forked from nf-core/modules
-
Notifications
You must be signed in to change notification settings - Fork 0
Commit
This commit does not belong to any branch on this repository, and may belong to a fork outside of the repository.
Merge branch 'master' into update-panaroo/run
* master: New module : Pilon (nf-core#3331) Islandpath (nf-core#3145)
- Loading branch information
Showing
12 changed files
with
272 additions
and
1 deletion.
There are no files selected for viewing
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,37 @@ | ||
process ISLANDPATH { | ||
tag "$meta.id" | ||
label 'process_medium' | ||
|
||
conda "bioconda::islandpath=1.0.6" | ||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? | ||
'https://depot.galaxyproject.org/singularity/islandpath:1.0.6--hdfd78af_0': | ||
'quay.io/biocontainers/islandpath:1.0.6--hdfd78af_0' }" | ||
|
||
input: | ||
tuple val(meta), path(genome) | ||
|
||
output: | ||
tuple val(meta), path("*.gff") , emit: gff | ||
path "Dimob.log" , emit: log | ||
path "versions.yml" , emit: versions | ||
|
||
when: | ||
task.ext.when == null || task.ext.when | ||
|
||
script: | ||
def args = task.ext.args ?: '' | ||
def prefix = task.ext.prefix ?: "${meta.id}" | ||
// WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. | ||
def VERSION = '1.0.6' | ||
""" | ||
islandpath \\ | ||
$genome \\ | ||
${prefix}.gff \\ | ||
$args | ||
cat <<-END_VERSIONS > versions.yml | ||
"${task.process}": | ||
islandpath: $VERSION | ||
END_VERSIONS | ||
""" | ||
} |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,44 @@ | ||
name: islandpath | ||
description: Genomic island prediction in bacterial and archaeal genomes | ||
keywords: | ||
- genomes | ||
- genomic islands | ||
- prediction | ||
tools: | ||
- "islandpath": | ||
description: "IslandPath-DIMOB is a standalone software to predict genomic islands (GIs - clusters of genes in prokaryotic genomes of probable horizontal origin) in bacterial and archaeal genomes based on the presence of dinucleotide biases and mobility genes." | ||
homepage: https://github.com/brinkmanlab/islandpath | ||
documentation: https://github.com/brinkmanlab/islandpath#readme | ||
tool_dev_url: https://github.com/brinkmanlab/islandpath | ||
doi: "10.1093/bioinformatics/bty095" | ||
licence: "['GPL v3']" | ||
|
||
input: | ||
- meta: | ||
type: map | ||
description: | | ||
Groovy Map containing sample information | ||
e.g. [ id:'test', single_end:false ] | ||
- genome: | ||
type: file | ||
description: | | ||
Genome file in .gbk or .embl format. | ||
pattern: "*.{gbk, embl, gbff}" | ||
output: | ||
- gff: | ||
type: file | ||
description: GFF file listing the predicted genomic islands and their coordinates | ||
pattern: "*.gff" | ||
- log: | ||
type: file | ||
description: Log file of the islandpath run | ||
pattern: "*.log" | ||
|
||
- versions: | ||
type: file | ||
description: File containing software versions | ||
pattern: "versions.yml" | ||
|
||
authors: | ||
- "@jvfe" |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,43 @@ | ||
process PILON { | ||
tag "$meta.id" | ||
label 'process_medium' | ||
|
||
conda "bioconda::pilon=1.24" | ||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? | ||
'https://depot.galaxyproject.org/singularity/pilon:1.24--hdfd78af_0': | ||
'quay.io/biocontainers/pilon:1.24--hdfd78af_0' }" | ||
|
||
input: | ||
tuple val(meta), path(fasta) | ||
tuple val(meta_bam), path(bam), path(bai) | ||
val pilon_mode | ||
|
||
output: | ||
tuple val(meta), path("*.fasta") , emit: improved_assembly | ||
tuple val(meta), path("*.vcf") , emit: vcf , optional : true | ||
tuple val(meta), path("*.change"), emit: change_record , optional : true | ||
tuple val(meta), path("*.bed") , emit: tracks_bed , optional : true | ||
tuple val(meta), path("*.wig") , emit: tracks_wig , optional : true | ||
path "versions.yml" , emit: versions | ||
|
||
when: | ||
task.ext.when == null || task.ext.when | ||
|
||
script: | ||
def args = task.ext.args ?: '' | ||
def prefix = task.ext.prefix ?: "${meta.id}" | ||
def valid_mode = ["frags", "jumps", "unpaired", "bam"] | ||
if ( !valid_mode.contains(pilon_mode) ) { error "Unrecognised mode to run Pilon. Options: ${valid_mode.join(', ')}" } | ||
""" | ||
pilon \\ | ||
--genome $fasta \\ | ||
--output ${meta.id} \\ | ||
--threads $task.cpus \\ | ||
$args \\ | ||
--$pilon_mode $bam | ||
cat <<-END_VERSIONS > versions.yml | ||
"${task.process}": | ||
pilon: \$(echo \$(pilon --version) | sed 's/^.*version //; s/ .*\$//' ) | ||
""" | ||
} |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,71 @@ | ||
name: "pilon" | ||
description: Automatically improve draft assemblies and find variation among strains, including large event detection | ||
keywords: | ||
- polishing | ||
- assembly | ||
- variant calling | ||
tools: | ||
- "pilon": | ||
description: "Pilon is an automated genome assembly improvement and variant detection tool." | ||
homepage: "https://github.com/broadinstitute/pilon/wiki" | ||
documentation: "https://github.com/broadinstitute/pilon/wiki/Requirements-&-Usage" | ||
tool_dev_url: "https://github.com/broadinstitute/pilon" | ||
doi: "https://doi.org/10.1371/journal.pone.0112963" | ||
licence: "['GPL v2']" | ||
|
||
input: | ||
- meta: | ||
type: map | ||
description: | | ||
Groovy Map containing sample information | ||
e.g. [ id:'test', single_end:false ] | ||
- fasta: | ||
type: file | ||
description: FASTA of the input genome | ||
pattern: "*.{fasta}" | ||
- bam: | ||
type: file | ||
description: BAM file of reads aligned to the input genome | ||
pattern: "*.{bam}" | ||
- bai: | ||
type: file | ||
description: BAI file (BAM index) of BAM reads aligned to the input genome | ||
pattern: "*.{bai}" | ||
- pilon_mode: | ||
type: value | ||
description: Indicates the type of bam file used (frags for paired-end sequencing of DNA fragments, such as Illumina paired-end reads of fragment size <1000bp, jumps for paired sequencing data of larger insert size, such as Illumina mate pair libraries, typically of insert size >1000bp, unpaired for unpaired sequencing reads, bam will automatically classify the BAM as one of the three types above (version 1.17 and higher). | ||
pattern: ["frags", "jumps", "unpaired", "bam"] | ||
|
||
output: | ||
- meta: | ||
type: map | ||
description: | | ||
Groovy Map containing sample information | ||
e.g. [ id:'test', single_end:false ] | ||
- versions: | ||
type: file | ||
description: File containing software versions | ||
pattern: "versions.yml" | ||
- improved_assembly: | ||
type: file | ||
description: fasta file, improved assembly | ||
pattern: "*.{fasta}" | ||
- change_record: | ||
type: file | ||
description: file containing a space-delimited record of every change made in the assembly as instructed by the --fix option | ||
pattern: "*.{change}" | ||
- vcf: | ||
type: file | ||
description: Pilon variant output | ||
pattern: "*.{vcf}" | ||
- tracks_bed: | ||
type: file | ||
description: files that may be viewed in genome browsers such as IGV, GenomeView, and other applications that support these formats | ||
pattern: "*.{bed}" | ||
- tracks_wig: | ||
type: file | ||
description: files that may be viewed in genome browsers such as IGV, GenomeView, and other applications that support these formats | ||
pattern: "*.{wig}" | ||
|
||
authors: | ||
- "@scorreard" |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,18 @@ | ||
#!/usr/bin/env nextflow | ||
|
||
nextflow.enable.dsl = 2 | ||
|
||
include { GUNZIP } from '../../../../modules/nf-core/gunzip/main.nf' | ||
include { ISLANDPATH } from '../../../../modules/nf-core/islandpath/main.nf' | ||
|
||
workflow test_islandpath { | ||
|
||
input = [ | ||
[ id:'test' ], // meta map | ||
file(params.test_data['bacteroides_fragilis']['genome']['genome_gbff_gz'], checkIfExists: true) | ||
] | ||
|
||
GUNZIP ( input ) | ||
|
||
ISLANDPATH ( GUNZIP.out.gunzip ) | ||
} |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,5 @@ | ||
process { | ||
|
||
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" } | ||
|
||
} |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,11 @@ | ||
- name: islandpath test_islandpath | ||
command: nextflow run ./tests/modules/nf-core/islandpath -entry test_islandpath -c ./tests/config/nextflow.config -c ./tests/modules/nf-core/islandpath/nextflow.config | ||
tags: | ||
- islandpath | ||
files: | ||
- path: output/islandpath/Dimob.log | ||
contains: | ||
- "Running IslandPath-DIMOB" | ||
- path: output/islandpath/test.gff | ||
md5sum: d4719f73e9af606346fade238aa191fa | ||
- path: output/islandpath/versions.yml |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,20 @@ | ||
#!/usr/bin/env nextflow | ||
|
||
nextflow.enable.dsl = 2 | ||
|
||
include { PILON } from '../../../../modules/nf-core/pilon/main.nf' | ||
|
||
workflow test_pilon { | ||
|
||
input = [ | ||
[ id:'test', single_end:false ], // meta map | ||
file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) | ||
] | ||
|
||
bam_tuple_ch = Channel.of([ [ id:'test', single_end:false ], // meta map | ||
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), | ||
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true), | ||
]) | ||
|
||
PILON ( input, bam_tuple_ch, "bam" ) | ||
} |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,5 @@ | ||
process { | ||
|
||
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" } | ||
|
||
} |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,8 @@ | ||
- name: pilon test_pilon | ||
command: nextflow run ./tests/modules/nf-core/pilon -entry test_pilon -c ./tests/config/nextflow.config -c ./tests/modules/nf-core/pilon/nextflow.config | ||
tags: | ||
- pilon | ||
files: | ||
- path: output/pilon/test.fasta | ||
md5sum: 2e881994820a5a641da9ea594ab4958f | ||
- path: output/pilon/versions.yml |