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Merge pull request #3 from csc-training/IonTorrentJYU23
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Minor change to Ion Torrent day 1
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helijuottonen committed Aug 14, 2023
2 parents cbe02f4 + b0913c0 commit 25f19c7
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7 changes: 3 additions & 4 deletions docs/IonTorrent/Exercises_day1.html
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<title>Exercises_day1.knit</title>
<title>Exercises_IonTorrent_day1.knit</title>

<script>// Pandoc 2.9 adds attributes on both header and div. We remove the former (to
// be compatible with the behavior of Pandoc < 2.8).
Expand Down Expand Up @@ -891,13 +891,12 @@ <h1><strong>Day 1: Data pre-processing</strong></h1>
<p>Note that after this point in real life you can delete the individual
FASTQ files, because you can always open the tar package using the tool
<code>Utilities / Extract .tar.gz file</code>.</p>
<p><strong>Step 3. Check the quality of reads with FastQC</strong></p>
<p><strong>Step 3. Check the quality of reads with MultiQC</strong></p>
<p>Select the file <code>zippedFastq.tar</code> and the tool
<code>Quality control / Read quality with MultiQC for many FASTQ files</code>,
and click <em>Run</em>. Select the result file and click <em>Open in new
tab</em>.</p>
<pre><code>How long are the reads (in the General Statistics section, click
Configure columns, select Length and unselect M sequences)?
<pre><code>How long are the reads?

Based on the plot Sequence Counts, do all the samples have the
same number of reads? Are most of the reads unique?
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5 changes: 2 additions & 3 deletions eLena_md/IonTorrent/Exercises_IonTorrent_day1.Rmd
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Expand Up @@ -52,13 +52,12 @@ Select all the FASTQ files:

Note that after this point in real life you can delete the individual FASTQ files, because you can always open the tar package using the tool `Utilities / Extract .tar.gz file`.

**Step 3. Check the quality of reads with FastQC**
**Step 3. Check the quality of reads with MultiQC**

Select the file `zippedFastq.tar` and the tool `Quality control / Read quality with MultiQC for many FASTQ files`, and click *Run*. Select the result file and click *Open in new tab*.

```
How long are the reads (in the General Statistics section, click
Configure columns, select Length and unselect M sequences)?
How long are the reads?
Based on the plot Sequence Counts, do all the samples have the
same number of reads? Are most of the reads unique?
Expand Down

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