Materials on the analysis of microarray expression data; focus on re-analysis of public data
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Intro to microarray technology (Illumina / affy) & Data analysis
- probes, target terminology
- Bioconductor introduction
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Prac; 1st look at microarray data and R refresher
- Existing Bioc practical
-Specific technologies + Affy workflow + Illumina workflow + Diagnostic plots + Quality control / normalisation
- Linear models and statistics for differential expression
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Practical on differential expression
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Advanced aspects of analysis
- Annotation
- Batch effects
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Clustering, PCA, (survival?)
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Existing prac
Use this?
source("https://bioconductor.org/biocLite.R") biocLite("Affyhgu133Plus2Expr")