sashimi group

[Qingru44004]

Hi,

Does the sashimi plot support drawing more than 2 groups by customization? If not, is there any other way that I can do it? Thanks.

Best,
Qingru

[EricKutschera]

Yes, you can add more than 2 groups with --group-info. The example in the README could be modified to have three groups with grouping.gf changed to:

group1name: 1-2
group2name: 3-4
group3name: 5-6

https://github.com/Xinglab/rmats2sashimiplot/tree/v3.0.0?tab=readme-ov-file#using-a-group-file

[Qingru44004]

Thanks. If I have three groups of data. I did rmats on each pair. Now, I want to plot sashimi on the common events of these three groups. How should I do it?

[EricKutschera]

You can run all of the data in the same post step. Then you'll have one set of output files with all of the events and the read counts for each sample: https://github.com/Xinglab/rmats-turbo/tree/v4.3.0?tab=readme-ov-file#running-the-statistical-model-separately