Final Work: Applied Bioinformatics in Medical Genomics

Group:

• Julia Beltramini
• Kaira Cristina Peralis Tomaz
• Leonardo V. Azevedo
• Mélcar Collodetti
• Sara Reis
• Victor Aldair

Clinical Description of the Patient:

Patient with acute respiratory syndrome with recent use of the antibiotic Azithromycin and the drug Ivermectin.

Overall Objective:

This work aims to analyze the microbial community present in the sample through omics techniques and the identification of possible pathogens.

Specific Objectives:

Execute the three pipelines provided by the instructors to solve the patient's case.

Material Provided:

RNA Virome (Metatranscriptomics or Total RNA)

• VIROMA-R1: (https://aulas-pos-hiae-public-data.s3.sa-east-1.amazonaws.com/TCC-metagenomica/patient_joao_VIROMA_S21_R1_001.fastq.gz)
• VIROMA-R2: (https://aulas-pos-hiae-public-data.s3.sa-east-1.amazonaws.com/TCC-metagenomica/patient_joao_VIROMA_S21_R2_001.fastq.gz)

Questions:

1. What pathogenic virus is present in the sample? What can you say about its genomic and lineage characteristics?
2. Is there any virus that could be considered an incidental finding? Which one and why?