diff --git a/specfile/conditions.py b/specfile/conditions.py
index 257ac92..e56c2e8 100644
--- a/specfile/conditions.py
+++ b/specfile/conditions.py
@@ -82,7 +82,7 @@ def process_conditions(
     if macro_definitions:
         for md in macro_definitions:
             position = md.get_position(macro_definitions)
-            excluded_lines.append(range(position, position + len(md.body.splitlines())))
+            excluded_lines.append(range(position, position + len(md.body.split("\n"))))
     condition_regex = re.compile(
         r"""
         ^
diff --git a/specfile/macro_definitions.py b/specfile/macro_definitions.py
index 68a4316..9f136c9 100644
--- a/specfile/macro_definitions.py
+++ b/specfile/macro_definitions.py
@@ -156,11 +156,7 @@ def get_raw_data(self) -> List[str]:
             elif self.comment_out_style is CommentOutStyle.HASH:
                 sc = "#"
         macro = "global" if self.is_global else "define"
-        body = self.body.splitlines()
-        if body:
-            body[-1] += ws[3]
-        else:
-            body = [ws[3]]
+        body = (self.body + ws[3]).split("\n")
         result.append(f"{ws[0]}{dnl}{pre}{sc}{macro}{ws[1]}{self.name}{ws[2]}{body[0]}")
         result.extend(body[1:])
         return result
@@ -383,7 +379,7 @@ def count_brackets(s):
                         line, _ = pop(lines)
                         body += "\n" + line
                         bc, pc = count_brackets(body)
-                tokens = re.split(r"(\s+)$", body, maxsplit=1)
+                tokens = re.split(r"([^\S\n]+)$", body, maxsplit=1)
                 if len(tokens) == 1:
                     body = tokens[0]
                 else:
diff --git a/specfile/sections.py b/specfile/sections.py
index 1057e88..7b5ae98 100644
--- a/specfile/sections.py
+++ b/specfile/sections.py
@@ -268,7 +268,7 @@ def split_id(line):
         macro_definitions = MacroDefinitions.parse(lines)
         for md in macro_definitions:
             position = md.get_position(macro_definitions)
-            excluded_lines.append(range(position, position + len(md.body.splitlines())))
+            excluded_lines.append(range(position, position + len(md.body.split("\n"))))
         section_id_regexes = [
             re.compile(rf"^%{re.escape(n)}(\s+.*(?<!\\)$|$)", re.IGNORECASE)
             for n in SECTION_NAMES
diff --git a/tests/unit/test_conditions.py b/tests/unit/test_conditions.py
index a26654e..2fba827 100644
--- a/tests/unit/test_conditions.py
+++ b/tests/unit/test_conditions.py
@@ -6,6 +6,7 @@
 
 import specfile.conditions
 from specfile.conditions import process_conditions
+from specfile.macro_definitions import MacroDefinitions
 
 
 @pytest.mark.parametrize(
@@ -77,3 +78,8 @@ def resolve_expression(kwd, exp, *_, **__):
     processed_lines, processed_validity = zip(*process_conditions(lines))
     assert list(processed_lines) == lines
     assert list(processed_validity) == validity
+    processed_lines, processed_validity = zip(
+        *process_conditions(lines, MacroDefinitions.parse(lines))
+    )
+    assert list(processed_lines) == lines
+    assert list(processed_validity) == validity
diff --git a/tests/unit/test_macro_definitions.py b/tests/unit/test_macro_definitions.py
index 98976cd..d3e5b2d 100644
--- a/tests/unit/test_macro_definitions.py
+++ b/tests/unit/test_macro_definitions.py
@@ -101,6 +101,9 @@ def test_parse():
             "%define desc(x) Test spec file containing several \\",
             "macro definitions in various formats (%?1)",
             "",
+            "%global trailing_newline \\",
+            "body with trailing newline \\",
+            "",
             "%define example() %{expand:",
             "This an example of a macro definition with body ",
             "spawning across mutiple lines}",
@@ -124,6 +127,12 @@ def test_parse():
         "Test spec file containing several \\\n"
         "macro definitions in various formats (%?1)"
     )
+    assert macro_definitions[7].name == "trailing_newline"
+    assert macro_definitions[7].body == "\\\nbody with trailing newline \\\n"
+    assert macro_definitions[7].is_global
+    assert not macro_definitions[7].commented_out
+    assert macro_definitions[7]._whitespace == ("", " ", " ", "")
+    assert macro_definitions[7].valid
     assert macro_definitions[-1].name == "example()"
     assert macro_definitions[-1].body == (
         "%{expand:\n"
@@ -220,6 +229,14 @@ def test_get_raw_data():
                 True,
                 [""],
             ),
+            MacroDefinition(
+                "trailing_newline",
+                "\\\nbody with trailing newline \\\n",
+                True,
+                False,
+                CommentOutStyle.DNL,
+                ("", " ", " ", ""),
+            ),
         ]
     )
     assert macro_definitions.get_raw_data() == [
@@ -240,6 +257,9 @@ def test_get_raw_data():
         "%define example() %{expand:",
         "This an example of a macro definition with body ",
         "spawning across mutiple lines}",
+        "%global trailing_newline \\",
+        "body with trailing newline \\",
+        "",
     ]
 
 
diff --git a/tests/unit/test_sections.py b/tests/unit/test_sections.py
index 8fc9f18..ccc0736 100644
--- a/tests/unit/test_sections.py
+++ b/tests/unit/test_sections.py
@@ -6,6 +6,7 @@
 import pytest
 from flexmock import flexmock
 
+from specfile.macro_definitions import MacroDefinitions
 from specfile.options import Options, Token, TokenType
 from specfile.sections import Section, Sections
 
@@ -104,32 +105,35 @@ def test_parse_invalid_name():
 
 
 def test_parse_macro_definitions():
-    sections = Sections.parse(
-        [
-            "%package -n test",
-            "Summary: Subpackage test",
-            "",
-            "%description -n test",
-            "Subpackage test.",
-            "",
-            "%define template1()\\",
-            "%package -n %{1}\\",
-            "Summary: Subpackage %{1}\\",
-            "\\",
-            "%description -n %{1}\\",
-            "Subpackage %{1}.",
-            "",
-            "%define template2() %{expand:",
-            "%package -n %{1}",
-            "Summary: Subpackage %{1}",
-            "",
-            "%description -n %{1}",
-            "Subpackage %{1}.}",
-            "",
-            "%prep",
-            "%autosetup",
-        ]
-    )
+    lines = [
+        "%package -n test",
+        "Summary: Subpackage test",
+        "",
+        "%description -n test",
+        "Subpackage test.",
+        "",
+        "%define template1()\\",
+        "%package -n %{1}\\",
+        "Summary: Subpackage %{1}\\",
+        "\\",
+        "%description -n %{1}\\",
+        "Subpackage %{1}.",
+        "",
+        "%define template2() %{expand:",
+        "%package -n %{1}",
+        "Summary: Subpackage %{1}",
+        "",
+        "%description -n %{1}",
+        "Subpackage %{1}.}",
+        "",
+        "%prep",
+        "%autosetup",
+    ]
+    sections = Sections.parse(lines)
+    assert len(sections) == 4
+    assert sections[1].id == "package -n test"
+    assert sections[-1].id == "prep"
+    sections = Sections.parse(lines, MacroDefinitions.parse(lines))
     assert len(sections) == 4
     assert sections[1].id == "package -n test"
     assert sections[-1].id == "prep"