Bulk-RNAseq Workflow Landing Page

Sequencing, Files, and Data Pre-processing

• FastQ File Quality Control with FastQC
• FastQ File Trimming with FastP
• Check RNA-seq Strandness with RSeQC
• Align RNA-seq Reads to a Reference Genome with HISAT2
• Get Counts and Create a Counts Matrix with featureCounts
• Gene Set Overlap Significance Testing in R