How to merge biological replicates and do comparison analysis?

[kiwipeel]

Hi,

I have single cell data with 2 condition and 4 replicates in each condition.

In the tutorial why do we use directly cellchat object derived from only conditions? Why dont we create a cellchat object for each individual and merge them ?

I have tried to create cellchat object for each of my replicates and tried to merge them according to their conditions but it doesnt work.