diff --git a/mypy/expandtype.py b/mypy/expandtype.py
index ec6a2ecfd0d2..f7fa0258f588 100644
--- a/mypy/expandtype.py
+++ b/mypy/expandtype.py
@@ -26,6 +26,7 @@
     Type,
     TypeAliasType,
     TypedDictType,
+    TypeOfAny,
     TypeType,
     TypeVarId,
     TypeVarLikeType,
@@ -312,24 +313,26 @@ def interpolate_args_for_unpack(self, t: CallableType, var_arg: UnpackType) -> l
         suffix = self.expand_types(t.arg_types[star_index + 1 :])
 
         var_arg_type = get_proper_type(var_arg.type)
+        new_unpack: Type
         if isinstance(var_arg_type, Instance):
             # we have something like Unpack[Tuple[Any, ...]]
             new_unpack = var_arg
-        else:
-            if isinstance(var_arg_type, TupleType):
-                # We have something like Unpack[Tuple[Unpack[Ts], X1, X2]]
-                expanded_tuple = var_arg_type.accept(self)
-                assert isinstance(expanded_tuple, ProperType) and isinstance(
-                    expanded_tuple, TupleType
-                )
-                expanded_items = expanded_tuple.items
-                fallback = var_arg_type.partial_fallback
-            else:
-                # We have plain Unpack[Ts]
-                assert isinstance(var_arg_type, TypeVarTupleType), type(var_arg_type)
-                fallback = var_arg_type.tuple_fallback
-                expanded_items = self.expand_unpack(var_arg)
+        elif isinstance(var_arg_type, TupleType):
+            # We have something like Unpack[Tuple[Unpack[Ts], X1, X2]]
+            expanded_tuple = var_arg_type.accept(self)
+            assert isinstance(expanded_tuple, ProperType) and isinstance(expanded_tuple, TupleType)
+            expanded_items = expanded_tuple.items
+            fallback = var_arg_type.partial_fallback
             new_unpack = UnpackType(TupleType(expanded_items, fallback))
+        elif isinstance(var_arg_type, TypeVarTupleType):
+            # We have plain Unpack[Ts]
+            fallback = var_arg_type.tuple_fallback
+            expanded_items = self.expand_unpack(var_arg)
+            new_unpack = UnpackType(TupleType(expanded_items, fallback))
+        else:
+            # We have invalid type in Unpack. This can happen when expanding aliases
+            # to Callable[[*Invalid], Ret]
+            new_unpack = AnyType(TypeOfAny.from_error, line=var_arg.line, column=var_arg.column)
         return prefix + [new_unpack] + suffix
 
     def visit_callable_type(self, t: CallableType) -> CallableType:
diff --git a/test-data/unit/check-python311.test b/test-data/unit/check-python311.test
index 37dc3ca0f5b4..2d1a09ef3336 100644
--- a/test-data/unit/check-python311.test
+++ b/test-data/unit/check-python311.test
@@ -142,3 +142,34 @@ myclass3 = MyClass(float, float, float)  # E: No overload variant of "MyClass" m
                                          # N:     def [T1, T2] __init__(Type[T1], Type[T2], /) -> MyClass[T1, T2]
 reveal_type(myclass3)  # N: Revealed type is "Any"
 [builtins fixtures/tuple.pyi]
+
+[case testUnpackNewSyntaxInvalidCallableAlias]
+from typing import Any, Callable, List, Tuple, TypeVar, Unpack
+
+T = TypeVar("T")
+Ts = TypeVarTuple("Ts")  # E: Name "TypeVarTuple" is not defined
+
+def good(*x: int) -> int: ...
+def bad(*x: int, y: int) -> int: ...
+
+Alias1 = Callable[[*Ts], int]  # E: Variable "__main__.Ts" is not valid as a type \
+                               # N: See https://mypy.readthedocs.io/en/stable/common_issues.html#variables-vs-type-aliases
+x1: Alias1[int]  # E: Bad number of arguments for type alias, expected 0, given 1
+reveal_type(x1)  # N: Revealed type is "def (*Any) -> builtins.int"
+x1 = good
+x1 = bad  # E: Incompatible types in assignment (expression has type "Callable[[VarArg(int), NamedArg(int, 'y')], int]", variable has type "Callable[[VarArg(Any)], int]")
+
+Alias2 = Callable[[*T], int]  # E: "T" cannot be unpacked (must be tuple or TypeVarTuple)
+x2: Alias2[int]
+reveal_type(x2)  # N: Revealed type is "def (*Any) -> builtins.int"
+
+Unknown = Any
+Alias3 = Callable[[*Unknown], int]
+x3: Alias3[int]  # E: Bad number of arguments for type alias, expected 0, given 1
+reveal_type(x3)  # N: Revealed type is "def (*Any) -> builtins.int"
+
+IntList = List[int]
+Alias4 = Callable[[*IntList], int]  # E: "List[int]" cannot be unpacked (must be tuple or TypeVarTuple)
+x4: Alias4[int]  # E: Bad number of arguments for type alias, expected 0, given 1
+reveal_type(x4)  # N: Revealed type is "def (*Unpack[builtins.tuple[Any, ...]]) -> builtins.int"
+[builtins fixtures/tuple.pyi]
diff --git a/test-data/unit/check-python312.test b/test-data/unit/check-python312.test
index 188c51f98185..2b99a42628b1 100644
--- a/test-data/unit/check-python312.test
+++ b/test-data/unit/check-python312.test
@@ -76,10 +76,9 @@ BadAlias1 = TypeAliasType("BadAlias1", tuple[*Ts])  # E: TypeVarTuple "Ts" is no
 ba1: BadAlias1[int]  # E: Bad number of arguments for type alias, expected 0, given 1
 reveal_type(ba1)  # N: Revealed type is "builtins.tuple[Any, ...]"
 
-# TODO this should report errors on the two following lines
-#BadAlias2 = TypeAliasType("BadAlias2", Callable[[*Ts], str])
-#ba2: BadAlias2[int]
-#reveal_type(ba2)
+BadAlias2 = TypeAliasType("BadAlias2", Callable[[*Ts], str])  # E: TypeVarTuple "Ts" is not included in type_params
+ba2: BadAlias2[int]  # E: Bad number of arguments for type alias, expected 0, given 1
+reveal_type(ba2)  # N: Revealed type is "def (*Any) -> builtins.str"
 
 [builtins fixtures/tuple.pyi]
 [typing fixtures/typing-full.pyi]
diff --git a/test-data/unit/check-type-aliases.test b/test-data/unit/check-type-aliases.test
index a9c57d46ad22..aebb0381d962 100644
--- a/test-data/unit/check-type-aliases.test
+++ b/test-data/unit/check-type-aliases.test
@@ -1190,10 +1190,9 @@ Ta9 = TypeAliasType("Ta9", Callable[P, T])  # E: ParamSpec "P" is not included i
 unbound_ps_alias3: Ta9[int, str]  # E: Bad number of arguments for type alias, expected 0, given 2
 reveal_type(unbound_ps_alias3)  # N: Revealed type is "def [P] (*Any, **Any) -> Any"
 
-# TODO this should report errors on the two following lines
-#Ta10 = TypeAliasType("Ta10", Callable[[Unpack[Ts]], str])
-#unbound_tvt_alias2: Ta10[int]
-#reveal_type(unbound_tvt_alias2)
+Ta10 = TypeAliasType("Ta10", Callable[[Unpack[Ts]], str])  # E: TypeVarTuple "Ts" is not included in type_params
+unbound_tvt_alias2: Ta10[int]  # E: Bad number of arguments for type alias, expected 0, given 1
+reveal_type(unbound_tvt_alias2)  # N: Revealed type is "def (*Any) -> builtins.str"
 
 class A(Generic[T]):
     Ta11 = TypeAliasType("Ta11", Dict[str, T], type_params=(T,))  # E: Can't use bound type variable "T" to define generic alias \
diff --git a/test-data/unit/check-typevar-tuple.test b/test-data/unit/check-typevar-tuple.test
index cc3dc4ed9f39..f704e3c5c713 100644
--- a/test-data/unit/check-typevar-tuple.test
+++ b/test-data/unit/check-typevar-tuple.test
@@ -2278,6 +2278,22 @@ higher_order(bad3)  # E: Argument 1 to "higher_order" has incompatible type "Cal
 higher_order(bad4)  # E: Argument 1 to "higher_order" has incompatible type "Callable[[KwArg(None)], None]"; expected "Callable[[int, str, VarArg(Unpack[Tuple[Unpack[Tuple[Any, ...]], int]])], Any]"
 [builtins fixtures/tuple.pyi]
 
+[case testAliasToCallableWithUnpackInvalid]
+from typing import Any, Callable, List, Tuple, TypeVar, Unpack
+
+T = TypeVar("T")
+Ts = TypeVarTuple("Ts")  # E: Name "TypeVarTuple" is not defined
+
+def good(*x: int) -> int: ...
+def bad(*x: int, y: int) -> int: ...
+
+Alias = Callable[[Unpack[T]], int]  # E: "T" cannot be unpacked (must be tuple or TypeVarTuple)
+x: Alias[int]
+reveal_type(x)  # N: Revealed type is "def (*Any) -> builtins.int"
+x = good
+x = bad  # E: Incompatible types in assignment (expression has type "Callable[[VarArg(int), NamedArg(int, 'y')], int]", variable has type "Callable[[VarArg(Any)], int]")
+[builtins fixtures/tuple.pyi]
+
 [case testTypeVarTupleInvariant]
 from typing import Generic, Tuple
 from typing_extensions import Unpack, TypeVarTuple