in a manual assertion." [] +is_a: ECO:0001589 ! atomic force microscopy evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001592 +name: polysome profiling evidence +def: "A type of experimental evidence employed to study the associations between multiple ribosomes and mRNA that utilizes density gradient centrifugation to separate out the lysate of cells of interest with the objective of isolating polysomes/polyribosomes which are complexes of an mRNA molecule and two or more ribosomes that act to translate mRNA. The optical density of the fractions is then determined." [PMID:30545912] +comment: Once isolated, gel electrophoresis, immunoblot, and other protein profiling techniques can be applied to garner more information. +is_a: ECO:0000006 ! experimental evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001593 +name: polysome profiling evidence used in automatic assertion +def: "A type of polysome profiling evidence that is used in an automatic assertion." [] +is_a: ECO:0001592 ! polysome profiling evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0001592 ! polysome profiling evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001594 +name: polysome profiling evidence used in manual assertion +def: "A type of polysome profiling evidence that is used in a manual assertion." [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001592 ! polysome profiling evidence +intersection_of: ECO:0001592 ! polysome profiling evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: jbmunro +creation_date: 2019-04-15T12:00:00Z + +[Term] +id: ECO:0001598 +name: multilocus sequence typing evidence +def: "A type of nucleotide sequencing assay evidence in which DNA sequence variations within a specific set of genes (typically housekeeping genes) are used to characterizes strains by their unique allelic profiles." [PMID:9501229] +comment: See PMID:24713082 for use example. +synonym: "MLST" EXACT [] +is_a: ECO:0000219 ! nucleotide sequencing assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001599 +name: multilocus sequence typing evidence used in automatic assertion +def: "A type of multilocus sequence typing evidence that is used in an automatic assertion." [] +is_a: ECO:0001598 ! multilocus sequence typing evidence +is_a: ECO:0007813 {is_inferred="true"} ! nucleotide sequencing assay evidence used in automatic assertion +intersection_of: ECO:0001598 ! multilocus sequence typing evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001600 +name: multilocus sequence typing evidence used in manual assertion +def: "A type of multilocus sequence typing evidence that is used in a manual assertion" [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001598 ! multilocus sequence typing evidence +intersection_of: ECO:0001598 ! multilocus sequence typing evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001601 +name: protein-oligonucleotide microarray binding evidence +def: "A type of protein-binding evidence that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites." [PMID:22146299] +synonym: "PBM evidence" EXACT [] +is_a: ECO:0000024 ! protein binding evidence +is_a: ECO:0000136 ! nucleic acid binding evidence +created_by: swolfish +creation_date: 2015-06-05T14:36:00Z + +[Term] +id: ECO:0001603 +name: cell staining evidence +def: "A type of cell-based assay evidence in which living cells are stained with a vital stain, i.e. dye that can be used on living cells without causing cell death." [GOC:MAH] +synonym: "vital staining evidence" RELATED [GOC:MAH] +is_a: ECO:0001565 ! cell-based assay evidence +created_by: swolfish +creation_date: 2015-06-08T11:51:37Z + +[Term] +id: ECO:0001801 +name: alkaline phosphatase reporter gene assay evidence +def: "A type of reporter gene assay evidence based on the fusion of selected genes with the phoA gene to express alkaline phosphatase in periplasmic space for protein tracing." [ECO:SW, PMID:11823238] +comment: Alkaline phosphatase reporter assay produces a hybrid protein with alkaline phosphatase activity following transportation across the cellular membrane. +synonym: "SEAP reporter assay" RELATED [] +synonym: "Secreted Embryonic Alkaline Phosphatase reporter assay" RELATED [] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-10-29T15:53:45Z + +[Term] +id: ECO:0001802 +name: beta-galactosidase reporter gene assay evidence +alt_id: ECO:0000297 +def: "A type of reporter gene assay evidence based on the fusion of the lacZ gene to a specific promoter for the expression of beta-galactosidase which will appear blue when grown on a X-gal medium." [ECO:SW, PMID:20439410] +comment: The assay is often performed using a plasmid borne construction on a lacZ strain. +synonym: "beta-gal reporter gene assay" EXACT [] +synonym: "beta-galactosidase assay evidence" EXACT [] +synonym: "lacZ reporter gene assay" RELATED [] +synonym: "LacZ transcript localization evidence" EXACT [] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000112 "For each of the three genes, there was a significant increase of beta-galactosidase activity in Deltazur compared to WT when they grew in TMH with the addition of zinc. Thus, Zur repressed the promoter activities of znuC, znuA and ykgM." xsd:string {xref="PMID:19552825"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-10-29T15:57:10Z + +[Term] +id: ECO:0001803 +name: chloramphenicol acetyltransferase reporter gene assay evidence +def: "A type of reporter gene assay evidence based on the fusion of the CAT gene to a specific promoter for the expression of chloramphenicol acetyltransferase which confers resistance to the chloramphenicol antibiotic." [ECO:SW, PMID:1630936] +comment: The amount of acetylated chloramphenicol is directly proportional to the amount of CAT enzyme present. +synonym: "CAT reporter gene assay" EXACT [PMID:1630936] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-10-29T16:06:42Z + +[Term] +id: ECO:0001804 +name: beta-glucuronidase reporter gene assay evidence +def: "A type of reporter gene assay evidence where the beta-glucuronidase enzyme from Escherichia coli is used as the reporter to transform non-fluorescent substrates into fluorescents for detection." [ECO:SW] +synonym: "GUS reporter gene assay" EXACT [] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-10-29T16:11:02Z + +[Term] +id: ECO:0001805 +name: luciferase reporter gene assay evidence +def: "A type of reporter gene assay evidence where luciferase, an oxidative enzyme, is used as the reporter to detect a gene product with bioluminescence." [ECO:SW, PMID:17623934] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-10-29T16:15:39Z + +[Term] +id: ECO:0001806 +name: chromatin immunoprecipitation- exonuclease evidence +def: "A type of chromatin immunoprecipitation evidence that uses gama-exonuclease to digest TF-unbound DNA after ChIP for the identification of transcription factor binding site locations with high-resolution data." [ECO:SW, PMID:25249628] +synonym: "ChIP-exo" EXACT [PMID:25249628] +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-10-29T16:37:36Z + +[Term] +id: ECO:0001807 +name: electrophoretic mobility shift assay evidence used in manual assertion +def: "A type of electrophoretic mobility shift that is used in a manual assertion." [ECO:SW] +synonym: "electrophoretic mobility shift assay" BROAD [] +synonym: "EMSA evidence" BROAD [] +synonym: "gel retardation assay" RELATED [] +is_a: ECO:0000096 ! electrophoretic mobility shift assay evidence +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +intersection_of: ECO:0000096 ! electrophoretic mobility shift assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: swolfish +creation_date: 2015-11-03T12:13:17Z + +[Term] +id: ECO:0001808 +name: reverse transcription polymerase chain reaction evidence used in manual assertion +def: "A type of reverse transcription polymerase chain reaction evidence that is used in a manual assertion." [ECO:SW] +is_a: ECO:0000109 ! reverse transcription polymerase chain reaction evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000109 ! reverse transcription polymerase chain reaction evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: swolfish +creation_date: 2015-11-03T12:22:46Z + +[Term] +id: ECO:0001809 +name: DNA affinity chromatography evidence +def: "A type of chromatography evidence where immobilized promoter DNA is labeled and bound to a matrix through which a protein mixture is poured and then eluted to purify specific DNA binding proteins." [ECO:SW, PMID:11694305, PMID:11725488] +comment: Biotinylation is commonly used for labeling and beads are commonly used as the matrix. Bound protein will remain attached to the beads. Results can be detected through gel electrophoresis and then sequenced by mass-spectrometry. This technique can be used to demonstrate binding of a purified protein, or to purify the binding protein from crude extract or protein mixture. +synonym: "DNA affinity purification" RELATED [] +is_a: ECO:0000325 ! chromatography evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-03T13:48:49Z + +[Term] +id: ECO:0001810 +name: DNAse footprinting evidence +def: "A type of nucleic acid binding evidence where proteins that have been bound to DNA protect a binding site from enzymatic cleavage with DNAse, thereby detecting protein-DNA interactions." [ECO:SW, PMID:212715, PMID:22194258] +comment: In DNAse footingprinting amplified DNA combined with TF and DNAse is electrophoresed for fragment comparison with a non-TF control sample. TF-bound fragments do not appear in the gel. Such fragments can be isolated, purified, and sequenced. This technique is often used to identify the binding motif of a TF resolving the protected region to 50-100 bp which can be further examined for TF-binding sites. +synonym: "DNase protection" RELATED [] +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000112 "Subsequent DNase I footprinting experiments (Fig. 5d) indicated that His-PhoP protected a single region located from 102 to 47 bp upstream of rovA. This footprint was considered the PhoP site." xsd:string {xref="PMID:21966533"} +property_value: IAO:0000112 "The subsequent DNase I footprinting experiments (Figure 3a) showed that His-OmpR-P protected a single region within the ompR promoter. Therefore, OmpR stimulated its own gene at the transcriptional level, which was mediated through the binding of OmpR-P to its own promoter." xsd:string {xref="PMID:21345178"} +property_value: IAO:0000112 "To precisely determine the PhoP-binding sites of target genes, DNase I footprinting assay was performed on 17 PhoP-dependent promoter DNA regions (both coding and noncoding strands) (Additional file 6). DNase I footprinting results confirmed the direct binding of His-PhoP to these promoter regions in vitro." xsd:string {xref="PMID:18366809"} +property_value: IAO:0000112 "We therefore pursued DNase I footprinting assays on several of the Crp-associated sequences that gave an unusual downshift in our initial EMSA trials, in an effort to identify a consensus binding sequence (see Fig. S2 in the supplemental material). We mapped sites upstream of crp itself, SCO4561 and SCO2977, and identified a consensus binding sequence [GTG(N)6GNCAC]; derivatives of this motif could be found in all of the secondary metabolism-associated target sequences, although notably, one-half of the palindrome seemed to be better conserved than the other [GTG(N)6GNGAN] (Fig. 2C; Table 1)." xsd:string {xref="PMID:23232715"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-03T13:55:15Z + +[Term] +id: ECO:0001811 +name: fluorescence anisotropy evidence +def: "A type of fluorescence evidence based on rapid and quantitative analysis of diverse molecular interactions, enzyme activities, and nucleic acid hybridization which uses a fluorophore to measure the binding constants and kinetics of reactions that cause a change in the rotational time of the molecules." [ECO:SW, PMID:20232898] +comment: The degree of polarization of a fluorophore is inversely related to its molecular rotation. When the fluorophore is bound to a small molecule, the rate at which it tumbles can decrease significantly from when it is bound tightly to a large protein. If the fluorophore is attached to the larger protein in a binding pair, the difference in polarization between bound and unbound states will be smaller and less accurate. The degree of binding is calculated by using the difference in anisotropy of the partially bound, free, and fully bound states measured by titrating the two binding partners. Fluorescence polarixation assays are homogeneous. +synonym: "FA" EXACT [] +synonym: "Fluorescence polarization" RELATED [PMCID:3277431, PMID:20232898] +synonym: "FP" RELATED [PMCID:3277431, PMID:20232898] +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000112 "Since these FA analyses are conducted with 300 nM scOhrR, this suggests that oxidation leads to at least a 100-fold reduction in DNA-binding affinity." xsd:string {xref="PMID:19129220"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-03T14:01:36Z + +[Term] +id: ECO:0001812 +name: genomic systematic evolution of ligands by exponential amplification evidence +def: "A type of systematic evolution of ligands by exponential amplification evidence based on a restricted genomic DNA library to identify naturally occurring genomic aptamers and RNA-protein interaction networks with RNA-binding protein as bait and high-throughput sequencing." [ECO:SW, PMID:20541015, PMID:21720957] +comment: The reported sequences should always be verified for presence in the genome sequence. +synonym: "genomic SELEX" EXACT [PMID:20541015] +is_a: ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-03T14:05:37Z + +[Term] +id: ECO:0001813 +name: heteronuclear single quantum coherence spectroscopy evidence +def: "A type of nuclear magnetic resonance spectroscopy evidence based on two-dimensional NMR for elucidation of the chemical structure of an isolated or synthesized chemical compound where the characteristic transfer magnetization of a proton to a nitrogen or carbon isotope is monitored by NMR, generating a specific peak in the spectrum." [ECO:SW, PMID:19856946] +comment: Chemical shifts in the spectrum indicating binding can be detected from analysis of a protein spectrum in the presence or absence of its cognate DNA binding site. +synonym: "HSQC" EXACT [PMID:19856946] +is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-03T14:11:06Z + +[Term] +id: ECO:0001814 +name: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence +def: "A type of nucleic acid binding evidence where the synthetic molecule methidiumpropyl-EDTA (MPE) is used to cleave ligand-protected DNA, followed by analysis of the restriction fragments to generate a footprint (i.e. size and location) of small molecule binding sites on the DNA." [ECO:SW, PMID:6225070] +synonym: "Methidiumpropyl-EDTA Fe(II) footprinting" RELATED [PMID:6225070] +synonym: "MPE Fe(II) footprinting" RELATED [PMID:6225070] +synonym: "MPE-EDTA Fe(II) footprinting" RELATED [PMID:6225070] +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-03T14:24:04Z + +[Term] +id: ECO:0001815 +name: copper-phenanthroline footprinting evidence +def: "A type of nucleic acid binding evidence where nucleic acid that has been bound to protein is cleaved with 1,10-phenanthroline-copper complex resulting in a high-resolution footprint of sequence-specific protein-DNA contacts." [ECO:SW, PMID:11691942] +synonym: "1,10-Phenanthroline-copper footprinting" EXACT [PMID:1384472] +synonym: "OP-Cu Complex" RELATED [PMID:1384472] +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T08:37:51Z + +[Term] +id: ECO:0001816 +name: green fluorescent protein reporter gene assay evidence +alt_id: ECO:0000296 +def: "A type of reporter gene assay evidence based on the fusion of select genes with the green fluorescent protein (GFP) gene for detection with bioluminescence of the gene product when exposed to blue ultraviolet light." [ECO:SW, PMID:11989662] +synonym: "GFP promoter fusion" RELATED [] +synonym: "GFP reporter gene assay" EXACT [PMID:11989662] +synonym: "green fluorescent protein promoter fusion" RELATED [] +synonym: "green fluorescent protein transcript localization evidence" EXACT [] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T08:58:42Z + +[Term] +id: ECO:0001817 +name: glutathione S-transferase pull-down assay evidence +def: "A type of bait-prey hybrid interaction evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions." [ECO:SW, PMID:26096507] +comment: The recombinant protein can also be eluted following the pull-down and analyzed further with qPCR or sequencing. +synonym: "GST pull-down assay" EXACT [PMID:26096507] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T09:05:56Z + +[Term] +id: ECO:0001818 +name: hydroxyl-radical footprinting evidence +def: "A type of nucleic acid binding evidence used to identify protein-binding sites on the DNA molecule where DNA that has been bound to protein is digested with hydroxyl radical produced by reduction of hydrogen peroxide with iron (II), followed by separating the cleavage products on a denaturing electrophoresis gel." [ECO:SW, PMID:18546600, PMID:19378159, PMID:3090544] +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T09:20:26Z + +[Term] +id: ECO:0001819 +name: primer extension assay evidence +def: "A type of transcript expression evidence used to determine expression levels of mRNA where a labeled synthetic oligonucleotide primer is annealed to mRNA downstream of the presumed transcription start site of a gene. The mRNA is extended with reverse transcriptase, producing cDNA which is electrophoresed, and the band size is enables determination of the 5' end of the mRNA (i.e. the transcription start site)." [ECO:SW, PMID:23378648] +comment: The most commonly used radiolabel is 32P. +is_a: ECO:0000009 ! transcript expression evidence +property_value: IAO:0000112 "The primer extension assay (Fig. 6a) defined the transcription start sites the three sRNA genes qrr2 - 4, and this assay also indicated that the promoter activity of all the thee qrr genes was under the positive control of OpaR." xsd:string {xref="PMID:22506036"} +property_value: IAO:0000112 "The primer extension assay detected two transcriptional start sites located at 343 and 78 bp upstream of rovA (Fig. 2); therefore, two promoters (named P2 and P1, respectively) were transcribed for rovA." xsd:string {xref="PMID:21966533"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T09:33:18Z + +[Term] +id: ECO:0001820 +name: rapid amplification of cDNA ends polymerase chain reaction evidence +def: "A type of nucleotide sequencing assay evidence in which RT-PCR (cDNA synthesis) is first used to produce a cDNA copy of a region of the RNA transcript being investigated followed by PCR to capture either the unknown 5' or 3' end of the transcript for sequencing, depending on whether 5' RACE-PCR or 3' RACE-PCR are being undertaken." [ECO:SW, PMID:17498297, PMID:7685466] +comment: 5' and 3' RACE-PCR utilize different protocols to amplify an unknown end using the known sequence of the center of the transcript. For cDNA synthesis, 5' RACE-PCR uses an anti-sense oligonucleotide primer (gene specific primer (GSP)) that recognizes a known sequence in the middle of the gene of interest and the reverse transcriptase to add base pairs to the 3' end of the primer. Next, terminal deoxynucleotidyl transferase (TdT) is used to add a string of identical nucleotides to the 3' end of the cDNA. A PCR reaction is then carried out, using a second anti-sense gene specific primer (GSP2) that binds to the known sequence, and a sense (forward) universal primer (UP) that binds the homopolymeric tail added to the 3' ends of the cDNAs to amplify a cDNA product from the 5' end. In contrast, 3' RACE-PCR utilizes the naturally occurring 3' polyA tail of the transcript and uses an Oligo-dT-adaptor primer (a primer with a short sequence of deoxy-thymine nucleotides) that complements the polyA tail and adds a special adaptor sequence to the 5' end of each cDNA. PCR is then used to amplify 3' cDNA from a known region using a sense GSP, and an anti-sense primer complementary to the adaptor sequence. +comment: RACE PCR is frequently used to verify transcription start sites relevant to the function of transcription factor binding sites such as repression. +synonym: "3’ RACE-PCR" EXACT [] +synonym: "5’ RACE-PCR" EXACT [] +synonym: "RACE PCR" EXACT [PMI:17498297] +is_a: ECO:0000219 ! nucleotide sequencing assay evidence +property_value: IAO:0000112 "5' RACE analysis was employed to localize the espR promoter using RNA extracted from Mtb H37Rv grown to mid-log phase. The espR transcript starts with a poly-G (7) sequence 144 bp upstream of the translational start codon." xsd:string {xref="PMID:22479184"} +property_value: IAO:0000112 "The 5'-end of the espA mRNA was located 66 bp upstream of the translation start codon using 5' RACE (Fig. S3)." xsd:string {xref="PMID:22479184"} +property_value: IAO:0000112 "Using 5' rapid amplification of cDNA ends (RACE), we mapped the transcriptional start sites of rpoQ and VF_A1016 (Fig. 4A). Neither start site was detected in the DeltarpoQ mutant, supporting the idea that transcription of rpoQ and VF_A1016 requires RpoQ (data not shown)." xsd:string {xref="PMID:22233679"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T09:48:38Z + +[Term] +id: ECO:0001821 +name: RNA sequencing assay evidence +comment: Use ECO:0000295 (RNA-seq evidence) in place of this term. +property_value: IAO:0000234 "CollecTF" xsd:string +is_obsolete: true +created_by: swolfish +creation_date: 2015-11-04T10:15:57Z + +[Term] +id: ECO:0001822 +name: survival rate analysis evidence +def: "A type of knockout evidence based on the survival of an organism in a particular environment where a gene for an enzyme or regulator is knocked out and results are used as a natural reporter." [ECO:SW] +is_a: ECO:0001091 ! knockout phenotypic evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T10:48:59Z + +[Term] +id: ECO:0001823 +name: x-ray crystallography evidence +def: "A type of crystallography evidence where a purified sample at high concentration is crystallised and the crystals are exposed to an x ray beam to obtain three dimensional molecular structure of proteins and biological macromolecules." [ECO:SW, PMID:23135450, PMID:24648090] +comment: X-ray crystallography can be used for a crystal used to probe at the tridimensional structure of proteins. In some cases, it is possible to co-crystallize the protein bound to its DNA binding site, providing detail on the particular arrangement of the two components. +is_a: ECO:0001034 ! crystallography evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-04T10:56:22Z + +[Term] +id: ECO:0001824 +name: DNA adenine methyltransferase identification evidence +def: "A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site." [ECO:SW, PMID:16938559, PMID:17545983, PMID:19588092] +comment: The methylation tag is detected with methylation-sensitive restriction enzymes, DpnI and DpnII. +synonym: "DamID" EXACT [PMID:19588092] +is_a: ECO:0000023 ! affinity evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-05T15:56:13Z + +[Term] +id: ECO:0001825 +name: isothermal titration calorimetry evidence +def: "A type of affinity evidence where the absorbed or released heat of a biomolecular binding event is directly measured in a reference cell and sample cell after the addition of a ligand using a microcalorimeter for a complete thermodynamic profile of the molecular interaction." [ECO:SW, PMID:10527727] +comment: When ITC is used to study TF, the temperature of two identical cells containing a known concentration of TF is monitored. After the ligand is added to the cells in precisely measured aliquots, the temperature difference in each cell is observed to determine target binding. This value can be used to compute the energetics of the reaction and hence, binding affinity of the TF for the DNA fragment. The thermodynamic profile that is measured includes the values of binding constant (K(a)), stoichiometry (n), and the enthalpy of binding (DeltaH(b)). +synonym: "ITC" EXACT [PMID:10527727] +is_a: ECO:0000023 ! affinity evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-05T16:06:20Z + +[Term] +id: ECO:0001826 +name: ultraviolet light footprinting evidence +def: "A type of nucleic acid binding evidence where DNA is non-specifically fragmented with ultraviolet light while protein-bound regions are protected from UV damage and strand breakage patterns are analyzed by PAGE and sequenced to detect protein-DNA contacts." [ECO:SW, PMID:2842760, PMID:7602584] +synonym: "ultraviolet footprinting" RELATED [] +synonym: "UV footprinting" RELATED [] +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-05T16:13:36Z + +[Term] +id: ECO:0001827 +name: methylation interference footprinting evidence +def: "A type of nucleic acid binding evidence used to identify protein binding sites on the DNA molecule where multiple copies of a DNA fragment containing a putative TF-binding site are randomly methylated with dimethyl sulfate (DMS) and cleaved at the methyl group, followed by separating the cleavage products on a denaturing electrophoresis gel." [ECO:SW, PMID:1583685] +synonym: "methylation interference footprinting evidence" EXACT [] +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-05T16:16:27Z + +[Term] +id: ECO:0001828 +name: inference of sequence features from visual inspection used in manual assertion +def: "A type of inference of sequence features from visual inspection that is used in a manual assertion." [ECO:RCJ] +synonym: "visual sequence inspection evidence" BROAD [] +is_a: ECO:0007751 {is_inferred="true"} ! inferential evidence used in manual assertion +is_a: ECO:0007850 {is_inferred="true"} ! inference of sequence features from visual inspection +intersection_of: ECO:0007850 ! inference of sequence features from visual inspection +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: swolfish +creation_date: 2015-11-10T14:40:16Z + +[Term] +id: ECO:0001829 +name: ferric uptake regulator titration assay evidence +def: "A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis." [ECO:SW, PMID:10713425, PMID:7642488, PMID:8107138] +synonym: "Fluorescence polarization" RELATED [PMID:15689115] +synonym: "FURTA" EXACT [PMID:10713425] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: swolfish +creation_date: 2015-11-10T14:55:36Z + +[Term] +id: ECO:0001830 +name: host colonization assay evidence +def: "A type of experimental evidence in which the colonization or adhesion capacity is measured." [] +comment: Has to do with the ability of the bacterium or virus to bind to a host. +synonym: "adhesion assay evidence" EXACT [] +is_a: ECO:0000006 ! experimental evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001831 +name: host colonization assay evidence used in automatic assertion +def: "A type of host colonization assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001830 ! host colonization assay evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0001830 ! host colonization assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001832 +name: host colonization assay evidence used in manual assertion +def: "A type of host colonization assay evidence that is used in a manual assertion." [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001830 ! host colonization assay evidence +intersection_of: ECO:0001830 ! host colonization assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001833 +name: infection assay evidence +def: "A type of host colonization assay evidence in which the infection capacity is measured by assessing changes in the host." [] +comment: Has to do with the outcome of a bacterial or viral / host interaction, be it a measure of cell death, cytotoxicity, invasion, etc. +is_a: ECO:0001830 ! host colonization assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001834 +name: infection assay evidence used in automatic assertion +def: "A type of infection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001831 {is_inferred="true"} ! host colonization assay evidence used in automatic assertion +is_a: ECO:0001833 ! infection assay evidence +intersection_of: ECO:0001833 ! infection assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001835 +name: infection assay evidence used in manual assertion +def: "A type of infection assay evidence that is used in a manual assertion." [] +is_a: ECO:0001832 {is_inferred="true"} ! host colonization assay evidence used in manual assertion +is_a: ECO:0001833 ! infection assay evidence +intersection_of: ECO:0001833 ! infection assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: jbmunro +creation_date: 2019-05-06T12:00:00Z + +[Term] +id: ECO:0001836 +name: in situ hybridization evidence +def: "A type of expression pattern evidence in which single-stranded DNA or RNA (probe) are annealed to complementary DNA or RNA in a portion or section of tissue." [] +is_a: ECO:0000008 ! expression pattern evidence +property_value: IAO:0000234 "planarian anatomy ontology (PLANA)" xsd:string +created_by: jbmunro +creation_date: 2019-09-26T12:00:00Z + +[Term] +id: ECO:0001837 +name: in situ hybridization evidence used in automatic assertion +def: "A type of in situ hybridization evidence that is used in an automatic assertion." [] +is_a: ECO:0001836 ! in situ hybridization evidence +is_a: ECO:0007298 {is_inferred="true"} ! expression pattern evidence used in automatic assertion +intersection_of: ECO:0001836 ! in situ hybridization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: jbmunro +creation_date: 2019-09-26T12:00:00Z + +[Term] +id: ECO:0001838 +name: in situ hybridization evidence used in manual assertion +def: "A type of in situ hybridization evidence that is used in a manual assertion." [] +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0001836 ! in situ hybridization evidence +intersection_of: ECO:0001836 ! in situ hybridization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: jbmunro +creation_date: 2019-09-26T12:00:00Z + +[Term] +id: ECO:0001839 +name: colorimetric in situ hybridization evidence +def: "A type of nucleic acid localization evidence and in situ hybridization evidence resulting from the use of colorimetric probes to detect complementary sequences of nucleic acids." [PMID:23497040] +is_a: ECO:0001836 ! in situ hybridization evidence +property_value: IAO:0000234 "planarian anatomy ontology (PLANA)" xsd:string +created_by: jbmunro +creation_date: 2019-09-26T12:00:00Z + +[Term] +id: ECO:0001840 +name: colorimetric in situ hybridization evidence used in automatic assertion +def: "A type of colorimetric in situ hybridization evidence that is used in an automatic assertion." [] +is_a: ECO:0001837 {is_inferred="true"} ! in situ hybridization evidence used in automatic assertion +is_a: ECO:0001839 ! colorimetric in situ hybridization evidence +intersection_of: ECO:0001839 ! colorimetric in situ hybridization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: jbmunro +creation_date: 2019-09-26T12:00:00Z + +[Term] +id: ECO:0001841 +name: colorimetric in situ hybridization evidence used in manual assertion +def: "A type of colorimetric in situ hybridization evidence that is used in a manual assertion." [] +is_a: ECO:0001838 {is_inferred="true"} ! in situ hybridization evidence used in manual assertion +is_a: ECO:0001839 ! colorimetric in situ hybridization evidence +intersection_of: ECO:0001839 ! colorimetric in situ hybridization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: jbmunro +creation_date: 2019-09-26T12:00:00Z + +[Term] +id: ECO:0001842 +name: random mutagenesis of specific target DNA evidence +def: "A type of random mutagenesis phenotypic evidence resulting from the random mutation of a specifically targeted region of DNA." [] +is_a: ECO:0005529 ! random mutagenesis phenotypic evidence +property_value: IAO:0000234 "ECO" xsd:string +created_by: jbmunro +creation_date: 2019-12-09T12:00:00Z + +[Term] +id: ECO:0001843 +name: random mutagenesis of specific target DNA evidence used in automatic assertion +def: "A type of random mutagenesis of specific target DNA evidence that is used in an automatic assertion." [] +is_a: ECO:0001842 ! random mutagenesis of specific target DNA evidence +is_a: ECO:0007478 {is_inferred="true"} ! random mutagenesis evidence used in automatic assertion +intersection_of: ECO:0001842 ! random mutagenesis of specific target DNA evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 "ECO" xsd:string +created_by: jbmunro +creation_date: 2019-12-09T12:00:00Z + +[Term] +id: ECO:0001844 +name: random mutagenesis of specific target DNA evidence used in manual assertion +def: "A type of random mutagenesis of specific target DNA evidence that is used in a manual assertion." [] +is_a: ECO:0001842 ! random mutagenesis of specific target DNA evidence +is_a: ECO:0005530 {is_inferred="true"} ! random mutagenesis evidence used in manual assertion +intersection_of: ECO:0001842 ! random mutagenesis of specific target DNA evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 "ECO" xsd:string +created_by: jbmunro +creation_date: 2019-12-09T12:00:00Z + +[Term] +id: ECO:0001845 +name: cell population optical density evidence +def: "A type of cell-based assay evidence in which optical density is used to measure some aspect of a population of cells. Examples of aspects that can affect optical density measurements include, but are not limited to, cell number and cell shape." [] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 "CACAO" xsd:string +created_by: jbmunro +creation_date: 2020-01-28T12:00:00Z + +[Term] +id: ECO:0001846 +name: cell population optical density evidence used in automatic assertion +def: "A type of cell population optical density evidence that is used in an automatic assertion." [] +is_a: ECO:0001845 ! cell population optical density evidence +property_value: IAO:0000234 "CACAO" xsd:string +created_by: jbmunro +creation_date: 2020-01-28T12:00:00Z + +[Term] +id: ECO:0001847 +name: cell population optical density evidence used in manual assertion +def: "A type of cell population optical density evidence that is used in a manual assertion." [] +is_a: ECO:0001845 ! cell population optical density evidence +property_value: IAO:0000234 "CACAO" xsd:string +created_by: jbmunro +creation_date: 2020-01-28T12:00:00Z + +[Term] +id: ECO:0005004 +name: cell viability assay evidence +def: "A type of cell-based assay evidence resulting from analyzing the ability of cells to survive or live successfully." [NBK:144065] +is_a: ECO:0001565 ! cell-based assay evidence +created_by: snadendla +creation_date: 2015-02-13T12:08:01Z + +[Term] +id: ECO:0005007 +name: cell proliferation assay evidence +def: "A type of cell-based assay evidence resulting from the measurement of the number of cells, which is a reflection of the balance between cell division and cell loss through cell differentiation or cell death." [ECO:RCT] {comment="PMID:11057898", comment="GO:0008283"} +is_a: ECO:0001565 ! cell-based assay evidence +created_by: snadendla +creation_date: 2015-02-11T16:27:13Z + +[Term] +id: ECO:0005008 +name: DNA synthesis cell proliferation assay evidence +def: "A type of cell proliferation assay evidence resulting from the measure of DNA synthesis in the presece of a label." [PMC:3908118] +is_a: ECO:0005007 ! cell proliferation assay evidence +created_by: snadendla +creation_date: 2015-02-11T16:30:10Z + +[Term] +id: ECO:0005011 +name: ATP bioluminescence assay evidence +def: "A type of cell viability assay evidence resulting from the analysis of ATP concentration (by measuring light intensity) when luciferase catalyzes the oxidation of luciferin in the presence of ATP, magnesium ions and molecular oxygen." [PMID:15344559] +is_a: ECO:0005004 ! cell viability assay evidence +created_by: snadendla +creation_date: 2015-02-11T16:47:30Z + +[Term] +id: ECO:0005012 +name: cytotoxicity assay evidence +def: "A type of cell-based assay evidence resulting from the ability of an agent to induce cellular necrosis or apoptosis and the subsequent analysis of cell death mechanism." [] +is_a: ECO:0001565 ! cell-based assay evidence +created_by: snadendla +creation_date: 2015-02-12T11:39:35Z + +[Term] +id: ECO:0005014 +name: in vitro cell based assay evidence +comment: Use children of ECO:0001565 (cell-based assay evidence) in place of this term. +is_obsolete: true +created_by: snadendla +creation_date: 2015-02-12T13:26:55Z + +[Term] +id: ECO:0005019 +name: staining evidence +def: "A type of direct assay evidence resulting from the use of stains or dyes to aid in analysis of a microscopic image." [ECO:RCT] +is_a: ECO:0000002 ! direct assay evidence +created_by: snadendla +creation_date: 2015-02-19T11:48:57Z + +[Term] +id: ECO:0005021 +name: chemotaxis assay evidence +def: "A type of cell-based assay evidence resulting from the evaluation of chemotactic ability (i.e. the movement, or lack of movement, in response to a chemical stimulus) of prokaryotic or eukaryotic cells." [PMC:3667641] +is_a: ECO:0001565 ! cell-based assay evidence +created_by: snadendla +creation_date: 2015-02-19T13:10:20Z + +[Term] +id: ECO:0005027 +name: genetic transformation evidence +def: "A type of mutant phenotype evidence resulting from the conversion of one genotype into another by the introduction of exogenous DNA." [ECO:RCT] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: snadendla +creation_date: 2015-03-16T15:33:50Z + +[Term] +id: ECO:0005031 +name: structure determination evidence +def: "A type of experimental evidence resulting from the visualization and examination of chemical and/or molecular structures." [ECO:RCT] +is_a: ECO:0000006 ! experimental evidence +property_value: IAO:0000112 "The secondary structure of VapC10 (Figure S2), predicted with the 3DJIGSAW prediction tool [36] and the DALI server [37], exhibited homology with several well studied VapC toxins, such as the first PIN domain structure for the protein PAE2754 from the archae bacterium Pyrobaculum aerophilum (30)." xsd:string {xref="PMID:24260461"} +created_by: snadendla +creation_date: 2015-03-20T12:16:17Z + +[Term] +id: ECO:0005033 +name: electron microscopy evidence +def: "A type of microscopy evidence and structure determination evidence resulting from an electron beam utilized to create an image." [ECO:RCT] +is_a: ECO:0001098 ! microscopy evidence +is_a: ECO:0005031 ! structure determination evidence +property_value: IAO:0000112 "Electron micrographs showed that all the cells are rod-shaped and those of the parent strain Ye9 and the envZ mutant strain EZ10 are flagellated, but the ompR mutant cells lack flagella." xsd:string {xref="PMID:20830609"} +created_by: snadendla +creation_date: 2015-03-20T12:21:48Z + +[Term] +id: ECO:0005034 +name: apoptotic assay evidence +def: "A type of cell-based assay evidence resulting from the occurrence, visualization, and/or analysis of apoptosis." [ECO:RCT] +is_a: ECO:0001565 ! cell-based assay evidence +created_by: snadendla +creation_date: 2015-04-07T21:58:24Z + +[Term] +id: ECO:0005500 +name: in vivo polyADP-ribosylation assay evidence +def: "A type of polyADP-ribosylation assay evidence used in an in vivo experiment." [ECO:SN, PMID:2820766, SIB:PG] +is_a: ECO:0001557 ! polyADP-ribosylation assay evidence +created_by: snadendla +creation_date: 2015-06-15T15:35:05Z + +[Term] +id: ECO:0005501 +name: in vivo polyADP-ribosylation assay evidence used in manual assertion +def: "A type of in vivo polyADP-ribosylation assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005500 ! in vivo polyADP-ribosylation assay evidence +intersection_of: ECO:0005500 ! in vivo polyADP-ribosylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2015-06-15T15:37:23Z + +[Term] +id: ECO:0005502 +name: ex vivo assay evidence +def: "A type of direct assay evidence derived by studying an organ, tissues, or cells taken from an organism and studied in an external environment with the minimum alteration of natural conditions." [ECO:SN, PMID:16305312, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf] +is_a: ECO:0000002 ! direct assay evidence +created_by: snadendla +creation_date: 2015-06-26T11:46:15Z + +[Term] +id: ECO:0005503 +name: two-dimensional polyacrylamide gel electrophoresis evidence +def: "A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape." [ECO:SW, url:http\://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html] +comment: Two-dimensional polyacrylamide gel electrophoresis is commonly employed to establish a proteome for an organism and demonstrate expression changes. +synonym: "2D PAGE" EXACT [] +synonym: "2D-PAGE" EXACT [] +is_a: ECO:0000337 ! gel electrophoresis evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-01T16:23:20Z + +[Term] +id: ECO:0005504 +name: spectrometry evidence +def: "A type of experimental evidence resulting from the use of a spectrometer." [ECO:RCT] +is_a: ECO:0000006 ! experimental evidence +created_by: snadendla +creation_date: 2015-08-14T15:48:08Z + +[Term] +id: ECO:0005505 +name: regular expression motif search evidence +def: "A type of motif similarity evidence where the motif, a local alignment of sequences, is represented by a regular expression, which captures the variability of nucleotides at each position in a discrete form and can then be used to search genetic sequences by identifying matches to the regular expression." [ECO:SW, PMID:17337630] +comment: Regular expression is a computational tool used for determining whether a given genome contains a DNA region resembling a transcription factor binding motif. For example A/T=W indicates A or T may be present in a column of the alignment. (PMID: 20708667). +is_a: ECO:0000028 ! motif similarity evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-09T13:51:52Z + +[Term] +id: ECO:0005506 +name: missense mutation phenotypic evidence +def: "A type of point mutation phenotypic evidence resulting from a change in a base (or bases) causing the substitution of one amino acid for another." [SO:0001583] +synonym: "missense mutation evidence" EXACT [] +is_a: ECO:0001113 ! point mutation phenotypic evidence +created_by: snadendla +creation_date: 2015-09-18T16:52:34Z + +[Term] +id: ECO:0005507 +name: nonsense mutation phenotypic evidence +def: "A type of point mutation phenotypic evidence resulting in an incomplete and usually nonfunctional protein product because of a premature stop codon." [url:https\://ghr.nlm.nih.gov/primer/mutationsanddisorders/possiblemutations] +synonym: "nonsense mutation evidence" EXACT [] +is_a: ECO:0001113 ! point mutation phenotypic evidence +created_by: snadendla +creation_date: 2015-09-18T16:55:32Z + +[Term] +id: ECO:0005508 +name: silent mutation evidence +def: "A type of point mutation phenotypic evidence resulting from the change of a DNA nucleotide that does not cause any changes in the amino acid sequence." [url:http\://www.sciencemag.org/news/2006/12/sound-silent-mutation] +is_a: ECO:0001113 ! point mutation phenotypic evidence +created_by: snadendla +creation_date: 2015-09-18T17:01:12Z + +[Term] +id: ECO:0005509 +name: insertion mutation phenotypic evidence +def: "A type of mutant phenotype evidence resulting from a mutation in which there is an insertion of one or more contiguous nucleotides." [GO:0070705] +synonym: "insertion mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: snadendla +creation_date: 2015-09-18T17:02:03Z + +[Term] +id: ECO:0005511 +name: duplication mutation evidence +def: "A type of experimental phenotypic evidence resulting from a mutation in which inserted nucleotide(s) have a sequence identical to, or derived from, adjacent nucleotides." [SO:1000035] +is_a: ECO:0000059 ! experimental phenotypic evidence +created_by: snadendla +creation_date: 2015-09-18T17:03:25Z + +[Term] +id: ECO:0005512 +name: frameshift mutation phenotypic evidence +def: "A type of mutant phenotype evidence resulting from any mutation that causes an insertion or deletion of nucleotides in a DNA sequence that is not divisible by three and therefore changes the codon reading frame and the translated amino acids." [url:https\://ghr.nlm.nih.gov/primer/mutationsanddisorders/possiblemutations] +synonym: "frameshift mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: snadendla +creation_date: 2015-09-18T17:05:49Z + +[Term] +id: ECO:0005513 +name: repeat expansion mutation phenotypic evidence +def: "A type of mutant phenotype evidence resulting from a mutation that increases the number of times that a DNA sequence is repeated." [PMID:9397685, url:https\://ghr.nlm.nih.gov/primer/mutationsanddisorders/possiblemutations] +synonym: "repeat expansion mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: snadendla +creation_date: 2015-09-18T17:06:34Z + +[Term] +id: ECO:0005514 +name: splice site mutation phenotypic evidence +def: "A type of mutant phenotype evidence resulting from a mutation in which there is a change in nucleotides at a splice site." [ECO:RCT] +synonym: "splice site mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: snadendla +creation_date: 2015-09-18T17:07:29Z + +[Term] +id: ECO:0005515 +name: translocation mutation phenotypic evidence +def: "A type of mutant phenotype evidence resulting from a mutation in which a region of a chromosome is transferred to a nonhomologous chromosome." [ECO:RCT] +synonym: "translocation mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +created_by: snadendla +creation_date: 2015-09-18T17:08:01Z + +[Term] +id: ECO:0005516 +name: molecule detection assay evidence +def: "A type of experimental evidence resulting from the detection and analysis of molecular markers." [ECO:RCT] +is_a: ECO:0000006 ! experimental evidence +created_by: snadendla +creation_date: 2015-09-24T15:54:21Z + +[Term] +id: ECO:0005517 +name: protein detection assay evidence +def: "A type of molecule detection assay evidence resulting from the detection and quantification of a protein, or multiple proteins." [ECO:RCT] +is_a: ECO:0005516 ! molecule detection assay evidence +created_by: snadendla +creation_date: 2015-09-25T14:27:40Z + +[Term] +id: ECO:0005518 +name: RNA detection assay evidence +def: "A type of molecule detection assay evidence resulting from the detection of specific RNAs." [ECO:RCT] +is_a: ECO:0005516 ! molecule detection assay evidence +created_by: snadendla +creation_date: 2015-09-25T14:43:53Z + +[Term] +id: ECO:0005519 +name: DNA detection assay evidence +def: "A type of molecule detection assay evidence resulting from the detection and visualization of a sequence of DNA." [ECO:RCT] +is_a: ECO:0005516 ! molecule detection assay evidence +created_by: snadendla +creation_date: 2015-09-25T19:33:25Z + +[Term] +id: ECO:0005520 +name: interferometric reflectance imaging sensor evidence +def: "A type of molecule detection assay evidence based on changes in surface-reflected light due to ligand binding on the surface of a semi-transparent substrate to detect proteins, DNA, and other biological material in a multiplexed, high-throughput microarray format without labels." [ECO:SW, PMID:21587155, PMID:24271115] +comment: Differences in the optical path lengths between a surface layer and a buried silica layer can be measured very precisely allowing optical height information to be converted to accumulated mass on the surface. For the purpose of TF-binding site identification, IRIS is coupled with DNA-arrays with immobilized potential binding regions exposed to the protein of interest and eluted. Protein-bound fragments exhibit changes in reflectance that can be used to gauge binding quantitatively. +synonym: "IRIS" EXACT [] +synonym: "Spectral Reflectance Imaging Biosensor" EXACT [] +synonym: "SRIB" EXACT [] +is_a: ECO:0005516 ! molecule detection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-28T16:01:01Z + +[Term] +id: ECO:0005521 +name: S1 nuclease protection assay evidence +def: "A type of nuclease protection assay evidence where RNA is hybridized with complementary DNA probes and exposed to S1 nuclease for unbound RNA degradation after which the intact RNA is run on a gel for probe size determination and RNA identification to detect and map specific RNAs in a complex mixture of total cellular RNA." [ECO:SW, PMID:21390683] +is_a: ECO:0001106 ! nuclease protection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-28T17:16:56Z + +[Term] +id: ECO:0005522 +name: DNA dot blot assay evidence +def: "A type of DNA detection assay evidence resulting from evaluation of the relative abundance of target sequences hybridizing to DNA samples that are immobilized on a nitrocellulose or nylon membrane." [PMID:18265189] +is_a: ECO:0005519 ! DNA detection assay evidence +created_by: snadendla +creation_date: 2015-09-30T10:03:03Z + +[Term] +id: ECO:0005523 +name: DNA dot blot assay evidence used in manual assertion +def: "A type of DNA dot blot assay evidence that is used in a manual assertion." [ECO:SN, ECO:SW] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0005522 ! DNA dot blot assay evidence +intersection_of: ECO:0005522 ! DNA dot blot assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2015-09-30T10:04:00Z + +[Term] +id: ECO:0005526 +name: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence +def: "A type of protein detection assay evidence where soft ionization is used for the analysis of biomolecules for mass determination in a three step process of plate preparation, radiation, and ionization with high throughput technology." [ECO:SW, PMID:21964792, PMID:25354905] +comment: MALDI-TOF MS is commonly used for protein identification in constructing a proteome where protein fingerprints obtained by microbial cells are compared with a database of reference spectra by means of various algorithms integrated in systems recently made commercially available. +synonym: "MALDI-TOF Mass Spectrometry" EXACT [] +synonym: "MALDI-TOF-MS" EXACT [] +is_a: ECO:0005517 ! protein detection assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-30T10:51:56Z + +[Term] +id: ECO:0005527 +name: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion +def: "A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in a manual assertion." [ECO:SN, ECO:SW] +synonym: "MALDI-TOF mass spectrometry evidence" BROAD [] +synonym: "MALDI-TOF-MS evidence" BROAD [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence +intersection_of: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2015-09-30T10:58:51Z + +[Term] +id: ECO:0005528 +name: site-directed mutagenesis phenotypic evidence +def: "A type of mutant phenotype phenotype evidence based on target-specified mutagenesis for the characterization of various gene and protein interactions, structures, and functions where oligonucleotide primers are used to alter a nucleotide sequence." [ECO:SW, PMID:21204030, PMID:24011050, PMID:3541892] +comment: Site-directed mutagenesis is often performed in tandem with EMSA. It is often implemented only in conserved motif positions or serially through all positions of a site. +synonym: "site directed mutagenesis" EXACT [] +synonym: "site-directed mutagenesis evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000112 "If the AGAA sequence is mutated CTCC (Pxyn2aM, see Table 1), no DNA-protein complex is formed under either repressing or inducing conditions (Figure 1). This result indicates that this part of the xyn2 promoter is essential for binding a transcription factor under repressing conditions." xsd:string {xref="PMID:21087492"} +property_value: IAO:0000112 "To identify key residues required for specific GlnR binding we mutated the highly conserved AC-n9-AC and AT-n9-AC DNA binding motifs. Figure 4 shows that the highly conserved adenosine residues in the motif are critical as GlnR binding is abolished when these residues are mutated." xsd:string {xref="PMID:23642041"} +property_value: IAO:0000112 "We also tested a hyaS promoter in which one (highest score) of the three putative AdpA-binding sites was mutated (at position -134 to -129, see Additional file 3: Figure S1a): the affinity of AdpA for this promoter region was reduced and one protein-DNA complex disappeared (Additional file 3: Figure S1b). These results suggest that one dimer of AdpA binds the adjacent sites -129 and -123 of S. lividans hyaS promoter and another dimer binds the -100 site resulting in the formation of the two DNA-AdpA complexes depicted in Figure 2." xsd:string {xref="PMID:24694298"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-30T11:34:41Z + +[Term] +id: ECO:0005529 +name: random mutagenesis phenotypic evidence +def: "A type of mutant phenotype evidence where a mutagenic product or process is used to alter the nucleotide sequence and thus induce genetic mutation. Examples include, but are not limited to mutagenic compounds, irradiation, PCR w/ degenerate primers, etc." [ECO:SW, PMID:15153637, PMID:18265275] +comment: Random mutagenesis is not often used in TF-binding site determination; however, it is sometimes used to investigate the effect of mutations in TF binding domains in conjunction with expression assays. Random mutations can also be introduced via chemicals, error-prone PCR (EP-PCR), degenerate oligonucleotides-Pfu(DOP), UV irradiation, mutator strains, nucleotide analogs, or DNA recombination. +synonym: "random mutagenesis evidence" NARROW [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-30T11:55:03Z + +[Term] +id: ECO:0005530 +name: random mutagenesis evidence used in manual assertion +def: "A type of random mutagenesis phenotypic evidence that is used in a manual assertion." [] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005529 ! random mutagenesis phenotypic evidence +intersection_of: ECO:0005529 ! random mutagenesis phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: snadendla +creation_date: 2015-09-30T11:57:00Z + +[Term] +id: ECO:0005531 +name: motif discovery evidence +def: "A type of multiple sequence alignment evidence based on a set of algorithms that infer over-represented motifs from a set of biological sequences returning one or more local multiple sequence alignment defining the inferred motifs." [ECO:SW, PMID:10812473, PMID:16845028, PMID:8211139] +comment: Relationship type between sequences (e.g. orthologs from multiple genomes or co-expressed genes in the same genome) is not necessarily assumed. The local multiple sequence alignment is typically ungapped. A common application of motif discovery is to infer the binding motif of a given transcription factor (TF). This can be based on the results of a whole-genome expression analysis, assuming that co-expressed genes are co-regulated by the same TF, from peaks in ChIP-seq experiments with the TF, or from the comparative genomics analysis of the promoter regions of orthologs. {xref="PMID:19892760", xref="PMID:23032607"} +is_a: ECO:0005555 ! multiple sequence alignment evidence +property_value: IAO:0000112 "A sequence alignment of the phlA gene promoter region from strain 2P24 with other well-studied 2,4-DAPG producing Pseudomonas strains revealed a very well conserved palindromic sequence GAAACN5GTTTC (Fig. S1)." xsd:string {xref="PMID:23209661"} +property_value: IAO:0000112 "In order to identify a potential regulatory motif for KstR2, we used the promoter regions of the kstR2 orthologues as a training set for the motif identification program MEME (Bailey & Elkan, 1994). This identified a potential regulatory sequence that contains a 14 bp inverted palindromic motif AnCAAGnnCTTGnT (Fig. 2)." xsd:string {xref="PMID:20167624"} +property_value: IAO:0000112 "We mapped sites upstream of crp itself, SCO4561 and SCO2977, and identified a consensus binding sequence [GTG(N)6GNCAC]; derivatives of this motif could be found in all of the secondary metabolism-associated target sequences, although notably, one-half of the palindrome seemed to be better conserved than the other [GTG(N)6GNGAN] (Fig. 2C; Table 1)." xsd:string {xref="PMID:23232715"} +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-30T15:04:45Z + +[Term] +id: ECO:0005532 +name: consensus search evidence +def: "A type of motif similarity evidence where the motif is represented by a consensus sequence which incorporates the most frequent nucleotide at each position acting as a generic representative for the sequences in the alignment, and is compared to the genetic sequence for a perfect or imperfect match." [ECO:SW, PMID:15130839] +comment: This technique is used to identify transcription factor binding sites by searching a bacterial genome for DNA regions resembling a given TF-binding motif. PMID: 17085555. +is_a: ECO:0000028 ! motif similarity evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-09T14:12:54Z + +[Term] +id: ECO:0005533 +name: phylogenetic footprinting evidence +def: "A type of motif discovery evidence based on a set of algorithms that infer motifs in non-coding DNA sequences from the conservation of functional regulatory sites through evolution at a higher rate than their local surroundings for the identification of an evolutionary 'footprint'." [ECO:SW, PMID:16477324, PMID:3199442] +comment: The name derives to experimental footprinting techniques where transcription factor (TF)-binding sites are identified by sequencing the region of DNA protected from biochemical agents by the bound TF. This technique is used to identify or verify transcription factor-binding motifs, most commonly in the Eukaryota (PMID: 11997340). +is_a: ECO:0005531 ! motif discovery evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-09-30T16:23:02Z + +[Term] +id: ECO:0005534 +name: comparative genomics motif search evidence +def: "A type of motif similarity evidence based on comparative genomics which uses a search method to scan a set of genomes for instances of a specific motif, and then applies a comparative criterion to analyze the collective strength of these predictions, based on the assumption that functional instances of the motif will be preserved by natural selection and their prediction will therefore be consistent across genomes." [ECO:SW, PMID:10854408] +comment: This technique is used to predict transcription factor binding sites in gene promoter regions by identifying conserved DNA regions across multiple genomes. PMID: 22305460. Orthology is a typical comparative criterion used. +is_a: ECO:0000028 ! motif similarity evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-09T14:30:38Z + +[Term] +id: ECO:0005535 +name: machine learning prediction of motif instance evidence +def: "A type of match to sequence model evidence which captures the application of a broad suite of supervised and unsupervised machine learning algorithms trained on known motif instances and applied to the prediction of novel putative instances of a motif in a set of biological sequences." [ECO:SW, PMID:17117497, PMID:2014171] +comment: Machine learning algorithms include, but are not limited to artificial neural networks, random-forest, k-means clustering, hidden Markov models. This suite of methods can be used to identify or verify transcription factor-binding sites in genomic sequences (PMID: 15703297). +is_a: ECO:0000202 ! match to sequence model evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-01T11:58:36Z + +[Term] +id: ECO:0005536 +name: position-specific scoring matrix motif search evidence +def: "A type of motif similarity evidence where the motif is represented by a position-specific frequency matrix (PSFM) that specifies the frequency of each base at each position of the motif, from which a position-specific scoring matrix (PSSM) can be derived under the framework of a log-likelihood ratio providing a scoring system based on the likelihood of a sequence given the motif, versus a genomic-frequency based null hypothesis." [ECO:SW, PMID:10812473] +comment: This technique is used to predict transcription factor binding sites in gene promoter regions by using a position weight matrix approach. (PMID: 15604457). +synonym: "PSSM" NARROW [] +synonym: "PSSM motif search" EXACT [] +is_a: ECO:0000028 ! motif similarity evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-09T15:04:35Z + +[Term] +id: ECO:0005537 +name: xylE reporter gene assay evidence +def: "A type of reporter gene assay evidence based on the fusion of the xylE gene to a specific promoter for the expression of catechol 2,3-dioxygenase that converts the colorless catechol substrate to yellow 2-hydroxymuconic semialdehyde for identification of the expression of a particular gene." [ECO:SW, PMID:2592344] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2015-10-28T15:31:28Z + +[Term] +id: ECO:0005538 +name: computationally derived logical inference +is_obsolete: true +created_by: snadendla +creation_date: 2015-11-17T15:15:50Z + +[Term] +id: ECO:0005539 +name: computationally derived logical inference used in automatic assertion +is_obsolete: true +created_by: snadendla +creation_date: 2015-11-17T15:17:47Z + +[Term] +id: ECO:0005540 +name: computationally derived logical inference from automatic assertion used in automatic assertion +is_obsolete: true +created_by: snadendla +creation_date: 2015-11-17T15:24:42Z + +[Term] +id: ECO:0005541 +name: computationally derived logical inference from manual assertion used in automatic assertion +is_obsolete: true +created_by: snadendla +creation_date: 2015-11-17T15:32:03Z + +[Term] +id: ECO:0005542 +name: biological system reconstruction evidence by experimental evidence from single species used in manual assertion +def: "A type of biological system reconstruction evidence by experimental evidence from single species that is used in a manual assertion." [ECO:SN, PMID:19450514] +comment: Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof. +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0005553 ! biological system reconstruction evidence by experimental evidence from single species +intersection_of: ECO:0005553 ! biological system reconstruction evidence by experimental evidence from single species +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: snadendla +creation_date: 2015-12-16T15:32:41Z + +[Term] +id: ECO:0005543 +name: biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion +def: "A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in a manual assertion." [ECO:SN, PMID:22232657] +comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments. +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0005552 ! biological system reconstruction evidence by experimental evidence from mixed species +intersection_of: ECO:0005552 ! biological system reconstruction evidence by experimental evidence from mixed species +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: snadendla +creation_date: 2015-12-16T15:45:01Z + +[Term] +id: ECO:0005544 +name: biological system reconstruction evidence based on orthology evidence used in manual assertion +def: "A type of biological system reconstruction evidence based on orthology evidence that is used in a manual assertion." [ECO:SN] +comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. +is_a: ECO:0005545 ! biological system reconstruction evidence based on orthology evidence +is_a: ECO:0005610 ! biological system reconstruction evidence based on homology evidence used in manual assertion +intersection_of: ECO:0005545 ! biological system reconstruction evidence based on orthology evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: snadendla +creation_date: 2015-12-18T16:20:49Z + +[Term] +id: ECO:0005545 +name: biological system reconstruction evidence based on orthology evidence +def: "A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by orthology from an existing experimentally supported model to a process, pathway or complex in another species." [ECO:SN, PMID:15660128] +comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. +is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence +created_by: snadendla +creation_date: 2015-12-16T15:57:37Z + +[Term] +id: ECO:0005546 +name: biological system reconstruction evidence based on paralogy evidence used in manual assertion +def: "A type of biological system reconstruction evidence based on paralogy evidence that is used in a manual assertion." [ECO:SN, PMID:15660128] +comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. +is_a: ECO:0005550 ! biological system reconstruction evidence based on paralogy evidence +is_a: ECO:0005610 ! biological system reconstruction evidence based on homology evidence used in manual assertion +intersection_of: ECO:0005550 ! biological system reconstruction evidence based on paralogy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: snadendla +creation_date: 2015-12-16T16:05:15Z + +[Term] +id: ECO:0005547 +name: biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion +def: "A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in a manual assertion." [ECO:SN, PMID:17876790] +comment: Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components. +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0005548 ! biological system reconstruction evidence based on inference from background scientific knowledge +intersection_of: ECO:0005548 ! biological system reconstruction evidence based on inference from background scientific knowledge +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: snadendla +creation_date: 2015-12-16T16:07:42Z + +[Term] +id: ECO:0005548 +name: biological system reconstruction evidence based on inference from background scientific knowledge +def: "A type of biological system reconstruction based solely on background scientific knowledge of that biological system." [ECO:SN, PMID:17876790] +comment: The available knowledge is usually a combination of partial or weak experimental evidence where either some components are missing or no physical interaction evidence can be found but the system is inferred by similarity to related systems in taxonomically-disparate organisms with experimental evidence. Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components. +is_a: ECO:0000088 ! biological system reconstruction evidence +created_by: snadendla +creation_date: 2015-12-18T15:32:15Z + +[Term] +id: ECO:0005549 +name: biological system reconstruction evidence based on homology evidence +def: "A type of biological system reconstruction where the evidence is inferred by homology based on conservation of sequence, function, and composition from an existing experimentally supported model to a process, pathway, or complex." [ECO:SN, PMID:15660128] +comment: Inference may be based on paralogy and/or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments in the same or another species. +is_a: ECO:0000088 ! biological system reconstruction evidence +created_by: snadendla +creation_date: 2015-12-18T15:49:46Z + +[Term] +id: ECO:0005550 +name: biological system reconstruction evidence based on paralogy evidence +def: "A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by paralogy from an existing experimentally supported model to a process, pathway, or complex in the same species." [ECO:SN, PMID:15660128] +comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments. +is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence +created_by: snadendla +creation_date: 2015-12-18T16:24:08Z + +[Term] +id: ECO:0005551 +name: biological system reconstruction evidence by experimental evidence +def: "A type of biological system reconstruction evidence that uses experimental evidence as support." [ECO:SN, PMID:19450514, PMID:22232657] +is_a: ECO:0000088 ! biological system reconstruction evidence +created_by: snadendla +creation_date: 2015-12-18T16:34:13Z + +[Term] +id: ECO:0005552 +name: biological system reconstruction evidence by experimental evidence from mixed species +def: "A type of biological system reconstruction evidence where the experimental evidence is derived by using a mix of species used in the same experiment." [ECO:SN, PMID:22232657] +comment: Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments. +is_a: ECO:0005551 ! biological system reconstruction evidence by experimental evidence +created_by: snadendla +creation_date: 2015-12-18T16:35:13Z + +[Term] +id: ECO:0005553 +name: biological system reconstruction evidence by experimental evidence from single species +def: "A type of biological system reconstruction where the experimental evidence is derived by using multiple experiments in a single species." [ECO:SN] +comment: Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof. +is_a: ECO:0005551 ! biological system reconstruction evidence by experimental evidence +created_by: snadendla +creation_date: 2015-12-18T16:36:44Z + +[Term] +id: ECO:0005554 +name: pairwise sequence alignment evidence +def: "A type of sequence alignment evidence where two sequences are aligned to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences (protein or nucleic acid)." [ECO:SN, url:http\://www.ebi.ac.uk/Tools/psa/] +is_a: ECO:0000200 ! sequence alignment evidence +created_by: snadendla +creation_date: 2016-02-08T14:30:46Z + +[Term] +id: ECO:0005555 +name: multiple sequence alignment evidence +def: "A type of sequence alignment evidence where three or more biological sequences (protein or nucleic acid) of similar length are aligned to infer homology and the evolutionary relationships between the sequences studied." [ECO:SN, url:http\://www.ebi.ac.uk/Tools/msa/] +is_a: ECO:0000200 ! sequence alignment evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2016-02-08T14:31:24Z + +[Term] +id: ECO:0005556 +name: multiple sequence alignment evidence used in manual assertion +def: "A type of multiple sequence alignment evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion +is_a: ECO:0005555 ! multiple sequence alignment evidence +intersection_of: ECO:0005555 ! multiple sequence alignment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISA" xsd:string +created_by: snadendla +creation_date: 2016-02-08T14:59:18Z + +[Term] +id: ECO:0005557 +name: multiple sequence alignment evidence used in automatic assertion +def: "A type of multiple sequence alignment evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0000248 {is_inferred="true"} ! sequence alignment evidence used in automatic assertion +is_a: ECO:0005555 ! multiple sequence alignment evidence +intersection_of: ECO:0005555 ! multiple sequence alignment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-02-08T15:00:50Z + +[Term] +id: ECO:0005558 +name: motif discovery evidence used in manual assertion +def: "A type of motif discovery evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005531 ! motif discovery evidence +is_a: ECO:0005556 {is_inferred="true"} ! multiple sequence alignment evidence used in manual assertion +intersection_of: ECO:0005531 ! motif discovery evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISA" xsd:string +created_by: snadendla +creation_date: 2016-02-08T15:07:47Z + +[Term] +id: ECO:0005559 +name: motif discovery evidence used in automatic assertion +def: "A type of motif discovery evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0005531 ! motif discovery evidence +is_a: ECO:0005557 {is_inferred="true"} ! multiple sequence alignment evidence used in automatic assertion +intersection_of: ECO:0005531 ! motif discovery evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-02-08T15:08:19Z + +[Term] +id: ECO:0005560 +name: pairwise sequence alignment evidence used in manual assertion +def: "A type of pairwise sequence alignment evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion +is_a: ECO:0005554 ! pairwise sequence alignment evidence +intersection_of: ECO:0005554 ! pairwise sequence alignment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISA" xsd:string +created_by: snadendla +creation_date: 2016-02-08T15:17:05Z + +[Term] +id: ECO:0005561 +name: pairwise sequence alignment evidence used in automatic assertion +def: "A type of pairwise sequence alignment evidence that is used in an automatic evidence." [ECO:SN] +is_a: ECO:0000248 {is_inferred="true"} ! sequence alignment evidence used in automatic assertion +is_a: ECO:0005554 ! pairwise sequence alignment evidence +intersection_of: ECO:0005554 ! pairwise sequence alignment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-02-08T15:17:52Z + +[Term] +id: ECO:0005562 +name: in-gel protein kinase assay evidence +def: "A type of protein kinase assay evidence resulting from the evaluation of the phosphorylation activity of kinase on a gel-incorporated kinase substrate." [PMID:12372853] +is_a: ECO:0001558 ! protein kinase assay evidence +created_by: snadendla +creation_date: 2016-02-09T12:05:29Z + +[Term] +id: ECO:0005563 +name: macroscopic current trace evidence +def: "A type of voltage clamp recording evidence that involves recording trace of the current (pA) going through the analyzed ion channels determined from voltage clamp current recording." [ECO:SN, PMID:17938230, url:http\://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch03.pdf] +is_a: ECO:0005576 ! voltage clamp recording evidence +created_by: snadendla +creation_date: 2016-02-09T12:40:03Z + +[Term] +id: ECO:0005564 +name: current density evidence +def: "A type of electrophysiology assay evidence that involves measuring the amount of current (pA) going through the ion channels per unit of membrane." [ECO:SN, url:http\://circres.ahajournals.org/content/102/11/1298.full, url:http\://www.bem.fi/book/04/04.htm] +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: snadendla +creation_date: 2016-02-09T12:43:07Z + +[Term] +id: ECO:0005565 +name: single channel conductance evidence +is_obsolete: true +created_by: snadendla +creation_date: 2016-02-09T12:45:42Z + +[Term] +id: ECO:0005566 +name: sustained current evidence +def: "A type of electrophysiology assay evidence that involves the measurement of the current that persists after the fast and slow inactivation of the analyzed channels." [ECO:SN, url:http\://www.nature.com/ncomms/journal/v3/n2/full/ncomms1717.html] +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: snadendla +creation_date: 2016-02-09T12:46:54Z + +[Term] +id: ECO:0005567 +name: steady state activation curve evidence +is_obsolete: true +created_by: snadendla +creation_date: 2016-02-09T12:48:17Z + +[Term] +id: ECO:0005568 +name: steady state inactivation curve evidence +is_obsolete: true +created_by: snadendla +creation_date: 2016-02-09T12:50:45Z + +[Term] +id: ECO:0005569 +name: window current trace evidence +is_obsolete: true +created_by: snadendla +creation_date: 2016-02-09T12:57:16Z + +[Term] +id: ECO:0005570 +name: use dependence of inactivation evidence +def: "A type of electrophysiology assay evidence in which the amount of current remaining after a series of depolarization at different frequencies described as the normalized current related to the time course." [ECO:SN, url:http\://circres.ahajournals.org/content/89/8/700.full] +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: snadendla +creation_date: 2016-02-09T12:58:18Z + +[Term] +id: ECO:0005571 +name: current clamp recording evidence +def: "A type of electrophysiology assay evidence that involves recording the change in membrane potential caused by applying specific current to the cell." [ECO:SN, url:http\://www.nature.com/nprot/journal/v4/n8/full/nprot.2009.91.html] +synonym: "current clamp voltage recording evidence" EXACT [] +xref: birnlex:2278 "current clamp voltage recording protocol" +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: snadendla +creation_date: 2016-02-12T13:58:32Z + +[Term] +id: ECO:0005572 +name: whole-cell voltage clamp recording evidence +def: "A type of voltage clamp recording evidence that involves direct measurement of ionic current across a membrane while controlling the membrane potential by maintaining the voltage." [ECO:SN, url:http\://circres.ahajournals.org/content/72/1/91.full.pdf] +xref: birnlex:2281 "whole-cell voltage clamp recording protocol" +is_a: ECO:0005576 ! voltage clamp recording evidence +created_by: snadendla +creation_date: 2016-02-12T14:06:40Z + +[Term] +id: ECO:0005573 +name: cell-attached single-channel recording evidence +def: "A type of patch-clamp recording evidence that involves isolation of a patch of membrane electrically from the external solution and to record current flowing into the patch and this is achieved by pressing a fire-polished glass pipette, which has been filled with a suitable electrolyte solution, against the surface of a cell and applying light suction." [ECO:SN, url:http\://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch04.pdf] +synonym: "cell-attached patch-clamp recording evidence" EXACT [] +xref: birnlex:2283 "cell-attached single-channel recording protocol" +is_a: ECO:0006012 ! patch-clamp recording evidence +created_by: snadendla +creation_date: 2016-02-12T14:07:39Z + +[Term] +id: ECO:0005574 +name: cell-detached inside-out single-channel recording evidence +def: "A type of patch-clamp recording evidence that involves study of individual ion channels by gently pulling the patch of membrane away from the cell, and the patch remains attached to the pipette with its cytoplasmic surface exposed to the bathing solution." [ECO:SN, url:http\://www.acnp.org/g4/GN401000005/CH005.html] +synonym: "cell-detached inside-out patch-clamp recording evidence" EXACT [] +xref: birnlex:2286 "cell-detached inside-out single-channel recording protocol" +is_a: ECO:0006012 ! patch-clamp recording evidence +created_by: snadendla +creation_date: 2016-02-12T14:16:53Z + +[Term] +id: ECO:0005575 +name: reconstituted bilayer single-channel patch recording evidence +def: "A type of patch-clamp reocrding evidence that involves ion channel analysis by incorporating the ion channels into liposomes suitable for patch-clamping which allows isolation and study of a specific population of channels into that bilayer membrane." [ECO:SN, url:http\://nanion.de/images/stories/papers/Bilayers_lab-on-a-chip08.pdf] +xref: birnlex:2287 "reconstituted bilayer single-channel patch recording protocol" +is_a: ECO:0006012 ! patch-clamp recording evidence +created_by: snadendla +creation_date: 2016-02-12T14:17:46Z + +[Term] +id: ECO:0005576 +name: voltage clamp recording evidence +def: "A type of electrophysiology assay evidence that involves recording membrane potential by controlling the membrane voltage and measuring the transmembrane current required to maintain that voltage." [ECO:SN, url:http\://neurobio.drexelmed.edu/GaoWeb/Resources/Recording%20_Techniques.pdf, url:http\://www.acnp.org/g4/GN401000005/CH005.html] +synonym: "voltage clamp current recording evidence" EXACT [] +xref: birnlex:2279 "voltage clamp current recording protocol" +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: snadendla +creation_date: 2016-02-12T14:18:36Z + +[Term] +id: ECO:0005577 +name: electroencephalography recording evidence +def: "A type of electrophysiology assay evidence that involves recording the electrical activity of the brain by means of electrodes placed on the surface of the head." [ECO:SN, url:http\://www.jove.com/science-education/5420/electro-encephalography-eeg] +xref: birnlex:2293 "electroencephalography recording protocol" +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: snadendla +creation_date: 2016-02-12T14:19:21Z + +[Term] +id: ECO:0005578 +name: super-resolution microscopy evidence +def: "A type of microscopy evidence where the images are taken with a higher resolution than the diffraction limit that help in studying nanoscopic sub-cellular structures." [ECO:SN, url:http\://journal.frontiersin.org/article/10.3389/fncel.2015.00007/full] +is_a: ECO:0001098 ! microscopy evidence +created_by: snadendla +creation_date: 2016-02-12T15:20:47Z + +[Term] +id: ECO:0005579 +name: immunogold labelling evidence used in manual assertion +def: "A type of immunogold labelling evidence that is used in a manual assertion." [ECO:SN] +synonym: "immunogold labelling" BROAD [] +is_a: ECO:0000090 ! immunogold labelling evidence +is_a: ECO:0006031 {is_inferred="true"} ! immunolocalization evidence used in manual assertion +intersection_of: ECO:0000090 ! immunogold labelling evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-02-12T15:38:03Z + +[Term] +id: ECO:0005580 +name: flow cytometry evidence used in manual assertion +def: "A type of flow cytometry evidence that is used in a manual assertion." [ECO:SN] +synonym: "FCM" BROAD [] +is_a: ECO:0000268 ! flow cytometry evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000268 ! flow cytometry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-02-12T16:04:23Z + +[Term] +id: ECO:0005581 +name: enzyme-linked immunoabsorbent assay evidence used in manual assertion +def: "A type of enzyme-linked immunoabsorbent assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "ELISA evidence|enzyme-linked immunosorbent assay evidence" BROAD [] +is_a: ECO:0000267 ! enzyme-linked immunoabsorbent assay evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000267 ! enzyme-linked immunoabsorbent assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-02-12T16:23:38Z + +[Term] +id: ECO:0005582 +name: cell-detached outside-out single-channel recording evidence +def: "A type of patch-clamp recording evidence where a part of cellular membrane is detached from the cell and whose external face is exposed to the extracellular medium which allows isolating and studying a single or at least a small number of channels into that fragment (patch)." [ECO:SN, url:http\://www.nature.com/nprot/journal/v2/n11/full/nprot.2007.403.html] +synonym: "cell-detached outside-out patch-clamp evidence" EXACT [] +is_a: ECO:0006012 ! patch-clamp recording evidence +created_by: snadendla +creation_date: 2016-03-02T10:47:25Z + +[Term] +id: ECO:0005583 +name: cut-open oocyte voltage clamp recording evidence +def: "A type of voltage clamp recording evidence that involves inserting the oocyte in a chamber that separates the surface into 3 regions where the top portion of the oocyte membrane is the region that is clamped from which currents are actually recorded while the bottom portion is the region of the oocyte that is cut-open, making it possible to inject current intracellularly through a low resistance pathway." [ECO:SN, PMID:9711615] +is_a: ECO:0005576 ! voltage clamp recording evidence +created_by: snadendla +creation_date: 2016-03-02T11:29:32Z + +[Term] +id: ECO:0005584 +name: macropatch voltage clamp recording evidence +def: "A type of voltage clamp recording evidence where recording is done on a fragment of the cell membrane (attached to or detached from the cell) using a large tip diameter pipette that allows isolating and studying a small number of channels into that fragment (patch)." [ECO:SN, url:http\://jxb.oxfordjournals.org/content/50/Special_Issue/1037.full.pdf] +is_a: ECO:0005576 ! voltage clamp recording evidence +created_by: snadendla +creation_date: 2016-03-02T11:33:48Z + +[Term] +id: ECO:0005585 +name: high throughput mass spectrometry evidence +def: "A type of mass spectrometry evidence where mass-to-charge ratio (m/z) of gas-phase ions is measured which is used to identify and quantify molecules in complex solutions within the framework of high throughput studies." [ECO:SN, PMID:20805795] +comment: There are significant differences in data analysis and data evaluation if to compare a HTP mass-spec and a regular mass-spec. The regular mass spec method is quite a reliable technique for identifying a protein molecular weight and analysing its post-modifications, other mass-spec applications might require the additional quality controls and more stringent data evaluation. More complex sample mixture is processed, more quality questions should be addressed, such as a sample contamination and sensitivity of the mass spectrometer while using HTP mass-spec.\nFor HTP mass-spec, the analysis of the small data sets allows to manually assign the spectra to proteins or peptides, but for large data sets only the statistical tools can be used instead. +is_a: ECO:0001096 ! mass spectrometry evidence +created_by: snadendla +creation_date: 2016-03-02T12:33:07Z + +[Term] +id: ECO:0005586 +name: high throughput mass spectrometry evidence used in manual assertion +def: "A type of high throughput mass spectrometry evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion +is_a: ECO:0005585 ! high throughput mass spectrometry evidence +intersection_of: ECO:0005585 ! high throughput mass spectrometry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-03-02T12:50:02Z + +[Term] +id: ECO:0005587 +name: confocal microscopy evidence +def: "A type of microscopy evidence where sharp images are taken by excluding most of the light from the specimen that is not from the microscope's focal plane, thus allowing better contrast and less haze, which makes it possible to build three-dimensional reconstructions of a volume of the specimen by assembling a series of thin slices taken along the vertical axis." [ECO:SN, url:http\://www.physics.emory.edu/faculty/weeks//lab/papers/ebbe05.pdf] +is_a: ECO:0001098 ! microscopy evidence +created_by: snadendla +creation_date: 2016-03-17T12:29:45Z + +[Term] +id: ECO:0005588 +name: wide-field microscopy evidence +def: "A type of microscopy evidence where imaging of a thin cell or a tissue specimen is performed by full aperture emission of light gathered by the microscope's objective, which maximizes the recorded signal and simultaneously minimizes the required exposure times." [ECO:SN, url:http\://jcb.rupress.org/content/172/1/9.full, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048960/, url:http\://zeiss-campus.magnet.fsu.edu/articles/livecellimaging/techniques.html] +is_a: ECO:0001098 ! microscopy evidence +created_by: snadendla +creation_date: 2016-03-17T12:30:27Z + +[Term] +id: ECO:0005589 +name: confocal microscopy evidence used in manual assertion +def: "A type confocal microscopy evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion +is_a: ECO:0005587 ! confocal microscopy evidence +intersection_of: ECO:0005587 ! confocal microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-03-17T12:32:24Z + +[Term] +id: ECO:0005590 +name: wide-field microscopy evidence used in manual assertion +def: "A type of wide-field microscopy evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion +is_a: ECO:0005588 ! wide-field microscopy evidence +intersection_of: ECO:0005588 ! wide-field microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-03-17T12:34:42Z + +[Term] +id: ECO:0005591 +name: immunogold labelling electron microscopy assay evidence +def: "A type of immunogold labelling evidence where electron microscopy technique is used to detect the location of the antibodies labeled with colloidal gold particles." [ECO:SN, PMID:4110101] +is_a: ECO:0000090 ! immunogold labelling evidence +is_a: ECO:0005033 ! electron microscopy evidence +created_by: snadendla +creation_date: 2016-03-17T15:43:00Z + +[Term] +id: ECO:0005592 +name: immunogold labelling electron microscopy assay evidence used in manual assertion +def: "A type of immunogold labelling electron microscopy assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005579 {is_inferred="true"} ! immunogold labelling evidence used in manual assertion +is_a: ECO:0005591 ! immunogold labelling electron microscopy assay evidence +is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion +intersection_of: ECO:0005591 ! immunogold labelling electron microscopy assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-03-17T16:39:37Z + +[Term] +id: ECO:0005593 +name: immunodetection assay evidence +def: "A type of direct assay evidence that involves the use of antibodies to detect biomolecules." [ECO:SN] +is_a: ECO:0000002 ! direct assay evidence +created_by: snadendla +creation_date: 2016-03-17T17:07:33Z + +[Term] +id: ECO:0005594 +name: immunoperoxidase immunolocalization evidence +def: "A type of immunolocalization evidence involving enzymatic detection of a peroxidase tagged antibody." [ECO:SN] +is_a: ECO:0000087 ! immunolocalization evidence +created_by: snadendla +creation_date: 2016-03-17T17:11:34Z + +[Term] +id: ECO:0005595 +name: immunoperoxidase immunolocalization evidence used in manual assertion +def: "A type of immunoperoxidase immunolocalization evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005594 ! immunoperoxidase immunolocalization evidence +is_a: ECO:0006031 {is_inferred="true"} ! immunolocalization evidence used in manual assertion +intersection_of: ECO:0005594 ! immunoperoxidase immunolocalization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-03-17T17:14:05Z + +[Term] +id: ECO:0005596 +name: immunoperoxidase immunolocalization electron microscopy evidence +def: "A type of immunoperoxidase immunolocalization evidence where electron microscopy technique is used for enzymatic detection of a peroxidase tagged antibody." [ECO:SN] +synonym: "Immunoperoxidase labelling electron microscopy" EXACT [] +is_a: ECO:0005033 ! electron microscopy evidence +is_a: ECO:0005594 ! immunoperoxidase immunolocalization evidence +created_by: snadendla +creation_date: 2016-03-17T17:16:38Z + +[Term] +id: ECO:0005597 +name: immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion +def: "A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "immunoperoxidase labelling electron microscopy evidence" BROAD [] +is_a: ECO:0005595 {is_inferred="true"} ! immunoperoxidase immunolocalization evidence used in manual assertion +is_a: ECO:0005596 ! immunoperoxidase immunolocalization electron microscopy evidence +is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion +intersection_of: ECO:0005596 ! immunoperoxidase immunolocalization electron microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-03-17T17:23:56Z + +[Term] +id: ECO:0005598 +name: wide-field fluorescence microscopy evidence +def: "A type of wide-field microscopy evidence where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter." [ECO:SN, url:http\://www.bristol.ac.uk/synaptic/research/techniques/widefield.html] +comment: The optical filter is used to select the wavelength of excitation light. The detection camera is typically a CCD camera. Both topographical and dynamic information of a sample are obtained. +is_a: ECO:0005588 ! wide-field microscopy evidence +created_by: snadendla +creation_date: 2016-03-18T11:53:56Z + +[Term] +id: ECO:0005599 +name: immunofluorescence wide-field microscopy evidence +def: "A type of wide-field fluorescence microscopy evidence where fluorescently tagged antibodies are imaged that are used to bind to their antigens." [ECO:SN, url:http\://journals.plos.org/plosone/article?id=10.1371/journal.pone.0057135] +synonym: "wide-field epifluorescence microscopy" EXACT [] +is_a: ECO:0000007 ! immunofluorescence evidence +is_a: ECO:0005598 ! wide-field fluorescence microscopy evidence +created_by: snadendla +creation_date: 2016-03-18T11:55:40Z + +[Term] +id: ECO:0005600 +name: immunofluorescence confocal microscopy evidence +def: "A type of confocal microscopy evidence where light passes through immunofluorescent-stained tissue sections or cells and then the emitted light passes through the dichroic mirror and is focused onto the pinhole for subsequent detection." [ECO:SN, url:http\://www.physics.emory.edu/faculty/weeks/confocal/, url:https\://www.hycultbiotech.com/media/wysiwyg/protocol_Immunofluorescence.pdf] +comment: This allows visualization of sub cellular distribution of biomolecules of interest. +is_a: ECO:0000007 ! immunofluorescence evidence +is_a: ECO:0005587 ! confocal microscopy evidence +property_value: IAO:0000112 "The endogenous protein expression and localization for WalR and WalK was also checked using confocal immunofluorescence microscopy. WalR could be localized to the cytoplasm of B. anthracis but WalK could not be detected once again (data not shown)." xsd:string {xref="PMID:24490131"} +created_by: snadendla +creation_date: 2016-03-18T12:48:42Z + +[Term] +id: ECO:0005601 +name: immunofluorescence confocal microscopy evidence used in manual assertion +def: "A type of immunofluorescence confocal microscopy evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005589 {is_inferred="true"} ! confocal microscopy evidence used in manual assertion +is_a: ECO:0005600 ! immunofluorescence confocal microscopy evidence +is_a: ECO:0005804 {is_inferred="true"} ! immunofluorescence evidence used in manual assertion +intersection_of: ECO:0005600 ! immunofluorescence confocal microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-03-18T12:52:04Z + +[Term] +id: ECO:0005603 +name: protein mass spectrometry evidence +def: "A type of mass spectrometry evidence where one or more protein analyses are performed." [ECO:SN] +is_a: ECO:0001096 ! mass spectrometry evidence +created_by: snadendla +creation_date: 2016-03-18T13:11:37Z + +[Term] +id: ECO:0005604 +name: cross-streak test evidence +def: "A type of cell proliferation assay evidence where the investigated organism or a potential antagonist is inoculated so as to grow along the diameter line of the agar medium in a Petri dish, then, potentially susceptible microbes (usually bacteria) are streaked perpendicularly to the growth line and inhibition of growth of a test microbe in the vicinity of that line suggests that the antagonist secretes one or more diffusible antibiotics." [ECO:SN, url:www.jstor.org/stable/3792785] +is_a: ECO:0005007 ! cell proliferation assay evidence +created_by: snadendla +creation_date: 2016-03-29T15:40:54Z + +[Term] +id: ECO:0005605 +name: disk diffusion test evidence +def: "A type of zone of inhibition evidence where sensitivity/resistance to an antimicrobial agent is tested by impregnating small filter disks with a known concentration of antimicrobial agent which is then placed on a Mueller-Hinton agar plate inoculated with the test organism." [ECO:SN, url:http\://www.cdc.gov/nczved/resources/cholera/ch9.pdf] +is_a: ECO:0007083 ! zone of inhibition evidence +property_value: IAO:0000112 "Disk diffusion assays were used to determine if ohr and ohrR mutations affected resistance to ROS. The ohr mutant was less resistant than its parental strain when challenged with organic peroxides as shown by the zones of growth inhibition: 4.1 +- 0.2 cm for CuOOH and 3.1 +- 0.1 cm for tBOOH versus to 2.3 +- 0.2 and 2.5 +- 0.3 cm for wild type strain." xsd:string {xref="PMID:21569462"} +created_by: snadendla +creation_date: 2016-03-29T16:02:22Z + +[Term] +id: ECO:0005606 +name: cell transfection experiment evidence +def: "A type of experimental evidence where a foreign genetic material is introduced into the cell for study of gene function and regulation and protein function." [ECO:SN, url:http\://ch.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/transfection/, url:http\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911531/] +is_a: ECO:0000006 ! experimental evidence +created_by: snadendla +creation_date: 2016-03-29T16:59:17Z + +[Term] +id: ECO:0005607 +name: tethered cell assay evidence +def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by observing the rotational behavior of the cells tethered to a coverslip by their flagella." [ECO:SN, PMID:1103143] +is_a: ECO:0005021 ! chemotaxis assay evidence +created_by: snadendla +creation_date: 2016-03-31T15:51:10Z + +[Term] +id: ECO:0005608 +name: tumble frequency assay evidence +def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by measuring chemotaxis in response to sudden concentration changes from one uniform spatial environment to another." [ECO:SN, PMID:4576832] +synonym: "Temporal assay" EXACT [] +is_a: ECO:0005021 ! chemotaxis assay evidence +created_by: snadendla +creation_date: 2016-03-31T16:42:35Z + +[Term] +id: ECO:0005609 +name: capillary assay evidence +def: "A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by measuring the rate of accumulation of the cells into a capillary." [ECO:SN, PMID:4632978] +comment: To determine the presence of negative chemotaxis, a repellent is added to the organismal suspension (but not in the capillary) and the organisms that fled into the capillary are measured. +comment: To determine the presence of positive chemotaxis, an attractant is placed in the capillary and the presence of positive chemotaxis is determined based on the amount of organism that accumulated in the capillary. +is_a: ECO:0005021 ! chemotaxis assay evidence +created_by: snadendla +creation_date: 2016-03-31T16:45:29Z + +[Term] +id: ECO:0005610 +name: biological system reconstruction evidence based on homology evidence used in manual assertion +def: "A type of biological system reconstruction evidence based on homology evidence that is used in a manual assertion." [ECO:SN] +comment: Inference may be based on paralogy and/or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments in the same or another species. +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0005549 ! biological system reconstruction evidence based on homology evidence +intersection_of: ECO:0005549 ! biological system reconstruction evidence based on homology evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: snadendla +creation_date: 2016-03-31T17:00:03Z + +[Term] +id: ECO:0005611 +name: inference from experimental data evidence +def: "A type of direct assay evidence used in manual assertion where phenotype or disease association is made with the gene because of involvement in the molecular mechanism of disease or involvement in a pathway known to contribute to a disease." [ECO:SN] +synonym: "IED" EXACT [] +synonym: "inferred from experimental data" EXACT [] +is_obsolete: true +created_by: snadendla +creation_date: 2016-04-12T16:55:16Z + +[Term] +id: ECO:0005612 +name: inference from phenotype manipulation evidence +def: "A type of mutant phenotype evidence used in manual assertion where variations or changes, such as mutations or abnormal levels of the product(s) of a single gene of interest, including non-mutational changes such as inhibition with antibodies or other inhibitors are determined." [ECO:SN] +comment: Changes can include any gene mutation/knockout, over-expression or ectopic expression of wild-type or mutant genes, anti-sense experiments, RNAi experiments, specific protein inhibitors. +synonym: "inferred from phenotype manipulation" EXACT [] +synonym: "IPM" EXACT [] +is_obsolete: true +created_by: snadendla +creation_date: 2016-04-12T17:09:43Z + +[Term] +id: ECO:0005613 +name: inference by association of genotype from phenotype +def: "A type of natural variation mutant evidence used in manual assertion where alleles are directly linked to phenotype and Mendelian diseases as a result of various experiments." [ECO:SN] +comment: Used for 1) polymorphism or segregation of genetic markers (SNPs, mutations, RFLPs, microsatellites); 2) polymorphism or segregation of physical markers (FISH, centromeric or heterochromatic regions, chromosomal banding patterns); 3) detection of polymorphisms in inbred stock. +synonym: "IAGP" EXACT [] +synonym: "inferred by association of genotype from phenotype" EXACT [] +is_a: ECO:0001237 ! natural variation mutant evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-04-12T17:24:00Z + +[Term] +id: ECO:0005614 +name: two-dimensional polyacrylamide gel electrophoresis evidence used in manual assertion +def: "A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in a manual assertion." [ECO:SN] +synonym: "2D-PAGE evidence" BROAD [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005503 ! two-dimensional polyacrylamide gel electrophoresis evidence +intersection_of: ECO:0005503 ! two-dimensional polyacrylamide gel electrophoresis evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-05-06T11:03:08Z + +[Term] +id: ECO:0005615 +name: alkaline phosphatase reporter gene assay evidence used in manual assertion +def: "A type of alkaline phosphatase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "SEAP reporter assay" RELATED [] +synonym: "secreted embryonic alkaline phosphatase reporter assay" RELATED [] +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0001801 ! alkaline phosphatase reporter gene assay evidence +intersection_of: ECO:0001801 ! alkaline phosphatase reporter gene assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T11:05:59Z + +[Term] +id: ECO:0005616 +name: beta-galactosidase reporter gene assay evidence used in manual assertion +def: "A type of beta-galactosidase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "beta-gal reporter gene assay evidence" BROAD [] +synonym: "lacZ reporter gene assay" RELATED [] +synonym: "LacZ transcript localization evidence used in manual assertion" EXACT [] +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0001802 ! beta-galactosidase reporter gene assay evidence +intersection_of: ECO:0001802 ! beta-galactosidase reporter gene assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T11:08:57Z + +[Term] +id: ECO:0005617 +name: chloramphenicol acetyltransferase reporter gene assay evidence used in manual assertion +def: "A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "CAT reporter gene assay evidence" BROAD [] +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0001803 ! chloramphenicol acetyltransferase reporter gene assay evidence +intersection_of: ECO:0001803 ! chloramphenicol acetyltransferase reporter gene assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T11:11:07Z + +[Term] +id: ECO:0005618 +name: chromatin immunoprecipitation-chip evidence used in automatic assertion +def: "A type of chromatin immunoprecipitation-chip evidence that is used in an automatic assertion." [ECO:SN] +synonym: "ChIP-chip evidence" BROAD [] +synonym: "ChIP-on-chip evidence" BROAD [] +is_a: ECO:0000230 ! chromatin immunoprecipitation-chip evidence +is_a: ECO:0007786 {is_inferred="true"} ! chromatin immunoprecipitation evidence used in automatic assertion +intersection_of: ECO:0000230 ! chromatin immunoprecipitation-chip evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: snadendla +creation_date: 2016-05-06T11:17:04Z + +[Term] +id: ECO:0005619 +name: chromatin immunoprecipitation- exonuclease evidence used in automatic assertion +def: "A type of chromatin immunoprecipitation- exonuclease evidence that is used in an automatic assertion." [ECO:SN] +synonym: "ChIP-exo evidence" BROAD [] +is_a: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence +is_a: ECO:0007786 {is_inferred="true"} ! chromatin immunoprecipitation evidence used in automatic assertion +intersection_of: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: snadendla +creation_date: 2016-05-06T11:22:25Z + +[Term] +id: ECO:0005620 +name: chromatin immunoprecipitation-PCR evidence used in manual assertion +def: "A type of chromatin immunoprecipitation-PCR evidence that is used in a manual assertion." [ECO:SN] +synonym: "ChIP-PCR evidence" BROAD [] +is_a: ECO:0000227 ! chromatin immunoprecipitation-PCR evidence +is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion +intersection_of: ECO:0000227 ! chromatin immunoprecipitation-PCR evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T11:25:01Z + +[Term] +id: ECO:0005621 +name: chromatin immunoprecipitation-seq evidence used in automatic assertion +def: "A type of chromatin immunoprecipitation-seq evidence that is used in an automatic assertion." [ECO:SN] +synonym: "ChIP-SEQ evidence" BROAD [] +synonym: "ChIP-seq evidence" BROAD [] +is_a: ECO:0000229 ! chromatin immunoprecipitation-seq evidence +is_a: ECO:0007786 {is_inferred="true"} ! chromatin immunoprecipitation evidence used in automatic assertion +intersection_of: ECO:0000229 ! chromatin immunoprecipitation-seq evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: snadendla +creation_date: 2016-05-06T11:29:13Z + +[Term] +id: ECO:0005622 +name: comparative genomics motif search evidence used in manual assertion +def: "A type of comparative genomics motif search evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion +is_a: ECO:0005534 ! comparative genomics motif search evidence +intersection_of: ECO:0005534 ! comparative genomics motif search evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: snadendla +creation_date: 2016-05-06T14:49:36Z + +[Term] +id: ECO:0005623 +name: comparative genomics motif search evidence used in automatic assertion +def: "A type of comparative genomics motif search evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion +is_a: ECO:0005534 ! comparative genomics motif search evidence +intersection_of: ECO:0005534 ! comparative genomics motif search evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-05-06T14:52:21Z + +[Term] +id: ECO:0005624 +name: consensus search evidence used in manual assertion +def: "A type of consensus search evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion +is_a: ECO:0005532 ! consensus search evidence +intersection_of: ECO:0005532 ! consensus search evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: snadendla +creation_date: 2016-05-06T14:54:53Z + +[Term] +id: ECO:0005625 +name: consensus search evidence used in automatic assertion +def: "A type of consensus search evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion +is_a: ECO:0005532 ! consensus search evidence +intersection_of: ECO:0005532 ! consensus search evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-05-06T14:58:22Z + +[Term] +id: ECO:0005626 +name: copper-phenanthroline footprinting evidence used in manual assertion +def: "A type of copper-phenanthroline footprinting evidence that is used in a manual assertion." [ECO:SN] +synonym: "1,10-phenanthroline-copper footprinting evidence" BROAD [] +synonym: "OP-Cu Complex" RELATED [] +is_a: ECO:0001815 ! copper-phenanthroline footprinting evidence +is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion +intersection_of: ECO:0001815 ! copper-phenanthroline footprinting evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T15:01:25Z + +[Term] +id: ECO:0005627 +name: DNA adenine methyltransferase identification evidence used in manual assertion +def: "A type of DNA adenine methyltransferase identification evidence that is used in a manual assertion." [ECO:SN] +synonym: "DamID evidence" BROAD [] +is_a: ECO:0001824 ! DNA adenine methyltransferase identification evidence +is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion +intersection_of: ECO:0001824 ! DNA adenine methyltransferase identification evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T15:05:14Z + +[Term] +id: ECO:0005628 +name: DNA adenine methyltransferase identification evidence used in automatic assertion +def: "A type of DNA adenine methyltransferase identification evidence that is used in an automatic assertion." [ECO:SN] +synonym: "DamID evidence" BROAD [] +is_a: ECO:0001824 ! DNA adenine methyltransferase identification evidence +is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion +intersection_of: ECO:0001824 ! DNA adenine methyltransferase identification evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: snadendla +creation_date: 2016-05-06T15:06:46Z + +[Term] +id: ECO:0005629 +name: DNA affinity chromatography evidence used in manual assertion +def: "A type of DNA affinity chromatography evidence that is used in a manual assertion." [ECO:SN] +synonym: "DNA affinity purification evidence" BROAD [] +is_a: ECO:0001809 ! DNA affinity chromatography evidence +is_a: ECO:0007680 {is_inferred="true"} ! chromatography evidence used in manual assertion +intersection_of: ECO:0001809 ! DNA affinity chromatography evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T15:08:40Z + +[Term] +id: ECO:0005630 +name: cDNA to DNA expression microarray evidence used in automatic assertion +def: "A type of cDNA to DNA expression microarray evidence that is used in an automatic assertion." [ECO:SN] +synonym: "DNA microarray evidence" BROAD [] +synonym: "RNA microarray evidence" BROAD [] +is_a: ECO:0000097 ! cDNA to DNA expression microarray evidence +is_a: ECO:0007301 {is_inferred="true"} ! expression microarray evidence used in automatic assertion +intersection_of: ECO:0000097 ! cDNA to DNA expression microarray evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: snadendla +creation_date: 2016-05-06T15:31:51Z + +[Term] +id: ECO:0005631 +name: DNAse footprinting evidence used in manual assertion +def: "A type of DNAse footprinting evidence that is used in a manual assertion." [ECO:SN] +synonym: "DNase protection evidence" BROAD [] +is_a: ECO:0001810 ! DNAse footprinting evidence +is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion +intersection_of: ECO:0001810 ! DNAse footprinting evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T15:35:41Z + +[Term] +id: ECO:0005632 +name: fluorescence anisotropy evidence used in manual assertion +def: "A type of fluorescence anisotropy evidence that is used in a manual assertion." [ECO:SN] +synonym: "FA evidence" BROAD [] +synonym: "fluorescence polarization" RELATED [] +synonym: "FP" RELATED [] +is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion +is_a: ECO:0001811 ! fluorescence anisotropy evidence +intersection_of: ECO:0001811 ! fluorescence anisotropy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-05-06T15:45:40Z + +[Term] +id: ECO:0005633 +name: ferric uptake regulator titration assay evidence used in manual assertion +def: "A type of ferric uptake regulator titration assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "FURTA evidence" BROAD [] +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0001829 ! ferric uptake regulator titration assay evidence +intersection_of: ECO:0001829 ! ferric uptake regulator titration assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T15:49:18Z + +[Term] +id: ECO:0005634 +name: genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion +def: "A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in a manual assertion." [ECO:SN] +synonym: "genomic SELEX evidence" BROAD [] +is_a: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence +is_a: ECO:0006088 {is_inferred="true"} ! systematic evolution of ligands by exponential amplification evidence used in manual assertion +intersection_of: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T16:41:30Z + +[Term] +id: ECO:0005635 +name: genomic systematic evolution of ligands by exponential amplification evidence used in automatic assertion +def: "A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion." [ECO:SN] +synonym: "genomic SELEX evidence" BROAD [] +is_a: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence +is_a: ECO:0007510 {is_inferred="true"} ! systematic evolution of ligands by exponential amplification evidence used in automatic assertion +intersection_of: ECO:0001812 ! genomic systematic evolution of ligands by exponential amplification evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: snadendla +creation_date: 2016-05-06T16:43:03Z + +[Term] +id: ECO:0005636 +name: green fluorescent protein reporter gene assay evidence used in manual assertion +def: "A type of green fluorescent protein reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "GFP promoter fusion" RELATED [] +synonym: "GFP reporter gene assay evidence" BROAD [] +synonym: "green fluorescent protein promoter fusion" RELATED [] +synonym: "green fluorescent protein transcript localization evidence used in manual assertion" EXACT [] +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0001816 ! green fluorescent protein reporter gene assay evidence +intersection_of: ECO:0001816 ! green fluorescent protein reporter gene assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T16:46:32Z + +[Term] +id: ECO:0005637 +name: green fluorescent protein reporter gene assay evidence used in automatic assertion +def: "A type of green fluorescent protein reporter gene assay evidence that is used in an automatic assertion." [ECO:SN] +synonym: "GFP promoter fusion" RELATED [] +synonym: "GFP reporter gene assay evidence" BROAD [] +synonym: "green fluorescent protein promoter fusion" RELATED [] +synonym: "green fluorescent protein transcript localization evidence used in automatic assertion" EXACT [] +is_a: ECO:0001816 ! green fluorescent protein reporter gene assay evidence +is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion +intersection_of: ECO:0001816 ! green fluorescent protein reporter gene assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: snadendla +creation_date: 2016-05-06T16:48:29Z + +[Term] +id: ECO:0005638 +name: cell growth regulation assay evidence used in manual assertion +def: "A type of cell growth regulation assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "growth curve analysis" RELATED [] +is_a: ECO:0000095 ! cell growth regulation assay evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000095 ! cell growth regulation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: snadendla +creation_date: 2016-05-06T16:52:15Z + +[Term] +id: ECO:0005639 +name: cell growth regulation assay evidence used in automatic assertion +def: "A type of cell growth regulation assay evidence that is used in an automatic assertion." [ECO:SN] +synonym: "growth curve analysis" RELATED [] +is_a: ECO:0000095 ! cell growth regulation assay evidence +is_a: ECO:0007778 {is_inferred="true"} ! cell growth assay evidence used in automatic assertion +intersection_of: ECO:0000095 ! cell growth regulation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: snadendla +creation_date: 2016-05-06T16:53:28Z + +[Term] +id: ECO:0005640 +name: glutathione S-transferase pull-down assay evidence used in manual assertion +def: "A type of glutathione S-transferase pull-down assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "GST pull-down assay evidence" BROAD [] +is_a: ECO:0001817 ! glutathione S-transferase pull-down assay evidence +is_a: ECO:0006077 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in manual assertion +intersection_of: ECO:0001817 ! glutathione S-transferase pull-down assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T16:56:39Z + +[Term] +id: ECO:0005641 +name: beta-glucuronidase reporter gene assay evidence used in manual assertion +def: "A type of beta-glucuronidase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "GUS reporter gene assay evidence" BROAD [] +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0001804 ! beta-glucuronidase reporter gene assay evidence +intersection_of: ECO:0001804 ! beta-glucuronidase reporter gene assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T16:58:29Z + +[Term] +id: ECO:0005642 +name: heteronuclear single quantum coherence spectroscopy evidence used in manual assertion +def: "A type of heteronuclear single quantum coherence spectroscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "HSQC evidence" BROAD [] +is_a: ECO:0001813 ! heteronuclear single quantum coherence spectroscopy evidence +is_a: ECO:0006165 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in manual assertion +intersection_of: ECO:0001813 ! heteronuclear single quantum coherence spectroscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:10:13Z + +[Term] +id: ECO:0005643 +name: hydroxyl-radical footprinting evidence used in manual assertion +def: "A type of hydroxyl-radical footprinting evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001818 ! hydroxyl-radical footprinting evidence +is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion +intersection_of: ECO:0001818 ! hydroxyl-radical footprinting evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:14:48Z + +[Term] +id: ECO:0005644 +name: immunoprecipitation evidence used in manual assertion +def: "A type of immunoprecipitation evidence that is used in a manual assertion." [ECO:SN] +synonym: "immunoprecipitation" BROAD [] +is_a: ECO:0000085 ! immunoprecipitation evidence +is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion +intersection_of: ECO:0000085 ! immunoprecipitation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:20:49Z + +[Term] +id: ECO:0005645 +name: interferometric reflectance imaging sensor evidence used in manual assertion +def: "A type of interferometric reflectance imaging sensor evidence that is used in a manual assertion." [ECO:SN] +synonym: "IRIS evidence" BROAD [] +synonym: "spectral reflectance imaging biosensor evidence" BROAD [] +synonym: "SRIB evidence" BROAD [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0005520 ! interferometric reflectance imaging sensor evidence +intersection_of: ECO:0005520 ! interferometric reflectance imaging sensor evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:27:58Z + +[Term] +id: ECO:0005646 +name: interferometric reflectance imaging sensor evidence used in automatic assertion +def: "A type of interferometric reflectance imaging sensor evidence that is used in an automatic assertion." [ECO:SN] +synonym: "IRIS evidence" BROAD [] +synonym: "spectral reflectance imaging biosensor evidence" BROAD [] +synonym: "SRIB evidence" BROAD [] +is_a: ECO:0005520 ! interferometric reflectance imaging sensor evidence +is_a: ECO:0007816 {is_inferred="true"} ! molecule detection assay evidence used in automatic assertion +intersection_of: ECO:0005520 ! interferometric reflectance imaging sensor evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: snadendla +creation_date: 2016-05-06T23:29:23Z + +[Term] +id: ECO:0005647 +name: isothermal titration calorimetry evidence used in manual assertion +def: "A type of isothermal titration calorimetry evidence that is used in a manual assertion." [ECO:SN] +synonym: "ITC evidence" BROAD [] +is_a: ECO:0001825 ! isothermal titration calorimetry evidence +is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion +intersection_of: ECO:0001825 ! isothermal titration calorimetry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:36:26Z + +[Term] +id: ECO:0005648 +name: luciferase reporter gene assay evidence used in manual assertion +def: "A type of luciferase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0001805 ! luciferase reporter gene assay evidence +intersection_of: ECO:0001805 ! luciferase reporter gene assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:44:52Z + +[Term] +id: ECO:0005649 +name: machine learning prediction of motif instance evidence used in manual assertion +def: "A type of machine learning prediction of motif instance evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000255 {is_inferred="true"} ! match to sequence model evidence used in manual assertion +is_a: ECO:0005535 ! machine learning prediction of motif instance evidence +intersection_of: ECO:0005535 ! machine learning prediction of motif instance evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:51:30Z + +[Term] +id: ECO:0005650 +name: machine learning prediction of motif instance evidence used in automatic assertion +def: "A type of machine learning prediction of motif instance evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0000256 {is_inferred="true"} ! match to sequence model evidence used in automatic assertion +is_a: ECO:0005535 ! machine learning prediction of motif instance evidence +intersection_of: ECO:0005535 ! machine learning prediction of motif instance evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-05-06T23:54:00Z + +[Term] +id: ECO:0005651 +name: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in automatic assertion +def: "A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in an automatic assertion." [ECO:SN] +synonym: "MALDI-TOF mass spectrometry evidence" BROAD [] +synonym: "MALDI-TOF-MS evidence" BROAD [] +is_a: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence +is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion +intersection_of: ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: snadendla +creation_date: 2016-05-06T23:57:30Z + +[Term] +id: ECO:0005652 +name: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in manual assertion +def: "A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in a manual assertion." [ECO:SN] +synonym: "Methidiumpropyl-EDTA Fe(II) footprinting evidence" BROAD [] +synonym: "MPE Fe(II) footprinting" RELATED [] +synonym: "MPE-EDTA Fe(II) footprinting evidence" BROAD [] +is_a: ECO:0001814 ! methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence +is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion +intersection_of: ECO:0001814 ! methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-09T14:42:28Z + +[Term] +id: ECO:0005653 +name: northern assay evidence used in manual assertion +def: "A type of northern assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "northern assay evidence" BROAD [] +synonym: "RNA blot evidence" BROAD [] +synonym: "transcript levels (e.g. Northerns)" RELATED [] +is_a: ECO:0000106 ! northern blot evidence +is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion +intersection_of: ECO:0000106 ! northern blot evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T14:48:03Z + +[Term] +id: ECO:0005654 +name: phylogenetic footprinting evidence used in manual assertion +def: "A type of phylogenetic footprinting evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005533 ! phylogenetic footprinting evidence +is_a: ECO:0005558 {is_inferred="true"} ! motif discovery evidence used in manual assertion +intersection_of: ECO:0005533 ! phylogenetic footprinting evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISA" xsd:string +created_by: snadendla +creation_date: 2016-05-09T14:50:52Z + +[Term] +id: ECO:0005655 +name: phylogenetic footprinting evidence used in automatic assertion +def: "A type of phylogenetic footprinting evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0005533 ! phylogenetic footprinting evidence +is_a: ECO:0005559 {is_inferred="true"} ! motif discovery evidence used in automatic assertion +intersection_of: ECO:0005533 ! phylogenetic footprinting evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-05-09T14:51:55Z + +[Term] +id: ECO:0005656 +name: methylation interference footprinting evidence used in manual assertion +def: "A type of methylation interference footprinting evidence that is used in a manual assertion." [ECO:SN] +synonym: "premethylation interference footprinting evidence used in manual assertion" EXACT [] +is_a: ECO:0001827 ! methylation interference footprinting evidence +is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion +intersection_of: ECO:0001827 ! methylation interference footprinting evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-09T14:55:25Z + +[Term] +id: ECO:0005657 +name: primer extension assay evidence used in manual assertion +def: "A type of primer extension assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion +is_a: ECO:0001819 ! primer extension assay evidence +intersection_of: ECO:0001819 ! primer extension assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T14:58:38Z + +[Term] +id: ECO:0005658 +name: position-specific scoring matrix motif search evidence used in manual assertion +def: "A type of position-specific scoring matrix motif search evidence that is used in a manual assertion." [ECO:SN] +synonym: "PSSM motif search evidence" BROAD [] +is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion +is_a: ECO:0005536 ! position-specific scoring matrix motif search evidence +intersection_of: ECO:0005536 ! position-specific scoring matrix motif search evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: snadendla +creation_date: 2016-05-09T15:01:31Z + +[Term] +id: ECO:0005659 +name: position-specific scoring matrix motif search evidence used in automatic assertion +def: "A type of position-specific scoring matrix motif search evidence that is used in an automatic assertion." [ECO:SN] +synonym: "PSSM motif search evidence" BROAD [] +is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion +is_a: ECO:0005536 ! position-specific scoring matrix motif search evidence +intersection_of: ECO:0005536 ! position-specific scoring matrix motif search evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-05-09T15:02:31Z + +[Term] +id: ECO:0005660 +name: quantitative polymerase chain reaction evidence used in manual assertion +def: "A type of quantitative polymerase chain reaction evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000231 ! quantitative polymerase chain reaction evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000231 ! quantitative polymerase chain reaction evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T15:09:01Z + +[Term] +id: ECO:0005661 +name: rapid amplification of cDNA ends polymerase chain reaction evidence used in manual assertion +def: "A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in a manual assertion." [ECO:SN] +synonym: "RACE PCR evidence" BROAD [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001820 ! rapid amplification of cDNA ends polymerase chain reaction evidence +intersection_of: ECO:0001820 ! rapid amplification of cDNA ends polymerase chain reaction evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T15:19:20Z + +[Term] +id: ECO:0005662 +name: regular expression motif search evidence used in manual assertion +def: "A type of regular expression motif search evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000257 {is_inferred="true"} ! motif similarity evidence used in manual assertion +is_a: ECO:0005505 ! regular expression motif search evidence +intersection_of: ECO:0005505 ! regular expression motif search evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISM" xsd:string +created_by: snadendla +creation_date: 2016-05-09T16:13:55Z + +[Term] +id: ECO:0005663 +name: regular expression motif search evidence used in automatic assertion +def: "A type of regular expression motif search evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0000258 {is_inferred="true"} ! motif similarity evidence used in automatic assertion +is_a: ECO:0005505 ! regular expression motif search evidence +intersection_of: ECO:0005505 ! regular expression motif search evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla +creation_date: 2016-05-09T16:15:03Z + +[Term] +id: ECO:0005664 +name: RNA sequencing assay evidence used in manual assertion +comment: Use ECO:0006068 (RNA-seq evidence used in manual assertion) in place of this term. +is_obsolete: true +created_by: snadendla +creation_date: 2016-05-09T16:18:35Z + +[Term] +id: ECO:0005665 +name: RNA sequencing assay evidence used in automatic assertion +comment: Use ECO:0006069 (RNA-seq evidence used in automatic assertion) in place of this term. +is_obsolete: true +created_by: snadendla +creation_date: 2016-05-09T16:19:38Z + +[Term] +id: ECO:0005666 +name: S1 nuclease protection assay evidence used in manual assertion +def: "A type of S1 nuclease protection assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001240 {is_inferred="true"} ! nuclease protection assay evidence used in manual assertion +is_a: ECO:0005521 ! S1 nuclease protection assay evidence +intersection_of: ECO:0005521 ! S1 nuclease protection assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T16:22:58Z + +[Term] +id: ECO:0005667 +name: site-directed mutagenesis phenotypic evidence used in manual assertion +def: "A type of site-directed phenotypic mutagenesis evidence that is used in a manual assertion." [ECO:SN] +synonym: "site directed mutagenesis evidence" BROAD [] +synonym: "site-directed mutagenesis evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005528 ! site-directed mutagenesis phenotypic evidence +intersection_of: ECO:0005528 ! site-directed mutagenesis phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T16:29:13Z + +[Term] +id: ECO:0005668 +name: survival rate analysis evidence used in manual assertion +def: "A type of survival rate analysis evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001225 {is_inferred="true"} ! knockout evidence used in manual assertion +is_a: ECO:0001822 ! survival rate analysis evidence +intersection_of: ECO:0001822 ! survival rate analysis evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: snadendla +creation_date: 2016-05-09T16:31:08Z + +[Term] +id: ECO:0005669 +name: ultraviolet light footprinting evidence used in manual assertion +def: "A type of ultraviolet light footprinting evidence that is used in a manual assertion." [ECO:SN] +synonym: "UV footprinting" RELATED [] +is_a: ECO:0001826 ! ultraviolet light footprinting evidence +is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion +intersection_of: ECO:0001826 ! ultraviolet light footprinting evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: snadendla +creation_date: 2016-05-09T16:33:44Z + +[Term] +id: ECO:0005670 +name: x-ray crystallography evidence used in manual assertion +def: "A type of x-ray crystallography evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001171 {is_inferred="true"} ! crystallography evidence used in manual assertion +is_a: ECO:0001823 ! x-ray crystallography evidence +intersection_of: ECO:0001823 ! x-ray crystallography evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-10T11:53:00Z + +[Term] +id: ECO:0005671 +name: x-ray crystallography evidence used in automatic assertion +def: "A type of x-ray crystallography evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0001823 ! x-ray crystallography evidence +is_a: ECO:0007374 {is_inferred="true"} ! crystallography evidence used in automatic assertion +intersection_of: ECO:0001823 ! x-ray crystallography evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: snadendla +creation_date: 2016-05-10T11:54:03Z + +[Term] +id: ECO:0005672 +name: xylE reporter gene assay evidence used in manual assertion +def: "A type of xylE reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0005537 ! xylE reporter gene assay evidence +intersection_of: ECO:0005537 ! xylE reporter gene assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla +creation_date: 2016-05-10T11:57:15Z + +[Term] +id: ECO:0005673 +name: ad-hoc qualitative phenotype observation evidence +def: "A type of experimental phenotypic evidence where a non-standard trait (e.g. natural competence) is assessed qualitatively (e.g. presence/absence) and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor." [ECO:SN, PMID:15150239] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2016-05-10T12:57:41Z + +[Term] +id: ECO:0005674 +name: ad-hoc qualitative phenotype observation evidence used in manual assertion +def: "A type of ad-hoc qualitative phenotype observation that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005673 ! ad-hoc qualitative phenotype observation evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0005673 ! ad-hoc qualitative phenotype observation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-10T14:28:47Z + +[Term] +id: ECO:0005675 +name: ad-hoc quantitative phenotype observation evidence +def: "A type of experimental phenotypic evidence where a quantifiable phenotypic trait (e.g. glucose intake) is assessed in a quantitative manner after induction of the regulatory mechanism and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor." [ECO:SN, PMID:15150239] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: snadendla +creation_date: 2016-05-10T14:30:50Z + +[Term] +id: ECO:0005676 +name: ad-hoc quantitative phenotype observation evidence used in manual assertion +def: "A type of ad-hoc quantitative phenotype observation evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005675 ! ad-hoc quantitative phenotype observation evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0005675 ! ad-hoc quantitative phenotype observation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: snadendla +creation_date: 2016-05-10T14:34:50Z + +[Term] +id: ECO:0005800 +name: dilution assay evidence +def: "A type of direct assay evidence where the activity of a solution is determined by exposing an indicator culture or organism to a series of dilutions and determining the lowest concentration that elicits a biological response." [http://www.sciencedirect.com/science/article/pii/016770129400068I] +comment: For chemicals, bacteriocins, or phage, the indicator culture is typically present in a soft-agar layer on top of nutrient agar and a small quantity of each dilution is spotted onto the soft agar overlay. +synonym: "critical dilution assay" EXACT [] +synonym: "spot assay" EXACT [] +is_a: ECO:0000002 ! direct assay evidence + +[Term] +id: ECO:0005801 +name: enzymatic activity assay evidence used in manual assertion +def: "A type of enzyme assay evidence that is used in a manual assertion." [ECO:SN] +synonym: "enzyme assays" BROAD [] +is_a: ECO:0000005 ! enzymatic activity assay evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000005 ! enzymatic activity assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string + +[Term] +id: ECO:0005802 +name: cell transfection experiment evidence used in manual assertion +def: "A type of cell transfection experiment evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0005606 ! cell transfection experiment evidence +intersection_of: ECO:0005606 ! cell transfection experiment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string + +[Term] +id: ECO:0005803 +name: motility assay evidence +def: "A type of direct assay evidence where the motility of a cell or cells is determined." [ECO:SN] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000112 "A motility assay at 37 degrees C revealed a non-motile phenotype for all strains, as expected (data not shown)." xsd:string {xref="PMID:20830609"} + +[Term] +id: ECO:0005804 +name: immunofluorescence evidence used in manual assertion +def: "A type of immunofluorescence evidence that is used in a manual assertion." [ECO:SN] +synonym: "immunofluorescence" BROAD [] +is_a: ECO:0000007 ! immunofluorescence evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000007 ! immunofluorescence evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string + +[Term] +id: ECO:0005805 +name: yeast 2-hybrid evidence used in manual assertion +def: "A type of yeast 2-hybrid evidence that is used in a manual assertion." [ECO:SN] +synonym: "yeast two-hybrid assay" BROAD [] +is_a: ECO:0000068 ! yeast 2-hybrid evidence +is_a: ECO:0006077 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in manual assertion +intersection_of: ECO:0000068 ! yeast 2-hybrid evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string + +[Term] +id: ECO:0006001 +name: Cya fusion reporter assay evidence +def: "A type of evidence derived from exploiting the CyaA (calmodulin-activated adenylate cyclase toxin) secreted by Bordatella pertussis to synthesize cAMP and alter cellular physiology in a host cell for various biological applications." [PMID:10217833, PMID:14702323] +is_a: ECO:0000017 ! ectopic expression evidence +is_a: ECO:0000049 ! reporter gene assay evidence +created_by: rctauber +creation_date: 2016-06-15T08:42:16Z + +[Term] +id: ECO:0006002 +name: Cya fusion reporter assay evidence used in manual assertion +def: "A type of Cya fusion reporter assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0001273 {is_inferred="true"} ! ectopic expression evidence used in manual assertion +is_a: ECO:0006001 ! Cya fusion reporter assay evidence +intersection_of: ECO:0006001 ! Cya fusion reporter assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +property_value: IAO:0000234 "CollecTF" xsd:string +created_by: rctauber +creation_date: 2010-06-15T08:44:10Z + +[Term] +id: ECO:0006003 +name: electron microscopy evidence used in manual assertion +def: "A type of electron microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion +is_a: ECO:0005033 ! electron microscopy evidence +intersection_of: ECO:0005033 ! electron microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-10-12T12:32:29Z + +[Term] +id: ECO:0006004 +name: super-resolution microscopy evidence used in manual assertion +def: "A type of super-resolution microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion +is_a: ECO:0005578 ! super-resolution microscopy evidence +intersection_of: ECO:0005578 ! super-resolution microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-10-12T12:32:29Z + +[Term] +id: ECO:0006005 +name: fractionation evidence used in manual assertion +def: "A type of fractionation evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000100 ! fractionation evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000100 ! fractionation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-10-12T12:32:29Z + +[Term] +id: ECO:0006006 +name: electrophysiology assay evidence used in manual assertion +def: "A type of electrophysiology assay evidence that is used in a manual assertion." [] {comment="ECO:RCT"} +is_a: ECO:0000164 ! electrophysiology assay evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000164 ! electrophysiology assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-10-12T12:32:29Z + +[Term] +id: ECO:0006007 +name: chromatin immunoprecipitation-chip evidence used in manual assertion +def: "A type of chromatin immunoprecipitation-chip evidence that is used in a manual assertion." [ECO:RCT] +synonym: "ChIP-chip evidence" BROAD [] +synonym: "ChIP-on-chip evidence" BROAD [] +is_a: ECO:0000230 ! chromatin immunoprecipitation-chip evidence +is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion +intersection_of: ECO:0000230 ! chromatin immunoprecipitation-chip evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2016-10-21T11:55:53Z + +[Term] +id: ECO:0006008 +name: chromatin immunoprecipitation- exonuclease evidence used in manual assertion +def: "A type of chromatin immunoprecipitation- exonuclease evidence that is used in a manual assertion." [ECO:RCT] +synonym: "ChIP-exo" BROAD [] +is_a: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence +is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion +intersection_of: ECO:0001806 ! chromatin immunoprecipitation- exonuclease evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2016-10-21T11:55:53Z + +[Term] +id: ECO:0006009 +name: chromatin immunoprecipitation-seq evidence used in manual assertion +def: "A type of chromatin immunoprecipitation-seq evidence that is used in a manual assertion." [ECO:RCT] +synonym: "ChIP-SEQ evidence" BROAD [] +synonym: "ChIP-seq evidence" BROAD [] +is_a: ECO:0000229 ! chromatin immunoprecipitation-seq evidence +is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion +intersection_of: ECO:0000229 ! chromatin immunoprecipitation-seq evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2016-10-21T11:55:53Z + +[Term] +id: ECO:0006010 +name: mRNA interactome capture evidence +def: "A type of nucleic acid binding evidence in which RNA-binding proteins are identified through cross-linking RNA and proteins by UV light, capturing RNA-protein complexes on oligo(dT) beads, and finally identifying by mass spectrometry." [PMID:27729395] +synonym: "ultraviolet light cross-linking RNA binding evidence" EXACT [] +is_a: ECO:0000136 ! nucleic acid binding evidence +created_by: rctauber +creation_date: 2016-10-21T12:28:17Z + +[Term] +id: ECO:0006011 +name: mRNA interactome capture evidence used in manual assertion +def: "A type of mRNA interactome capture evidence that is used in a manual assertion." [ECO:RCT] +synonym: "ultraviolet light cross-linking RNA binding evidence" BROAD [] +is_a: ECO:0006010 ! mRNA interactome capture evidence +is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion +intersection_of: ECO:0006010 ! mRNA interactome capture evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2016-10-21T12:28:17Z + +[Term] +id: ECO:0006012 +name: patch-clamp recording evidence +def: "A type of electrophysiology assay evidence in which a glass micropipette is sealed to the surface of a cell membrane (patch) to study ion channels." [ECO:RCT] +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: rctauber +creation_date: 2016-11-28T11:44:05Z + +[Term] +id: ECO:0006013 +name: patch-clamp recording evidence used in manual assertion +def: "A type of patch-clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006006 ! electrophysiology assay evidence used in manual assertion +is_a: ECO:0006012 ! patch-clamp recording evidence +intersection_of: ECO:0006012 ! patch-clamp recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-11-28T11:44:05Z + +[Term] +id: ECO:0006014 +name: whole-cell patch-clamp recording evidence +def: "A type of patch-clamp recording evidence in which a glass micropipette filled with a prepared solution is used to form a seal with the cell membrane followed by rupture of membrane to provide accurate and high resolution electrical property measurements of the whole cell." [PMID:27341060] +is_a: ECO:0006012 ! patch-clamp recording evidence +created_by: rctauber +creation_date: 2016-11-28T11:44:05Z + +[Term] +id: ECO:0006015 +name: whole-cell patch-clamp recording evidence used in manual assertion +def: "A type of whole-cell patch-clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006013 ! patch-clamp recording evidence used in manual assertion +is_a: ECO:0006014 ! whole-cell patch-clamp recording evidence +intersection_of: ECO:0006014 ! whole-cell patch-clamp recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-11-28T11:44:05Z + +[Term] +id: ECO:0006016 +name: author statement from published clinical study +def: "A type of traceable author statement that is based on a publication about a clinical study." [] +synonym: "published clinical study evidence" EXACT [] +synonym: "traceable author statement from published clinical study" RELATED [] +is_a: ECO:0000033 ! author statement supported by traceable reference +created_by: rctauber +creation_date: 2016-11-30T09:44:47Z + +[Term] +id: ECO:0006017 +name: author statement from published clinical study used in manual assertion +def: "A type of author statement from published clinical study that is used in a manual assertion." [] +comment: Created and used by the Human Phenotype Ontology (HPO) Annotations group. Here it is used when evidence for an HPO annotation is information extracted from articles in the medical literature. +synonym: "PCS" EXACT [HPO:PCS] +synonym: "published clinical study evidence" BROAD [] +is_a: ECO:0000304 {is_inferred="true"} ! author statement supported by traceable reference used in manual assertion +is_a: ECO:0006016 ! author statement from published clinical study +intersection_of: ECO:0006016 ! author statement from published clinical study +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "TAS" xsd:string +property_value: seeAlso http://human-phenotype-ontology.github.io/documentation.html#annot xsd:string +created_by: rctauber +creation_date: 2016-11-30T09:44:47Z + +[Term] +id: ECO:0006018 +name: inference based on individual clinical experience +def: "A type of curator inference that is based on the individual clinical experience of a clinician" [] +synonym: "individual clinical experience evidence" EXACT [] +is_a: ECO:0000205 ! curator inference +created_by: rctauber +creation_date: 2016-11-30T09:44:47Z + +[Term] +id: ECO:0006019 +name: inference based on individual clinical experience used in manual assertion +def: "A type of inference based on individual clinical experience that is used in a manual assertion." [] +comment: Created and used by the Human Phenotype Ontology (HPO) Annotations group. Here it is used for annotating disorders with a limited amount of published data, and is accompanied by a reference to the individual or center performing the annotation. +synonym: "ICE" EXACT [HPO:ICE] +synonym: "individual clinical experience evidence" BROAD [] +is_a: ECO:0000305 {is_inferred="true"} ! curator inference used in manual assertion +is_a: ECO:0006018 ! inference based on individual clinical experience +intersection_of: ECO:0006018 ! inference based on individual clinical experience +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +property_value: seeAlso http://human-phenotype-ontology.github.io/documentation.html#annot xsd:string +created_by: rctauber +creation_date: 2016-11-30T09:44:47Z + +[Term] +id: ECO:0006020 +name: biofilm formation assay evidence +def: "A type of cell proliferation assay evidence in which biofilm growth is monitored and detected from attachment to development." [PMID:10547784] +synonym: "biofilm assay evidence" EXACT [] +is_a: ECO:0005007 ! cell proliferation assay evidence +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006021 +name: biofilm formation assay evidence used in manual assertion +def: "A type of biofilm formation assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0006020 ! biofilm formation assay evidence +intersection_of: ECO:0006020 ! biofilm formation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006022 +name: microtiter plate biofilm assay evidence +def: "A type of biofilm formation assay evidence in which microtiter dishes or tubes are inoculated and incubated to promote biofilm formation, and then biofilms are detected by staining with crystal violet or safranin to observe phenotypes." [PMID:10547784] +synonym: "96-well biofilm assay evidence" EXACT [] +is_a: ECO:0006020 ! biofilm formation assay evidence +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006023 +name: microtiter plate biofilm assay evidence used in manual assertion +def: "A type of microtiter plate biofilm assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006021 {is_inferred="true"} ! biofilm formation assay evidence used in manual assertion +is_a: ECO:0006022 ! microtiter plate biofilm assay evidence +intersection_of: ECO:0006022 ! microtiter plate biofilm assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006024 +name: air-liquid interface assay evidence +def: "A type of biofilm formation assay evidence in which biofilm formation is analyzed, without staining, over 4 to 48 hours by growth on a tilted multiwell plate." [PMID:18770545] +comment: Tilting of the plate positions the air-liquid interface on a clear portion to improve visability. +synonym: "ALI biofilm assay evidence" EXACT [] +is_a: ECO:0006020 ! biofilm formation assay evidence +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006025 +name: air-liquid interface assay evidence used in manual assertion +def: "A type of air-liquid interface assay evidence that is used in a manual assertion." [ECO:RCT] +synonym: "ALI biofilm assay evidence" BROAD [] +is_a: ECO:0006021 {is_inferred="true"} ! biofilm formation assay evidence used in manual assertion +is_a: ECO:0006024 ! air-liquid interface assay evidence +intersection_of: ECO:0006024 ! air-liquid interface assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006026 +name: colony biofilm assay evidence +def: "A type of biofilm formation assay evidence in which a colony is grown on a semipermeable membrane on an agar plate. The plate serves to supply nutrients and the semipermeable membrane can be relocated to fresh plates." [PMID:18770545] +comment: The plates commonly contain different carbon sources or antibiotic treatments to observe properties of the cells. +is_a: ECO:0006020 ! biofilm formation assay evidence +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006027 +name: colony biofilm assay evidence used in manual assertion +def: "A type of colony biofilm assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006021 {is_inferred="true"} ! biofilm formation assay evidence used in manual assertion +is_a: ECO:0006026 ! colony biofilm assay evidence +intersection_of: ECO:0006026 ! colony biofilm assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006028 +name: Kadouri drip-fed biofilm assay evidence +def: "A type of biofilm formation assay evidence in which mature bacterial biofilms are grown in a multiwell plate that has a growth medium continually pumped through the wells while waste is continually pumped out." [PMID:18770545] +is_a: ECO:0006020 ! biofilm formation assay evidence +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006029 +name: Kadouri drip-fed biofilm assay evidence used in manual assertion +def: "A type of Kadouri drip-fed biofilm assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006021 {is_inferred="true"} ! biofilm formation assay evidence used in manual assertion +is_a: ECO:0006028 ! Kadouri drip-fed biofilm assay evidence +intersection_of: ECO:0006028 ! Kadouri drip-fed biofilm assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2016-12-05T09:33:18Z + +[Term] +id: ECO:0006030 +name: co-immunoprecipitation evidence used in manual assertion +def: "A type of co-immunoprecipitation evidence that is used in a manual assertion." [ECO:RCT] +synonym: "co-immunoprecipitation" BROAD [] +is_a: ECO:0000070 ! co-immunoprecipitation evidence +is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion +intersection_of: ECO:0000070 ! co-immunoprecipitation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2016-01-13T11:32:26Z + +[Term] +id: ECO:0006031 +name: immunolocalization evidence used in manual assertion +def: "A type of immunolocalization evidence that is used in a manual assertion." [ECO:RCT] +synonym: "immunolocalization" BROAD [] +is_a: ECO:0000087 ! immunolocalization evidence +is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion +intersection_of: ECO:0000087 ! immunolocalization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-01-23T07:54:37Z + +[Term] +id: ECO:0006032 +name: optogenetic evidence +def: "A type of experimental phenotypic evidence arising from experiment in which neuronal activity is manipulated using genetically encoded, optically activated neuronal actuators." [GOC:DOS, PMID:17035522] +synonym: "optogenetic actuator evidence" EXACT [] +is_a: ECO:0000059 ! experimental phenotypic evidence +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006033 +name: optogenetic evidence used in manual assertion +def: "A type of optogenetic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "optogenetic actuator evidence" BROAD [] +is_a: ECO:0006032 ! optogenetic evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0006032 ! optogenetic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006034 +name: fluorescent sensor evidence +def: "A type of fluorescence evidence that is based on direct, quantitative measurement of some cellular property using a fluorescent sensor." [GOC:DOS] +comment: Examples include sensors for ion concentration and potential difference across a membrane. +is_a: ECO:0001115 ! fluorescence evidence +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006035 +name: fluorescent sensor evidence used in manual assertion +def: "A type of fluorescent sensor evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion +is_a: ECO:0006034 ! fluorescent sensor evidence +intersection_of: ECO:0006034 ! fluorescent sensor evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006036 +name: genetically encoded fluorescent sensor evidence +def: "A type of fluorescent sensor evidence that is based on direct, quantitative measurement of some cellular property using a genetically encoded fluorescent sensor." [GOC:DOS] +synonym: "optogenetic sensor evidence" EXACT [] +is_a: ECO:0006034 ! fluorescent sensor evidence +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006037 +name: genetically encoded fluorescent sensor evidence used in manual assertion +def: "A type of genetically encoded fluorescent sensor evidence that is used in a manual assertion." [ECO:RCT] +synonym: "optogenetic sensor evidence" BROAD [] +is_a: ECO:0006035 {is_inferred="true"} ! fluorescent sensor evidence used in manual assertion +is_a: ECO:0006036 ! genetically encoded fluorescent sensor evidence +intersection_of: ECO:0006036 ! genetically encoded fluorescent sensor evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006038 +name: genetically encoded fluorescent electrophysiology assay evidence +def: "A type of fluorescent sensor evidence and electrophysiology assay evidence where the electrical properties of cells or tissues are studied using fluorescent sensors." [GOC:DOS] +comment: Examples include genetically encoded sensors that detect potential difference across plasma membrane. +synonym: "electrophysiology - optical assay evidence" EXACT [] +is_a: ECO:0000164 ! electrophysiology assay evidence +is_a: ECO:0006034 ! fluorescent sensor evidence +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006039 +name: genetically encoded fluorescent electrophysiology assay evidence used in manual assertion +def: "A type of genetically encoded fluorescent electrophysiology assay evidence that is used in a manual assertion." [ECO:RCT] +synonym: "electrophysiology - optical assay evidence" BROAD [] +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +is_a: ECO:0006035 {is_inferred="true"} ! fluorescent sensor evidence used in manual assertion +is_a: ECO:0006038 ! genetically encoded fluorescent electrophysiology assay evidence +intersection_of: ECO:0006038 ! genetically encoded fluorescent electrophysiology assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006040 +name: genetically encoded fluorescent ion concentration sensor assay evidence +def: "A type of genetically encoded fluorescent electrophysiology assay evidence that is based on direct, quantitative measurement of ion concentration using a genetically encoded fluorescent sensor." [GOC:DOS] +comment: Examples include the genetically encoded calcium indicator GCaMP. +is_a: ECO:0006038 ! genetically encoded fluorescent electrophysiology assay evidence +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006041 +name: genetically encoded fluorescent ion concentration sensor assay evidence used in manual assertion +def: "A type of genetically encoded fluorescent ion concentration sensor assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006039 {is_inferred="true"} ! genetically encoded fluorescent electrophysiology assay evidence used in manual assertion +is_a: ECO:0006040 ! genetically encoded fluorescent ion concentration sensor assay evidence +intersection_of: ECO:0006040 ! genetically encoded fluorescent ion concentration sensor assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-01-23T11:58:48Z + +[Term] +id: ECO:0006042 +name: cell fractionation evidence used in manual assertion +def: "A type of cell fractionation evidence that is used in a manual assertion." [ECO:RCT] +synonym: "cell fractionation" BROAD [] +is_a: ECO:0000004 ! cell fractionation evidence +is_a: ECO:0006005 {is_inferred="true"} ! fractionation evidence used in manual assertion +intersection_of: ECO:0000004 ! cell fractionation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006043 +name: extracellular recording evidence +def: "A type of electrophysiology assay evidence resulting from the use of electrodes to measure in vivo electrical activity coming from adjacent neurons." [url:http\://www.nature.com/subjects/extracellular-recording] +is_a: ECO:0000164 ! electrophysiology assay evidence +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006044 +name: extracellular recording evidence used in manual assertion +def: "A type of extracellular recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +is_a: ECO:0006043 ! extracellular recording evidence +intersection_of: ECO:0006043 ! extracellular recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006045 +name: single-unit extracellular recording evidence +def: "A type of extracellular recording evidence in which an extracellular microelectrode is used to measure the electrical activity of a single neuron." [doi:10.1385/0-89603-185-3\:1] +is_a: ECO:0006043 ! extracellular recording evidence +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006046 +name: single-unit extracellular recording evidence used in manual assertion +def: "A type of single-unit extracellular recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006044 {is_inferred="true"} ! extracellular recording evidence used in manual assertion +is_a: ECO:0006045 ! single-unit extracellular recording evidence +intersection_of: ECO:0006045 ! single-unit extracellular recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006047 +name: field potential recording evidence +def: "A type of extracellular recording evidence in which electrical activity is measured in either tissue or at a cellular level with microelectrodes." [ECO:RCT] +synonym: "local field potential recording evidence" NARROW [] +is_a: ECO:0006043 ! extracellular recording evidence +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006048 +name: field potential recording evidence used in manual assertion +def: "A type of field potential recording evidence that is used in a manual assertion." [ECO:RCT] +synonym: "local field potential recording evidence" RELATED [] +is_a: ECO:0006044 {is_inferred="true"} ! extracellular recording evidence used in manual assertion +is_a: ECO:0006047 ! field potential recording evidence +intersection_of: ECO:0006047 ! field potential recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-02-21T07:26:28Z + +[Term] +id: ECO:0006049 +name: genetic transformation evidence used in manual assertion +def: "A type of genetic transformation evidence that is used in a manual assertion" [ECO:RCT] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005027 ! genetic transformation evidence +intersection_of: ECO:0005027 ! genetic transformation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2017-02-21T18:08:58Z + +[Term] +id: ECO:0006050 +name: anti-sense experiment evidence used in manual assertion +def: "A type of anti-sense experiment evidence that is used in a manual assertion." [ECO:RCT] +synonym: "anti-sense experiments" BROAD [] +is_a: ECO:0000018 ! anti-sense experiment evidence +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +intersection_of: ECO:0000018 ! anti-sense experiment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2017-02-21T18:08:58Z + +[Term] +id: ECO:0006051 +name: morpholino experiment evidence used in manual assertion +def: "A type of morpholino experiment evidence that is used in a manual assertion." [ECO:RCT] +synonym: "anti-sense evidence" BROAD [] +is_a: ECO:0000292 ! morpholino experiment evidence +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +intersection_of: ECO:0000292 ! morpholino experiment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2017-02-21T18:08:58Z + +[Term] +id: ECO:0006052 +name: RNAi evidence used in manual assertion +def: "A type of RNAi evidence that is used in a manual assertion." [ECO:RCT] +synonym: "RNAi experiment" BROAD [] +is_a: ECO:0000019 ! RNAi evidence +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +intersection_of: ECO:0000019 ! RNAi evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2017-02-21T18:08:58Z + +[Term] +id: ECO:0006053 +name: pharmacological assay evidence +def: "A type of experimental phenotypic evidence that arises from assaying the response of a cell, tissue, organ or organism following exposure to a receptor agonist or antagonist." [GOC:DOS] +is_a: ECO:0000059 ! experimental phenotypic evidence +created_by: dosumis +creation_date: 2017-03-07T08:51:02Z + +[Term] +id: ECO:0006054 +name: pharmacological assay evidence used in manual assertion +def: "A type of experimental phenotypic evidence used in a manual assertion that arises from assaying the response of a cell, tissue, organ or organism following exposure to a receptor agonist or antagonist." [GOC:DOS] +is_a: ECO:0006053 ! pharmacological assay evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0006053 ! pharmacological assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2017-03-07T08:51:02Z + +[Term] +id: ECO:0006055 +name: high throughput evidence +def: "A type of evidence where data generation is automated with equipment to allow for assaying samples or molecules in parallel." [ECO:MCC] +comment: Some relevant articles include: PMID: 23340846, doi:10.1038/nmeth0607-523 +synonym: "high throughput cell biology evidence" NARROW [] +synonym: "high throughput screening evidence" NARROW [] +synonym: "high-throughput evidence" EXACT [] +synonym: "HT evidence" EXACT [] +is_a: ECO:0000000 ! evidence +created_by: mchibucos +creation_date: 2017-03-28T14:48:39Z + +[Term] +id: ECO:0006056 +name: high throughput evidence used in manual assertion +def: "A type of evidence that is used in a manual assertion where data generation is automated with equipment to allow for assaying large numbers of samples or molecules in parallel." [ECO:RCT] +synonym: "high throughput cell biology evidence" RELATED [] +synonym: "high throughput screening evidence" RELATED [] +synonym: "high-throughput evidence" BROAD [] +synonym: "HT evidence" BROAD [] +synonym: "HTP" EXACT [GOECO:HTP] +synonym: "inferred from high throughput experiment" EXACT [GOECO:HTP] +xref: GOECO:HTP "inferred from high throughput experiment" +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0006055 ! high throughput evidence +intersection_of: ECO:0006055 ! high throughput evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "HTP" xsd:string {comment="Default"} +created_by: rctauber +creation_date: 2017-03-28T14:48:39Z + +[Term] +id: ECO:0006057 +name: high throughput evidence used in automatic assertion +def: "A type of evidence that is used in an automatic assertion where data generation is automated with equipment to allow for assaying samples or molecules in parallel." [ECO:RCT] +synonym: "high throughput cell biology evidence" RELATED [] +synonym: "high throughput screening evidence" RELATED [] +synonym: "high-throughput evidence" BROAD [] +synonym: "HT evidence" BROAD [] +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +is_a: ECO:0006055 ! high throughput evidence +intersection_of: ECO:0006055 ! high throughput evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2017-03-28T14:48:39Z + +[Term] +id: ECO:0006058 +name: high throughput cell biology evidence +def: "A type of high throughput evidence where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel." [ECO:MCC] +comment: HT methodologies may incorporate techniques from optics, chemistry, biology or image analysis (https://en.wikipedia.org/wiki/High_throughput_biology) +synonym: "omics experiment" RELATED [] +is_a: ECO:0006055 ! high throughput evidence +created_by: mchibucos +creation_date: 2017-03-28T14:48:39Z + +[Term] +id: ECO:0006059 +name: high throughput cell biology evidence used in manual assertion +def: "A type of high throughput evidence used in a manual assertion where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel." [ECO:RCT] +synonym: "omics experiment" RELATED [] +is_a: ECO:0006056 {is_inferred="true"} ! high throughput evidence used in manual assertion +is_a: ECO:0006058 ! high throughput cell biology evidence +intersection_of: ECO:0006058 ! high throughput cell biology evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "HTP" xsd:string +created_by: rctauber +creation_date: 2017-03-28T14:48:39Z + +[Term] +id: ECO:0006060 +name: high throughput cell biology evidence used in automatic assertion +def: "A type of high throughput evidence used in an automatic assertion where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel." [ECO:RCT] +synonym: "omics experiment" RELATED [] +is_a: ECO:0006057 {is_inferred="true"} ! high throughput evidence used in automatic assertion +is_a: ECO:0006058 ! high throughput cell biology evidence +intersection_of: ECO:0006058 ! high throughput cell biology evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2017-03-28T14:48:39Z + +[Term] +id: ECO:0006061 +name: immunofluorescence wide-field microscopy evidence used in manual assertion +def: "A type of wide-field fluorescence microscopy evidence used in a manual assertion where fluorescently tagged antibodies are imaged that are used to bind to their antigens." [ECO:SN, url:http\://journals.plos.org/plosone/article?id=10.1371/journal.pone.0057135] +synonym: "wide-field epifluorescence microscopy evidence" BROAD [] +is_a: ECO:0005599 ! immunofluorescence wide-field microscopy evidence +is_a: ECO:0005804 {is_inferred="true"} ! immunofluorescence evidence used in manual assertion +is_a: ECO:0006062 {is_inferred="true"} ! wide-field fluorescence microscopy evidence used in manual assertion +intersection_of: ECO:0005599 ! immunofluorescence wide-field microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-05-15T08:57:07Z + +[Term] +id: ECO:0006062 +name: wide-field fluorescence microscopy evidence used in manual assertion +def: "A type of wide-field microscopy evidence that is used in a manual assertion where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter." [ECO:SN, url:http\://www.bristol.ac.uk/synaptic/research/techniques/widefield.html] +is_a: ECO:0005590 {is_inferred="true"} ! wide-field microscopy evidence used in manual assertion +is_a: ECO:0005598 ! wide-field fluorescence microscopy evidence +intersection_of: ECO:0005598 ! wide-field fluorescence microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-05-15T09:01:19Z + +[Term] +id: ECO:0006063 +name: over expression analysis evidence used in manual assertion +def: "A type of experimental phenotypic evidence that is used in a manual assertion where a gene and/or gene product is investigated in a transgenic organism that has been engineered to overexpress that gene product." [PMID:22419077] +synonym: "analysis of overexpression/ectopic expression phenotype" RELATED [] +is_a: ECO:0000120 ! over expression analysis evidence +is_a: ECO:0006049 {is_inferred="true"} ! genetic transformation evidence used in manual assertion +intersection_of: ECO:0000120 ! over expression analysis evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2017-05-15T09:03:49Z + +[Term] +id: ECO:0006064 +name: cell-free assay evidence used in manual assertion +def: "A type of cell-free assay evidence that is used in a manual assertion." [PMID:18453125] +synonym: "in vitro assay evidence" BROAD [] +is_a: ECO:0000183 ! cell-free assay evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000183 ! cell-free assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-05-15T09:08:17Z + +[Term] +id: ECO:0006065 +name: in vitro cell based assay evidence used in manual assertion +comment: Use children of ECO:0001565 (cell-based assay evidence) in place of this term. +is_obsolete: true +created_by: rctauber +creation_date: 2017-05-15T09:11:13Z + +[Term] +id: ECO:0006066 +name: fluorescence recovery after photobleaching evidence +def: "A type of fluorescence evidence where quantitative information on the diffusion properties of a sample is produced from the measurement of diffusion of fluorescent probes over time into an area that has been photobleached by a high-intensity laser pulse." [PMID:26314367] +comment: FRAP is a fluorescence microscopy-based technique. {xref="PMID:26314367"} +synonym: "FRAP evidence" EXACT [] +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000234 "dosumis" xsd:string +created_by: rctauber +creation_date: 2017-05-16T10:55:40Z + +[Term] +id: ECO:0006067 +name: fluorescence recovery after photobleaching evidence used in manual assertion +def: "A type of fluorescence evidence that is used in a manual assertion where quantitative information on the diffusion properties of a sample is produced from the measurement of diffusion of fluorescent probes over time into an area that has been photobleached by a high-intensity laser pulse." [] +synonym: "FRAP evidence" BROAD [] +is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion +is_a: ECO:0006066 ! fluorescence recovery after photobleaching evidence +intersection_of: ECO:0006066 ! fluorescence recovery after photobleaching evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2017-05-16T10:55:40Z + +[Term] +id: ECO:0006068 +name: RNA-sequencing evidence used in manual assertion +def: "A type of high throughput nucleotide sequencing assay evidence used in a manual assertion based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC] +synonym: "RNA sequencing|differential gene expression evidence from RNA-seq experiment" RELATED [] +synonym: "RNA-seq evidence used in manual assertion" EXACT [] +synonym: "RNAseq evidence used in manual assertion" EXACT [] +synonym: "whole transcriptome shotgun sequencing" BROAD [] +synonym: "WTSS evidence" BROAD [] +is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion +is_a: ECO:0000295 ! RNA-sequencing evidence +intersection_of: ECO:0000295 ! RNA-sequencing evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2017-06-28T10:37:02Z + +[Term] +id: ECO:0006069 +name: RNA-sequencing evidence used in automatic assertion +def: "A type of high throughput nucleotide sequencing assay evidence used in an automatic assertion based on high-throughput (HT) sequencing of fragmented cDNA molecules." [ECO:MCC] +synonym: "RNA sequencing|differential gene expression evidence from RNA-seq experiment" RELATED [] +synonym: "RNA-seq evidence used in automatic assertion" EXACT [] +synonym: "RNAseq evidence used in automatic assertion" EXACT [] +synonym: "whole transcriptome shotgun sequencing" BROAD [] +synonym: "WTSS evidence" BROAD [] +is_a: ECO:0000295 ! RNA-sequencing evidence +is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion +intersection_of: ECO:0000295 ! RNA-sequencing evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2017-06-28T10:37:02Z + +[Term] +id: ECO:0006070 +name: immuno-labelling electron microscopy evidence +def: "A type of electron microscopy evidence resulting from the use of antibodies to identify the localization of antigens in cells and tissues." [PMID:25151300] +synonym: "immunolabelling electron microscopy evidence" EXACT [] +is_a: ECO:0005033 ! electron microscopy evidence +property_value: IAO:0000234 "dosumis" xsd:string +created_by: rctauber +creation_date: 2017-07-05T09:59:10Z + +[Term] +id: ECO:0006071 +name: immuno-labelling electron microscopy evidence used in manual assertion +def: "A type of electron microscopy evidence used in a manual assertion resulting from the use of antibodies to identify the localization of antigens in cells and tissues." [PMID:25151300] +synonym: "immunolabelling electron microscopy evidence" BROAD [] +is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion +is_a: ECO:0006070 ! immuno-labelling electron microscopy evidence +intersection_of: ECO:0006070 ! immuno-labelling electron microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000119 PMID:25151300 xsd:string +property_value: IAO:0000234 "dosumis" xsd:string +created_by: rctauber +creation_date: 2017-07-05T09:59:10Z + +[Term] +id: ECO:0006072 +name: immunofluorescence super resolution microscopy evidence +def: "A type of super-resolution microscopy evidence resulting from the use of fluorescently-tagged antibodies to identify the localization of antigens in cells and tissue." [PMID:19245833] +is_a: ECO:0005578 ! super-resolution microscopy evidence +property_value: IAO:0000234 "dosumis" xsd:string +created_by: rctauber +creation_date: 2017-07-05T09:59:10Z + +[Term] +id: ECO:0006073 +name: immunofluorescence super resolution microscopy evidence used in manual assertion +def: "A type of super-resolution microscopy evidence used in a manual assertion resulting from the use of fluorescently-tagged antibodies to identify the localization of antigens in cells and tissue." [PMID:19245833] +is_a: ECO:0006004 {is_inferred="true"} ! super-resolution microscopy evidence used in manual assertion +is_a: ECO:0006072 ! immunofluorescence super resolution microscopy evidence +intersection_of: ECO:0006072 ! immunofluorescence super resolution microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "dosumis" xsd:string +created_by: rctauber +creation_date: 2017-07-05T09:59:10Z + +[Term] +id: ECO:0006074 +name: co-purification evidence used in manual assertion +def: "A type of physical interaction evidence that is used in manual assertion where a cellular component subunit is isolated as part of purification of its larger complex." [TAIR:TED] +synonym: "co-purification" BROAD [] +is_a: ECO:0000022 ! co-purification evidence +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +intersection_of: ECO:0000022 ! co-purification evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:19Z + +[Term] +id: ECO:0006075 +name: affinity evidence used in manual assertion +def: "A type of physical interaction evidence that is used in manual assertion that depends on the strength of the interaction between two entities." [ECO:MCC] +synonym: "ligand binding evidence" RELATED [] +is_a: ECO:0000023 ! affinity evidence +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +intersection_of: ECO:0000023 ! affinity evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:20Z + +[Term] +id: ECO:0006076 +name: protein binding evidence used in manual assertion +def: "A type of affinity evidence that is used in manual assertion resulting from the binding of a molecule to a protein or protein complex." [GO:0005515] +is_a: ECO:0000024 ! protein binding evidence +is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion +intersection_of: ECO:0000024 ! protein binding evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:21Z + +[Term] +id: ECO:0006077 +name: bait-prey hybrid interaction evidence used in manual assertion +def: "A type of bait-prey hybrid interaction evidence that is used in manual assertion where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein." [ECO:MCC] +synonym: "bait-prey protein pull-down evidence" EXACT [] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence +is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion +intersection_of: ECO:0000025 ! bait-prey hybrid interaction evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:22Z + +[Term] +id: ECO:0006078 +name: immunological assay evidence used in manual assertion +def: "A type of affinity evidence that is used in manual assertion resulting from quantitation of the analyte which depends on the reaction of an antigen (analyte) and an antibody." [ERO:0001362] +is_a: ECO:0000040 ! immunological assay evidence +is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion +intersection_of: ECO:0000040 ! immunological assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:23Z + +[Term] +id: ECO:0006079 +name: yeast one-hybrid evidence used in manual assertion +def: "A type of hybrid interaction evidence that is used in manual assertion that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors." [ECO:MCC] +synonym: "yeast one-hybrid assay" BROAD [] +is_a: ECO:0000066 ! yeast one-hybrid evidence +is_a: ECO:0006077 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in manual assertion +intersection_of: ECO:0000066 ! yeast one-hybrid evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:24Z + +[Term] +id: ECO:0006080 +name: split-ubiquitin functional complementation evidence used in manual assertion +def: "A type of split-ubiquitin functional complementation evidence that is used in a manual assertion that is based on detection of protein-protein interaction between a bait and prey protein by in vivo reconstitution of split-ubiquitin (when bait and prey interact) and release of a reporter protein." [PMID:15064465] +synonym: "split-ubiquitin assay" BROAD [] +is_a: ECO:0000074 ! split-ubiquitin functional complementation evidence +is_a: ECO:0006293 {is_inferred="true"} ! protein fragment functional complementation evidence used in manual assertion +intersection_of: ECO:0000074 ! split-ubiquitin functional complementation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:25Z + +[Term] +id: ECO:0006081 +name: far-Western blotting evidence used in manual assertion +def: "A type of physical interaction evidence that is used in a manual assertion that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes." [PMID:18079728] +synonym: "far-Western analysis" BROAD [] +is_a: ECO:0000076 ! far-Western blotting evidence +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +intersection_of: ECO:0000076 ! far-Western blotting evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:26Z + +[Term] +id: ECO:0006082 +name: affinity chromatography evidence used in manual assertion +def: "A type of affinity evidence that is used in a manual assertion that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bond compound." [ECO:MCC] +synonym: "affinity chromatography" BROAD [] +is_a: ECO:0000079 ! affinity chromatography evidence +is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion +intersection_of: ECO:0000079 ! affinity chromatography evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:27Z + +[Term] +id: ECO:0006083 +name: nucleic acid binding evidence used in manual assertion +def: "A type of affinity evidence that is used in a manual assertion resulting from the binding of a molecule to a nucleic acid." [GO:0003676] +is_a: ECO:0000136 ! nucleic acid binding evidence +is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion +intersection_of: ECO:0000136 ! nucleic acid binding evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:28Z + +[Term] +id: ECO:0006084 +name: ribohomopolymer binding assay evidence used in manual assertion +def: "A type of nucleic acid binding evidence that is used in a manual assertion resulting from an enzyme displaying binding activity to specific ribohomopolymer." [PMC:102612] +synonym: "ribohomopolymer binding assay" BROAD [] +is_a: ECO:0000138 ! ribohomopolymer binding assay evidence +is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion +intersection_of: ECO:0000138 ! ribohomopolymer binding assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:29Z + +[Term] +id: ECO:0006085 +name: protein:ion binding evidence used in manual assertion +def: "A type of protein binding evidence that is used in a manual assertion resulting from a metal ion binding to a protein at a specific binding site." [PMID:2377604] +is_a: ECO:0000142 ! protein:ion binding evidence +is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion +intersection_of: ECO:0000142 ! protein:ion binding evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:30Z + +[Term] +id: ECO:0006086 +name: Southwestern blot evidence used in manual assertion +def: "A type of nucleic acid binding evidence that is used in a manual assertion in which DNA-protein binding is detected using labeled DNA as probes, hybridized to electrophoretically separated proteins." [ECO:RCT] +synonym: "Southwestern analysis" BROAD [] +is_a: ECO:0000144 ! Southwestern blot evidence +is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion +intersection_of: ECO:0000144 ! Southwestern blot evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:31Z + +[Term] +id: ECO:0006087 +name: Northwestern blot evidence used in manual assertion +def: "A type of nucleic acid binding evidence that is used in a manual assertion in which RNA-protein binding is detected using labeled RNA as probes, hybridized to electrophoretically separated proteins." [ECO:RCT] +synonym: "Northwestern analysis" BROAD [] +is_a: ECO:0000146 ! Northwestern blot evidence +is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion +intersection_of: ECO:0000146 ! Northwestern blot evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:32Z + +[Term] +id: ECO:0006088 +name: systematic evolution of ligands by exponential amplification evidence used in manual assertion +def: "A type of evidence that is used in a manual assertion arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand." [ECO:MCC] +synonym: "in vitro evolution evidence" RELATED [] +synonym: "in vitro selection evidence" BROAD [] +synonym: "SELEX evidence" BROAD [] +is_a: ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +intersection_of: ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:33Z + +[Term] +id: ECO:0006089 +name: bacterial one-hybrid evidence used in manual assertion +def: "A type of hybrid interaction evidence that is used in a manual assertion that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey)." [ECO:MCC] +synonym: "B1H evidence" BROAD [] +is_a: ECO:0000294 ! bacterial one-hybrid evidence +is_a: ECO:0006077 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in manual assertion +intersection_of: ECO:0000294 ! bacterial one-hybrid evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:34Z + +[Term] +id: ECO:0006090 +name: protein-oligonucleotide microarray binding evidence used in manual assertion +def: "A type of protein-oligonucleotide microarray binding evidence that is used in a manual assertion that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites." [PMID:22146299] +synonym: "PBM evidence" BROAD [] +is_a: ECO:0001601 ! protein-oligonucleotide microarray binding evidence +is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion +is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion +intersection_of: ECO:0001601 ! protein-oligonucleotide microarray binding evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:35Z + +[Term] +id: ECO:0006091 +name: functional complementation evidence used in manual assertion +def: "A type of genetic interaction evidence that is used in a manual assertion where a wild-type copy of the gene in question is inserted into a mutant cell to see if it restores the wild-type phenotype in the mutant background." [PMID:27403640] +synonym: "functional complementation" BROAD [] +is_a: ECO:0000012 ! functional complementation evidence +is_a: ECO:0000316 {is_inferred="true"} ! genetic interaction evidence used in manual assertion +intersection_of: ECO:0000012 ! functional complementation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:36Z + +[Term] +id: ECO:0006092 +name: transgenic rescue experiment evidence used in manual assertion +def: "A type of functional complementation evidence that is used in a manual assertion that is used in manual assertion resulting from the introduction of a transgene to prevent, or \"rescue\" an organism from a condition." [url:http\://www.nature.com/gt/journal/v11/n15/full/3302282a.html] +is_a: ECO:0000013 ! transgenic rescue experiment evidence +is_a: ECO:0006091 {is_inferred="true"} ! functional complementation evidence used in manual assertion +intersection_of: ECO:0000013 ! transgenic rescue experiment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:37Z + +[Term] +id: ECO:0006093 +name: transient rescue experiment evidence used in manual assertion +def: "A type of transient rescue experiment evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000038 ! transient rescue experiment evidence +is_a: ECO:0006091 {is_inferred="true"} ! functional complementation evidence used in manual assertion +intersection_of: ECO:0000038 ! transient rescue experiment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:38Z + +[Term] +id: ECO:0006094 +name: suppressor/enhancer interaction phenotypic evidence used in manual assertion +def: "A type of suppressor/enhancer interaction phenotypic evidence that is used in a manual assertion." [url:http\://www.wormbook.org/chapters/www\:geneticsuppression/geneticsuppression.html] +synonym: "'traditional' genetic interactions (e.g. suppressors, synthetic lethals)" BROAD [] +synonym: "suppressor/enhancer interaction evidence used in manual assertion" EXACT [] +is_a: ECO:0000052 ! suppressor/enhancer interaction phenotypic evidence +is_a: ECO:0000316 {is_inferred="true"} ! genetic interaction evidence used in manual assertion +intersection_of: ECO:0000052 ! suppressor/enhancer interaction phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:39Z + +[Term] +id: ECO:0006095 +name: double mutant phenotypic evidence used in manual assertion +def: "A type of double mutant phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "double mutant analysis" BROAD [] +synonym: "double mutant phenotype evidence used in manual assertion" EXACT [] +is_a: ECO:0000054 ! double mutant phenotypic evidence +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +intersection_of: ECO:0000054 ! double mutant phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:40Z + +[Term] +id: ECO:0006096 +name: epistatic interaction phenotypic evidence used in manual assertion +def: "A type of epistatic interaction phenotypic evidence that is used in a manual assertion." [PMID:18852697] +synonym: "epistatic interaction evidence used in manual assertion" EXACT [] +synonym: "epistatic interactions" BROAD [] +is_a: ECO:0000056 ! epistatic interaction phenotypic evidence +is_a: ECO:0000316 {is_inferred="true"} ! genetic interaction evidence used in manual assertion +intersection_of: ECO:0000056 ! epistatic interaction phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:41Z + +[Term] +id: ECO:0006097 +name: functional complementation in heterologous system evidence used in manual assertion +def: "A type of functional complementation evidence that is used in a manual assertion that is based on the insertion of a wild-type copy of a gene into a heterologous organism, with the mutation occurring in a homologous gene." [TAIR:TED] +synonym: "functional complementation in heterologous system" BROAD [] +is_a: ECO:0000064 ! functional complementation in heterologous system evidence +is_a: ECO:0006091 {is_inferred="true"} ! functional complementation evidence used in manual assertion +intersection_of: ECO:0000064 ! functional complementation in heterologous system evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IGI" xsd:string +created_by: rctauber +creation_date: 2017-09-14T09:19:42Z + +[Term] +id: ECO:0006098 +name: temperature-sensitive mutant phenotypic evidence +def: "A type of mutant phenotype evidence resulting from altered gene function at higher temperatures." [GOC:PG, PMID:19596904] +synonym: "temperature-sensitive mutant phenotype evidence" EXACT [] +synonym: "Ts mutation evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000234 "pgaudet" xsd:string +created_by: rctauber +creation_date: 2017-09-18T07:58:28Z + +[Term] +id: ECO:0006099 +name: temperature-sensitive mutant phenotypic evidence used in manual assertion +def: "A type of temperature-sensitive mutant phenotypic evidence that is used in a manual assertion." [GOC:PG, PMID:19596904] +synonym: "temperature-sensitive mutant phenotype evidence used in manual assertion" EXACT [] +synonym: "Ts mutation evidence" BROAD [] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0006098 ! temperature-sensitive mutant phenotypic evidence +intersection_of: ECO:0006098 ! temperature-sensitive mutant phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 "pgaudet" xsd:string +created_by: rctauber +creation_date: 2017-09-18T07:58:28Z + +[Term] +id: ECO:0006100 +name: recessive mutant phenotype evidence +def: "A type of mutant phenotype evidence based on the analysis of a mutation which, in diploid organisms, must be present in both alleles for a phenotype to manifest itself." [GOC:PG, NBK:21578] +is_a: ECO:0000015 ! mutant phenotype evidence +property_value: IAO:0000234 "pgaudet" xsd:string +created_by: rctauber +creation_date: 2017-09-18T07:58:28Z + +[Term] +id: ECO:0006101 +name: recessive mutant phenotype evidence used in manual assertion +def: "A type of mutant phenotype evidence that is used in a manual assertion based on the analysis of a mutation which, in diploid organisms, must be present in both alleles for a phenotype to manifest itself." [GOC:PG, NBK:21578] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0006100 ! recessive mutant phenotype evidence +intersection_of: ECO:0006100 ! recessive mutant phenotype evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +property_value: IAO:0000234 "pgaudet" xsd:string +created_by: rctauber +creation_date: 2017-09-18T07:58:28Z + +[Term] +id: ECO:0006135 +name: quantum mechanics/molecular mechanics simulation evidence +def: "A type of computational evidence where the active site of the molecular system is described with highly accurate quantum theory, while the contribution of the rest of the system is described with molecular mechanical force field." [ECO:SN, PMID:26930454] +synonym: "QM/MM evidence" EXACT [] +is_a: ECO:0007672 ! computational evidence +created_by: snadendla + +[Term] +id: ECO:0006136 +name: quantum mechanics/molecular mechanics simulation evidence used in automatic assertion +def: "A type of quantum mechanics/molecular mechanics simulation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006135 ! quantum mechanics/molecular mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006137 +name: quantum mechanics/molecular mechanics simulation evidence used in manual assertion +def: "A type of quantum mechanics/molecular mechanics simulation evidence that is used in manual assertion." [ECO:SN] +is_a: ECO:0006135 ! quantum mechanics/molecular mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006138 +name: molecular mechanics simulation evidence +def: "A type of computational evidence where the energy of a molecular system is predicted as a function of its conformation." [ECO:SN, url:http\://vergil.chemistry.gatech.edu/courses/chem6485/pdf/molmech.pdf] +synonym: "MM evidence" EXACT [] +is_a: ECO:0007672 ! computational evidence +created_by: snadendla + +[Term] +id: ECO:0006139 +name: molecular mechanics simulation evidence used in automatic assertion +def: "A type of molecular mechanics simulation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006138 ! molecular mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006140 +name: molecular mechanics simulation evidence used in manual assertion +def: "A type of molecular mechanics simulation evidence that is used in manual assertion." [ECO:SN] +is_a: ECO:0006138 ! molecular mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006141 +name: quantum mechanics simulation evidence +def: "A type of computational evidence where the behavior of matter and light on the atomic and subatomic scale is described." [ECO:SN, url:https\://www.britannica.com/science/quantum-mechanics-physics] +synonym: "QM evidence" EXACT [] +synonym: "quantum physics evidence" EXACT [] +synonym: "quantum theory evidence" EXACT [] +is_a: ECO:0007672 ! computational evidence +created_by: snadendla + +[Term] +id: ECO:0006142 +name: quantum mechanics simulation evidence used in automatic assertion +def: "A type of quantum mechanics simulation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006141 ! quantum mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006143 +name: quantum mechanics simulation evidence used in manual assertion +def: "A type of quantum mechanics simulation evidence that is used in manual assertion." [ECO:SN] +is_a: ECO:0006141 ! quantum mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006144 +name: density functional theory simulation evidence +def: "A type of quantum mechanics simulation evidence that results from the calculation of ground-state electronic structure of atoms, molecules and solid state materials." [ECO:SN, url:https\://www.sciencedirect.com/topics/physics-and-astronomy/density-functional-theory] +synonym: "DFT evidence" EXACT [] +is_a: ECO:0006141 ! quantum mechanics simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006145 +name: density functional theory simulation evidence used in manual assertion +def: "A type of density functional theory simulation evidence that is used in manual assertion." [ECO:SN] +is_a: ECO:0006144 ! density functional theory simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006146 +name: density functional theory simulation evidence used in automatic assertion +def: "A type of density functional theory simulation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006144 ! density functional theory simulation evidence +created_by: snadendla + +[Term] +id: ECO:0006151 +name: documented statement evidence +def: "A type of evidence in which information is recorded in some documentation system, for example, but not limited to a publication, survey, or medical record." [ECO:SN] +is_a: ECO:0000000 ! evidence +created_by: snadendla + +[Term] +id: ECO:0006152 +name: medical practitioner statement evidence +def: "A type of documented statement evidence in which information is stated by a health care professional, for example, but not limited to a doctor, nurse, or psychologist." [ECO:SN] +is_a: ECO:0006151 ! documented statement evidence +created_by: snadendla + +[Term] +id: ECO:0006153 +name: self-reported individual's statement evidence +def: "A type of documented statement evidence in which information is provided by an individual through means including, but not limited to, paper form, electronic form, or verbal communication, and is captured in a documented record." [ECO:SN] +is_a: ECO:0006151 ! documented statement evidence +created_by: snadendla + +[Term] +id: ECO:0006154 +name: self-reported patient statement evidence +def: "A type of self-reported individual's statement evidence in which information is provided by a patient in a clinical setting." [ECO:SN] +is_a: ECO:0006153 ! self-reported individual's statement evidence +property_value: IAO:0000234 "Pablo Botas for Foundation 29, Dx29 project" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006155 +name: documented statement evidence used in manual assertion +def: "A type of documented statement evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0006151 ! documented statement evidence +intersection_of: ECO:0006151 ! documented statement evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: snadendla + +[Term] +id: ECO:0006156 +name: documented statement evidence used in automatic assertion +def: "A type of documented statement evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +is_a: ECO:0006151 ! documented statement evidence +intersection_of: ECO:0006151 ! documented statement evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006157 +name: self-reported individual's statement evidence used in manual assertion +def: "A type of self-reported individual's statement evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006153 ! self-reported individual's statement evidence +is_a: ECO:0006155 {is_inferred="true"} ! documented statement evidence used in manual assertion +intersection_of: ECO:0006153 ! self-reported individual's statement evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: snadendla + +[Term] +id: ECO:0006158 +name: self-reported individual's statement evidence used in automatic assertion +def: "A type of self-reported individual's statement evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006153 ! self-reported individual's statement evidence +is_a: ECO:0006156 {is_inferred="true"} ! documented statement evidence used in automatic assertion +intersection_of: ECO:0006153 ! self-reported individual's statement evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006159 +name: self-reported patient statement evidence used in manual assertion +def: "A type of self-reported patient statement evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006154 ! self-reported patient statement evidence +is_a: ECO:0006157 {is_inferred="true"} ! self-reported individual's statement evidence used in manual assertion +intersection_of: ECO:0006154 ! self-reported patient statement evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: snadendla + +[Term] +id: ECO:0006160 +name: self-reported patient statement evidence used in automatic assertion +def: "A type of self-reported patient statement evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006154 ! self-reported patient statement evidence +is_a: ECO:0006158 {is_inferred="true"} ! self-reported individual's statement evidence used in automatic assertion +intersection_of: ECO:0006154 ! self-reported patient statement evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006161 +name: medical practitioner statement evidence used in manual assertion +def: "A type of medical practitioner statement evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006152 ! medical practitioner statement evidence +is_a: ECO:0006155 {is_inferred="true"} ! documented statement evidence used in manual assertion +intersection_of: ECO:0006152 ! medical practitioner statement evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: snadendla + +[Term] +id: ECO:0006162 +name: medical practitioner statement evidence used in automatic assertion +def: "A type of medical practitioner statement evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006152 ! medical practitioner statement evidence +is_a: ECO:0006156 {is_inferred="true"} ! documented statement evidence used in automatic assertion +intersection_of: ECO:0006152 ! medical practitioner statement evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006163 +name: nuclear magnetic resonance spectroscopy evidence +def: "A type of nuclear magnetic resonance evidence used for quantification of metabolites or for the determination of chemical structure or composition." [ECO:SN, PMID: 16428685, PMID: 23036848] +synonym: "NMR Spectroscopy" EXACT [] +is_a: ECO:0001105 ! nuclear magnetic resonance evidence +relationship: OBI:0000312 OBI:0000623 +property_value: IAO:0000234 SIB:PG xsd:string +created_by: snadendla + +[Term] +id: ECO:0006164 +name: nuclear magnetic resonance spectroscopy evidence used in automatic assertion +def: "A type of nuclear magnetic resonance spectroscopy evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence +is_a: ECO:0007441 {is_inferred="true"} ! nuclear magnetic resonance evidence used in automatic assertion +intersection_of: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 SIB:PG xsd:string +created_by: snadendla + +[Term] +id: ECO:0006165 +name: nuclear magnetic resonance spectroscopy evidence used in manual assertion +def: "A type of nuclear magnetic resonance spectroscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "NMR spectroscopy evidence" BROAD [] +is_a: ECO:0001238 {is_inferred="true"} ! nuclear magnetic resonance evidence used in manual assertion +is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence +intersection_of: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 SIB:PG xsd:string +created_by: snadendla + +[Term] +id: ECO:0006166 +name: nuclear magnetic resonance imaging evidence +def: "A type of nuclear magnetic resonance evidence used to image anatomy and physiological processes." [url:https\://en.wikipedia.org/wiki/Magnetic_resonance_imaging] +is_a: ECO:0001105 ! nuclear magnetic resonance evidence +property_value: IAO:0000234 "bjonnh" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006167 +name: nuclear magnetic resonance imaging evidence used in automatic assertion +def: "A type of nuclear magnetic resonance imaging evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006166 ! nuclear magnetic resonance imaging evidence +is_a: ECO:0007441 {is_inferred="true"} ! nuclear magnetic resonance evidence used in automatic assertion +intersection_of: ECO:0006166 ! nuclear magnetic resonance imaging evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 "bjonnh" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006168 +name: nuclear magnetic resonance imaging evidence used in manual assertion +def: "A type of nuclear magnetic resonance imaging evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001238 {is_inferred="true"} ! nuclear magnetic resonance evidence used in manual assertion +is_a: ECO:0006166 ! nuclear magnetic resonance imaging evidence +intersection_of: ECO:0006166 ! nuclear magnetic resonance imaging evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "bjonnh" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006169 +name: quantitative western immunoblotting evidence +def: "A type of qualitative western immunoblotting evidence where detection of the signal is carried out through digital imaging and the signal is normalized with methods such as standard curves built from reference housekeeping proteins or total transferred protein measurements." [ECO:SN, PMID:25852189] +is_a: ECO:0000112 ! qualitative western immunoblotting evidence +property_value: IAO:0000234 GO:Val xsd:string +created_by: snadendla + +[Term] +id: ECO:0006170 +name: quantitative western immunoblotting evidence used in manual evidence +def: "A type of quantitative western immunoblotting evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000279 {is_inferred="true"} ! qualitative western immunoblotting evidence used in manual assertion +is_a: ECO:0006169 ! quantitative western immunoblotting evidence +intersection_of: ECO:0006169 ! quantitative western immunoblotting evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006171 +name: quantitative western immunoblotting evidence used in automatic assertion +def: "A type of quantitatative western immunoblotting evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006169 ! quantitative western immunoblotting evidence +is_a: ECO:0007306 {is_inferred="true"} ! qualitative western immunoblotting evidence used in automatic assertion +intersection_of: ECO:0006169 ! quantitative western immunoblotting evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: snadendla + +[Term] +id: ECO:0006172 +name: mixed support of intron positions by RNA-sequencing alignment evidence +def: "A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignments from a mixture of samples support all of the intron positions (exon pairs) predicted for a transcript." [ECO:SN] +comment: Mixed support entails that all exon pairs represented in the transcript are supported, but require a combination of evidence from multiple samples. For example, for a transcript containing five exons, if liver RNA-seq reads support the first two introns and brain RNA-seq supports the last three introns, then neither sample meets the criteria for full support (ECO:0000343) but the combination of samples qualifies as Mixed support. Mixed support may result from differential expression, or low expression levels that benefit from combining data. +synonym: "mixed support of intron positions by RNA-seq alignment evidence" EXACT [] +is_a: ECO:0000342 ! support of intron positions by RNA-sequencing alignment evidence +property_value: IAO:0000234 "NCBI-RefSeq" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006173 +name: mixed support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +def: "A type of mixed support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion." [ECO:SN] +synonym: "mixed support of intron positions by RNA-seq alignment evidence used in automatic assertion" EXACT [] +is_a: ECO:0000347 {is_inferred="true"} ! support of intron positions by RNA-sequencing alignment evidence used in automatic assertion +is_a: ECO:0006172 ! mixed support of intron positions by RNA-sequencing alignment evidence +intersection_of: ECO:0006172 ! mixed support of intron positions by RNA-sequencing alignment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 "NCBI-RefSeq" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006174 +name: mixed support of intron positions by RNA-sequencing alignment evidence used in manual assertion +def: "A type of mixed support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion" [ECO:SN] +synonym: "mixed support of intron positions by RNA-seq alignment evidence used in manual assertion" EXACT [] +is_a: ECO:0000346 {is_inferred="true"} ! support of intron positions by RNA-sequencing alignment evidence used in manual assertion +is_a: ECO:0006172 ! mixed support of intron positions by RNA-sequencing alignment evidence +intersection_of: ECO:0006172 ! mixed support of intron positions by RNA-sequencing alignment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +property_value: IAO:0000234 "NCBI-RefSeq" xsd:string +created_by: snadendla + +[Term] +id: ECO:0006175 +name: nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence +def: "A type of nuclear magnetic resonance spectroscopy evidence resulting from measuring the change in a certain type of NMR spectrum as a result of the spontenaous exchange of hydrogen and deuterium nuclei between the sample and the solvent." [PMID:20960033, PMID:28538145, url:https\://en.wikipedia.org/wiki/Hydrogen%E2%80%93deuterium_exchange#NMR_spectroscopy] +synonym: "NMR HDX" EXACT [] +is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006176 +name: proton-based nuclear magnetic resonance evidence +def: "A type of nuclear magnetic resonance spectroscopy evidence in which the electromagnetic singal being measured comes from the perturbation of hydrogen nuclei resulting in a one dimensional spectrum." [url:https\://en.wikipedia.org/wiki/Proton_nuclear_magnetic_resonance] +synonym: "1D NMR" BROAD [] +synonym: "proton NMR" EXACT [] +is_a: ECO:0006163 ! nuclear magnetic resonance spectroscopy evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006177 +name: circular dichroism evidence +def: "A type of spectrometry evidence derived from irradiating a sample with light (either visible, infrared or ultraviolet wavelengths) and observing the difference of absorption of left-handed and right-handed light by the sample by comparing the extent of circular polarization in the incident and the emitted light." [PMID:17464384, url:https\://en.wikipedia.org/wiki/Circular_dichroism] +synonym: "CD" EXACT [] +is_a: ECO:0005031 ! structure determination evidence +is_a: ECO:0005504 ! spectrometry evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006178 +name: synchrotron radiation circular dichroism evidence +def: "A type of circular dichroism evidence where the source of radiation is radially accelerated particles, most often moving on a circular path in a synchrotron, typically providing lower wavelengths and therefore higher signal-to-noise ratio compared to regular circular dichroism measurements." [PMID:20658968] +synonym: "SRCD" EXACT [] +is_a: ECO:0006177 ! circular dichroism evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006179 +name: far-UV circular dichroism evidence +def: "A type of circular dichroism evidence whose spectrum is in the range of 190-230 nm, providing information mainly about amide bonds and through which the relative proportion of secondary structure elements of a protein can be estimated." [PMID:17464384] +synonym: "far-UV CD" EXACT [] +is_a: ECO:0006177 ! circular dichroism evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006180 +name: near-UV circular dichroism evidence +def: "A type of circular dichroism evidence whose spectrum is in the range of 250-350 nm , providing information mainly about aromatic residues, i.e. phenylalanine, tyrosine and tryptophan, through which it provides information on the tertiary structure of a protein." [PMID:17464384] +synonym: "near-UV CD" EXACT [] +is_a: ECO:0006177 ! circular dichroism evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006181 +name: cryogenic electron microscopy evidence +def: "A type of electron microscopy evidence where the sample being studied has been cooled to cryogenic temperatures (typically below -153 degrees Celsius) prior to the experiment." [PMID:31078399, url:https\://en.wikipedia.org/wiki/Cryogenic_electron_microscopy] +synonym: "cryo-EM" EXACT [] +is_a: ECO:0005033 ! electron microscopy evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006182 +name: small-angle X-ray scattering evidence +def: "A type of light scattering evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight and structural transitions by irradiating the sample with an X-ray beam and the scattered intensity is probed at small angles (typically in the range of 0.1-10 degrees) by a detector." [PMID:26320411, url:https\://en.wikipedia.org/wiki/Small-angle_X-ray_scattering] +synonym: "SAXS" EXACT [] +is_a: ECO:0007061 ! light scattering evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006183 +name: particle scattering evidence +def: "A type of structure determination evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight, diffusion and interaction strength by irradiating the sample with a particle beam and the scattered intensity is probed at a certain angle by a detector." [DisProt:BalintMeszaros] +is_a: ECO:0005031 ! structure determination evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006184 +name: small-angle neutron scattering evidence +def: "A type of particle scattering evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight and structural transitions by irradiating the sample with a beam of thermal neutrons and the scattered intensity is probed at small angles (typically in the range of 0.1-10 degrees) by a detector." [PMID:17714935, url:https\://en.wikipedia.org/wiki/Small-angle_neutron_scattering] +synonym: "SANS" EXACT [] +is_a: ECO:0006183 ! particle scattering evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006185 +name: author inference +def: "A type of inferential evidence where an assertion is derived by the authors of a scientific publication from another assertion and/or primary data via logical inference or other rational means." [DisProt:BalintMeszaros] +is_a: ECO:0000204 ! author statement +is_a: ECO:0000361 ! inferential evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006186 +name: combinatorial experimental and author inference evidence contained in single publication +def: "A type of combinatorial experimental and author inference evidence where the experimental results and author inference are contained in a single publication. " [ECO:MG] +is_a: ECO:0007011 ! combinatorial experimental and author inference evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006187 +name: X-ray crystallography-based structural model with missing residue coordinates +def: "A type of X-ray cystallography evidence in which a structural model is built lacking coordinates for some residues indicating high flexibility or structural disorder in that area of the molecule." [DisProt:BalintMeszaros] +is_a: ECO:0001823 ! x-ray crystallography evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006188 +name: X-ray crystallography-based structural model with high relative B-factor values +def: "A type of X-ray cystallography evidence in which a structural model is built that contains high relative B-factor values indicating flexibility in that area of the molecule." [DisProt:BalintMeszaros] +is_a: ECO:0001823 ! x-ray crystallography evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006189 +name: cryogenic electron microscopy-based structural model with missing residue coordinates +def: "A type of cryogenic electron microscopy evidence in which a structural model is built lacking coordinates for some residues indicating high flexibility or structural disorder in that area of the molecule." [DisProt:BalintMeszaros] +is_a: ECO:0006181 ! cryogenic electron microscopy evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006190 +name: cryogenic electron microscopy-based structural model with high relative B-factor values +def: "A type of cryogenic electron microscopy evidence in which a structural model is built that contains high relative B-factor values indicating flexibility in that area of the molecule." [DisProt:BalintMeszaros] +is_a: ECO:0006181 ! cryogenic electron microscopy evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006191 +name: Fourier-transform infrared spectroscopy evidence +def: "A type of spectrophotometry evidence resulting from the evaluation of a molecule in a fluid or solid state by its ability to alter the transmission of infrared light, where the raw data collected is transformed into the specturm by Fourier transform." [url:https\://en.wikipedia.org/wiki/Fourier-transform_infrared_spectroscopy] +synonym: "FT-IR" EXACT [] +synonym: "FTIR" EXACT [] +is_a: ECO:0001126 ! spectrophotometry evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006192 +name: heat capacity-based evidence +def: "A type of direct assay evidence derived from measuring the amount of heat absorbed or released by a sample to monitor chemical reactions, phase transitions or other processes affecting the physical state or chemical composition." [url:https\://en.wikipedia.org/wiki/Calorimetry] +synonym: "calorimetry evidence" BROAD [] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006193 +name: differential scanning calorimetry evidence +def: "A type of heat capacity-based evidence where the heat required to increase the temperature of the sample is continuously monitored and compared to the heat required to increase the temperature of a reference (having a largely identical composition compared to the sample, such as the empty buffer compared to a protein solution) over a range of temperatures." [url:https\://en.wikipedia.org/wiki/Differential_scanning_calorimetry] +synonym: "DSC" EXACT [] +is_a: ECO:0006192 ! heat capacity-based evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006194 +name: selective antibody-based structural conformation evidence +def: "A type of immunodetection assay evidence that involves the use of antibodies that are selective to conformations of the epitope to which they bind in order to provide information on conformations present in a target molecule." [DisProt:BalintMeszaros] +is_a: ECO:0005031 ! structure determination evidence +is_a: ECO:0005593 ! immunodetection assay evidence +property_value: IAO:0000112 "Here, we describe the generation, characterisation, and utility of a monoclonal antibody that selectively binds with high affinity to the asymmetric TNF trimer–small molecule complex. The antibody helps to define the molecular dynamics of the apo TNF trimer, reveals the mode of action and specificity of the small molecule inhibitors, acts as a chaperone in solving the human TNF–TNFR1 complex crystal structure, and facilitates the measurement of small molecule target occupancy in complex biological samples." xsd:string {xref="PMID:33495445"} +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006195 +name: protein hydrogen-deuterium exchange mass spectrometry evidence +def: "A type of mass spectrometry evidence derived from measuring changes in mass associated with the isotopic exchange between amide hydrogens of the protein backbone and its surrounding solvent, providing information on the structural state, the dynamics and the intrinsic chemical properties of the underlying amino acid sequence." [PMID:31249422, url:https\://en.wikipedia.org/wiki/Hydrogen%E2%80%93deuterium_exchange#Mass_spectrometry] +synonym: "HDX-MS" EXACT [] +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006196 +name: nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence used in manual assertion +def: "A type of nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence that is used in a manual assertion." [ECO:SN] +synonym: "NMR HDX" EXACT [] +is_a: ECO:0006165 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in manual assertion +is_a: ECO:0006175 ! nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence +intersection_of: ECO:0006175 ! nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006197 +name: nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence used in automatic assertion +def: "A type of nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence that is used in an automatic assertion." [ECO:SN] +synonym: "NMR HDX" EXACT [] +is_a: ECO:0006164 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in automatic assertion +is_a: ECO:0006175 ! nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence +intersection_of: ECO:0006175 ! nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006198 +name: proton-based nuclear magnetic resonance evidence used in manual assertion +def: "A type of proton-based nuclear magnetic resonance evidence that is used in a manual assertion." [ECO:SN] +synonym: "1D NMR" BROAD [] +synonym: "proton NMR" EXACT [] +is_a: ECO:0006165 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in manual assertion +is_a: ECO:0006176 ! proton-based nuclear magnetic resonance evidence +intersection_of: ECO:0006176 ! proton-based nuclear magnetic resonance evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006199 +name: proton-based nuclear magnetic resonance evidence used in automatic assertion +def: "A type of proton-based nuclear magnetic resonance evidence that is used in an automatic assertion." [ECO:SN] +synonym: "1D NMR" BROAD [] +synonym: "proton NMR" EXACT [] +is_a: ECO:0006164 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in automatic assertion +is_a: ECO:0006176 ! proton-based nuclear magnetic resonance evidence +intersection_of: ECO:0006176 ! proton-based nuclear magnetic resonance evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006200 +name: circular dichroism evidence used in manual assertion +def: "A type of circular dichroism evidence that is used in a manual assertion." [ECO:SN] +synonym: "CD" EXACT [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0006177 ! circular dichroism evidence +intersection_of: ECO:0006177 ! circular dichroism evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006201 +name: circular dichroism evidence used in automatic assertion +def: "A type of circular dichroism evidence that is used in an automatic assertion." [ECO:SN] +synonym: "CD" EXACT [] +is_a: ECO:0006177 ! circular dichroism evidence +is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion +is_a: ECO:0007821 {is_inferred="true"} ! spectrometry evidence used in automatic assertion +intersection_of: ECO:0006177 ! circular dichroism evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006202 +name: synchrotron radiation circular dichroism evidence used in manual assertion +def: "A type of synchrotron radiation circular dichroism evidence that is used in a manual assertion." [ECO:SN] +synonym: "SRCD" EXACT [] +is_a: ECO:0006178 ! synchrotron radiation circular dichroism evidence +is_a: ECO:0006200 {is_inferred="true"} ! circular dichroism evidence used in manual assertion +intersection_of: ECO:0006178 ! synchrotron radiation circular dichroism evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006203 +name: synchrotron radiation circular dichroism evidence used in automatic assertion +def: "A type of synchrotron radiation circular dichroism evidence that is used in an automatic assertion." [ECO:SN] +synonym: "SRCD" EXACT [] +is_a: ECO:0006178 ! synchrotron radiation circular dichroism evidence +is_a: ECO:0006201 {is_inferred="true"} ! circular dichroism evidence used in automatic assertion +intersection_of: ECO:0006178 ! synchrotron radiation circular dichroism evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006204 +name: far-UV circular dichroism evidence used in manual assertion +def: "A type of far-UV circular dichroism evidence that is used in a manual assertion." [ECO:SN] +synonym: "far-UV CD" EXACT [] +is_a: ECO:0006179 ! far-UV circular dichroism evidence +is_a: ECO:0006200 {is_inferred="true"} ! circular dichroism evidence used in manual assertion +intersection_of: ECO:0006179 ! far-UV circular dichroism evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006205 +name: far-UV circular dichroism evidence used in automatic assertion +def: "A type of far-UV circular dichroism evidence that is used in an automatic assertion." [ECO:SN] +synonym: "far-UV CD" EXACT [] +is_a: ECO:0006179 ! far-UV circular dichroism evidence +is_a: ECO:0006201 {is_inferred="true"} ! circular dichroism evidence used in automatic assertion +intersection_of: ECO:0006179 ! far-UV circular dichroism evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006206 +name: near-UV circular dichroism evidence used in manual assertion +def: "A type of near-UV circular dichroism evidence that is used in a manual assertion." [ECO:SN] +synonym: "near-UV CD" EXACT [] +is_a: ECO:0006180 ! near-UV circular dichroism evidence +is_a: ECO:0006200 {is_inferred="true"} ! circular dichroism evidence used in manual assertion +intersection_of: ECO:0006180 ! near-UV circular dichroism evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006207 +name: near-UV circular dichroism evidence used in automatic assertion +def: "A type of near-UV circular dichroism evidence that is used in an automatic assertion." [ECO:SN] +synonym: "near-UV CD" EXACT [] +is_a: ECO:0006180 ! near-UV circular dichroism evidence +is_a: ECO:0006201 {is_inferred="true"} ! circular dichroism evidence used in automatic assertion +intersection_of: ECO:0006180 ! near-UV circular dichroism evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006208 +name: cryogenic electron microscopy evidence used in manual assertion +def: "A type of cryogenic electron microscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "cryo-EM" EXACT [] +is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion +is_a: ECO:0006181 ! cryogenic electron microscopy evidence +intersection_of: ECO:0006181 ! cryogenic electron microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006209 +name: cryogenic electron microscopy evidence used in automatic assertion +def: "A type of cryogenic electron microscopy evidence that is used in an automatic assertion." [ECO:SN] +synonym: "cryo-EM" EXACT [] +is_a: ECO:0006181 ! cryogenic electron microscopy evidence +is_a: ECO:0007493 {is_inferred="true"} ! electron microscopy evidence used in automatic assertion +intersection_of: ECO:0006181 ! cryogenic electron microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006210 +name: small-angle X-ray scattering evidence used in manual assertion +def: "A type of small-angle X-ray scattering evidence that is used in a manual assertion." [ECO:SN] +synonym: "SAXS" EXACT [] +is_a: ECO:0006182 ! small-angle X-ray scattering evidence +is_a: ECO:0007062 {is_inferred="true"} ! light scattering evidence used in manual assertion +intersection_of: ECO:0006182 ! small-angle X-ray scattering evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006211 +name: small-angle X-ray scattering evidence used in automatic assertion +def: "A type of small-angle X-ray scattering evidence that is used in an automatic assertion." [ECO:SN] +synonym: "SAXS" EXACT [] +is_a: ECO:0006182 ! small-angle X-ray scattering evidence +is_a: ECO:0007617 {is_inferred="true"} ! light scattering evidence used in automatic assertion +intersection_of: ECO:0006182 ! small-angle X-ray scattering evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006212 +name: particle scattering evidence used in manual assertion +def: "A type of particle scattering evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0006183 ! particle scattering evidence +intersection_of: ECO:0006183 ! particle scattering evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006213 +name: particle scattering evidence used in automatic assertion +def: "A type of particle scattering evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006183 ! particle scattering evidence +is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion +intersection_of: ECO:0006183 ! particle scattering evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006214 +name: small-angle neutron scattering evidence used in manual assertion +def: "A type of small-angle neutron scattering evidence that is used in a manual assertion." [ECO:SN] +synonym: "SANS" EXACT [] +is_a: ECO:0006184 ! small-angle neutron scattering evidence +is_a: ECO:0006212 {is_inferred="true"} ! particle scattering evidence used in manual assertion +intersection_of: ECO:0006184 ! small-angle neutron scattering evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006215 +name: small-angle neutron scattering evidence used in automatic assertion +def: "A type of small-angle neutron scattering evidence that is used in an automatic assertion." [ECO:SN] +synonym: "SANS" EXACT [] +is_a: ECO:0006184 ! small-angle neutron scattering evidence +is_a: ECO:0006213 {is_inferred="true"} ! particle scattering evidence used in automatic assertion +intersection_of: ECO:0006184 ! small-angle neutron scattering evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006216 +name: author inference used in manual assertion +def: "A type of author inference that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000302 {is_inferred="true"} ! author statement used in manual assertion +is_a: ECO:0006185 ! author inference +is_a: ECO:0007751 {is_inferred="true"} ! inferential evidence used in manual assertion +intersection_of: ECO:0006185 ! author inference +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006217 +name: author inference used in automatic assertion +def: "A type of author inference that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006185 ! author inference +is_a: ECO:0007319 {is_inferred="true"} ! author statement used in automatic assertion +is_a: ECO:0007832 {is_inferred="true"} ! inferential evidence used in automatic assertion +intersection_of: ECO:0006185 ! author inference +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006218 +name: combinatorial experimental and author inference evidence contained in single publication used in manual assertion +def: "A type of combinatorial experimental and author inference evidence contained in single publication that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006186 ! combinatorial experimental and author inference evidence contained in single publication +is_a: ECO:0007013 {is_inferred="true"} ! combinatorial experimental and author inference evidence used in manual assertion +intersection_of: ECO:0006186 ! combinatorial experimental and author inference evidence contained in single publication +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006219 +name: combinatorial experimental and author inference evidence contained in single publication used in automatic assertion +def: "A type of combinatorial experimental and author inference evidence contained in single publication that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006186 ! combinatorial experimental and author inference evidence contained in single publication +is_a: ECO:0007592 {is_inferred="true"} ! combinatorial experimental and author inference evidence used in automatic assertion +intersection_of: ECO:0006186 ! combinatorial experimental and author inference evidence contained in single publication +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006220 +name: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion +def: "A type of X-ray crystallography-based structural model with missing residue coordinates that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005670 {is_inferred="true"} ! x-ray crystallography evidence used in manual assertion +is_a: ECO:0006187 ! X-ray crystallography-based structural model with missing residue coordinates +intersection_of: ECO:0006187 ! X-ray crystallography-based structural model with missing residue coordinates +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006221 +name: X-ray crystallography-based structural model with missing residue coordinates used in automatic assertion +def: "A type of X-ray crystallography-based structural model with missing residue coordinates that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0005671 {is_inferred="true"} ! x-ray crystallography evidence used in automatic assertion +is_a: ECO:0006187 ! X-ray crystallography-based structural model with missing residue coordinates +intersection_of: ECO:0006187 ! X-ray crystallography-based structural model with missing residue coordinates +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006222 +name: X-ray crystallography-based structural model with high relative B-factor values used in manual assertion +def: "A type of X-ray crystallography-based structural model with high relative B-factor values that is used in a manual assertion." [ECO:SN] +is_a: ECO:0005670 {is_inferred="true"} ! x-ray crystallography evidence used in manual assertion +is_a: ECO:0006188 ! X-ray crystallography-based structural model with high relative B-factor values +intersection_of: ECO:0006188 ! X-ray crystallography-based structural model with high relative B-factor values +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006223 +name: X-ray crystallography-based structural model with high relative B-factor values used in automatic assertion +def: "A type of X-ray crystallography-based structural model with high relative B-factor values that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0005671 {is_inferred="true"} ! x-ray crystallography evidence used in automatic assertion +is_a: ECO:0006188 ! X-ray crystallography-based structural model with high relative B-factor values +intersection_of: ECO:0006188 ! X-ray crystallography-based structural model with high relative B-factor values +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006224 +name: cryogenic electron microscopy-based structural model with missing residue coordinates used in manual assertion +def: "A type of cryogenic electron microscopy-based structural model with missing residue coordinates that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006189 ! cryogenic electron microscopy-based structural model with missing residue coordinates +is_a: ECO:0006208 {is_inferred="true"} ! cryogenic electron microscopy evidence used in manual assertion +intersection_of: ECO:0006189 ! cryogenic electron microscopy-based structural model with missing residue coordinates +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006225 +name: cryogenic electron microscopy-based structural model with missing residue coordinates used in automatic assertion +def: "A type of cryogenic electron microscopy-based structural model with missing residue coordinates that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006189 ! cryogenic electron microscopy-based structural model with missing residue coordinates +is_a: ECO:0006209 {is_inferred="true"} ! cryogenic electron microscopy evidence used in automatic assertion +intersection_of: ECO:0006189 ! cryogenic electron microscopy-based structural model with missing residue coordinates +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006226 +name: cryogenic electron microscopy-based structural model with high relative B-factor values used in manual assertion +def: "A type of cryogenic electron microscopy-based structural model with high relative B-factor values that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006190 ! cryogenic electron microscopy-based structural model with high relative B-factor values +is_a: ECO:0006208 {is_inferred="true"} ! cryogenic electron microscopy evidence used in manual assertion +intersection_of: ECO:0006190 ! cryogenic electron microscopy-based structural model with high relative B-factor values +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006227 +name: cryogenic electron microscopy-based structural model with high relative B-factor values used in automatic assertion +def: "A type of cryogenic electron microscopy-based structural model with high relative B-factor values that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006190 ! cryogenic electron microscopy-based structural model with high relative B-factor values +is_a: ECO:0006209 {is_inferred="true"} ! cryogenic electron microscopy evidence used in automatic assertion +intersection_of: ECO:0006190 ! cryogenic electron microscopy-based structural model with high relative B-factor values +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006228 +name: Fourier-transform infrared spectroscopy evidence used in manual assertion +def: "A type of Fourier-transform infrared spectroscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "FT-IR" EXACT [] +synonym: "FTIR" EXACT [] +is_a: ECO:0001258 {is_inferred="true"} ! spectrophotometry evidence used in manual assertion +is_a: ECO:0006191 ! Fourier-transform infrared spectroscopy evidence +intersection_of: ECO:0006191 ! Fourier-transform infrared spectroscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006229 +name: Fourier-transform infrared spectroscopy evidence used in automatic assertion +def: "A type of Fourier-transform infrared spectroscopy evidence that is used in an automatic assertion." [ECO:SN] +synonym: "FT-IR" EXACT [] +synonym: "FTIR" EXACT [] +is_a: ECO:0006191 ! Fourier-transform infrared spectroscopy evidence +is_a: ECO:0007458 {is_inferred="true"} ! spectrophotometry evidence used in automatic assertion +intersection_of: ECO:0006191 ! Fourier-transform infrared spectroscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006230 +name: heat capacity-based evidence used in manual assertion +def: "A type of heat capacity-based evidence that is used in a manual assertion." [ECO:SN] +synonym: "calorimetry evidence" BROAD [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0006192 ! heat capacity-based evidence +intersection_of: ECO:0006192 ! heat capacity-based evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006231 +name: heat capacity-based evidence used in automatic assertion +def: "A type of heat capacity-based evidence that is used in an automatic assertion." [ECO:SN] +synonym: "calorimetry evidence" BROAD [] +is_a: ECO:0006192 ! heat capacity-based evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0006192 ! heat capacity-based evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006232 +name: differential scanning calorimetry evidence used in manual assertion +def: "A type of differential scanning calorimetry evidence that is used in a manual assertion." [ECO:SN] +synonym: "DSC" EXACT [] +is_a: ECO:0006193 ! differential scanning calorimetry evidence +is_a: ECO:0006230 {is_inferred="true"} ! heat capacity-based evidence used in manual assertion +intersection_of: ECO:0006193 ! differential scanning calorimetry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006233 +name: differential scanning calorimetry evidence used in automatic assertion +def: "A type of differential scanning calorimetry evidence that is used in an automatic assertion." [ECO:SN] +synonym: "DSC" EXACT [] +is_a: ECO:0006193 ! differential scanning calorimetry evidence +is_a: ECO:0006231 {is_inferred="true"} ! heat capacity-based evidence used in automatic assertion +intersection_of: ECO:0006193 ! differential scanning calorimetry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006234 +name: selective antibody-based structural conformation evidence used in manual assertion +def: "A type of selective antibody-based structural conformation evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0006194 ! selective antibody-based structural conformation evidence +intersection_of: ECO:0006194 ! selective antibody-based structural conformation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006235 +name: selective antibody-based structural conformation evidence used in automatic assertion +def: "A type of selective antibody-based structural conformation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006194 ! selective antibody-based structural conformation evidence +is_a: ECO:0007796 {is_inferred="true"} ! immunodetection assay evidence used in automatic assertion +is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion +intersection_of: ECO:0006194 ! selective antibody-based structural conformation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006236 +name: protein hydrogen-deuterium exchange mass spectrometry evidence used in manual assertion +def: "A type of protein hydrogen-deuterium exchange mass spectrometry evidence that is used in a manual assertion." [ECO:SN] +synonym: "HDX-MS" EXACT [] +is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion +is_a: ECO:0006195 ! protein hydrogen-deuterium exchange mass spectrometry evidence +intersection_of: ECO:0006195 ! protein hydrogen-deuterium exchange mass spectrometry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006237 +name: protein hydrogen-deuterium exchange mass spectrometry evidence used in automatic assertion +def: "A type of protein hydrogen-deuterium exchange mass spectrometry evidence that is used in an automatic assertion." [ECO:SN] +synonym: "HDX-MS" EXACT [] +is_a: ECO:0006195 ! protein hydrogen-deuterium exchange mass spectrometry evidence +is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion +intersection_of: ECO:0006195 ! protein hydrogen-deuterium exchange mass spectrometry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006238 +name: galactokinase reporter gene assay evidence +def: "A type of reporter gene assay evidence based on the fusion of the galK gene to a specific promoter for the expression of the enzyme galactokinase." [url:https\://www.sciencedirect.com/science/article/pii/007668796608039X] +is_a: ECO:0000049 ! reporter gene assay evidence +property_value: IAO:0000234 OMP:DebbySiegele xsd:string +created_by: snadendla + +[Term] +id: ECO:0006239 +name: polyadenylated transcript 3'-end-sequencing evidence +def: "A type of RNA-sequencing evidence based on sequencing of the 3' ends of cDNA molecules that are selected by presence of a polyadenylated tail." [PMID:31617559] +is_a: ECO:0000295 ! RNA-sequencing evidence +property_value: IAO:0000234 NCBI-RefSeq:MurphyTerence xsd:string +created_by: snadendla + +[Term] +id: ECO:0006240 +name: colony boundary assay evidence +def: "A type of colony morphology phenotypic evidence based on the presence or absence of a visually apparent boundary formed between colonies of multiple isolates or strains of the same microbial species growing on a solid surface such as that of an agar culture medium." [PMID:18621670] +is_a: ECO:0007077 ! colony morphology phenotypic evidence +property_value: IAO:0000234 OMP:DebbySiegele xsd:string +created_by: snadendla + +[Term] +id: ECO:0006241 +name: galactokinase reporter gene assay evidence used in manual assertion +def: "A type of galactokinase reporter gene assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0006238 ! galactokinase reporter gene assay evidence +intersection_of: ECO:0006238 ! galactokinase reporter gene assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +property_value: IAO:0000234 OMP:DebbySiegele xsd:string +created_by: snadendla + +[Term] +id: ECO:0006242 +name: galactokinase reporter gene assay evidence used in automatic assertion +def: "A type of galactokinase reporter gene assay evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006238 ! galactokinase reporter gene assay evidence +is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion +intersection_of: ECO:0006238 ! galactokinase reporter gene assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 OMP:DebbySiegele xsd:string +created_by: snadendla + +[Term] +id: ECO:0006243 +name: polyadenylated transcript 3'-end-sequencing evidence used in manual assertion +def: "A type of polyadenylated transcript 3'-end-sequencing evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006068 {is_inferred="true"} ! RNA-sequencing evidence used in manual assertion +is_a: ECO:0006239 ! polyadenylated transcript 3'-end-sequencing evidence +intersection_of: ECO:0006239 ! polyadenylated transcript 3'-end-sequencing evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +property_value: IAO:0000234 NCBI-RefSeq:MurphyTerence xsd:string +created_by: snadendla + +[Term] +id: ECO:0006244 +name: polyadenylated transcript 3'-end-sequencing evidence used in automatic assertion +def: "A type of polyadenylated transcript 3'-end-sequencing evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006069 {is_inferred="true"} ! RNA-sequencing evidence used in automatic assertion +is_a: ECO:0006239 ! polyadenylated transcript 3'-end-sequencing evidence +intersection_of: ECO:0006239 ! polyadenylated transcript 3'-end-sequencing evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 NCBI-RefSeq:MurphyTerence xsd:string +created_by: snadendla + +[Term] +id: ECO:0006245 +name: colony boundary assay evidence used in manual assertion +def: "A type of colony boundary assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006240 ! colony boundary assay evidence +is_a: ECO:0007078 {is_inferred="true"} ! colony morphology phenotypic evidence used in manual assertion +intersection_of: ECO:0006240 ! colony boundary assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 OMP:DebbySiegele xsd:string +created_by: snadendla + +[Term] +id: ECO:0006246 +name: colony boundary assay evidence used in automatic assertion +def: "A type of colony boundary assay evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006240 ! colony boundary assay evidence +is_a: ECO:0007625 {is_inferred="true"} ! colony morphology phenotypic evidence used in automatic assertion +intersection_of: ECO:0006240 ! colony boundary assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 OMP:DebbySiegele xsd:string +created_by: snadendla + +[Term] +id: ECO:0006247 +name: analytical ultracentrifugation evidence +def: "A type of molecule detection assay evidence where the hydrodynamic behaviour of macromolecules is used to study their size, shape and interactions, by spinning the sample solution at high centrifugal field (up to above 100000 rpm and 1000000 g), and monitoring the evolution of sample concentration profile versus the axis of rotation." [PMID:12192063] +synonym: "AUC" EXACT [] +is_a: ECO:0005516 ! molecule detection assay evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006248 +name: fluorescence polarization evidence +def: "A type of physical interaction evidence based on the measurement of the change in the degree of polarization of a fluorophore upon binding to a molecular partner." [PMID:21372817] +synonym: "FP" EXACT [] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006249 +name: obsolete bait-prey protein pull-down evidence +def: "OBSOLETE A type of protein binding evidence resulting from an affinity purification in-vitro technique used to detect physical interactions between two or more proteins, where a molecule of interest - a \"bait\" - is immobilized and incubated with a protein source containing putative \"prey\" proteins." [PMID:28667618] +comment: This term has been obsoleted as this is same as the term bait-prey hybrid interaction evidence. +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +is_obsolete: true +created_by: snadendla + +[Term] +id: ECO:0006250 +name: rotary shadowing electron microscopy evidence +def: "A type of electron microscopy evidence where a heavy metal is evaporated onto surface adsorbed molecules and macromolecular assemblies allowing high-contrast visualization of both individual macromolecules and the surface structure of macromolecular assemblies." [PMID:19247619] +synonym: "RS-TEM" EXACT [] +is_a: ECO:0005033 ! electron microscopy evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006251 +name: electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence +def: "A type of mass spectrometry evidence resulting from the ionization of polar functional groups producing multiple charged ions followed by the detection of ion cyclotron frequencies within a magnetic field, making it suitable for the study of large molecules (>2 kDa)." [PMID:10575730] +synonym: "ESI FT-ICR MS" EXACT [] +is_a: ECO:0001096 ! mass spectrometry evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006252 +name: magnetic resonance evidence +def: "A type of experimental evidence in which particles in strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting eletromagnetic signal is captured." [ECO:MG] +is_a: ECO:0000006 ! experimental evidence +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006253 +name: electron paramagnetic resonance evidence +def: "A type of magnetic resonance evidence in which unpaired electrons in a strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting electromagnetic signal is captured." [PMID:21826602, url:https\://en.wikipedia.org/wiki/Electron_paramagnetic_resonance] +synonym: "EPR" EXACT [] +synonym: "ESR" EXACT [] +is_a: ECO:0006252 ! magnetic resonance evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006254 +name: site-directed spin-labelling electron paramagnetic resonance evidence +def: "A type of electron paramagnetic resonance evidence where the signal is obtained by labeling the protein in specific sites with a functional group (most often a nitroxide) containing an unpaired electron using site-directed mutagenesis." [PMID:21826602, url:https\://en.wikipedia.org/wiki/Site-directed_spin_labeling] +synonym: "SDSL" EXACT [] +is_a: ECO:0006253 ! electron paramagnetic resonance evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006255 +name: deglycosylation assay evidence +def: "A type of direct assay evidence where the removal of glycan groups from a substrate (RNA, DNA, or protein) is detected." [url:https\://en.wikipedia.org/wiki/Glycosylation] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006256 +name: protein fragment functional complementation evidence +def: "A type of physical interaction evidence that is based on the detection of a protein-protein interaction between a bait and prey protein by reconstitution of a reporter protein serving as a detectable entity (usually an enzyme or a fluorescent protein), the two halves of which are each attached separately to the bait and prey." [PMID:31274323] +is_a: ECO:0000021 ! physical interaction evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006257 +name: beta galactosidase functional complementation evidence +def: "A type of protein fragment functional complementaion evidence where the interaction between bait and prey assemble a functional beta galactosidase enzyme." [PMID:9237989] +is_a: ECO:0006256 ! protein fragment functional complementation evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006258 +name: GAL4-VP16 functional complementation evidence +def: "A type of protein fragment functional complementaion evidence where the interaction between bait and prey assemble a transcriptional activation complex consisting of the GAL4 DNA-binding domain and the transactivation domain from the herpes simplex virus protein VP16." [PSI-MI:0728] +is_a: ECO:0006256 ! protein fragment functional complementation evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006259 +name: Raman spectroscopy evidence +def: "A type of spectrophotometry evidence used to determine a structural fingerprint of the molecule in solution by probing its vibrational modes via the inelastic scattering of photons (called Raman scattering)." [url:https\://en.wikipedia.org/wiki/Raman_spectroscopy] +synonym: "Raman optical activity" EXACT [] +synonym: "ROA" EXACT [] +is_a: ECO:0001126 ! spectrophotometry evidence +is_a: ECO:0005031 ! structure determination evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006260 +name: protein thermal shift assay evidence +def: "A type of heat capacity-based evidence in which the denaturation temperature of a protein sample is measured under varying conditions, such as pH, redox potential, concentration of other molecules or changes in the proteoform, to determine the effect of these factors on protein thermal stability." [url:https\://en.wikipedia.org/wiki/Thermal_shift_assay] +is_a: ECO:0006192 ! heat capacity-based evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006261 +name: microscale thermophoresis evidence +def: "A type of physical interaction evidence resulting from measuring the change of the fluorescent activity of a molecule as a function of binding to a non-fluorescent partner and its movement in a microspcopic thermal gradient affecting the intensity of fluorescence." [PMID:23270813, url:https\://en.wikipedia.org/wiki/Microscale_thermophoresis] +synonym: "MST" EXACT [] +is_a: ECO:0000021 ! physical interaction evidence +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006262 +name: native protein gel electrophoresis evidence +def: "A type of gel electrophoresis evidence resulting from the use of non-denaturing conditions so that the proteins being measured are in their native structural states." [url:https\://en.wikipedia.org/wiki/Gel_electrophoresis_of_proteins] +synonym: "BN-PAGE" NARROW [] +synonym: "CN-PAGE" NARROW [] +synonym: "native gel" EXACT [] +synonym: "native PAGE" NARROW [] +synonym: "QPNC-PAGE" NARROW [] +is_a: ECO:0000337 ! gel electrophoresis evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006263 +name: turbidity measurement evidence +def: "A type of direct assay evidence where the optical properties of a solution is measured in the visible light spectrum, assessing the haziness or cloudiness as a result of the properties of the solutes or the changes in their material states." [url:https\://en.wikipedia.org/wiki/Turbidity] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006264 +name: competitive binding evidence +def: "A type of affinity evidence derived from an experimental setup where the binding properties of a ligand are assessed by measuring the competition with another ligand with already known binding properties." [PMID:21115850] +synonym: "competition assay" EXACT [] +is_a: ECO:0000023 ! affinity evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006265 +name: protein unfolding evidence +def: "A type of structure determination evidence obtained by monitoring structural changes of a protein as a response to changes of an environmental parameter, such as pH or temperature, or the concentration of a denaturant, such as urea." [url:https\://en.wikipedia.org/wiki/Equilibrium_unfolding] +synonym: "protein denaturation assay" EXACT [] +is_a: ECO:0005031 ! structure determination evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006266 +name: urea-induced protein unfolding evidence +def: "A type of protein unfolding evidence where the unfolding of the protein is triggered by increasing concentrations of urea." [PMID:19708649] +synonym: "chemical denaturation" BROAD [] +synonym: "chemical unfolding" BROAD [] +is_a: ECO:0006265 ! protein unfolding evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006267 +name: pH-induced protein unfolding evidence +def: "A type of protein unfolding evidence where the unfolding of the protein is triggered by changes in pH." [PMID:23185611] +is_a: ECO:0006265 ! protein unfolding evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006268 +name: temperature-induced protein unfolding evidence +def: "A type of protein unfolding evidence where the unfolding of the protein is triggered by changes in temperature." [PMID:23185611] +synonym: "thermal dentauration" EXACT [] +synonym: "thermal unfolding" EXACT [] +is_a: ECO:0006265 ! protein unfolding evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006269 +name: cell aggregation evidence +def: "A type of cell-based assay evidence resulting from the analysis of cells irreversibly binding to each other." [PMID:24092433] +is_a: ECO:0001565 ! cell-based assay evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006270 +name: RNA-protein binding evidence +def: "A type of affinity evidence resulting from the qualitative and quantitative analysis of the affinity with which a protein binds to RNA." [PMID:30804549] +is_a: ECO:0000024 ! protein binding evidence +is_a: ECO:0000136 ! nucleic acid binding evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006271 +name: fluorescence microscopy evidence +def: "A type of microscopy evidence that is obtained by using the fluorescence of the studied sample instead of or in addition to the scattering, reflection, attenuation or absorption of light." [url:https\://en.wikipedia.org/wiki/Fluorescence_microscope] +is_a: ECO:0001098 ! microscopy evidence +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006272 +name: viscosity measurement evidence +def: "A type of direct assay evidence where the viscosity of a solution is measured to quantify the material properties of the solution, or the changes of these properties in response to changing composition or environmental factors." [url:https\://en.wikipedia.org/wiki/Viscometer] +synonym: "viscometry" EXACT [] +is_a: ECO:0000002 ! direct assay evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006273 +name: dynamic fluorescence quenching evidence +def: "A type of physical interaction evidence derived from the decrease (quenching) of fluorescence as a result of the interaction between a fluorescence donor and acceptor in their excited state." [url:https\://en.wikipedia.org/wiki/Quenching_(fluorescence)] +synonym: "Dexter" NARROW [] +synonym: "exciplex" NARROW [] +synonym: "FRET" NARROW [] +synonym: "quenching" BROAD [] +is_a: ECO:0000021 ! physical interaction evidence +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006274 +name: static fluorescence quenching evidence +def: "A type of physical interaction evidence derived from the decrease (quenching) of fluorescence as a result of the intramolecular interaction between a fluorescence donor and acceptor creating a non-fluorescent ground state." [url:https\://en.wikipedia.org/wiki/Quenching_(fluorescence)] +synonym: "quenching" BROAD [] +is_a: ECO:0000021 ! physical interaction evidence +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006275 +name: analytical ultracentrifugation evidence used in manual assertion +def: "A type of analytical ultracentrifugation evidence that is used in a manual assertion." [ECO:SN] +synonym: "AUC" EXACT [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0006247 ! analytical ultracentrifugation evidence +intersection_of: ECO:0006247 ! analytical ultracentrifugation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006276 +name: analytical ultracentrifugation evidence used in automatic assertion +def: "A type of analytical ultracentrifugation evidence that is used in an automatic assertion." [ECO:SN] +synonym: "AUC" EXACT [] +is_a: ECO:0006247 ! analytical ultracentrifugation evidence +is_a: ECO:0007816 {is_inferred="true"} ! molecule detection assay evidence used in automatic assertion +intersection_of: ECO:0006247 ! analytical ultracentrifugation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006277 +name: fluorescence polarization evidence used in manual assertion +def: "A type of fluorescence polarization evidence that is used in a manual assertion." [ECO:SN] +synonym: "FP" EXACT [] +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +is_a: ECO:0006248 ! fluorescence polarization evidence +intersection_of: ECO:0006248 ! fluorescence polarization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006278 +name: fluorescence polarization evidence used in automatic assertion +def: "A type of fluorescence polarization evidence that is used in an automatic assertion." [ECO:SN] +synonym: "FP" EXACT [] +is_a: ECO:0006248 ! fluorescence polarization evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +intersection_of: ECO:0006248 ! fluorescence polarization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006279 +name: obsolete bait-prey protein pull-down evidence used in manual assertion +def: "OBSOLETE A type of bait-prey protein pull-down evidence that is used in a manual assertion." [ECO:SN] +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +is_obsolete: true +created_by: snadendla + +[Term] +id: ECO:0006280 +name: obsolete bait-prey protein pull-down evidence used in automatic assertion +def: "OBSOLETE A type of bait-prey protein pull-down evidence that is used in an automatic assertion." [ECO:SN] +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +is_obsolete: true +created_by: snadendla + +[Term] +id: ECO:0006281 +name: rotary shadowing electron microscopy evidence used in manual assertion +def: "A type of rotary shadowing electron microscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "RS-TEM" EXACT [] +is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion +is_a: ECO:0006250 ! rotary shadowing electron microscopy evidence +intersection_of: ECO:0006250 ! rotary shadowing electron microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006282 +name: rotary shadowing electron microscopy evidence used in automatic assertion +def: "A type of rotary shadowing electron microscopy evidence that is used in an automatic assertion." [ECO:SN] +synonym: "RS-TEM" EXACT [] +is_a: ECO:0006250 ! rotary shadowing electron microscopy evidence +is_a: ECO:0007493 {is_inferred="true"} ! electron microscopy evidence used in automatic assertion +intersection_of: ECO:0006250 ! rotary shadowing electron microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006283 +name: electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence used in manual assertion +def: "A type of electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence that is used in a manual assertion." [ECO:SN] +synonym: "ESI FT-ICR MS" EXACT [] +is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion +is_a: ECO:0006251 ! electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence +intersection_of: ECO:0006251 ! electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006284 +name: electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence used in automatic assertion +def: "A type of electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence that is used in an automatic assertion." [ECO:SN] +synonym: "ESI FT-ICR MS" EXACT [] +is_a: ECO:0006251 ! electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence +is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion +intersection_of: ECO:0006251 ! electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006285 +name: magnetic resonance evidence used in manual assertion +def: "A type of magnetic resonance evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0006252 ! magnetic resonance evidence +intersection_of: ECO:0006252 ! magnetic resonance evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006286 +name: magnetic resonance evidence used in automatic assertion +def: "A type of magnetic resonance evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006252 ! magnetic resonance evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0006252 ! magnetic resonance evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:FedericaQuaglia xsd:string +created_by: snadendla + +[Term] +id: ECO:0006287 +name: electron paramagnetic resonance evidence used in manual assertion +def: "A type of electron paramagnetic resonance evidence that is used in a manual assertion." [ECO:SN] +synonym: "EPR" EXACT [] +synonym: "ESR" EXACT [] +is_a: ECO:0006253 ! electron paramagnetic resonance evidence +is_a: ECO:0006285 {is_inferred="true"} ! magnetic resonance evidence used in manual assertion +intersection_of: ECO:0006253 ! electron paramagnetic resonance evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006288 +name: electron paramagnetic resonance evidence used in automatic assertion +def: "A type of electron paramagnetic resonance evidence that is used in an automatic assertion." [ECO:SN] +synonym: "EPR" EXACT [] +synonym: "ESR" EXACT [] +is_a: ECO:0006253 ! electron paramagnetic resonance evidence +is_a: ECO:0006286 {is_inferred="true"} ! magnetic resonance evidence used in automatic assertion +intersection_of: ECO:0006253 ! electron paramagnetic resonance evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006289 +name: site-directed spin-labelling electron paramagnetic resonance evidence used in manual assertion +def: "A type of site-directed spin-labelling electron paramagnetic resonance evidence that is used in a manual assertion." [ECO:SN] +synonym: "SDSL" EXACT [] +is_a: ECO:0006254 ! site-directed spin-labelling electron paramagnetic resonance evidence +is_a: ECO:0006287 {is_inferred="true"} ! electron paramagnetic resonance evidence used in manual assertion +intersection_of: ECO:0006254 ! site-directed spin-labelling electron paramagnetic resonance evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006290 +name: site-directed spin-labelling electron paramagnetic resonance evidence used in automatic assertion +def: "A type of site-directed spin-labelling electron paramagnetic resonance evidence that is used in an automatic assertion." [ECO:SN] +synonym: "SDSL" EXACT [] +is_a: ECO:0006254 ! site-directed spin-labelling electron paramagnetic resonance evidence +is_a: ECO:0006288 {is_inferred="true"} ! electron paramagnetic resonance evidence used in automatic assertion +intersection_of: ECO:0006254 ! site-directed spin-labelling electron paramagnetic resonance evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006291 +name: deglycosylation assay evidence used in manual assertion +def: "A type of deglycosylation assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0006255 ! deglycosylation assay evidence +intersection_of: ECO:0006255 ! deglycosylation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006292 +name: deglycosylation assay evidence used in automatic assertion +def: "A type of deglycosylation assay evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006255 ! deglycosylation assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0006255 ! deglycosylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006293 +name: protein fragment functional complementation evidence used in manual assertion +def: "A type of protein fragment functional complementation evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +is_a: ECO:0006256 ! protein fragment functional complementation evidence +intersection_of: ECO:0006256 ! protein fragment functional complementation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006294 +name: protein fragment functional complementation evidence used in automatic assertion +def: "A type of protein fragment functional complementation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006256 ! protein fragment functional complementation evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +intersection_of: ECO:0006256 ! protein fragment functional complementation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006295 +name: beta galactosidase functional complementation evidence used in manual assertion +def: "A type of beta galactosidase functional complementation evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006257 ! beta galactosidase functional complementation evidence +is_a: ECO:0006293 {is_inferred="true"} ! protein fragment functional complementation evidence used in manual assertion +intersection_of: ECO:0006257 ! beta galactosidase functional complementation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006296 +name: beta galactosidase functional complementation evidence used in automatic assertion +def: "A type of beta galactosidase functional complementation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006257 ! beta galactosidase functional complementation evidence +is_a: ECO:0006294 {is_inferred="true"} ! protein fragment functional complementation evidence used in automatic assertion +intersection_of: ECO:0006257 ! beta galactosidase functional complementation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006297 +name: GAL4-VP16 functional complementation evidence used in manual assertion +def: "A type of GAL4-VP16 functional complementation evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006258 ! GAL4-VP16 functional complementation evidence +is_a: ECO:0006293 {is_inferred="true"} ! protein fragment functional complementation evidence used in manual assertion +intersection_of: ECO:0006258 ! GAL4-VP16 functional complementation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006298 +name: GAL4-VP16 functional complementation evidence used in automatic assertion +def: "A type of GAL4-VP16 functional complementation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006258 ! GAL4-VP16 functional complementation evidence +is_a: ECO:0006294 {is_inferred="true"} ! protein fragment functional complementation evidence used in automatic assertion +intersection_of: ECO:0006258 ! GAL4-VP16 functional complementation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006299 +name: Raman spectroscopy evidence used in manual assertion +def: "A type of Raman spectroscopy evidence that is used in a manual assertion." [ECO:SN] +synonym: "Raman optical activity" EXACT [] +synonym: "ROA" EXACT [] +is_a: ECO:0001258 {is_inferred="true"} ! spectrophotometry evidence used in manual assertion +is_a: ECO:0006259 ! Raman spectroscopy evidence +intersection_of: ECO:0006259 ! Raman spectroscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006300 +name: Raman spectroscopy evidence used in automatic assertion +def: "A type of Raman spectroscopy evidence that is used in an automatic assertion." [ECO:SN] +synonym: "Raman optical activity" EXACT [] +synonym: "ROA" EXACT [] +is_a: ECO:0006259 ! Raman spectroscopy evidence +is_a: ECO:0007458 {is_inferred="true"} ! spectrophotometry evidence used in automatic assertion +is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion +intersection_of: ECO:0006259 ! Raman spectroscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006301 +name: protein thermal shift assay evidence used in manual assertion +def: "A type of protein thermal shift assay evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006230 {is_inferred="true"} ! heat capacity-based evidence used in manual assertion +is_a: ECO:0006260 ! protein thermal shift assay evidence +intersection_of: ECO:0006260 ! protein thermal shift assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006302 +name: protein thermal shift assay evidence used in automatic assertion +def: "A type of protein thermal shift assay evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006231 {is_inferred="true"} ! heat capacity-based evidence used in automatic assertion +is_a: ECO:0006260 ! protein thermal shift assay evidence +intersection_of: ECO:0006260 ! protein thermal shift assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006303 +name: microscale thermophoresis evidence used in manual assertion +def: "A type of microscale thermophoresis evidence that is used in a manual assertion." [ECO:SN] +synonym: "MST" EXACT [] +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion +is_a: ECO:0006261 ! microscale thermophoresis evidence +intersection_of: ECO:0006261 ! microscale thermophoresis evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006304 +name: microscale thermophoresis evidence used in automatic assertion +def: "A type of microscale thermophoresis evidence that is used in an automatic assertion." [ECO:SN] +synonym: "MST" EXACT [] +is_a: ECO:0006261 ! microscale thermophoresis evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion +intersection_of: ECO:0006261 ! microscale thermophoresis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006305 +name: native protein gel electrophoresis evidence used in manual assertion +def: "A type of native protein gel electrophoresis evidence that is used in a manual assertion." [ECO:SN] +synonym: "BN-PAGE" NARROW [] +synonym: "CN-PAGE" NARROW [] +synonym: "native gel" EXACT [] +synonym: "native PAGE" NARROW [] +synonym: "QPNC-PAGE" NARROW [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0006262 ! native protein gel electrophoresis evidence +intersection_of: ECO:0006262 ! native protein gel electrophoresis evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006306 +name: native protein gel electrophoresis evidence used in automatic assertion +def: "A type of native protein gel electrophoresis evidence that is used in an automatic assertion." [ECO:SN] +synonym: "BN-PAGE" NARROW [] +synonym: "CN-PAGE" NARROW [] +synonym: "native gel" EXACT [] +synonym: "native PAGE" NARROW [] +synonym: "QPNC-PAGE" NARROW [] +is_a: ECO:0006262 ! native protein gel electrophoresis evidence +is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion +intersection_of: ECO:0006262 ! native protein gel electrophoresis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006307 +name: turbidity measurement evidence used in manual assertion +def: "A type of turbidity measurement evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0006263 ! turbidity measurement evidence +intersection_of: ECO:0006263 ! turbidity measurement evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006308 +name: turbidity measurement evidence used in automatic assertion +def: "A type of turbidity measurement evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006263 ! turbidity measurement evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0006263 ! turbidity measurement evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006309 +name: competitive binding evidence used in manual assertion +def: "A type of competitive binding evidence that is used in a manual assertion." [ECO:SN] +synonym: "competition assay" EXACT [] +is_a: ECO:0006075 {is_inferred="true"} ! affinity evidence used in manual assertion +is_a: ECO:0006264 ! competitive binding evidence +intersection_of: ECO:0006264 ! competitive binding evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006310 +name: competitive binding evidence used in automatic assertion +def: "A type of competitive binding evidence that is used in an automatic assertion." [ECO:SN] +synonym: "competition assay" EXACT [] +is_a: ECO:0006264 ! competitive binding evidence +is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion +intersection_of: ECO:0006264 ! competitive binding evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006311 +name: protein unfolding evidence used in manual assertion +def: "A type of protein unfolding evidence that is used in a manual assertion." [ECO:SN] +synonym: "protein denaturation assay" EXACT [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0006265 ! protein unfolding evidence +intersection_of: ECO:0006265 ! protein unfolding evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006312 +name: protein unfolding evidence used in automatic assertion +def: "A type of protein unfolding evidence that is used in an automatic assertion." [ECO:SN] +synonym: "protein denaturation assay" EXACT [] +is_a: ECO:0006265 ! protein unfolding evidence +is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion +intersection_of: ECO:0006265 ! protein unfolding evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006313 +name: urea-induced protein unfolding evidence used in manual assertion +def: "A type of urea-induced protein unfolding evidence that is used in a manual assertion." [ECO:SN] +synonym: "chemical denaturation" BROAD [] +synonym: "chemical unfolding" BROAD [] +is_a: ECO:0006266 ! urea-induced protein unfolding evidence +is_a: ECO:0006311 {is_inferred="true"} ! protein unfolding evidence used in manual assertion +intersection_of: ECO:0006266 ! urea-induced protein unfolding evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006314 +name: urea-induced protein unfolding evidence used in automatic assertion +def: "A type of urea-induced protein unfolding evidence that is used in an automatic assertion." [ECO:SN] +synonym: "chemical denaturation" BROAD [] +synonym: "chemical unfolding" BROAD [] +is_a: ECO:0006266 ! urea-induced protein unfolding evidence +is_a: ECO:0006312 {is_inferred="true"} ! protein unfolding evidence used in automatic assertion +intersection_of: ECO:0006266 ! urea-induced protein unfolding evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006315 +name: pH-induced protein unfolding evidence used in manual assertion +def: "A type of pH-induced protein unfolding evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006267 ! pH-induced protein unfolding evidence +is_a: ECO:0006311 {is_inferred="true"} ! protein unfolding evidence used in manual assertion +intersection_of: ECO:0006267 ! pH-induced protein unfolding evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006316 +name: pH-induced protein unfolding evidence used in automatic assertion +def: "A type of pH-induced protein unfolding evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006267 ! pH-induced protein unfolding evidence +is_a: ECO:0006312 {is_inferred="true"} ! protein unfolding evidence used in automatic assertion +intersection_of: ECO:0006267 ! pH-induced protein unfolding evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006317 +name: temperature-induced protein unfolding evidence used in manual assertion +def: "A type of temperature-induced protein unfolding evidence that is used in a manual assertion." [ECO:SN] +synonym: "thermal dentauration" EXACT [] +synonym: "thermal unfolding" EXACT [] +is_a: ECO:0006268 ! temperature-induced protein unfolding evidence +is_a: ECO:0006311 {is_inferred="true"} ! protein unfolding evidence used in manual assertion +intersection_of: ECO:0006268 ! temperature-induced protein unfolding evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006318 +name: temperature-induced protein unfolding evidence used in automatic assertion +def: "A type of temperature-induced protein unfolding evidence that is used in an automatic assertion." [ECO:SN] +synonym: "thermal dentauration" EXACT [] +synonym: "thermal unfolding" EXACT [] +is_a: ECO:0006268 ! temperature-induced protein unfolding evidence +is_a: ECO:0006312 {is_inferred="true"} ! protein unfolding evidence used in automatic assertion +intersection_of: ECO:0006268 ! temperature-induced protein unfolding evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006319 +name: cell aggregation evidence used in manual assertion +def: "A type of cell aggregation evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0006269 ! cell aggregation evidence +intersection_of: ECO:0006269 ! cell aggregation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006320 +name: cell aggregation evidence used in automatic assertion +def: "A type of cell aggregation evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006269 ! cell aggregation evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0006269 ! cell aggregation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006321 +name: RNA-protein binding evidence used in manual assertion +def: "A type of RNA-protein binding evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion +is_a: ECO:0006083 {is_inferred="true"} ! nucleic acid binding evidence used in manual assertion +is_a: ECO:0006270 ! RNA-protein binding evidence +intersection_of: ECO:0006270 ! RNA-protein binding evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006322 +name: RNA-protein binding evidence used in automatic assertion +def: "A type of RNA-protein binding evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006270 ! RNA-protein binding evidence +is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion +is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion +intersection_of: ECO:0006270 ! RNA-protein binding evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006323 +name: fluorescence microscopy evidence used in manual assertion +def: "A type of fluorescence microscopy evidence that is used in a manual assertion." [ECO:SN] +is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion +is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion +is_a: ECO:0006271 ! fluorescence microscopy evidence +intersection_of: ECO:0006271 ! fluorescence microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006324 +name: fluorescence microscopy evidence used in automatic assertion +def: "A type of fluorescence microscopy evidence that is used in an automatic assertion." [ECO:SN] +is_a: ECO:0006271 ! fluorescence microscopy evidence +is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion +is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion +intersection_of: ECO:0006271 ! fluorescence microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006325 +name: viscosity measurement evidence used in manual assertion +def: "A type of viscosity measurement evidence that is used in a manual assertion." [ECO:SN] +synonym: "viscometry" EXACT [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0006272 ! viscosity measurement evidence +intersection_of: ECO:0006272 ! viscosity measurement evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006326 +name: viscosity measurement evidence used in automatic assertion +def: "A type of viscosity measurement evidence that is used in an automatic assertion." [ECO:SN] +synonym: "viscometry" EXACT [] +is_a: ECO:0006272 ! viscosity measurement evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0006272 ! viscosity measurement evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006327 +name: dynamic fluorescence quenching evidence used in manual assertion +def: "A type of dynamic fluorescence quenching evidence that is used in a manual assertion." [ECO:SN] +synonym: "Dexter" NARROW [] +synonym: "exciplex" NARROW [] +synonym: "FRET" NARROW [] +synonym: "quenching" BROAD [] +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion +is_a: ECO:0006273 ! dynamic fluorescence quenching evidence +intersection_of: ECO:0006273 ! dynamic fluorescence quenching evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006328 +name: dynamic fluorescence quenching evidence used in automatic assertion +def: "A type of dynamic fluorescence quenching evidence that is used in an automatic assertion." [ECO:SN] +synonym: "Dexter" NARROW [] +synonym: "exciplex" NARROW [] +synonym: "FRET" NARROW [] +synonym: "quenching" BROAD [] +is_a: ECO:0006273 ! dynamic fluorescence quenching evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion +intersection_of: ECO:0006273 ! dynamic fluorescence quenching evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006329 +name: static fluorescence quenching evidence used in manual assertion +def: "A type of static fluorescence quenching evidence that is used in a manual assertion." [ECO:SN] +synonym: "quenching" BROAD [] +is_a: ECO:0000353 {is_inferred="true"} ! physical interaction evidence used in manual assertion +is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion +is_a: ECO:0006274 ! static fluorescence quenching evidence +intersection_of: ECO:0006274 ! static fluorescence quenching evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0006330 +name: static fluorescence quenching evidence used in automatic assertion +def: "A type of static fluorescence quenching evidence that is used in an automatic assertion." [ECO:SN] +synonym: "quenching" BROAD [] +is_a: ECO:0006274 ! static fluorescence quenching evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion +intersection_of: ECO:0006274 ! static fluorescence quenching evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: IAO:0000234 DisProt:BalintMeszaros xsd:string +created_by: snadendla + +[Term] +id: ECO:0007000 +name: high throughput mutant phenotypic evidence +def: "A type of high throughput evidence derived from high throughput analysis of differences in alleles of a corresponding gene." [GO:IMP] +synonym: "high throughput mutant phenotype evidence" EXACT [] +is_a: ECO:0000015 ! mutant phenotype evidence +is_a: ECO:0006055 ! high throughput evidence +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007001 +name: high throughput mutant phenotypic evidence used in manual assertion +def: "A type of high throughput mutant phenotypic evidence that is used in a manual assertion." [GO:IMP] +comment: When using the HMP evidence code, the guidelines for IMP should be adhered to (http://geneontology.org/page/imp-inferred-mutant-phenotype) +synonym: "high throughput mutant phenotype evidence used in manual assertion" EXACT [] +synonym: "HMP" EXACT [GOECO:HMP] +synonym: "inferred from high throughput mutant phenotype" EXACT [GOECO:HMP] +xref: GOECO:HMP "inferred from high throughput mutant phenotype" +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0006056 {is_inferred="true"} ! high throughput evidence used in manual assertion +is_a: ECO:0007000 ! high throughput mutant phenotypic evidence +intersection_of: ECO:0007000 ! high throughput mutant phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "HMP" xsd:string {comment="Default"} +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007002 +name: high throughput genetic interaction phenotypic evidence +def: "A type of high throughput evidence resulting from the high throughput analysis of the effect that a given gene has on another gene or genes, and products." [PMID:11822023] +synonym: "high throughput genetic interaction evidence" EXACT [] +is_a: ECO:0000011 ! genetic interaction evidence +is_a: ECO:0006055 ! high throughput evidence +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007003 +name: high throughput genetic interaction phenotypic evidence used in manual assertion +def: "A type of high throughput genetic interaction phenotypic evidence that is used in a manual assertion." [PMID:11822023] +comment: When using the HGI evidence code, the guidelines for IGI should be adhered to (http://geneontology.org/page/igi-inferred-genetic-interaction) +synonym: "HGI" EXACT [GOECO:HGI] +synonym: "high throughput genetic interaction evidence used in manual assertion" EXACT [] +synonym: "inferred from high throughput genetic interaction" EXACT [GOECO:HGI] +xref: GOECO:HGI "inferred from high throughput genetic interaction" +is_a: ECO:0000316 {is_inferred="true"} ! genetic interaction evidence used in manual assertion +is_a: ECO:0006056 {is_inferred="true"} ! high throughput evidence used in manual assertion +is_a: ECO:0007002 ! high throughput genetic interaction phenotypic evidence +intersection_of: ECO:0007002 ! high throughput genetic interaction phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "HGI" xsd:string {comment="Default"} +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007004 +name: high throughput direct assay evidence +def: "A type of high throughput evidence derived from the high throughput direct measurement of some aspect of a biological feature." [ECO:MCC] +is_a: ECO:0000002 ! direct assay evidence +is_a: ECO:0006055 ! high throughput evidence +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007005 +name: high throughput direct assay evidence used in manual assertion +def: "A type of high throughput evidence that is used in a manual assertion derived from the high throughput direct measurement of some aspect of a biological feature." [ECO:MCC] +comment: When using the HDA evidence code, the guidelines for IDA should be adhered to (http://geneontology.org/page/ida-inferred-direct-assay) +synonym: "HDA" EXACT [GOECO:HDA] +synonym: "inferred from high throughput direct assay" EXACT [GOECO:HDA] +xref: GOECO:HDA "inferred from high throughput direct assay" +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0006056 {is_inferred="true"} ! high throughput evidence used in manual assertion +is_a: ECO:0007004 ! high throughput direct assay evidence +intersection_of: ECO:0007004 ! high throughput direct assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "HDA" xsd:string {comment="Default"} +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007006 +name: high throughput expression pattern evidence +def: "A type of high throughput evidence derived from the high throughput characterization of gene expression." [GO:IEP] +is_a: ECO:0000008 ! expression pattern evidence +is_a: ECO:0006055 ! high throughput evidence +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007007 +name: high throughput expression pattern evidence used in manual assertion +def: "A type of high throughput evidence that is used in a manual assertion derived from the high throughput characterization of gene expression." [GO:IEP] +comment: When using the HEP evidence code, the guidelines for EXP should be adhered to (http://geneontology.org/page/iep-inferred-expression-pattern) +synonym: "HEP" EXACT [GOECO:HEP] +synonym: "inferred from high throughput expression pattern" EXACT [GOECO:HEP] +xref: GOECO:HEP "inferred from high throughput expression pattern" +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0006056 {is_inferred="true"} ! high throughput evidence used in manual assertion +is_a: ECO:0007006 ! high throughput expression pattern evidence +intersection_of: ECO:0007006 ! high throughput expression pattern evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "HEP" xsd:string {comment="Default"} +property_value: IAO:0000234 "GOC" xsd:string +created_by: rctauber +creation_date: 2017-10-05T07:39:47Z + +[Term] +id: ECO:0007008 +name: radioligand binding assay evidence +def: "A type of protein binding evidence in which radioactive ligands are used to measure receptor-ligand interactions, such as in determining selectivity for a particular ligand." [PMID:27471749] +synonym: "competitive binding assay evidence" NARROW [] +synonym: "radioactive ligand binding assay evidence" EXACT [] +synonym: "radiometric ligand-binding assay evidence" EXACT [] +is_a: ECO:0000024 ! protein binding evidence +property_value: IAO:0000234 "fjungo" xsd:string +created_by: rctauber +creation_date: 2017-12-15T10:46:19Z + +[Term] +id: ECO:0007009 +name: radioligand binding assay evidence used in manual assertion +def: "A type of radioligand binding assay evidence that is used in a manual assertion." [ECO:RCT] +synonym: "competitive binding assay evidence" RELATED [] +synonym: "radioactive ligand binding assay evidence" BROAD [] +is_a: ECO:0006076 {is_inferred="true"} ! protein binding evidence used in manual assertion +is_a: ECO:0007008 ! radioligand binding assay evidence +intersection_of: ECO:0007008 ! radioligand binding assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2017-12-15T10:48:44Z + +[Term] +id: ECO:0007011 +name: combinatorial experimental and author inference evidence +def: "A type of combinatorial evidence in which the author draws a conclusion based on a combination of prior scientific background knowledge and evidence from one or more experiments." [ECO:RCT] +synonym: "combinatorial evidence from author knowledge and experimental evidence" EXACT [] +is_a: ECO:0000212 {is_inferred="true"} ! combinatorial evidence +intersection_of: ECO:0000212 ! combinatorial evidence +intersection_of: BFO:0000051 ECO:0000006 ! experimental evidence +intersection_of: BFO:0000051 ECO:0006185 ! author inference +property_value: IAO:0000234 "sbello" xsd:string +created_by: rctauber +creation_date: 2018-02-15T11:17:11Z + +[Term] +id: ECO:0007012 +name: combinatorial experimental and curator inference evidence +def: "A type of combinatorial evidence in which the curator draws a conclusion based on a combination of prior scientific background knowledge and evidence from one or more experiments." [ECO:RCT] +synonym: "combinatorial evidence from curator knowledge and experimental evidence" EXACT [] +is_a: ECO:0000212 {is_inferred="true"} ! combinatorial evidence +intersection_of: ECO:0000212 ! combinatorial evidence +intersection_of: BFO:0000051 ECO:0000006 ! experimental evidence +intersection_of: BFO:0000051 ECO:0000205 ! curator inference +property_value: IAO:0000234 "ZFIN" xsd:string +created_by: rctauber +creation_date: 2018-02-15T11:17:11Z + +[Term] +id: ECO:0007013 +name: combinatorial experimental and author inference evidence used in manual assertion +def: "A type of combinatorial evidence from author knowledge and experimental evidence that is used in a manual assertion." [ECO:RCT] +synonym: "combinatorial evidence from author knowledge and experimental evidence used in manual assertion" EXACT [] +is_a: ECO:0000244 {is_inferred="true"} ! combinatorial evidence used in manual assertion +is_a: ECO:0007011 ! combinatorial experimental and author inference evidence +intersection_of: ECO:0007011 ! combinatorial experimental and author inference evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-02-15T11:22:10Z + +[Term] +id: ECO:0007014 +name: combinatorial experimental and curator inference evidence used in manual assertion +def: "A type of combinatorial evidence from curator knowledge and experimental evidence that is used in a manual assertion." [ECO:RCT] +synonym: "combinatorial evidence from curator knowledge and experimental evidence used in manual assertion" EXACT [] +is_a: ECO:0000244 {is_inferred="true"} ! combinatorial evidence used in manual assertion +is_a: ECO:0007012 ! combinatorial experimental and curator inference evidence +intersection_of: ECO:0007012 ! combinatorial experimental and curator inference evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-02-15T11:22:10Z + +[Term] +id: ECO:0007015 +name: voltammetry evidence +def: "A type of substance quantification evidence where an electrical gradient potential is applied to analyze an analyte of interest, resulting in a measurement amount of electrical current across the potential range applied." [PMID:28127962] +is_a: ECO:0000335 ! substance quantification evidence +property_value: IAO:0000234 "SynGO" xsd:string +created_by: rctauber +creation_date: 2018-02-28T12:11:00Z + +[Term] +id: ECO:0007016 +name: voltammetry evidence used in manual assertion +def: "A type of voltammetry evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0007015 ! voltammetry evidence +intersection_of: ECO:0007015 ! voltammetry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-02-28T12:12:34Z + +[Term] +id: ECO:0007017 +name: photoconversion evidence +def: "A type of fluorescence evidence where a molecule tagged with a fluorescent protein is exposed to ultraviolet or blue light to shift the spectral emission properties for visualization." [ECO:RCT, PMID:28574633] +is_a: ECO:0001115 ! fluorescence evidence +property_value: IAO:0000234 "SynGO" xsd:string +created_by: rctauber +creation_date: 2018-03-02T09:01:00Z + +[Term] +id: ECO:0007018 +name: photoconversion evidence used in manual assertion +def: "A type of photoconversion evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion +is_a: ECO:0007017 ! photoconversion evidence +intersection_of: ECO:0007017 ! photoconversion evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-03-02T09:05:04Z + +[Term] +id: ECO:0007019 +name: agglutination test evidence +def: "A type of immunological assay evidence that involves observation of visible clumping of antibody and antigen into a complex." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC380037/] +synonym: "agglutination assay evidence" EXACT [] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007020 +name: agglutination test evidence used in manual assertion +def: "A type of agglutination test evidence that is used in a manual assertion." [ECO:RCT] +synonym: "agglutination assay evidence" BROAD [] +is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion +is_a: ECO:0007019 ! agglutination test evidence +intersection_of: ECO:0007019 ! agglutination test evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007021 +name: slide agglutination test evidence +def: "A type of agglutination test evidence that involves observaiton of visible clumping of antibody and antigen into a complex on a slide." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC380037/] +is_a: ECO:0007019 ! agglutination test evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007022 +name: slide agglutination test evidence used in manual assertion +def: "A type of slide agglutination test evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0007020 {is_inferred="true"} ! agglutination test evidence used in manual assertion +is_a: ECO:0007021 ! slide agglutination test evidence +intersection_of: ECO:0007021 ! slide agglutination test evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007023 +name: direct Coombs test evidence +def: "A type of agglutination test evidence that results in detection of nonagglutinating antibodies or complement proteins on red blood cells in vivo." [URL:https\://courses.lumenlearning.com/microbiology/chapter/agglutination-assays/] +synonym: "direct antihuman globulin test (DAT)" EXACT [] +is_a: ECO:0007019 ! agglutination test evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007024 +name: direct Coombs test evidence used in manual assertion +def: "A type of direct Coombs test evidence that is used in a manual assertion." [ECO:RCT] +synonym: "direct antihuman globulin test (DAT)" BROAD [] +is_a: ECO:0007020 {is_inferred="true"} ! agglutination test evidence used in manual assertion +is_a: ECO:0007023 ! direct Coombs test evidence +intersection_of: ECO:0007023 ! direct Coombs test evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007025 +name: indirect Coombs test evidence +def: "A type of agglutination test evidence that results in screening of antibodies against red blood cell antigens (other than the A and B antigens) that are unbound in a patient's serum in vitro." [URL:https\://courses.lumenlearning.com/microbiology/chapter/agglutination-assays/] +synonym: "indirect antiglobulin test (IAT)" EXACT [] +is_a: ECO:0007019 ! agglutination test evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007026 +name: indirect Coombs test evidence used in manual assertion +def: "A type of indirect Coombs test evidence that is used in a manual assertion." [ECO:RCT] +synonym: "indirect antiglobulin test (IAT)" BROAD [] +is_a: ECO:0007020 {is_inferred="true"} ! agglutination test evidence used in manual assertion +is_a: ECO:0007025 ! indirect Coombs test evidence +intersection_of: ECO:0007025 ! indirect Coombs test evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007027 +name: direct hemagglutination assay evidence +def: "A type of agglutination test evidence where some bacteria and viruses cross-link red blood cells and clump together." [URL:https\://courses.lumenlearning.com/microbiology/chapter/agglutination-assays/] +is_a: ECO:0007019 ! agglutination test evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007028 +name: direct hemagglutination assay evidence used in manual assertion +def: "A type of direct hemagglutination assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0007020 {is_inferred="true"} ! agglutination test evidence used in manual assertion +is_a: ECO:0007027 ! direct hemagglutination assay evidence +intersection_of: ECO:0007027 ! direct hemagglutination assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007029 +name: viral hemagglutination inhibition assay evidence +def: "A type of agglutination test evidence where antiviral antibodies in a patient's serum or in a lab-produced antiserum neutralize the virus and block it from agglutinating the red blood cells." [URL:https\://courses.lumenlearning.com/microbiology/chapter/agglutination-assays/] +is_a: ECO:0007019 ! agglutination test evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007030 +name: viral hemagglutination inhibition assay evidence used in manual assertion +def: "A type of viral hemagglutination inhibition assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0007020 {is_inferred="true"} ! agglutination test evidence used in manual assertion +is_a: ECO:0007029 ! viral hemagglutination inhibition assay evidence +intersection_of: ECO:0007029 ! viral hemagglutination inhibition assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007031 +name: compement fixation assay evidence +def: "A type of immunological assay evidence that results in the detection of presence of specific antibodies in the patient's serum based on the use of complement, a biologically labile serum factor." [URL:http\://laboratoryinfo.com/complement-fixation-test/] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007032 +name: compement fixation assay evidence used in manual assertion +def: "A type of compement fixation assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion +is_a: ECO:0007031 ! compement fixation assay evidence +intersection_of: ECO:0007031 ! compement fixation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007033 +name: neutralization test assay evidence +def: "A type of immunogical assay evidence that results in detection of specific neutralizing antibodies in a sample." [PMID:24899440, url:http\://www.creative-biolabs.com/drug-discovery/therapeutics/neutralization-assay.htm?gclid=Cj0KEQjwqvvLBRDIt-D7q7iqiOcBEiQAxi68ERRz00a0x9sWSKSLDu7d5-kMi4AdiLNdUq1mXzePdywaArli8P8HAQ)] +is_a: ECO:0000040 ! immunological assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007034 +name: neutralization test assay evidence used in manual assertion +def: "A type of neutralization test assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006078 {is_inferred="true"} ! immunological assay evidence used in manual assertion +is_a: ECO:0007033 ! neutralization test assay evidence +intersection_of: ECO:0007033 ! neutralization test assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007035 +name: copper transport assay evidence +def: "A type of transport assay evidence where the rate of copper transport is estimated." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689948/] +is_a: ECO:0000134 ! transport assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007036 +name: copper transport assay evidence used in manual assertion +def: "A type of copper transport assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0007035 ! copper transport assay evidence +intersection_of: ECO:0007035 ! copper transport assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007037 +name: 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence +def: "A type of staining evidence that involves the use of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride to evaluate the respiratory activity of cells." [PMID:1622256] +synonym: "CTC staining" EXACT [] +is_a: ECO:0005019 ! staining evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007038 +name: 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence used in manual assertion +def: "A type of 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence that is used in a manual assertion." [ECO:RCT] +synonym: "CTC staining" BROAD [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0007037 ! 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence +intersection_of: ECO:0007037 ! 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007039 +name: plaque assay evidence +def: "A type of cell growth assay evidence resulting in direct quantification of infectious virons and antiviral substances through the counting of discrete plaques (infectious units and cellular dead zones) in cell culture." [PMID:25407402] +is_a: ECO:0001563 ! cell growth assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007040 +name: plaque assay evidence used in manual assertion +def: "A type of plaque assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0007039 ! plaque assay evidence +intersection_of: ECO:0007039 ! plaque assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007041 +name: epifluorescence microscopy evidence +def: "A type of fluorescence microscopy evidence where the light source is mounted above the specimen and the excitation light passes through the microscope objective lens on its way toward the specimen." [URL:http\://www.rsc.org/publishing/journals/prospect/ontology.asp?id=CMO\:0001096|URL\:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713126/] +is_a: ECO:0006271 ! fluorescence microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007042 +name: epifluorescence microscopy evidence used in manual assertion +def: "A type of epifluorescence microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006323 {is_inferred="true"} ! fluorescence microscopy evidence used in manual assertion +is_a: ECO:0007041 ! epifluorescence microscopy evidence +intersection_of: ECO:0007041 ! epifluorescence microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007043 +name: transmission electron microscopy evidence +def: "A type of electron microscopy evidence where a beam of electrons are transmitted through an ultra thin specimen forming an image which is magnified and focused onto an imaging device, such as a fluorescent screen, on a layer of photographic film, or to be detected by a sensor such as a CCD camera." [ERO:0000329|URL\:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907272/] +synonym: "conventional transmission electron microscopy(CTEM)" EXACT [] +synonym: "TEM" EXACT [] +is_a: ECO:0005033 ! electron microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007044 +name: transmission electron microscopy evidence used in manual assertion +def: "A type of transmission electron microscopy evidence that is used in a manual assertion." [ECO:RCT] +synonym: "conventional transmission electron microscopy(CTEM)" BROAD [] +synonym: "TEM" BROAD [] +is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion +is_a: ECO:0007043 ! transmission electron microscopy evidence +intersection_of: ECO:0007043 ! transmission electron microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007045 +name: scanning electron microscopy evidence +def: "A type of electron microscopy evidence where a focused beam of high-energy electrons is used to generate a variety of signals at the surface of solid specimens." [URL:https\://serc.carleton.edu/research_education/geochemsheets/techniques/SEM.html] +is_a: ECO:0005033 ! electron microscopy evidence +property_value: IAO:0000112 "With a general growth of the recombinant mycobacterial strains resulting in minimal change, the cell morphology was further examined using the scanning electron microscopy (SEM) technique. As shown in Fig. 5B, the cell lengthened when 20 ng/mL tetracycline was added to the medium to induce expression of the antisense mtrA mRNA (right panel)." xsd:string {xref="PMID:20843371"} +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007046 +name: scanning electron microscopy evidence used in manual assertion +def: "A type of scanning electron microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006003 {is_inferred="true"} ! electron microscopy evidence used in manual assertion +is_a: ECO:0007045 ! scanning electron microscopy evidence +intersection_of: ECO:0007045 ! scanning electron microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007047 +name: time-lapsed microscopy evidence +def: "A type of microscopy evidence where a serial images are taken at regular time points to capture the dynamics of what is being observed." [URL:https\://www.nature.com/subjects/time-lapse-imaging] +is_a: ECO:0001098 ! microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007048 +name: time-lapsed microscopy evidence used in manual assertion +def: "A type of time-lapsed microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion +is_a: ECO:0007047 ! time-lapsed microscopy evidence +intersection_of: ECO:0007047 ! time-lapsed microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007049 +name: phase contrast microscopy evidence +def: "A type of microscopy evidence where a transparent specimen is illuminated with visible light and small phase shifts in the light passing through the specimen are used to produce an image." [CHMO:0000110] +is_a: ECO:0001098 ! microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007050 +name: phase contrast microscopy evidence used in manual assertion +def: "A type of phase contrast microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion +is_a: ECO:0007049 ! phase contrast microscopy evidence +intersection_of: ECO:0007049 ! phase contrast microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007051 +name: transmitted light brightfied mircoscopy evidence +def: "A type of microscopy evidence where a sample is illuminated with transmitted white light from below and observed from above." [BAO:0000457] +is_a: ECO:0001098 ! microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007052 +name: transmitted light brightfied mircoscopy evidence used in manual assertion +def: "A type of transmitted light brightfied mircoscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion +is_a: ECO:0007051 ! transmitted light brightfied mircoscopy evidence +intersection_of: ECO:0007051 ! transmitted light brightfied mircoscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007053 +name: koehler illumination microscopy evidence +def: "A type of microscopy evidence that results in a bright image without glare and minimum heating of the specimen by employing both field and an aperture iris diaphragm for illumination." [URL:http\://www.gonda.ucla.edu/bri_core/kohler.htm] +is_a: ECO:0001098 ! microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007054 +name: koehler illumination microscopy evidence used in manual assertion +def: "A type of koehler illumination microscopy evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion +is_a: ECO:0007053 ! koehler illumination microscopy evidence +intersection_of: ECO:0007053 ! koehler illumination microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007055 +name: differential interference contrast microscopy evidence +def: "A type of microscopy evidence that results in visualization of living cells and transparent specimens by taking advantage of differences in the light refraction of different parts of the specimen." [URL:http\://www.microscopemaster.com/differential-interference-contrast.html] +synonym: "DIC microscopy" EXACT [] +synonym: "NIC microscopy" EXACT [] +synonym: "Nomarski interference contrast microscopy" EXACT [] +synonym: "Nomarski microscopy" EXACT [] +is_a: ECO:0001098 ! microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007056 +name: differential interference contrast microscopy evidence used in manual assertion +def: "A type of differential interference contrast microscopy evidence that is used in a manual assertion." [ECO:RCT] +synonym: "DIC microscopy" BROAD [] +synonym: "NIC microscopy" BROAD [] +synonym: "Nomarski interference contrast microscopy" BROAD [] +synonym: "Nomarski microscopy" BROAD [] +is_a: ECO:0001232 {is_inferred="true"} ! microscopy evidence used in manual assertion +is_a: ECO:0007055 ! differential interference contrast microscopy evidence +intersection_of: ECO:0007055 ! differential interference contrast microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007057 +name: extended field laser confocal microscopy evidence +def: "A type of confocal microscopy evidence that results in creation of a continuous confocal multi-colour mosaic from thousands of individually captured images." [PMID:18627634] +synonym: "EFLCM" EXACT [] +is_a: ECO:0005587 ! confocal microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007058 +name: extended field laser confocal microscopy evidence used in manual assertion +def: "A type of extended field laser confocal microscopy evidence that is used in a manual assertion." [ECO:RCT] +synonym: "EFLCM" BROAD [] +is_a: ECO:0005589 {is_inferred="true"} ! confocal microscopy evidence used in manual assertion +is_a: ECO:0007057 ! extended field laser confocal microscopy evidence +intersection_of: ECO:0007057 ! extended field laser confocal microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007059 +name: confocal laser scanning microscopy evidence +def: "A type of confocal microscopy evidence that results in an image that is built up pixel-by-pixel by collecting the emitted photons from the fluorophores in the sample by passing a laser beam through a light source aperture which is then focused by an objective lens into a small area on the surface of the sample.\n" [URL:http\://bitesizebio.com/19958/what-is-confocal-laser-scanning-microscopy/] +synonym: "CLSM" EXACT [] +synonym: "confocal laser scanning fluorescence microscopy" EXACT [] +synonym: "confocal-laser scanning microscopy" EXACT [] +synonym: "fluorescence confocal scanning laser microscopy" EXACT [] +synonym: "LSCM" EXACT [] +synonym: "scanning confocal fluorescence microscopy" EXACT [] +is_a: ECO:0005587 ! confocal microscopy evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007060 +name: confocal laser scanning microscopy evidence used in manual assertion +def: "A type of confocal laser scanning microscopy evidence that is used in a manual assertion." [ECO:RCT] +synonym: "CLSM" BROAD [] +synonym: "confocal laser scanning fluorescence microscopy" BROAD [] +synonym: "confocal-laser scanning microscopy" BROAD [] +synonym: "fluorescence confocal scanning laser microscopy" BROAD [] +synonym: "LSCM" BROAD [] +synonym: "scanning confocal fluorescence microscopy" BROAD [] +is_a: ECO:0005589 {is_inferred="true"} ! confocal microscopy evidence used in manual assertion +is_a: ECO:0007059 ! confocal laser scanning microscopy evidence +intersection_of: ECO:0007059 ! confocal laser scanning microscopy evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007061 +name: light scattering evidence +def: "A type of structure determination evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight, diffusion and interaction strength by illuminating the sample with a laser beam and the scattered intensity is probed at a certain angle by a detector." [PMID:9013660, url:http\://www.lsinstruments.ch/technology/dynamic_light_scattering_dls/, url:http\://www.soft-matter.uni-tuebingen.de/index.html?dls.html] +is_a: ECO:0005031 ! structure determination evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007062 +name: light scattering evidence used in manual assertion +def: "A type of light scattering evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0007061 ! light scattering evidence +intersection_of: ECO:0007061 ! light scattering evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007063 +name: dynamic light scattering assay evidence +def: "A type of light scattering evidence derived by analyzing the fluctuations in the internsity of the scattered light by the particles in motion." [PMID:9013660, url:http\://www.soft-matter.uni-tuebingen.de/index.html?dls.html] +synonym: "Photon Correlation Spectroscopy" EXACT [] +synonym: "Quasi-Elastic Light Scattering" EXACT [] +is_a: ECO:0007061 ! light scattering evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007064 +name: dynamic light scattering assay evidence used in manual assertion +def: "A type of dynamic light scattering assay evidence that is used in a manual assertion." [ECO:RCT] +synonym: "Photon Correlation Spectroscopy" BROAD [] +synonym: "Quasi-Elastic Light Scattering" BROAD [] +is_a: ECO:0007062 {is_inferred="true"} ! light scattering evidence used in manual assertion +is_a: ECO:0007063 ! dynamic light scattering assay evidence +intersection_of: ECO:0007063 ! dynamic light scattering assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007065 +name: static light scattering assay evidence +def: "A type of light scattering evidence derived by measuring the average intensity of scattered light by the particles at multiple angles." [PMID:9013660] +synonym: "Rayleigh scattering" EXACT [] +is_a: ECO:0007061 ! light scattering evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007066 +name: static light scattering assay evidence used in manual assertion +def: "A type of static light scattering assay evidence that is used in a manual assertion." [ECO:RCT] +synonym: "Rayleigh scattering" BROAD [] +is_a: ECO:0007062 {is_inferred="true"} ! light scattering evidence used in manual assertion +is_a: ECO:0007065 ! static light scattering assay evidence +intersection_of: ECO:0007065 ! static light scattering assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007067 +name: colony papillation assay phenotypic evidence +def: "A type of colony morphology phenotypic evidence resulting from the formation of papillae (microcolonies) that protrude outwards from the main colony by mutant cells." [PMID:27447898] +synonym: "colony papillation assay evidence" EXACT [] +is_a: ECO:0007077 ! colony morphology phenotypic evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007068 +name: colony papillation assay phenotypic evidence used in manual assertion +def: "A type of colony papillation assay phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "colony papillation assay evidence used in manual assertion" EXACT [] +is_a: ECO:0007067 ! colony papillation assay phenotypic evidence +is_a: ECO:0007078 {is_inferred="true"} ! colony morphology phenotypic evidence used in manual assertion +intersection_of: ECO:0007067 ! colony papillation assay phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007069 +name: crystal violet staining evidence +def: "A type of staining evidence that is derived from the use of gentian violet as a general biological stain and an acid-base indicator." [BAO:0002468] +synonym: "gentian violet" EXACT [] +synonym: "hexamethyl pararosaniline chloride" EXACT [] +synonym: "methyl violet 10B" EXACT [] +is_a: ECO:0005019 ! staining evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007070 +name: crystal violet staining evidence used in manual assertion +def: "A type of crystal violet staining evidence that is used in a manual assertion." [ECO:RCT] +synonym: "gentian violet" BROAD [] +synonym: "hexamethyl pararosaniline chloride" BROAD [] +synonym: "methyl violet 10B" BROAD [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0007069 ! crystal violet staining evidence +intersection_of: ECO:0007069 ! crystal violet staining evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007071 +name: flow cell biofilm assay evidence +def: "A type of biofilm formation assay evidence derived from in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3438488/] +is_a: ECO:0006020 ! biofilm formation assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007072 +name: flow cell biofilm assay evidence used in manual assertion +def: "A type of flow cell biofilm assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006021 {is_inferred="true"} ! biofilm formation assay evidence used in manual assertion +is_a: ECO:0007071 ! flow cell biofilm assay evidence +intersection_of: ECO:0007071 ! flow cell biofilm assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007073 +name: bacterial 2-hybrid assay evidence +def: "A type of bait-prey hybrid interaction evidence that involves the detection of protein-protein interaction by fusing the protein target (the \"bait\") to RNA polymerase and fusing protein or peptide library to be analyzed (the \"prey\") to DNA-binding domain." [URL:http\://www.pnas.org/content/97/13/7382.full.pdf] +is_a: ECO:0000025 ! bait-prey hybrid interaction evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007074 +name: bacterial 2-hybrid assay evidence used in manual assertion +def: "A type of bacterial 2-hybrid assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0006077 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in manual assertion +is_a: ECO:0007073 ! bacterial 2-hybrid assay evidence +intersection_of: ECO:0007073 ! bacterial 2-hybrid assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007075 +name: phenomic profiling assay evidence +def: "A type of experimental phenotypic evidence that results from the comparison of phenotype profiles (the physical and biochemical traits of organisms in particular environments) across multiple strains; that is, a meta-analysis of a set of phenotypic patterns from many tests in response to a series of changing genetic and/or environmental factors." [EDAM:topic\:3298, PMID:2118507] +comment: A phenome is defined as the SET of physical and biochemical traits, or, as the SET of all phenotypes expressed, i.e. a phenome is defined as the sum total of all phenotypic traits. +comment: D.S. notes: I think this term is meant to be used for high throughput experiments where a large number of mutants is screened for their ability to grow in a large number of conditions. The individual mutants can then clustered based on the similarity of their phenotypic profiles-whether they grew or didn't grow across all the conditions. The goal being to try to identify genes whose products function in the same biological pathways based on their having similar phenotypes across multiple conditions. +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007076 +name: phenomic profiling assay evidence used in manual assertion +def: "A type of phenomic profiling assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0007075 ! phenomic profiling assay evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0007075 ! phenomic profiling assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007077 +name: colony morphology phenotypic evidence +def: "A type of experimental phenotypic evidence resulting from studying the physical characteristics of an assemblage of microorganisms growing on a solid surface such as the surface of an agar culture medium." [OMP:0000100] +synonym: "colony morphology evidence" EXACT [] +is_a: ECO:0000059 ! experimental phenotypic evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007078 +name: colony morphology phenotypic evidence used in manual assertion +def: "A type of colony morphology phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "colony morphology evidence used in manual assertion" EXACT [] +is_a: ECO:0007077 ! colony morphology phenotypic evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0007077 ! colony morphology phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007079 +name: colony color phenotypic evidence +def: "A type of colony morphology evidence resulting from studying the colony pigmentation." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671912/] +synonym: "colony color evidence" EXACT [] +synonym: "colony pigmentation" EXACT [] +is_a: ECO:0007077 ! colony morphology phenotypic evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007080 +name: colony color phenotypic evidence used in manual assertion +def: "A type of colony color phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "colony color evidence used in manual assertion" EXACT [] +synonym: "colony pigmentation" BROAD [] +is_a: ECO:0007078 {is_inferred="true"} ! colony morphology phenotypic evidence used in manual assertion +is_a: ECO:0007079 ! colony color phenotypic evidence +intersection_of: ECO:0007079 ! colony color phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007081 +name: colony size phenotypic evidence +def: "A type of colony morphology phenotypic evidence resulting from measuring the area or diameter of the colony." [URL:http\://www.nature.com/nrmicro/journal/v4/n4/full/nrmicro1384.html] +synonym: "colony size evidence" EXACT [] +is_a: ECO:0007077 ! colony morphology phenotypic evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007082 +name: colony size phenotypic evidence used in manual assertion +def: "A type of colony size phenotypic evidence that is used in an manual assertion." [ECO:RCT] +synonym: "colony area phenotype evidence" NARROW [] +synonym: "colony diameter phenotype evidence" NARROW [] +synonym: "colony size evidence used in manual assertion" EXACT [] +is_a: ECO:0007078 {is_inferred="true"} ! colony morphology phenotypic evidence used in manual assertion +is_a: ECO:0007081 ! colony size phenotypic evidence +intersection_of: ECO:0007081 ! colony size phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007083 +name: zone of inhibition evidence +def: "A type of cell growth assay evidence resulting from assessing the sensitivity or resistance of bacteria or fungi to an antimicrobial agent or chemical by measuring the size of the zone of inhibition that results when the test organism is grown on a solid surface in the presence of the antimicrobial agent or chemical." [URL:https\://www.ncbi.nlm.nih.gov/pmc/articles/PMC153338/] +is_a: ECO:0001563 ! cell growth assay evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007084 +name: zone of inhibition evidence used in manual assertion +def: "A type of zone of inhibition evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0007083 ! zone of inhibition evidence +intersection_of: ECO:0007083 ! zone of inhibition evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007085 +name: Etest evidence +def: "A type of zone of inhibition evidence resulting from testing the antimicrobial susceptibility by using predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip." [URL:http\://www.joponline.org/doi/pdf/10.1902/jop.1992.63.7.576] +synonym: "epsilometer test" EXACT [] +is_a: ECO:0007083 ! zone of inhibition evidence +property_value: IAO:0000234 "OMP" xsd:string +created_by: snadendla +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007086 +name: Etest evidence used in manual assertion +def: "A type of Etest evidence that is used in a manual assertion." [ECO:RCT] +synonym: "epsilometer test" BROAD [] +is_a: ECO:0007084 {is_inferred="true"} ! zone of inhibition evidence used in manual assertion +is_a: ECO:0007085 ! Etest evidence +intersection_of: ECO:0007085 ! Etest evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +property_value: IAO:0000234 "OMP" xsd:string +created_by: rctauber +creation_date: 2018-03-14T12:44:11Z + +[Term] +id: ECO:0007087 +name: ribosome profiling evidence +def: "A type of expression pattern evidence in which translation is measured by the positions of ribosomes active in a cell, identified through deep sequencing of ribosome-protected mRNA fragments." [PMID:27015305] +synonym: "ribo-seq evidence" EXACT [] +synonym: "ribosome footprinting evidence" EXACT [] +synonym: "translation profiling" NARROW [] +synonym: "translatome profiling" NARROW [] +is_a: ECO:0000008 ! expression pattern evidence +property_value: IAO:0000234 GOC:VW xsd:string +created_by: rctauber +creation_date: 2018-03-28T10:27:09Z + +[Term] +id: ECO:0007088 +name: ribosome profiling evidence used in manual assertion +def: "A type of ribosome profiling evidence that is used in a manual assertion." [ECO:RCT] +synonym: "ribo-seq evidence" BROAD [] +synonym: "ribosome footprinting evidence" BROAD [] +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +is_a: ECO:0007087 ! ribosome profiling evidence +intersection_of: ECO:0007087 ! ribosome profiling evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2018-03-28T10:33:26Z + +[Term] +id: ECO:0007089 +name: loss-of-function mutant phenotype evidence used in manual assertion +def: "A type of loss-of-function mutant phenotype evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000016 {is_inferred="true"} ! loss-of-function mutant phenotype evidence +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +intersection_of: ECO:0000016 ! loss-of-function mutant phenotype evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007090 +name: structural similarity evidence used in manual assertion +def: "A type of structural similarity evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000027 {is_inferred="true"} ! structural similarity evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +intersection_of: ECO:0000027 ! structural similarity evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007092 +name: gain-of-function mutant phenotypic evidence used in manual assertion +def: "A type of gain-of-function mutant phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "gain-of-function mutant phenotype evidence used in manual assertion" EXACT [] +is_a: ECO:0000042 {is_inferred="true"} ! gain-of-function mutant phenotypic evidence +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +intersection_of: ECO:0000042 ! gain-of-function mutant phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007093 +name: voucher specimen phenotypic analysis evidence used in manual assertion +def: "A type of voucher specimen phenotypic analysis evidence that is used in a manual assertion." [ECO:RCT] +synonym: "voucher specimen analysis evidence used in manual assertion" EXACT [] +is_a: ECO:0000050 {is_inferred="true"} ! voucher specimen phenotypic analysis evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0000050 ! voucher specimen phenotypic analysis evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007094 +name: positional similarity evidence used in manual assertion +def: "A type of positional similarity evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000060 {is_inferred="true"} ! positional similarity evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +intersection_of: ECO:0000060 ! positional similarity evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007095 +name: quantitative trait analysis evidence used in manual assertion +def: "A type of quantitative trait analysis evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000061 {is_inferred="true"} ! quantitative trait analysis evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0000061 ! quantitative trait analysis evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007096 +name: compositional similarity evidence used in manual assertion +def: "A type of compositional similarity evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000063 {is_inferred="true"} ! compositional similarity evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +intersection_of: ECO:0000063 ! compositional similarity evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007097 +name: developmental similarity evidence used in manual assertion +def: "A type of developmental similarity evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000067 {is_inferred="true"} ! developmental similarity evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +intersection_of: ECO:0000067 ! developmental similarity evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007098 +name: morphological similarity evidence used in manual assertion +def: "A type of morphological similarity evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000071 {is_inferred="true"} ! morphological similarity evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +intersection_of: ECO:0000071 ! morphological similarity evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007099 +name: gene expression similarity evidence used in manual assertion +def: "A type of gene expression similarity evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000075 {is_inferred="true"} ! gene expression similarity evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +intersection_of: ECO:0000075 ! gene expression similarity evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007100 +name: methylation-specific polymerase chain reaction evidence used in manual assertion +def: "A type of methylation-specific polymerase chain reaction evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000077 {is_inferred="true"} ! methylation-specific polymerase chain reaction evidence +is_a: ECO:0001198 {is_inferred="true"} ! in vitro methylation assay evidence used in manual assertion +intersection_of: ECO:0000077 ! methylation-specific polymerase chain reaction evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007101 +name: southern hybridization evidence used in manual assertion +def: "A type of southern hybridization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000078 {is_inferred="true"} ! southern hybridization evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000078 ! southern hybridization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007102 +name: intermethylated site amplification evidence used in manual assertion +def: "A type of intermethylated site amplification evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000086 {is_inferred="true"} ! amplification of intermethylated sites evidence +is_a: ECO:0001198 {is_inferred="true"} ! in vitro methylation assay evidence used in manual assertion +intersection_of: ECO:0000086 ! amplification of intermethylated sites evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007103 +name: epitope-tagged protein immunolocalization evidence used in manual assertion +def: "A type of epitope-tagged protein immunolocalization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000092 {is_inferred="true"} ! epitope-tagged protein immunolocalization evidence +is_a: ECO:0006031 {is_inferred="true"} ! immunolocalization evidence used in manual assertion +intersection_of: ECO:0000092 ! epitope-tagged protein immunolocalization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007104 +name: co-fractionation evidence used in manual assertion +def: "A type of co-fractionation evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000102 {is_inferred="true"} ! co-fractionation evidence +is_a: ECO:0006005 {is_inferred="true"} ! fractionation evidence used in manual assertion +intersection_of: ECO:0000102 ! co-fractionation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007106 +name: green fluorescent protein fusion protein localization evidence used in manual assertion +def: "A type of green fluorescent protein fusion protein localization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000126 {is_inferred="true"} ! green fluorescent protein fusion protein localization evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000126 ! green fluorescent protein fusion protein localization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007107 +name: yellow fluorescent protein fusion protein localization evidence used in manual assertion +def: "A type of yellow fluorescent protein fusion protein localization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000128 {is_inferred="true"} ! yellow fluorescent protein fusion protein localization evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000128 ! yellow fluorescent protein fusion protein localization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007108 +name: beta-glucuronidase fusion protein localization evidence used in manual assertion +def: "A type of beta-glucuronidase fusion protein localization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000130 {is_inferred="true"} ! beta-glucuronidase fusion protein localization evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000130 ! beta-glucuronidase fusion protein localization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007109 +name: beta-galactosidase fusion protein localization evidence used in manual assertion +def: "A type of beta-galactosidase fusion protein localization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000132 {is_inferred="true"} ! beta-galactosidase fusion protein localization evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000132 ! beta-galactosidase fusion protein localization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007110 +name: thin layer chromatography evidence used in manual assertion +def: "A type of thin layer chromatography evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000140 {is_inferred="true"} ! thin layer chromatography evidence +is_a: ECO:0007680 {is_inferred="true"} ! chromatography evidence used in manual assertion +intersection_of: ECO:0000140 ! thin layer chromatography evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007111 +name: in vitro recombinant protein transcription reconstitution assay evidence used in manual assertion +def: "A type of in vitro recombinant protein transcription reconstitution assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000152 {is_inferred="true"} ! in vitro recombinant protein transcription reconstitution assay evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000152 ! in vitro recombinant protein transcription reconstitution assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007112 +name: protein separation followed by direct sequencing evidence used in manual assertion +def: "A type of protein separation followed by direct sequencing evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000158 {is_inferred="true"} ! protein separation followed by direct sequencing evidence +is_a: ECO:0006005 {is_inferred="true"} ! fractionation evidence used in manual assertion +intersection_of: ECO:0000158 ! protein separation followed by direct sequencing evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007113 +name: protein separation followed by fragment identification evidence used in manual assertion +def: "A type of protein separation followed by fragment identification evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000160 {is_inferred="true"} ! protein separation followed by fragment identification evidence +is_a: ECO:0006005 {is_inferred="true"} ! fractionation evidence used in manual assertion +intersection_of: ECO:0000160 ! protein separation followed by fragment identification evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007114 +name: heterologous system uptake evidence used in manual assertion +def: "A type of heterologous system uptake evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000162 {is_inferred="true"} ! heterologous system uptake evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000162 ! heterologous system uptake evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007115 +name: two-electrode voltage clamp recording evidence used in manual assertion +def: "A type of two-electrode voltage clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000166 {is_inferred="true"} ! two-electrode voltage clamp recording evidence +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +intersection_of: ECO:0000166 ! two-electrode voltage clamp recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007116 +name: biochemical trait analysis evidence used in manual assertion +def: "A type of biochemical trait analysis evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000172 {is_inferred="true"} ! biochemical trait analysis evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0000172 ! biochemical trait analysis evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007117 +name: mutant physiological response evidence used in manual assertion +def: "A type of mutant physiological response evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000174 {is_inferred="true"} ! mutant physiological response evidence +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +intersection_of: ECO:0000174 ! mutant physiological response evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007118 +name: mutant visible phenotype evidence used in manual assertion +def: "A type of mutant visible phenotype evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000176 {is_inferred="true"} ! mutant visible phenotype evidence +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +intersection_of: ECO:0000176 ! mutant visible phenotype evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007119 +name: in vivo assay evidence used in manual assertion +def: "A type of in vivo assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000178 {is_inferred="true"} ! in vivo assay evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000178 ! in vivo assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007120 +name: animal model system study evidence used in manual assertion +def: "A type of animal model system study evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000179 {is_inferred="true"} ! animal model system study evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000179 ! animal model system study evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007121 +name: clinical study evidence used in manual assertion +def: "A type of clinical study evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000180 {is_inferred="true"} ! clinical study evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000180 ! clinical study evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007122 +name: in vitro assay evidence used in manual assertion +def: "A type of in vitro assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000181 {is_inferred="true"} ! in vitro assay evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000181 ! in vitro assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007123 +name: enzyme inhibition evidence used in manual assertion +def: "A type of enzyme inhibition evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000184 {is_inferred="true"} ! enzyme inhibition evidence +is_a: ECO:0005801 {is_inferred="true"} ! enzymatic activity assay evidence used in manual assertion +is_a: ECO:0007707 {is_inferred="true"} ! protein inhibition evidence used in manual assertion +intersection_of: ECO:0000184 ! enzyme inhibition evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007125 +name: Illumina sequencing evidence used in manual assertion +def: "A type of Illumina sequencing evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000222 {is_inferred="true"} ! Illumina sequencing evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000222 ! Illumina sequencing evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007126 +name: 454 pyrosequencing evidence used in manual assertion +def: "A type of 454 pyrosequencing evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000223 {is_inferred="true"} ! 454 pyrosequencing evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000223 ! 454 pyrosequencing evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007127 +name: SOLiD sequencing evidence used in manual assertion +def: "A type of SOLiD sequencing evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000224 {is_inferred="true"} ! SOLiD sequencing evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000224 ! SOLiD sequencing evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007128 +name: chain termination sequencing evidence used in manual assertion +def: "A type of chain termination sequencing evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000225 {is_inferred="true"} ! chain termination sequencing evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000225 ! chain termination sequencing evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007129 +name: chromatin immunoprecipitation-qPCR evidence used in manual assertion +def: "A type of chromatin immunoprecipitation-qPCR evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000228 {is_inferred="true"} ! chromatin immunoprecipitation-qPCR evidence +is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion +intersection_of: ECO:0000228 ! chromatin immunoprecipitation-qPCR evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007130 +name: 4C evidence used in manual assertion +def: "A type of 4C evidence that is used in a manual assertion." [ECO:RCT] +synonym: "circularized chromosome conformation capture evidence used in manual assertion" EXACT [] +is_a: ECO:0000234 {is_inferred="true"} ! 4C evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000234 ! 4C evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007131 +name: 5C evidence used in manual assertion +def: "A type of 5C evidence that is used in a manual assertion." [ECO:RCT] +synonym: "carbon-copy chromosome conformation capture evidence used in manual assertion" EXACT [] +is_a: ECO:0000235 {is_inferred="true"} ! 5C evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000235 ! 5C evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007133 +name: 3C-qPCR evidence used in manual assertion +def: "A type of 3C-qPCR evidence that is used in a manual assertion." [ECO:RCT] +synonym: "chromosome conformation capture-qPCR evidence used in manual assertion" EXACT [] +is_a: ECO:0000237 {is_inferred="true"} ! 3C-qPCR evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000237 ! 3C-qPCR evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007134 +name: Hi-C evidence used in manual assertion +def: "A type of Hi-C evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000238 {is_inferred="true"} ! Hi-C evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000238 ! Hi-C evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007135 +name: 3C-seq evidence used in manual assertion +def: "A type of 3C-seq evidence that is used in a manual assertion." [ECO:RCT] +synonym: "chromosome conformation capture sequencing evidence used in manual assertion" EXACT [] +is_a: ECO:0000239 {is_inferred="true"} ! 3C-seq evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000239 ! 3C-seq evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007136 +name: environmental perturbation phenotypic evidence used in manual assertion +def: "A type of environmental perturbation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "environmental perturbation evidence used in manual assertion" EXACT [] +is_a: ECO:0000241 {is_inferred="true"} ! environmental perturbation phenotypic evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0000241 ! environmental perturbation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007137 +name: tissue ablation phenotypic evidence used in manual assertion +def: "A type of tissue ablation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "tissue ablation evidence used in manual assertion" EXACT [] +is_a: ECO:0000242 {is_inferred="true"} ! tissue ablation phenotypic evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0000242 ! tissue ablation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007138 +name: tissue grafting phenotypic evidence used in manual assertion +def: "A type of tissue grafting phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "tissue grafting evidence used in manual assertion" EXACT [] +is_a: ECO:0000243 {is_inferred="true"} ! tissue grafting phenotypic evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0000243 ! tissue grafting phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007141 +name: cytochalasin experiment evidence used in manual assertion +def: "A type of cytochalasin experiment evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000299 {is_inferred="true"} ! cytochalasin experiment evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0000299 ! cytochalasin experiment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007142 +name: green fluorescent protein immunolocalization evidence used in manual assertion +def: "A type of green fluorescent protein immunolocalization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000300 {is_inferred="true"} ! green fluorescent protein immunolocalization evidence +is_a: ECO:0006031 {is_inferred="true"} ! immunolocalization evidence used in manual assertion +intersection_of: ECO:0000300 ! green fluorescent protein immunolocalization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007143 +name: beta-galactosidase protein immunolocalization evidence used in manual assertion +def: "A type of beta-galactosidase protein immunolocalization evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000301 {is_inferred="true"} ! beta-galactosidase protein immunolocalization evidence +is_a: ECO:0006031 {is_inferred="true"} ! immunolocalization evidence used in manual assertion +intersection_of: ECO:0000301 ! beta-galactosidase protein immunolocalization evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007144 +name: cap analysis of gene expression evidence used in manual assertion +def: "A type of cap analysis of gene expression evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion +is_a: ECO:0000309 {is_inferred="true"} ! cap analysis of gene expression evidence +intersection_of: ECO:0000309 ! cap analysis of gene expression evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007145 +name: nano-cap analysis of gene expression evidence used in manual assertion +def: "A type of nano-cap analysis of gene expression evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion +is_a: ECO:0000310 {is_inferred="true"} ! nano-cap analysis of gene expression evidence +intersection_of: ECO:0000310 ! nano-cap analysis of gene expression evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007146 +name: particle size and count assay evidence used in manual assertion +def: "A type of particle size and count assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0000334 {is_inferred="true"} ! particle size and count assay evidence +intersection_of: ECO:0000334 ! particle size and count assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007147 +name: competitive growth assay evidence used in manual assertion +def: "A type of competitive growth assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0000336 {is_inferred="true"} ! competitive growth assay evidence +intersection_of: ECO:0000336 ! competitive growth assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007148 +name: pulsed-field gel electrophoresis evidence used in manual assertion +def: "A type of pulsed-field gel electrophoresis evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0000338 {is_inferred="true"} ! pulsed-field gel electrophoresis evidence +intersection_of: ECO:0000338 ! pulsed-field gel electrophoresis evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007149 +name: two-dimensional agarose gel electrophoresis evidence used in manual assertion +def: "A type of two-dimensional agarose gel electrophoresis evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0000339 {is_inferred="true"} ! two-dimensional agarose gel electrophoresis evidence +intersection_of: ECO:0000339 ! two-dimensional agarose gel electrophoresis evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007150 +name: plasmid maintenance assay evidence used in manual assertion +def: "A type of plasmid maintenance assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0000340 {is_inferred="true"} ! plasmid maintenance assay evidence +intersection_of: ECO:0000340 ! plasmid maintenance assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007151 +name: specific protein inhibition by antibody evidence used in manual assertion +def: "A type of specific protein inhibition by antibody evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000341 {is_inferred="true"} ! specific protein inhibition by antibody evidence +is_a: ECO:0007707 {is_inferred="true"} ! protein inhibition evidence used in manual assertion +intersection_of: ECO:0000341 ! specific protein inhibition by antibody evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007152 +name: single exon transcript confirmation via alignment evidence used in manual assertion +def: "A type of single exon transcript confirmation via alignment evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion +is_a: ECO:0000345 {is_inferred="true"} ! single exon transcript confirmation via alignment evidence +intersection_of: ECO:0000345 ! single exon transcript confirmation via alignment evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "ISA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007153 +name: phylogenetic distribution evidence used in manual assertion +def: "A type of phylogenetic distribution evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +is_a: ECO:0000355 {is_inferred="true"} ! phylogenetic distribution evidence +intersection_of: ECO:0000355 ! phylogenetic distribution evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007154 +name: differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) used in manual assertion +def: "A type of differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion +is_a: ECO:0000358 {is_inferred="true"} ! differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) +intersection_of: ECO:0000358 ! differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007155 +name: differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) used in manual assertion +def: "A type of differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000291 {is_inferred="true"} ! transcript expression evidence used in manual assertion +is_a: ECO:0000359 {is_inferred="true"} ! differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) +intersection_of: ECO:0000359 ! differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007156 +name: biological target-disease association via drug evidence used in manual assertion +def: "A type of biological target-disease association via drug that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0000360 {is_inferred="true"} ! biological target-disease association via drug evidence +intersection_of: ECO:0000360 ! biological target-disease association via drug evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007157 +name: cell staining evidence used in manual assertion +def: "A type of cell staining evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001603 {is_inferred="true"} ! cell staining evidence +intersection_of: ECO:0001603 ! cell staining evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007158 +name: visual sequence inspection evidence used in manual assertion +def: "A type of visual sequence inspection evidence that is used in a manual assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007159 +name: ATP bioluminescence assay evidence used in manual assertion +def: "A type of ATP bioluminescence assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005011 {is_inferred="true"} ! ATP bioluminescence assay evidence +intersection_of: ECO:0005011 ! ATP bioluminescence assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007160 +name: missense mutation pohenotypic evidence used in manual assertion +def: "A type of missense mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "missense mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0001247 {is_inferred="true"} ! point mutation phenotypic evidence used in manual assertion +is_a: ECO:0005506 {is_inferred="true"} ! missense mutation phenotypic evidence +intersection_of: ECO:0005506 ! missense mutation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007161 +name: nonsense mutation phenotypic evidence used in manual assertion +def: "A type of nonsense mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "nonsense mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0001247 {is_inferred="true"} ! point mutation phenotypic evidence used in manual assertion +is_a: ECO:0005507 {is_inferred="true"} ! nonsense mutation phenotypic evidence +intersection_of: ECO:0005507 ! nonsense mutation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007162 +name: silent mutation evidence used in manual assertion +def: "A type of silent mutation evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001247 {is_inferred="true"} ! point mutation phenotypic evidence used in manual assertion +is_a: ECO:0005508 {is_inferred="true"} ! silent mutation evidence +intersection_of: ECO:0005508 ! silent mutation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007163 +name: insertion mutation phenotypic evidence used in manual assertion +def: "A type of insertion mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "insertion mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005509 {is_inferred="true"} ! insertion mutation phenotypic evidence +intersection_of: ECO:0005509 ! insertion mutation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007164 +name: duplication mutation evidence used in manual assertion +def: "A type of duplication mutation evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005511 {is_inferred="true"} ! duplication mutation evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +intersection_of: ECO:0005511 ! duplication mutation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007165 +name: frameshift mutation phenotypic evidence used in manual assertion +def: "A type of frameshift mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "frameshift mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005512 {is_inferred="true"} ! frameshift mutation phenotypic evidence +intersection_of: ECO:0005512 ! frameshift mutation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007166 +name: repeat expansion mutation phenotypic evidence used in manual assertion +def: "A type of repeat expansion mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "repeat expansion mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005513 {is_inferred="true"} ! repeat expansion mutation phenotypic evidence +intersection_of: ECO:0005513 ! repeat expansion mutation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007167 +name: splice site mutation phenotypic evidence used in manual assertion +def: "A type of splice site mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "splice site mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005514 {is_inferred="true"} ! splice site mutation phenotypic evidence +intersection_of: ECO:0005514 ! splice site mutation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007168 +name: translocation mutation phenotypic evidence used in manual assertion +def: "A type of translocation mutation phenotypic evidence that is used in a manual assertion." [ECO:RCT] +synonym: "translocation mutation evidence used in manual assertion" EXACT [] +is_a: ECO:0000315 {is_inferred="true"} ! mutant phenotype evidence used in manual assertion +is_a: ECO:0005515 {is_inferred="true"} ! translocation mutation phenotypic evidence +intersection_of: ECO:0005515 ! translocation mutation phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IMP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007169 +name: in-gel protein kinase assay evidence used in manual assertion +def: "A type of in-gel protein kinase assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005562 {is_inferred="true"} ! in-gel protein kinase assay evidence +is_a: ECO:0005801 {is_inferred="true"} ! enzymatic activity assay evidence used in manual assertion +intersection_of: ECO:0005562 ! in-gel protein kinase assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007170 +name: macroscopic current trace evidence used in manual assertion +def: "A type of macroscopic current trace evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005563 {is_inferred="true"} ! macroscopic current trace evidence +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +intersection_of: ECO:0005563 ! macroscopic current trace evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007171 +name: current density evidence used in manual assertion +def: "A type of current density evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005564 {is_inferred="true"} ! current density evidence +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +intersection_of: ECO:0005564 ! current density evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007172 +name: sustained current evidence used in manual assertion +def: "A type of sustained current evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005566 {is_inferred="true"} ! sustained current evidence +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +intersection_of: ECO:0005566 ! sustained current evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007173 +name: use dependence of inactivation evidence used in manual assertion +def: "A type of use dependence of inactivation evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005570 {is_inferred="true"} ! use dependence of inactivation evidence +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +intersection_of: ECO:0005570 ! use dependence of inactivation evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007174 +name: current clamp recording evidence used in manual assertion +def: "A type of current clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005571 {is_inferred="true"} ! current clamp recording evidence +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +intersection_of: ECO:0005571 ! current clamp recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007175 +name: whole-cell voltage clamp recording evidence used in manual assertion +def: "A type of whole-cell voltage clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005572 {is_inferred="true"} ! whole-cell voltage clamp recording evidence +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +intersection_of: ECO:0005572 ! whole-cell voltage clamp recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007176 +name: cell-attached single-channel recording evidence used in manual assertion +def: "A type of cell-attached single-channel recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005573 {is_inferred="true"} ! cell-attached single-channel recording evidence +is_a: ECO:0006013 {is_inferred="true"} ! patch-clamp recording evidence used in manual assertion +intersection_of: ECO:0005573 ! cell-attached single-channel recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007177 +name: cell-detached inside-out single-channel recording evidence used in manual assertion +def: "A type of cell-detached inside-out single-channel recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005574 {is_inferred="true"} ! cell-detached inside-out single-channel recording evidence +is_a: ECO:0006013 {is_inferred="true"} ! patch-clamp recording evidence used in manual assertion +intersection_of: ECO:0005574 ! cell-detached inside-out single-channel recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007178 +name: reconstituted bilayer single-channel patch recording evidence used in manual assertion +def: "A type of reconstituted bilayer single-channel patch recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005575 {is_inferred="true"} ! reconstituted bilayer single-channel patch recording evidence +is_a: ECO:0006013 {is_inferred="true"} ! patch-clamp recording evidence used in manual assertion +intersection_of: ECO:0005575 ! reconstituted bilayer single-channel patch recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007179 +name: electroencephalography recording evidence used in manual assertion +def: "A type of electroencephalography recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005577 {is_inferred="true"} ! electroencephalography recording evidence +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +intersection_of: ECO:0005577 ! electroencephalography recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007180 +name: cell-detached outside-out single-channel recording evidence used in manual assertion +def: "A type of cell-detached outside-out single-channel recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005582 {is_inferred="true"} ! cell-detached outside-out single-channel recording evidence +is_a: ECO:0006013 {is_inferred="true"} ! patch-clamp recording evidence used in manual assertion +intersection_of: ECO:0005582 ! cell-detached outside-out single-channel recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007181 +name: cut-open oocyte voltage clamp recording evidence used in manual assertion +def: "A type of cut-open oocyte voltage clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005583 {is_inferred="true"} ! cut-open oocyte voltage clamp recording evidence +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +intersection_of: ECO:0005583 ! cut-open oocyte voltage clamp recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007182 +name: macropatch voltage clamp recording evidence used in manual assertion +def: "A type of macropatch voltage clamp recording evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0005584 {is_inferred="true"} ! macropatch voltage clamp recording evidence +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +intersection_of: ECO:0005584 ! macropatch voltage clamp recording evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007184 +name: protein mass spectrometry evidence used in manual assertion +def: "A type of protein mass spectrometry evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001230 {is_inferred="true"} ! mass spectrometry evidence used in manual assertion +is_a: ECO:0005603 {is_inferred="true"} ! protein mass spectrometry evidence +intersection_of: ECO:0005603 ! protein mass spectrometry evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007185 +name: cross-streak test evidence used in manual assertion +def: "A type of cross-streak test evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005604 {is_inferred="true"} ! cross-streak test evidence +intersection_of: ECO:0005604 ! cross-streak test evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007186 +name: tethered cell assay evidence used in manual assertion +def: "A type of tethered cell assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005607 {is_inferred="true"} ! tethered cell assay evidence +intersection_of: ECO:0005607 ! tethered cell assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007187 +name: tumble frequency assay evidence used in manual assertion +def: "A type of tumble frequency assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005608 {is_inferred="true"} ! tumble frequency assay evidence +intersection_of: ECO:0005608 ! tumble frequency assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007188 +name: capillary assay evidence used in manual assertion +def: "A type of capillary assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005609 {is_inferred="true"} ! capillary assay evidence +intersection_of: ECO:0005609 ! capillary assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007189 +name: inference from experimental data evidence used in manual assertion +def: "A type of inference from experimental data evidence that is used in a manual assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007190 +name: inference from phenotype manipulation evidence used in manual assertion +def: "A type of inference from phenotype manipulation evidence that is used in a manual assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007191 +name: inference by association of genotype from phenotype used in manual assertion +def: "A type of inference by association of genotype from phenotype that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0001237 {is_inferred="true"} ! natural variation mutant evidence used in manual assertion +intersection_of: ECO:0005613 ! inference by association of genotype from phenotype +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007192 +name: motility assay evidence used in manual assertion +def: "A type of motility assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005803 {is_inferred="true"} ! motility assay evidence +intersection_of: ECO:0005803 ! motility assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007193 +name: loss-of-function mutant phenotype evidence used in automatic assertion +def: "A type of loss-of-function mutant phenotype evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000016 {is_inferred="true"} ! loss-of-function mutant phenotype evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0000016 ! loss-of-function mutant phenotype evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007194 +name: structural similarity evidence used in automatic assertion +def: "A type of structural similarity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000027 {is_inferred="true"} ! structural similarity evidence +is_a: ECO:0007824 {is_inferred="true"} ! phenotypic similarity evidence used in automatic assertion +intersection_of: ECO:0000027 ! structural similarity evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007196 +name: gain-of-function mutant phenotypic evidence used in automatic assertion +def: "A type of gain-of-function mutant phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "gain-of-function mutant phenotype evidence used in automatic assertion" EXACT [] +is_a: ECO:0000042 {is_inferred="true"} ! gain-of-function mutant phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0000042 ! gain-of-function mutant phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007197 +name: voucher specimen phenotypic analysis evidence used in automatic assertion +def: "A type of voucher specimen phenotypic analysis evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "voucher specimen analysis evidence used in automatic assertion" EXACT [] +is_a: ECO:0000050 {is_inferred="true"} ! voucher specimen phenotypic analysis evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0000050 ! voucher specimen phenotypic analysis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007198 +name: positional similarity evidence used in automatic assertion +def: "A type of positional similarity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000060 {is_inferred="true"} ! positional similarity evidence +is_a: ECO:0007824 {is_inferred="true"} ! phenotypic similarity evidence used in automatic assertion +intersection_of: ECO:0000060 ! positional similarity evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007199 +name: quantitative trait analysis evidence used in automatic assertion +def: "A type of quantitative trait analysis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000061 {is_inferred="true"} ! quantitative trait analysis evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0000061 ! quantitative trait analysis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007200 +name: compositional similarity evidence used in automatic assertion +def: "A type of compositional similarity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000063 {is_inferred="true"} ! compositional similarity evidence +is_a: ECO:0007824 {is_inferred="true"} ! phenotypic similarity evidence used in automatic assertion +intersection_of: ECO:0000063 ! compositional similarity evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007201 +name: developmental similarity evidence used in automatic assertion +def: "A type of developmental similarity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000067 {is_inferred="true"} ! developmental similarity evidence +is_a: ECO:0007824 {is_inferred="true"} ! phenotypic similarity evidence used in automatic assertion +intersection_of: ECO:0000067 ! developmental similarity evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007202 +name: morphological similarity evidence used in automatic assertion +def: "A type of morphological similarity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000071 {is_inferred="true"} ! morphological similarity evidence +is_a: ECO:0007824 {is_inferred="true"} ! phenotypic similarity evidence used in automatic assertion +intersection_of: ECO:0000071 ! morphological similarity evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007203 +name: gene expression similarity evidence used in automatic assertion +def: "A type of gene expression similarity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000075 {is_inferred="true"} ! gene expression similarity evidence +is_a: ECO:0007824 {is_inferred="true"} ! phenotypic similarity evidence used in automatic assertion +intersection_of: ECO:0000075 ! gene expression similarity evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007204 +name: methylation-specific polymerase chain reaction evidence used in automatic assertion +def: "A type of methylation-specific polymerase chain reaction evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000077 {is_inferred="true"} ! methylation-specific polymerase chain reaction evidence +is_a: ECO:0007401 {is_inferred="true"} ! in vitro methylation assay evidence used in automatic assertion +intersection_of: ECO:0000077 ! methylation-specific polymerase chain reaction evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007205 +name: southern hybridization evidence used in automatic assertion +def: "A type of southern hybridization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000078 {is_inferred="true"} ! southern hybridization evidence +is_a: ECO:0007817 {is_inferred="true"} ! DNA detection assay evidence used in automatic assertion +intersection_of: ECO:0000078 ! southern hybridization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007206 +name: intermethylated site amplification evidence used in automatic assertion +def: "A type of intermethylated site amplification evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000086 {is_inferred="true"} ! amplification of intermethylated sites evidence +is_a: ECO:0007401 {is_inferred="true"} ! in vitro methylation assay evidence used in automatic assertion +intersection_of: ECO:0000086 ! amplification of intermethylated sites evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007207 +name: epitope-tagged protein immunolocalization evidence used in automatic assertion +def: "A type of epitope-tagged protein immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000092 {is_inferred="true"} ! epitope-tagged protein immunolocalization evidence +is_a: ECO:0007486 {is_inferred="true"} ! immunolocalization evidence used in automatic assertion +intersection_of: ECO:0000092 ! epitope-tagged protein immunolocalization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007208 +name: co-fractionation evidence used in automatic assertion +def: "A type of co-fractionation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000102 {is_inferred="true"} ! co-fractionation evidence +is_a: ECO:0007534 {is_inferred="true"} ! fractionation evidence used in automatic assertion +intersection_of: ECO:0000102 ! co-fractionation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007210 +name: green fluorescent protein fusion protein localization evidence used in automatic assertion +def: "A type of green fluorescent protein fusion protein localization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000126 {is_inferred="true"} ! green fluorescent protein fusion protein localization evidence +is_a: ECO:0007808 {is_inferred="true"} ! fusion protein localization evidence used in automatic assertion +intersection_of: ECO:0000126 ! green fluorescent protein fusion protein localization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007211 +name: yellow fluorescent protein fusion protein localization evidence used in automatic assertion +def: "A type of yellow fluorescent protein fusion protein localization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000128 {is_inferred="true"} ! yellow fluorescent protein fusion protein localization evidence +is_a: ECO:0007808 {is_inferred="true"} ! fusion protein localization evidence used in automatic assertion +intersection_of: ECO:0000128 ! yellow fluorescent protein fusion protein localization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007212 +name: beta-glucuronidase fusion protein localization evidence used in automatic assertion +def: "A type of beta-glucuronidase fusion protein localization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000130 {is_inferred="true"} ! beta-glucuronidase fusion protein localization evidence +is_a: ECO:0007808 {is_inferred="true"} ! fusion protein localization evidence used in automatic assertion +intersection_of: ECO:0000130 ! beta-glucuronidase fusion protein localization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007213 +name: beta-galactosidase fusion protein localization evidence used in automatic assertion +def: "A type of beta-galactosidase fusion protein localization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000132 {is_inferred="true"} ! beta-galactosidase fusion protein localization evidence +is_a: ECO:0007808 {is_inferred="true"} ! fusion protein localization evidence used in automatic assertion +intersection_of: ECO:0000132 ! beta-galactosidase fusion protein localization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007214 +name: thin layer chromatography evidence used in automatic assertion +def: "A type of thin layer chromatography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000140 {is_inferred="true"} ! thin layer chromatography evidence +is_a: ECO:0007681 {is_inferred="true"} ! chromatography evidence used in automatic assertion +intersection_of: ECO:0000140 ! thin layer chromatography evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007215 +name: in vitro recombinant protein transcription reconstitution assay evidence used in automatic assertion +def: "A type of in vitro recombinant protein transcription reconstitution assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000152 {is_inferred="true"} ! in vitro recombinant protein transcription reconstitution assay evidence +is_a: ECO:0007804 {is_inferred="true"} ! in vitro transcription reconstitution assay evidence used in automatic assertion +intersection_of: ECO:0000152 ! in vitro recombinant protein transcription reconstitution assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007216 +name: protein separation followed by direct sequencing evidence used in automatic assertion +def: "A type of protein separation followed by direct sequencing evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000158 {is_inferred="true"} ! protein separation followed by direct sequencing evidence +is_a: ECO:0007787 {is_inferred="true"} ! protein separation evidence used in automatic assertion +intersection_of: ECO:0000158 ! protein separation followed by direct sequencing evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007217 +name: protein separation followed by fragment identification evidence used in automatic assertion +def: "A type of protein separation followed by fragment identification evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000160 {is_inferred="true"} ! protein separation followed by fragment identification evidence +is_a: ECO:0007787 {is_inferred="true"} ! protein separation evidence used in automatic assertion +intersection_of: ECO:0000160 ! protein separation followed by fragment identification evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007218 +name: heterologous system uptake evidence used in automatic assertion +def: "A type of heterologous system uptake evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000162 {is_inferred="true"} ! heterologous system uptake evidence +is_a: ECO:0007789 {is_inferred="true"} ! transport assay evidence used in automatic assertion +intersection_of: ECO:0000162 ! heterologous system uptake evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007219 +name: two-electrode voltage clamp recording evidence used in automatic assertion +def: "A type of two-electrode voltage clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000166 {is_inferred="true"} ! two-electrode voltage clamp recording evidence +is_a: ECO:0007766 {is_inferred="true"} ! voltage clamp recording evidence used in automatic assertion +intersection_of: ECO:0000166 ! two-electrode voltage clamp recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007220 +name: biochemical trait analysis evidence used in automatic assertion +def: "A type of biochemical trait analysis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000172 {is_inferred="true"} ! biochemical trait analysis evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0000172 ! biochemical trait analysis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007221 +name: mutant physiological response evidence used in automatic assertion +def: "A type of mutant physiological response evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000174 {is_inferred="true"} ! mutant physiological response evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0000174 ! mutant physiological response evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007222 +name: mutant visible phenotype evidence used in automatic assertion +def: "A type of mutant visible phenotype evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000176 {is_inferred="true"} ! mutant visible phenotype evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0000176 ! mutant visible phenotype evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007223 +name: in vivo assay evidence used in automatic assertion +def: "A type of in vivo assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000178 {is_inferred="true"} ! in vivo assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000178 ! in vivo assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007224 +name: animal model system study evidence used in automatic assertion +def: "A type of animal model system study evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000179 {is_inferred="true"} ! animal model system study evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0000179 ! animal model system study evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007225 +name: clinical study evidence used in automatic assertion +def: "A type of clinical study evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000180 {is_inferred="true"} ! clinical study evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0000180 ! clinical study evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007226 +name: in vitro assay evidence used in automatic assertion +def: "A type of in vitro assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000181 {is_inferred="true"} ! in vitro assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000181 ! in vitro assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007227 +name: enzyme inhibition evidence used in automatic assertion +def: "A type of enzyme inhibition evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000184 {is_inferred="true"} ! enzyme inhibition evidence +is_a: ECO:0007468 {is_inferred="true"} ! enzymatic activity assay evidence used in automatic assertion +is_a: ECO:0007785 {is_inferred="true"} ! protein inhibition evidence used in automatic assertion +intersection_of: ECO:0000184 ! enzyme inhibition evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007229 +name: Illumina sequencing evidence used in automatic assertion +def: "A type of Illumina sequencing evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000222 {is_inferred="true"} ! Illumina sequencing evidence +is_a: ECO:0007814 {is_inferred="true"} ! high throughput nucleotide sequencing assay evidence used in automatic assertion +intersection_of: ECO:0000222 ! Illumina sequencing evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007230 +name: 454 pyrosequencing evidence used in automatic assertion +def: "A type of 454 pyrosequencing evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000223 {is_inferred="true"} ! 454 pyrosequencing evidence +is_a: ECO:0007814 {is_inferred="true"} ! high throughput nucleotide sequencing assay evidence used in automatic assertion +intersection_of: ECO:0000223 ! 454 pyrosequencing evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007231 +name: SOLiD sequencing evidence used in automatic assertion +def: "A type of SOLiD sequencing evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000224 {is_inferred="true"} ! SOLiD sequencing evidence +is_a: ECO:0007814 {is_inferred="true"} ! high throughput nucleotide sequencing assay evidence used in automatic assertion +intersection_of: ECO:0000224 ! SOLiD sequencing evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007232 +name: chain termination sequencing evidence used in automatic assertion +def: "A type of chain termination sequencing evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000225 {is_inferred="true"} ! chain termination sequencing evidence +is_a: ECO:0007813 {is_inferred="true"} ! nucleotide sequencing assay evidence used in automatic assertion +intersection_of: ECO:0000225 ! chain termination sequencing evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007233 +name: chromatin immunoprecipitation-qPCR evidence used in automatic assertion +def: "A type of chromatin immunoprecipitation-qPCR evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000228 {is_inferred="true"} ! chromatin immunoprecipitation-qPCR evidence +is_a: ECO:0007786 {is_inferred="true"} ! chromatin immunoprecipitation evidence used in automatic assertion +intersection_of: ECO:0000228 ! chromatin immunoprecipitation-qPCR evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007234 +name: 4C evidence used in automatic assertion +def: "A type of 4C evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "circularized chromosome conformation capture evidence used in automatic assertion" EXACT [] +is_a: ECO:0000234 {is_inferred="true"} ! 4C evidence +is_a: ECO:0007822 {is_inferred="true"} ! chromosome conformation-based evidence used in automatic assertion +intersection_of: ECO:0000234 ! 4C evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007235 +name: 5C evidence used in automatic assertion +def: "A type of 5C evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "carbon-copy chromosome conformation capture evidence used in automatic assertion" EXACT [] +is_a: ECO:0000235 {is_inferred="true"} ! 5C evidence +is_a: ECO:0007822 {is_inferred="true"} ! chromosome conformation-based evidence used in automatic assertion +intersection_of: ECO:0000235 ! 5C evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007237 +name: 3C-qPCR evidence used in automatic assertion +def: "A type of 3C-qPCR evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "chromosome conformation capture-qPCR evidence used in automatic assertion" EXACT [] +is_a: ECO:0000237 {is_inferred="true"} ! 3C-qPCR evidence +is_a: ECO:0007823 {is_inferred="true"} ! 3C evidence used in automatic assertion +intersection_of: ECO:0000237 ! 3C-qPCR evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007238 +name: Hi-C evidence used in automatic assertion +def: "A type of Hi-C evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000238 {is_inferred="true"} ! Hi-C evidence +is_a: ECO:0007822 {is_inferred="true"} ! chromosome conformation-based evidence used in automatic assertion +intersection_of: ECO:0000238 ! Hi-C evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007239 +name: 3C-seq evidence used in automatic assertion +def: "A type of 3C-seq evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "chromosome conformation capture sequencing evidence used in automatic assertion" EXACT [] +is_a: ECO:0000239 {is_inferred="true"} ! 3C-seq evidence +is_a: ECO:0007822 {is_inferred="true"} ! chromosome conformation-based evidence used in automatic assertion +intersection_of: ECO:0000239 ! 3C-seq evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007240 +name: environmental perturbation phenotypic evidence used in automatic assertion +def: "A type of environmental perturbation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "environmental perturbation evidence used in automatic assertion" EXACT [] +is_a: ECO:0000241 {is_inferred="true"} ! environmental perturbation phenotypic evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0000241 ! environmental perturbation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007241 +name: tissue ablation phenotypic evidence used in automatic assertion +def: "A type of tissue ablation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "tissue ablation evidence used in automatic assertion" EXACT [] +is_a: ECO:0000242 {is_inferred="true"} ! tissue ablation phenotypic evidence +is_a: ECO:0007810 {is_inferred="true"} ! anatomical perturbation phenotypic evidence used in automatic assertion +intersection_of: ECO:0000242 ! tissue ablation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007242 +name: tissue grafting phenotypic evidence used in automatic assertion +def: "A type of tissue grafting phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "tissue grafting evidence used in automatic assertion" EXACT [] +is_a: ECO:0000243 {is_inferred="true"} ! tissue grafting phenotypic evidence +is_a: ECO:0007810 {is_inferred="true"} ! anatomical perturbation phenotypic evidence used in automatic assertion +intersection_of: ECO:0000243 ! tissue grafting phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007245 +name: cytochalasin experiment evidence used in automatic assertion +def: "A type of cytochalasin experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000299 {is_inferred="true"} ! cytochalasin experiment evidence +is_a: ECO:0007811 {is_inferred="true"} ! cleavage arrested development evidence used in automatic assertion +intersection_of: ECO:0000299 ! cytochalasin experiment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007246 +name: green fluorescent protein immunolocalization evidence used in automatic assertion +def: "A type of green fluorescent protein immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000300 {is_inferred="true"} ! green fluorescent protein immunolocalization evidence +is_a: ECO:0007486 {is_inferred="true"} ! immunolocalization evidence used in automatic assertion +intersection_of: ECO:0000300 ! green fluorescent protein immunolocalization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007247 +name: beta-galactosidase protein immunolocalization evidence used in automatic assertion +def: "A type of beta-galactosidase protein immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000301 {is_inferred="true"} ! beta-galactosidase protein immunolocalization evidence +is_a: ECO:0007486 {is_inferred="true"} ! immunolocalization evidence used in automatic assertion +intersection_of: ECO:0000301 ! beta-galactosidase protein immunolocalization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007248 +name: cap analysis of gene expression evidence used in automatic assertion +def: "A type of cap analysis of gene expression evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000309 {is_inferred="true"} ! cap analysis of gene expression evidence +is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion +intersection_of: ECO:0000309 ! cap analysis of gene expression evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007249 +name: nano-cap analysis of gene expression evidence used in automatic assertion +def: "A type of nano-cap analysis of gene expression evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000310 {is_inferred="true"} ! nano-cap analysis of gene expression evidence +is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion +intersection_of: ECO:0000310 ! nano-cap analysis of gene expression evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007250 +name: particle size and count assay evidence used in automatic assertion +def: "A type of particle size and count assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000334 {is_inferred="true"} ! particle size and count assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000334 ! particle size and count assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007251 +name: competitive growth assay evidence used in automatic assertion +def: "A type of competitive growth assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000336 {is_inferred="true"} ! competitive growth assay evidence +is_a: ECO:0007775 {is_inferred="true"} ! cell proliferation assay evidence used in automatic assertion +intersection_of: ECO:0000336 ! competitive growth assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007252 +name: pulsed-field gel electrophoresis evidence used in automatic assertion +def: "A type of pulsed-field gel electrophoresis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000338 {is_inferred="true"} ! pulsed-field gel electrophoresis evidence +is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion +intersection_of: ECO:0000338 ! pulsed-field gel electrophoresis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007253 +name: two-dimensional agarose gel electrophoresis evidence used in automatic assertion +def: "A type of two-dimensional agarose gel electrophoresis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000339 {is_inferred="true"} ! two-dimensional agarose gel electrophoresis evidence +is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion +intersection_of: ECO:0000339 ! two-dimensional agarose gel electrophoresis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007254 +name: plasmid maintenance assay evidence used in automatic assertion +def: "A type of plasmid maintenance assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000340 {is_inferred="true"} ! plasmid maintenance assay evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0000340 ! plasmid maintenance assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007255 +name: specific protein inhibition by antibody evidence used in automatic assertion +def: "A type of specific protein inhibition by antibody evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000341 {is_inferred="true"} ! specific protein inhibition by antibody evidence +is_a: ECO:0007785 {is_inferred="true"} ! protein inhibition evidence used in automatic assertion +intersection_of: ECO:0000341 ! specific protein inhibition by antibody evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007256 +name: single exon transcript confirmation via alignment evidence used in automatic assertion +def: "A type of single exon transcript confirmation via alignment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000248 {is_inferred="true"} ! sequence alignment evidence used in automatic assertion +is_a: ECO:0000345 {is_inferred="true"} ! single exon transcript confirmation via alignment evidence +intersection_of: ECO:0000345 ! single exon transcript confirmation via alignment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007257 +name: phylogenetic distribution evidence used in automatic assertion +def: "A type of phylogenetic distribution evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000355 {is_inferred="true"} ! phylogenetic distribution evidence +is_a: ECO:0007826 {is_inferred="true"} ! phylogenetic evidence used in automatic assertion +intersection_of: ECO:0000355 ! phylogenetic distribution evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007258 +name: differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) used in automatic assertion +def: "A type of differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000358 {is_inferred="true"} ! differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) +is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion +intersection_of: ECO:0000358 ! differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007259 +name: differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) used in automatic assertion +def: "A type of differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000359 {is_inferred="true"} ! differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) +is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion +intersection_of: ECO:0000359 ! differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007260 +name: biological target-disease association via drug evidence used in automatic assertion +def: "A type of biological target-disease association via drug that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000360 {is_inferred="true"} ! biological target-disease association via drug evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0000360 ! biological target-disease association via drug evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007261 +name: cell staining evidence used in automatic assertion +def: "A type of cell staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001603 {is_inferred="true"} ! cell staining evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0001603 ! cell staining evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007262 +name: visual sequence inspection evidence used in automatic assertion +def: "A type of visual sequence inspection evidence that is used in an automatic assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007263 +name: ATP bioluminescence assay evidence used in automatic assertion +def: "A type of ATP bioluminescence assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005011 {is_inferred="true"} ! ATP bioluminescence assay evidence +is_a: ECO:0007774 {is_inferred="true"} ! cell viability assay evidence used in automatic assertion +intersection_of: ECO:0005011 ! ATP bioluminescence assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007264 +name: missense mutation phenotypic evidence used in automatic assertion +def: "A type of missense mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "missense mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005506 {is_inferred="true"} ! missense mutation phenotypic evidence +is_a: ECO:0007448 {is_inferred="true"} ! point mutation phenotypic evidence used in automatic assertion +intersection_of: ECO:0005506 ! missense mutation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007265 +name: nonsense mutation phenotypic evidence used in automatic assertion +def: "A type of nonsense mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "nonsense mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005507 {is_inferred="true"} ! nonsense mutation phenotypic evidence +is_a: ECO:0007448 {is_inferred="true"} ! point mutation phenotypic evidence used in automatic assertion +intersection_of: ECO:0005507 ! nonsense mutation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007266 +name: silent mutation evidence used in automatic assertion +def: "A type of silent mutation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005508 {is_inferred="true"} ! silent mutation evidence +is_a: ECO:0007448 {is_inferred="true"} ! point mutation phenotypic evidence used in automatic assertion +intersection_of: ECO:0005508 ! silent mutation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007267 +name: insertion mutation phenotypic evidence used in automatic assertion +def: "A type of insertion mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "insertion mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005509 {is_inferred="true"} ! insertion mutation phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0005509 ! insertion mutation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007268 +name: duplication mutation evidence used in automatic assertion +def: "A type of duplication mutation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005511 {is_inferred="true"} ! duplication mutation evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0005511 ! duplication mutation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007269 +name: frameshift mutation phenotypic evidence used in automatic assertion +def: "A type of frameshift mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "frameshift mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005512 {is_inferred="true"} ! frameshift mutation phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0005512 ! frameshift mutation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007270 +name: repeat expansion mutation phenotypic evidence used in automatic assertion +def: "A type of repeat expansion mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "repeat expansion mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005513 {is_inferred="true"} ! repeat expansion mutation phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0005513 ! repeat expansion mutation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007271 +name: splice site mutation phenotypic evidence used in automatic assertion +def: "A type of splice site mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "splice site mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005514 {is_inferred="true"} ! splice site mutation phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0005514 ! splice site mutation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007272 +name: translocation mutation phenotypic evidence used in automatic assertion +def: "A type of translocation mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "translocation mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0005515 {is_inferred="true"} ! translocation mutation phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0005515 ! translocation mutation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007273 +name: in-gel protein kinase assay evidence used in automatic assertion +def: "A type of in-gel protein kinase assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005562 {is_inferred="true"} ! in-gel protein kinase assay evidence +is_a: ECO:0007767 {is_inferred="true"} ! protein kinase assay evidence used in automatic assertion +intersection_of: ECO:0005562 ! in-gel protein kinase assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007274 +name: macroscopic current trace evidence used in automatic assertion +def: "A type of macroscopic current trace evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005563 {is_inferred="true"} ! macroscopic current trace evidence +is_a: ECO:0007766 {is_inferred="true"} ! voltage clamp recording evidence used in automatic assertion +intersection_of: ECO:0005563 ! macroscopic current trace evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007275 +name: current density evidence used in automatic assertion +def: "A type of current density evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005564 {is_inferred="true"} ! current density evidence +is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion +intersection_of: ECO:0005564 ! current density evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007276 +name: sustained current evidence used in automatic assertion +def: "A type of sustained current evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005566 {is_inferred="true"} ! sustained current evidence +is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion +intersection_of: ECO:0005566 ! sustained current evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007277 +name: use dependence of inactivation evidence used in automatic assertion +def: "A type of use dependence of inactivation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005570 {is_inferred="true"} ! use dependence of inactivation evidence +is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion +intersection_of: ECO:0005570 ! use dependence of inactivation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007278 +name: current clamp recording evidence used in automatic assertion +def: "A type of current clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005571 {is_inferred="true"} ! current clamp recording evidence +is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion +intersection_of: ECO:0005571 ! current clamp recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007279 +name: whole-cell voltage clamp recording evidence used in automatic assertion +def: "A type of whole-cell voltage clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005572 {is_inferred="true"} ! whole-cell voltage clamp recording evidence +is_a: ECO:0007766 {is_inferred="true"} ! voltage clamp recording evidence used in automatic assertion +intersection_of: ECO:0005572 ! whole-cell voltage clamp recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007280 +name: cell-attached single-channel recording evidence used in automatic assertion +def: "A type of cell-attached single-channel recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005573 {is_inferred="true"} ! cell-attached single-channel recording evidence +is_a: ECO:0007537 {is_inferred="true"} ! patch-clamp recording evidence used in automatic assertion +intersection_of: ECO:0005573 ! cell-attached single-channel recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007281 +name: cell-detached inside-out single-channel recording evidence used in automatic assertion +def: "A type of cell-detached inside-out single-channel recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005574 {is_inferred="true"} ! cell-detached inside-out single-channel recording evidence +is_a: ECO:0007537 {is_inferred="true"} ! patch-clamp recording evidence used in automatic assertion +intersection_of: ECO:0005574 ! cell-detached inside-out single-channel recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007282 +name: reconstituted bilayer single-channel patch recording evidence used in automatic assertion +def: "A type of reconstituted bilayer single-channel patch recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005575 {is_inferred="true"} ! reconstituted bilayer single-channel patch recording evidence +is_a: ECO:0007537 {is_inferred="true"} ! patch-clamp recording evidence used in automatic assertion +intersection_of: ECO:0005575 ! reconstituted bilayer single-channel patch recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007283 +name: electroencephalography recording evidence used in automatic assertion +def: "A type of electroencephalography recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005577 {is_inferred="true"} ! electroencephalography recording evidence +is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion +intersection_of: ECO:0005577 ! electroencephalography recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007284 +name: cell-detached outside-out single-channel recording evidence used in automatic assertion +def: "A type of cell-detached outside-out single-channel recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005582 {is_inferred="true"} ! cell-detached outside-out single-channel recording evidence +is_a: ECO:0007537 {is_inferred="true"} ! patch-clamp recording evidence used in automatic assertion +intersection_of: ECO:0005582 ! cell-detached outside-out single-channel recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007285 +name: cut-open oocyte voltage clamp recording evidence used in automatic assertion +def: "A type of cut-open oocyte voltage clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005583 {is_inferred="true"} ! cut-open oocyte voltage clamp recording evidence +is_a: ECO:0007766 {is_inferred="true"} ! voltage clamp recording evidence used in automatic assertion +intersection_of: ECO:0005583 ! cut-open oocyte voltage clamp recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007286 +name: macropatch voltage clamp recording evidence used in automatic assertion +def: "A type of macropatch voltage clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005584 {is_inferred="true"} ! macropatch voltage clamp recording evidence +is_a: ECO:0007766 {is_inferred="true"} ! voltage clamp recording evidence used in automatic assertion +intersection_of: ECO:0005584 ! macropatch voltage clamp recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007288 +name: protein mass spectrometry evidence used in automatic assertion +def: "A type of protein mass spectrometry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005603 {is_inferred="true"} ! protein mass spectrometry evidence +is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion +intersection_of: ECO:0005603 ! protein mass spectrometry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007289 +name: cross-streak test evidence used in automatic assertion +def: "A type of cross-streak test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005604 {is_inferred="true"} ! cross-streak test evidence +is_a: ECO:0007775 {is_inferred="true"} ! cell proliferation assay evidence used in automatic assertion +intersection_of: ECO:0005604 ! cross-streak test evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007290 +name: tethered cell assay evidence used in automatic assertion +def: "A type of tethered cell assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005607 {is_inferred="true"} ! tethered cell assay evidence +is_a: ECO:0007780 {is_inferred="true"} ! chemotaxis assay evidence used in automatic assertion +intersection_of: ECO:0005607 ! tethered cell assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007291 +name: tumble frequency assay evidence used in automatic assertion +def: "A type of tumble frequency assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005608 {is_inferred="true"} ! tumble frequency assay evidence +is_a: ECO:0007780 {is_inferred="true"} ! chemotaxis assay evidence used in automatic assertion +intersection_of: ECO:0005608 ! tumble frequency assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007292 +name: capillary assay evidence used in automatic assertion +def: "A type of capillary assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005609 {is_inferred="true"} ! capillary assay evidence +is_a: ECO:0007780 {is_inferred="true"} ! chemotaxis assay evidence used in automatic assertion +intersection_of: ECO:0005609 ! capillary assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007293 +name: inference from experimental data evidence used in automatic assertion +def: "A type of inference from experimental data evidence that is used in an automatic assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007294 +name: inference from phenotype manipulation evidence used in automatic assertion +def: "A type of inference from phenotype manipulation evidence that is used in an automatic assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007295 +name: inference by association of genotype from phenotype used in automatic assertion +def: "A type of inference by association of genotype from phenotype that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005613 {is_inferred="true"} ! inference by association of genotype from phenotype +is_a: ECO:0007191 {is_inferred="true"} ! inference by association of genotype from phenotype used in manual assertion +is_a: ECO:0007440 {is_inferred="true"} ! natural variation mutant evidence used in automatic assertion +intersection_of: ECO:0005613 ! inference by association of genotype from phenotype +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007296 +name: motility assay evidence used in automatic assertion +def: "A type of motility assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005803 {is_inferred="true"} ! motility assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0005803 ! motility assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007297 +name: experimental evidence used in automatic assertion +def: "A type of experimental evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000006 {is_inferred="true"} ! experimental evidence +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +intersection_of: ECO:0000006 ! experimental evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007298 +name: expression pattern evidence used in automatic assertion +def: "A type of expression pattern evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000008 {is_inferred="true"} ! expression pattern evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0000008 ! expression pattern evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007299 +name: Affymetrix GeneChip evidence used in automatic assertion +def: "A type of Affymetrix GeneChip evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000101 {is_inferred="true"} ! Affymetrix GeneChip evidence +is_a: ECO:0007300 {is_inferred="true"} ! cRNA to DNA expression microarray evidence used in automatic assertion +intersection_of: ECO:0000101 ! Affymetrix GeneChip evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007300 +name: cRNA to DNA expression microarray evidence used in automatic assertion +def: "A type of cRNA to DNA expression microarray evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000062 {is_inferred="true"} ! cRNA to DNA expression microarray evidence +is_a: ECO:0007301 {is_inferred="true"} ! expression microarray evidence used in automatic assertion +intersection_of: ECO:0000062 ! cRNA to DNA expression microarray evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007301 +name: expression microarray evidence used in automatic assertion +def: "A type of expression microarray evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000058 {is_inferred="true"} ! expression microarray evidence +is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion +intersection_of: ECO:0000058 ! expression microarray evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007302 +name: differential methylation hybridization evidence used in automatic assertion +def: "A type of differential methylation hybridization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000069 {is_inferred="true"} ! differential methylation hybridization evidence +is_a: ECO:0007401 {is_inferred="true"} ! in vitro methylation assay evidence used in automatic assertion +intersection_of: ECO:0000069 ! differential methylation hybridization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007303 +name: transcript expression evidence used in automatic assertion +def: "A type of transcript expression evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "transcriptomics evidence used in automatic assertion" EXACT [] +is_a: ECO:0000009 {is_inferred="true"} ! transcript expression evidence +is_a: ECO:0007298 {is_inferred="true"} ! expression pattern evidence used in automatic assertion +intersection_of: ECO:0000009 ! transcript expression evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007304 +name: Nimblegen array evidence used in automatic assertion +def: "A type of Nimblegen array evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000105 {is_inferred="true"} ! Nimblegen array evidence +is_a: ECO:0005630 {is_inferred="true"} ! cDNA to DNA expression microarray evidence used in automatic assertion +intersection_of: ECO:0000105 ! Nimblegen array evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007305 +name: array-based sequence capture evidence used in automatic assertion +def: "A type of array-based sequence capture evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000093 {is_inferred="true"} ! array-based sequence capture evidence +is_a: ECO:0007817 {is_inferred="true"} ! DNA detection assay evidence used in automatic assertion +intersection_of: ECO:0000093 ! array-based sequence capture evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007306 +name: qualitative western immunoblotting evidence used in automatic assertion +alt_id: ECO:0007287 +def: "A type of qualitative western immunoblotting evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "protein expression level evidence based on western blot used in automatic assertion" EXACT [] +is_a: ECO:0000112 {is_inferred="true"} ! qualitative western immunoblotting evidence +is_a: ECO:0007309 {is_inferred="true"} ! protein expression evidence used in automatic assertion +is_a: ECO:0007770 {is_inferred="true"} ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence used in automatic assertion +is_a: ECO:0007796 {is_inferred="true"} ! immunodetection assay evidence used in automatic assertion +intersection_of: ECO:0000112 ! qualitative western immunoblotting evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007307 +name: direct assay evidence used in automatic assertion +def: "A type of direct assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000002 {is_inferred="true"} ! direct assay evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0000002 ! direct assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007309 +name: protein expression evidence used in automatic assertion +alt_id: ECO:0007308 +def: "A type of protein expression evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "protein expression level evidence used in automatic assertion" EXACT [] +is_a: ECO:0000010 {is_inferred="true"} ! protein expression evidence +is_a: ECO:0007298 {is_inferred="true"} ! expression pattern evidence used in automatic assertion +intersection_of: ECO:0000010 ! protein expression evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007310 +name: expression library screen evidence used in automatic assertion +def: "A type of expression library screen evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000114 {is_inferred="true"} ! expression library screen evidence +is_a: ECO:0007309 {is_inferred="true"} ! protein expression evidence used in automatic assertion +intersection_of: ECO:0000114 ! expression library screen evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007311 +name: heterologous protein expression evidence used in automatic assertion +def: "A type of heterologous protein expression evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000154 {is_inferred="true"} ! heterologous protein expression evidence +is_a: ECO:0007309 {is_inferred="true"} ! protein expression evidence used in automatic assertion +intersection_of: ECO:0000154 ! heterologous protein expression evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007312 +name: spatial pattern of protein expression evidence used in automatic assertion +def: "A type of spatial pattern of protein expression evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000045 {is_inferred="true"} ! spatial pattern of protein expression evidence +is_a: ECO:0007309 {is_inferred="true"} ! protein expression evidence used in automatic assertion +intersection_of: ECO:0000045 ! spatial pattern of protein expression evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007313 +name: DNA to cDNA expression microarray evidence used in automatic assertion +def: "A type of DNA to cDNA expression microarray evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000104 {is_inferred="true"} ! DNA to cDNA expression microarray evidence +is_a: ECO:0007301 {is_inferred="true"} ! expression microarray evidence used in automatic assertion +intersection_of: ECO:0000104 ! DNA to cDNA expression microarray evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007314 +name: differential hybridization evidence used in automatic assertion +def: "A type of differential hybridization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000116 {is_inferred="true"} ! differential hybridization evidence +is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion +intersection_of: ECO:0000116 ! differential hybridization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007315 +name: RNA protection assay evidence used in automatic assertion +def: "A type of RNA protection assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000110 {is_inferred="true"} ! RNA protection assay evidence +is_a: ECO:0007316 {is_inferred="true"} ! nuclease protection assay evidence used in automatic assertion +intersection_of: ECO:0000110 ! RNA protection assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007316 +name: nuclease protection assay evidence used in automatic assertion +def: "A type of nuclease protection assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001106 {is_inferred="true"} ! nuclease protection assay evidence +is_a: ECO:0007818 {is_inferred="true"} ! RNA detection assay evidence used in automatic assertion +intersection_of: ECO:0001106 ! nuclease protection assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007317 +name: spatial pattern of transcript expression evidence used in automatic assertion +def: "A type of spatial pattern of transcript expression evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000047 {is_inferred="true"} ! spatial pattern of transcript expression evidence +is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion +intersection_of: ECO:0000047 ! spatial pattern of transcript expression evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007318 +name: subtractive hybridization evidence used in automatic assertion +def: "A type of subtractive hybridization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000118 {is_inferred="true"} ! subtractive hybridization evidence +is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion +intersection_of: ECO:0000118 ! subtractive hybridization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007319 +name: author statement used in automatic assertion +def: "A type of author statement that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000204 {is_inferred="true"} ! author statement +is_a: ECO:0006156 {is_inferred="true"} ! documented statement evidence used in automatic assertion +intersection_of: ECO:0000204 ! author statement +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007320 +name: author statement without traceable support used in automatic assertion +def: "A type of author statement without traceable support that is used in an automatic assertion." [ECO:RCT] +synonym: "non-traceable author statement used in automatic assertion" RELATED [] +is_a: ECO:0000034 {is_inferred="true"} ! author statement without traceable support +is_a: ECO:0007319 {is_inferred="true"} ! author statement used in automatic assertion +intersection_of: ECO:0000034 ! author statement without traceable support +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007321 +name: author statement supported by traceable reference used in automatic assertion +def: "A type of author statement supported by traceable reference that is used in an automatic assertion." [ECO:RCT] +synonym: "traceable author statement used in automatic assertion" RELATED [] +is_a: ECO:0000033 {is_inferred="true"} ! author statement supported by traceable reference +is_a: ECO:0007319 {is_inferred="true"} ! author statement used in automatic assertion +intersection_of: ECO:0000033 ! author statement supported by traceable reference +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007322 +name: curator inference used in automatic assertion +def: "A type of curator inference that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000205 {is_inferred="true"} ! curator inference +is_a: ECO:0007832 {is_inferred="true"} ! inferential evidence used in automatic assertion +intersection_of: ECO:0000205 ! curator inference +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007323 +name: inference from background scientific knowledge used in automatic assertion +def: "A type of inference from background scientific knowledge that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000001 {is_inferred="true"} ! inference from background scientific knowledge +is_a: ECO:0007322 {is_inferred="true"} ! curator inference used in automatic assertion +intersection_of: ECO:0000001 ! inference from background scientific knowledge +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007324 +name: no evidence data found used in automatic assertion +def: "An inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000035 {is_inferred="true"} ! no evidence data found +is_a: ECO:0007322 {is_inferred="true"} ! curator inference used in automatic assertion +intersection_of: ECO:0000035 ! no evidence data found +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007325 +name: mutant phenotype evidence used in automatic assertion +def: "A type of mutant phenotype evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000015 {is_inferred="true"} ! mutant phenotype evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0000015 ! mutant phenotype evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007326 +name: genetic interaction evidence used in automatic assertion +def: "A type of genetic interaction evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000011 {is_inferred="true"} ! genetic interaction evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0000011 ! genetic interaction evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007327 +name: genomic context evidence used in automatic assertion +def: "A type of genomic context evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000177 {is_inferred="true"} ! genomic context evidence +is_a: ECO:0000251 {is_inferred="true"} ! similarity evidence used in automatic assertion +intersection_of: ECO:0000177 ! genomic context evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007328 +name: biological aspect of ancestor evidence used in automatic assertion +def: "A type of biological aspect of ancestor evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000308 {is_inferred="true"} ! biological aspect of ancestor evidence +is_a: ECO:0007826 {is_inferred="true"} ! phylogenetic evidence used in automatic assertion +intersection_of: ECO:0000308 ! biological aspect of ancestor evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007329 +name: biological aspect of descendant evidence used in automatic assertion +def: "A type of biological aspect of descendant evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000214 {is_inferred="true"} ! biological aspect of descendant evidence +is_a: ECO:0007826 {is_inferred="true"} ! phylogenetic evidence used in automatic assertion +intersection_of: ECO:0000214 ! biological aspect of descendant evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007330 +name: phylogenetic determination of loss of key residues evidence used in automatic assertion +def: "A type of phylogenetic determination of loss of key residues evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000216 {is_inferred="true"} ! phylogenetic determination of loss of key residues evidence +is_a: ECO:0007826 {is_inferred="true"} ! phylogenetic evidence used in automatic assertion +intersection_of: ECO:0000216 ! phylogenetic determination of loss of key residues evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007331 +name: rapid divergence from ancestral sequence evidence used in automatic assertion +def: "A type of rapid divergence from ancestral sequence evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000215 {is_inferred="true"} ! rapid divergence from ancestral sequence evidence +is_a: ECO:0007826 {is_inferred="true"} ! phylogenetic evidence used in automatic assertion +intersection_of: ECO:0000215 ! rapid divergence from ancestral sequence evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007332 +name: physical interaction evidence used in automatic assertion +def: "A type of physical interaction evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000021 {is_inferred="true"} ! physical interaction evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000021 ! physical interaction evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007333 +name: gene neighbors evidence used in automatic assertion +def: "A type of gene neighbors evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000084 {is_inferred="true"} ! gene neighbors evidence +is_a: ECO:0007327 {is_inferred="true"} ! genomic context evidence used in automatic assertion +intersection_of: ECO:0000084 ! gene neighbors evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007334 +name: Edman degradation evidence used in automatic assertion +def: "A type of Edman degradation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001044 {is_inferred="true"} ! Edman degradation evidence +is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion +intersection_of: ECO:0001044 ! Edman degradation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007335 +name: 3D cell culture evidence used in automatic assertion +def: "A type of 3D cell culture evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001001 {is_inferred="true"} ! 3D cell culture evidence +is_a: ECO:0007775 {is_inferred="true"} ! cell proliferation assay evidence used in automatic assertion +intersection_of: ECO:0001001 ! 3D cell culture evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007336 +name: 51Cr release assay evidence used in automatic assertion +def: "A type of 51Cr release assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001004 {is_inferred="true"} ! 51Cr release assay evidence +is_a: ECO:0007781 {is_inferred="true"} ! cytotoxicity assay evidence used in automatic assertion +intersection_of: ECO:0001004 ! 51Cr release assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007337 +name: 7-aminoactinomycin staining evidence used in automatic assertion +def: "A type of 7-aminoactinomycin staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001005 {is_inferred="true"} ! 7-aminoactinomycin staining evidence +is_a: ECO:0007798 {is_inferred="true"} ! staining evidence used in automatic assertion +intersection_of: ECO:0001005 ! 7-aminoactinomycin staining evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007338 +name: [3H]-thymidine incorporation assay evidence used in automatic assertion +def: "A type of [3H]-thymidine incorporation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001003 {is_inferred="true"} ! [3H]-thymidine incorporation assay evidence +is_a: ECO:0007776 {is_inferred="true"} ! DNA synthesis cell proliferation assay evidence used in automatic assertion +intersection_of: ECO:0001003 ! [3H]-thymidine incorporation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007339 +name: [3H]arachidonic acid release assay evidence used in automatic assertion +def: "A type of [3H]arachidonic acid release assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001002 {is_inferred="true"} ! [3H]arachidonic acid release assay evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0001002 ! [3H]arachidonic acid release assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007340 +name: adhesion assay evidence used in automatic assertion +def: "A type of adhesion assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001006 {is_inferred="true"} ! adhesion assay evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0001006 ! adhesion assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007341 +name: adoptive cell transfer evidence used in automatic assertion +def: "A type of adoptive cell transfer evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001007 {is_inferred="true"} ! adoptive cell transfer evidence +is_a: ECO:0007793 {is_inferred="true"} ! ex vivo assay evidence used in automatic assertion +intersection_of: ECO:0001007 ! adoptive cell transfer evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007342 +name: alamarBlue assay evidence used in automatic assertion +def: "A type of alamarBlue assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001008 {is_inferred="true"} ! alamarBlue assay evidence +is_a: ECO:0007774 {is_inferred="true"} ! cell viability assay evidence used in automatic assertion +intersection_of: ECO:0001008 ! alamarBlue assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007343 +name: allograft transplantation phenotypic evidence used in automatic assertion +def: "A type of allograft transplantation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "allograft transplantation evidence used in automatic assertion" EXACT [] +is_a: ECO:0001009 {is_inferred="true"} ! allograft transplantation phenotypic evidence +is_a: ECO:0007810 {is_inferred="true"} ! anatomical perturbation phenotypic evidence used in automatic assertion +intersection_of: ECO:0001009 ! allograft transplantation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007344 +name: anion-exchange chromatography evidence used in automatic assertion +def: "A type of anion-exchange chromatography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001010 {is_inferred="true"} ! anion-exchange chromatography evidence +is_a: ECO:0007681 {is_inferred="true"} ! chromatography evidence used in automatic assertion +intersection_of: ECO:0001010 ! anion-exchange chromatography evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007345 +name: annexin-V staining evidence used in automatic assertion +def: "A type of annexin-V staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001011 {is_inferred="true"} ! annexin-V staining evidence +is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion +intersection_of: ECO:0001011 ! annexin-V staining evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007346 +name: cognitive assay phenotypic evidence used in automatic assertion +def: "A type of cognitive assay phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "behavioral assay evidence used in automatic assertion" EXACT [] +is_a: ECO:0001012 {is_inferred="true"} ! cognitive assay phenotypic evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0001012 ! cognitive assay phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007347 +name: blocking monoclonal antibody evidence used in automatic assertion +def: "A type of blocking monoclonal antibody evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001013 {is_inferred="true"} ! blocking monoclonal antibody evidence +is_a: ECO:0007348 {is_inferred="true"} ! immunological assay evidence used in automatic assertion +intersection_of: ECO:0001013 ! blocking monoclonal antibody evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007348 +name: immunological assay evidence used in automatic assertion +def: "A type of immunological assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000040 {is_inferred="true"} ! immunological assay evidence +is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion +intersection_of: ECO:0000040 ! immunological assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007349 +name: blocking peptide evidence used in automatic assertion +def: "A type of blocking peptide evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001014 {is_inferred="true"} ! blocking peptide evidence +is_a: ECO:0007348 {is_inferred="true"} ! immunological assay evidence used in automatic assertion +intersection_of: ECO:0001014 ! blocking peptide evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007350 +name: blocking polyclonal antibody evidence used in automatic assertion +def: "A type of blocking polyclonal antibody evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001015 {is_inferred="true"} ! blocking polyclonal antibody evidence +is_a: ECO:0007348 {is_inferred="true"} ! immunological assay evidence used in automatic assertion +intersection_of: ECO:0001015 ! blocking polyclonal antibody evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007351 +name: blood test evidence used in automatic assertion +def: "A type of blood test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001016 {is_inferred="true"} ! blood test evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001016 ! blood test evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007352 +name: Boyden chamber assay evidence used in automatic assertion +def: "A type of Boyden chamber assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001017 {is_inferred="true"} ! Boyden chamber assay evidence +is_a: ECO:0007780 {is_inferred="true"} ! chemotaxis assay evidence used in automatic assertion +intersection_of: ECO:0001017 ! Boyden chamber assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007353 +name: bromodeoxyuridine incorporation assay evidence used in automatic assertion +def: "A type of bromodeoxyuridine incorporation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001018 {is_inferred="true"} ! bromodeoxyuridine incorporation assay evidence +is_a: ECO:0007354 {is_inferred="true"} ! nucleotide analog incorporation assay evidence used in automatic assertion +intersection_of: ECO:0001018 ! bromodeoxyuridine incorporation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007354 +name: nucleotide analog incorporation assay evidence used in automatic assertion +def: "A type of nucleotide analog incorporation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001107 {is_inferred="true"} ! nucleotide analog incorporation assay evidence +is_a: ECO:0007776 {is_inferred="true"} ! DNA synthesis cell proliferation assay evidence used in automatic assertion +intersection_of: ECO:0001107 ! nucleotide analog incorporation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007355 +name: caspase assay evidence used in automatic assertion +def: "A type of caspase assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001019 {is_inferred="true"} ! caspase assay evidence +is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion +intersection_of: ECO:0001019 ! caspase assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007356 +name: cell counting evidence used in automatic assertion +def: "A type of cell counting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001020 {is_inferred="true"} ! cell counting evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0001020 ! cell counting evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007357 +name: cell permeability assay evidence used in automatic assertion +def: "A type of cell permeability assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001021 {is_inferred="true"} ! cell permeability assay evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0001021 ! cell permeability assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007358 +name: carboxyfluorescein diacetate succinimidyl ester staining evidence used in automatic assertion +def: "A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001022 {is_inferred="true"} ! carboxyfluorescein diacetate succinimidyl ester staining evidence +is_a: ECO:0007798 {is_inferred="true"} ! staining evidence used in automatic assertion +intersection_of: ECO:0001022 ! carboxyfluorescein diacetate succinimidyl ester staining evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007359 +name: chemiluminescence-linked immunoassay evidence used in automatic assertion +def: "A type of chemiluminescence-linked immunoassay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001023 {is_inferred="true"} ! chemiluminescence-linked immunoassay evidence +is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion +intersection_of: ECO:0001023 ! chemiluminescence-linked immunoassay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007360 +name: chimeric protein phenotypic evidence used in automatic assertion +def: "A type of chimeric protein phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "chimeric protein evidence used in automatic assertion" EXACT [] +is_a: ECO:0001024 {is_inferred="true"} ! chimeric protein phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0001024 ! chimeric protein phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007361 +name: co-electrophoresis evidence used in automatic assertion +def: "A type of co-electrophoresis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001025 {is_inferred="true"} ! co-electrophoresis evidence +is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion +intersection_of: ECO:0001025 ! co-electrophoresis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007362 +name: co-localization evidence used in automatic assertion +def: "A type of co-localization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001026 {is_inferred="true"} ! co-localization evidence +is_a: ECO:0007363 {is_inferred="true"} ! imaging assay evidence used in automatic assertion +intersection_of: ECO:0001026 ! co-localization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007363 +name: imaging assay evidence used in automatic assertion +def: "A type of imaging assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000324 {is_inferred="true"} ! imaging assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000324 ! imaging assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007364 +name: co-sedimentation assay evidence used in automatic assertion +def: "A type of co-sedimentation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001028 {is_inferred="true"} ! co-sedimentation assay evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +intersection_of: ECO:0001028 ! co-sedimentation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007365 +name: colony counting evidence used in automatic assertion +def: "A type of colony counting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001027 {is_inferred="true"} ! colony counting evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001027 ! colony counting evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007366 +name: comet assay evidence used in automatic assertion +def: "A type of comet assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001029 {is_inferred="true"} ! comet assay evidence +is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion +intersection_of: ECO:0001029 ! comet assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007367 +name: conditional knockin evidence used in automatic assertion +def: "A type of conditional knockin evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001031 {is_inferred="true"} ! conditional knockin evidence +is_a: ECO:0007368 {is_inferred="true"} ! knockin evidence used in automatic assertion +intersection_of: ECO:0001031 ! conditional knockin evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007368 +name: knockin evidence used in automatic assertion +def: "A type of knockin evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001090 {is_inferred="true"} ! knockin evidence +is_a: ECO:0007427 {is_inferred="true"} ! genetic transformation evidence used in automatic assertion +intersection_of: ECO:0001090 ! knockin evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007369 +name: conditional knockout evidence used in automatic assertion +def: "A type of conditional knockout evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001030 {is_inferred="true"} ! conditional knockout evidence +is_a: ECO:0007370 {is_inferred="true"} ! knockout evidence used in automatic assertion +intersection_of: ECO:0001030 ! conditional knockout evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007370 +name: knockout evidence used in automatic assertion +def: "A type of knockout evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001091 {is_inferred="true"} ! knockout phenotypic evidence +is_a: ECO:0007427 {is_inferred="true"} ! genetic transformation evidence used in automatic assertion +intersection_of: ECO:0001091 ! knockout phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007371 +name: constitutively active mutant evidence used in automatic assertion +def: "A type of constitutively active mutant evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001032 {is_inferred="true"} ! constitutively active mutant evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0001032 ! constitutively active mutant evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007372 +name: cross-linking evidence used in automatic assertion +def: "A type of cross-linking evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001033 {is_inferred="true"} ! cross-linking evidence +is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion +intersection_of: ECO:0001033 ! cross-linking evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007373 +name: protein binding evidence used in automatic assertion +def: "A type of protein binding evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000024 {is_inferred="true"} ! protein binding evidence +is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion +intersection_of: ECO:0000024 ! protein binding evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007374 +name: crystallography evidence used in automatic assertion +def: "A type of crystallography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001034 {is_inferred="true"} ! crystallography evidence +is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion +intersection_of: ECO:0001034 ! crystallography evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007375 +name: cytochemistry evidence used in automatic assertion +def: "A type of cytochemistry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001035 {is_inferred="true"} ! cytochemistry evidence +is_a: ECO:0007376 {is_inferred="true"} ! histochemistry evidence used in automatic assertion +intersection_of: ECO:0001035 ! cytochemistry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007376 +name: histochemistry evidence used in automatic assertion +def: "A type of histochemistry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001050 {is_inferred="true"} ! histochemistry evidence +is_a: ECO:0007389 {is_inferred="true"} ! histology evidence used in automatic assertion +intersection_of: ECO:0001050 ! histochemistry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007377 +name: cytochrome C release assay evidence used in automatic assertion +def: "A type of cytochrome C release assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001036 {is_inferred="true"} ! cytochrome C release assay evidence +is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion +intersection_of: ECO:0001036 ! cytochrome C release assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007378 +name: 4',6-diamidino-2-phenylindole staining evidence used in automatic assertion +def: "A type of 4',6-diamidino-2-phenylindole staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001037 {is_inferred="true"} ! 4',6-diamidino-2-phenylindole staining evidence +is_a: ECO:0007798 {is_inferred="true"} ! staining evidence used in automatic assertion +intersection_of: ECO:0001037 ! 4',6-diamidino-2-phenylindole staining evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007379 +name: deletion mutation phenotypic evidence used in automatic assertion +def: "A type of deletion mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "deletion mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0001038 {is_inferred="true"} ! deletion mutation phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0001038 ! deletion mutation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007380 +name: DNA laddering assay evidence used in automatic assertion +def: "A type of DNA laddering assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001039 {is_inferred="true"} ! DNA laddering assay evidence +is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion +intersection_of: ECO:0001039 ! DNA laddering assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007381 +name: RNA dot blot assay evidence used in automatic assertion +def: "A type of RNA dot blot assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001040 {is_inferred="true"} ! RNA dot blot assay evidence +is_a: ECO:0007818 {is_inferred="true"} ! RNA detection assay evidence used in automatic assertion +intersection_of: ECO:0001040 ! RNA dot blot assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007382 +name: dominant-negative mutant phenotypic evidence used in automatic assertion +def: "A type of dominant-negative mutant phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "dominant-negative mutant evidence used in automatic assertion" EXACT [] +is_a: ECO:0001042 {is_inferred="true"} ! dominant-negative mutant phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0001042 ! dominant-negative mutant phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007383 +name: eTag assay evidence used in automatic assertion +def: "A type of eTag assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001045 {is_inferred="true"} ! eTag assay evidence +is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion +intersection_of: ECO:0001045 ! eTag assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007384 +name: affinity evidence used in automatic assertion +def: "A type of affinity evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000023 {is_inferred="true"} ! affinity evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +intersection_of: ECO:0000023 ! affinity evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007385 +name: filter binding assay evidence used in automatic assertion +def: "A type of filter binding assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001046 {is_inferred="true"} ! filter binding assay evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +intersection_of: ECO:0001046 ! filter binding assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007386 +name: fluorescence in situ hybridization evidence used in automatic assertion +def: "A type of fluorescence in situ hybridization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001047 {is_inferred="true"} ! fluorescence in situ hybridization evidence +is_a: ECO:0001837 {is_inferred="true"} ! in situ hybridization evidence used in automatic assertion +is_a: ECO:0007809 {is_inferred="true"} ! nucleic acid localization evidence used in automatic assertion +intersection_of: ECO:0001047 ! fluorescence in situ hybridization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007387 +name: fluorescence resonance energy transfer evidence used in automatic assertion +def: "A type of fluorescence resonance energy transfer evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001048 {is_inferred="true"} ! fluorescence resonance energy transfer evidence +is_a: ECO:0006328 {is_inferred="true"} ! dynamic fluorescence quenching evidence used in automatic assertion +intersection_of: ECO:0001048 ! fluorescence resonance energy transfer evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007388 +name: gel-filtration evidence used in automatic assertion +def: "A type of gel-filtration evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001049 {is_inferred="true"} ! gel-filtration evidence +is_a: ECO:0007681 {is_inferred="true"} ! chromatography evidence used in automatic assertion +intersection_of: ECO:0001049 ! gel-filtration evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007389 +name: histology evidence used in automatic assertion +def: "A type of histology evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001051 {is_inferred="true"} ! histology evidence +is_a: ECO:0007363 {is_inferred="true"} ! imaging assay evidence used in automatic assertion +intersection_of: ECO:0001051 ! histology evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007390 +name: immunocytochemistry evidence used in automatic assertion +def: "A type of immunocytochemistry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001053 {is_inferred="true"} ! immunocytochemistry evidence +is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion +intersection_of: ECO:0001053 ! immunocytochemistry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007391 +name: immunodepletion evidence used in automatic assertion +def: "A type of immunodepletion evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001054 {is_inferred="true"} ! immunodepletion evidence +is_a: ECO:0007348 {is_inferred="true"} ! immunological assay evidence used in automatic assertion +intersection_of: ECO:0001054 ! immunodepletion evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007392 +name: immunohistochemistry evidence used in automatic assertion +def: "A type of immunohistochemistry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001055 {is_inferred="true"} ! immunohistochemistry evidence +is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion +intersection_of: ECO:0001055 ! immunohistochemistry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007393 +name: in vitro acetylation assay evidence used in automatic assertion +def: "A type of in vitro acetylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001057 {is_inferred="true"} ! in vitro acetylation assay evidence +is_a: ECO:0007794 {is_inferred="true"} ! acetylation assay evidence used in automatic assertion +intersection_of: ECO:0001057 ! in vitro acetylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007394 +name: in vitro cleavage assay evidence used in automatic assertion +def: "A type of in vitro cleavage assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001058 {is_inferred="true"} ! in vitro cleavage assay evidence +is_a: ECO:0007782 {is_inferred="true"} ! cleavage assay evidence used in automatic assertion +intersection_of: ECO:0001058 ! in vitro cleavage assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007395 +name: in vitro deacetylation assay evidence used in automatic assertion +def: "A type of in vitro deacetylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001060 {is_inferred="true"} ! in vitro deacetylation assay evidence +is_a: ECO:0007771 {is_inferred="true"} ! deacetylation assay evidence used in automatic assertion +intersection_of: ECO:0001060 ! in vitro deacetylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007396 +name: in vitro defarnesylation assay evidence used in automatic assertion +def: "A type of in vitro defarnesylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001061 {is_inferred="true"} ! in vitro defarnesylation assay evidence +is_a: ECO:0007790 {is_inferred="true"} ! defarnesylation assay evidence used in automatic assertion +intersection_of: ECO:0001061 ! in vitro defarnesylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007397 +name: in vitro demethylation assay evidence used in automatic assertion +def: "A type of in vitro demethylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001062 {is_inferred="true"} ! in vitro demethylation assay evidence +is_a: ECO:0007795 {is_inferred="true"} ! demethylation assay evidence used in automatic assertion +intersection_of: ECO:0001062 ! in vitro demethylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007398 +name: in vitro desumoylation assay evidence used in automatic assertion +def: "A type of in vitro desumoylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001063 {is_inferred="true"} ! in vitro desumoylation assay evidence +is_a: ECO:0007802 {is_inferred="true"} ! desumoylation assay evidence used in automatic assertion +intersection_of: ECO:0001063 ! in vitro desumoylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007399 +name: in vitro deubiquitination assay evidence used in automatic assertion +def: "A type of in vitro deubiquitination assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001059 {is_inferred="true"} ! in vitro deubiquitination assay evidence +is_a: ECO:0007791 {is_inferred="true"} ! deubiquitination assay evidence used in automatic assertion +intersection_of: ECO:0001059 ! in vitro deubiquitination assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007400 +name: in vitro farnesylation assay evidence used in automatic assertion +def: "A type of in vitro farnesylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001064 {is_inferred="true"} ! in vitro farnesylation assay evidence +is_a: ECO:0007800 {is_inferred="true"} ! farnesylation assay evidence used in automatic assertion +intersection_of: ECO:0001064 ! in vitro farnesylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007401 +name: in vitro methylation assay evidence used in automatic assertion +def: "A type of in vitro methylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001065 {is_inferred="true"} ! in vitro methylation assay evidence +is_a: ECO:0007783 {is_inferred="true"} ! methylation assay evidence used in automatic assertion +intersection_of: ECO:0001065 ! in vitro methylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007402 +name: in vitro palmitoylation assay evidence used in automatic assertion +def: "A type of in vitro palmitoylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001066 {is_inferred="true"} ! in vitro palmitoylation assay evidence +is_a: ECO:0007792 {is_inferred="true"} ! palmitoylation assay evidence used in automatic assertion +intersection_of: ECO:0001066 ! in vitro palmitoylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007403 +name: in vitro phosphatase assay evidence used in automatic assertion +def: "A type of in vitro phosphatase assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001067 {is_inferred="true"} ! in vitro phosphatase assay evidence +is_a: ECO:0007772 {is_inferred="true"} ! phosphatase assay evidence used in automatic assertion +intersection_of: ECO:0001067 ! in vitro phosphatase assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007404 +name: in vitro polyADP-ribosylation assay evidence used in automatic assertion +def: "A type of in vitro polyADP-ribosylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001069 {is_inferred="true"} ! in vitro polyADP-ribosylation assay evidence +is_a: ECO:0007797 {is_inferred="true"} ! polyADP-ribosylation assay evidence used in automatic assertion +intersection_of: ECO:0001069 ! in vitro polyADP-ribosylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007405 +name: in vitro protein kinase assay evidence used in automatic assertion +def: "A type of in vitro protein kinase assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001068 {is_inferred="true"} ! in vitro protein kinase assay evidence +is_a: ECO:0007767 {is_inferred="true"} ! protein kinase assay evidence used in automatic assertion +intersection_of: ECO:0001068 ! in vitro protein kinase assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007406 +name: in vitro sumoylation assay evidence used in automatic assertion +def: "A type of in vitro sumoylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001070 {is_inferred="true"} ! in vitro sumoylation assay evidence +is_a: ECO:0007788 {is_inferred="true"} ! sumoylation assay evidence used in automatic assertion +intersection_of: ECO:0001070 ! in vitro sumoylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007407 +name: in vitro transcription assay evidence used in automatic assertion +def: "A type of in vitro transcription assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001071 {is_inferred="true"} ! in vitro transcription assay evidence +is_a: ECO:0007768 {is_inferred="true"} ! transcription assay evidence used in automatic assertion +intersection_of: ECO:0001071 ! in vitro transcription assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007408 +name: in vitro translation assay evidence used in automatic assertion +def: "A type of in vitro translation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001072 {is_inferred="true"} ! in vitro translation assay evidence +is_a: ECO:0007799 {is_inferred="true"} ! translation assay evidence used in automatic assertion +intersection_of: ECO:0001072 ! in vitro translation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007409 +name: in vitro ubiquitination assay evidence used in automatic assertion +def: "A type of in vitro ubiquitination assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001073 {is_inferred="true"} ! in vitro ubiquitination assay evidence +is_a: ECO:0007801 {is_inferred="true"} ! ubiquitination assay evidence used in automatic assertion +intersection_of: ECO:0001073 ! in vitro ubiquitination assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007410 +name: in vivo acetylation assay evidence used in automatic assertion +def: "A type of in vivo acetylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001074 {is_inferred="true"} ! in vivo acetylation assay evidence +is_a: ECO:0007794 {is_inferred="true"} ! acetylation assay evidence used in automatic assertion +intersection_of: ECO:0001074 ! in vivo acetylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007411 +name: in vivo cleavage assay evidence used in automatic assertion +def: "A type of in vivo cleavage assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001075 {is_inferred="true"} ! in vivo cleavage assay evidence +is_a: ECO:0007782 {is_inferred="true"} ! cleavage assay evidence used in automatic assertion +intersection_of: ECO:0001075 ! in vivo cleavage assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007412 +name: in vivo deacetylation assay evidence used in automatic assertion +def: "A type of in vivo deacetylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001076 {is_inferred="true"} ! in vivo deacetylation assay evidence +is_a: ECO:0007771 {is_inferred="true"} ! deacetylation assay evidence used in automatic assertion +intersection_of: ECO:0001076 ! in vivo deacetylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007413 +name: in vivo defarnesylation assay evidence used in automatic assertion +def: "A type of in vivo defarnesylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001077 {is_inferred="true"} ! in vivo defarnesylation assay evidence +is_a: ECO:0007790 {is_inferred="true"} ! defarnesylation assay evidence used in automatic assertion +intersection_of: ECO:0001077 ! in vivo defarnesylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007414 +name: in vivo demethylation assay evidence used in automatic assertion +def: "A type of in vivo demethylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001078 {is_inferred="true"} ! in vivo demethylation assay evidence +is_a: ECO:0007795 {is_inferred="true"} ! demethylation assay evidence used in automatic assertion +intersection_of: ECO:0001078 ! in vivo demethylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007415 +name: in vivo desumoylation assay evidence used in automatic assertion +def: "A type of in vivo desumoylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001080 {is_inferred="true"} ! in vivo desumoylation assay evidence +is_a: ECO:0007802 {is_inferred="true"} ! desumoylation assay evidence used in automatic assertion +intersection_of: ECO:0001080 ! in vivo desumoylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007416 +name: in vivo deubiquitination assay evidence used in automatic assertion +def: "A type of in vivo deubiquitination assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001079 {is_inferred="true"} ! in vivo deubiquitination assay evidence +is_a: ECO:0007791 {is_inferred="true"} ! deubiquitination assay evidence used in automatic assertion +intersection_of: ECO:0001079 ! in vivo deubiquitination assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007417 +name: in vivo farnesylation assay evidence used in automatic assertion +def: "A type of in vivo farnesylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001081 {is_inferred="true"} ! in vivo farnesylation assay evidence +is_a: ECO:0007800 {is_inferred="true"} ! farnesylation assay evidence used in automatic assertion +intersection_of: ECO:0001081 ! in vivo farnesylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007418 +name: in vivo methylation assay evidence used in automatic assertion +def: "A type of in vivo methylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001082 {is_inferred="true"} ! in vivo methylation assay evidence +is_a: ECO:0007783 {is_inferred="true"} ! methylation assay evidence used in automatic assertion +intersection_of: ECO:0001082 ! in vivo methylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007419 +name: in vivo palmitoylation assay evidence used in automatic assertion +def: "A type of in vivo palmitoylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001083 {is_inferred="true"} ! in vivo palmitoylation assay evidence +is_a: ECO:0007792 {is_inferred="true"} ! palmitoylation assay evidence used in automatic assertion +intersection_of: ECO:0001083 ! in vivo palmitoylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007420 +name: in vivo phosphatase assay evidence used in automatic assertion +def: "A type of in vivo phosphatase assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001084 {is_inferred="true"} ! in vivo phosphatase assay evidence +is_a: ECO:0007772 {is_inferred="true"} ! phosphatase assay evidence used in automatic assertion +intersection_of: ECO:0001084 ! in vivo phosphatase assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007421 +name: in vivo protein kinase assay evidence used in automatic assertion +def: "A type of in vivo protein kinase assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001085 {is_inferred="true"} ! in vivo protein kinase assay evidence +is_a: ECO:0007767 {is_inferred="true"} ! protein kinase assay evidence used in automatic assertion +intersection_of: ECO:0001085 ! in vivo protein kinase assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007422 +name: in vivo sumoylation assay evidence used in automatic assertion +def: "A type of in vivo sumoylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001086 {is_inferred="true"} ! in vivo sumoylation assay evidence +is_a: ECO:0007788 {is_inferred="true"} ! sumoylation assay evidence used in automatic assertion +intersection_of: ECO:0001086 ! in vivo sumoylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007423 +name: in vivo transcription assay evidence used in automatic assertion +def: "A type of in vivo transcription assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001087 {is_inferred="true"} ! in vivo transcription assay evidence +is_a: ECO:0007768 {is_inferred="true"} ! transcription assay evidence used in automatic assertion +intersection_of: ECO:0001087 ! in vivo transcription assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007424 +name: in vivo translation assay evidence used in automatic assertion +def: "A type of in vivo translation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001088 {is_inferred="true"} ! in vivo translation assay evidence +is_a: ECO:0007799 {is_inferred="true"} ! translation assay evidence used in automatic assertion +intersection_of: ECO:0001088 ! in vivo translation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007425 +name: in vivo ubiquitination assay evidence used in automatic assertion +def: "A type of in vivo ubiquitination assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001089 {is_inferred="true"} ! in vivo ubiquitination assay evidence +is_a: ECO:0007801 {is_inferred="true"} ! ubiquitination assay evidence used in automatic assertion +intersection_of: ECO:0001089 ! in vivo ubiquitination assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007426 +name: induced mutation evidence used in automatic assertion +def: "A type of induced mutation evidence that is used in an automatic assertion." [ECO:RCT] +is_obsolete: true +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007427 +name: genetic transformation evidence used in automatic assertion +def: "A type of genetic transformation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005027 {is_inferred="true"} ! genetic transformation evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0005027 ! genetic transformation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007428 +name: lipid binding assay evidence used in automatic assertion +def: "A type of lipid binding assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001092 {is_inferred="true"} ! lipid binding assay evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +intersection_of: ECO:0001092 ! lipid binding assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007429 +name: luminescence-based mammalian interactome mapping assay evidence used in automatic assertion +def: "A type of luminescence-based mammalian interactome mapping assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001093 {is_inferred="true"} ! luminescence-based mammalian interactome mapping assay evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +intersection_of: ECO:0001093 ! luminescence-based mammalian interactome mapping assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007430 +name: macroscopy evidence used in automatic assertion +def: "A type of macroscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001094 {is_inferred="true"} ! macroscopy evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0001094 ! macroscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007431 +name: mammalian 2-hybrid assay evidence used in automatic assertion +def: "A type of mammalian 2-hybrid assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001095 {is_inferred="true"} ! mammalian 2-hybrid assay evidence +is_a: ECO:0007432 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in automatic assertion +intersection_of: ECO:0001095 ! mammalian 2-hybrid assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007432 +name: bait-prey hybrid interaction evidence used in automatic assertion +def: "A type of bait-prey hybrid interaction evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "bait-prey protein pull-down evidence" EXACT [] +is_a: ECO:0000025 {is_inferred="true"} ! bait-prey hybrid interaction evidence +is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion +intersection_of: ECO:0000025 ! bait-prey hybrid interaction evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007433 +name: mass spectrometry evidence used in automatic assertion +def: "A type of mass spectrometry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001096 {is_inferred="true"} ! mass spectrometry evidence +is_a: ECO:0007821 {is_inferred="true"} ! spectrometry evidence used in automatic assertion +intersection_of: ECO:0001096 ! mass spectrometry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007434 +name: medical imaging evidence used in automatic assertion +def: "A type of medical imaging evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001097 {is_inferred="true"} ! medical imaging evidence +is_a: ECO:0007363 {is_inferred="true"} ! imaging assay evidence used in automatic assertion +intersection_of: ECO:0001097 ! medical imaging evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007435 +name: microscopy evidence used in automatic assertion +def: "A type of microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001098 {is_inferred="true"} ! microscopy evidence +is_a: ECO:0007363 {is_inferred="true"} ! imaging assay evidence used in automatic assertion +intersection_of: ECO:0001098 ! microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007436 +name: motility wound healing assay evidence used in automatic assertion +def: "A type of motility wound healing assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001099 {is_inferred="true"} ! motility wound healing assay evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0001099 ! motility wound healing assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007437 +name: MTS assay evidence used in automatic assertion +def: "A type of MTS assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001100 {is_inferred="true"} ! MTS assay evidence +is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion +intersection_of: ECO:0001100 ! MTS assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007438 +name: MTT assay evidence used in automatic assertion +def: "A type of MTT assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001101 {is_inferred="true"} ! MTT assay evidence +is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion +intersection_of: ECO:0001101 ! MTT assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007439 +name: multiplex bead-based immunoassay evidence used in automatic assertion +def: "A type of multiplex bead-based immunoassay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001102 {is_inferred="true"} ! multiplex bead-based immunoassay evidence +is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion +intersection_of: ECO:0001102 ! multiplex bead-based immunoassay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007440 +name: natural variation mutant evidence used in automatic assertion +def: "A type of natural variation mutant evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001103 {is_inferred="true"} ! natural variation mutant evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0001103 ! natural variation mutant evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007441 +name: nuclear magnetic resonance evidence used in automatic assertion +def: "A type of nuclear magnetic resonance evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001105 {is_inferred="true"} ! nuclear magnetic resonance evidence +is_a: ECO:0006286 {is_inferred="true"} ! magnetic resonance evidence used in automatic assertion +intersection_of: ECO:0001105 ! nuclear magnetic resonance evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007442 +name: nuclear fragmentation evidence used in automatic assertion +def: "A type of nuclear fragmentation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001104 {is_inferred="true"} ! nuclear fragmentation evidence +is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion +intersection_of: ECO:0001104 ! nuclear fragmentation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007443 +name: phage display evidence used in automatic assertion +def: "A type of phage display evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001108 {is_inferred="true"} ! phage display evidence +is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion +intersection_of: ECO:0001108 ! phage display evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007444 +name: phosphoamino acid analysis evidence used in automatic assertion +def: "A type of phosphoamino acid analysis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001109 {is_inferred="true"} ! phosphoamino acid analysis evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001109 ! phosphoamino acid analysis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007445 +name: peptide affinity enrichment evidence used in automatic assertion +def: "A type of peptide affinity enrichment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001110 {is_inferred="true"} ! peptide affinity enrichment evidence +is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion +intersection_of: ECO:0001110 ! peptide affinity enrichment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007446 +name: peptide array evidence used in automatic assertion +def: "A type of peptide array evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001112 {is_inferred="true"} ! peptide array evidence +is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion +intersection_of: ECO:0001112 ! peptide array evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007447 +name: physical examination evidence used in automatic assertion +def: "A type of physical examination evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001111 {is_inferred="true"} ! physical examination evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001111 ! physical examination evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007448 +name: point mutation phenotypic evidence used in automatic assertion +def: "A type of point mutation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "point mutation evidence used in automatic assertion" EXACT [] +is_a: ECO:0001113 {is_inferred="true"} ! point mutation phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0001113 ! point mutation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007449 +name: propidium iodide staining evidence used in automatic assertion +def: "A type of propidium iodide staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001114 {is_inferred="true"} ! propidium iodide staining evidence +is_a: ECO:0007798 {is_inferred="true"} ! staining evidence used in automatic assertion +intersection_of: ECO:0001114 ! propidium iodide staining evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007450 +name: fluorescence evidence used in automatic assertion +def: "A type of fluorescence evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001115 {is_inferred="true"} ! fluorescence evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001115 ! fluorescence evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007451 +name: protein dot blot assay evidence used in automatic assertion +def: "A type of protein dot blot assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001116 {is_inferred="true"} ! protein dot blot assay evidence +is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion +intersection_of: ECO:0001116 ! protein dot blot assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007452 +name: protein microarray evidence used in automatic assertion +def: "A type of protein microarray evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001117 {is_inferred="true"} ! protein microarray evidence +is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion +intersection_of: ECO:0001117 ! protein microarray evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007453 +name: protein sequencing assay evidence used in automatic assertion +def: "A type of protein sequencing assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001118 {is_inferred="true"} ! protein sequencing assay evidence +is_a: ECO:0007812 {is_inferred="true"} ! sequencing assay evidence used in automatic assertion +intersection_of: ECO:0001118 ! protein sequencing assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007454 +name: quantitative mass spectrometry evidence used in automatic assertion +def: "A type of quantitative mass spectrometry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001119 {is_inferred="true"} ! quantitative mass spectrometry evidence +is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion +intersection_of: ECO:0001119 ! quantitative mass spectrometry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007455 +name: radioisotope assay evidence used in automatic assertion +def: "A type of radioisotope assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001120 {is_inferred="true"} ! radioisotope assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001120 ! radioisotope assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007456 +name: radioimmunoassay evidence used in automatic assertion +def: "A type of radioimmunoassay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001121 {is_inferred="true"} ! radioimmunoassay evidence +is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion +intersection_of: ECO:0001121 ! radioimmunoassay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007457 +name: restriction fragment detection evidence used in automatic assertion +def: "A type of restriction fragment detection evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001124 {is_inferred="true"} ! restriction fragment detection evidence +is_a: ECO:0007817 {is_inferred="true"} ! DNA detection assay evidence used in automatic assertion +intersection_of: ECO:0001124 ! restriction fragment detection evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007458 +name: spectrophotometry evidence used in automatic assertion +def: "A type of spectrophotometry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001126 {is_inferred="true"} ! spectrophotometry evidence +is_a: ECO:0007821 {is_inferred="true"} ! spectrometry evidence used in automatic assertion +intersection_of: ECO:0001126 ! spectrophotometry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007459 +name: syngeneic transplantation experiment evidence used in automatic assertion +def: "A type of syngeneic transplantation experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001128 {is_inferred="true"} ! syngeneic transplantation experiment evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0001128 ! syngeneic transplantation experiment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007460 +name: xenotransplantation phenotypic evidence used in automatic assertion +def: "A type of xenotransplantation phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "xenotransplantation experiment evidence used in automatic assertion" EXACT [] +is_a: ECO:0001137 {is_inferred="true"} ! xenotransplantation phenotypic evidence +is_a: ECO:0007810 {is_inferred="true"} ! anatomical perturbation phenotypic evidence used in automatic assertion +intersection_of: ECO:0001137 ! xenotransplantation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007461 +name: WST-1 assay evidence used in automatic assertion +def: "A type of WST-1 assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001136 {is_inferred="true"} ! WST-1 assay evidence +is_a: ECO:0007775 {is_inferred="true"} ! cell proliferation assay evidence used in automatic assertion +intersection_of: ECO:0001136 ! WST-1 assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007462 +name: urine test evidence used in automatic assertion +def: "A type of urine test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001134 {is_inferred="true"} ! urine test evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001134 ! urine test evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007463 +name: terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in automatic assertion +def: "A type of terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001133 {is_inferred="true"} ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence +is_a: ECO:0007777 {is_inferred="true"} ! apoptotic assay evidence used in automatic assertion +intersection_of: ECO:0001133 ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007464 +name: tryptic phosphopeptide mapping assay evidence used in automatic assertion +def: "A type of tryptic phosphopeptide mapping assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001132 {is_inferred="true"} ! tryptic phosphopeptide mapping assay evidence +is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion +intersection_of: ECO:0001132 ! tryptic phosphopeptide mapping assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007465 +name: transgenic organism evidence used in automatic assertion +def: "A type of transgenic organism evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001131 {is_inferred="true"} ! transgenic organism evidence +is_a: ECO:0007427 {is_inferred="true"} ! genetic transformation evidence used in automatic assertion +intersection_of: ECO:0001131 ! transgenic organism evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007466 +name: tissue microarray evidence used in automatic assertion +def: "A type of tissue microarray evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001130 {is_inferred="true"} ! tissue microarray evidence +is_a: ECO:0007375 {is_inferred="true"} ! cytochemistry evidence used in automatic assertion +intersection_of: ECO:0001130 ! tissue microarray evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007467 +name: TACE activity assay evidence used in automatic assertion +def: "A type of TACE activity assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001129 {is_inferred="true"} ! TACE activity assay evidence +is_a: ECO:0007468 {is_inferred="true"} ! enzymatic activity assay evidence used in automatic assertion +intersection_of: ECO:0001129 ! TACE activity assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007468 +name: enzymatic activity assay evidence used in automatic assertion +def: "A type of enzyme assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000005 {is_inferred="true"} ! enzymatic activity assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000005 ! enzymatic activity assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007469 +name: surface plasmon resonance evidence used in automatic assertion +def: "A type of surface plasmon resonance evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001127 {is_inferred="true"} ! surface plasmon resonance evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +intersection_of: ECO:0001127 ! surface plasmon resonance evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007470 +name: restriction landmark genomic scanning evidence used in automatic assertion +def: "A type of restriction landmark genomic scanning evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000089 {is_inferred="true"} ! restriction landmark genomic scanning evidence +is_a: ECO:0007817 {is_inferred="true"} ! DNA detection assay evidence used in automatic assertion +intersection_of: ECO:0000089 ! restriction landmark genomic scanning evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007471 +name: resonant mirror biosensor evidence used in automatic assertion +def: "A type of resonant mirror biosensor evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001123 {is_inferred="true"} ! resonant mirror biosensor evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001123 ! resonant mirror biosensor evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007472 +name: high-performance liquid chromatography evidence used in automatic assertion +def: "A type of high-performance liquid chromatography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001052 {is_inferred="true"} ! high-performance liquid chromatography evidence +is_a: ECO:0007681 {is_inferred="true"} ! chromatography evidence used in automatic assertion +intersection_of: ECO:0001052 ! high-performance liquid chromatography evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007473 +name: ectopic expression evidence used in automatic assertion +def: "A type of ectopic expression evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000017 {is_inferred="true"} ! ectopic expression evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0000017 ! ectopic expression evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007474 +name: electrophoretic mobility shift assay evidence used in automatic assertion +def: "A type of electrophoretic mobility shift assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000096 {is_inferred="true"} ! electrophoretic mobility shift assay evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +intersection_of: ECO:0000096 ! electrophoretic mobility shift assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007475 +name: reverse transcription polymerase chain reaction evidence used in automatic assertion +alt_id: ECO:000108 +def: "A type of reverse transcription polymerase chain reaction evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000109 {is_inferred="true"} ! reverse transcription polymerase chain reaction evidence +is_a: ECO:0007818 {is_inferred="true"} ! RNA detection assay evidence used in automatic assertion +intersection_of: ECO:0000109 ! reverse transcription polymerase chain reaction evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007476 +name: in vivo polyADP-ribosylation assay evidence used in automatic assertion +def: "A type of in vivo polyADP-ribosylation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005500 {is_inferred="true"} ! in vivo polyADP-ribosylation assay evidence +is_a: ECO:0007797 {is_inferred="true"} ! polyADP-ribosylation assay evidence used in automatic assertion +intersection_of: ECO:0005500 ! in vivo polyADP-ribosylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007477 +name: DNA dot blot assay evidence used in automatic assertion +def: "A type of DNA dot blot assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005522 {is_inferred="true"} ! DNA dot blot assay evidence +is_a: ECO:0007817 {is_inferred="true"} ! DNA detection assay evidence used in automatic assertion +intersection_of: ECO:0005522 ! DNA dot blot assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007478 +name: random mutagenesis evidence used in automatic assertion +def: "A type of random mutagenesis phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005529 {is_inferred="true"} ! random mutagenesis phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0005529 ! random mutagenesis phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007479 +name: biological system reconstruction evidence by experimental evidence from single species used in automatic assertion +def: "A type of biological system reconstruction evidence by experimental evidence from single species that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005553 {is_inferred="true"} ! biological system reconstruction evidence by experimental evidence from single species +is_a: ECO:0007828 {is_inferred="true"} ! biological system reconstruction evidence by experimental evidence used in automatic assertion +intersection_of: ECO:0005553 ! biological system reconstruction evidence by experimental evidence from single species +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007480 +name: biological system reconstruction evidence by experimental evidence from mixed species used in automatic assertion +def: "A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005552 {is_inferred="true"} ! biological system reconstruction evidence by experimental evidence from mixed species +is_a: ECO:0007828 {is_inferred="true"} ! biological system reconstruction evidence by experimental evidence used in automatic assertion +intersection_of: ECO:0005552 ! biological system reconstruction evidence by experimental evidence from mixed species +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007481 +name: biological system reconstruction evidence based on orthology evidence used in automatic assertion +def: "A type of biological system reconstruction evidence based on orthology evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005545 {is_inferred="true"} ! biological system reconstruction evidence based on orthology evidence +is_a: ECO:0007482 {is_inferred="true"} ! biological system reconstruction evidence based on homology evidence used in automatic assertion +intersection_of: ECO:0005545 ! biological system reconstruction evidence based on orthology evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007482 +name: biological system reconstruction evidence based on homology evidence used in automatic assertion +def: "A type of biological system reconstruction evidence based on homology evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005549 {is_inferred="true"} ! biological system reconstruction evidence based on homology evidence +is_a: ECO:0007827 {is_inferred="true"} ! biological system reconstruction evidence used in automatic assertion +intersection_of: ECO:0005549 ! biological system reconstruction evidence based on homology evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007483 +name: biological system reconstruction evidence based on paralogy evidence used in automatic assertion +def: "A type of biological system reconstruction evidence based on paralogy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005550 {is_inferred="true"} ! biological system reconstruction evidence based on paralogy evidence +is_a: ECO:0007482 {is_inferred="true"} ! biological system reconstruction evidence based on homology evidence used in automatic assertion +intersection_of: ECO:0005550 ! biological system reconstruction evidence based on paralogy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007484 +name: biological system reconstruction evidence based on inference from background scientific knowledge used in automatic assertion +def: "A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005548 {is_inferred="true"} ! biological system reconstruction evidence based on inference from background scientific knowledge +is_a: ECO:0007827 {is_inferred="true"} ! biological system reconstruction evidence used in automatic assertion +intersection_of: ECO:0005548 ! biological system reconstruction evidence based on inference from background scientific knowledge +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007485 +name: immunogold labelling evidence used in automatic assertion +def: "A type of immunogold labelling evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000090 {is_inferred="true"} ! immunogold labelling evidence +is_a: ECO:0007486 {is_inferred="true"} ! immunolocalization evidence used in automatic assertion +intersection_of: ECO:0000090 ! immunogold labelling evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007486 +name: immunolocalization evidence used in automatic assertion +def: "A type of immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000087 {is_inferred="true"} ! immunolocalization evidence +is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion +is_a: ECO:0007796 {is_inferred="true"} ! immunodetection assay evidence used in automatic assertion +intersection_of: ECO:0000087 ! immunolocalization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007487 +name: flow cytometry evidence used in automatic assertion +def: "A type of flow cytometry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000268 {is_inferred="true"} ! flow cytometry evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000268 ! flow cytometry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007488 +name: enzyme-linked immunoabsorbent assay evidence used in automatic assertion +def: "A type of enzyme-linked immunoabsorbent assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000267 {is_inferred="true"} ! enzyme-linked immunoabsorbent assay evidence +is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion +intersection_of: ECO:0000267 ! enzyme-linked immunoabsorbent assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007489 +name: high throughput mass spectrometry evidence used in automatic assertion +def: "A type of high throughput mass spectrometry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005585 {is_inferred="true"} ! high throughput mass spectrometry evidence +is_a: ECO:0007433 {is_inferred="true"} ! mass spectrometry evidence used in automatic assertion +intersection_of: ECO:0005585 ! high throughput mass spectrometry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007490 +name: confocal microscopy evidence used in automatic assertion +def: "A type of confocal microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005587 {is_inferred="true"} ! confocal microscopy evidence +is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion +intersection_of: ECO:0005587 ! confocal microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007491 +name: wide-field microscopy evidence used in automatic assertion +def: "A type of wide-field microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005588 {is_inferred="true"} ! wide-field microscopy evidence +is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion +intersection_of: ECO:0005588 ! wide-field microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007492 +name: immunogold labelling electron microscopy assay evidence used in automatic assertion +def: "A type of immunogold labelling electron microscopy assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005591 {is_inferred="true"} ! immunogold labelling electron microscopy assay evidence +is_a: ECO:0007485 {is_inferred="true"} ! immunogold labelling evidence used in automatic assertion +is_a: ECO:0007493 {is_inferred="true"} ! electron microscopy evidence used in automatic assertion +intersection_of: ECO:0005591 ! immunogold labelling electron microscopy assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007493 +name: electron microscopy evidence used in automatic assertion +def: "A type of electron microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005033 {is_inferred="true"} ! electron microscopy evidence +is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion +is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion +intersection_of: ECO:0005033 ! electron microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007494 +name: immunoperoxidase immunolocalization evidence used in automatic assertion +def: "A type of immunoperoxidase immunolocalization evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005594 {is_inferred="true"} ! immunoperoxidase immunolocalization evidence +is_a: ECO:0007486 {is_inferred="true"} ! immunolocalization evidence used in automatic assertion +intersection_of: ECO:0005594 ! immunoperoxidase immunolocalization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007495 +name: immunoperoxidase immunolocalization electron microscopy evidence used in automatic assertion +def: "A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005596 {is_inferred="true"} ! immunoperoxidase immunolocalization electron microscopy evidence +is_a: ECO:0007493 {is_inferred="true"} ! electron microscopy evidence used in automatic assertion +is_a: ECO:0007494 {is_inferred="true"} ! immunoperoxidase immunolocalization evidence used in automatic assertion +intersection_of: ECO:0005596 ! immunoperoxidase immunolocalization electron microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007496 +name: immunofluorescence confocal microscopy evidence used in automatic assertion +def: "A type of immunofluorescence confocal microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005600 {is_inferred="true"} ! immunofluorescence confocal microscopy evidence +is_a: ECO:0007490 {is_inferred="true"} ! confocal microscopy evidence used in automatic assertion +is_a: ECO:0007497 {is_inferred="true"} ! immunofluorescence evidence used in automatic assertion +intersection_of: ECO:0005600 ! immunofluorescence confocal microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007497 +name: immunofluorescence evidence used in automatic assertion +def: "A type of immunofluorescence evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000007 {is_inferred="true"} ! immunofluorescence evidence +is_a: ECO:0007819 {is_inferred="true"} ! protein detection assay evidence used in automatic assertion +intersection_of: ECO:0000007 ! immunofluorescence evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007498 +name: two-dimensional polyacrylamide gel electrophoresis evidence used in automatic assertion +def: "A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005503 {is_inferred="true"} ! two-dimensional polyacrylamide gel electrophoresis evidence +is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion +intersection_of: ECO:0005503 ! two-dimensional polyacrylamide gel electrophoresis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007499 +name: alkaline phosphatase reporter gene assay evidence used in automatic assertion +def: "A type of alkaline phosphatase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001801 {is_inferred="true"} ! alkaline phosphatase reporter gene assay evidence +is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion +intersection_of: ECO:0001801 ! alkaline phosphatase reporter gene assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007500 +name: beta-galactosidase reporter gene assay evidence used in automatic assertion +def: "A type of beta-galactosidase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "LacZ transcript localization evidence used in automatic assertion" EXACT [] +is_a: ECO:0001802 {is_inferred="true"} ! beta-galactosidase reporter gene assay evidence +is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion +intersection_of: ECO:0001802 ! beta-galactosidase reporter gene assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007501 +name: chloramphenicol acetyltransferase reporter gene assay evidence used in automatic assertion +def: "A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001803 {is_inferred="true"} ! chloramphenicol acetyltransferase reporter gene assay evidence +is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion +intersection_of: ECO:0001803 ! chloramphenicol acetyltransferase reporter gene assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007502 +name: chromatin immunoprecipitation-PCR evidence used in automatic assertion +def: "A type of chromatin immunoprecipitation-PCR evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000227 {is_inferred="true"} ! chromatin immunoprecipitation-PCR evidence +is_a: ECO:0007786 {is_inferred="true"} ! chromatin immunoprecipitation evidence used in automatic assertion +intersection_of: ECO:0000227 ! chromatin immunoprecipitation-PCR evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007503 +name: immunoprecipitation evidence used in automatic assertion +def: "A type of immunoprecipitation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000085 {is_inferred="true"} ! immunoprecipitation evidence +is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion +intersection_of: ECO:0000085 ! immunoprecipitation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007504 +name: copper-phenanthroline footprinting evidence used in automatic assertion +def: "A type of copper-phenanthroline footprinting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001815 {is_inferred="true"} ! copper-phenanthroline footprinting evidence +is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion +intersection_of: ECO:0001815 ! copper-phenanthroline footprinting evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007505 +name: nucleic acid binding evidence used in automatic assertion +def: "A type of nucleic acid binding evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000136 {is_inferred="true"} ! nucleic acid binding evidence +is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion +intersection_of: ECO:0000136 ! nucleic acid binding evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007506 +name: DNA affinity chromatography evidence used in automatic assertion +def: "A type of DNA affinity chromatography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001809 {is_inferred="true"} ! DNA affinity chromatography evidence +is_a: ECO:0007681 {is_inferred="true"} ! chromatography evidence used in automatic assertion +intersection_of: ECO:0001809 ! DNA affinity chromatography evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007507 +name: DNAse footprinting evidence used in automatic assertion +def: "A type of DNAse footprinting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001810 {is_inferred="true"} ! DNAse footprinting evidence +is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion +intersection_of: ECO:0001810 ! DNAse footprinting evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007508 +name: fluorescence anisotropy evidence used in automatic assertion +def: "A type of fluorescence anisotropy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001811 {is_inferred="true"} ! fluorescence anisotropy evidence +is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion +intersection_of: ECO:0001811 ! fluorescence anisotropy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007509 +name: ferric uptake regulator titration assay evidence used in automatic assertion +def: "A type of ferric uptake regulator titration assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001829 {is_inferred="true"} ! ferric uptake regulator titration assay evidence +is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion +intersection_of: ECO:0001829 ! ferric uptake regulator titration assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007510 +name: systematic evolution of ligands by exponential amplification evidence used in automatic assertion +def: "A type of systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000293 {is_inferred="true"} ! systematic evolution of ligands by exponential amplification evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +intersection_of: ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007511 +name: glutathione S-transferase pull-down assay evidence used in automatic assertion +def: "A type of glutathione S-transferase pull-down assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001817 {is_inferred="true"} ! glutathione S-transferase pull-down assay evidence +is_a: ECO:0007432 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in automatic assertion +intersection_of: ECO:0001817 ! glutathione S-transferase pull-down assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007512 +name: beta-glucuronidase reporter gene assay evidence used in automatic assertion +def: "A type of beta-glucuronidase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001804 {is_inferred="true"} ! beta-glucuronidase reporter gene assay evidence +is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion +intersection_of: ECO:0001804 ! beta-glucuronidase reporter gene assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007513 +name: heteronuclear single quantum coherence spectroscopy evidence used in automatic assertion +def: "A type of heteronuclear single quantum coherence spectroscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001813 {is_inferred="true"} ! heteronuclear single quantum coherence spectroscopy evidence +is_a: ECO:0006164 {is_inferred="true"} ! nuclear magnetic resonance spectroscopy evidence used in automatic assertion +intersection_of: ECO:0001813 ! heteronuclear single quantum coherence spectroscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007514 +name: hydroxyl-radical footprinting evidence used in automatic assertion +def: "A type of hydroxyl-radical footprinting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001818 {is_inferred="true"} ! hydroxyl-radical footprinting evidence +is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion +intersection_of: ECO:0001818 ! hydroxyl-radical footprinting evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007515 +name: isothermal titration calorimetry evidence used in automatic assertion +def: "A type of isothermal titration calorimetry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001825 {is_inferred="true"} ! isothermal titration calorimetry evidence +is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion +intersection_of: ECO:0001825 ! isothermal titration calorimetry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007516 +name: luciferase reporter gene assay evidence used in automatic assertion +def: "A type of luciferase reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001805 {is_inferred="true"} ! luciferase reporter gene assay evidence +is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion +intersection_of: ECO:0001805 ! luciferase reporter gene assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007517 +name: methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in automatic assertion +def: "A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001814 {is_inferred="true"} ! methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence +is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion +intersection_of: ECO:0001814 ! methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007518 +name: northern blot evidence used in automatic assertion +def: "A type of northern blot evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000106 {is_inferred="true"} ! northern blot evidence +is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion +intersection_of: ECO:0000106 ! northern blot evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007519 +name: methylation interference footprinting evidence used in automatic assertion +def: "A type of methylation interference footprinting evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "premethylation interference footprinting evidence used in automatic assertion" EXACT [] +is_a: ECO:0001827 {is_inferred="true"} ! methylation interference footprinting evidence +is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion +intersection_of: ECO:0001827 ! methylation interference footprinting evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007520 +name: primer extension assay evidence used in automatic assertion +def: "A type of primer extension assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001819 {is_inferred="true"} ! primer extension assay evidence +is_a: ECO:0007303 {is_inferred="true"} ! transcript expression evidence used in automatic assertion +intersection_of: ECO:0001819 ! primer extension assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007521 +name: quantitative polymerase chain reaction evidence used in automatic assertion +def: "A type of quantitative polymerase chain reaction evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000231 {is_inferred="true"} ! quantitative polymerase chain reaction evidence +is_a: ECO:0007817 {is_inferred="true"} ! DNA detection assay evidence used in automatic assertion +intersection_of: ECO:0000231 ! quantitative polymerase chain reaction evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007522 +name: rapid amplification of cDNA ends polymerase chain reaction evidence used in automatic assertion +def: "A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001820 {is_inferred="true"} ! rapid amplification of cDNA ends polymerase chain reaction evidence +is_a: ECO:0007813 {is_inferred="true"} ! nucleotide sequencing assay evidence used in automatic assertion +intersection_of: ECO:0001820 ! rapid amplification of cDNA ends polymerase chain reaction evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007523 +name: S1 nuclease protection assay evidence used in automatic assertion +def: "A type of S1 nuclease protection assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005521 {is_inferred="true"} ! S1 nuclease protection assay evidence +is_a: ECO:0007316 {is_inferred="true"} ! nuclease protection assay evidence used in automatic assertion +intersection_of: ECO:0005521 ! S1 nuclease protection assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007524 +name: site-directed mutagenesis phenotypic evidence used in automatic assertion +def: "A type of site-directed mutagenesis phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "site-directed mutagenesis evidence used in automatic assertion" EXACT [] +is_a: ECO:0005528 {is_inferred="true"} ! site-directed mutagenesis phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0005528 ! site-directed mutagenesis phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007525 +name: survival rate analysis evidence used in automatic assertion +def: "A type of survival rate analysis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001822 {is_inferred="true"} ! survival rate analysis evidence +is_a: ECO:0007370 {is_inferred="true"} ! knockout evidence used in automatic assertion +intersection_of: ECO:0001822 ! survival rate analysis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007526 +name: ultraviolet light footprinting evidence used in automatic assertion +def: "A type of ultraviolet light footprinting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001826 {is_inferred="true"} ! ultraviolet light footprinting evidence +is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion +intersection_of: ECO:0001826 ! ultraviolet light footprinting evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007527 +name: xylE reporter gene assay evidence used in automatic assertion +def: "A type of xylE reporter gene assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005537 {is_inferred="true"} ! xylE reporter gene assay evidence +is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion +intersection_of: ECO:0005537 ! xylE reporter gene assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007528 +name: ad-hoc qualitative phenotype observation evidence used in automatic assertion +def: "A type of ad-hoc qualitative phenotype observation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005673 {is_inferred="true"} ! ad-hoc qualitative phenotype observation evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0005673 ! ad-hoc qualitative phenotype observation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007529 +name: ad-hoc quantitative phenotype observation evidence used in automatic assertion +def: "A type of ad-hoc quantitative phenotype observation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005675 {is_inferred="true"} ! ad-hoc quantitative phenotype observation evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0005675 ! ad-hoc quantitative phenotype observation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007530 +name: cell transfection experiment evidence used in automatic assertion +def: "A type of cell transfection experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005606 {is_inferred="true"} ! cell transfection experiment evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0005606 ! cell transfection experiment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007531 +name: yeast 2-hybrid evidence used in automatic assertion +def: "A type of yeast 2-hybrid evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000068 {is_inferred="true"} ! yeast 2-hybrid evidence +is_a: ECO:0007432 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in automatic assertion +intersection_of: ECO:0000068 ! yeast 2-hybrid evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007532 +name: Cya fusion reporter assay evidence used in automatic assertion +def: "A type of Cya fusion reporter assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006001 {is_inferred="true"} ! Cya fusion reporter assay evidence +is_a: ECO:0007473 {is_inferred="true"} ! ectopic expression evidence used in automatic assertion +is_a: ECO:0007765 {is_inferred="true"} ! reporter gene assay evidence used in automatic assertion +intersection_of: ECO:0006001 ! Cya fusion reporter assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007533 +name: super-resolution microscopy evidence used in automatic assertion +def: "A type of super-resolution microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005578 {is_inferred="true"} ! super-resolution microscopy evidence +is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion +intersection_of: ECO:0005578 ! super-resolution microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007534 +name: fractionation evidence used in automatic assertion +def: "A type of fractionation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000100 {is_inferred="true"} ! fractionation evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000100 ! fractionation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007535 +name: electrophysiology assay evidence used in automatic assertion +def: "A type of electrophysiology assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000164 {is_inferred="true"} ! electrophysiology assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000164 ! electrophysiology assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007536 +name: mRNA interactome capture evidence used in automatic assertion +def: "A type of mRNA interactome capture evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006010 {is_inferred="true"} ! mRNA interactome capture evidence +is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion +intersection_of: ECO:0006010 ! mRNA interactome capture evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007537 +name: patch-clamp recording evidence used in automatic assertion +def: "A type of patch-clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006012 {is_inferred="true"} ! patch-clamp recording evidence +is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion +intersection_of: ECO:0006012 ! patch-clamp recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007538 +name: whole-cell patch-clamp recording evidence used in automatic assertion +def: "A type of whole-cell patch-clamp recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006014 {is_inferred="true"} ! whole-cell patch-clamp recording evidence +is_a: ECO:0007537 {is_inferred="true"} ! patch-clamp recording evidence used in automatic assertion +intersection_of: ECO:0006014 ! whole-cell patch-clamp recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007539 +name: author statement from published clinical study used in automatic assertion +def: "A type of author statement from published clinical study that is used in an automatic assertion." [ECO:RCT] +synonym: "traceable author statement from published clinical study used in automatic assertion" RELATED [] +is_a: ECO:0006016 {is_inferred="true"} ! author statement from published clinical study +is_a: ECO:0007321 {is_inferred="true"} ! author statement supported by traceable reference used in automatic assertion +intersection_of: ECO:0006016 ! author statement from published clinical study +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007540 +name: inference based on individual clinical experience used in automatic assertion +def: "A type of inference based on individual clinical experience that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006018 {is_inferred="true"} ! inference based on individual clinical experience +is_a: ECO:0007322 {is_inferred="true"} ! curator inference used in automatic assertion +intersection_of: ECO:0006018 ! inference based on individual clinical experience +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007541 +name: biofilm formation assay evidence used in automatic assertion +def: "A type of biofilm formation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006020 {is_inferred="true"} ! biofilm formation assay evidence +is_a: ECO:0007775 {is_inferred="true"} ! cell proliferation assay evidence used in automatic assertion +intersection_of: ECO:0006020 ! biofilm formation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007542 +name: microtiter plate biofilm assay evidence used in automatic assertion +def: "A type of microtiter plate biofilm assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006022 {is_inferred="true"} ! microtiter plate biofilm assay evidence +is_a: ECO:0007541 {is_inferred="true"} ! biofilm formation assay evidence used in automatic assertion +intersection_of: ECO:0006022 ! microtiter plate biofilm assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007543 +name: air-liquid interface assay evidence used in automatic assertion +def: "A type of air-liquid interface assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006024 {is_inferred="true"} ! air-liquid interface assay evidence +is_a: ECO:0007541 {is_inferred="true"} ! biofilm formation assay evidence used in automatic assertion +intersection_of: ECO:0006024 ! air-liquid interface assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007544 +name: colony biofilm assay evidence used in automatic assertion +def: "A type of colony biofilm assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006026 {is_inferred="true"} ! colony biofilm assay evidence +is_a: ECO:0007541 {is_inferred="true"} ! biofilm formation assay evidence used in automatic assertion +intersection_of: ECO:0006026 ! colony biofilm assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007545 +name: Kadouri drip-fed biofilm assay evidence used in automatic assertion +def: "A type of Kadouri drip-fed biofilm assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006028 {is_inferred="true"} ! Kadouri drip-fed biofilm assay evidence +is_a: ECO:0007541 {is_inferred="true"} ! biofilm formation assay evidence used in automatic assertion +intersection_of: ECO:0006028 ! Kadouri drip-fed biofilm assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007546 +name: co-immunoprecipitation evidence used in automatic assertion +def: "A type of co-immunoprecipitation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000070 {is_inferred="true"} ! co-immunoprecipitation evidence +is_a: ECO:0007503 {is_inferred="true"} ! immunoprecipitation evidence used in automatic assertion +intersection_of: ECO:0000070 ! co-immunoprecipitation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007547 +name: optogenetic evidence used in automatic assertion +def: "A type of optogenetic evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006032 {is_inferred="true"} ! optogenetic evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0006032 ! optogenetic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007548 +name: fluorescent sensor evidence used in automatic assertion +def: "A type of fluorescent sensor evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006034 {is_inferred="true"} ! fluorescent sensor evidence +is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion +intersection_of: ECO:0006034 ! fluorescent sensor evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007549 +name: genetically encoded fluorescent sensor evidence used in automatic assertion +def: "A type of genetically encoded fluorescent sensor evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006036 {is_inferred="true"} ! genetically encoded fluorescent sensor evidence +is_a: ECO:0007548 {is_inferred="true"} ! fluorescent sensor evidence used in automatic assertion +intersection_of: ECO:0006036 ! genetically encoded fluorescent sensor evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007550 +name: genetically encoded fluorescent electrophysiology assay evidence used in automatic assertion +def: "A type of genetically encoded fluorescent electrophysiology assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006038 {is_inferred="true"} ! genetically encoded fluorescent electrophysiology assay evidence +is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion +is_a: ECO:0007548 {is_inferred="true"} ! fluorescent sensor evidence used in automatic assertion +intersection_of: ECO:0006038 ! genetically encoded fluorescent electrophysiology assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007551 +name: genetically encoded fluorescent ion concentration sensor assay evidence used in automatic assertion +def: "A type of genetically encoded fluorescent ion concentration sensor assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006040 {is_inferred="true"} ! genetically encoded fluorescent ion concentration sensor assay evidence +is_a: ECO:0007550 {is_inferred="true"} ! genetically encoded fluorescent electrophysiology assay evidence used in automatic assertion +intersection_of: ECO:0006040 ! genetically encoded fluorescent ion concentration sensor assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007552 +name: cell fractionation evidence used in automatic assertion +def: "A type of cell fractionation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000004 {is_inferred="true"} ! cell fractionation evidence +is_a: ECO:0007534 {is_inferred="true"} ! fractionation evidence used in automatic assertion +intersection_of: ECO:0000004 ! cell fractionation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007553 +name: extracellular recording evidence used in automatic assertion +def: "A type of extracellular recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006043 {is_inferred="true"} ! extracellular recording evidence +is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion +intersection_of: ECO:0006043 ! extracellular recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007554 +name: single-unit extracellular recording evidence used in automatic assertion +def: "A type of single-unit extracellular recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006045 {is_inferred="true"} ! single-unit extracellular recording evidence +is_a: ECO:0007553 {is_inferred="true"} ! extracellular recording evidence used in automatic assertion +intersection_of: ECO:0006045 ! single-unit extracellular recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007555 +name: field potential recording evidence used in automatic assertion +def: "A type of field potential recording evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006047 {is_inferred="true"} ! field potential recording evidence +is_a: ECO:0007553 {is_inferred="true"} ! extracellular recording evidence used in automatic assertion +intersection_of: ECO:0006047 ! field potential recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007556 +name: anti-sense experiment evidence used in automatic assertion +def: "A type of anti-sense experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000018 {is_inferred="true"} ! anti-sense experiment evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0000018 ! anti-sense experiment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007557 +name: morpholino experiment evidence used in automatic assertion +def: "A type of morpholino experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000292 {is_inferred="true"} ! morpholino experiment evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0000292 ! morpholino experiment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007558 +name: RNAi evidence used in automatic assertion +def: "A type of RNAi evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000019 {is_inferred="true"} ! RNAi evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0000019 ! RNAi evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007559 +name: pharmacological assay evidence used in automatic assertion +def: "A type of pharmacological assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006053 {is_inferred="true"} ! pharmacological assay evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0006053 ! pharmacological assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007560 +name: immunofluorescence wide-field microscopy evidence used in automatic assertion +def: "A type of immunofluorescence wide-field microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005599 {is_inferred="true"} ! immunofluorescence wide-field microscopy evidence +is_a: ECO:0007497 {is_inferred="true"} ! immunofluorescence evidence used in automatic assertion +is_a: ECO:0007561 {is_inferred="true"} ! wide-field fluorescence microscopy evidence used in automatic assertion +intersection_of: ECO:0005599 ! immunofluorescence wide-field microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007561 +name: wide-field fluorescence microscopy evidence used in automatic assertion +def: "A type of wide-field fluorescence microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0005598 {is_inferred="true"} ! wide-field fluorescence microscopy evidence +is_a: ECO:0007491 {is_inferred="true"} ! wide-field microscopy evidence used in automatic assertion +intersection_of: ECO:0005598 ! wide-field fluorescence microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007562 +name: over expression analysis evidence used in automatic assertion +def: "A type of over expression analysis evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000120 {is_inferred="true"} ! over expression analysis evidence +is_a: ECO:0007427 {is_inferred="true"} ! genetic transformation evidence used in automatic assertion +intersection_of: ECO:0000120 ! over expression analysis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007563 +name: cell-free assay evidence used in automatic assertion +def: "A type of cell-free assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000183 {is_inferred="true"} ! cell-free assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000183 ! cell-free assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007564 +name: fluorescence recovery after photobleaching evidence used in automatic assertion +def: "A type of fluorescence recovery after photobleaching evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006066 {is_inferred="true"} ! fluorescence recovery after photobleaching evidence +is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion +intersection_of: ECO:0006066 ! fluorescence recovery after photobleaching evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007565 +name: immuno-labelling electron microscopy evidence used in automatic assertion +def: "A type of immuno-labelling electron microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006070 {is_inferred="true"} ! immuno-labelling electron microscopy evidence +is_a: ECO:0007493 {is_inferred="true"} ! electron microscopy evidence used in automatic assertion +intersection_of: ECO:0006070 ! immuno-labelling electron microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007566 +name: immunofluorescence super resolution microscopy evidence used in automatic assertion +def: "A type of immunofluorescence super resolution microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006072 {is_inferred="true"} ! immunofluorescence super resolution microscopy evidence +is_a: ECO:0007533 {is_inferred="true"} ! super-resolution microscopy evidence used in automatic assertion +intersection_of: ECO:0006072 ! immunofluorescence super resolution microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007567 +name: co-purification evidence used in automatic assertion +def: "A type of co-purification evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000022 {is_inferred="true"} ! co-purification evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +intersection_of: ECO:0000022 ! co-purification evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007568 +name: yeast one-hybrid evidence used in automatic assertion +def: "A type of yeast one-hybrid evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000066 {is_inferred="true"} ! yeast one-hybrid evidence +is_a: ECO:0007432 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in automatic assertion +intersection_of: ECO:0000066 ! yeast one-hybrid evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007569 +name: split-ubiquitin functional complementation evidence used in automatic assertion +def: "A type of split-ubiquitin functional complementation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000074 {is_inferred="true"} ! split-ubiquitin functional complementation evidence +is_a: ECO:0006294 {is_inferred="true"} ! protein fragment functional complementation evidence used in automatic assertion +intersection_of: ECO:0000074 ! split-ubiquitin functional complementation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007570 +name: far-Western blotting evidence used in automatic assertion +def: "A type of far-Western blotting evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000076 {is_inferred="true"} ! far-Western blotting evidence +is_a: ECO:0007332 {is_inferred="true"} ! physical interaction evidence used in automatic assertion +intersection_of: ECO:0000076 ! far-Western blotting evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007571 +name: affinity chromatography evidence used in automatic assertion +def: "A type of affinity chromatography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000079 {is_inferred="true"} ! affinity chromatography evidence +is_a: ECO:0007384 {is_inferred="true"} ! affinity evidence used in automatic assertion +intersection_of: ECO:0000079 ! affinity chromatography evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007572 +name: ribohomopolymer binding assay evidence used in automatic assertion +def: "A type of ribohomopolymer binding assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000138 {is_inferred="true"} ! ribohomopolymer binding assay evidence +is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion +intersection_of: ECO:0000138 ! ribohomopolymer binding assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007573 +name: protein:ion binding evidence used in automatic assertion +def: "A type of protein:ion binding evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000142 {is_inferred="true"} ! protein:ion binding evidence +is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion +intersection_of: ECO:0000142 ! protein:ion binding evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007574 +name: Southwestern blot evidence used in automatic assertion +def: "A type of Southwestern blot evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000144 {is_inferred="true"} ! Southwestern blot evidence +is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion +intersection_of: ECO:0000144 ! Southwestern blot evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007575 +name: Northwestern blot evidence used in automatic assertion +def: "A type of Northwestern blot evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000146 {is_inferred="true"} ! Northwestern blot evidence +is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion +intersection_of: ECO:0000146 ! Northwestern blot evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007576 +name: bacterial one-hybrid evidence used in automatic assertion +def: "A type of bacterial one-hybrid evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000294 {is_inferred="true"} ! bacterial one-hybrid evidence +is_a: ECO:0007432 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in automatic assertion +intersection_of: ECO:0000294 ! bacterial one-hybrid evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007577 +name: protein-oligonucleotide microarray binding evidence used in automatic assertion +def: "A type of protein-oligonucleotide microarray binding evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0001601 {is_inferred="true"} ! protein-oligonucleotide microarray binding evidence +is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion +is_a: ECO:0007505 {is_inferred="true"} ! nucleic acid binding evidence used in automatic assertion +intersection_of: ECO:0001601 ! protein-oligonucleotide microarray binding evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007578 +name: functional complementation evidence used in automatic assertion +def: "A type of functional complementation evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000012 {is_inferred="true"} ! functional complementation evidence +is_a: ECO:0007326 {is_inferred="true"} ! genetic interaction evidence used in automatic assertion +intersection_of: ECO:0000012 ! functional complementation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007579 +name: transgenic rescue experiment evidence used in automatic assertion +def: "A type of transgenic rescue experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000013 {is_inferred="true"} ! transgenic rescue experiment evidence +is_a: ECO:0007578 {is_inferred="true"} ! functional complementation evidence used in automatic assertion +intersection_of: ECO:0000013 ! transgenic rescue experiment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007580 +name: transient rescue experiment evidence used in automatic assertion +def: "A type of transient rescue experiment evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000038 {is_inferred="true"} ! transient rescue experiment evidence +is_a: ECO:0007578 {is_inferred="true"} ! functional complementation evidence used in automatic assertion +intersection_of: ECO:0000038 ! transient rescue experiment evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007581 +name: suppressor/enhancer interaction phenotypic evidence used in automatic assertion +def: "A type of suppressor/enhancer interaction phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "suppressor/enhancer interaction evidence used in automatic assertion" EXACT [] +is_a: ECO:0000052 {is_inferred="true"} ! suppressor/enhancer interaction phenotypic evidence +is_a: ECO:0007326 {is_inferred="true"} ! genetic interaction evidence used in automatic assertion +intersection_of: ECO:0000052 ! suppressor/enhancer interaction phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007582 +name: double mutant phenotypic evidence used in automatic assertion +def: "A type of double mutant phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "double mutant phenotype evidence used in automatic assertion" EXACT [] +is_a: ECO:0000054 {is_inferred="true"} ! double mutant phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0000054 ! double mutant phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007583 +name: epistatic interaction phenotypic evidence used in automatic assertion +def: "A type of epistatic interaction phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "epistatic interaction evidence used in automatic assertion" EXACT [] +is_a: ECO:0000056 {is_inferred="true"} ! epistatic interaction phenotypic evidence +is_a: ECO:0007326 {is_inferred="true"} ! genetic interaction evidence used in automatic assertion +intersection_of: ECO:0000056 ! epistatic interaction phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007584 +name: functional complementation in heterologous system evidence used in automatic assertion +def: "A type of functional complementation in heterologous system evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000064 {is_inferred="true"} ! functional complementation in heterologous system evidence +is_a: ECO:0007578 {is_inferred="true"} ! functional complementation evidence used in automatic assertion +intersection_of: ECO:0000064 ! functional complementation in heterologous system evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007585 +name: temperature-sensitive mutant phenotypic evidence used in automatic assertion +def: "A type of temperature-sensitive mutant phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "temperature-sensitive mutant phenotype evidence used in automatic assertion" EXACT [] +is_a: ECO:0006098 {is_inferred="true"} ! temperature-sensitive mutant phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0006098 ! temperature-sensitive mutant phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007586 +name: recessive mutant phenotype evidence used in automatic assertion +def: "A type of recessive mutant phenotype evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006100 {is_inferred="true"} ! recessive mutant phenotype evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0006100 ! recessive mutant phenotype evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007587 +name: high throughput mutant phenotypic evidence used in automatic assertion +def: "A type of high throughput mutant phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "high throughput mutant phenotype evidence used in automatic assertion" EXACT [] +is_a: ECO:0006057 {is_inferred="true"} ! high throughput evidence used in automatic assertion +is_a: ECO:0007000 {is_inferred="true"} ! high throughput mutant phenotypic evidence +is_a: ECO:0007325 {is_inferred="true"} ! mutant phenotype evidence used in automatic assertion +intersection_of: ECO:0007000 ! high throughput mutant phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007588 +name: high throughput genetic interaction phenotypic evidence used in automatic assertion +def: "A type of high throughput genetic interaction phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "high throughput genetic interaction evidence used in automatic assertion" EXACT [] +is_a: ECO:0006057 {is_inferred="true"} ! high throughput evidence used in automatic assertion +is_a: ECO:0007002 {is_inferred="true"} ! high throughput genetic interaction phenotypic evidence +is_a: ECO:0007326 {is_inferred="true"} ! genetic interaction evidence used in automatic assertion +intersection_of: ECO:0007002 ! high throughput genetic interaction phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007589 +name: high throughput direct assay evidence used in automatic assertion +def: "A type of high throughput direct assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006057 {is_inferred="true"} ! high throughput evidence used in automatic assertion +is_a: ECO:0007004 {is_inferred="true"} ! high throughput direct assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0007004 ! high throughput direct assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007590 +name: high throughput expression pattern evidence used in automatic assertion +def: "A type of high throughput expression pattern evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006057 {is_inferred="true"} ! high throughput evidence used in automatic assertion +is_a: ECO:0007006 {is_inferred="true"} ! high throughput expression pattern evidence +is_a: ECO:0007298 {is_inferred="true"} ! expression pattern evidence used in automatic assertion +intersection_of: ECO:0007006 ! high throughput expression pattern evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007591 +name: radioligand binding assay evidence used in automatic assertion +def: "A type of radioligand binding assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007008 {is_inferred="true"} ! radioligand binding assay evidence +is_a: ECO:0007373 {is_inferred="true"} ! protein binding evidence used in automatic assertion +intersection_of: ECO:0007008 ! radioligand binding assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007592 +name: combinatorial experimental and author inference evidence used in automatic assertion +def: "A type of combinatorial evidence from author knowledge and experimental evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "combinatorial evidence from author knowledge and experimental evidence used in automatic assertion" EXACT [] +is_a: ECO:0000213 {is_inferred="true"} ! combinatorial evidence used in automatic assertion +is_a: ECO:0007011 {is_inferred="true"} ! combinatorial experimental and author inference evidence +intersection_of: ECO:0007011 ! combinatorial experimental and author inference evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007593 +name: combinatorial experimental and curator inference evidence used in automatic assertion +def: "A type of combinatorial evidence from curator knowledge and experimental evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "combinatorial evidence from curator knowledge and experimental evidence used in automatic assertion" EXACT [] +is_a: ECO:0000213 {is_inferred="true"} ! combinatorial evidence used in automatic assertion +is_a: ECO:0007012 {is_inferred="true"} ! combinatorial experimental and curator inference evidence +intersection_of: ECO:0007012 ! combinatorial experimental and curator inference evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007594 +name: voltammetry evidence used in automatic assertion +def: "A type of voltammetry evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007015 {is_inferred="true"} ! voltammetry evidence +is_a: ECO:0007805 {is_inferred="true"} ! substance quantification evidence used in automatic assertion +intersection_of: ECO:0007015 ! voltammetry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007595 +name: photoconversion evidence used in automatic assertion +def: "A type of photoconversion evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007017 {is_inferred="true"} ! photoconversion evidence +is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion +intersection_of: ECO:0007017 ! photoconversion evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007596 +name: agglutination test evidence used in automatic assertion +def: "A type of agglutination test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007019 {is_inferred="true"} ! agglutination test evidence +is_a: ECO:0007348 {is_inferred="true"} ! immunological assay evidence used in automatic assertion +intersection_of: ECO:0007019 ! agglutination test evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007597 +name: slide agglutination test evidence used in automatic assertion +def: "A type of slide agglutination test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007021 {is_inferred="true"} ! slide agglutination test evidence +is_a: ECO:0007596 {is_inferred="true"} ! agglutination test evidence used in automatic assertion +intersection_of: ECO:0007021 ! slide agglutination test evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007598 +name: direct Coombs test evidence used in automatic assertion +def: "A type of direct Coombs test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007023 {is_inferred="true"} ! direct Coombs test evidence +is_a: ECO:0007596 {is_inferred="true"} ! agglutination test evidence used in automatic assertion +intersection_of: ECO:0007023 ! direct Coombs test evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007599 +name: indirect Coombs test evidence used in automatic assertion +def: "A type of indirect Coombs test evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007025 {is_inferred="true"} ! indirect Coombs test evidence +is_a: ECO:0007596 {is_inferred="true"} ! agglutination test evidence used in automatic assertion +intersection_of: ECO:0007025 ! indirect Coombs test evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007600 +name: direct hemagglutination assay evidence used in automatic assertion +def: "A type of direct hemagglutination assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007027 {is_inferred="true"} ! direct hemagglutination assay evidence +is_a: ECO:0007596 {is_inferred="true"} ! agglutination test evidence used in automatic assertion +intersection_of: ECO:0007027 ! direct hemagglutination assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007601 +name: viral hemagglutination inhibition assay evidence used in automatic assertion +def: "A type of viral hemagglutination inhibition assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007029 {is_inferred="true"} ! viral hemagglutination inhibition assay evidence +is_a: ECO:0007596 {is_inferred="true"} ! agglutination test evidence used in automatic assertion +intersection_of: ECO:0007029 ! viral hemagglutination inhibition assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007602 +name: compement fixation assay evidence used in automatic assertion +def: "A type of compement fixation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007031 {is_inferred="true"} ! compement fixation assay evidence +is_a: ECO:0007348 {is_inferred="true"} ! immunological assay evidence used in automatic assertion +intersection_of: ECO:0007031 ! compement fixation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007603 +name: neutralization test assay evidence used in automatic assertion +def: "A type of neutralization test assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007033 {is_inferred="true"} ! neutralization test assay evidence +is_a: ECO:0007348 {is_inferred="true"} ! immunological assay evidence used in automatic assertion +intersection_of: ECO:0007033 ! neutralization test assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007604 +name: copper transport assay evidence used in automatic assertion +def: "A type of copper transport assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007035 {is_inferred="true"} ! copper transport assay evidence +is_a: ECO:0007789 {is_inferred="true"} ! transport assay evidence used in automatic assertion +intersection_of: ECO:0007035 ! copper transport assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007605 +name: 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence used in automatic assertion +def: "A type of 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007037 {is_inferred="true"} ! 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence +is_a: ECO:0007798 {is_inferred="true"} ! staining evidence used in automatic assertion +intersection_of: ECO:0007037 ! 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007606 +name: plaque assay evidence used in automatic assertion +def: "A type of plaque assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007039 {is_inferred="true"} ! plaque assay evidence +is_a: ECO:0007778 {is_inferred="true"} ! cell growth assay evidence used in automatic assertion +intersection_of: ECO:0007039 ! plaque assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007607 +name: epifluorescence microscopy evidence used in automatic assertion +def: "A type of epifluorescence microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0006324 {is_inferred="true"} ! fluorescence microscopy evidence used in automatic assertion +is_a: ECO:0007041 {is_inferred="true"} ! epifluorescence microscopy evidence +intersection_of: ECO:0007041 ! epifluorescence microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007608 +name: transmission electron microscopy evidence used in automatic assertion +def: "A type of transmission electron microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007043 {is_inferred="true"} ! transmission electron microscopy evidence +is_a: ECO:0007493 {is_inferred="true"} ! electron microscopy evidence used in automatic assertion +intersection_of: ECO:0007043 ! transmission electron microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007609 +name: scanning electron microscopy evidence used in automatic assertion +def: "A type of scanning electron microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007045 {is_inferred="true"} ! scanning electron microscopy evidence +is_a: ECO:0007493 {is_inferred="true"} ! electron microscopy evidence used in automatic assertion +intersection_of: ECO:0007045 ! scanning electron microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007610 +name: time-lapsed microscopy evidence used in automatic assertion +def: "A type of time-lapsed microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007047 {is_inferred="true"} ! time-lapsed microscopy evidence +is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion +intersection_of: ECO:0007047 ! time-lapsed microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007611 +name: phase contrast microscopy evidence used in automatic assertion +def: "A type of phase contrast microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007049 {is_inferred="true"} ! phase contrast microscopy evidence +is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion +intersection_of: ECO:0007049 ! phase contrast microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007612 +name: transmitted light brightfied mircoscopy evidence used in automatic assertion +def: "A type of transmitted light brightfied mircoscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007051 {is_inferred="true"} ! transmitted light brightfied mircoscopy evidence +is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion +intersection_of: ECO:0007051 ! transmitted light brightfied mircoscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007613 +name: koehler illumination microscopy evidence used in automatic assertion +def: "A type of koehler illumination microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007053 {is_inferred="true"} ! koehler illumination microscopy evidence +is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion +intersection_of: ECO:0007053 ! koehler illumination microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007614 +name: differential interference contrast microscopy evidence used in automatic assertion +def: "A type of differential interference contrast microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007055 {is_inferred="true"} ! differential interference contrast microscopy evidence +is_a: ECO:0007435 {is_inferred="true"} ! microscopy evidence used in automatic assertion +intersection_of: ECO:0007055 ! differential interference contrast microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007615 +name: extended field laser confocal microscopy evidence used in automatic assertion +def: "A type of extended field laser confocal microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007057 {is_inferred="true"} ! extended field laser confocal microscopy evidence +is_a: ECO:0007490 {is_inferred="true"} ! confocal microscopy evidence used in automatic assertion +intersection_of: ECO:0007057 ! extended field laser confocal microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007616 +name: confocal laser scanning microscopy evidence used in automatic assertion +def: "A type of confocal laser scanning microscopy evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007059 {is_inferred="true"} ! confocal laser scanning microscopy evidence +is_a: ECO:0007490 {is_inferred="true"} ! confocal microscopy evidence used in automatic assertion +intersection_of: ECO:0007059 ! confocal laser scanning microscopy evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007617 +name: light scattering evidence used in automatic assertion +def: "A type of light scattering evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007061 {is_inferred="true"} ! light scattering evidence +is_a: ECO:0007815 {is_inferred="true"} ! structure determination evidence used in automatic assertion +intersection_of: ECO:0007061 ! light scattering evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007618 +name: dynamic light scattering assay evidence used in automatic assertion +def: "A type of dynamic light scattering assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007063 {is_inferred="true"} ! dynamic light scattering assay evidence +is_a: ECO:0007617 {is_inferred="true"} ! light scattering evidence used in automatic assertion +intersection_of: ECO:0007063 ! dynamic light scattering assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007619 +name: static light scattering assay evidence used in automatic assertion +def: "A type of static light scattering assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007065 {is_inferred="true"} ! static light scattering assay evidence +is_a: ECO:0007617 {is_inferred="true"} ! light scattering evidence used in automatic assertion +intersection_of: ECO:0007065 ! static light scattering assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007620 +name: colony papillation assay phenotypic evidence used in automatic assertion +def: "A type of colony papillation assay phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "colony papillation assay evidence used in automatic assertion" EXACT [] +is_a: ECO:0007067 {is_inferred="true"} ! colony papillation assay phenotypic evidence +is_a: ECO:0007625 {is_inferred="true"} ! colony morphology phenotypic evidence used in automatic assertion +intersection_of: ECO:0007067 ! colony papillation assay phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007621 +name: crystal violet staining evidence used in automatic assertion +def: "A type of crystal violet staining evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007069 {is_inferred="true"} ! crystal violet staining evidence +is_a: ECO:0007798 {is_inferred="true"} ! staining evidence used in automatic assertion +intersection_of: ECO:0007069 ! crystal violet staining evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007622 +name: flow cell biofilm assay evidence used in automatic assertion +def: "A type of flow cell biofilm assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007071 {is_inferred="true"} ! flow cell biofilm assay evidence +is_a: ECO:0007541 {is_inferred="true"} ! biofilm formation assay evidence used in automatic assertion +intersection_of: ECO:0007071 ! flow cell biofilm assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007623 +name: bacterial 2-hybrid assay evidence used in automatic assertion +def: "A type of bacterial 2-hybrid assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007073 {is_inferred="true"} ! bacterial 2-hybrid assay evidence +is_a: ECO:0007432 {is_inferred="true"} ! bait-prey hybrid interaction evidence used in automatic assertion +intersection_of: ECO:0007073 ! bacterial 2-hybrid assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007624 +name: phenomic profiling assay evidence used in automatic assertion +def: "A type of phenomic profiling assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007075 {is_inferred="true"} ! phenomic profiling assay evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0007075 ! phenomic profiling assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007625 +name: colony morphology phenotypic evidence used in automatic assertion +def: "A type of colony morphology phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "colony morphology evidence used in automatic assertion" EXACT [] +is_a: ECO:0007077 {is_inferred="true"} ! colony morphology phenotypic evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0007077 ! colony morphology phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007626 +name: colony color phenotypic evidence used in automatic assertion +def: "A type of colony color phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "colony color evidence used in automatic assertion" EXACT [] +is_a: ECO:0007079 {is_inferred="true"} ! colony color phenotypic evidence +is_a: ECO:0007625 {is_inferred="true"} ! colony morphology phenotypic evidence used in automatic assertion +intersection_of: ECO:0007079 ! colony color phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007627 +name: colony size phenotypic evidence used in automatic assertion +def: "A type of colony size phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "colony size evidence used in automatic assertion" EXACT [] +is_a: ECO:0007081 {is_inferred="true"} ! colony size phenotypic evidence +is_a: ECO:0007625 {is_inferred="true"} ! colony morphology phenotypic evidence used in automatic assertion +intersection_of: ECO:0007081 ! colony size phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007628 +name: zone of inhibition evidence used in automatic assertion +def: "A type of zone of inhibition evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007083 {is_inferred="true"} ! zone of inhibition evidence +is_a: ECO:0007778 {is_inferred="true"} ! cell growth assay evidence used in automatic assertion +intersection_of: ECO:0007083 ! zone of inhibition evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007629 +name: Etest evidence used in automatic assertion +def: "A type of Etest evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007085 {is_inferred="true"} ! Etest evidence +is_a: ECO:0007628 {is_inferred="true"} ! zone of inhibition evidence used in automatic assertion +intersection_of: ECO:0007085 ! Etest evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007630 +name: ribosome profiling evidence used in automatic assertion +def: "A type of ribosome profiling evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007087 {is_inferred="true"} ! ribosome profiling evidence +is_a: ECO:0007298 {is_inferred="true"} ! expression pattern evidence used in automatic assertion +intersection_of: ECO:0007087 ! ribosome profiling evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007631 +name: computational inference used in manual assertion +def: "A type of evidence based on computational logical inference that is used in a manual assertion." [ECO:RCT] +synonym: "evidence based on computational logical inference used in manual assertion" EXACT [] +is_a: ECO:0000362 {is_inferred="true"} ! computational inference +is_a: ECO:0007668 {is_inferred="true"} ! computational evidence used in manual assertion +is_a: ECO:0007751 {is_inferred="true"} ! inferential evidence used in manual assertion +intersection_of: ECO:0000362 ! computational inference +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-05-14T09:47:55Z + +[Term] +id: ECO:0007632 +name: transcriptional activation assay evidence used in manual assertion +def: "A type of transcriptional activation assay evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000170 {is_inferred="true"} ! transcriptional activation assay evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000170 ! transcriptional activation assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2018-05-18T08:34:35Z + +[Term] +id: ECO:0007633 +name: transcriptional activation assay evidence used in automatic assertion +def: "A type of transcriptional activation assay evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000170 {is_inferred="true"} ! transcriptional activation assay evidence +is_a: ECO:0007768 {is_inferred="true"} ! transcription assay evidence used in automatic assertion +intersection_of: ECO:0000170 ! transcriptional activation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-05-18T08:34:35Z + +[Term] +id: ECO:0007634 +name: experimental phenotypic evidence used in manual assertion +def: "A type of experimental phenotypic evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000059 {is_inferred="true"} ! experimental phenotypic evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +intersection_of: ECO:0000059 ! experimental phenotypic evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2018-06-20T02:12:00Z + +[Term] +id: ECO:0007635 +name: experimental phenotypic evidence used in automatic assertion +def: "A type of experimental phenotypic evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000059 {is_inferred="true"} ! experimental phenotypic evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0000059 ! experimental phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2018-06-20T02:12:00Z + +[Term] +id: ECO:0007636 +name: curator inference from database +def: "A type of curator inference from authoritative resource based on information located in a queryable database and is optimized for computers." [ECO:RCT] +is_a: ECO:0007647 ! curator inference from authoritative source + +[Term] +id: ECO:0007637 +name: curator inference from encyclopedia +def: "A type of curator inference from published work where the reference is to an entry in a compendium that provides summarized information on a subject." [url:https\://en.wikipedia.org/wiki/Encyclopedia] +is_a: ECO:0007647 ! curator inference from authoritative source + +[Term] +id: ECO:0007638 +name: curator inference from Wikipedia +def: "A type of curator inference from encyclopedia where the reference is to a Wikipedia article." [ECO:RCT] +is_a: ECO:0007637 ! curator inference from encyclopedia + +[Term] +id: ECO:0007639 +name: curator inference from Britannica +def: "A type of curator inference from encyclopedia where the reference is to an Encyclopedia Britannica article." [ECO:RCT] +is_a: ECO:0007637 ! curator inference from encyclopedia + +[Term] +id: ECO:0007640 +name: curator inference from MedlinePlus encyclopedia +def: "A type of curator inference from encyclopedia in which the reference is to an article in the National Library of Medicine's MedLinePlus encyclopedia." [ECO:RCT] +is_a: ECO:0007637 ! curator inference from encyclopedia + +[Term] +id: ECO:0007641 +name: curator inference from dictionary +def: "A type of curator inference from published work in which the entry comes from a collection of words with definitions, usages, pronounciations, and more." [url:https\://en.wikipedia.org/wiki/Dictionary] +is_a: ECO:0007647 ! curator inference from authoritative source + +[Term] +id: ECO:0007642 +name: curator inference from Oxford Dictionary +def: "A type of curator inference from dictionary in which the reference is to an entry in the Oxford Dictionaries." [ECO:RCT] +is_a: ECO:0007641 ! curator inference from dictionary + +[Term] +id: ECO:0007643 +name: curator inference from Merriam-Webster Dictionary +def: "A type of curator inference from dictionary in which the reference is to an entry in the Merriam-Webster Dictionary." [ECO:RCT] +is_a: ECO:0007641 ! curator inference from dictionary + +[Term] +id: ECO:0007644 +name: curator inference from MedlinePlus dictionary +def: "A type of curator inference from dictionary in which the reference is to an entry in the National Library of Medicine's MedLinePlus dictionary." [ECO:RCT] +is_a: ECO:0007641 ! curator inference from dictionary + +[Term] +id: ECO:0007645 +name: curator inference from journal publication +def: "A type of curator inference from published work reporting on research findings." [url:https\://en.wikipedia.org/wiki/Scientific_journal] +is_a: ECO:0007647 ! curator inference from authoritative source + +[Term] +id: ECO:0007646 +name: curator inference from book +def: "A type of curator inference from published work based on a book, which may be a reference to a URL (for ebooks) or a DOI." [ECO:RCT] +is_a: ECO:0007647 ! curator inference from authoritative source + +[Term] +id: ECO:0007647 +name: curator inference from authoritative source +def: "A type of curator inference that is from what is generally considered an authoritative source on the topic, including model organism databases, newspaper articles, books, journal publications, etc." [ECO:RCT] +is_a: ECO:0000205 ! curator inference + +[Term] +id: ECO:0007648 +name: manually integrated combinatorial computational evidence +def: "A type of manually integrated combinatorial evidence in which two or more distinct types of computational evidence are integrated manually." [ECO:RCT] +intersection_of: ECO:0007674 ! manually integrated combinatorial evidence +intersection_of: BFO:0000051 ECO:0007672 {all_only="true"} ! computational evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007649 +name: manually integrated combinatorial computational evidence used in manual assertion +def: "A type of manually integrated combinatorial computational evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0007648 {is_inferred="true"} ! manually integrated combinatorial computational evidence +intersection_of: ECO:0007648 ! manually integrated combinatorial computational evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007650 +name: manually integrated combinatorial computational evidence used in automatic assertion +def: "A type of manually integrated combinatorial computational evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +is_a: ECO:0007648 {is_inferred="true"} ! manually integrated combinatorial computational evidence +intersection_of: ECO:0007648 ! manually integrated combinatorial computational evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007651 +name: automatically integrated combinatorial computational evidence +def: "A type of automatically integrated combinatorial evidence in which two or more distinct types of computational evidence are integrated automatically." [ECO:RCT] +intersection_of: ECO:0007673 ! automatically integrated combinatorial evidence +intersection_of: BFO:0000051 ECO:0007672 {all_only="true"} ! computational evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007652 +name: automatically integrated combinatorial computational evidence used in manual assertion +def: "A type of automatically integrated combinatorial computational evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0007651 {is_inferred="true"} ! automatically integrated combinatorial computational evidence +intersection_of: ECO:0007651 ! automatically integrated combinatorial computational evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "RCA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007653 +name: automatically integrated combinatorial computational evidence used in automatic assertion +def: "A type of automatically integrated combinatorial computational evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +is_a: ECO:0007651 {is_inferred="true"} ! automatically integrated combinatorial computational evidence +intersection_of: ECO:0007651 ! automatically integrated combinatorial computational evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007654 +name: combinatorial experimental evidence +def: "A type of combinatorial evidence in which two or more distinct types of experimental evidence are integrated." [ECO:RCT] +intersection_of: ECO:0000212 ! combinatorial evidence +intersection_of: BFO:0000051 ECO:0000006 {all_only="true"} ! experimental evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007655 +name: manually integrated combinatorial experimental evidence +def: "A type of manually integrated combinatorial evidence in which two or more distinct types of experimental evidence are integrated manually." [ECO:RCT] +intersection_of: ECO:0007674 ! manually integrated combinatorial evidence +intersection_of: BFO:0000051 ECO:0000006 {all_only="true"} ! experimental evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007656 +name: manually integrated combinatorial experimental evidence used in manual assertion +def: "A type of manually integrated combinatorial experimental evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0007655 {is_inferred="true"} ! manually integrated combinatorial experimental evidence +intersection_of: ECO:0007655 ! manually integrated combinatorial experimental evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007657 +name: manually integrated combinatorial experimental evidence used in automatic assertion +def: "A type of manually integrated combinatorial experimental evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +is_a: ECO:0007655 {is_inferred="true"} ! manually integrated combinatorial experimental evidence +intersection_of: ECO:0007655 ! manually integrated combinatorial experimental evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007658 +name: automatically integrated combinatorial experimental evidence +def: "A type of automatically integrated combinatorial evidence in which two or more distinct types of experimental evidence are integrated automatically." [ECO:RCT] +intersection_of: ECO:0007673 ! automatically integrated combinatorial evidence +intersection_of: BFO:0000051 ECO:0000006 {all_only="true"} ! experimental evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007659 +name: automatically integrated combinatorial experimental evidence used in manual assertion +def: "A type of automatically integrated combinatorial experimental evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0007658 {is_inferred="true"} ! automatically integrated combinatorial experimental evidence +intersection_of: ECO:0007658 ! automatically integrated combinatorial experimental evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "RCA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007660 +name: automatically integrated combinatorial experimental evidence used in automatic assertion +def: "A type of automatically integrated combinatorial experimental evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +is_a: ECO:0007658 {is_inferred="true"} ! automatically integrated combinatorial experimental evidence +intersection_of: ECO:0007658 ! automatically integrated combinatorial experimental evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007661 +name: combinatorial computational and experimental evidence +def: "A type of combinatorial evidence in which at least one line of experimental evidence and at least one line of computational evidence have been integrated." [ECO:RCT] +is_a: ECO:0000212 {is_inferred="true"} ! combinatorial evidence +intersection_of: ECO:0000212 ! combinatorial evidence +intersection_of: BFO:0000051 ECO:0000006 ! experimental evidence +intersection_of: BFO:0000051 ECO:0007672 ! computational evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007662 +name: manually integrated combinatorial computational and experimental evidence +def: "A type of manually integrated combinatorial evidence in which at least one line of experimental evidence and at least one line of computational evidence have been integrated manually." [ECO:RCT] +is_a: ECO:0007661 {is_inferred="true"} ! combinatorial computational and experimental evidence +is_a: ECO:0007674 {is_inferred="true"} ! manually integrated combinatorial evidence +intersection_of: ECO:0007674 ! manually integrated combinatorial evidence +intersection_of: BFO:0000051 ECO:0000006 ! experimental evidence +intersection_of: BFO:0000051 ECO:0007672 ! computational evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007663 +name: manually integrated combinatorial computational and experimental evidence used in manual assertion +def: "A type of manually integrated combinatorial computational and experimental evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0007662 {is_inferred="true"} ! manually integrated combinatorial computational and experimental evidence +is_a: ECO:0007675 {is_inferred="true"} ! manually integrated combinatorial evidence used in manual assertion +intersection_of: ECO:0007662 ! manually integrated combinatorial computational and experimental evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007664 +name: manually integrated combinatorial computational and experimental evidence used in automatic assertion +def: "A type of manually integrated combinatorial computational and experimental evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0007662 {is_inferred="true"} ! manually integrated combinatorial computational and experimental evidence +is_a: ECO:0007676 {is_inferred="true"} ! manually integrated combinatorial evidence used in automatic assertion +is_a: ECO:0007829 {is_inferred="true"} ! combinatorial computational and experimental evidence used in automatic assertion +intersection_of: ECO:0007662 ! manually integrated combinatorial computational and experimental evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007665 +name: automatically integrated combinatorial computational and experimental evidence +def: "A type of automatically integrated combinatorial evidence in which at least one line of experimental evidence and at least one line of computational evidence have been integrated automatically." [ECO:RCT] +is_a: ECO:0007661 {is_inferred="true"} ! combinatorial computational and experimental evidence +is_a: ECO:0007673 {is_inferred="true"} ! automatically integrated combinatorial evidence +intersection_of: ECO:0007673 ! automatically integrated combinatorial evidence +intersection_of: BFO:0000051 ECO:0000006 ! experimental evidence +intersection_of: BFO:0000051 ECO:0007672 ! computational evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007666 +name: automatically integrated combinatorial computational and experimental evidence used in manual assertion +def: "A type of automatically integrated combinatorial computational and experimental evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000245 {is_inferred="true"} ! automatically integrated combinatorial evidence used in manual assertion +is_a: ECO:0007665 {is_inferred="true"} ! automatically integrated combinatorial computational and experimental evidence +intersection_of: ECO:0007665 ! automatically integrated combinatorial computational and experimental evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "RCA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007667 +name: automatically integrated combinatorial computational and experimental evidence used in automatic assertion +def: "A type of automatically integrated combinatorial computational and experimental evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000053 {is_inferred="true"} ! automatically integrated combinatorial evidence used in automatic assertion +is_a: ECO:0007665 {is_inferred="true"} ! automatically integrated combinatorial computational and experimental evidence +is_a: ECO:0007829 {is_inferred="true"} ! combinatorial computational and experimental evidence used in automatic assertion +intersection_of: ECO:0007665 ! automatically integrated combinatorial computational and experimental evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007668 +name: computational evidence used in manual assertion +def: "A type of computational evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0007672 {is_inferred="true"} ! computational evidence +intersection_of: ECO:0007672 ! computational evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007669 +name: computational evidence used in automatic assertion +def: "A type of computational evidence that is used in an automatic assertion." [ECO:RCT] +synonym: "IEA" EXACT [] +synonym: "inferred from electronic annotation" EXACT [GOECO:IEA] +xref: GO_REF:0000003 "Gene Ontology annotation based on Enzyme Commission mapping." +xref: GO_REF:0000004 "Gene Ontology annotation based on Swiss-Prot keyword mapping." +xref: GO_REF:0000020 "Electronic Gene Ontology annotations created by transferring manual GO annotations between orthologous microbial proteins." +xref: GO_REF:0000041 "IEA (UniProt UniPathway)" +xref: GO_REF:0000043 "Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping." +xref: GO_REF:0000044 "Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt." +xref: GO_REF:0000116 "Automatic Gene Ontology annotation based on Rhea mapping." +xref: GOECO:IEA "inferred from electronic annotation" +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +is_a: ECO:0007672 {is_inferred="true"} ! computational evidence +intersection_of: ECO:0007672 ! computational evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string {comment="Default"} +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007672 +name: computational evidence +def: "A type of evidence in which data are produced, and/or generated, and/or analyzed on a computer." [ECO:RCT] +synonym: "in silico evidence" EXACT [] +is_a: ECO:0000000 ! evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007673 +name: automatically integrated combinatorial evidence +def: "A type of combinatorial evidence in which at least two distinct types of evidence have been integrated automatically." [ECO:RCT] +is_a: ECO:0000212 ! combinatorial evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007674 +name: manually integrated combinatorial evidence +def: "A type of combinatorial evidence in which at least two distinct types of evidence have been integrated manually." [ECO:RCT] +is_a: ECO:0000212 ! combinatorial evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007675 +name: manually integrated combinatorial evidence used in manual assertion +def: "A type of manually integrated combinatorial evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000244 {is_inferred="true"} ! combinatorial evidence used in manual assertion +is_a: ECO:0007674 {is_inferred="true"} ! manually integrated combinatorial evidence +intersection_of: ECO:0007674 ! manually integrated combinatorial evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007676 +name: manually integrated combinatorial evidence used in automatic assertion +def: "A type of manually integrated combinatorial evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000213 {is_inferred="true"} ! combinatorial evidence used in automatic assertion +is_a: ECO:0007674 {is_inferred="true"} ! manually integrated combinatorial evidence +intersection_of: ECO:0007674 ! manually integrated combinatorial evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007677 +name: combinatorial computational evidence +def: "A type of combinatorial evidence in which at least two distinct types of computational evidence have been integrated." [ECO:RCT] +intersection_of: ECO:0000212 ! combinatorial evidence +intersection_of: BFO:0000051 ECO:0007672 {all_only="true"} ! computational evidence +created_by: rctauber +creation_date: 2018-12-04T19:31:00Z + +[Term] +id: ECO:0007678 +name: combinatorial computational evidence used in manual assertion +def: "A type of combinatorial computational evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0007677 {is_inferred="true"} ! combinatorial computational evidence +intersection_of: ECO:0007677 ! combinatorial computational evidence +intersection_of: used_in ECO:0000218 ! manual assertion + +[Term] +id: ECO:0007679 +name: combinatorial computational evidence used in automatic assertion +def: "A type of combinatorial computational evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +is_a: ECO:0007677 {is_inferred="true"} ! combinatorial computational evidence +intersection_of: ECO:0007677 ! combinatorial computational evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string + +[Term] +id: ECO:0007680 +name: chromatography evidence used in manual assertion +def: "A type of chromatography evidence that is used in a manual assertion." [ECO:RCT] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0000325 {is_inferred="true"} ! chromatography evidence +intersection_of: ECO:0000325 ! chromatography evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-01-21T06:28:00Z + +[Term] +id: ECO:0007681 +name: chromatography evidence used in automatic assertion +def: "A type of chromatography evidence that is used in an automatic assertion." [ECO:RCT] +is_a: ECO:0000325 {is_inferred="true"} ! chromatography evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0000325 ! chromatography evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-01-21T06:28:00Z + +[Term] +id: ECO:0007682 +name: reporter gene assay evidence used in manual assertion +def: "A type of reporter gene assay evidence that is used in a manual assertion." [] +is_a: ECO:0000049 ! reporter gene assay evidence +is_a: ECO:0000270 {is_inferred="true"} ! expression pattern evidence used in manual assertion +property_value: ECO:9000002 "IEP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007684 +name: protein separation evidence used in manual assertion +def: "A type of protein separation evidence that is used in a manual assertion." [] +is_a: ECO:0000156 ! protein separation evidence +is_a: ECO:0006005 {is_inferred="true"} ! fractionation evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007685 +name: substance quantification evidence used in manual assertion +def: "A type of substance quantification evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0000335 ! substance quantification evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007686 +name: voltage clamp recording evidence used in manual assertion +def: "A type of voltage clamp recording evidence that is used in a manual assertion." [] +is_a: ECO:0005576 ! voltage clamp recording evidence +is_a: ECO:0006006 {is_inferred="true"} ! electrophysiology assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007687 +name: protein kinase assay evidence used in manual assertion +def: "A type of protein kinase assay evidence that is used in a manual assertion." [] +is_a: ECO:0001558 ! protein kinase assay evidence +is_a: ECO:0005801 {is_inferred="true"} ! enzymatic activity assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007688 +name: gel electrophoresis evidence used in manual assertion +def: "A type of gel electrophoresis evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0000337 ! gel electrophoresis evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007689 +name: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence used in manual assertion +def: "A type of sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007690 +name: ex vivo assay evidence used in manual assertion +def: "A type of ex vivo assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005502 ! ex vivo assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007691 +name: cleavage assay evidence used in manual assertion +def: "A type of cleavage assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001547 ! cleavage assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007692 +name: deacetylation assay evidence used in manual assertion +def: "A type of deacetylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001548 ! deacetylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007693 +name: transcription assay evidence used in manual assertion +def: "A type of transcription assay evidence that is used in a manual assertion." [] +is_a: ECO:0000168 ! transcription assay evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007694 +name: phosphatase assay evidence used in manual assertion +def: "A type of phosphatase assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001556 ! phosphatase assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007695 +name: cell-based assay evidence used in manual assertion +def: "A type of cell-based assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001565 ! cell-based assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007696 +name: cell proliferation assay evidence used in manual assertion +def: "A type of cell proliferation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005007 ! cell proliferation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007697 +name: DNA synthesis cell proliferation assay evidence used in manual assertion +def: "A type of DNA synthesis cell proliferation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005008 ! DNA synthesis cell proliferation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007698 +name: apoptotic assay evidence used in manual assertion +def: "A type of apoptotic assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005034 ! apoptotic assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007699 +name: cell growth assay evidence used in manual assertion +def: "A type of cell growth assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001563 ! cell growth assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007700 +name: disk diffusion test evidence used in manual assertion +def: "A type of disk diffusion test evidence that is used in a manual assertion." [] +is_a: ECO:0005605 ! disk diffusion test evidence +is_a: ECO:0007084 {is_inferred="true"} ! zone of inhibition evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007701 +name: chemotaxis assay evidence used in manual assertion +def: "A type of chemotaxis assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005021 ! chemotaxis assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007702 +name: cytotoxicity assay evidence used in manual assertion +def: "A type of cytotoxicity assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005012 ! cytotoxicity assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007703 +name: cell viability assay evidence used in manual assertion +def: "A type of cell viability assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005004 ! cell viability assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007704 +name: methylation assay evidence used in manual assertion +def: "A type of methylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001554 ! methylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007705 +name: protein assay evidence used in manual assertion +def: "A type of protein assay evidence that is used in a manual assertion." [] +is_obsolete: true +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007706 +name: chromatin immunoprecipitation evidence used in manual assertion +def: "A type of chromatin immunoprecipitation evidence that is used in a manual assertion." [] +is_a: ECO:0000226 ! chromatin immunoprecipitation evidence +is_a: ECO:0005644 {is_inferred="true"} ! immunoprecipitation evidence used in manual assertion +property_value: ECO:9000002 "IPI" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007707 +name: protein inhibition evidence used in manual assertion +def: "A type of protein inhibition evidence that is used in a manual assertion." [] +is_a: ECO:0000020 {is_inferred="true"} ! protein inhibition evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +intersection_of: ECO:0000020 ! protein inhibition evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007708 +name: sumoylation assay evidence used in manual assertion +def: "A type of sumoylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001559 ! sumoylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007709 +name: transport assay evidence used in manual assertion +def: "A type of transport assay evidence that is used in a manual assertion." [] +is_a: ECO:0000134 ! transport assay evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007710 +name: defarnesylation assay evidence used in manual assertion +def: "A type of defarnesylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001549 ! defarnesylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007711 +name: deubiquitination assay evidence used in manual assertion +def: "A type of deubiquitination assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001552 ! deubiquitination assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007712 +name: palmitoylation assay evidence used in manual assertion +def: "A type of palmitoylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001555 ! palmitoylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007713 +name: acetylation assay evidence used in manual assertion +def: "A type of acetylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001546 ! acetylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007714 +name: demethylation assay evidence used in manual assertion +def: "A type of demethylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001550 ! demethylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007715 +name: polyADP-ribosylation assay evidence used in manual assertion +def: "A type of polyADP-ribosylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001557 ! polyADP-ribosylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007716 +name: staining evidence used in manual assertion +def: "A type of staining evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005019 ! staining evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007717 +name: translation assay evidence used in manual assertion +def: "A type of translation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001561 ! translation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007718 +name: ubiquitination assay evidence used in manual assertion +def: "A type of ubiquitination assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001562 ! ubiquitination assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007719 +name: immunodetection assay evidence used in manual assertion +def: "A type of immunodetection assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0005593 ! immunodetection assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007720 +name: desumoylation assay evidence used in manual assertion +def: "A type of desumoylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001551 ! desumoylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007721 +name: farnesylation assay evidence used in manual assertion +def: "A type of farnesylation assay evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001553 ! farnesylation assay evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007722 +name: reconstitution assay evidence used in manual assertion +def: "A type of reconstitution assay evidence that is used in a manual assertion." [] +is_a: ECO:0000003 ! reconstitution assay evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007723 +name: in vitro transcription reconstitution assay evidence used in manual assertion +def: "A type of in vitro transcription reconstitution assay evidence that is used in a manual assertion." [] +is_a: ECO:0000150 ! in vitro transcription reconstitution assay evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007724 +name: localization evidence used in manual assertion +def: "A type of localization evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001533 ! localization evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007725 +name: protein localization evidence used in manual assertion +def: "A type of protein localization evidence that is used in a manual assertion." [] +is_a: ECO:0000122 ! protein localization evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007726 +name: fusion protein localization evidence used in manual assertion +def: "A type of fusion protein localization evidence that is used in a manual assertion." [] +is_a: ECO:0000124 ! fusion protein localization evidence +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007727 +name: nucleic acid localization evidence used in manual assertion +def: "A type of nucleic acid localization evidence that is used in a manual assertion." [] +is_a: ECO:0000314 {is_inferred="true"} ! direct assay evidence used in manual assertion +is_a: ECO:0001534 ! nucleic acid localization evidence +property_value: ECO:9000002 "IDA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007728 +name: anatomical perturbation phenotypic evidence used in manual assertion +def: "A type of anatomical perturbation phenotypic evidence that is used in a manual assertion." [] +is_a: ECO:0000240 ! anatomical perturbation phenotypic evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007730 +name: cleavage arrested development evidence used in manual assertion +def: "A type of cleavage arrested development evidence that is used in a manual assertion." [] +is_a: ECO:0000298 ! cleavage arrested development evidence +is_a: ECO:0007634 {is_inferred="true"} ! experimental phenotypic evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007731 +name: spectrometry evidence used in manual assertion +def: "A type of spectrometry evidence that is used in a manual assertion." [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0005504 ! spectrometry evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007732 +name: sequencing assay evidence used in manual assertion +def: "A type of sequencing assay evidence that is used in a manual assertion." [] +is_a: ECO:0000220 ! sequencing assay evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007733 +name: nucleotide sequencing assay evidence used in manual assertion +def: "A type of nucleotide sequencing assay evidence that is used in a manual assertion." [] +is_a: ECO:0000219 ! nucleotide sequencing assay evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007734 +name: high throughput nucleotide sequencing assay evidence used in manual assertion +def: "A type of high throughput nucleotide sequencing assay evidence that is used in a manual assertion." [] +is_a: ECO:0000221 ! high throughput nucleotide sequencing assay evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007735 +name: structure determination evidence used in manual assertion +def: "A type of structure determination evidence that is used in a manual assertion." [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0005031 ! structure determination evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007736 +name: molecule detection assay evidence used in manual assertion +def: "A type of molecule detection assay evidence that is used in a manual assertion." [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0005516 ! molecule detection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007737 +name: DNA detection assay evidence used in manual assertion +def: "A type of DNA detection assay evidence that is used in a manual assertion." [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0005519 ! DNA detection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007738 +name: protein detection assay evidence used in manual assertion +def: "A type of protein detection assay evidence that is used in a manual assertion." [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0005517 ! protein detection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007739 +name: RNA detection assay evidence used in manual assertion +def: "A type of RNA detection assay evidence that is used in a manual assertion." [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0005518 ! RNA detection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007740 +name: small molecule detection assay evidence used in manual assertion +def: "A type of small molecule detection assay evidence that is used in a manual assertion." [] +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +is_a: ECO:0001522 ! small molecule detection assay evidence +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007741 +name: chromosome conformation-based evidence used in manual assertion +def: "A type of chromosome conformation-based evidence that is used in a manual assertion." [] +is_a: ECO:0000232 ! chromosome conformation-based evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007742 +name: 3C evidence used in manual assertion +def: "A type of 3C evidence that is used in a manual assertion." [] +is_a: ECO:0000233 ! 3C evidence +is_a: ECO:0000269 {is_inferred="true"} ! experimental evidence used in manual assertion +property_value: ECO:9000002 "EXP" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007744 +name: combinatorial computational and experimental evidence used in manual assertion +def: "A type of combinatorial computational and experimental evidence that is used in a manual assertion." [] +is_a: ECO:0000244 {is_inferred="true"} ! combinatorial evidence used in manual assertion +is_a: ECO:0007661 ! combinatorial computational and experimental evidence +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007745 +name: combinatorial experimental evidence used in manual assertion +def: "A type of combinatorial experimental evidence that is used in a manual assertion." [] +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0007654 ! combinatorial experimental evidence +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007746 +name: biological system reconstruction evidence used in manual assertion +def: "A type of biological system reconstruction evidence that is used in a manual assertion." [] +is_a: ECO:0000088 ! biological system reconstruction evidence +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007747 +name: biological system reconstruction evidence by experimental evidence used in manual assertion +def: "A type of biological system reconstruction evidence by experimental evidence that is used in a manual assertion." [] +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0005551 ! biological system reconstruction evidence by experimental evidence +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007748 +name: phenotypic similarity evidence used in manual assertion +def: "A type of phenotypic similarity evidence that is used in a manual assertion." [] +is_a: ECO:0000057 ! phenotypic similarity evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007749 +name: transcript splice pattern evidence used in manual assertion +def: "A type of transcript splice pattern evidence that is used in a manual assertion." [] +is_a: ECO:0000247 {is_inferred="true"} ! sequence alignment evidence used in manual assertion +is_a: ECO:0000326 ! transcript splice pattern evidence +property_value: ECO:9000002 "ISA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007750 +name: phylogenetic evidence used in manual assertion +def: "A type of phylogenetic evidence that is used in a manual assertion." [] +is_a: ECO:0000080 ! phylogenetic evidence +is_a: ECO:0000252 {is_inferred="true"} ! similarity evidence used in manual assertion +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007751 +name: inferential evidence used in manual assertion +def: "A type of inferential evidence that is used in a manual assertion." [] +is_a: ECO:0000352 {is_inferred="true"} ! evidence used in manual assertion +is_a: ECO:0000361 {is_inferred="true"} ! inferential evidence +intersection_of: ECO:0000361 ! inferential evidence +intersection_of: used_in ECO:0000218 ! manual assertion +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007752 +name: curator inference from authoritative source used in manual assertion +def: "A type of curator inference from authoritative source that is used in a manual assertion." [] +is_a: ECO:0000305 {is_inferred="true"} ! curator inference used in manual assertion +is_a: ECO:0007647 {is_inferred="true"} ! curator inference from authoritative source +intersection_of: ECO:0007647 ! curator inference from authoritative source +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007753 +name: curator inference from encyclopedia used in manual assertion +def: "A type of curator inference from encyclopedia that is used in a manual assertion." [] +is_a: ECO:0007637 {is_inferred="true"} ! curator inference from encyclopedia +is_a: ECO:0007752 {is_inferred="true"} ! curator inference from authoritative source used in manual assertion +intersection_of: ECO:0007637 ! curator inference from encyclopedia +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007754 +name: curator inference from Wikipedia used in manual assertion +def: "A type of curator inference from Wikipedia that is used in a manual assertion." [] +is_a: ECO:0007638 {is_inferred="true"} ! curator inference from Wikipedia +is_a: ECO:0007753 {is_inferred="true"} ! curator inference from encyclopedia used in manual assertion +intersection_of: ECO:0007638 ! curator inference from Wikipedia +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007755 +name: curator inference from MedlinePlus encyclopedia used in manual assertion +def: "A type of curator inference from MedlinePlus encyclopedia that is used in a manual assertion." [] +is_a: ECO:0007640 {is_inferred="true"} ! curator inference from MedlinePlus encyclopedia +is_a: ECO:0007753 {is_inferred="true"} ! curator inference from encyclopedia used in manual assertion +intersection_of: ECO:0007640 ! curator inference from MedlinePlus encyclopedia +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007756 +name: curator inference from Britannica used in manual assertion +def: "A type of curator inference from Britannica that is used in a manual assertion." [] +is_a: ECO:0007639 ! curator inference from Britannica +is_a: ECO:0007753 {is_inferred="true"} ! curator inference from encyclopedia used in manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007757 +name: curator inference from book used in manual assertion +def: "A type of curator inference from book that is used in a manual assertion." [] +is_a: ECO:0007646 {is_inferred="true"} ! curator inference from book +is_a: ECO:0007752 {is_inferred="true"} ! curator inference from authoritative source used in manual assertion +intersection_of: ECO:0007646 ! curator inference from book +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007758 +name: curator inference from journal publication used in manual assertion +def: "A type of curator inference from journal publication that is used in a manual assertion." [] +is_a: ECO:0007645 {is_inferred="true"} ! curator inference from journal publication +is_a: ECO:0007752 {is_inferred="true"} ! curator inference from authoritative source used in manual assertion +intersection_of: ECO:0007645 ! curator inference from journal publication +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007759 +name: curator inference from database used in manual assertion +def: "A type of curator inference from database that is used in a manual assertion." [] +is_a: ECO:0007636 {is_inferred="true"} ! curator inference from database +is_a: ECO:0007752 {is_inferred="true"} ! curator inference from authoritative source used in manual assertion +intersection_of: ECO:0007636 ! curator inference from database +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007760 +name: curator inference from dictionary used in manual assertion +def: "A type of curator inference from dictionary that is used in a manual assertion." [] +is_a: ECO:0007641 {is_inferred="true"} ! curator inference from dictionary +is_a: ECO:0007752 {is_inferred="true"} ! curator inference from authoritative source used in manual assertion +intersection_of: ECO:0007641 ! curator inference from dictionary +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007761 +name: curator inference from MedlinePlus dictionary used in manual assertion +def: "A type of curator inference from MedlinePlus dictionary that is used in a manual assertion." [] +is_a: ECO:0007644 {is_inferred="true"} ! curator inference from MedlinePlus dictionary +is_a: ECO:0007760 {is_inferred="true"} ! curator inference from dictionary used in manual assertion +intersection_of: ECO:0007644 ! curator inference from MedlinePlus dictionary +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007762 +name: curator inference from Oxford Dictionary used in manual assertion +def: "A type of curator inference from Oxford Dictionary that is used in a manual assertion." [] +is_a: ECO:0007642 {is_inferred="true"} ! curator inference from Oxford Dictionary +is_a: ECO:0007760 {is_inferred="true"} ! curator inference from dictionary used in manual assertion +intersection_of: ECO:0007642 ! curator inference from Oxford Dictionary +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007763 +name: curator inference from Merriam-Webster Dictionary used in manual assertion +def: "A type of curator inference from Merriam-Webster Dictionary that is used in a manual assertion." [] +is_a: ECO:0007643 {is_inferred="true"} ! curator inference from Merriam-Webster Dictionary +is_a: ECO:0007760 {is_inferred="true"} ! curator inference from dictionary used in manual assertion +intersection_of: ECO:0007643 ! curator inference from Merriam-Webster Dictionary +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IC" xsd:string +created_by: rctauber +creation_date: 2019-03-20T12:27:00Z + +[Term] +id: ECO:0007765 +name: reporter gene assay evidence used in automatic assertion +def: "A type of reporter gene assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000049 {is_inferred="true"} ! reporter gene assay evidence +is_a: ECO:0007298 {is_inferred="true"} ! expression pattern evidence used in automatic assertion +intersection_of: ECO:0000049 ! reporter gene assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007766 +name: voltage clamp recording evidence used in automatic assertion +def: "A type of voltage clamp recording evidence that is used in an automatic assertion." [] +is_a: ECO:0005576 {is_inferred="true"} ! voltage clamp recording evidence +is_a: ECO:0007535 {is_inferred="true"} ! electrophysiology assay evidence used in automatic assertion +intersection_of: ECO:0005576 ! voltage clamp recording evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007767 +name: protein kinase assay evidence used in automatic assertion +def: "A type of protein kinase assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001558 {is_inferred="true"} ! protein kinase assay evidence +is_a: ECO:0007468 {is_inferred="true"} ! enzymatic activity assay evidence used in automatic assertion +intersection_of: ECO:0001558 ! protein kinase assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007768 +name: transcription assay evidence used in automatic assertion +def: "A type of transcription assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000168 {is_inferred="true"} ! transcription assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000168 ! transcription assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007769 +name: gel electrophoresis evidence used in automatic assertion +def: "A type of gel electrophoresis evidence that is used in an automatic assertion." [] +is_a: ECO:0000337 {is_inferred="true"} ! gel electrophoresis evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000337 ! gel electrophoresis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007770 +name: sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence used in automatic assertion +def: "A type of sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence that is used in an automatic assertion." [] +is_a: ECO:0000333 {is_inferred="true"} ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence +is_a: ECO:0007769 {is_inferred="true"} ! gel electrophoresis evidence used in automatic assertion +intersection_of: ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007771 +name: deacetylation assay evidence used in automatic assertion +def: "A type of deacetylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001548 {is_inferred="true"} ! deacetylation assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001548 ! deacetylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007772 +name: phosphatase assay evidence used in automatic assertion +def: "A type of phosphatase assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001556 {is_inferred="true"} ! phosphatase assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001556 ! phosphatase assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007773 +name: cell-based assay evidence used in automatic assertion +def: "A type of cell-based assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001565 {is_inferred="true"} ! cell-based assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001565 ! cell-based assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007774 +name: cell viability assay evidence used in automatic assertion +def: "A type of cell viability assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005004 {is_inferred="true"} ! cell viability assay evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0005004 ! cell viability assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007775 +name: cell proliferation assay evidence used in automatic assertion +def: "A type of cell proliferation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005007 {is_inferred="true"} ! cell proliferation assay evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0005007 ! cell proliferation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007776 +name: DNA synthesis cell proliferation assay evidence used in automatic assertion +def: "A type of DNA synthesis cell proliferation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005008 {is_inferred="true"} ! DNA synthesis cell proliferation assay evidence +is_a: ECO:0007775 {is_inferred="true"} ! cell proliferation assay evidence used in automatic assertion +intersection_of: ECO:0005008 ! DNA synthesis cell proliferation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007777 +name: apoptotic assay evidence used in automatic assertion +def: "A type of apoptotic assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005034 {is_inferred="true"} ! apoptotic assay evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0005034 ! apoptotic assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007778 +name: cell growth assay evidence used in automatic assertion +def: "A type of cell growth assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001563 {is_inferred="true"} ! cell growth assay evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0001563 ! cell growth assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007779 +name: disk diffusion test evidence used in automatic assertion +def: "A type of disk diffusion test evidence that is used in an automatic assertion." [] +is_a: ECO:0005605 {is_inferred="true"} ! disk diffusion test evidence +is_a: ECO:0007628 {is_inferred="true"} ! zone of inhibition evidence used in automatic assertion +intersection_of: ECO:0005605 ! disk diffusion test evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007780 +name: chemotaxis assay evidence used in automatic assertion +def: "A type of chemotaxis assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005021 {is_inferred="true"} ! chemotaxis assay evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0005021 ! chemotaxis assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007781 +name: cytotoxicity assay evidence used in automatic assertion +def: "A type of cytotoxicity assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005012 {is_inferred="true"} ! cytotoxicity assay evidence +is_a: ECO:0007773 {is_inferred="true"} ! cell-based assay evidence used in automatic assertion +intersection_of: ECO:0005012 ! cytotoxicity assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007782 +name: cleavage assay evidence used in automatic assertion +def: "A type of cleavage assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001547 {is_inferred="true"} ! cleavage assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001547 ! cleavage assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007783 +name: methylation assay evidence used in automatic assertion +def: "A type of methylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001554 {is_inferred="true"} ! methylation assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001554 ! methylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007784 +name: protein assay evidence used in automatic assertion +def: "A type of protein assay evidence that is used in an automatic assertion." [] +is_obsolete: true +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007785 +name: protein inhibition evidence used in automatic assertion +def: "A type of protein inhibition evidence that is used in an automatic assertion." [] +is_a: ECO:0000020 {is_inferred="true"} ! protein inhibition evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000020 ! protein inhibition evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007786 +name: chromatin immunoprecipitation evidence used in automatic assertion +def: "A type of chromatin immunoprecipitation evidence that is used in an automatic assertion." [] +is_a: ECO:0000226 {is_inferred="true"} ! chromatin immunoprecipitation evidence +is_a: ECO:0007503 {is_inferred="true"} ! immunoprecipitation evidence used in automatic assertion +intersection_of: ECO:0000226 ! chromatin immunoprecipitation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007787 +name: protein separation evidence used in automatic assertion +def: "A type of protein separation evidence that is used in an automatic assertion." [] +is_a: ECO:0000156 {is_inferred="true"} ! protein separation evidence +is_a: ECO:0007534 {is_inferred="true"} ! fractionation evidence used in automatic assertion +intersection_of: ECO:0000156 ! protein separation evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007788 +name: sumoylation assay evidence used in automatic assertion +def: "A type of sumoylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001559 {is_inferred="true"} ! sumoylation assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001559 ! sumoylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007789 +name: transport assay evidence used in automatic assertion +def: "A type of transport assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000134 {is_inferred="true"} ! transport assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000134 ! transport assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007790 +name: defarnesylation assay evidence used in automatic assertion +def: "A type of defarnesylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001549 {is_inferred="true"} ! defarnesylation assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001549 ! defarnesylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007791 +name: deubiquitination assay evidence used in automatic assertion +def: "A type of deubiquitination assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001552 {is_inferred="true"} ! deubiquitination assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001552 ! deubiquitination assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007792 +name: palmitoylation assay evidence used in automatic assertion +def: "A type of palmitoylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001555 {is_inferred="true"} ! palmitoylation assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001555 ! palmitoylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007793 +name: ex vivo assay evidence used in automatic assertion +def: "A type of ex vivo assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005502 {is_inferred="true"} ! ex vivo assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0005502 ! ex vivo assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007794 +name: acetylation assay evidence used in automatic assertion +def: "A type of acetylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001546 {is_inferred="true"} ! acetylation assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001546 ! acetylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007795 +name: demethylation assay evidence used in automatic assertion +def: "A type of demethylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001550 {is_inferred="true"} ! demethylation assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001550 ! demethylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007796 +name: immunodetection assay evidence used in automatic assertion +def: "A type of immunodetection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005593 {is_inferred="true"} ! immunodetection assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0005593 ! immunodetection assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007797 +name: polyADP-ribosylation assay evidence used in automatic assertion +def: "A type of polyADP-ribosylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001557 {is_inferred="true"} ! polyADP-ribosylation assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001557 ! polyADP-ribosylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007798 +name: staining evidence used in automatic assertion +def: "A type of staining evidence that is used in an automatic assertion." [] +is_a: ECO:0005019 {is_inferred="true"} ! staining evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0005019 ! staining evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007799 +name: translation assay evidence used in automatic assertion +def: "A type of translation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001561 {is_inferred="true"} ! translation assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001561 ! translation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007800 +name: farnesylation assay evidence used in automatic assertion +def: "A type of farnesylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001553 {is_inferred="true"} ! farnesylation assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001553 ! farnesylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007801 +name: ubiquitination assay evidence used in automatic assertion +def: "A type of ubiquitination assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001562 {is_inferred="true"} ! ubiquitination assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001562 ! ubiquitination assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007802 +name: desumoylation assay evidence used in automatic assertion +def: "A type of desumoylation assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001551 {is_inferred="true"} ! desumoylation assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001551 ! desumoylation assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007803 +name: reconstitution assay evidence used in automatic assertion +def: "A type of reconstitution assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000003 {is_inferred="true"} ! reconstitution assay evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000003 ! reconstitution assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007804 +name: in vitro transcription reconstitution assay evidence used in automatic assertion +def: "A type of in vitro transcription reconstitution assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000150 {is_inferred="true"} ! in vitro transcription reconstitution assay evidence +is_a: ECO:0007803 {is_inferred="true"} ! reconstitution assay evidence used in automatic assertion +intersection_of: ECO:0000150 ! in vitro transcription reconstitution assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007805 +name: substance quantification evidence used in automatic assertion +def: "A type of substance quantification evidence that is used in an automatic assertion." [] +is_a: ECO:0000335 {is_inferred="true"} ! substance quantification evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0000335 ! substance quantification evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007806 +name: localization evidence used in automatic assertion +def: "A type of localization evidence that is used in an automatic assertion." [] +is_a: ECO:0001533 {is_inferred="true"} ! localization evidence +is_a: ECO:0007307 {is_inferred="true"} ! direct assay evidence used in automatic assertion +intersection_of: ECO:0001533 ! localization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007807 +name: protein localization evidence used in automatic assertion +def: "A type of protein localization evidence that is used in an automatic assertion." [] +is_a: ECO:0000122 {is_inferred="true"} ! protein localization evidence +is_a: ECO:0007806 {is_inferred="true"} ! localization evidence used in automatic assertion +intersection_of: ECO:0000122 ! protein localization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007808 +name: fusion protein localization evidence used in automatic assertion +def: "A type of fusion protein localization evidence that is used in an automatic assertion." [] +is_a: ECO:0000124 {is_inferred="true"} ! fusion protein localization evidence +is_a: ECO:0007807 {is_inferred="true"} ! protein localization evidence used in automatic assertion +intersection_of: ECO:0000124 ! fusion protein localization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007809 +name: nucleic acid localization evidence used in automatic assertion +def: "A type of nucleic acid localization evidence that is used in an automatic assertion." [] +is_a: ECO:0001534 {is_inferred="true"} ! nucleic acid localization evidence +is_a: ECO:0007806 {is_inferred="true"} ! localization evidence used in automatic assertion +intersection_of: ECO:0001534 ! nucleic acid localization evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007810 +name: anatomical perturbation phenotypic evidence used in automatic assertion +def: "A type of anatomical perturbation phenotypic evidence that is used in an automatic assertion." [] +is_a: ECO:0000240 {is_inferred="true"} ! anatomical perturbation phenotypic evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0000240 ! anatomical perturbation phenotypic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007811 +name: cleavage arrested development evidence used in automatic assertion +def: "A type of cleavage arrested development evidence that is used in an automatic assertion." [] +is_a: ECO:0000298 {is_inferred="true"} ! cleavage arrested development evidence +is_a: ECO:0007635 {is_inferred="true"} ! experimental phenotypic evidence used in automatic assertion +intersection_of: ECO:0000298 ! cleavage arrested development evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007812 +name: sequencing assay evidence used in automatic assertion +def: "A type of sequencing assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000220 {is_inferred="true"} ! sequencing assay evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0000220 ! sequencing assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007813 +name: nucleotide sequencing assay evidence used in automatic assertion +def: "A type of nucleotide sequencing assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000219 {is_inferred="true"} ! nucleotide sequencing assay evidence +is_a: ECO:0007812 {is_inferred="true"} ! sequencing assay evidence used in automatic assertion +intersection_of: ECO:0000219 ! nucleotide sequencing assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007814 +name: high throughput nucleotide sequencing assay evidence used in automatic assertion +def: "A type of high throughput nucleotide sequencing assay evidence that is used in an automatic assertion." [] +is_a: ECO:0000221 {is_inferred="true"} ! high throughput nucleotide sequencing assay evidence +is_a: ECO:0007813 {is_inferred="true"} ! nucleotide sequencing assay evidence used in automatic assertion +intersection_of: ECO:0000221 ! high throughput nucleotide sequencing assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007815 +name: structure determination evidence used in automatic assertion +def: "A type of structure determination evidence that is used in an automatic assertion." [] +is_a: ECO:0005031 {is_inferred="true"} ! structure determination evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0005031 ! structure determination evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007816 +name: molecule detection assay evidence used in automatic assertion +def: "A type of molecule detection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005516 {is_inferred="true"} ! molecule detection assay evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0005516 ! molecule detection assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007817 +name: DNA detection assay evidence used in automatic assertion +def: "A type of DNA detection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005519 {is_inferred="true"} ! DNA detection assay evidence +is_a: ECO:0007816 {is_inferred="true"} ! molecule detection assay evidence used in automatic assertion +intersection_of: ECO:0005519 ! DNA detection assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007818 +name: RNA detection assay evidence used in automatic assertion +def: "A type of RNA detection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005518 {is_inferred="true"} ! RNA detection assay evidence +is_a: ECO:0007816 {is_inferred="true"} ! molecule detection assay evidence used in automatic assertion +intersection_of: ECO:0005518 ! RNA detection assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007819 +name: protein detection assay evidence used in automatic assertion +def: "A type of protein detection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0005517 {is_inferred="true"} ! protein detection assay evidence +is_a: ECO:0007816 {is_inferred="true"} ! molecule detection assay evidence used in automatic assertion +intersection_of: ECO:0005517 ! protein detection assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007820 +name: small molecule detection assay evidence used in automatic assertion +def: "A type of small molecule detection assay evidence that is used in an automatic assertion." [] +is_a: ECO:0001522 {is_inferred="true"} ! small molecule detection assay evidence +is_a: ECO:0007816 {is_inferred="true"} ! molecule detection assay evidence used in automatic assertion +intersection_of: ECO:0001522 ! small molecule detection assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007821 +name: spectrometry evidence used in automatic assertion +def: "A type of spectrometry evidence that is used in an automatic assertion." [] +is_a: ECO:0005504 {is_inferred="true"} ! spectrometry evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0005504 ! spectrometry evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007822 +name: chromosome conformation-based evidence used in automatic assertion +def: "A type of chromosome conformation-based evidence that is used in an automatic assertion." [] +is_a: ECO:0000232 {is_inferred="true"} ! chromosome conformation-based evidence +is_a: ECO:0007297 {is_inferred="true"} ! experimental evidence used in automatic assertion +intersection_of: ECO:0000232 ! chromosome conformation-based evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007823 +name: 3C evidence used in automatic assertion +def: "A type of 3C evidence that is used in an automatic assertion." [] +is_a: ECO:0000233 {is_inferred="true"} ! 3C evidence +is_a: ECO:0007822 {is_inferred="true"} ! chromosome conformation-based evidence used in automatic assertion +intersection_of: ECO:0000233 ! 3C evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007824 +name: phenotypic similarity evidence used in automatic assertion +def: "A type of phenotypic similarity evidence that is used in an automatic assertion." [] +is_a: ECO:0000057 {is_inferred="true"} ! phenotypic similarity evidence +is_a: ECO:0000251 {is_inferred="true"} ! similarity evidence used in automatic assertion +intersection_of: ECO:0000057 ! phenotypic similarity evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007825 +name: transcript splice pattern evidence used in automatic assertion +def: "A type of transcript splice pattern evidence that is used in an automatic assertion." [] +is_a: ECO:0000248 {is_inferred="true"} ! sequence alignment evidence used in automatic assertion +is_a: ECO:0000326 {is_inferred="true"} ! transcript splice pattern evidence +intersection_of: ECO:0000326 ! transcript splice pattern evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007826 +name: phylogenetic evidence used in automatic assertion +def: "A type of phylogenetic evidence that is used in an automatic assertion." [] +is_a: ECO:0000080 {is_inferred="true"} ! phylogenetic evidence +is_a: ECO:0000251 {is_inferred="true"} ! similarity evidence used in automatic assertion +intersection_of: ECO:0000080 ! phylogenetic evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007827 +name: biological system reconstruction evidence used in automatic assertion +def: "A type of biological system reconstruction evidence that is used in an automatic assertion." [] +is_a: ECO:0000088 {is_inferred="true"} ! biological system reconstruction evidence +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +intersection_of: ECO:0000088 ! biological system reconstruction evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007828 +name: biological system reconstruction evidence by experimental evidence used in automatic assertion +def: "A type of biological system reconstruction evidence by experimental evidence that is used in an automatic assertion." [] +is_a: ECO:0005551 {is_inferred="true"} ! biological system reconstruction evidence by experimental evidence +is_a: ECO:0007827 {is_inferred="true"} ! biological system reconstruction evidence used in automatic assertion +intersection_of: ECO:0005551 ! biological system reconstruction evidence by experimental evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007829 +name: combinatorial computational and experimental evidence used in automatic assertion +def: "A type of combinatorial computational and experimental evidence that is used in an automatic assertion." [] +is_a: ECO:0000213 {is_inferred="true"} ! combinatorial evidence used in automatic assertion +is_a: ECO:0007661 {is_inferred="true"} ! combinatorial computational and experimental evidence +intersection_of: ECO:0007661 ! combinatorial computational and experimental evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007830 +name: combinatorial experimental evidence used in automatic assertion +def: "A type of combinatorial experimental evidence that is used in an automatic assertion." [] +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +is_a: ECO:0007654 {is_inferred="true"} ! combinatorial experimental evidence +intersection_of: ECO:0007654 ! combinatorial experimental evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007832 +name: inferential evidence used in automatic assertion +def: "A type of inferential evidence that is used in an automatic assertion." [] +is_a: ECO:0000361 {is_inferred="true"} ! inferential evidence +is_a: ECO:0000501 {is_inferred="true"} ! evidence used in automatic assertion +intersection_of: ECO:0000361 ! inferential evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007833 +name: curator inference from authoritative source used in automatic assertion +def: "A type of curator inference from authoritative source that is used in an automatic assertion." [] +is_a: ECO:0007322 {is_inferred="true"} ! curator inference used in automatic assertion +is_a: ECO:0007647 {is_inferred="true"} ! curator inference from authoritative source +intersection_of: ECO:0007647 ! curator inference from authoritative source +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007834 +name: curator inference from encyclopedia used in automatic assertion +def: "A type of curator inference from encyclopedia that is used in an automatic assertion." [] +is_a: ECO:0007637 {is_inferred="true"} ! curator inference from encyclopedia +is_a: ECO:0007833 {is_inferred="true"} ! curator inference from authoritative source used in automatic assertion +intersection_of: ECO:0007637 ! curator inference from encyclopedia +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007835 +name: curator inference from Wikipedia used in automatic assertion +def: "A type of curator inference from Wikipedia that is used in an automatic assertion." [] +is_a: ECO:0007638 {is_inferred="true"} ! curator inference from Wikipedia +is_a: ECO:0007834 {is_inferred="true"} ! curator inference from encyclopedia used in automatic assertion +intersection_of: ECO:0007638 ! curator inference from Wikipedia +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007836 +name: curator inference from Britannica used in automatic assertion +def: "A type of curator inference from Britannica that is used in an automatic assertion." [] +is_a: ECO:0007639 {is_inferred="true"} ! curator inference from Britannica +is_a: ECO:0007834 {is_inferred="true"} ! curator inference from encyclopedia used in automatic assertion +intersection_of: ECO:0007639 ! curator inference from Britannica +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007837 +name: curator inference from MedlinePlus encyclopedia used in automatic assertion +def: "A type of curator inference from MedlinePlus encyclopedia that is used in an automatic assertion." [] +is_a: ECO:0007640 {is_inferred="true"} ! curator inference from MedlinePlus encyclopedia +is_a: ECO:0007834 {is_inferred="true"} ! curator inference from encyclopedia used in automatic assertion +intersection_of: ECO:0007640 ! curator inference from MedlinePlus encyclopedia +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007838 +name: curator inference from book used in automatic assertion +def: "A type of curator inference from book that is used in an automatic assertion." [] +is_a: ECO:0007646 {is_inferred="true"} ! curator inference from book +is_a: ECO:0007833 {is_inferred="true"} ! curator inference from authoritative source used in automatic assertion +intersection_of: ECO:0007646 ! curator inference from book +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007839 +name: curator inference from dictionary used in automatic assertion +def: "A type of curator inference from dictionary that is used in an automatic assertion." [] +is_a: ECO:0007641 {is_inferred="true"} ! curator inference from dictionary +is_a: ECO:0007833 {is_inferred="true"} ! curator inference from authoritative source used in automatic assertion +intersection_of: ECO:0007641 ! curator inference from dictionary +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007840 +name: curator inference from MedlinePlus dictionary used in automatic assertion +def: "A type of curator inference from MedlinePlus dictionary that is used in an automatic assertion." [] +is_a: ECO:0007644 {is_inferred="true"} ! curator inference from MedlinePlus dictionary +is_a: ECO:0007839 {is_inferred="true"} ! curator inference from dictionary used in automatic assertion +intersection_of: ECO:0007644 ! curator inference from MedlinePlus dictionary +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007841 +name: curator inference from Merriam-Webster Dictionary used in automatic assertion +def: "A type of curator inference from Merriam-Webster Dictionary that is used in an automatic assertion." [] +is_a: ECO:0007643 {is_inferred="true"} ! curator inference from Merriam-Webster Dictionary +is_a: ECO:0007839 {is_inferred="true"} ! curator inference from dictionary used in automatic assertion +intersection_of: ECO:0007643 ! curator inference from Merriam-Webster Dictionary +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007842 +name: curator inference from Oxford Dictionary used in automatic assertion +def: "A type of curator inference from Oxford Dictionary that is used in an automatic assertion." [] +is_a: ECO:0007642 {is_inferred="true"} ! curator inference from Oxford Dictionary +is_a: ECO:0007839 {is_inferred="true"} ! curator inference from dictionary used in automatic assertion +intersection_of: ECO:0007642 ! curator inference from Oxford Dictionary +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007843 +name: curator inference from journal publication used in automatic assertion +def: "A type of curator inference from journal publication that is used in an automatic assertion." [] +is_a: ECO:0007645 {is_inferred="true"} ! curator inference from journal publication +is_a: ECO:0007833 {is_inferred="true"} ! curator inference from authoritative source used in automatic assertion +intersection_of: ECO:0007645 ! curator inference from journal publication +intersection_of: used_in ECO:0000203 ! automatic assertion +property_value: ECO:9000002 "IEA" xsd:string +created_by: rctauber +creation_date: 2019-03-20T13:02:00Z + +[Term] +id: ECO:0007844 +name: radioisotope quantification assay evidence +def: "A type of radioisotope assay evidence in which radioisotopic labeling is used to quantify a substance after a biochemical process or extraction from a biological sample." [] +is_a: ECO:0001120 ! radioisotope assay evidence +property_value: IAO:0000234 "dosumis" xsd:string +created_by: rcjackson +creation_date: 2019-06-12T10:46:00Z + +[Term] +id: ECO:0007845 +name: radioisotope quantification assay evidence used in automatic assertion +def: "A type of radioisotope quantification assay evidence that is used in an automatic assertion." [] +is_a: ECO:0007455 {is_inferred="true"} ! radioisotope assay evidence used in automatic assertion +is_a: ECO:0007844 ! radioisotope quantification assay evidence +intersection_of: ECO:0007844 ! radioisotope quantification assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rcjackson +creation_date: 2019-06-12T10:46:00Z + +[Term] +id: ECO:0007846 +name: radioisotope quantification assay evidence used in manual assertion +def: "A type of radioisotope quantification assay evidence that is used in a manual assertion." [] +is_a: ECO:0001254 {is_inferred="true"} ! radioisotope assay evidence used in manual assertion +is_a: ECO:0007844 ! radioisotope quantification assay evidence +intersection_of: ECO:0007844 ! radioisotope quantification assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rcjackson +creation_date: 2019-06-12T10:46:00Z + +[Term] +id: ECO:0007847 +name: fluorescence quantification assay evidence +def: "A type of fluorescence evidence in which fluorescent labeling is used to quantify a substance after a biochemical process or extraction from a biological sample." [] +is_a: ECO:0001115 ! fluorescence evidence +created_by: rcjackson +creation_date: 2019-06-12T10:46:00Z + +[Term] +id: ECO:0007848 +name: fluorescence quantification assay evidence used in automatic assertion +def: "A type of fluorescence quantification assay evidence that is used in an automatic assertion." [] +is_a: ECO:0007450 {is_inferred="true"} ! fluorescence evidence used in automatic assertion +is_a: ECO:0007847 ! fluorescence quantification assay evidence +intersection_of: ECO:0007847 ! fluorescence quantification assay evidence +intersection_of: used_in ECO:0000203 ! automatic assertion +created_by: rcjackson +creation_date: 2019-06-12T10:46:00Z + +[Term] +id: ECO:0007849 +name: fluorescence quantification assay evidence used in manual assertion +def: "A type of fluorescence quantification assay evidence that is used in a manual assertion." [] +is_a: ECO:0001249 {is_inferred="true"} ! fluorescence evidence used in manual assertion +is_a: ECO:0007847 ! fluorescence quantification assay evidence +intersection_of: ECO:0007847 ! fluorescence quantification assay evidence +intersection_of: used_in ECO:0000218 ! manual assertion +property_value: ECO:9000002 "IDA" xsd:string +created_by: rcjackson +creation_date: 2019-06-12T10:46:00Z + +[Term] +id: ECO:0007850 +name: inference of sequence features from visual inspection +def: "A type of inferential evidence in which sequence features are inferred based on visual inspection of the sequence." [ECO:RCJ] +comment: This type of inference may be made by either a curator or an author. +is_a: ECO:0000361 ! inferential evidence +created_by: beckyjackson +creation_date: 2019-11-22T06:51:00Z + +[Typedef] +id: used_in +name: used_in +def: "A relation connecting a piece of evidence to an assertion method, where that assertion method is supported by the evidence." [GOC:cjm] +comment: In the future we may use a more generic relation with weaker domain and range constraints taken from IAO, RO or OBI. +xref: ECO:9000000 +domain: ECO:0000000 ! evidence +range: ECO:0000217 ! assertion method +inverse_of: uses ! uses +created_by: mchibucos +creation_date: 2010-12-09T05:00:20Z + +[Typedef] +id: uses +name: uses +xref: ECO:9000001 +