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update version && add simulation of long read data
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moold committed Jun 16, 2020
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[![Downloads](https://img.shields.io/github/downloads/Nextomics/NextPolish/total?logo=github)](https://github.com/Nextomics/NextPolish/releases/download/v1.2.1/NextPolish.tgz)
[![Downloads](https://img.shields.io/github/downloads/Nextomics/NextPolish/total?logo=github)](https://github.com/Nextomics/NextPolish/releases/download/v1.2.2/NextPolish.tgz)
[![Release](https://img.shields.io/github/release/Nextomics/NextPolish.svg)](https://github.com/Nextomics/NextPolish/releases)
[![Issues](https://img.shields.io/github/issues/Nextomics/NextPolish.svg)](https://github.com/Nextomics/NextPolish/issues)

# NextPolish
NextPolish is used to fix base errors (SNV/Indel) in the genome generated by noisy long reads, it can be used with short read data only or long read data only or a combination of both. It contains two core modules, and use a stepwise fashion to correct the error bases in reference genome. To correct the raw third-generation sequencing (TGS) long reads with approximately 15-10% sequencing errors, please use [NextDenovo](https://github.com/Nextomics/NextDenovo).

* **DOWNLOAD**
click [here](https://github.com/Nextomics/NextPolish/releases/download/v1.2.1/NextPolish.tgz) or use the following command:
`wget https://github.com/Nextomics/NextPolish/releases/download/v1.2.1/NextPolish.tgz`
click [here](https://github.com/Nextomics/NextPolish/releases/download/v1.2.2/NextPolish.tgz) or use the following command:
`wget https://github.com/Nextomics/NextPolish/releases/download/v1.2.2/NextPolish.tgz`

* **REQUIREMENT**
* [Python](https://www.python.org/download/releases/) (Support python 2 and 3)
* [Psutil](https://psutil.readthedocs.io/en/latest/)
* [Drmaa](https://github.com/pygridtools/drmaa-python) (Only required by running under non-local system)
* **REQUIREMENT**
* [Python](https://www.python.org/download/releases/) (Support python 2 and 3):
* [Psutil](https://psutil.readthedocs.io/en/latest/)
* [Drmaa](https://github.com/pygridtools/drmaa-python) (Only required by running under non-local system)

* **INSTALL**
`tar -vxzf NextPolish.tgz && cd NextPolish && make`
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* **USAGE**
Please see [doc/OPTION.md](doc/OPTION.md) for options introduction.

* **PERFORMANCE COMPARISION**
+ [Simulation data](./doc/TEST1.md)
* **PERFORMANCE COMPARISION**
+ [Simulation of long read data](./doc/TEST3.md)
+ [Simulation of short read data](./doc/TEST1.md)
+ [Actual biological data](./doc/TEST2.md)

* **HELP**
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9. RuntimeError: Could not find drmaa library. Please specify its full path using the environment variable DRMAA_LIBRARY_PATH.
Please setup the environment variable: DRMAA_LIBRARY_PATH, see [here](https://github.com/pygridtools/drmaa-python) for more details.
10. OSError: /path/lib64/libc.so.6: version `GLIBC_2.14' not found (required by /path/NextPolish/lib/calgs.so).
Please download [this version](https://github.com/Nextomics/NextPolish/releases/download/v1.2.1/NextPolish-CentOS6.9.tgz) and try again.
Please download [this version](https://github.com/Nextomics/NextPolish/releases/download/v1.2.2/NextPolish-CentOS6.9.tgz) and try again.

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