Code-base for Srikant S et al, Selecting for Altered Substrate Specificity Reveals the Evolutionary Flexibility of ATP-Binding Cassette Transporters, Current Biology (2020); (https://doi.org/10.1016/j.cub.2020.02.077)
Dataset for the paper found in "MendeleySourceData" folder.
Reproduced from (https://doi.org/10.17632/rp3hnvh494.1)
This is the accompanying data for the Current Biology article titled "Selecting for altered substrate specificity reveals the evolutionary flexibility of ATP-binding cassette transporters" authored by Sriram Srikant, Rachelle Gaudet and Andrew W Murray (https://doi.org/10.1016/j.cub.2020.02.077). The sequence alignment and homology models presented here support the inferences made in our manuscript. The autocrine data presented here is the raw data supporting our figures.
Data index:
- MendeleySourceDataFile1.fasta | Alignment of fungal pheromone ABC exporters focused on Y. lipolytica Ste6.
- MendeleySourceDataFile2.pdb | Homology model of YlSte6 made based on inward-open LTC4-bound BtMRP1 (PDB:5UJA).
- MendeleySourceDataFile3.pdb | Homology model of YlSte6 made based on outward-open ATP-bound BtMRP1 (PDB:6BHU).
- MendeleySourceDataFile4.xlsx | Autocrine data of ScSte6, YlSte6, selected clones and mutational combinations of YlSte6 used to test additive model (Figure 6).
- MendeleySourceDataFile5.csv | Autocrine data of all selected clones tested by flow cytometry (Figure S2 and S3).