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Dockerfile
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FROM ubuntu:14.04
RUN apt-get update && apt-get install -y \
autoconf \
automake \
make \
g++ \
gcc \
build-essential \
zlib1g-dev \
libgsl0-dev \
perl \
curl \
git \
wget \
unzip \
tabix \
libncurses5-dev
RUN apt-get install -y cpanminus
RUN apt-get install -y libmysqlclient-dev
RUN cpanm CPAN::Meta \
Archive::Zip \
DBI \
DBD::mysql \
JSON \
DBD::SQLite \
Set::IntervalTree \
LWP \
LWP::Simple \
Archive::Extract \
Archive::Tar \
Archive::Zip \
CGI \
Time::HiRes \
Encode \
File::Copy::Recursive \
Perl::OSType \
Module::Metadata version \
Bio::Root::Version \
TAP::Harness \
Module::Build
WORKDIR /opt
RUN wget https://github.com/samtools/samtools/releases/download/1.3/samtools-1.3.tar.bz2
RUN tar jxf samtools-1.3.tar.bz2
WORKDIR /opt/samtools-1.3
RUN make
RUN make install
WORKDIR /opt
RUN rm samtools-1.3.tar.bz2
RUN wget https://github.com/Ensembl/ensembl-tools/archive/release/85.zip
RUN mkdir variant_effect_predictor_85
RUN mkdir variant_effect_predictor_85/cache
RUN unzip 85.zip -d variant_effect_predictor_85
RUN rm 85.zip
WORKDIR /opt/variant_effect_predictor_85/ensembl-tools-release-85/scripts/variant_effect_predictor/
RUN perl INSTALL.pl --AUTO ap --PLUGINS LoF --CACHEDIR /opt/variant_effect_predictor_85/cache
WORKDIR /opt/variant_effect_predictor_85/cache/Plugins
RUN wget https://raw.githubusercontent.com/konradjk/loftee/master/splice_module.pl
WORKDIR /opt
ADD . /opt/vcf2maf
COPY Dockerfile /opt/
MAINTAINER Michele Mattioni, Seven Bridges, <[email protected]>