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Navigate to https://blast.ncbi.nlm.nih.gov/Blast.cgi
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Data:
ATGGCACAATTATTTTTTAAATATGGCGCAATGAACAGTGGGAAAACAATTGAAATTTTGAAAGTTGCCC
ACAATTATGAAGAGCAAGATAAACCTGTTGTGATTATGACAAGTGGTTTAGATACGCGTTCTGGAGTAGG
GCGAGTCTCAAGTCGAATCGGATTGGAAAGAGAAGCATTGCCGATTTTTGCTGAAACTAACGATTTTGAA
ACGATTGTAAATTTAGAATTAAAACCCTACTGTGTGCTAATCGACGAAGCACAATTTTTGCAAAAACAGC
ATGTTTTGGACTTTACTAGAATTGTAGATGAATTGAATATTCCAGTAATGGCCTTTGGCTTAAAAAATGA
TTTTCGCAATGAGTTATTTGAAGGATCTAAATATTTACTTTTATATGCTGACAAAATTGAAGAAATGAAA
ACAATCTGTTGGTTTTGTCATAAAAAAGCGATTATGAACCTTCATTATATTGATGGAAAACCAGTCTATG
AAGGAAACCAAGTCCAAATTGGGGGCAACGAAGCGTATTATCCTGTGTGCCGACACCATTATTTCCATCC
AGAAATATAA
- For the sequence above, perform one or more "flavors" of BLAST (blastn, blastp, blastx, etc) as you think appropriate to:
- Determine what protein/gene the sequence has homology to.
- Identify the organism the sequence is likely to have come from.
- Determine the taxonomic order of the organism.
Make a record of the evidence that has led you to each of these conclusions. Make sure to record your search strategy/strategies, such that your process can be reproduced.
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Navigate to https://www.uniprot.org
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Use the search function to find the entry for the protein (in the organism) you identified in #3.
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Using the advanced search function: Build a query that will find this protein in all members of the taxonomic order that the organism belongs to.
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Now filter your search to only display Reviewed (Swiss-Prot) entries. View some of these entries. What type of information can you learn about the protein through these entries that was not available on the original entry?
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Start a new advanced search. This time search for all proteins in the organism of interest.
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Download a fasta file of the search (save this for use on Day 2).