diff --git a/qmake/generators/mac/pbuilder_pbx.cpp b/qmake/generators/mac/pbuilder_pbx.cpp
index f03dce2bc406..292e2cfbda13 100644
--- a/qmake/generators/mac/pbuilder_pbx.cpp
+++ b/qmake/generators/mac/pbuilder_pbx.cpp
@@ -1875,7 +1875,7 @@ ProjectBuilderMakefileGenerator::findProgram(const ProString &prog)
 {
     QString ret = prog.toQString();
     if(QDir::isRelativePath(ret)) {
-        QStringList paths = QString(qgetenv("PATH")).split(':');
+        QStringList paths = QString(qgetenv("PATH")).split(QDir::listSeparator());
         for(int i = 0; i < paths.size(); ++i) {
             QString path = paths.at(i) + "/" + prog;
             if(exists(path)) {
diff --git a/qmake/library/ioutils.cpp b/qmake/library/ioutils.cpp
index c46eb7b6638c..df1a66263f02 100644
--- a/qmake/library/ioutils.cpp
+++ b/qmake/library/ioutils.cpp
@@ -63,11 +63,9 @@ QString IoUtils::binaryAbsLocation(const QString &argv0)
     } else { // in the PATH
         QByteArray pEnv = qgetenv("PATH");
         QDir currentDir = QDir::current();
+        QStringList paths = QString::fromLocal8Bit(pEnv).split(QDir::listSeparator());
 #ifdef Q_OS_WIN
-        QStringList paths = QString::fromLocal8Bit(pEnv).split(QLatin1String(";"));
         paths.prepend(QLatin1String("."));
-#else
-        QStringList paths = QString::fromLocal8Bit(pEnv).split(QLatin1String(":"));
 #endif
         for (QStringList::const_iterator p = paths.constBegin(); p != paths.constEnd(); ++p) {
             if ((*p).isEmpty())
diff --git a/qmake/library/qmakeevaluator.cpp b/qmake/library/qmakeevaluator.cpp
index 3b0ff8ee9340..c158bc975103 100644
--- a/qmake/library/qmakeevaluator.cpp
+++ b/qmake/library/qmakeevaluator.cpp
@@ -1023,7 +1023,7 @@ static ProString msvcArchitecture(const QString &vcInstallDir, const QString &pa
     QString vcBinDir = vcInstallDir;
     if (vcBinDir.endsWith(QLatin1Char('\\')))
         vcBinDir.chop(1);
-    const auto dirs = pathVar.split(QLatin1Char(';'), Qt::SkipEmptyParts);
+    const auto dirs = pathVar.split(QDir::listSeparator(), Qt::SkipEmptyParts);
     for (const QString &dir : dirs) {
         if (!dir.startsWith(vcBinDir, Qt::CaseInsensitive))
             continue;
diff --git a/qmake/library/qmakeglobals.cpp b/qmake/library/qmakeglobals.cpp
index 7d51b0f18420..d7fb440b37ea 100644
--- a/qmake/library/qmakeglobals.cpp
+++ b/qmake/library/qmakeglobals.cpp
@@ -78,13 +78,8 @@ QMakeGlobals::QMakeGlobals()
 #ifdef PROEVALUATOR_DEBUG
     debugLevel = 0;
 #endif
-#ifdef Q_OS_WIN
-    dirlist_sep = QLatin1Char(';');
-    dir_sep = QLatin1Char('\\');
-#else
-    dirlist_sep = QLatin1Char(':');
-    dir_sep = QLatin1Char('/');
-#endif
+dirlist_sep = QDir::listSeparator();
+dir_sep = QDir::separator();
 }
 
 QMakeGlobals::~QMakeGlobals()
diff --git a/src/corelib/plugin/qsystemlibrary.cpp b/src/corelib/plugin/qsystemlibrary.cpp
index 593b0c3be703..a95a438a210d 100644
--- a/src/corelib/plugin/qsystemlibrary.cpp
+++ b/src/corelib/plugin/qsystemlibrary.cpp
@@ -101,7 +101,7 @@ HINSTANCE QSystemLibrary::load(const wchar_t *libraryName, bool onlySystemDirect
 
     if (!onlySystemDirectory) {
         const QString PATH(QLatin1String(qgetenv("PATH").constData()));
-        searchOrder << PATH.split(QLatin1Char(';'), Qt::SkipEmptyParts);
+        searchOrder << PATH.split(QDir::listSeparator(), Qt::SkipEmptyParts);
     }
     QString fileName = QString::fromWCharArray(libraryName);
     fileName.append(QLatin1String(".dll"));
@@ -109,8 +109,8 @@ HINSTANCE QSystemLibrary::load(const wchar_t *libraryName, bool onlySystemDirect
     // Start looking in the order specified
     for (int i = 0; i < searchOrder.count(); ++i) {
         QString fullPathAttempt = searchOrder.at(i);
-        if (!fullPathAttempt.endsWith(QLatin1Char('\\'))) {
-            fullPathAttempt.append(QLatin1Char('\\'));
+        if (!fullPathAttempt.endsWith(QDir::separator())) {
+            fullPathAttempt.append(QDir::separator());
         }
         fullPathAttempt.append(fileName);
         HINSTANCE inst = ::LoadLibrary(reinterpret_cast<const wchar_t *>(fullPathAttempt.utf16()));
diff --git a/src/tools/androiddeployqt/main.cpp b/src/tools/androiddeployqt/main.cpp
index 766af0ec313e..651962e400c0 100644
--- a/src/tools/androiddeployqt/main.cpp
+++ b/src/tools/androiddeployqt/main.cpp
@@ -2499,13 +2499,7 @@ bool createAndroidProject(const Options &options)
 QString findInPath(const QString &fileName)
 {
     const QString path = QString::fromLocal8Bit(qgetenv("PATH"));
-#if defined(Q_OS_WIN32)
-    QLatin1Char separator(';');
-#else
-    QLatin1Char separator(':');
-#endif
-
-    const QStringList paths = path.split(separator);
+    const QStringList paths = path.split(QDir::listSeparator());
     for (const QString &path : paths) {
         QFileInfo fileInfo(path + QLatin1Char('/') + fileName);
         if (fileInfo.exists() && fileInfo.isFile() && fileInfo.isExecutable())
diff --git a/src/tools/windeployqt/main.cpp b/src/tools/windeployqt/main.cpp
index 27b57ea9ad38..1de1a560ab82 100644
--- a/src/tools/windeployqt/main.cpp
+++ b/src/tools/windeployqt/main.cpp
@@ -1648,7 +1648,7 @@ int main(int argc, char **argv)
     const QByteArray qtBinPath = QFile::encodeName(QDir::toNativeSeparators(QCoreApplication::applicationDirPath()));
     QByteArray path = qgetenv("PATH");
     if (!path.contains(qtBinPath)) { // QTBUG-39177, ensure Qt is in the path so that qt.conf is taken into account.
-        path += ';';
+        path += QDir::listSeparator();
         path += qtBinPath;
         qputenv("PATH", path);
     }
diff --git a/src/tools/windeployqt/utils.cpp b/src/tools/windeployqt/utils.cpp
index bc52de924e41..a5809022ea90 100644
--- a/src/tools/windeployqt/utils.cpp
+++ b/src/tools/windeployqt/utils.cpp
@@ -172,7 +172,7 @@ QString normalizeFileName(const QString &name)
 // Find a tool binary in the Windows SDK 8
 QString findSdkTool(const QString &tool)
 {
-    QStringList paths = QString::fromLocal8Bit(qgetenv("PATH")).split(QLatin1Char(';'));
+    QStringList paths = QString::fromLocal8Bit(qgetenv("PATH")).split(QDir::listSeparator());
     const QByteArray sdkDir = qgetenv("WindowsSdkDir");
     if (!sdkDir.isEmpty())
         paths.prepend(QDir::cleanPath(QString::fromLocal8Bit(sdkDir)) + QLatin1String("/Tools/x64"));
diff --git a/tests/auto/tools/windeployqt/tst_windeployqt.cpp b/tests/auto/tools/windeployqt/tst_windeployqt.cpp
index 38403c90fca1..446984cb641f 100644
--- a/tests/auto/tools/windeployqt/tst_windeployqt.cpp
+++ b/tests/auto/tools/windeployqt/tst_windeployqt.cpp
@@ -149,7 +149,7 @@ void tst_windeployqt::deploy()
     const QString qtBinDir = QDir::toNativeSeparators(QLibraryInfo::path(QLibraryInfo::BinariesPath));
     QProcessEnvironment env = QProcessEnvironment::systemEnvironment();
     const QString pathKey = QLatin1String("PATH");
-    const QChar pathSeparator(QLatin1Char(';')); // ### fixme: Qt 5.6: QDir::listSeparator()
+    const QChar pathSeparator(QDir::listSeparator());
     const QString origPath = env.value(pathKey);
     QString newPath;
     const QStringList pathElements = origPath.split(pathSeparator, Qt::SkipEmptyParts);