agg func is optional to support base pair level data for each input region

src/genomicarrays/GenomicArrayDataset.py

@@ -64,12 +64,8 @@ def __init__(
         self._dataset_path = dataset_path
         # TODO: Maybe switch to on-demand loading of these objects
         self._matrix_tdb_tdb = tiledb.open(f"{dataset_path}/{matrix_tdb_uri}", "r")
-        self._feature_annotation_tdb = tiledb.open(
-            f"{dataset_path}/{feature_annotation_uri}", "r"
-        )
-        self._sample_metadata_tdb = tiledb.open(
-            f"{dataset_path}/{sample_metadata_uri}", "r"
-        )
+        self._feature_annotation_tdb = tiledb.open(f"{dataset_path}/{feature_annotation_uri}", "r")
+        self._sample_metadata_tdb = tiledb.open(f"{dataset_path}/{sample_metadata_uri}", "r")
 
     def __del__(self):
         self._matrix_tdb_tdb.close()
@@ -110,9 +106,7 @@ def get_feature_annotation_index(self) -> List[str]:
         res = qtd.get_a_column(self._feature_annotation_tdb, "genarr_feature_index")
         return res["genarr_feature_index"].tolist()
 
-    def get_feature_subset(
-        self, subset: Union[slice, List[str], tiledb.QueryCondition], columns=None
-    ) -> pd.DataFrame:
+    def get_feature_subset(self, subset: Union[slice, List[str], tiledb.QueryCondition], columns=None) -> pd.DataFrame:
         """Slice the ``feature_annotation`` store.
 
         Args:
@@ -147,16 +141,12 @@ def get_feature_subset(
                     _not_avail.append(col)
 
             if len(_not_avail) > 0:
-                raise ValueError(
-                    f"Columns '{', '.join(_not_avail)}' are not available."
-                )
+                raise ValueError(f"Columns '{', '.join(_not_avail)}' are not available.")
 
         if qtd._is_list_strings(subset):
             subset = self._get_indices_for_gene_list(subset)
 
-        return qtd.subset_frame(
-            self._feature_annotation_tdb, subset=subset, columns=columns
-        )
+        return qtd.subset_frame(self._feature_annotation_tdb, subset=subset, columns=columns)
 
     ####
     ## Subset methods for the `sample_metadata` TileDB file.
@@ -183,9 +173,7 @@ def get_sample_metadata_column(self, column_name: str) -> pd.DataFrame:
         res = qtd.get_a_column(self._sample_metadata_tdb, column_name=column_name)
         return res[column_name]
 
-    def get_sample_subset(
-        self, subset: Union[slice, tiledb.QueryCondition], columns=None
-    ) -> pd.DataFrame:
+    def get_sample_subset(self, subset: Union[slice, tiledb.QueryCondition], columns=None) -> pd.DataFrame:
         """Slice the ``sample_metadata`` store.
 
         Args:
@@ -216,13 +204,9 @@ def get_sample_subset(
                     _not_avail.append(col)
 
             if len(_not_avail) > 0:
-                raise ValueError(
-                    f"Columns '{', '.join(_not_avail)}' are not available."
-                )
+                raise ValueError(f"Columns '{', '.join(_not_avail)}' are not available.")
 
-        return qtd.subset_frame(
-            self._sample_metadata_tdb, subset=subset, columns=columns
-        )
+        return qtd.subset_frame(self._sample_metadata_tdb, subset=subset, columns=columns)
 
     ####
     ## Subset methods for the `matrix` TileDB file.
@@ -266,16 +250,14 @@ def get_matrix_subset(self, subset: Union[int, Sequence, tuple]) -> pd.DataFrame
                     shape=(len(subset[0]), len(subset[1])),
                 )
             else:
-                raise ValueError(
-                    f"`{type(self).__name__}` only supports 2-dimensional slicing."
-                )
+                raise ValueError(f"`{type(self).__name__}` only supports 2-dimensional slicing.")
 
     ####
     ## Subset methods by cell and gene dimensions.
     ####
     def get_slice(
         self,
-        feature_subset: Union[slice, tiledb.QueryCondition],
+        feature_subset: Union[slice, int],
         sample_subset: Union[slice, List[str], tiledb.QueryCondition],
     ) -> GenomicArrayDatasetSlice:
         """Subset a ``GenomicArrayDataset``.
@@ -297,14 +279,24 @@ def get_slice(
         _ssubset = self.get_sample_subset(sample_subset)
         _sample_indices = _ssubset.index.tolist()
 
+        if not isinstance(feature_subset, (int, slice)):
+            raise TypeError("feature indices must be continous; either a 'slice' or 'int' index.")
         _fsubset = self.get_feature_subset(feature_subset)
-        _feature_indices = _fsubset.index.tolist()
+        start_findex = _fsubset["genarr_feature_start_index"].astype(int).min()
+        end_findex = _fsubset["genarr_feature_end_index"].astype(int).max()
+
+        # expand intervals
+        final_rows = []
+        for row in _fsubset.itertuples():
+            for i, _ in enumerate(range(int(row.genarr_feature_start_index), int(row.genarr_feature_end_index))):
+                final_rows.append(row._replace(starts=i + row.starts, ends=i + row.starts + 1))
+        _feature_df = pd.DataFrame(final_rows)
 
-        _msubset = self.get_matrix_subset((_feature_indices, _sample_indices))
+        _msubset = self.get_matrix_subset((list(range(start_findex, end_findex)), _sample_indices))
 
         return GenomicArrayDatasetSlice(
             _ssubset,
-            _fsubset,
+            _feature_df,
             _msubset,
         )
 
@@ -351,13 +343,9 @@ def __getitem__(
             elif len(args) == 2:
                 return self.get_slice(args[0], args[1])
             else:
-                raise ValueError(
-                    f"`{type(self).__name__}` only supports 2-dimensional slicing."
-                )
+                raise ValueError(f"`{type(self).__name__}` only supports 2-dimensional slicing.")
 
-        raise TypeError(
-            "args must be a sequence or a scalar integer or string or a tuple of atmost 2 values."
-        )
+        raise TypeError("args must be a sequence or a scalar integer or string or a tuple of atmost 2 values.")
 
     ####
     ## Misc methods.

src/genomicarrays/build_genomicarray.py

@@ -174,7 +174,29 @@ def build_genomicarray(
             missing = required_cols - set(input_intervals.columns)
             raise ValueError(f"Missing required columns: {missing}")
     else:
-        raise TypeError("'input_intervals' is not an expected type (either 'str' or 'Dataframe').")
+        raise TypeError(
+            "'input_intervals' is not an expected type (either 'str' or 'Dataframe')."
+        )
+
+    # append start index for each interval
+    input_intervals["widths"] = input_intervals["ends"] - input_intervals["starts"]
+    total_length = None
+    if feature_annotation_options.aggregate_function is None:
+        total_length = int(input_intervals["widths"].sum())
+        counter = input_intervals["widths"].shift(1)
+        counter[0] = 0
+        input_intervals["genarr_feature_start_index"] = counter.cumsum().astype(int)
+    else:
+        counter = [1] * len(input_intervals)
+        total_length = len(input_intervals)
+        counter[0] = 0
+        input_intervals["genarr_feature_start_index"] = (
+            pd.Series(counter).cumsum().astype(int)
+        )
+
+    ends = input_intervals["genarr_feature_start_index"].shift(-1)
+    ends.iloc[-1] = total_length
+    input_intervals["genarr_feature_end_index"] = ends.astype(int)
 
     if not feature_annotation_options.skip:
 
@@ -187,13 +209,19 @@ def build_genomicarray(
 
             input_intervals["sequences"] = sequences
 
-        _col_types = utf.infer_column_types(input_intervals, feature_annotation_options.column_types)
+        _col_types = utf.infer_column_types(
+            input_intervals, feature_annotation_options.column_types
+        )
 
         if "genarr_feature_index" not in input_intervals.columns:
             input_intervals["genarr_feature_index"] = range(0, len(input_intervals))
 
-        _feature_output_uri = f"{output_path}/{feature_annotation_options.tiledb_store_name}"
-        utf.create_tiledb_frame_from_dataframe(_feature_output_uri, input_intervals, column_types=_col_types)
+        _feature_output_uri = (
+            f"{output_path}/{feature_annotation_options.tiledb_store_name}"
+        )
+        utf.create_tiledb_frame_from_dataframe(
+            _feature_output_uri, input_intervals, column_types=_col_types
+        )
 
         if optimize_tiledb:
             uta.optimize_tiledb_array(_feature_output_uri)
@@ -223,10 +251,16 @@ def build_genomicarray(
         raise TypeError("'sample_metadata' is not an expected type.")
 
     if not sample_metadata_options.skip:
-        _col_types = utf.infer_column_types(sample_metadata, sample_metadata_options.column_types)
+        _col_types = utf.infer_column_types(
+            sample_metadata, sample_metadata_options.column_types
+        )
 
-        _sample_output_uri = f"{output_path}/{sample_metadata_options.tiledb_store_name}"
-        utf.create_tiledb_frame_from_dataframe(_sample_output_uri, sample_metadata, column_types=_col_types)
+        _sample_output_uri = (
+            f"{output_path}/{sample_metadata_options.tiledb_store_name}"
+        )
+        utf.create_tiledb_frame_from_dataframe(
+            _sample_output_uri, sample_metadata, column_types=_col_types
+        )
 
         if optimize_tiledb:
             uta.optimize_tiledb_array(_sample_output_uri)
@@ -242,7 +276,7 @@ def build_genomicarray(
             x_dim_dtype=feature_annotation_options.dtype,
             y_dim_dtype=sample_metadata_options.dtype,
             matrix_dim_dtype=matrix_options.dtype,
-            x_dim_length=len(input_intervals),
+            x_dim_length=total_length,
             y_dim_length=len(files),
             is_sparse=False,
         )
@@ -254,6 +288,7 @@ def build_genomicarray(
                 bwpath,
                 idx,
                 feature_annotation_options.aggregate_function,
+                total_length,
             )
             for idx, bwpath in enumerate(files)
         ]
@@ -276,16 +311,25 @@ def build_genomicarray(
     )
 
 
-def _write_intervals_to_tiledb(outpath, intervals, bwpath, bwidx, agg_func):
+def _write_intervals_to_tiledb(
+    outpath, intervals, bwpath, bwidx, agg_func, total_length
+):
     """Wrapper to extract the data for the given intervals from the bigwig file and write the output to the tiledb
     file."""
-    data = ubw.extract_bw_intervals_as_vec(bwpath, intervals, agg_func)
+    data = ubw.wrapper_extract_bw_values(
+        bw_path=bwpath,
+        intervals=intervals,
+        agg_func=agg_func,
+        total_length=total_length,
+    )
 
     if data is not None and len(data) > 0:
         uta.write_frame_intervals_to_tiledb(outpath, data=data, y_idx=bwidx)
 
 
 def _wrapper_extract_bwinfo(args):
     """Wrapper for multiprocessing multiple files and intervals."""
-    counts_uri, input_intervals, bwpath, idx, agg_func = args
-    return _write_intervals_to_tiledb(counts_uri, input_intervals, bwpath, idx, agg_func)
+    counts_uri, input_intervals, bwpath, idx, agg_func, total_length = args
+    return _write_intervals_to_tiledb(
+        counts_uri, input_intervals, bwpath, idx, agg_func, total_length
+    )

src/genomicarrays/build_options.py

@@ -1,5 +1,5 @@
 from dataclasses import dataclass
-from typing import Dict, Literal
+from typing import Dict, Literal, Optional, Callable
 
 import numpy as np
 
@@ -122,14 +122,14 @@ class FeatureAnnotationOptions:
 
         aggregate_function:
             A callable to summarize the values in a given
-            interval. Defaults to NumPy's `nanmean`.
+            interval. Defaults to None.
     """
 
     skip: bool = False
     dtype: np.dtype = np.uint32
     tiledb_store_name: str = "feature_annotation"
     column_types: Dict[str, np.dtype] = None
-    aggregate_function: callable = np.nanmean
+    aggregate_function: Optional[Callable] = None
 
     def __post_init__(self):
         if self.column_types is None:

src/genomicarrays/utils_bw.py

@@ -1,4 +1,4 @@
-from typing import Tuple
+from typing import Optional, Tuple
 
 import numpy as np
 import pandas as pd
@@ -37,10 +37,28 @@ def extract_bw_values(
 #     )
 
 
-def extract_bw_intervals_as_vec(
+def wrapper_extract_bw_values(
     bw_path: str,
     intervals: pd.DataFrame,
-    agg_func: callable = np.nanmean,
+    agg_func: Optional[callable],
+    val_dtype: np.dtype = np.float32,
+    total_length: int = None,
+) -> np.ndarray:
+    if total_length is None:
+        total_length = len(intervals)
+
+    if agg_func is not None:
+        return extract_bw_values_as_vec(bw_path=bw_path, intervals=intervals, agg_func=agg_func, val_dtype=val_dtype)
+    else:
+        return extract_bw_intervals_as_vec(
+            bw_path=bw_path, intervals=intervals, val_dtype=val_dtype, total_length=total_length
+        )
+
+
+def extract_bw_values_as_vec(
+    bw_path: str,
+    intervals: pd.DataFrame,
+    agg_func: Optional[callable] = None,
     val_dtype: np.dtype = np.float32,
 ) -> np.ndarray:
     """Extract data from BigWig for a given region and apply the aggregate function.
@@ -54,7 +72,7 @@ def extract_bw_intervals_as_vec(
 
         agg_func:
             Aggregate function to apply.
-            Defaults to np.nanmean.
+            Defaults to None.
 
         val_dtype:
             Dtype of the resulting array.
@@ -81,3 +99,60 @@ def extract_bw_intervals_as_vec(
             results.append(np.nan)
 
     return np.array(results, dtype=val_dtype)
+
+
+def _get_empty_array(size, val_dtype):
+    out_array = np.empty(size, dtype=val_dtype)
+    out_array.fill(np.nan)
+
+    return out_array
+
+
+def extract_bw_intervals_as_vec(
+    bw_path: str,
+    intervals: pd.DataFrame,
+    total_length: int,
+    val_dtype: np.dtype = np.float32,
+) -> np.ndarray:
+    """Extract data from BigWig for a given region.
+
+    Args:
+        bw_path:
+            Path to the BigWig file.
+
+        intervals:
+            List of intervals to extract.
+
+        total_length:
+            Size of all the regions.
+
+        val_dtype:
+            Dtype of the resulting array.
+
+    Returns:
+        A vector with length as the number of intervals,
+        a value if the file contains the data for the corresponding
+        region or ``np.nan`` if the region is not measured.
+    """
+    bwfile = bw.open(bw_path)
+
+    out_array = _get_empty_array(total_length, val_dtype=val_dtype)
+
+    for row in intervals.itertuples():
+        if row.seqnames in bwfile.chroms():
+            try:
+                data = bwfile.intervals(row.seqnames, row.starts, row.ends)
+                tmp_out = _get_empty_array(row.ends - row.starts, val_dtype=val_dtype)
+                if data is not None and len(data) != 0:
+                    for d in data:
+                        _strt = max(0, d[0] - row.starts)
+                        _end = min(d[1] - row.starts, row.ends)
+                        tmp_out[_strt:_end] = d[2]
+
+                out_array[row.genarr_feature_index_start : row.genarr_feature_index_start + row.ends - row.starts] = (
+                    tmp_out
+                )
+            except Exception as _:
+                pass
+
+    return out_array