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Material for the course STA 426 // Fall Semester 2022 // UZH

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Statistical Analysis of High-Throughput Genomic and Transcriptomic Data

Fall/Herbst-semester 2022

Lectures

Mondays 9.00-9.45 (Y27-H-46), 10.00-10.45 (Y27-H-46)

Exercises

Monday 11.00-11.45 (Y01-F-50)

Lecturers

Dr. Hubert Rehrauer, Group Leader of Genome Informatics at FGCZ

Prof. Dr. Mark Robinson, Associate Professor of Statistical Genomics, DMLS, UZH

Schedule

Date Lecturer Topic Exercise JC1 JC2
19.09.2022 Mark + Hubert admin; mol. bio. basics quarto; git(hub)
26.09.2022 Mark interactive technology/statistics session group exercise: technology pull request
03.10.2022 Hubert NGS intro; exploratory data analysis EDA in R
10.10.2022 Mark limma + friends linear model simulation + design matrices
17.10.2022 Hubert mapping Rsubread
24.10.2022 Hubert RNA-seq quantification RSEM Capturing Heterogeneity in Gene Expression Studies by Surrogate Variable Analysis JK DW X
31.10.2022 Mark edgeR+friends 1 basic edgeR/voom X X
07.11.2022 Mark edgeR+friends 2 advanced edgeR/voom X X
14.11.2022 YYY hands-on session #1: RNA-seq FASTQC/Salmon/etc. X X
21.11.2022 Hubert single-cell 1: preprocessing, dim. reduction, clustering clustering X Comparison of Transformations for Single-Cell RNA-Seq Data (GA, RE)
28.11.2022 YYY hands-on session #2: cytometry cytof null comparison Integrated analysis of multimodal single-cell data (ME, FR) X
05.12.2022 Mark single-cell 2: clustering, marker gene DE marker gene DE Differential abundance testing on single-cell data using k-nearest neighbor graphs (DK) X
12.12.2022 YYY hands-on session #3: single-cell RNA-seq (cell type definition, differential state) full scRNA-seq pipeline X Removing unwanted variation from large-scale RNA sequencing data with PRPS (VH, MP)
19.12.2022 Mark loose ends: HMM, EM, robustness segmentation, peak finding X X

Course material

Assuming you have git installed locally, you can check out the entire set of course materials with the following command (from command line):

git clone https://github.com/sta426hs2022/material.git

and get updates by running git pull at any later time in the same directory.

Alternatively, to retrieve a ZIP file of the repository, you can click on the (green) 'Code' button (top right of main panel) and then click 'Download ZIP'.

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Material for the course STA 426 // Fall Semester 2022 // UZH

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