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Adding Restrander to the nanoseq pipeline for cDNA read orientation. This is documented in a GH ticket here.

Changes made to add Restrander to the nanoseq pipeline:

  • Added a Restrander module.
  • Added code to the nanoseq workflow to include the use of Restrander.
  • Updated modules and subworkflows downstream of Restrander to account for the inclusion of Restrander-related information in the channel.
  • Updated the check_samplesheet python file to account for the inclusion of the restrander_config column in the sample sheet.
  • Updated the usage and output docs to include information about Restrander.

Have tested the updated code under the following conditions and the pipeline ran successfully:

  • Run with cDNA and a Restrander config - ran normal cDNA workflow with addition of Restrander execution
  • Run with cDNA but no Restrander config - ran normal cDNA workflow and did not execute Restrander
  • Run with DNA - ran normally
  • Run with cDNA and mix of samples with and without Restrander configs - only ran Restrander for the files with configs present

thomas-watchmaker and others added 30 commits April 9, 2025 15:38
updated process_medium to process_high in fastqc main
Adding downsampling to our custom cut of nanoseq
…d which container the restrander module was pulling (from 1.0 to 1.1).
…sh: /bin/bash: cannot execute binary file error. Have updated the pulled image version to 1.2
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This PR is against the master branch ❌

  • Do not close this PR
  • Click Edit and change the base to dev
  • This CI test will remain failed until you push a new commit

Hi @julietmWM,

It looks like this pull-request is has been made against the watchmaker-genomics/nanoseq master branch.
The master branch on nf-core repositories should always contain code from the latest release.
Because of this, PRs to master are only allowed if they come from the watchmaker-genomics/nanoseq dev branch.

You do not need to close this PR, you can change the target branch to dev by clicking the "Edit" button at the top of this page.
Note that even after this, the test will continue to show as failing until you push a new commit.

Thanks again for your contribution!

@julietmWM julietmWM changed the base branch from master to dev August 12, 2025 14:17
@julietmWM julietmWM changed the base branch from dev to master August 12, 2025 14:19
@julietmWM julietmWM closed this Aug 12, 2025
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5 participants