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A tool to predict library loading concentration from cluster density and bioanalyzer data.

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JBEI/library_loading_concentration_prediction

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DIVA DNA Seq Utilities

This package is a repository of tools used by the DIVA DNA Seq team at the Joint BioEnergy Institute (JBEI) to predict the library loading concentration for their MiSeq runs. It employs a machine learning model to determine the relationship between library fragment sizes, loading conentration and cluster density.

Installation

The tool can be installed using pip with

pip install diva_dna_seq

Use

This tool can then be used either in a Jupyter notebook or on the command line. A bioanalyzer ladder file and result file are needed. The tool generates a plot that relates library loading concentration for your library to the MiSeq flow cell cluster density.