diff --git a/primerschemes/quick-chikv-ecsa/300/v1.0.0/README.md b/primerschemes/quick-chikv-ecsa/300/v1.0.0/README.md new file mode 100644 index 0000000..0c7c373 --- /dev/null +++ b/primerschemes/quick-chikv-ecsa/300/v1.0.0/README.md @@ -0,0 +1,57 @@ +# quick-chikv-ecsa-300 300bp v1.0.0 + +[![Generic badge](https://img.shields.io/badge/STATUS-draft-blue.svg)](https://labs.primalscheme.com/faqs) + +> If you use this scheme please cite: Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples. doi:10.1038/nprot.2017.066 + +[primalscheme labs](https://labs.primalscheme.com/detail/quick-chikv-ecsa-300/300/v1.0.0) + +## Overviews + +## Details + +```json +{ + "ampliconsize": 300, + "schemeversion": "v1.0.0", + "schemename": "quick-chikv-ecsa-300", + "primer_bed_md5": "69837908bf4d89963193fc2c673fcea5", + "reference_fasta_md5": "7f06dfa1bf98cd8bc7ed4b86ec000b40", + "status": "draft", + "citations": [ + "Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples. doi:10.1038/nprot.2017.066" + ], + "authors": [ + "quick-lab", + "loman-lab" + ], + "algorithmversion": "ps:100", + "species": [ + 37124 + ], + "license": "CC BY-SA 4.0", + "primerclass": "primerschemes", + "infoschema": "v2.1.0", + "articbedversion": "v3.0", + "collections": [], + "links": { + "protocols": [], + "validation": [], + "homepage": [], + "vendors": [], + "misc": [] + }, + "refselect": null, + "description": null, + "derivedfrom": null, + "contactinfo": null +} +``` + + + +------------------------------------------------------------------------ + +This work is licensed under a [Creative Commons Attribution-ShareAlike 4.0 International License](http://creativecommons.org/licenses/by-sa/4.0/) + +![](https://i.creativecommons.org/l/by-sa/4.0/88x31.png) \ No newline at end of file diff --git a/primerschemes/quick-chikv-ecsa/300/v1.0.0/info.json b/primerschemes/quick-chikv-ecsa/300/v1.0.0/info.json new file mode 100644 index 0000000..087a0ef --- /dev/null +++ b/primerschemes/quick-chikv-ecsa/300/v1.0.0/info.json @@ -0,0 +1,35 @@ +{ + "ampliconsize": 300, + "schemeversion": "v1.0.0", + "schemename": "quick-chikv-ecsa", + "primer_bed_md5": "69837908bf4d89963193fc2c673fcea5", + "reference_fasta_md5": "7f06dfa1bf98cd8bc7ed4b86ec000b40", + "status": "validated", + "citations": [ + "Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples. doi:10.1038/nprot.2017.066" + ], + "authors": [ + "quick-lab", + "loman-lab" + ], + "algorithmversion": "ps:100", + "species": [ + 37124 + ], + "license": "CC BY-SA 4.0", + "primerclass": "primerschemes", + "infoschema": "v2.1.0", + "articbedversion": "v3.0", + "collections": [], + "links": { + "protocols": [], + "validation": [], + "homepage": [], + "vendors": [], + "misc": [] + }, + "refselect": null, + "description": null, + "derivedfrom": null, + "contactinfo": null +} \ No newline at end of file diff --git a/primerschemes/quick-chikv-ecsa/300/v1.0.0/primer.bed b/primerschemes/quick-chikv-ecsa/300/v1.0.0/primer.bed new file mode 100644 index 0000000..ea93489 --- /dev/null +++ b/primerschemes/quick-chikv-ecsa/300/v1.0.0/primer.bed @@ -0,0 +1,89 @@ +# artic-bed-version v3.0 +KP164568.1 115 137 CHIK400_1_LEFT_3 1 + CTTTTTGAAGGCCCTGCAACGT +KP164568.1 428 451 CHIK400_1_RIGHT_3 1 - ACGGCCATCACCTCTTGTAAGT +KP164568.1 294 316 CHIK400_2_LEFT_0 2 + TGTCGGACAGGAAGTACCACTG +KP164568.1 660 685 CHIK400_2_RIGHT_0 2 - CTCATCCGCCCAATTTGTCGAG +KP164568.1 595 617 CHIK400_3_LEFT_0 1 + GTACTGGGTAGGGTTCGACACA +KP164568.1 902 924 CHIK400_3_RIGHT_0 1 - CACACGAAACCACTGTGTCACA +KP164568.1 860 882 CHIK400_4_LEFT_4 2 + TCGGTGTTCCATCTAAAGGGCA +KP164568.1 1195 1217 CHIK400_4_RIGHT_4 2 - CTTCATGGTGTTCGTGTTCCGT +KP164568.1 1153 1175 CHIK400_5_LEFT_0 1 + GGTGGGGCTGAACCAGAGAATA +KP164568.1 1408 1430 CHIK400_5_RIGHT_0 1 - TACAAAGCTGTCGAATTCGGCC +KP164568.1 1349 1371 CHIK400_6_LEFT_4 2 + AAGCAGAAAACACACACGGTCT +KP164568.1 1686 1710 CHIK400_6_RIGHT_4 2 - GCGCCTCTCGGAGTCTCTATTA +KP164568.1 1614 1636 CHIK400_7_LEFT_0 1 + TACAGGCAGCACAGGAAGATGT +KP164568.1 1908 1930 CHIK400_7_RIGHT_0 1 - TGGAAGTCTTCGGGCGAAATTG +KP164568.1 1816 1838 CHIK400_8_LEFT_1 2 + GGAGCAAGTGAAGACGTGTACG +KP164568.1 2192 2214 CHIK400_8_RIGHT_1 2 - TATATGGACAAGCGGGGCGAAT +KP164568.1 2105 2127 CHIK400_9_LEFT_0 1 + TGCAAGAAGGAAGAAGCTGCAG +KP164568.1 2463 2485 CHIK400_9_RIGHT_0 1 - AAGGCGATCAAGGCAAGTAACG +KP164568.1 2368 2390 CHIK400_10_LEFT_0 2 + ATCTGCACGTACGGTTGATTCG +KP164568.1 2709 2731 CHIK400_10_RIGHT_0 2 - GGTTTTGTTGAGCCCGTAGTGT +KP164568.1 2663 2685 CHIK400_11_LEFT_0 1 + GGCAAAATGCGCACTACGAATG +KP164568.1 2916 2938 CHIK400_11_RIGHT_0 1 - CCTTCCGTACGCGTTAGGAGTA +KP164568.1 2845 2868 CHIK400_12_LEFT_1 2 + AGGAGTTTACGCAGTTAGGCAAA +KP164568.1 3220 3242 CHIK400_12_RIGHT_1 2 - TTCATTCAGGGCTACTTCGGGT +KP164568.1 3129 3151 CHIK400_13_LEFT_0 1 + TGGTCCCTATCCTCGAAACAGC +KP164568.1 3431 3453 CHIK400_13_RIGHT_0 1 - TCCTGGTAGTCACGCAGATCTG +KP164568.1 3367 3389 CHIK400_14_LEFT_0 2 + TGAGGCAGCGTCCATTCTAGAA +KP164568.1 3697 3719 CHIK400_14_RIGHT_0 2 - ATACCTACCAAGTGTTGCCGGT +KP164568.1 3609 3632 CHIK400_15_LEFT_4 1 + ACCTTGCACTGCCTACTAAGAGA +KP164568.1 3921 3947 CHIK400_15_RIGHT_4 1 - ACATGGTGGTTTCAATGCTCTGG +KP164568.1 3852 3874 CHIK400_16_LEFT_0 2 + TCAGAGCATACGGTTACGCAGA +KP164568.1 4221 4243 CHIK400_16_RIGHT_0 2 - TTTGCGGTTCCTACTGGTGTTG +KP164568.1 4166 4188 CHIK400_17_LEFT_0 1 + GACGGTGTTTGCAAGGCAGTAT +KP164568.1 4452 4474 CHIK400_17_RIGHT_0 1 - TCCATGGCTGTAAAGAGGTGGT +KP164568.1 4393 4415 CHIK400_18_LEFT_0 2 + ACCTCTCCTCTCCACAGGTGTA +KP164568.1 4731 4753 CHIK400_18_RIGHT_0 2 - GCCTCTATTTGCTTTGGCCACA +KP164568.1 4680 4702 CHIK400_19_LEFT_0 1 + AAGGGACACGTTTTCACCAGAC +KP164568.1 4970 4994 CHIK400_19_RIGHT_0 1 - GGAGCATTTGACTTTTTGCACTCC +KP164568.1 4898 4920 CHIK400_20_LEFT_0 2 + CGACTTCGCATGAACCATGTCA +KP164568.1 5187 5209 CHIK400_20_RIGHT_0 2 - GTATGTATCGCCCCGTCGTCTA +KP164568.1 4301 4332 CHIK400_21_LEFT_0 1 + AGTTTGATCTAAGCGCCGATGG +KP164568.1 5491 5513 CHIK400_21_RIGHT_0 1 - AAATGTGATGGGGAACGTCTCG +KP164568.1 5406 5429 CHIK400_22_LEFT_0 2 + CGCGTGACACAGCTATTTCCTT +KP164568.1 5700 5722 CHIK400_22_RIGHT_0 2 - TGGCGTACCGACTTCTGTTGTA +KP164568.1 5628 5650 CHIK400_23_LEFT_0 1 + ACACGGACGACGAGTTATGACT +KP164568.1 5991 6013 CHIK400_23_RIGHT_0 1 - GACAATCGGACGTTGATCGGAG +KP164568.1 5928 5950 CHIK400_24_LEFT_0 2 + TAATGGCAGAGACCCCGAAAGT +KP164568.1 6226 6248 CHIK400_24_RIGHT_0 2 - TGTGTTCTGGAATGGGGAAGGT +KP164568.1 6146 6168 CHIK400_25_LEFT_0 1 + CGAGCGACATTCAATCCGTCAA +KP164568.1 6475 6497 CHIK400_25_RIGHT_0 1 - CGGCAGCAGATTATGGGTTCTT +KP164568.1 6380 6402 CHIK400_26_LEFT_3 2 + GAAGAATTTGCTGCCAGCCCTA +KP164568.1 6722 6744 CHIK400_26_RIGHT_3 2 - GTGCGGCTATAATGGCATCGAA +KP164568.1 6657 6679 CHIK400_27_LEFT_0 1 + TTAGGAGATTGAACGCCGTCCT +KP164568.1 7008 7033 CHIK400_27_RIGHT_0 1 - GTCAGACGATCTTCCAACACCC +KP164568.1 6916 6938 CHIK400_28_LEFT_2 2 + GACAGGTACGCGCTTCAAGTTC +KP164568.1 7254 7277 CHIK400_28_RIGHT_2 2 - CGCTAACGGTTTGCCCAGTTTA +KP164568.1 7168 7191 CHIK400_29_LEFT_0 1 + AGCTCCCTACTTTTGTGGAGGG +KP164568.1 7472 7494 CHIK400_29_RIGHT_0 1 - GACCGCCGTACAAGGTTATGAC +KP164568.1 7413 7435 CHIK400_30_LEFT_0 2 + TAATGTCCATGGCCACCTTTGC +KP164568.1 7747 7769 CHIK400_30_RIGHT_0 2 - TATTCTTCCGATTCTTGCGCGG +KP164568.1 7684 7706 CHIK400_31_LEFT_0 1 + CAACTTGCCCAGCTGATCTCAG +KP164568.1 7980 8002 CHIK400_31_RIGHT_0 1 - ATCGATGGTCCCCTTTACGTGT +KP164568.1 7929 7951 CHIK400_32_LEFT_0 2 + TAAGGTAACAGGTTACGCGTGC +KP164568.1 8257 8279 CHIK400_32_RIGHT_0 2 - TACGGGCTCCTTCATTAGCTCC +KP164568.1 8210 8232 CHIK400_33_LEFT_2 1 + GACCGATCTTCGACAACAAGGG +KP164568.1 8510 8532 CHIK400_33_RIGHT_2 1 - GCGTCGGGGAGAACATGTTAAG +KP164568.1 8441 8465 CHIK400_34_LEFT_0 2 + GAGAAAACCTTGCGCATGCTTG +KP164568.1 8749 8771 CHIK400_34_RIGHT_0 2 - CTGCTGGCATGTGATTGTCCAT +KP164568.1 8685 8707 CHIK400_35_LEFT_0 1 + CCAGGTTTCCTTGCAAATCGGA +KP164568.1 8983 9010 CHIK400_35_RIGHT_0 1 - GTACGTGCTGCAAGGTAGTTCC +KP164568.1 8912 8934 CHIK400_36_LEFT_1 2 + CATGTACGCACCCATTTCACCA +KP164568.1 9270 9293 CHIK400_36_RIGHT_1 2 - GGTCCCCGAATTCAGCATTACG +KP164568.1 9198 9220 CHIK400_37_LEFT_0 1 + CTGCAAGGTCGATCAATGCCAT +KP164568.1 9521 9543 CHIK400_37_RIGHT_0 1 - TCATTGTTACCCCACGTGACCT +KP164568.1 9467 9489 CHIK400_38_LEFT_0 2 + AGTGGGTGACGCATAAGAAGGA +KP164568.1 9731 9754 CHIK400_38_RIGHT_0 2 - TGGTGTCAGTTCGTACGGTGTA +KP164568.1 9670 9692 CHIK400_39_LEFT_0 1 + ATACTCCTGTCGATGGTGGGTG +KP164568.1 9996 10018 CHIK400_39_RIGHT_0 1 - CGGGATCACTGTTACGTGTTCG +KP164568.1 9952 9974 CHIK400_40_LEFT_0 2 + TTTTTAGCCGTACTGAGCGTCG +KP164568.1 10272 10294 CHIK400_40_RIGHT_0 2 - ATTTTCGGTGTCGCAGAAGCAG +KP164568.1 10181 10203 CHIK400_41_LEFT_0 1 + GCGGTACAGCAGAGTGTAAGGA +KP164568.1 10520 10542 CHIK400_41_RIGHT_0 1 - GTCGCCTTTGTACACCACGATT +KP164568.1 10446 10468 CHIK400_42_LEFT_0 2 + CCATGCCGTCACAGTTAAGGAC +KP164568.1 10782 10804 CHIK400_42_RIGHT_0 2 - AGTTCATCGCTCTTACCGGGTT +KP164568.1 10717 10739 CHIK400_43_LEFT_0 1 + ATTGGCTAAAAGAACGAGGGGC +KP164568.1 10994 11016 CHIK400_43_RIGHT_0 1 - TTCCCGAATAGTGACGGCGTTA +KP164568.1 10909 10931 CHIK400_44_LEFT_0 2 + GCACCCATTCCTCAGACTTTGG +KP164568.1 11285 11307 CHIK400_44_RIGHT_0 2 - CCTGCTAAACGACACGCATAGC diff --git a/primerschemes/quick-chikv-ecsa/300/v1.0.0/reference.fasta b/primerschemes/quick-chikv-ecsa/300/v1.0.0/reference.fasta new file mode 100644 index 0000000..7ce678c --- /dev/null +++ b/primerschemes/quick-chikv-ecsa/300/v1.0.0/reference.fasta @@ -0,0 +1,198 @@ +>KP164568.1 Chikungunya virus isolate BHI3734/H804698, complete genome +GTAGGCTACGTGTGACACACGTAGCCTACCAGTTTCTTACTGCTCTACTCTGCAAAGCAA +GAGATTAAGAACCCATCATGGATCCTGTGTACGTGGACATAGACGCTGACAGCGCCTTTT +TGAAGGCCCTGCAACGTGCGTACCCCATGTTTGAGGTGGAACCCAGGCAGGTCACACCGA +ATGACCATGCTAATGCTAGAGCGTTCTCGCATCTAGCTATAAAACTAATAGAGCAGGAAA +TTGATCCCGACTCAACCATCCTGGACATCGGCAGTGCACCAGCAAGGAGGATGATGTCGG +ACAGGAAGTACCACTGCGTCTGCCCGATGCGCAGCGCAGAAGATCCCGAGAGACTCGCCA +ATTATGCGAGAAAGCTAGCATCTGCTGCAGGAAAAGTCCTGGACAGAAACATCTCTGGAA +AGATCGGGGACTTACAAGCAGTAATGGCCGTGCCAGACACGGAGACGCCAACATTCTGCT +TGCACACAGATGTCTCATGTAGACAGAGAGCAGACGTCGCGATATACCAAGACGTCTATG +CTGTACACGCACCCACGTCGCTATACCACCAGGCGATTAAAGGGGTCCGAGTGGCGTACT +GGGTAGGGTTCGACACAACCCCGTTCATGTACAATGCCATGGCGGGTGCCTACCCCTCAT +ATTCGACAAACTGGGCAGACGAGCAGGTACTGAAGGCTAAGAACATAGGATTATGTTCAA +CAGACCTGACGGAAGGTAGACGAGGTAAATTGTCTATTATGAGAGGGAAGAAGCTAAAAC +CGTGTGACCGTGTGCTGTTCTCAGTAGGGTCAACGCTCTACCCGGAAAGCCGCAAGCTAC +TTAAGAGCTGGCACTTACCATCGGTGTTCCATCTAAAGGGCAAACTCAGCTTCACATGCC +GCTGTGATACAGTGGTTTCGTGCGAGGGCTACGTCGTTAAGAGAATAACGATGAGCCCAG +GCCTTTATGGAAAAACCACAGGGTATGCGGTAACCCACCACGCAGACGGATTCTTGATGT +GCAAGACTACCGACACGGTTGACGGCGAAAGAGTGTCATTCTCGGTGTGCACATACGTGC +CGGCGACCATTTGTGATCAAATGACCGGCATCCTTGCTACAGAAGTCACGCCGGAGGATG +CACAGAAGCTGTTGGTGGGGCTGAACCAGAGAATAGTGGTTAACGGCAGAACGCAACGGA +ATACGAACACCATGAAAAACTATCTGCTTCCCGTGGTCGCCCAAGCCTTCAGTAAGTGGG +CAAAGGAGTGCCGGAAAGACATGGAAGATGAAAAACTCCTGGGGGTCAGAGAAAGAACAC +TGACCTGCTGCTGTCTATGGGCATTCAAGAAGCAGAAAACACACACGGTCTACAAGAGGC +CTGATACCCAGTCAATTCAGAAGGTTCAGGCCGAGTTTGACAGCTTTGTGGTACCGAGCC +TGTGGTCGTCCGGGTTGTCAATCCCCTTGAGGACTAGAATCAAATGGTTGTTAAGCAAGG +TGCCAAAAACCGACCTGATCCCATATAGCGGGGACGCCCAAGAAGCCCGGGACGCAGAAA +AAGAAGCAGAGGAAGAACGAGAAGCAGAACTGACTCGTGAAGCCCTACCACCTCTACAGG +CAGCACAGGAAGATGTTCAGGTCGAAATCGACGTGGAACAGCTTGAGGACAGAGCGGGTG +CAGGAATAATAGAGACTCCTAGAGGAGCTATCAAAGTTACTGCCCAACCAACAGACCACG +TCGTGGGAGAGTACCTGGTACTTTCCCCGCAGACCGTACTACGGAGCCAGAAGCTCAGCC +TAATTCACGCTTTGGCGGAGCAAGTGAAGACGTGCACGCACAGCGGACGAGCAGGGAGGT +ACGCGGTCGAAGCGTACGACGGCCGAGTCCTAGTGCCCTCAGGCTATGCAATCTCGCCTG +AAGACTTCCAGAGTCTAAGCGAAAGCGCAACGATGGTGTATAACGAAAGAGAGTTCGTAA +ACAGAAAGCTACACCACATTGCGATGCACGGACCAGCCCTGAACACCGACGAAGAGTCGT +ATGAGCTGGTGAGGGCAGAGAGGACAGAACACGAGTACGTCTACGATGTGGACCAGAGAA +GATGCTGTAAGAAGGAAGAAGCTGCAGGACTGGTACTGGTGGGCGACTTGACTAATCCGC +CCTACCACGAATTCGCATATGAAGGGCTAAAAATCCGCCCTGCCTGCCCATACAAAATTG +CAGTCATAGGAGTCTTCGGAGTACCAGGATCTGGCAAGTCAGCTATTATCAAGAACCTAG +TTACCAGGCAAGACCTGGTGACTAGCGGAAAGAAAGAAAACTGCCAAGAAATCACCACCG +ACGTGATGAGACAGAGAGGTCTAGAGATATCTGCACGTACGGTTGACTCGCTGCTCTTGA +ATGGATGCAACAGGCCAGTCGACGTGTTGTACGTAGACGAGGCGTTTGCGTGCCACTCTG +GAACGTTACTTGCTTTGATCGCCTTGGTTAGACCAAGGCAGAAAGTTGTACTCTGTGGTG +ACCCGAAGCAGTGCGGCTTCTTCAATATGATGCAGATGAAAGTCAACTATAATCACAACA +TCTGCACCCAAGTGTACCACAAAAGTATCTCCAGGCGGTGTACACTGCCTGTGACCGCCA +TTGTGTCATCGTTGCATTACGAAGGCAAAATGCGCACTACGAATGAGTACAACAAGCCGA +TTGTAGTGGACACTACAGGCTCAACAAAACCTGACCCTGGAGATCTCGTGTTAACGTGCT +TCAGAGGGTGGGTTAAACAACTGCAAATTGACTATCGTGGATACGAGGTCATGACAGCAG +CCGCATCCCAAGGGTTAACCAGAAAAGGAGTTTATGCAGTTAGGCAAAAAGTTAATGAAA +ACCCGCTCTATGCATCAACGTCAGAGCACGTCAACGTACTCCTAACACGTACGGAAGGTA +AACTGGTATGGAAGACACTTTCCGGTGACCCGTGGATAAAGACGCTGCAGAACCCACCGA +AAGGAAACTTCAAAGCAACTATCAAGGAGTGGGAGGTGGAGCATGCATCAATAATGGCGG +GCATCTGCAGTCACCAAATGACCTTTGATACATTCCAAAACAAAGCCAATGTTTGTTGGG +CTAAGAGCTTGGTCCCTATCCTCGAAACAGCGGGGATAAAACTAAATGATAGGCAGTGGT +CCCAGATAATTCAAGCCTTCAAAGAAGATAAAGCATACTCACCTGAAGTAGCCCTGAATG +AAATATGCACGCGCATGTATGGGGTGGATTTAGACAGTGGGCTATTTTCTAAACCATTGG +TGTCTGTGTATTACGCGGATAACCACTGGGATAATAGGCCTGGAGGGAAGATGTTCGGAT +TCAACCCCGAGGCAGCATCCATTCTAGAAAGAAAGTATCCATTCACAAAAGGGAAGTGGA +ACATCAACAAGCAGATCTGCGTGACCACCAGGAGGATAGAAGACTTTAACCCTACCACCA +ATATTATACCGGCCAATAGGAGACTACCACACTCATTAGTGGCCGAACACCGCCCAGTAA +AAGGGGAAAGAATGGAATGGCTGGTTAATAAGATAAACGGCCACCACGTGCTCCTGGTCA +GTGGCTATAATCTTGTACTGCCTACTAAGAGAGTCACTTGGGTAGCGCCACTAGGTGTCC +GCGGAGCGGACTACACATACAACCTAGAGTTGGGTCTGCCAGCAACGCTTGGCAGGTATG +ACCTAGTGGTCATAAACATCCACACACCTTTTCGCATACACCATTACCAACAGTGCGTAG +ACCACGCAATGAAACTGCAAATGCTCGGGGGTGACTCATTGAGACTGCTCAAACCGGGTG +GCTCTCTATTGATCAGAGCATATGGTTACGCAGATAGAACCAGTGAACGAGTCATCTGCG +TGTTGGGACGCAAGTTCAGATCGTCTAGAGCGTTGAAACCACCATGTGTCACCAGCAACA +CTGAGATGTTTTTCCTATTCAGCAACTTTGACAATGGCAGAAGGAATTTCACAACTCATG +TCATGAATAATCAACTGAATGCAGCCTTTGTAGGACAGGCCACCCGAGCAGGATGTGCAC +CGTCGTACCGGGTAAAACGCATGGACATCGCGAAGAACGATGAAGAGTGCGTAGTTAACG +CCGCTAACCCTCGCGGGTTACCGGGTGACGGTGTTTGCAAGGCAGTATACAAAAAATGGC +CGGAGTCCTTTAAGAATAGTGCAACACCAGTGGGAACCGCAAAAACAGTCATGTGCGGTA +CGTATCCAGTAATCCACGCCGTTGGACCGAACTTCTCTAATTATTTGGAATCTGAAGGGG +ACCGGGAATTGGCAGCTGCCTATCGAGAAGTCGCAAAGGAAGTAACTAGACTGGGAGTAA +ATAGTGTAGCTATACCTCTCCTCTCCACAGGTGTATACTCAGGAGGGAAAGATAGGCTGA +CCCAGTCACTGAACCACCTCTTCACAGCCATGGACTCGACGGATGCAGACGTGGTCATCT +ACTGTCGCGACAAAGAATGGGAGAAGAAAATATCCGAGGCCATACAGATGCGGACTCAAG +TGGAGTTGCTGGATGAGCACATCTCCATAGACTGCGATATTGTTCGCGTGCACCCTGACA +GCAGCTTGGCAGGCAGAAAAGGATACAGCACCACGGAAGGCGCACTGTACTCATATCTAG +AAGGGACTCGTTTTCACCAGACGGCAGTGGACATGGCAGAGATATATACTATGTGGCCAA +AGCAAACAGAGGCCAACGAGCAAGTCTGCCTATATGCCCTGGGGGAAAGTATTGAATCGA +TCAGGCAGAAATGCCCGGTGGATGATGCAGATGCATCGTCTCCTCCAAAAACTGTCCCGT +GCCTTTGCCGTTACGCCATGACTCCGGAACGCGTCACCCGACTTCGCATGAACCACGTCA +CAAGTATAATTGTGTGTTCCTCGTTTCCCCTTCCAAAGTACAAAATAGAAGGAGTGCAAA +AAGTCAAATGCTCTAAGGTAATGCTATTTGACCACAACGTGCCATCACGCGTAAGTCCAA +GGGAATATAGATCTTCCCCGGAGGCTGCACAGGAAGCGAGTACAACCACGTCACTGACGC +ATAGCCAATTCGACCTAAGCGTTGACGGCGAGATACTACCCGTCCCGTCAGATCTGGATG +CTGACGCCCCAGCCCTAGAACCAGCTCTAGACGACAGGGCGACACACACGTTGCCATCCA +CAACCGGAAACCTTGCGGCCGTGTCTGACTGGGTAATGAGCACCGTACCTGTCGCGCCGC +CCAGAAGAAGGCGAGGGAGAAACCTGACTGTGACATGCGACGAGAGAGAAGGGAACATAA +CACCCATGGCTAGCGTCCGATTCTTTAGGGCAGAGCTGCGTCCGGTCGTACAAGAAACAG +CGGAGACGCGTGACACAGCAATGTCTCTTCAGGCACCGCCGAGTACCGCCACGGAACTGA +ATCATCCGCCGATCTCCTTCGGAGCACCAAGCGAGACGTTCCCCATTACATTTGGGGACT +TCAACGAAGGAGAAATCGAAAGCTTGTCTTCTGAGCTGCTAACTTTCGGAGACTTCCTAC +CCGGAGAAGTGGATGACTTGACAGATAGCGACTGGTCCACGTGCTCAGACACGGACGACG +AGTTATGACTAGACAGGGCAGGTGGGTATATATTCTCGTCGGACACCGGTCCAGGTCATT +TACAACAGAAGTCAGTACGCCAGTCAGTGCTGCCGGTGAACACCCTGGAGGAAGTCCACG +AGGAGAAGTGTTACCCACCTAAGTTGGATGAAGTAAAGGAGCAACTATTACTTAAGAAAC +TCCAGGAGAGTGCATCCATGGCCAACAGAAGCAGGTACCAGTCGCGCAAAGTAGAAAACA +TGAAAGCAACAATCATCCAGAGACTAAAGAGAGGCTGTAGACTATACTTAATGTCAGAGA +CCTCAAAAGTCCCTACTTACCGGACCACATATCCGGCGCCTGTGTACTCGCCTCCGATCA +ACGTCCGACTGTCCAACCCCGAGTCCGCAGTGGCAGCATGCAATGAGTTCCTAGCTAGAA +ACTATCCAACTGTCTCATCATACCAAATTACCGACGAGTATGATGCATATTTAGACATGG +TGGACGGGTCGGAGAGTTGCCTGGATCGAGCGACATTCAATCCGTCAAAACTTAGGAGCT +ACCCGAAACAGCACGCTTACCATGCGCCCTCCATCAGAAGCGCTGTACCGTCCCCATTCC +AGAACACACTACAGAATGTACTGGCAGCAGCCACGAAAAGAAACTGCAACGTCACACAGA +TGAGGGAATTACCCACTTTGGACTCAGCAGTATTCAACGTGGAGTGTTTTAAAAAATTCG +CATGCAACCAAGAATACTGGGAAGAATTTGCTGCCAGCCCTATTAGGATAACAACTGAGA +ATTTAACAACCTATGTTACTAAACTAAAAGGGCCAAAAGCAGCAGCGCTATTTGCAAAAA +CCCACAATCTGCTGCCACTGCAGGAAGTACCAATGGATAGGTTCACAGTAGATATGAAAA +GGGACGTGAAGGTGACTCCTGGTACAAAGCACACAGAGGAAAGACCTAAGGTGCAGGTTA +TACAGGCGGCTGAACCCTTGGCGACAGCATACCTATGTGGGATTCACAGAGAGCTGGTTA +GGAGGCTGAACGCCGTCCTCCTACCCAATGTACATACACTATTTGATATGTCTGCCGAGG +ATTTCGATGCCATCATAGCCGCACACTTTAAGCCAGGAGACACTGTTTTAGAAACGGACA +TAGCCTCCTTTGACAAGAGCCAAGATGATTCACTTGCGCTTACTGCCTTGATGTTGTTAG +AAGATTTAGGGGTGGATCACTCCCTGTTGGACTTGATAGAGGCCGCTTTTGGAGAGATTT +CCAGCTGTCATCTACCGACAGGTACGCGCTTCAAGTTCGGCGCCATGATGAAATCTGGTA +TGTTCCTAACTCTGTTCGTCAACACATTGCTAAACATCACCATCGCTAGCCGCGTGCTGG +AAGATCGTCTGACAAAATCCGCGTGCGCGGCCTTCATCGGCGACGACAACATAATACATG +GAGTCGTCTCCGATGAATTGATGGCAGCCAGATGCGCCACTTGGATGAACATGGAAGTGA +AGATCATAGATGCAGTTGTATCCTTGAAAGCCCCTTACTTTTGTGGAGGGTTTATACTGC +ACGATACTGTGACGGGAACAGCCTGCAGAGTGGCAGACCCGCTAAAAAGGCTATTCAAAC +TGGGCAAACCGCTAGCGGCAGGTGACGAACAAGATGAAGATAGAAGACGAGCGCTGGCTG +ACGAAGTGATCAGATGGCAACGAACAGGGCTAATTGATGAGTTGGAGAAAGCGGTATACT +CTAGGTATGAAGTGCAGGGTATATCAGTTGTGGTAATGTCCATGGCCACCTTTGCAAGCT +CCAGATCTAACTTCGAGAAGCTCAGAGGACCCGTCATAACTTTGTACGGCGGTCCTAAAT +AGGTACGCACTACAGCTACCTATTTTGCAGAAGCCGACAGCAAGTACCTAAATACTAATT +AGCTACAATGGAGTACATCCCAACCCAAACTTTTTACAATAGGAGGTACCAGCCTCGACC +CTGGACTCCGCGCCCCACTATCCAAGTAATCAGGCCCAGACCGCGCCCTCAGAGGCAAGC +CGGGCAACTTGCCCAGCTGATCTCAGCAGTTAATAAACTGACAATGCGCGCGGTACCCCA +ACAGAAGCCACGCAGGAATCGGAAGAATAAGAAGCAAAAGCAAAAGCGACAGGCGCCACA +AAACAACACAAACCAAAAGAAGCAGCCGCCTAAAAAGAAACCAGCTCAAAAGAAAAAGAA +GCCGGGCCGCAGAGAGAGGATGTGCATGAAAATCGAAAATGATTGTATTTTTGAAGTCAA +GTATGAAGGTAAGGTAACAGGTTACGCGTGCTTGGTGGGGGACAAAGTAATGAAACCAGC +ACACGTAAAGGGGACCATCGATAACGCGGACCTGGCCAAACTGGCCTTTAAGCGGTCATC +TAAGTATGACCTTGAATGCGCGCAGATACCCGTGCACATGAAGTCCGACGCTTCGAAGTT +CACCCATGAGAAACCAGAGGGGTACTACAACTGGCACCACGGAGCAGTACAGTACTCAGG +AGGCCGGTTCACCATCCCCACAGGTGCTGGCAAACCAGGGGACAGCGGCAGACCGATCTT +CGACAACAAGGGACGCGTGGTGGCCATAGTCTTAGGAGGAGCTAATGAAGGAGCCCGTAC +AGCCCTCTCGGTGGTGACCTGGAATAAAGACATTGTCACTAAAATCACCCCCGAGGGGGC +CGAAGAGTGGAGTCTTGCCATCCCAGTCATGTGCCTGCTGGCAAATACCACGTTCCCCTG +CTCCCAGCCCCCTTGCACTCCCTGCTGCTATGAAAGGGAACCAGAGGAAACCCTACGCAT +GCTTGAGGACAACGTCATGAGACCTGGGTACTATCAGCTGCTACAAGCATCCTTAACATG +TTCTCCCCACCGTCAGCGACGCAGTACCAAGGACAACTTCAATGTCTATAAAGCCACAAG +ACCGTACTTAGCTCACTGTCCCGACTGTGGAGAAGGGCACTCGTGCCATAGTCCCGTAGC +ATTAGAACGCATCAGAAATGAAGCGACAGACGGGACGCTGAAAATCCAGGTCTCCTTGCA +AATCGGAATAAAGACGGATGATAGCCACGATTGGACCAAGCTGCGTTACATGGACAACCA +CACGCCAGCGGACGCAGAGAGGGCGGGGCTATTTGTAAGAACATCAGCACCGTGCACGAT +TACTGGAACAATGGGACACTTCATCCTGACCCGATGTCCGAAAGGGGAAACTCTGACGGT +GGGATTCACTGACAGTAGGAAGATCAGTCACTCATGTACGCACCCATTTCACCACGACCC +TCCTGTGATAGGCCGGGAGAAATTCCATTCCCGACCGCAGCACGGTAAAGAGCTGCCTTG +CAGCACGTACGTGCAGAGCACCGCCGCAACTACCGAGGAGATAGAGGTACACATGCCCCC +AGACACCCCTGATCGCACATTGATGTCACAACAGTCCGGCAACGTAAAGATCACAGTTAA +TGGCCAGACGGTGCGGTACAAGTGTAATTGCGGTGGCTCAAATGAAGGACTAATAACTAC +AGACAAAGTGATTAATAACTGCAAAGTTGATCAATGTCATGCCGCGGTCACCAATCACAA +AAAGTGGCAGTACAACTCCCCTCTGGTCCCGCGTAATGCTGAACTTGGGGACCGAAAAGG +AAAAATCCACATCCCGTTTCCGCTGGCAAATGTAACATGCAGGGTGCCTAAAGCAAGGAA +CCCCACCGTGACGTACGGGAAAAACCAAGTCATCATGCTACTGTATCCCGACCACCCAAC +ACTCCTGTCCTACCGGAACATGGGAGAAGAACCAAACTACCAAGAAGAGTGGGTGACGCA +TAAGAAGGAAGTCGTGCTAACCGTGCCGACTGAAGGGCTCGAGGTCACGTGGGGTAACAA +CGAGCCGTATAAGTATTGGCCGCAGTTATCTACAAACGGTACAGCCCATGGCCACCCGCA +TGAGATAATTCTGTATTATTATGAGCTGTACCCTACTATGACTGTAGTAGTTGTGTCAGT +GGCCTCGTTCGTACTCCTGTCGATGGTGGGTGTGGCAGTGGGGATGTGCATGTGTGCACG +ACGCAGATGCATCACACCGTACGAACTGACACCAGGAGCTACCGTCCCTTTCCTGCTTAG +CCTAATATGCTGCATCAGAACAGCTAAAGCGGCCACATACCAAGAGGCTGCGGTATACCT +GTGGAATGAGCAGCAACCTTTGTTTTGGCTACAAGCCCTTATTCCGCTGGCAGCCCTGAT +TGTCCTATGCAACTGTCTGAGACTCTTACCATGCTGCTGTAAAACGTTGGCTTTTTTAGC +CGTAATGAGCGTCGGTGCCCACACTGTGAGCGCGTACGAACACGTAACAGTGATCCCGAA +CACGGTGGGAGTACCGTATAAGACTCTAGTCAATAGACCGGGCTACAGTCCCATGGTATT +GGAGATGGAACTACTGTCAGTCACTTTGGAGCCAACGCTATCGCTTGATTACATCACGTG +CGAGTACAAAACCGTTATCCCGTCTCCGTACGTGAAATGCTGCGGTACAGCAGAGTGCAA +GGACAAAAACCTACCTGACTACAGCTGTAAGGTCTTCACCGGCGTCTACCCATTTATGTG +GGGCGGAGCCTACTGCTTCTGCGACGCTGAAAACACGCAATTGAGCGAAGCACACGTGGA +GAAGTCCGAATCATGCAAAACAGAATTTGCATCAGCATACAGGGCTCATACCGCATCCGC +ATCAGCTAAGCTCCGCGTCCTTTACCAAGGAAATAACATCACTGTAACTGCCTATGCTAA +CGGCGACCATGCCGTCACAGTTAAGGACGCCAAATTCATTGTGGGGCCAATGTCTTCAGC +CTGGACACCTTTCGACAACAAAATTGTGGTGTACAAAGGTGACGTCTATAACATGGACTA +CCCGCCCTTTGGCGCAGGAAGACCAGGACAATTTGGCGATATCCAAAGTCGCACACCTGA +GAGTAAAGACGTCTATGCTAATACACAACTGGTACTGCAGAGACCGGCTGCGGGTACGGT +ACATGTGCCATACTCTCAGGCACCATCTGGCTTTAAGTATTGGCTAAAAGAACGAGGGGC +GTCGCTGCAGCACACAGCACCATTTGGCTGCCAAATAGCAACAAACCCGGTAAGAGCGGT +GAATTGCGCCGTAGGGAACATGCCCATCTCCATCGACATACCGGATGCGGCCTTCATTAG +GGTCGTCGACGCGCCCTCTTTAACGGACATGTCGTGCGAGGTACCAGCCTGCACCCATTC +CTCAGATTTCGGGGGCGTCGCCATTATTAAATATGCAGCCAGCAAGAAAGGCAAGTGTGC +GGTGCATTCGATGACCAACGCCGTCACAATTCGGGAAGCTGAGATAGAAGTTGAAGGGAA +TTCTCAGCTGCAAATCTCTTTCTCGACGGCCTTGGCCAGCGCCGAATTCCGCGTACAAGT +CTGTTCTACACAAGTACACTGTGTAGCCGAGTGCCACCCTCCGAAGGACCACATAGTCAA +TTACCCGGCGTCACATACCACCCTCGGGGTCCAGGACATTTCCGCTACGGCGCTGTCATG +GGTGCAGAAGATCACGGGAGGCGTGGGACTGGTTGTCGCTGTTGCAGCACTGATTCTAAT +CGTGGTGCTATGCGTGTCGTTCAGCAGGCACTAACTTGACAACTAAGTATGAAGGTATAT +GTGTCCCCTAAGAGACACACTGTAAATAGCTAATAATCTATAGATCAAAGGGCTATACAA +CCCCTGAATAGTAACAAAATACAAAATCACTAAAAATTATAAAAACAGAAAAATACATAA +ATAGGTATACGTGTCCCCTAAGAGACACATTGTATGTAGGTGATAAGTATAGATCAAAGG +GCCGAACAACCCCTGAATAGTAACAAAACATGAAAATCAATAAAAATCATAAAATAGAAA +AACCATAAACAGAAGTAGTTCAAAGGGCTATACAACCCCTGAATAGTAACAAAACACAAA +ACTAATAAAAATTAAATGAATACCATAATTGGCAAACGGAAGAGATGTAGGTACTTAAGC +TTCCTAAAAGCAGCCGAACTCACTTTGAGATGTAGGCATAGCATACCGAACTCTTCCACG +ATTCTCCGAACCCACAGGGACGTAGGAGATGTTATTTTGTTTTTAATATTTC diff --git a/primerschemes/zebra-chikv-ecsa/300/v1.0.0/README.md b/primerschemes/zebra-chikv-ecsa/300/v1.0.0/README.md new file mode 100644 index 0000000..4c9dd29 --- /dev/null +++ b/primerschemes/zebra-chikv-ecsa/300/v1.0.0/README.md @@ -0,0 +1,57 @@ +# zibra-chikv-ecsa-300 300bp v1.0.0 + +[![Generic badge](https://img.shields.io/badge/STATUS-draft-blue.svg)](https://labs.primalscheme.com/faqs) + +> If you use this scheme please cite: Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples. doi:10.1038/nprot.2017.066 + +[primalscheme labs](https://labs.primalscheme.com/detail/zibra-chikv-ecsa-300/300/v1.0.0) + +## Overviews + +## Details + +```json +{ + "ampliconsize": 300, + "schemeversion": "v1.0.0", + "schemename": "zibra-chikv-ecsa-300", + "primer_bed_md5": "69837908bf4d89963193fc2c673fcea5", + "reference_fasta_md5": "7f06dfa1bf98cd8bc7ed4b86ec000b40", + "status": "draft", + "citations": [ + "Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples. doi:10.1038/nprot.2017.066" + ], + "authors": [ + "quick-lab", + "loman-lab" + ], + "algorithmversion": "ps:100", + "species": [ + 37124 + ], + "license": "CC BY-SA 4.0", + "primerclass": "primerschemes", + "infoschema": "v2.1.0", + "articbedversion": "v3.0", + "collections": [], + "links": { + "protocols": [], + "validation": [], + "homepage": [], + "vendors": [], + "misc": [] + }, + "refselect": null, + "description": null, + "derivedfrom": null, + "contactinfo": null +} +``` + + + +------------------------------------------------------------------------ + +This work is licensed under a [Creative Commons Attribution-ShareAlike 4.0 International License](http://creativecommons.org/licenses/by-sa/4.0/) + +![](https://i.creativecommons.org/l/by-sa/4.0/88x31.png) \ No newline at end of file diff --git a/primerschemes/zebra-chikv-ecsa/300/v1.0.0/info.json b/primerschemes/zebra-chikv-ecsa/300/v1.0.0/info.json new file mode 100644 index 0000000..c82c464 --- /dev/null +++ b/primerschemes/zebra-chikv-ecsa/300/v1.0.0/info.json @@ -0,0 +1,35 @@ +{ + "ampliconsize": 300, + "schemeversion": "v1.0.0", + "schemename": "zibra-chikv-ecsa", + "primer_bed_md5": "69837908bf4d89963193fc2c673fcea5", + "reference_fasta_md5": "7f06dfa1bf98cd8bc7ed4b86ec000b40", + "status": "validated", + "citations": [ + "Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples. doi:10.1038/nprot.2017.066" + ], + "authors": [ + "quick-lab", + "loman-lab" + ], + "algorithmversion": "ps:100", + "species": [ + 37124 + ], + "license": "CC BY-SA 4.0", + "primerclass": "primerschemes", + "infoschema": "v2.1.0", + "articbedversion": "v3.0", + "collections": [], + "links": { + "protocols": [], + "validation": [], + "homepage": [], + "vendors": [], + "misc": [] + }, + "refselect": null, + "description": null, + "derivedfrom": null, + "contactinfo": null +} \ No newline at end of file diff --git a/primerschemes/zebra-chikv-ecsa/300/v1.0.0/primer.bed b/primerschemes/zebra-chikv-ecsa/300/v1.0.0/primer.bed new file mode 100644 index 0000000..ea93489 --- /dev/null +++ b/primerschemes/zebra-chikv-ecsa/300/v1.0.0/primer.bed @@ -0,0 +1,89 @@ +# artic-bed-version v3.0 +KP164568.1 115 137 CHIK400_1_LEFT_3 1 + CTTTTTGAAGGCCCTGCAACGT +KP164568.1 428 451 CHIK400_1_RIGHT_3 1 - ACGGCCATCACCTCTTGTAAGT +KP164568.1 294 316 CHIK400_2_LEFT_0 2 + TGTCGGACAGGAAGTACCACTG +KP164568.1 660 685 CHIK400_2_RIGHT_0 2 - CTCATCCGCCCAATTTGTCGAG +KP164568.1 595 617 CHIK400_3_LEFT_0 1 + GTACTGGGTAGGGTTCGACACA +KP164568.1 902 924 CHIK400_3_RIGHT_0 1 - CACACGAAACCACTGTGTCACA +KP164568.1 860 882 CHIK400_4_LEFT_4 2 + TCGGTGTTCCATCTAAAGGGCA +KP164568.1 1195 1217 CHIK400_4_RIGHT_4 2 - CTTCATGGTGTTCGTGTTCCGT +KP164568.1 1153 1175 CHIK400_5_LEFT_0 1 + GGTGGGGCTGAACCAGAGAATA +KP164568.1 1408 1430 CHIK400_5_RIGHT_0 1 - TACAAAGCTGTCGAATTCGGCC +KP164568.1 1349 1371 CHIK400_6_LEFT_4 2 + AAGCAGAAAACACACACGGTCT +KP164568.1 1686 1710 CHIK400_6_RIGHT_4 2 - GCGCCTCTCGGAGTCTCTATTA +KP164568.1 1614 1636 CHIK400_7_LEFT_0 1 + TACAGGCAGCACAGGAAGATGT +KP164568.1 1908 1930 CHIK400_7_RIGHT_0 1 - TGGAAGTCTTCGGGCGAAATTG +KP164568.1 1816 1838 CHIK400_8_LEFT_1 2 + GGAGCAAGTGAAGACGTGTACG +KP164568.1 2192 2214 CHIK400_8_RIGHT_1 2 - TATATGGACAAGCGGGGCGAAT +KP164568.1 2105 2127 CHIK400_9_LEFT_0 1 + TGCAAGAAGGAAGAAGCTGCAG +KP164568.1 2463 2485 CHIK400_9_RIGHT_0 1 - AAGGCGATCAAGGCAAGTAACG +KP164568.1 2368 2390 CHIK400_10_LEFT_0 2 + ATCTGCACGTACGGTTGATTCG +KP164568.1 2709 2731 CHIK400_10_RIGHT_0 2 - GGTTTTGTTGAGCCCGTAGTGT +KP164568.1 2663 2685 CHIK400_11_LEFT_0 1 + GGCAAAATGCGCACTACGAATG +KP164568.1 2916 2938 CHIK400_11_RIGHT_0 1 - CCTTCCGTACGCGTTAGGAGTA +KP164568.1 2845 2868 CHIK400_12_LEFT_1 2 + AGGAGTTTACGCAGTTAGGCAAA +KP164568.1 3220 3242 CHIK400_12_RIGHT_1 2 - TTCATTCAGGGCTACTTCGGGT +KP164568.1 3129 3151 CHIK400_13_LEFT_0 1 + TGGTCCCTATCCTCGAAACAGC +KP164568.1 3431 3453 CHIK400_13_RIGHT_0 1 - TCCTGGTAGTCACGCAGATCTG +KP164568.1 3367 3389 CHIK400_14_LEFT_0 2 + TGAGGCAGCGTCCATTCTAGAA +KP164568.1 3697 3719 CHIK400_14_RIGHT_0 2 - ATACCTACCAAGTGTTGCCGGT +KP164568.1 3609 3632 CHIK400_15_LEFT_4 1 + ACCTTGCACTGCCTACTAAGAGA +KP164568.1 3921 3947 CHIK400_15_RIGHT_4 1 - ACATGGTGGTTTCAATGCTCTGG +KP164568.1 3852 3874 CHIK400_16_LEFT_0 2 + TCAGAGCATACGGTTACGCAGA +KP164568.1 4221 4243 CHIK400_16_RIGHT_0 2 - TTTGCGGTTCCTACTGGTGTTG +KP164568.1 4166 4188 CHIK400_17_LEFT_0 1 + GACGGTGTTTGCAAGGCAGTAT +KP164568.1 4452 4474 CHIK400_17_RIGHT_0 1 - TCCATGGCTGTAAAGAGGTGGT +KP164568.1 4393 4415 CHIK400_18_LEFT_0 2 + ACCTCTCCTCTCCACAGGTGTA +KP164568.1 4731 4753 CHIK400_18_RIGHT_0 2 - GCCTCTATTTGCTTTGGCCACA +KP164568.1 4680 4702 CHIK400_19_LEFT_0 1 + AAGGGACACGTTTTCACCAGAC +KP164568.1 4970 4994 CHIK400_19_RIGHT_0 1 - GGAGCATTTGACTTTTTGCACTCC +KP164568.1 4898 4920 CHIK400_20_LEFT_0 2 + CGACTTCGCATGAACCATGTCA +KP164568.1 5187 5209 CHIK400_20_RIGHT_0 2 - GTATGTATCGCCCCGTCGTCTA +KP164568.1 4301 4332 CHIK400_21_LEFT_0 1 + AGTTTGATCTAAGCGCCGATGG +KP164568.1 5491 5513 CHIK400_21_RIGHT_0 1 - AAATGTGATGGGGAACGTCTCG +KP164568.1 5406 5429 CHIK400_22_LEFT_0 2 + CGCGTGACACAGCTATTTCCTT +KP164568.1 5700 5722 CHIK400_22_RIGHT_0 2 - TGGCGTACCGACTTCTGTTGTA +KP164568.1 5628 5650 CHIK400_23_LEFT_0 1 + ACACGGACGACGAGTTATGACT +KP164568.1 5991 6013 CHIK400_23_RIGHT_0 1 - GACAATCGGACGTTGATCGGAG +KP164568.1 5928 5950 CHIK400_24_LEFT_0 2 + TAATGGCAGAGACCCCGAAAGT +KP164568.1 6226 6248 CHIK400_24_RIGHT_0 2 - TGTGTTCTGGAATGGGGAAGGT +KP164568.1 6146 6168 CHIK400_25_LEFT_0 1 + CGAGCGACATTCAATCCGTCAA +KP164568.1 6475 6497 CHIK400_25_RIGHT_0 1 - CGGCAGCAGATTATGGGTTCTT +KP164568.1 6380 6402 CHIK400_26_LEFT_3 2 + GAAGAATTTGCTGCCAGCCCTA +KP164568.1 6722 6744 CHIK400_26_RIGHT_3 2 - GTGCGGCTATAATGGCATCGAA +KP164568.1 6657 6679 CHIK400_27_LEFT_0 1 + TTAGGAGATTGAACGCCGTCCT +KP164568.1 7008 7033 CHIK400_27_RIGHT_0 1 - GTCAGACGATCTTCCAACACCC +KP164568.1 6916 6938 CHIK400_28_LEFT_2 2 + GACAGGTACGCGCTTCAAGTTC +KP164568.1 7254 7277 CHIK400_28_RIGHT_2 2 - CGCTAACGGTTTGCCCAGTTTA +KP164568.1 7168 7191 CHIK400_29_LEFT_0 1 + AGCTCCCTACTTTTGTGGAGGG +KP164568.1 7472 7494 CHIK400_29_RIGHT_0 1 - GACCGCCGTACAAGGTTATGAC +KP164568.1 7413 7435 CHIK400_30_LEFT_0 2 + TAATGTCCATGGCCACCTTTGC +KP164568.1 7747 7769 CHIK400_30_RIGHT_0 2 - TATTCTTCCGATTCTTGCGCGG +KP164568.1 7684 7706 CHIK400_31_LEFT_0 1 + CAACTTGCCCAGCTGATCTCAG +KP164568.1 7980 8002 CHIK400_31_RIGHT_0 1 - ATCGATGGTCCCCTTTACGTGT +KP164568.1 7929 7951 CHIK400_32_LEFT_0 2 + TAAGGTAACAGGTTACGCGTGC +KP164568.1 8257 8279 CHIK400_32_RIGHT_0 2 - TACGGGCTCCTTCATTAGCTCC +KP164568.1 8210 8232 CHIK400_33_LEFT_2 1 + GACCGATCTTCGACAACAAGGG +KP164568.1 8510 8532 CHIK400_33_RIGHT_2 1 - GCGTCGGGGAGAACATGTTAAG +KP164568.1 8441 8465 CHIK400_34_LEFT_0 2 + GAGAAAACCTTGCGCATGCTTG +KP164568.1 8749 8771 CHIK400_34_RIGHT_0 2 - CTGCTGGCATGTGATTGTCCAT +KP164568.1 8685 8707 CHIK400_35_LEFT_0 1 + CCAGGTTTCCTTGCAAATCGGA +KP164568.1 8983 9010 CHIK400_35_RIGHT_0 1 - GTACGTGCTGCAAGGTAGTTCC +KP164568.1 8912 8934 CHIK400_36_LEFT_1 2 + CATGTACGCACCCATTTCACCA +KP164568.1 9270 9293 CHIK400_36_RIGHT_1 2 - GGTCCCCGAATTCAGCATTACG +KP164568.1 9198 9220 CHIK400_37_LEFT_0 1 + CTGCAAGGTCGATCAATGCCAT +KP164568.1 9521 9543 CHIK400_37_RIGHT_0 1 - TCATTGTTACCCCACGTGACCT +KP164568.1 9467 9489 CHIK400_38_LEFT_0 2 + AGTGGGTGACGCATAAGAAGGA +KP164568.1 9731 9754 CHIK400_38_RIGHT_0 2 - TGGTGTCAGTTCGTACGGTGTA +KP164568.1 9670 9692 CHIK400_39_LEFT_0 1 + ATACTCCTGTCGATGGTGGGTG +KP164568.1 9996 10018 CHIK400_39_RIGHT_0 1 - CGGGATCACTGTTACGTGTTCG +KP164568.1 9952 9974 CHIK400_40_LEFT_0 2 + TTTTTAGCCGTACTGAGCGTCG +KP164568.1 10272 10294 CHIK400_40_RIGHT_0 2 - ATTTTCGGTGTCGCAGAAGCAG +KP164568.1 10181 10203 CHIK400_41_LEFT_0 1 + GCGGTACAGCAGAGTGTAAGGA +KP164568.1 10520 10542 CHIK400_41_RIGHT_0 1 - GTCGCCTTTGTACACCACGATT +KP164568.1 10446 10468 CHIK400_42_LEFT_0 2 + CCATGCCGTCACAGTTAAGGAC +KP164568.1 10782 10804 CHIK400_42_RIGHT_0 2 - AGTTCATCGCTCTTACCGGGTT +KP164568.1 10717 10739 CHIK400_43_LEFT_0 1 + ATTGGCTAAAAGAACGAGGGGC +KP164568.1 10994 11016 CHIK400_43_RIGHT_0 1 - TTCCCGAATAGTGACGGCGTTA +KP164568.1 10909 10931 CHIK400_44_LEFT_0 2 + GCACCCATTCCTCAGACTTTGG +KP164568.1 11285 11307 CHIK400_44_RIGHT_0 2 - CCTGCTAAACGACACGCATAGC diff --git a/primerschemes/zebra-chikv-ecsa/300/v1.0.0/reference.fasta b/primerschemes/zebra-chikv-ecsa/300/v1.0.0/reference.fasta new file mode 100644 index 0000000..7ce678c --- /dev/null +++ b/primerschemes/zebra-chikv-ecsa/300/v1.0.0/reference.fasta @@ -0,0 +1,198 @@ +>KP164568.1 Chikungunya virus isolate BHI3734/H804698, complete genome +GTAGGCTACGTGTGACACACGTAGCCTACCAGTTTCTTACTGCTCTACTCTGCAAAGCAA +GAGATTAAGAACCCATCATGGATCCTGTGTACGTGGACATAGACGCTGACAGCGCCTTTT +TGAAGGCCCTGCAACGTGCGTACCCCATGTTTGAGGTGGAACCCAGGCAGGTCACACCGA +ATGACCATGCTAATGCTAGAGCGTTCTCGCATCTAGCTATAAAACTAATAGAGCAGGAAA +TTGATCCCGACTCAACCATCCTGGACATCGGCAGTGCACCAGCAAGGAGGATGATGTCGG +ACAGGAAGTACCACTGCGTCTGCCCGATGCGCAGCGCAGAAGATCCCGAGAGACTCGCCA +ATTATGCGAGAAAGCTAGCATCTGCTGCAGGAAAAGTCCTGGACAGAAACATCTCTGGAA +AGATCGGGGACTTACAAGCAGTAATGGCCGTGCCAGACACGGAGACGCCAACATTCTGCT +TGCACACAGATGTCTCATGTAGACAGAGAGCAGACGTCGCGATATACCAAGACGTCTATG +CTGTACACGCACCCACGTCGCTATACCACCAGGCGATTAAAGGGGTCCGAGTGGCGTACT +GGGTAGGGTTCGACACAACCCCGTTCATGTACAATGCCATGGCGGGTGCCTACCCCTCAT +ATTCGACAAACTGGGCAGACGAGCAGGTACTGAAGGCTAAGAACATAGGATTATGTTCAA +CAGACCTGACGGAAGGTAGACGAGGTAAATTGTCTATTATGAGAGGGAAGAAGCTAAAAC +CGTGTGACCGTGTGCTGTTCTCAGTAGGGTCAACGCTCTACCCGGAAAGCCGCAAGCTAC +TTAAGAGCTGGCACTTACCATCGGTGTTCCATCTAAAGGGCAAACTCAGCTTCACATGCC +GCTGTGATACAGTGGTTTCGTGCGAGGGCTACGTCGTTAAGAGAATAACGATGAGCCCAG +GCCTTTATGGAAAAACCACAGGGTATGCGGTAACCCACCACGCAGACGGATTCTTGATGT +GCAAGACTACCGACACGGTTGACGGCGAAAGAGTGTCATTCTCGGTGTGCACATACGTGC +CGGCGACCATTTGTGATCAAATGACCGGCATCCTTGCTACAGAAGTCACGCCGGAGGATG +CACAGAAGCTGTTGGTGGGGCTGAACCAGAGAATAGTGGTTAACGGCAGAACGCAACGGA +ATACGAACACCATGAAAAACTATCTGCTTCCCGTGGTCGCCCAAGCCTTCAGTAAGTGGG +CAAAGGAGTGCCGGAAAGACATGGAAGATGAAAAACTCCTGGGGGTCAGAGAAAGAACAC +TGACCTGCTGCTGTCTATGGGCATTCAAGAAGCAGAAAACACACACGGTCTACAAGAGGC +CTGATACCCAGTCAATTCAGAAGGTTCAGGCCGAGTTTGACAGCTTTGTGGTACCGAGCC +TGTGGTCGTCCGGGTTGTCAATCCCCTTGAGGACTAGAATCAAATGGTTGTTAAGCAAGG +TGCCAAAAACCGACCTGATCCCATATAGCGGGGACGCCCAAGAAGCCCGGGACGCAGAAA +AAGAAGCAGAGGAAGAACGAGAAGCAGAACTGACTCGTGAAGCCCTACCACCTCTACAGG +CAGCACAGGAAGATGTTCAGGTCGAAATCGACGTGGAACAGCTTGAGGACAGAGCGGGTG +CAGGAATAATAGAGACTCCTAGAGGAGCTATCAAAGTTACTGCCCAACCAACAGACCACG +TCGTGGGAGAGTACCTGGTACTTTCCCCGCAGACCGTACTACGGAGCCAGAAGCTCAGCC +TAATTCACGCTTTGGCGGAGCAAGTGAAGACGTGCACGCACAGCGGACGAGCAGGGAGGT +ACGCGGTCGAAGCGTACGACGGCCGAGTCCTAGTGCCCTCAGGCTATGCAATCTCGCCTG +AAGACTTCCAGAGTCTAAGCGAAAGCGCAACGATGGTGTATAACGAAAGAGAGTTCGTAA +ACAGAAAGCTACACCACATTGCGATGCACGGACCAGCCCTGAACACCGACGAAGAGTCGT +ATGAGCTGGTGAGGGCAGAGAGGACAGAACACGAGTACGTCTACGATGTGGACCAGAGAA +GATGCTGTAAGAAGGAAGAAGCTGCAGGACTGGTACTGGTGGGCGACTTGACTAATCCGC +CCTACCACGAATTCGCATATGAAGGGCTAAAAATCCGCCCTGCCTGCCCATACAAAATTG +CAGTCATAGGAGTCTTCGGAGTACCAGGATCTGGCAAGTCAGCTATTATCAAGAACCTAG +TTACCAGGCAAGACCTGGTGACTAGCGGAAAGAAAGAAAACTGCCAAGAAATCACCACCG +ACGTGATGAGACAGAGAGGTCTAGAGATATCTGCACGTACGGTTGACTCGCTGCTCTTGA +ATGGATGCAACAGGCCAGTCGACGTGTTGTACGTAGACGAGGCGTTTGCGTGCCACTCTG +GAACGTTACTTGCTTTGATCGCCTTGGTTAGACCAAGGCAGAAAGTTGTACTCTGTGGTG +ACCCGAAGCAGTGCGGCTTCTTCAATATGATGCAGATGAAAGTCAACTATAATCACAACA +TCTGCACCCAAGTGTACCACAAAAGTATCTCCAGGCGGTGTACACTGCCTGTGACCGCCA +TTGTGTCATCGTTGCATTACGAAGGCAAAATGCGCACTACGAATGAGTACAACAAGCCGA +TTGTAGTGGACACTACAGGCTCAACAAAACCTGACCCTGGAGATCTCGTGTTAACGTGCT +TCAGAGGGTGGGTTAAACAACTGCAAATTGACTATCGTGGATACGAGGTCATGACAGCAG +CCGCATCCCAAGGGTTAACCAGAAAAGGAGTTTATGCAGTTAGGCAAAAAGTTAATGAAA +ACCCGCTCTATGCATCAACGTCAGAGCACGTCAACGTACTCCTAACACGTACGGAAGGTA +AACTGGTATGGAAGACACTTTCCGGTGACCCGTGGATAAAGACGCTGCAGAACCCACCGA +AAGGAAACTTCAAAGCAACTATCAAGGAGTGGGAGGTGGAGCATGCATCAATAATGGCGG +GCATCTGCAGTCACCAAATGACCTTTGATACATTCCAAAACAAAGCCAATGTTTGTTGGG +CTAAGAGCTTGGTCCCTATCCTCGAAACAGCGGGGATAAAACTAAATGATAGGCAGTGGT +CCCAGATAATTCAAGCCTTCAAAGAAGATAAAGCATACTCACCTGAAGTAGCCCTGAATG +AAATATGCACGCGCATGTATGGGGTGGATTTAGACAGTGGGCTATTTTCTAAACCATTGG +TGTCTGTGTATTACGCGGATAACCACTGGGATAATAGGCCTGGAGGGAAGATGTTCGGAT +TCAACCCCGAGGCAGCATCCATTCTAGAAAGAAAGTATCCATTCACAAAAGGGAAGTGGA +ACATCAACAAGCAGATCTGCGTGACCACCAGGAGGATAGAAGACTTTAACCCTACCACCA +ATATTATACCGGCCAATAGGAGACTACCACACTCATTAGTGGCCGAACACCGCCCAGTAA +AAGGGGAAAGAATGGAATGGCTGGTTAATAAGATAAACGGCCACCACGTGCTCCTGGTCA +GTGGCTATAATCTTGTACTGCCTACTAAGAGAGTCACTTGGGTAGCGCCACTAGGTGTCC +GCGGAGCGGACTACACATACAACCTAGAGTTGGGTCTGCCAGCAACGCTTGGCAGGTATG +ACCTAGTGGTCATAAACATCCACACACCTTTTCGCATACACCATTACCAACAGTGCGTAG +ACCACGCAATGAAACTGCAAATGCTCGGGGGTGACTCATTGAGACTGCTCAAACCGGGTG +GCTCTCTATTGATCAGAGCATATGGTTACGCAGATAGAACCAGTGAACGAGTCATCTGCG +TGTTGGGACGCAAGTTCAGATCGTCTAGAGCGTTGAAACCACCATGTGTCACCAGCAACA +CTGAGATGTTTTTCCTATTCAGCAACTTTGACAATGGCAGAAGGAATTTCACAACTCATG +TCATGAATAATCAACTGAATGCAGCCTTTGTAGGACAGGCCACCCGAGCAGGATGTGCAC +CGTCGTACCGGGTAAAACGCATGGACATCGCGAAGAACGATGAAGAGTGCGTAGTTAACG +CCGCTAACCCTCGCGGGTTACCGGGTGACGGTGTTTGCAAGGCAGTATACAAAAAATGGC +CGGAGTCCTTTAAGAATAGTGCAACACCAGTGGGAACCGCAAAAACAGTCATGTGCGGTA +CGTATCCAGTAATCCACGCCGTTGGACCGAACTTCTCTAATTATTTGGAATCTGAAGGGG +ACCGGGAATTGGCAGCTGCCTATCGAGAAGTCGCAAAGGAAGTAACTAGACTGGGAGTAA +ATAGTGTAGCTATACCTCTCCTCTCCACAGGTGTATACTCAGGAGGGAAAGATAGGCTGA +CCCAGTCACTGAACCACCTCTTCACAGCCATGGACTCGACGGATGCAGACGTGGTCATCT +ACTGTCGCGACAAAGAATGGGAGAAGAAAATATCCGAGGCCATACAGATGCGGACTCAAG +TGGAGTTGCTGGATGAGCACATCTCCATAGACTGCGATATTGTTCGCGTGCACCCTGACA +GCAGCTTGGCAGGCAGAAAAGGATACAGCACCACGGAAGGCGCACTGTACTCATATCTAG +AAGGGACTCGTTTTCACCAGACGGCAGTGGACATGGCAGAGATATATACTATGTGGCCAA +AGCAAACAGAGGCCAACGAGCAAGTCTGCCTATATGCCCTGGGGGAAAGTATTGAATCGA +TCAGGCAGAAATGCCCGGTGGATGATGCAGATGCATCGTCTCCTCCAAAAACTGTCCCGT +GCCTTTGCCGTTACGCCATGACTCCGGAACGCGTCACCCGACTTCGCATGAACCACGTCA +CAAGTATAATTGTGTGTTCCTCGTTTCCCCTTCCAAAGTACAAAATAGAAGGAGTGCAAA +AAGTCAAATGCTCTAAGGTAATGCTATTTGACCACAACGTGCCATCACGCGTAAGTCCAA +GGGAATATAGATCTTCCCCGGAGGCTGCACAGGAAGCGAGTACAACCACGTCACTGACGC +ATAGCCAATTCGACCTAAGCGTTGACGGCGAGATACTACCCGTCCCGTCAGATCTGGATG +CTGACGCCCCAGCCCTAGAACCAGCTCTAGACGACAGGGCGACACACACGTTGCCATCCA +CAACCGGAAACCTTGCGGCCGTGTCTGACTGGGTAATGAGCACCGTACCTGTCGCGCCGC +CCAGAAGAAGGCGAGGGAGAAACCTGACTGTGACATGCGACGAGAGAGAAGGGAACATAA +CACCCATGGCTAGCGTCCGATTCTTTAGGGCAGAGCTGCGTCCGGTCGTACAAGAAACAG +CGGAGACGCGTGACACAGCAATGTCTCTTCAGGCACCGCCGAGTACCGCCACGGAACTGA +ATCATCCGCCGATCTCCTTCGGAGCACCAAGCGAGACGTTCCCCATTACATTTGGGGACT +TCAACGAAGGAGAAATCGAAAGCTTGTCTTCTGAGCTGCTAACTTTCGGAGACTTCCTAC +CCGGAGAAGTGGATGACTTGACAGATAGCGACTGGTCCACGTGCTCAGACACGGACGACG +AGTTATGACTAGACAGGGCAGGTGGGTATATATTCTCGTCGGACACCGGTCCAGGTCATT +TACAACAGAAGTCAGTACGCCAGTCAGTGCTGCCGGTGAACACCCTGGAGGAAGTCCACG +AGGAGAAGTGTTACCCACCTAAGTTGGATGAAGTAAAGGAGCAACTATTACTTAAGAAAC +TCCAGGAGAGTGCATCCATGGCCAACAGAAGCAGGTACCAGTCGCGCAAAGTAGAAAACA +TGAAAGCAACAATCATCCAGAGACTAAAGAGAGGCTGTAGACTATACTTAATGTCAGAGA +CCTCAAAAGTCCCTACTTACCGGACCACATATCCGGCGCCTGTGTACTCGCCTCCGATCA +ACGTCCGACTGTCCAACCCCGAGTCCGCAGTGGCAGCATGCAATGAGTTCCTAGCTAGAA +ACTATCCAACTGTCTCATCATACCAAATTACCGACGAGTATGATGCATATTTAGACATGG +TGGACGGGTCGGAGAGTTGCCTGGATCGAGCGACATTCAATCCGTCAAAACTTAGGAGCT +ACCCGAAACAGCACGCTTACCATGCGCCCTCCATCAGAAGCGCTGTACCGTCCCCATTCC +AGAACACACTACAGAATGTACTGGCAGCAGCCACGAAAAGAAACTGCAACGTCACACAGA +TGAGGGAATTACCCACTTTGGACTCAGCAGTATTCAACGTGGAGTGTTTTAAAAAATTCG +CATGCAACCAAGAATACTGGGAAGAATTTGCTGCCAGCCCTATTAGGATAACAACTGAGA +ATTTAACAACCTATGTTACTAAACTAAAAGGGCCAAAAGCAGCAGCGCTATTTGCAAAAA +CCCACAATCTGCTGCCACTGCAGGAAGTACCAATGGATAGGTTCACAGTAGATATGAAAA +GGGACGTGAAGGTGACTCCTGGTACAAAGCACACAGAGGAAAGACCTAAGGTGCAGGTTA +TACAGGCGGCTGAACCCTTGGCGACAGCATACCTATGTGGGATTCACAGAGAGCTGGTTA +GGAGGCTGAACGCCGTCCTCCTACCCAATGTACATACACTATTTGATATGTCTGCCGAGG +ATTTCGATGCCATCATAGCCGCACACTTTAAGCCAGGAGACACTGTTTTAGAAACGGACA +TAGCCTCCTTTGACAAGAGCCAAGATGATTCACTTGCGCTTACTGCCTTGATGTTGTTAG +AAGATTTAGGGGTGGATCACTCCCTGTTGGACTTGATAGAGGCCGCTTTTGGAGAGATTT +CCAGCTGTCATCTACCGACAGGTACGCGCTTCAAGTTCGGCGCCATGATGAAATCTGGTA +TGTTCCTAACTCTGTTCGTCAACACATTGCTAAACATCACCATCGCTAGCCGCGTGCTGG +AAGATCGTCTGACAAAATCCGCGTGCGCGGCCTTCATCGGCGACGACAACATAATACATG +GAGTCGTCTCCGATGAATTGATGGCAGCCAGATGCGCCACTTGGATGAACATGGAAGTGA +AGATCATAGATGCAGTTGTATCCTTGAAAGCCCCTTACTTTTGTGGAGGGTTTATACTGC +ACGATACTGTGACGGGAACAGCCTGCAGAGTGGCAGACCCGCTAAAAAGGCTATTCAAAC +TGGGCAAACCGCTAGCGGCAGGTGACGAACAAGATGAAGATAGAAGACGAGCGCTGGCTG +ACGAAGTGATCAGATGGCAACGAACAGGGCTAATTGATGAGTTGGAGAAAGCGGTATACT +CTAGGTATGAAGTGCAGGGTATATCAGTTGTGGTAATGTCCATGGCCACCTTTGCAAGCT +CCAGATCTAACTTCGAGAAGCTCAGAGGACCCGTCATAACTTTGTACGGCGGTCCTAAAT +AGGTACGCACTACAGCTACCTATTTTGCAGAAGCCGACAGCAAGTACCTAAATACTAATT +AGCTACAATGGAGTACATCCCAACCCAAACTTTTTACAATAGGAGGTACCAGCCTCGACC +CTGGACTCCGCGCCCCACTATCCAAGTAATCAGGCCCAGACCGCGCCCTCAGAGGCAAGC +CGGGCAACTTGCCCAGCTGATCTCAGCAGTTAATAAACTGACAATGCGCGCGGTACCCCA +ACAGAAGCCACGCAGGAATCGGAAGAATAAGAAGCAAAAGCAAAAGCGACAGGCGCCACA +AAACAACACAAACCAAAAGAAGCAGCCGCCTAAAAAGAAACCAGCTCAAAAGAAAAAGAA +GCCGGGCCGCAGAGAGAGGATGTGCATGAAAATCGAAAATGATTGTATTTTTGAAGTCAA +GTATGAAGGTAAGGTAACAGGTTACGCGTGCTTGGTGGGGGACAAAGTAATGAAACCAGC +ACACGTAAAGGGGACCATCGATAACGCGGACCTGGCCAAACTGGCCTTTAAGCGGTCATC +TAAGTATGACCTTGAATGCGCGCAGATACCCGTGCACATGAAGTCCGACGCTTCGAAGTT +CACCCATGAGAAACCAGAGGGGTACTACAACTGGCACCACGGAGCAGTACAGTACTCAGG +AGGCCGGTTCACCATCCCCACAGGTGCTGGCAAACCAGGGGACAGCGGCAGACCGATCTT +CGACAACAAGGGACGCGTGGTGGCCATAGTCTTAGGAGGAGCTAATGAAGGAGCCCGTAC +AGCCCTCTCGGTGGTGACCTGGAATAAAGACATTGTCACTAAAATCACCCCCGAGGGGGC +CGAAGAGTGGAGTCTTGCCATCCCAGTCATGTGCCTGCTGGCAAATACCACGTTCCCCTG +CTCCCAGCCCCCTTGCACTCCCTGCTGCTATGAAAGGGAACCAGAGGAAACCCTACGCAT +GCTTGAGGACAACGTCATGAGACCTGGGTACTATCAGCTGCTACAAGCATCCTTAACATG +TTCTCCCCACCGTCAGCGACGCAGTACCAAGGACAACTTCAATGTCTATAAAGCCACAAG +ACCGTACTTAGCTCACTGTCCCGACTGTGGAGAAGGGCACTCGTGCCATAGTCCCGTAGC +ATTAGAACGCATCAGAAATGAAGCGACAGACGGGACGCTGAAAATCCAGGTCTCCTTGCA +AATCGGAATAAAGACGGATGATAGCCACGATTGGACCAAGCTGCGTTACATGGACAACCA +CACGCCAGCGGACGCAGAGAGGGCGGGGCTATTTGTAAGAACATCAGCACCGTGCACGAT +TACTGGAACAATGGGACACTTCATCCTGACCCGATGTCCGAAAGGGGAAACTCTGACGGT +GGGATTCACTGACAGTAGGAAGATCAGTCACTCATGTACGCACCCATTTCACCACGACCC +TCCTGTGATAGGCCGGGAGAAATTCCATTCCCGACCGCAGCACGGTAAAGAGCTGCCTTG +CAGCACGTACGTGCAGAGCACCGCCGCAACTACCGAGGAGATAGAGGTACACATGCCCCC +AGACACCCCTGATCGCACATTGATGTCACAACAGTCCGGCAACGTAAAGATCACAGTTAA +TGGCCAGACGGTGCGGTACAAGTGTAATTGCGGTGGCTCAAATGAAGGACTAATAACTAC +AGACAAAGTGATTAATAACTGCAAAGTTGATCAATGTCATGCCGCGGTCACCAATCACAA +AAAGTGGCAGTACAACTCCCCTCTGGTCCCGCGTAATGCTGAACTTGGGGACCGAAAAGG +AAAAATCCACATCCCGTTTCCGCTGGCAAATGTAACATGCAGGGTGCCTAAAGCAAGGAA +CCCCACCGTGACGTACGGGAAAAACCAAGTCATCATGCTACTGTATCCCGACCACCCAAC +ACTCCTGTCCTACCGGAACATGGGAGAAGAACCAAACTACCAAGAAGAGTGGGTGACGCA +TAAGAAGGAAGTCGTGCTAACCGTGCCGACTGAAGGGCTCGAGGTCACGTGGGGTAACAA +CGAGCCGTATAAGTATTGGCCGCAGTTATCTACAAACGGTACAGCCCATGGCCACCCGCA +TGAGATAATTCTGTATTATTATGAGCTGTACCCTACTATGACTGTAGTAGTTGTGTCAGT +GGCCTCGTTCGTACTCCTGTCGATGGTGGGTGTGGCAGTGGGGATGTGCATGTGTGCACG +ACGCAGATGCATCACACCGTACGAACTGACACCAGGAGCTACCGTCCCTTTCCTGCTTAG +CCTAATATGCTGCATCAGAACAGCTAAAGCGGCCACATACCAAGAGGCTGCGGTATACCT +GTGGAATGAGCAGCAACCTTTGTTTTGGCTACAAGCCCTTATTCCGCTGGCAGCCCTGAT +TGTCCTATGCAACTGTCTGAGACTCTTACCATGCTGCTGTAAAACGTTGGCTTTTTTAGC +CGTAATGAGCGTCGGTGCCCACACTGTGAGCGCGTACGAACACGTAACAGTGATCCCGAA +CACGGTGGGAGTACCGTATAAGACTCTAGTCAATAGACCGGGCTACAGTCCCATGGTATT +GGAGATGGAACTACTGTCAGTCACTTTGGAGCCAACGCTATCGCTTGATTACATCACGTG +CGAGTACAAAACCGTTATCCCGTCTCCGTACGTGAAATGCTGCGGTACAGCAGAGTGCAA +GGACAAAAACCTACCTGACTACAGCTGTAAGGTCTTCACCGGCGTCTACCCATTTATGTG +GGGCGGAGCCTACTGCTTCTGCGACGCTGAAAACACGCAATTGAGCGAAGCACACGTGGA +GAAGTCCGAATCATGCAAAACAGAATTTGCATCAGCATACAGGGCTCATACCGCATCCGC +ATCAGCTAAGCTCCGCGTCCTTTACCAAGGAAATAACATCACTGTAACTGCCTATGCTAA +CGGCGACCATGCCGTCACAGTTAAGGACGCCAAATTCATTGTGGGGCCAATGTCTTCAGC +CTGGACACCTTTCGACAACAAAATTGTGGTGTACAAAGGTGACGTCTATAACATGGACTA +CCCGCCCTTTGGCGCAGGAAGACCAGGACAATTTGGCGATATCCAAAGTCGCACACCTGA +GAGTAAAGACGTCTATGCTAATACACAACTGGTACTGCAGAGACCGGCTGCGGGTACGGT +ACATGTGCCATACTCTCAGGCACCATCTGGCTTTAAGTATTGGCTAAAAGAACGAGGGGC +GTCGCTGCAGCACACAGCACCATTTGGCTGCCAAATAGCAACAAACCCGGTAAGAGCGGT +GAATTGCGCCGTAGGGAACATGCCCATCTCCATCGACATACCGGATGCGGCCTTCATTAG +GGTCGTCGACGCGCCCTCTTTAACGGACATGTCGTGCGAGGTACCAGCCTGCACCCATTC +CTCAGATTTCGGGGGCGTCGCCATTATTAAATATGCAGCCAGCAAGAAAGGCAAGTGTGC +GGTGCATTCGATGACCAACGCCGTCACAATTCGGGAAGCTGAGATAGAAGTTGAAGGGAA +TTCTCAGCTGCAAATCTCTTTCTCGACGGCCTTGGCCAGCGCCGAATTCCGCGTACAAGT +CTGTTCTACACAAGTACACTGTGTAGCCGAGTGCCACCCTCCGAAGGACCACATAGTCAA +TTACCCGGCGTCACATACCACCCTCGGGGTCCAGGACATTTCCGCTACGGCGCTGTCATG +GGTGCAGAAGATCACGGGAGGCGTGGGACTGGTTGTCGCTGTTGCAGCACTGATTCTAAT +CGTGGTGCTATGCGTGTCGTTCAGCAGGCACTAACTTGACAACTAAGTATGAAGGTATAT +GTGTCCCCTAAGAGACACACTGTAAATAGCTAATAATCTATAGATCAAAGGGCTATACAA +CCCCTGAATAGTAACAAAATACAAAATCACTAAAAATTATAAAAACAGAAAAATACATAA +ATAGGTATACGTGTCCCCTAAGAGACACATTGTATGTAGGTGATAAGTATAGATCAAAGG +GCCGAACAACCCCTGAATAGTAACAAAACATGAAAATCAATAAAAATCATAAAATAGAAA +AACCATAAACAGAAGTAGTTCAAAGGGCTATACAACCCCTGAATAGTAACAAAACACAAA +ACTAATAAAAATTAAATGAATACCATAATTGGCAAACGGAAGAGATGTAGGTACTTAAGC +TTCCTAAAAGCAGCCGAACTCACTTTGAGATGTAGGCATAGCATACCGAACTCTTCCACG +ATTCTCCGAACCCACAGGGACGTAGGAGATGTTATTTTGTTTTTAATATTTC